• The hepatocytes exhibited functional hepatic characteristics such as glycogen storage, indocyanine green uptake and release, and albumin secretion. (news-medical.net)
  • The increased availability of these hepatocytes helps researchers develop new drugs for hepatic infectious diseases and ADME Tox models. (massbio.org)
  • Since hepatic stem cells (HepSCs) possess self-renewal and bipotency with the capacity to differentiate into both hepatocytes and cholangiocytes, they have therapeutic potential for treating liver disease. (figshare.com)
  • Hepatocyte-like cells derived from iHepSCs (iHepSC-HEPs) exhibit the typical morphology of hepatocytes and hepatic functions, including glycogen storage, low-density lipoprotein (LDL) uptake, Indocyanine green (ICG) detoxification, drug metabolism, urea production, and albumin secretion. (figshare.com)
  • If a patient is asymptomatic, exhibits isolated liver disease, and lacks corneal rings, the coexistence of a hepatic copper concentration of more than 250 mg/g of dry weight and a low serum ceruloplasmin level is sufficient to establish a diagnosis. (medscape.com)
  • In this research, we performed transcriptomics analysis on the effect of fenofibrate in mice with hepatocyte-humanized livers and compared the results with other relevant transcriptomics datasets. (wur.nl)
  • Comparison with other transcriptomics datasets indicated that hepatocyte humanized livers recapitulate the principal effects of PPARα activation on lipid metabolism as revealed by other model systems of human liver. (wur.nl)
  • In contrast, pathways connected to DNA synthesis were downregulated by fenofibrate in chimeric mice with hepatocyte humanized livers yet upregulated by fenofibrate in normal mouse livers. (wur.nl)
  • As with other tissues, there is a scarcity of donor livers and hepatocytes, which is compounded by the low recovery and proliferative capacity of adult hepatocytes. (news-medical.net)
  • We analyzed immunoglobulins (Ig) in explanted livers from SAH patients (n=45) undergoing liver transplantation and tissue from corresponding healthy donors (HD, n=10) and found massive deposition of IgG and IgA isotype antibodies associated with complement fragment C3d and C4d staining in ballooned hepatocytes in SAH livers. (elifesciences.org)
  • Ig extracted from SAH livers, but not patient serum exhibited hepatocyte killing efficacy in an antibody-dependent cell-mediated cytotoxicity (ADCC) assay. (elifesciences.org)
  • In characterizing the molecular differentiation of developing peripheral auditory structures, we discovered that hepatocyte growth factor ( Hgf ) is expressed in the future stria vascularis of the cochlear epithelium. (jneurosci.org)
  • Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
  • After two days of culture in HSM, the expression levels of α-fetoprotein (AFP) and albumin, which are hepatocyte markers, were upregulated, suggesting that hepatocyte differentiation was initiated ( 19 ). (spandidos-publications.com)
  • It was hypothesized that the addition of glucose and galactose promoted hepatocyte differentiation of iPS cell. (spandidos-publications.com)
  • The HSM was modified into a hepatocyte differentiation inducer (HDI). (spandidos-publications.com)
  • Promotes differentiation of mature hepatocytes from mouse and human embryonic stem (ES) cells (Cai et al. (stemcell.com)
  • Glibenclamide uptake into hepatocytes was dramatically decreased in vivo and in vitro. (diabetesjournals.org)
  • In vivo induction of CYP1A1 in hepatocytes by aryl hydrocarbon receptor agonists is heterogeneous. (cdc.gov)
  • Combined with the reports from in vivo studies, our results support a switch with rheostat behavior for individual hepatocytes. (cdc.gov)
  • In-vivo , synthesized nanoparticles exhibited efficient dose-dependent anti-inflammatory activity reducing the dose up to 25-fold. (ac.ir)
  • Purpose: Hepatocyte growth factor (HGF), a ligand of the c-met proto-oncogene, exhibits activating effects on human lung cancer both in vitro and in vivo. (go.jp)
  • Collectively, these results suggest that binding of diclofenac to rat hepatocyte proteins is selective and that a 60-kDa microsomal membrane protein (or protein subunit) that accumulates in the plasma membrane fraction appears to be the major target for alkylation both in cultured hepatocytes exposed to diclofenac and in vivo. (aspetjournals.org)
  • GLP-1 and incretin mimetics, such as exenatide, have been shown to attenuate hepatocyte steatosis in vivo and in vitro, but the specific underlying mechanism is unclear. (diabetesjournals.org)
  • A Hepatocyte selection medium (HSM) was developed to enrich hepatocytes from co-cultures with iPS cells ( 18 ). (spandidos-publications.com)
  • Using this technology, Dr. Maher's team was able to determine the optimal conditions for culturing the liver stages of Plasmodium in primary human hepatocyte cultures. (massbio.org)
  • In general, P450 and UGT expression was lower in hepatocyte cultures than that in liver, and CYP2C9 was found to be the most abundant P450 isoform expressed in cultured hepatocytes. (aspetjournals.org)
  • Common culture formats used for in vitro enzyme induction studies comprise conventional and sandwich cultures, the former referring to plated hepatocytes maintained on rigid substratum (e.g., collagen), whereas in the latter cells are "sandwiched" by an additional layer of either collagen or a composite of extracellular matrix proteins, such as Matrigel ( LeCluyse, 2001 ). (aspetjournals.org)
  • DEHP is not classifiable as to its carcinogenicity to humans (Group 3) because peroxisome proliferation has not been documented in human hepatocyte cultures exposed to DEHP nor in the liver of exposed non-human primates. (who.int)
  • These mice are generated by transplanting human hepatocytes into albumin enhancer-driven urokinase-type plasminogen activator transgenic/severe combined immunodeficiency (uPA/SCID) mice, leading to replacement of the host hepatocytes. (wur.nl)
  • The Cu-ATPase transporter, ATP7B efficiently facilitates removal of excess Cu ions in hepatocytes. (uri.edu)
  • ATP7B together with PEG-HCuSNPs-loaded lysosomes move toward the apical domains of hepatocytes to transfer Cu into the bile canaliculi through exocytosis. (uri.edu)
  • Mutation analysis is an especially valuable diagnostic strategy for certain well-defined populations exhibiting a limited spectrum of ATP7B mutations. (medscape.com)
  • The anti-inflammatory nanoparticles manifested tumor specific release potential exhibiting selective cytotoxicity at cancerous milieu with slightly acidic environment and activated inflammatory pathways. (ac.ir)
  • Selective protein adducts to membrane proteins in cultured rat hepatocytes exposed to diclofenac: radiochemical and immunochemical analysis. (aspetjournals.org)
  • The method reported yielded synchronous populations of hepatocytes that were generated in clinically preferred conditions with minimum use of serum and cell feeders. (news-medical.net)
  • We have examined induction of CYP1A1 in vitro in primary rat hepatocytes to distinguish the responses of populations of cells and responses of individual cells. (cdc.gov)
  • Model systems to study PPARα in human liver vary from hepatoma cell lines, human primary hepatocytes, human precision cut liver slices, and mice expressing human PPARα. (wur.nl)
  • Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
  • Human embryonic stem cells also exhibit the Warburg effect ( 15 ). (spandidos-publications.com)
  • Twenty-four compounds were chosen for these studies, based on previously published data using primary human hepatocytes. (aspetjournals.org)
  • however, ability to attain data can be limited by quality and availability of human hepatocytes. (aspetjournals.org)
  • During this webinar, Dr. Steven Maher, an associate research scientist in the Center for Tropical and Emerging Global Diseases Department at the University of Georgia, will outline how his team used primary human hepatocytes to develop the first medium-throughput, 384-well plate-based liver-stage antimalarial drug discovery platform. (massbio.org)
  • They include large lots of highly viable, consistent cryopreserved human hepatocytes, and cynomolgus and rhesus hepatocytes, which have been isolated to increase their robustness and reliability in plated assays. (massbio.org)
  • Here, we report the use of a targeted liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method for concurrent quantification of multiple cytochrome P450 (P450), UDP-glucuronosyltransferase (UGT), and transporter proteins in cultured primary human hepatocytes. (aspetjournals.org)
  • The formulation displayed impressive oral bioavailability, sustained release, negligible cytotoxicity against THLE-2 normal human hepatocytes, low toxicity (high LD 50 ) and wide therapeutic window. (ac.ir)
  • After validation for correction, we will then differentiate these iPSCs into liver cells that can be transplanted into our mouse model of hemophilia that is capable of accepting human hepatocytes and allowing their proliferation. (ca.gov)
  • These mice exhibit disease symptoms similar to human patients & we propose that by injecting our corrected liver cells they will exhibit normal clotting as measured by various biochemical & physiological assays. (ca.gov)
  • We have also generated a new mouse model for the disease that is deficient in 4 genes, making it permissive to the transplantation and expansion of human hepatocytes in its liver. (ca.gov)
  • We have established a highly-efficient method for deriving hepatocytes from stem cells that mirrors events in embryonic development, " said Robert Lanza, M.D., Chief Scientific Officer at Advanced Cell Technology, Inc. and senior author of the study. (news-medical.net)
  • In this study, we showed that receptor-type PTPϵ (PTPϵM) dephosphorylated IR in rat primary hepatocytes and tyrosines 972, 1158, 1162 and 1163 were primary targets of PTPϵM. (bioone.org)
  • It was also demonstrated that PTPϵM suppressed insulin-induced glycogen synthesis and inhibited insulin-induced suppression of phosphoenol pyruvate carboxykinase (PEPCK) expression in primary hepatocytes. (bioone.org)
  • ET on March 4 that will describe how primary hepatocytes are being used to screen small molecule drugs for activity against the Plasmodium parasites that cause malaria. (massbio.org)
  • During his webinar, Dr. Maher will discuss the drug discovery platform, review complex and multiplex in vitro systems for primary hepatocyte culture, describe how to assess primary hepatocytes lots to determine their suitability for Plasmodium infection, and outline next steps in understanding the Plasmodium lifecycle. (massbio.org)
  • Induction of CYP1A1 in primary rat hepatocytes by 3,3',4,4',5-pentachlorobiphenyl: evidence for a switch circuit element. (cdc.gov)
  • Direct protein quantification was applied successfully to study in vitro induction in sandwich cultured primary hepatocytes in a 24-well format using the prototypical inducers rifampicin, omeprazole, and phenobarbital. (aspetjournals.org)
  • Mechanistic studies in liver cell lines that are confirmed to exhibit switch-like induction of single cells will be necessary to assess the molecular pathways of this circuit element. (cdc.gov)
  • Cultured hepatocytes offer the possibility to assess long-term effects, such as enzyme induction (i.e., changes of the expression levels of molecular entities of the hepatocytes). (aspetjournals.org)
  • Assay conditions, such as culture format and duration, media supplements etc., were reported previously to alter the phenotype and induction response of cultured hepatocytes ( LeCluyse, 2001 ). (aspetjournals.org)
  • Diabetes in subjects with hepatocyte nuclear factor (HNF)-1α gene mutations (maturity-onset diabetes of the young [MODY]-3) is characterized by impaired insulin secretion. (diabetesjournals.org)
  • Mutations in the hepatocyte nuclear factor (HNF)-1α gene cause the most common form of maturity-onset diabetes of the young (MODY), type 3 ( 1 ). (diabetesjournals.org)
  • In addition, hepatocytes are larger, have increased lipofuscin deposition, more frequent nuclear morphological anomalies, decreased mitochondria number, and increased mitochondrial diameter compared to wild-type mice. (nih.gov)
  • In contrast, the hepatocellular plasma membrane fraction, which also was associated with high specific binding of diclofenac-derived radioactivity by 2 hr, exhibited a approximately 3-fold increase in adduct formation by 24 hr. (aspetjournals.org)
  • The nonsteroidal anti-inflammatory drug diclofenac can be bioactivated to the reactive acyl glucuronide, which covalently binds to hepatocellular proteins in rat hepatocytes. (aspetjournals.org)
  • There were no detectable cross-reactive epitopes of proteins recognized by the antibody on Western blots of cultured hepatocytes not treated with diclofenac. (aspetjournals.org)
  • In addition to the cells ' potential use for the treatment of liver disease, hESC-derived hepatocytes could also provide a valuable model for novel pharmaceutical drug discovery assays as well as new drug metabolism and cytotoxicity screens, particularly because the liver is a major site for detoxification. (news-medical.net)
  • Troglitazone exhibited time- and concentration-dependent cytotoxicity and M-3 also exhibited weak cytotoxicity. (nih.gov)
  • Hepatocyte growth factor (HGF) is a peptide -containing multifunctional cytokine , which is overexpressed and/or activated in multiple malignancies and is reported to be associated with tumor development and inferior survival . (bvsalud.org)
  • Our results revealed that plasma HGF rather than tumor HGF exhibited a potential role in predicting metastasis and survival in SCLC. (bvsalud.org)
  • Here, we show that Wrn(∆hel/∆hel) mice exhibit increased extracellular matrix, defenestration, decreased fenestration diameter, and changes in markers of liver sinusoidal endothelial cell inflammation, consistent with age-related pseudocapilliarization. (nih.gov)
  • Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
  • We studied the function of HILDPA in adipose tissue macrophages (ATM) in the context of obesity-induced inflammation and in hepatocytes during non-alcoholic steatohepatitis (NASH). (wur.nl)
  • In the second part of this thesis, we characterized the physiological and molecular function of HILDPA in ATM in the context of obesity-induced inflammation and in hepatocytes during NASH. (wur.nl)
  • In addition to demonstrating the efficient generation of hepatocytes in research published today, the company has made significant progress in the generation of retinal pigmented epithelial (RPE) cells to treat retinal degenerative diseases and the generation of hemangioblasts to treat vascular disease as well as to create a large-scale and donorless source of red blood cells and platelets. (news-medical.net)
  • Similarly, in diet-induced NASH mice, HILPDA hepatocyte deficiency modestly yet significantly reduced liver triglyceride accumulation and plasma ALT levels. (wur.nl)
  • In vitro assays using patient muscle specimens revealed almost undetectable PFK enzyme activity, and patient blood samples exhibited erythrocyte PFK activity at about 50% of normal activity. (medscape.com)
  • Using a solid methodology that includes state-of-the-art microscopy, proteome arrays, and gene ontology assays, it provides strong evidence that liver-derived IgG and IgA with cytotoxic properties and reactivity to both gut-derived E.coli and autoantigens accumulated in hepatocytes of SAH patients but not of healthy controls. (elifesciences.org)
  • Lactate may be applied to produce hepatocytes from iPS cells more efficiently. (spandidos-publications.com)
  • These methods have been previously used to obtain hepatocytes from iPS cells. (spandidos-publications.com)
  • Advanced Cell Technology, Inc. has reported for the first time a robust and highly efficient process for the generation of high-purity hepatocytes (liver cells). (news-medical.net)
  • The research, described online (ahead of print) in the journal STEM CELLS , signifies a significant step towards the efficient generation of hepatocytes for use in regenerative medicine and drug discovery. (news-medical.net)
  • In an animal model of acute liver injury, the hESC-DE cells differentiated into hepatocytes and successfully repopulated the damaged liver. (news-medical.net)
  • Immortalized hepatocytes (Fa2N-4 cells) have been proposed as a tool to identify CYP3A4 inducers. (aspetjournals.org)
  • Microarray data revealed that the Wrn(∆hel/∆hel) genotype does not affect the expression of many genes within the isolated hepatocytes or liver sinusoidal endothelial cells. (nih.gov)
  • Direct conversion from fibroblasts to generate hepatocyte like-cells (iHeps) bypassing the pluripotent state has been described in previous reports as an attractive method acquiring hepatocytes for cell-based therapy. (figshare.com)
  • The pharmacological properties of herbal remedies, including the amelioration of insulin sensitivity, the promotion of insulin secretion, the increase of glucose uptake by adipose and muscle tissues, the inhibition of sugar absorption in intestines and the generation of sugar by hepatocytes, have been demonstrated in several studies ( 15 , 16 ). (spandidos-publications.com)
  • This hemophilic mousewill allow us to screen for symptoms of disease alleviation upon administration of patient derived hepatocytes. (ca.gov)
  • Patients with the hemolytic form of Tarui disease do not present with muscle symptoms but rather exhibit nonspherocytic hemolytic anemia. (medscape.com)
  • As these hepatocytes can mimic liver properties, they allow his team to evaluate the metabolism, drug-drug interaction, and toxicity of drug candidates in addition to their impact on parasite load. (massbio.org)
  • Short term cultured rat hepatocytes were used to further study the formation and nature of protein adducts after exposure to diclofenac. (aspetjournals.org)
  • Large scale production of hepatocytes using this method should greatly bolster their applications in basic research, clinical medicine and preclinical drug discovery. (news-medical.net)
  • To this end we used a HILPDA tissue-specific knockout mice model in macrophages and hepatocytes generated by using LysM-Cre and Alb-Cre transgenic mice, respectively. (wur.nl)
  • These results suggest that troglitazone induces apoptotic hepatocyte death which it may be one of the factors of liver injury in humans. (nih.gov)
  • The levels of metabolizing enzymes and transporters expressed in hepatocytes are decisive factors for hepatobiliary disposition of most drugs. (aspetjournals.org)