Experimental technique embryo culture techniques. Medical search. Frequent questions

Today, this technique continues to form the foundation for research on mammalian embryos, including technologies such as transgenic engineering, embryonic stem cell therapy, human in vitro fertilization, mammalian cloning, and knockout engineering. (avma.org)
We will provide hands-on training of basic laboratory techniques to produce and maintain specific mouse strains, including highly efficient superovulation, in vitro fertilization, cryopreservation of mouse sperm and embryos. (riken.jp)

The two enrichment strategies allow probing of methyl-CpG protein interactions in early vertebrate oocytes and embryos. (biomedcentral.com)
If all requirements for round spermatid selection and injection are successfully met, the injected oocytes develop to early embryos and can be transferred to the mother's uterus to produce pregnancy. (wikipedia.org)
Studies using Xenopus cell-free extracts, oocytes, eggs, embryos, larval stages, and adult frogs have yielded important insights into a multitude of key biological processes-from mechanisms underlying the cell cycle to embryonic development to human disease. (cshlpress.com)

For a reverse experiment, DNA methylation in early Xenopus embryos was assessed by MBD affinity capture. (biomedcentral.com)
This new laboratory manual provides a comprehensive collection of experimental procedures for research using Xenopus . (cshlpress.com)

Samuel Randall Detwiler was an embryologist who studied neural development in embryos and vertebrate retinas. (asu.edu)

When few biologists studied the biochemistry of embryos, Ebert built programs and courses around the foci of biochemistry and genetics, especially with regards to embryology. (asu.edu)

From there, Dr. Brinster became interested in modifying the development of animals and their germ lines, and he went on to become the first person to show that it was possible to colonize a mouse blastocyst with stem cells from older embryos. (avma.org)

Even though many technical aspects of ROSI are similar to those of ICSI, there are also significant differences between both techniques. (wikipedia.org)
After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)

We will provide hands-on training of basic laboratory techniques to produce genetically-modified mouse strains, including mouse zygote thawing, electroporation, and embryo transfer. (riken.jp)

Research advocates attack President Bush for "banning stem cell research," while pro-life advocates lament a Republican administration and Congress that have banned nothing-not embryo destruction, not human cloning, not fetal farming, not genetic engineering. (eppc.org)
But we can only wonder about the ethical propriety of producing the first human child with this technique, knowing that the hoped-for newborn would be a reproductive experiment, one that may end initially in numerous fetal failures. (eppc.org)
Powerful advancements in molecular genetic manipulation and assisted reproductive technolo-gies are employed during embryo and fetal development, and these efforts have had profound impact on animal production worldwide. (ac.ir)

According to the The Herald, he was the first, alongside his colleague Martin Evans, to isolate and culture embryonic stem cells. (asu.edu)
Before leaving office, President Clinton sought to get around the existing law without actually changing it, by funding research on embryonic stem cells so long as the actual embryo destruction was paid for with private dollars. (eppc.org)

Experimental Embryology is often referred to as a classical approach of developmental biology that has been to some extent replaced by the introduction of molecular biology and genetic techniques to the field. (mdpi.com)

In July 2005, for example, scientists announced that they had engineered adult mouse stem cells into usable mouse eggs, a technique that might one day allow for the creation of human eggs from ordinary human cells. (eppc.org)
And if post-menopausal women begin having children by producing eggs from other parts of their body, we will only aid the revolt against the lifecycle that now defines modern culture. (eppc.org)
Using this method of embryo manipulation, he next worked out many aspects of the metabolism and development of eggs and early embryos. (avma.org)

Citizens disagree about whether we should destroy human embryos for their stem cells-and if so, which embryos, with whose money, under what regulatory guidelines. (eppc.org)
And recent experimental trials, while still very preliminary, suggest that adult stem cells may one day help us treat a host of terrible pathologies. (eppc.org)
Far more controversial-and for good reason-are stem cells derived from destroyed human embryos. (eppc.org)

The organizer discovery was a crucial contribution to embryology that led to further understanding of the pattern of embryo differentiation of amphibians. (asu.edu)
Little is known about the developmental origin, determination and differentiation of different pineal immunoreactive cells in the avian group, and an experimental establishment is then required to explain the differentiation of cell types (i.e. photosensory, neural and secretory types). (bioone.org)

The experimental manipulation of mid-gestation mouse embryos is an important tool for the study of developmental biology. (ed.ac.uk)

It was noted that as opposed to the flattened morphology typically seen in 2D culture, cells grown on the electrospun fibers exhibited a more rounded 3-dimensional morphology generally observed of tissues in vivo. (wikipedia.org)
Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)

Round spermatid injection (ROSI) is a technique of assisted reproduction whereby a round spermatid is injected into oocyte cytoplasm in order to achieve fertilization. (wikipedia.org)

Tong's administrative and scientific leadership in the fields of marine, cellular, and developmental biology contributed to China's experimental embryology research programs. (asu.edu)
Inspired by the combination of this approach with advanced techniques to uncover core principles of neural crest development by the laboratory of Roberto Mayor, we review key quantitative examples of experimental embryology from recent work in a broad range of developmental biology questions. (mdpi.com)

Tissue culture is the growth of tissues or cells in an artificial medium separate from the parent organism. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture. (wikipedia.org)
He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
The technique of plant tissue culture, i.e., culturing plant cells or tissues in artificial medium supplemented with required nutrients, has many applications in efficient clonal propagation (true to the type or similar) which may be difficult via conventional breeding methods. (wikipedia.org)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
We propose that quantitative experimental embryology offers essential ways to explore the reaction of cells and tissues to targeted cell addition, removal, and confinement. (mdpi.com)

The first is organ culture where whole organs from embryos or partial adult organs are used to initiate the organ culture in vitro. (wikipedia.org)

In the second technique, morpholino oligonucleotides are electroporated into the pharyngeal region of the embryo at E10.5, creating an efficient system for the knockdown of gene function in the target cells. (ed.ac.uk)

Leo Loeb developed an experimental approach to studying cancer and pioneered techniques for tissue culture and in vitro tissue transplantation which impacted early-to-mid twentieth century experimental embryology. (asu.edu)
Dizhou Tong, also called Ti Chou Tung, studied marine animals and helped introduce and organize experimental embryology in China during the twentieth century. (asu.edu)

This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal uterus. (wikipedia.org)
These two trials aimed to explore and describe the biological effects that some important nutrients, such as proteins, lipids, fatty acids and carotenoids, have on urchins' somatic and gonadal growth, gonad biochemical composition during gametogenesis, fecundity and maternal provisioning to developing embryos. (stir.ac.uk)

These studies have combined peptide affinity pull-downs and quantitative mass spectrometry enabled by SILAC ( S table I sotope L abeling by A mino Acids in C ell Culture) [ 32 ]. (biomedcentral.com)
Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)

In modern usage, "Tissue culture" generally refers to the growth of cells from a multicellular organism in vitro. (wikipedia.org)
The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival. (wikipedia.org)
Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. (wikipedia.org)
In animal tissue culture, cells may be grown as two-dimensional monolayers (conventional culture) or within fibrous scaffolds or gels to attain more naturalistic three-dimensional tissue-like structures (3D culture). (wikipedia.org)
Tissue culture is used in creating genetically modified plants, as it allows scientists to introduce DNA changes to plant tissue via Agrobacterium tumefaciens or a gene gun and then generate a full plant from these modified cells. (wikipedia.org)
These cells retain their differentiated character and functional activity in organ culture. (wikipedia.org)
This culture is known as a primary explant, and migrating cells are known as outgrowth. (wikipedia.org)
The holy grail of regenerative medicine-whatever one's ethical beliefs about destroying embryos-is to "reprogram" regular cells from one's own body so that individuals can be the source of their own rejection-proof therapies. (eppc.org)
That is to say, we risk turning developed cells into developing embryos, and thus risk engaging in the very activities of embryo destruction and human cloning that we seek to avoid. (eppc.org)
Human iPS cells cannot be cultured with ordinary culture method and it requires special skills. (riken.jp)
This course presents basic methods to maintain Arabidopsis T87 and Tobacco BY2 suspension cultured cells (day 1) and applied methods for transformation technology of T87 cell (day 2). (riken.jp)
Trainees will learn the basis and procedure for cryopreservation of plant cultured cells in liquid nitrogen. (riken.jp)
In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
Low-quality embryos, on the other cells, thereby maintaining a relatively low basal hand, frequently display morphological level [16-18]. (who.int)

Recording and contextualizing the science of embryos, development, and reproduction. (asu.edu)
Described here is an example of a whole embryo culture system, where a serum-free medium is used to support the development of mouse embryos in vitro from embryonic day 10.5 (E10.5) to E11.5. (ed.ac.uk)
Once fertilized, the egg is transformed into a pre-embryo and it has to be transferred to the uterus to continue its development. (wikipedia.org)
Reasons for the delayed development of in vivo and in vitro production of equine embryos include the scarce availability of abattoir ovaries and the lack of interest from horse breeders and registries. (ivis.org)

A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Thus, simplified biological systems have become an inherent component of animal experimental research. (mdpi.com)
Since 1995, Congress has annually reauthorized a law-called the "Dickey Amendment"-prohibiting federal funding for research "in which" embryos are destroyed while leaving embryo destruction in the private sector entirely unregulated. (eppc.org)

Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
There are three common methods to establish cell culture from animals. (wikipedia.org)

In the first, protein-soaked beads are carefully positioned in the pharyngeal region of an E10.5 embryo, allowing the concentration of specific proteins to be altered within the tissue. (ed.ac.uk)
Although simple, this technique only provides an indication of the coexistence of two proteins. (jneurosci.org)
With the O-PTIR technique, researchers can follow the aggregation of Alzheimer's proteins in fresh samples of the brain tissue. (lu.se)

These techniques demonstrate the use of in vitro techniques to study organogenesis within the pharyngeal region of the mouse embryo, but with some modification they could be adapted to target any region of the endodermal gut tube. (ed.ac.uk)

However, other procedures based on the in vivo and in vitro production of equine embryos have emerged in recent years. (ivis.org)

The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. (wikipedia.org)
In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
In 1996, the first use of regenerative tissue was used to replace a small length of urethra, which led to the understanding that the technique of obtaining samples of tissue, growing it outside the body without a scaffold, and reapplying it, can be used for only small distances of less than 1 cm. (wikipedia.org)
On the other hand, the strict meaning of "tissue culture" refers to the culturing of tissue pieces, i.e. explant culture. (wikipedia.org)
This early use of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types would adhere to and proliferate upon polycarbonate fibers. (wikipedia.org)
Plant tissue culture in particular is concerned with the growing of entire plants from small pieces of plant tissue, cultured in medium. (wikipedia.org)
The second method is primary explant culture, in which fragments derived from animal tissue are attached to a surface using an extracellular matrix component (ECM), such as collagen or a plasma clot. (wikipedia.org)
Moreover, the researchers can detect associated damage at the submicronscale, something that makes it possible to study potential treatments in acute brain tissue slices, organoids, or cultured neurons. (lu.se)
Tissue engineering for esophageal replacement, in-utero intervention, and minimally invasive techniques such as thoracoscopy and robotic assistance may eventually improve treatment of these infants further. (medscape.com)

For his doctoral thesis, Dr. Brinster developed the first reliable in vitro culture system for early mammalian embryos. (avma.org)

We believe that the proposed compilation of publications will improve our understanding of certain physiological, genetic, and metabolic processes, but will also highlight the most recent cellular techniques that might be adapted in the laboratories. (mdpi.com)
This technique can be used to enable genetic fatherhood to some men who have no spermatozoa in the ejaculate (azoospermia) and in whom spermatozoa cannot be obtained surgically from the testicles. (wikipedia.org)

The procedures of the vitrification method are specific and quite different from the procedures of conventional slow-cooling method which is most commonly used in cell culture. (riken.jp)

We used two main experimental approaches. (jneurosci.org)

However, such techniques can be challenging due to difficulties accessing the embryos in utero, and therefore the ability to maintain mid-gestation mouse embryos in vitro has proved invaluable. (ed.ac.uk)
This makes it an ideal environment in which to support and maintain mid-gestation mouse embryos following experimental manipulations. (ed.ac.uk)

Embryos produced outside the breeding season can be frozen for later transfer, which makes these techniques very versatile. (ivis.org)

But we need to proceed carefully, recognizing that we are gaining new powers over human origins even when we do not use human embryos, and recognizing the danger of blurring the line between cellular parts and embryonic wholes. (eppc.org)

Frogs are easy to rear and maintain, and large sample sizes of embryos and later stages are readily obtained. (cshlpress.com)

This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
This technique has been used by MultiPark researchers in a new study led by Oxana Klementieva. (lu.se)

Solomon A. Berson helped develop the radioimmunoassay (RIA) technique in the US during the twentieth century. (asu.edu)

The melatonin level in the culture medium presented a high value up to 72 hr of culture, followed by a decrease as well as dampening of the level at the end of the culture possibly because the cultures were maintained in dark. (bioone.org)

Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)

Author HJ contributed to experimental work. (who.int)

The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cel embryos (p=0.04). (who.int)
As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
Due to its clinical applications success of IVF results is the morphological and the expansion of non-invasive treatment quality of the embryo. (who.int)

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