Mesenchymal stemDifferentiationChondrocytesGenesCartilageMSCsChondrocyteChondrogenicEmbryonicCellsNeuralMarkersDevelopmentalProtein expressionCondensationSox9CascadesSignificantlyUndergoTherapeuticBMSCsCollagen expressionDifferential gene expressionEmbryogenesisRolesTranscriptionInduceDiseaseEctopicTissuesGene expression profileTissueDevelopmentEnzyme activityReceptorAlterationsNuclearHighAdultMolecule
Mesenchymal stem3
- Mesenchymal stem cells (MSCs) are well known to have the capability to form bone and cartilage, and chondrogenesis derived from MSCs is reported to be affected by mechanical stimuli. (qigonginstitute.org)
- Methylation status of CpG islands in the promoter regions of signature genes during chondrogenesis of human synovium-derived mesenchymal stem cells. (nih.gov)
- Hyaluronic acid (HA) hydrogels formed via photocrosslinking provide stable 3D hydrogel environments that support the chondrogenesis of mesenchymal stem cells (MSCs). (umn.edu)
Differentiation18
- Noggin, a developmental protein, inhibits chondrogenesis by preventing condensation and differentiation of mesenchymal cells. (wikipedia.org)
- The protein expression of chondrocyte-specific markers of Aggrecan, Sox9, and BMP7 were upregulated and Collagen X was decreased by LMHF vibration introduced at the chondrogenic differentiation in vitro. (qigonginstitute.org)
- Our data demonstrated that LMHF mechanical vibration promotes BMSCs chondrogenic differentiation and implies β-catenin signal acts as an essential mediator in the mechano-biochemical transduction and subsequent transcriptional regulation in the process of chondrogenesis. (qigonginstitute.org)
- RA receptor (RAR)-gamma expression is essential for vertebrate chondrocyte differentiation. (europa.eu)
- The HUCMSCs derived multiple donors were performed comprehensively analysis and potent assays including expressions of surface markers, viability, growth curve, karyotype analysis, tumorigenicity, differentiation potentials, and immune regulation capability. (researchsquare.com)
- The HUCMSCs derived multiple donors kept a high consistency in surface marker expressions, viability, growth curve, tumorigenicity in nude mic, but had robust heterogeneities in differentiation potentials and immune regulations. (researchsquare.com)
- In spite of tissue origin, many other elements such as culture method and further modulations also influence the heterogeneity of MSCs, leading to differential gene expression profiles, growth phenotype, and differentiation potentials [15-17]. (researchsquare.com)
- Using a defined in vitro model of early chondrogenesis, we attempted to enrich for cells with an enhanced ability for chondrogenic differentiation by pre-exposure of mouse adipose-derived adult stromal cells (ADASs) to a hypoxic (2% oxygen) environment. (ox.ac.uk)
- Furthermore, re-oxygenation of the 2% oxygen-expanded ADASs before differentiation did not significantly affect early chondrogenesis. (ox.ac.uk)
- In this study, we assayed the effect of exogenous mouse recombinant GDF-5 on cell proliferation of dental pulp cells and determined the expression levels of odontoblast differentiation marker genes in the cells of isolated mouse dental pulp tissues in the presence of GDF-5. (ispub.com)
- Here, we investigated the effect of GDF-5 on the proliferation of dental pulp cells and their differentiation into odontoblasts by assaying the expression levels of odontoblast marker genes. (ispub.com)
- The receptor tyrosine kinase (RTK)-like orphan receptor (ROR)2 belongs to a conserved family of tyrosine kinase receptors that play important roles in some developmental processes, such as chondrogenesis, osteoblastogenesis, and neural differentiation [ 6 , 7 ]. (oncotarget.com)
- BMPR1A and BMPR1B are vital for osteoblast differentiation and chondrogenesis impacting bone remodeling. (news-medical.net)
- To evaluate the effects of applied cytomechanical forces towards cellular differentiation distinct parameters were measured (morphology, antigen and antigen expression) after a total cultivation period of 21 days in vitro. (biomedcentral.com)
- For the quantification of cellular differentiation at the molecular level, osteogenic differentiation of MSCs is controlled by the interaction of hormones and transcription factors: runt-related transcription factor-2 (RUNX2) effectuates the expression of bone-specific genes, e.g. osterix (OSX), collagen type 1 alpha-1 (COL1A1), osteocalcin (OC), and bone sialoprotein (BSP) by binding to the promoters of these genes. (biomedcentral.com)
- Moreover, miR-30a was revealed to be up-regulated during chondrogenic differentiation of BMSCs, and miR-30a can promote this process by inhibiting delta-like 4 expression [45]. (bibf1120.com)
- Objective: To establish cultures of cells from the pulp of permanent teeth by the explant method assessing parameters usually presented by stem cells, such as the expression of certain markers and the differentiation ability into osteogenic, adipogenic, and chondrogenic lineages. (bvsalud.org)
- The isolated cells may be considered stem cells, based on the current criteria for their characterization, such as plastic adherence, expression of certain markers, and the absence of others, as well as multi-differentiation potential, which showed to be promising for the application in tissue regeneration. (bvsalud.org)
Chondrocytes4
- Chondrification (also known as chondrogenesis) is the process by which cartilage is formed from condensed mesenchyme tissue, which differentiates into chondrocytes and begins secreting the molecules that form the extracellular matrix. (wikipedia.org)
- The expression of Sox9 induces the expression of BMP, which causes chondrocytes to proliferate and differentiate. (wikipedia.org)
- L-Sox5 and Sox6 are thought to induce the activation of the Col2a1 and the Col11a2 genes, and to repress the expression of Cbfa1, a marker for late stage Chondrocytes. (wikipedia.org)
- Spatial expression analysis of both genes revealed that Sof-RXR is expressed ubiquitously in all cells of the embryo, including the chondrocytes, whereas Sof-ALDH was expressed only in a sub-set of epithelial cells and no expression at the level of chondrocytes was observed. (europa.eu)
Genes18
- Research is still ongoing and novel transcription factors, such as ATOH8 and EBF1, are added to the list of genes that regulate chondrogenesis. (wikipedia.org)
- Gene expression analysis indicates that esrra is expressed in developing pharyngeal arches where genes necessary for cartilage development are also expressed. (nature.com)
- Loss of function analysis shows that knockdown of esrra impairs expression of genes including sox9, col2a1, sox5, sox6, runx2 and col10a1 thus induces abnormally formed cartilage in pharyngeal arches. (nature.com)
- Expression of esrra is colocalised with genes necessary for cartilage development in pharyngeal arches during zebrafish embryogenesis. (nature.com)
- A further 80 genes showed greater than 5 fold differences in expression after exposure to dragonfly larvae and 81 genes showed altered expression after exposure to larval salamanders. (biomedcentral.com)
- Predation-threat responsive genes were identified by selecting genes that reverted to control levels of expression following removal of the predator. (biomedcentral.com)
- The higher expression of gremlin 1 and HIF1a genes after exposure to dragonfly larvae indicated an in vivo hypoxic reaction, while down-regulation of syndecan-2 may indicate impairment of angiogenesis. (biomedcentral.com)
- the tadpoles showed reduced expression of cα(E)-catenin , small muscle protein, dystrophin , and myosin light chain genes. (biomedcentral.com)
- It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. (thermofisher.com)
- The gene expression of Ppargc1a varied during scleral growth from week 1 to 2, week 2 to 3, week 3 to 6, and week 6 to 8 and was found to interact with a different set of genes at different scleral growth stages. (molvis.org)
- DAVID was used to perform the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis for the different expression genes (DEGs). (medscimonit.com)
- To directly test this model of Shh function, we have restored cell survival in Shh mutant embryos after transient Shh expression by concomitant removal of the pro-apoptotic genes Bak/Bax to intercept cell death, in order to rescue the proposed late Shh role. (nih.gov)
- Expression of two nonallelic type II procollagen genes during Xenopus laevis embryogenesis is characterized by stage-specific production of alternatively spliced transcripts. (rupress.org)
- At later times and coincident with the appearance of chondrogenic tissues in the developing embryo, expression of the procollagen genes is characterized by the production of an additional, alternatively spliced transcript. (rupress.org)
- Compared with non-tumorous tissues, both mRNA expression and protein product of ROR2 and Wnt5a genes were significantly increased in NSCLC. (oncotarget.com)
- It may regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. (neobiotechnologies.com)
- Recently, miRNAs were also demonstrated to be involved in the process of chondrogenesis by interacting with several targetted genes [42,43]. (bibf1120.com)
- Our gene expression analyses were unable to identify type II collagen in the sea lamprey hyaline cartilage nor any other fibrillar collagen during chondrogenesis at the stages observed, meaning that sea lamprey likely no longer require these genes during early cartilage development. (nsf.gov)
Cartilage5
- Chondrogenesis is the process by which cartilage is developed. (wikipedia.org)
- Accordingly, ectopic expression of sox9 rescues defective formation of cartilage induced by the knockdown of esrra . (nature.com)
- Taken together, our results indicate for the first time that ESRRa is essential for cartilage development by regulating sox9 expression during vertebrate development. (nature.com)
- Along with other BMPs, including BMP-2, -4 and -7, which are well known as potent osteoinductive growth factors, GDF-5 plays important roles in the development of bones, cartilage and tendons, as evidenced by the tendency for the gene and protein expression levels of GDF-5 to increase over time in these tissues. (ispub.com)
- Our data, by showing the role of IGF-I and TGF-β1 in the critical steps of MSC chondrogenesis, provide critical information to optimize the therapeutic use of MSCs in cartilage disorders. (elsevierpure.com)
MSCs6
- MSCs undergoing chondrogenesis are preferentially responsive to an electromagnetic efficacy window defined by field amplitude, duration and frequency of exposure. (nature.com)
- This correlated with enhanced chondrogenesis and a reduction in the expression of markers associated with chondrocyte hypertrophy, as well as increased SMAD 2/3 nuclear localization in the encapsulated MSCs. (amrita.edu)
- Furthermore, we assessed the global gene expression profile of MSCs on uncoated and PLL-coated plates. (hindawi.com)
- MSCs on PLL-coated plates exhibited a faster growth rate with increased S-phase and upregulated expression of the stemness markers. (hindawi.com)
- These MSCs derived different tissues do not have uniform characteristics, differing in expression profiles of surface markers and biological functions after certain stimulation such as pro-inflammatory mediators [5, 13]. (researchsquare.com)
- We obtained MSCs from mice in which green fluorescent protein (GFP) was under the Collagen2 promoter and determined GFP expression by confocal microscopy. (elsevierpure.com)
Chondrocyte2
- Ihh stimulates chondrocyte proliferation and regulates chondrocyte maturation by maintaining the expression of PTHrP. (wikipedia.org)
- Results and Conclusions: IGF-I modulated MSC chondrogenesis by stimulating proliferation, regulating cell apoptosis, and inducing expression of chondrocyte markers. (elsevierpure.com)
Chondrogenic2
- At later stages of development and parallel to increased mRNA accumulation, collagen expression becomes progressively more confined to chondrogenic regions of the tadpole. (rupress.org)
- In addition, miR-145 and miR-495 were reported to be down-regulated, and they can both inhibit chondrogenesis by interacting with the critical chondrogenic transcription factor, SOX9 [46,47]. (bibf1120.com)
Embryonic2
- Bone morphogenetic proteins are growth factors released during embryonic development to induce condensation and determination of cells, during chondrogenesis. (wikipedia.org)
- L-Sox5 is also thought to be involved primarily in embryonic chondrogenesis, while Sox6 is thought to be involved in post-natal chondrogenesis. (wikipedia.org)
Cells6
- In the present study, the potential of using exosomes isolated from adipose‑derived stem cells (ADSCs) as a therapeutic tool for reducing chronic inflammation and promoting chondrogenesis was investigated using patient‑derived primary cells. (spandidos-publications.com)
- In vitro expansion of adipose-derived adult stromal cells in hypoxia enhances early chondrogenesis. (ox.ac.uk)
- Gene expression analysis suggested that matrix metalloproteinase synthesis was inhibited in cells expanded in 2% oxygen. (ox.ac.uk)
- This study also aimed to assess the expression of ALDH1 (aldehyde dehydrogenase 1) enzyme activity on the isolated cells. (bvsalud.org)
- Cells were tested for the expression of surface markers and for ALDH1 enzyme activity, by flow cytometry. (bvsalud.org)
- Results: The isolated cells showed high expression of CD44 (98.8%), CD73 (100%), and CD90 (97.2%), and moderate expression of STRO-1 (18.4%) and ALDH1 (16.2%), by flow cytometry. (bvsalud.org)
Neural1
- During the early period of mRNA accumulation, there is also a transient pattern of expression in localized sites that will later not undergo chondrogenesis, such as the floor plate in the ventral neural tube. (rupress.org)
Markers2
- However, this was accompanied by increased markers of chondrogenesis. (qigonginstitute.org)
- Specifically,treatment of primary limb bud cultures with p38 inhibitors increases the expression of myogenic markers and causes a striking increase in formation of myotubes, which were detected using antibodies specific for myosin heavy chain. (biologists.com)
Developmental2
- A complex expression pattern of ESRRs during animal development seems to be consistent with the potential roles for ESRRa during appropriate developmental programs of tissues and organs in mouse and zebrafish 9 . (nature.com)
- We are analyzing the normal developmental function of early transcription factors that regulate digit patterning (Gli3, 5'Hoxd), with the long-term aim of linking regulatory cascades and patterned gene expression to the morphogenesis of specific structures. (nih.gov)
Protein expression1
- Depending on the cultivation technique we found significant differences in both gen and protein expression. (biomedcentral.com)
Condensation1
- H. Kwon, Y. Ohmiya, K. Honma, S. Honma, T. Nagai, K. Saito and K. Yasuda, Synchronized ATP oscillations have a critical role in prechondrogenic condensation during chondrogenesis. (atto.co.jp)
Sox91
- Importantly, we identify putative ESRRa binding elements in upstream regions of sox9 to which ESRRa can directly bind, indicating that Esrra may directly regulate sox9 expression. (nature.com)
Cascades1
- Pulse electromagnetic fields (PEMFs) have been shown to recruit calcium-signaling cascades common to chondrogenesis. (nature.com)
Significantly2
- c 2 analysis revealed that high ROR2 or Wnt5a expression in NSCLC was significantly associated with advanced TNM stage. (oncotarget.com)
- In the present study, we found that miR-134 expression was significantly down-regulated during chondrogenesis, and this process can be promoted in BMSCs treated with miR-134 inhibitor. (bibf1120.com)
Undergo1
- Tissue samples collected from the anterior glenohumeral joint capsule during surgery will undergo RNA-seq to determine differences in gene expression between the study groups. (biomedcentral.com)
Therapeutic1
- This study highlights the intricacies of calcium homeostasis during early chondrogenesis and the constraints that are placed on PEMF-based therapeutic strategies aimed at promoting MSC chondrogenesis. (nature.com)
BMSCs1
- The chondrogenesis process of BMSCs can be directed by multiple stimulus, such as growth factors, cellCmatrix conversation, and mechanical loads [41]. (bibf1120.com)
Collagen expression3
- The pattern of type II collagen expression during Xenopus laevis embryogenesis has been established after isolating specific cDNA and genomic clones. (rupress.org)
- Type II collagen expression is therefore temporally regulated by tissue-specific transcription and splicing factors directing the synthesis of distinct molecular forms of the precursor protein in the developing Xenopus embryo. (rupress.org)
- For example, type X collagen expression in the high crosslinking density 5% 15 min group was ∼156 and 285% higher when compared to the low crosslinking density 1% 15 min and 5% 5 min groups on day 42, respectively. (umn.edu)
Differential gene expression1
- This study provides a differential gene expression profile between TMJ fibrous and bony ankylosis. (medscimonit.com)
Embryogenesis1
- Expression of the mCherry transgene reporter during embryogenesis and early larval development faithfully replicated known expression domains of Hh-signaling in zebrafish, and abrogating Hh-signaling in transgenic fish resulted in the suppression of reporter expression. (zfin.org)
Roles2
- We focus our attention on three families of transcription factors that are known to have roles in vertebrate chondrogenesis: Sox and the Ant- and Prd- class families of the homeodomain proteins. (europa.eu)
- In addition we have investigated components of the retinoic acid and hedgehog signalling pathways, both of which have relevant roles in vertebrate chondrogenesis. (europa.eu)
Transcription3
- In the ventral limb bud, the transcription factor engrailed-1 (En-1) is produced. (medscape.com)
- Thus, understanding how normal transcription programs are regulated during development may also help to decipher abnormal gene expression patterns in tumors and devise new strategies to intercept cellular targets driving tumor cell behavior. (nih.gov)
- Previous studies revealed that SMADs could regulate the corresponding gene expressions by interacting with transcription factors in the nucleus when TGF- signaling is usually activated [48C50]. (bibf1120.com)
Induce1
- demonstrated the ability Rock2 of c-Kit signaling to induce expression. (bioinf.org)
Disease1
- Gene expression of eye diseases should be studied as early as postnatal weeks 1-2 to ensure that any changes in gene expression pattern during disease development are detected. (molvis.org)
Ectopic1
- Moreover, ectopic shh expression in Tg(Glid:mCherry) fish led to increased transgene production. (zfin.org)
Tissues2
- The differences in gene expression profiles induced by the two predators suggest that there are functional differences between the altered tail tissues of the two groups of tadpoles. (biomedcentral.com)
- mRNA expression of ROR2 and Wnt5a was examined in 20 pairs of NSCLC and matched adjacent normal tissues by real-time PCR. (oncotarget.com)
Gene expression profile1
- Comparative gene expression profile analysis between native human odontoblasts and pulp tissue. (nih.gov)
Tissue1
- Tissue microarray-based immunohistochemical analysis was performed to determine the expression of ROR2 and Wnt5a in 219 patients. (oncotarget.com)
Development3
- Relative levels of gene expression throughout development were determined by quantitative polymerase chain reaction (PCR) analysis and in cases where sufficient specific gene sequence was acquired, ribonucleic acid (RNA) probes were generated to evaluate the spatial expression patterns during the period of organogenesis. (europa.eu)
- Here we show that, concurrent with a decrease in chondrogenesis, inhibition of p38 in limb bud cultures dramatically promotes muscle development. (biologists.com)
- Of significance a functional redundancy between Snai1 and Snai2 in both chondrogenesis and cranial-facial development has been previously shown [17 18 Recently two laboratories including our own have described the generation of deficient animals. (bioinf.org)
Enzyme activity1
- GDF-5 also increased the gene expression and enzyme activity of alkaline phosphatase. (ispub.com)
Receptor1
- We investigated the expression of receptor tyrosine kinase-like orphan receptor (ROR) 2 and Wnt5a and their prognostic significance in non-small cell lung cancer (NSCLC). (oncotarget.com)
Alterations1
- Alterations occurring in BMPR expression and activity add up to cardiovascular diseases, fibrotic disorders, and skeletal abnormalities. (news-medical.net)
Nuclear1
- The expression and nuclear translocation of β-catenin were activated in a significant manner. (qigonginstitute.org)
High2
- High expression of both ROR2 and Wnt5a was also related to advanced TNM stage. (oncotarget.com)
- Our clinical findings suggest that high ROR2 or Wnt5a expression is associated with poor prognosis in NSCLC, and combined detection of ROR2 and Wnt5a is helpful in predicting the prognosis of NSCLC. (oncotarget.com)
Adult1
- Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain. (thermofisher.com)
Molecule1
- It was suggested that Tgfβ1 could possibly act as a signaling molecule in controlling collagen gene expression and modulate ECM during ocular growth. (molvis.org)