• genes
  • HNRTs and deletions were correlated with qualitative changes in the expression of specific homoeologous genes and anonymous cDNA amplified fragment length polymorphisms and with phenotypic variation among S5 polyploids. (plantcell.org)
  • detected homoeologous rearrangements in resynthesized B. napus that altered the expression of parental FLC genes. (plantcell.org)
  • GIFs are characteristic fibroblasts with specific gene expressions from transcriptional regulation, signaling ligands, and extracellular matrix remodeling related genes. (sigmaaldrich.com)
  • Relative expression levels of genes in adapted P. atrosepticumcells vs. growing ones. (up.ac.za)
  • Thus, changes in expression and interactions in a core subset of genes may be critical for emergence of a healthy maternal phenotype, but inappropriate changes of the same genes could put women at risk for post-partum disorders. (frontiersin.org)
  • Although the understanding of ectopic expressions deals with endogenous genes in an organism, it can be expended to a similar concept like transgenesis, which an exogenous gene is introduced to a cell or tissue type in which the gene is not usually expressed. (wikipedia.org)
  • In a study, researchers identified ectopic expression of OR genes in non-olfactory tissues in the mouse model by measuring transcript levels. (wikipedia.org)
  • Genes with ectopic expression phenotypes are common, not rare" (PDF). (wikipedia.org)
  • The evo-geno approach was also used to verify the theory that humans and non-human primates share similar expression levels in energy metabolism-related genes which have implications for aging and neurodegenerative disease. (wikipedia.org)
  • Major gene characterizes common expression of oligogenic series, i.e. a small number of genes that determine the same trait. (wikipedia.org)
  • As one example, quorum sensing (QS) enables bacteria to restrict the expression of specific genes to the high cell densities at which the resulting phenotypes will be most beneficial. (wikipedia.org)
  • The Perturb-seq protocol uses CRISPR technology to inactivate specific genes and DNA barcoding of each guide RNA to allow for all perturbations to be pooled together and later deconvoluted, with assignment of each phenotype to a specific guide RNA. (wikipedia.org)
  • Due to the large size of these genes, a two-vector system can be used to express the endonuclease separately from the sgRNA expression vector. (wikipedia.org)
  • ZFIN's relational database interface provides query forms and display pages for the following biological data types: Genes, markers, and clones Gene expression Antibodies Sequence alignments (BLAST) Mutants and transgenic lines Anatomy Genetic maps ZFIN also maintains a database of zebrafish-related publications, laboratories, people, and companies. (wikipedia.org)
  • humans
  • Severe alphal-antitrypsin deficiency, as is seen with the rare phenotypes PiSZ and PiZZ, predisposes humans to emphysema and chronic liver disease. (docme.ru)
  • The results demonstrated that alphaj-antitrypsin-deficient phenotypes substantially predispose humans to chronic, bilateral, and recurrent anterior WAKEFIELD ET AL 1432 uveitis (severe uveitis), while HLA-B27 appears to act as an independent genetic predisposing factor in recurrent anterior uveitis. (docme.ru)
  • Ectopic expression of Drosophila homolog eyeless (ey) has been used to identify roles of Pax-6 in humans. (wikipedia.org)
  • Furthermore, LEKTI-knockout mice exhibit a phenotype similar to Netherton syndrome in humans. (wikipedia.org)
  • physiology
  • Although many studies describe the phenomenon of cross-protection and several regulatory components that induce the formation of resistant cells were elucidated, the global comparison of the physiology of cross-protected phenotype and growing cells has not been performed. (up.ac.za)
  • transcriptome
  • In the present study, we determined the period necessary for the formation of cross-protected Pba phenotype under starvation conditions, and compare the transcriptome profiles of non-adapted growing cells and of adapted cells after the cross-protective effect has reached the maximal level. (up.ac.za)
  • Perturb-seq is a reverse genetics approach that allows for the investigation of phenotypes at the level of the transcriptome, to elucidate gene functions in many cells, in a massively parallel fashion. (wikipedia.org)
  • transformants
  • and (3) stability of integration and expression of the transgenes was confirmed for all the transformants grown under natural environmental conditions. (gva.es)
  • Haploid wild-type yeast transformants containing a single copy of Mp SOD2 showed increased resistance phenotypes against oxidative stress-inducing hydrogen peroxide and paraquat, but had unaltered phenotype against ultraviolet-C (UVC) radiation. (mdpi.com)
  • cells
  • RT-PCR evidence for expression of D1, D2, and D5 in outer hair cells is not schematized here. (jneurosci.org)
  • By 5 d.p.f., when there is strong local expression of rag1-expressing T-cells in the thymus in the WT, mne lacks rag1 expression in the thymus despite development of the thymic primordia as marked by foxn1 (Fig. 2d). (nih.gov)
  • Despite normal specification of HSCs, as defined by cells expressing runx1 and myb along the ventral wall of the dorsal aorta (Supplementary Fig. 2b), myb expression is absent in mne caudal haemopoietic tissue at 72 and 96 h.p.f., suggesting that maintenance of the stem cell pool is also disrupted in mne (Supplementary Fig. 2c,d). (nih.gov)
  • Actin organization associated with the expression of multidrug resistant phenotype in osteosarcoma cells and the effect of actin depolymerization on drug resistance. (sigmaaldrich.com)
  • Nanotexturing of titanium-based surfaces upregulates expression of bone sialoprotein and osteopontin by cultured osteogenic cells (2003) Tambasco de Oliveira Paulo et al. (naver.com)
  • Thus, researchers have used the principle of ectopic expression to express SSX2 to different cell lines including cancer model cells. (wikipedia.org)
  • A flow cytometer instrument can examine the expression of molecules on individual cells in fluids. (wikipedia.org)
  • Droplet-based microfluidics platforms (or other cell sorting and separating techniques) are used to isolate individual cells, and then scRNA-seq is performed to generate gene expression profiles for each cell. (wikipedia.org)
  • Selection for successfully transduced cells is then performed using a fluorescence assay or an antibiotic assay, depending on the reporter gene used in the expression vector. (wikipedia.org)
  • specific
  • Functional expression of the StV-specific MERG1a channel reveals a current that activates at relatively negative potentials (approximately-50 mV) and shows rapid inactivation reflected as inward rectification at depolarized potentials. (mendeley.com)
  • However, early in haemopoietic development when primitive haemopoiesis prevails, mne displays a highly specific, lineage-restricted, myeloid phenotype. (nih.gov)
  • common
  • Mildly deficient phenotypes such as PiMZ and PiMS are quite common and are associated with a number of diseases, including rheumatoid arthritis (5-7), systemic lupus erythematosus (7), asthma (8), and fibrosing alveolitis (9). (docme.ru)
  • Some common phenotypes include biofilm formation, virulence factor expression, and motility. (wikipedia.org)
  • biological
  • The establishment of the maternal phenotype is one of the most dramatic biological transformations known in mature mammals. (frontiersin.org)
  • Practices of ectopic expression in biological science is not only limited to identifying a function of the gene in a known cell or tissue type but also implemented to discover unknown or additional functions of the gene by ectopic expression. (wikipedia.org)
  • found
  • Here they found relatively low OR gene expression compared to the olfactory tissue, which result indicates that the OR gene in other tissue have no extra function, but they suggest that there is a possibility that small OR subsets can have functional roles in different tissue. (wikipedia.org)
  • They found important phenotypes of ectopic SSX2 expression that is involved in tumorigenesis: 1) immediate induction of genomic instability, 2) long-term support of tumor cell growth. (wikipedia.org)
  • cell
  • Arrows indicate Zbtb11 expression in the intermediate cell mass (ICM). (nih.gov)
  • Abstract In the aortic wall of mammalian species, the maturation phase of smooth muscle cell (SMC) lineage is characterized by two temporally correlated but opposite regulatory processes of gene expression: upregulation of SM type SM2 myosin isoform and downregulation of brain (myosin heavy chain B)- and platelet (myosin heavy chain A pla )-type nonmuscle myosins. (ahajournals.org)
  • Ectopic expression using these techniques is a useful tool because phenotypes induced in a tissue or cell type where are not normally expressed are easily distinguishable compared to a tissue or cell type where the gene is normally expressed. (wikipedia.org)
  • Perturb-seq combines multiplexed CRISPR mediated gene inactivations with single cell RNA sequencing to assess comprehensive gene expression phenotypes for each perturbation. (wikipedia.org)
  • analysis
  • The combination of the microscopic examination of the peripheral blood and analysis of the lymphocytes by flow cytometry to confirm clonality and marker molecule expression is needed to establish the diagnosis of CLL. (wikipedia.org)
  • Thisse high-throughput gene expression analysis). (wikipedia.org)
  • similar
  • Hereditary inclusion body myopathies comprise both autosomal recessive and autosomal dominant muscle disorders that have a variable expression (phenotype) in individuals, but all share similar structural features in the muscles. (wikipedia.org)
  • disease
  • 143 1 IMMUNOGENETIC FACTORS IN INFLAMMATORY EYE DISEASE Influence of HLA-B27 and Alpha,-Antitrypsin Phenotypes on Disease Expression DENIS WAKEFIELD, SAMUEL N. BREIT, PEGGY CLARK, and RONALD PENNY The relationship between the nature and severity of inflammatory eye disease was analyzed with respect to HLA antigens and alphal-antitrypsin phenotypes. (docme.ru)
  • There was a significantly increased incidence of alphal-antitrypsinaeficient phenotypes in anterior uveitis, especially in those patients with severe (chronic, bilateral, or recurrent) disease. (docme.ru)
  • In the present study we analyzed the relationship between alphal-antitrypsin phenotype, HLA antigens, and the nature and severity of inflammatory eye disease. (docme.ru)