Evidence bacterial isolation microbial viability. Medical search. Frequent questions

Nucleic acid amplification diagnostics offer a rapid and accurate method of diagnosing microbial pathogens. (medscape.com)
It has been used for the diagnosis of multiple viral and bacterial pathogens, including H influenzae . (medscape.com)
Thus, allows the recovery of new bacterial species from heavily virulent capsular strains were phagocytosed by and repli- contaminated samples and might be a valuable approach cated in Acanthamoeba castellanii , leading to amoebal for the recovery of as-yet unrecognized emerging death, while the nonvirulent acapsular strains were killed pathogens from clinical specimens. (cdc.gov)
In other bacterial pathogens, genes important for pathogenicity and host range include secreted protein effectors that suppress host immunity, alter host metabolism, and enable colonization, providing a fitness advantage. (biorxiv.org)
The majority of acute exacerbations of COPD (AECOPD) is associated with an acute respiratory infection, with viral and/or bacterial pathogens being detected in more than half of all exacerbations [ 1 , 2 ]. (biomedcentral.com)
AECOPD associated with the detection of a combination of bacterial and viral pathogens have been reported to be particularly severe in terms of inflammation and decline in lung function [ 20 ]. (biomedcentral.com)

Evidence for the Role of Salicylic Acid in Thermoregulation in Sporophylls of Male Cones of Six Cycad Species. (montgomerybotanical.org)
Based on these data, we conclude that this isolate represents a new bacterial species belonging to the family Cellulomonadaceae and the phylum Actinomycetota. (bvsalud.org)
Polymicrobial infections involve both detrimental and beneficial interactions between different bacterial species. (biomedcentral.com)
Co-infection by different pathogenic bacterial species often increases the severity of the underlying condition, and are more difficult to treat [ 1 ]. (biomedcentral.com)
Antibiosis, which is defined by detrimental interactions between at least two species, usually involves the synthesis of antimicrobial molecules that inhibit growth or kill other bacterial species [ 18 ]. (biomedcentral.com)
Among the bacterial species that are frequently detected during such episodes are strains of non-typeable (NT) Haemophilus influenzae , Pseudomonas aeruginosa and Streptococcus pneumoniae [ 1 ]. (biomedcentral.com)

Despite possessing many commercially attractive properties and clear advantages compared with their synthetic counterparts, the production of microbial surfactants on a commercial scale has not been realized because of their high production costs. (scialert.net)

The peptic ulcer (gastric and duodenal) using strongest evidence for the pathogenic role endoscopic examination at the endoscopic of H. pylori in peptic ulcer disease comes unit, Basra General Hospital, Iraq, by a spe- from treatment trials. (who.int)

Chemical interaction with matrix molecules is hypothesized, based on SEM images and considering the low or absent anti-microbial activity on planktonic bacteria showed by flow cytometry. (frontiersin.org)
Here, we review the literature on culture-independent analysis of microbial diversity in the BE and explore what it tells us about the dynamics and distribution of bacteria and fungi in these environments. (biomedcentral.com)
The role of bacterial exopolysacchairdes in the maintenance of healthy human gut bacteria. (nld-dtp.org.uk)
After biomechanical preparation of root canals, the bacterial load is reduced by at least 95% and the remaining viable bacteria may be the cause of refractory lesions 8 . (bvsalud.org)
Often starting as a phagosome in the endocytic compartment, these bacteria-occupied vacuoles transition into organelles with novel molecular features via remodeling of host proteins and lipids through the highly coordinated actions of bacterial factors [3] [5] . (microbialcell.com)

Culture-based investigations of a small number of bacterial taxa have indicated that exposure to light, and especially ultraviolet (UV) wavelengths [ 16 , 22 - 25 ], can inactivate many microorganisms and therefore potentially reduce dust microbial community viability. (biomedcentral.com)
A coherent understanding of when mortality of viable microorganisms does or does not occur in dust, and whether different light exposures influence these processes at the microbial community scale, is still lacking. (biomedcentral.com)

It is envisaged that these dual-functional materials will be capable of inhibiting biofilm formation when deployed as a coating on medical devices, protecting patients from bacterial infections, while simultaneously providing visual feedback to healthcare professionals if the material has reached the end of its anti-infective lifespan. (qub.ac.uk)
Infections with group B Streptococcus, Listeria monocytogenes, or gram-negative rods (eg, Escherichia coli, Klebsiella pneumoniae) are common causes of bacterial pneumonia. (medscape.com)
Several BS exhibit anti-bacterial, anti-fungal and anti-viral activities, making them appropriate candidates to combat infections [ 3 ]. (biomedcentral.com)
In cases of primary endodontic infections, there is a higher bacterial load than in secondary infections 6-7 . (bvsalud.org)
Next to viral infection, also bacterial infections can be involved in the development of acute exacerbations. (biomedcentral.com)
These new antimicrobial and antibiofilm technologies will treat wound and medical device related infections, and will provide an alternative treatment to antibiotic classes in which bacterial resistance is high. (cdc.gov)

Using bacterial cultivation methods, bacterial isolates belonging to the genus Enterococcus were identified. (bvsalud.org)

Emerging evidence suggests that the host can sense gut bacterial metabolites in addition to pathogen-associated molecular patterns and that recognition of these small molecules influences host immune response in the intestines and beyond. (nature.com)
Microbial Community and Potential Pathogen Shifts Along an Ornamental Fish Supply Chain. (mbl.edu)
Streptococcus pneumoniae is the most common bacterial pathogen in infants aged 1-3 months. (medscape.com)
Legionella pneumophila is an accidental human bacterial pathogen that infects and replicates within alveolar macrophages causing a severe atypical pneumonia known as Legionnaires' disease. (microbialcell.com)

As a result, R. chinensis is a good candidate to examine for in vitro anti-bacterial and anti-proliferative properties. (biomedcentral.com)
Anti-Bacterial Agents. (bvsalud.org)

To test the first possibility, we measured the effect of UA on bacterial metabolism by measuring the growth of two Escherichia coli strains, OP50 and HT115, after treatment with UA. (lsd1receptor.com)

ABSTRACT The study aimed to develop a rapid and simple method for the primary isolation and de- tection of Helicobacter pylori from dyspeptic patients. (who.int)

One synthetic pathway produced valine at a sufficient level for cell viability and proliferation. (elifesciences.org)
TLR4 absence can lead to severe mucosal damage from a loss in epithelial proliferation, impaired inflammatory response, and bacterial translocation. (biomedcentral.com)

Since the recent advent of culture-independent analyses of complete microbial populations, microbe-host-microbiota interactions have opened new avenues for the study of infectious diseases. (biomedcentral.com)
Therefore, it plays an essential biological role in the control of bacterial populations. (hindawi.com)

This project aims to develop new carbohydrate-functionalised polymeric surfaces which will inhibit bacterial adhesion. (qub.ac.uk)

Several studies have examined the independent influence of each control strategy in mitigating transmission in isolation, yet controls are often implemented concomitantly within an indoor environment. (cdc.gov)
Microbial communities associated with indoor dust abound in the built environment. (biomedcentral.com)

Identifying novel antimicrobial agents from plant-based compounds may result in anti-microbial drugs effective against the resistant strains. (biomedcentral.com)
Infection with influenza B on the other hand did not affect cytokine production in BEAS-2B cells exposed to the different bacterial strains. (biomedcentral.com)

An increasing number of studies have provided strong evidence that gut microbiota interact with the immune system and stimulate various mechanisms involved in the pathogenesis of auto-immune diseases such as Systemic Lupus Erythematosus (SLE). (bvsalud.org)
Therefore, the aim of this present study was to describe the bacterial component of the microbiota of ballan wrasse eggs shortly after spawning and at 5 days, once the eggs had undergone a routine incubation protocol that included surface disinfection steps in a common holding tank. (omicsdi.org)
This first comprehensive description of the ballan wrasse egg bacterial community is an initial step to understand the role and function of the microbiota on the phenotype of this fish. (omicsdi.org)

In man-made water systems microbial biofilms increase the resistance of legionella to disinfection, posing a significant threat to public health. (frontiersin.org)
Bacterial ability to form biofilms was verified using a crystal violet colorimetric assay and testing cell viability by real-time quantitative PCR and Plate Count assay. (frontiersin.org)
Biofilms are conglomerations of bacterial cells protected by self-synthesized extracellular polysaccharide matrices (EPS). (biomedcentral.com)

The viability of H influenzae is lost rapidly, so clinical specimens should be inoculated to an appropriate culture media without delay. (medscape.com)
For bacterial culture, specimens were cultured in parallel on 2 media: the non- selective classic Columbia agar and the selective modified Columbia urea agar (MCUA). (who.int)

Here we investigated the link between cellular cholesterol and L. pneumophila intracellular replication and discovered that disruption of cholesterol biosynthesis or cholesterol trafficking lowered bacterial replication in infected cells. (microbialcell.com)

Effects of synonymous mutations beyond codon bias: the evidence for adaptive synonymous substitutions from microbial evolution experiments. (qascf.com)
IL-8) and chemokines that recruit innate and adaptive immune cells to limit bacterial invasion. (biomedcentral.com)

Specifically, we placed household dust in replicate model "rooms" with windows that transmitted visible, ultraviolet, or no light and measured taxonomic compositions, absolute abundances, and viabilities of the resulting bacterial communities. (biomedcentral.com)

In Chile, there are yet no studies about the presence of this bacterial genus in CAP. (bvsalud.org)

Based on the significant antibacterial activity, the sorting of bacterial cells against the compounds (MID6 and MID9) was analyzed by flow cytometry. (degruyter.com)

The environmental sources of microbial aerosols and processes by which they are emitted into the atmosphere are not well characterized. (nature.com)

13 A role for microbial signals via Toll-like receptors (TLRs) has been reported in mediating intestinal epithelial cell (IEC) and dendritic cell (DC) induction of the production of IgA through the induction of BAFF and APRIL. (nature.com)
A significant decrease (0.05-0.5 μM) was observed in the cell viability and IC 50 dosage was fixed as 0.25 μM after 24 h of treatment. (degruyter.com)
To determine the safety of the fermented extracts, their cytotoxicity was analyzed by measuring the cell viability of CCD-966SK and human epidermal melanocytes (HEMn) after exposure. (hindawi.com)

Test results on body fluids from various sites of infection that reveal small, gram-negative, pleomorphic coccobacilli with polymorphonuclear cells are strong evidence of infection. (medscape.com)

Altogether, this work was found to be of substantial interest as it provides pioneering evidence that mammalian systems may be permissive to the restoration of essential amino acid biosynthetic pathways and is thus anticipated to have a broad impact in the fields of synthetic biology, biotechnology and beyond. (elifesciences.org)
The TLR signaling pathways help to maintain a constant homeostatic regulation of microbial load and the immune response. (biomedcentral.com)

INTRODUCTION: Breath testing has become a widely used tool to diagnose small intestinal bacterial overgrowths (SIBOs) and intestinal methanogen overgrowths (IMOs) in clinical settings. (bvsalud.org)
We isolated fecal content and small intestinal leukocytes from murine, Roux-en-Y and end-to-end anastomoses, to identify microbiome changes and subsequent alterations in the regulatory and pro-inflammatory immune cells 3 days post-operative. (biomedcentral.com)

2 methods: histopathological examination tion is focal, negative biopsy results do not and bacterial culture growth. (who.int)
This approach readily determines several orders of magnitude more microbial diversity than culture-based methods, often discovering novel organisms with no known growth conditions, and has dramatically altered our understanding of global microbial diversity [ 14 ]. (biomedcentral.com)
I will use growth studies, microbial community profiling, recombinant protein technology and biochemical analysis of degradation products and enzyme activity to elucidate the carbohydrate mediated relationship in the complex environment that is the human gut. (nld-dtp.org.uk)
Fasting can be induced if a compound affects bacterial growth, thereby reducing the amount of food available or modifying key nutrient amounts14, or acts as repellent for the worms and prevents food intake. (lsd1receptor.com)
After 24 h of incubation, MIC was determined as the lowest concentration capable of inhibiting microbial growth, identified by the resazurin reaction (100 µg/mL). (bvsalud.org)
Conversely, bacterial growth within cholesterol-leaden macrophages was enhanced. (microbialcell.com)

There is little information available regarding the bacterial community structure in commercial zebrafish housing systems, or the time-point at which the system or biofilter reaches a microbiological equilibrium in RAS in general. (biomedcentral.com)

Such bacterial presence may lead to clinical symptoms of persistent chronic apical periodontitis (CAP), which has high prevalence in some countries, ranging from 40% to 61% 3-4 . (bvsalud.org)

Right lower lobe consolidation in a patient with bacterial pneumonia. (medscape.com)

During retreatment, a microbial sample was obtained from the root canal and inoculated in a selective Enterococcus medium. (bvsalud.org)

His research interest is in drug discovery involving extraction, isolation, characterization and bioactivity study of natural products from plants. (iium.edu.my)

In this study we analyzed microbial cells and biological ice nucleating particles (INPs) in smoke emitted from eight prescribed wildland fires in North Florida. (nature.com)
When compared to air sampled prior to ignition, samples of the air-smoke mixtures contained fivefold higher concentrations of microbial cells (6.7 ± 1.3 × 10 4 cells m −3 ) and biological INPs (2.4 ± 0.91 × 10 3 INPs m −3 active at temperatures ≥ −15 °C), and these data significantly positively correlated with PM 10 . (nature.com)
From these data, we estimate each fire aerosolized an average of 7 ± 4 × 10 9 cells and 2 ± 1 × 10 8 biological INPs per m 2 burned and conclude that emissions from wildland fire are sources of viable microbial aerosols to the atmosphere. (nature.com)

This study provides direct evidence of human impact on the make- up of NOM: the cultures of microbes found at a WWTP consume the proteinaceous material, whereas humic-like and fulvic-like materials are produced. (scirp.org)
Thus far, specific SLE microbial signatures have not yet been clearly identified with alteration patterns that may vary between human and animal studies. (bvsalud.org)

Identified lack of evidence of methanogens in Mallik 5L-38 samples, and identified molecular evidence of methanogens in Leg 204 corehole. (doe.gov)
The most commonly used molecular approach for exploring microbial diversity is based on the PCR amplification and sequencing of genes encoding small subunit ribosomal RNA (16S rRNA) directly from environmental sources [ 16 ]. (biomedcentral.com)

Scientific evidence of the pharmacological activity of the plant strengthens the traditional application of plants. (biomedcentral.com)

MCUA showed a higher isolation rate than classic Columbia agar (67.6% versus 44.1% of patients), and the results were obtained faster (24 hours versus 5-7 days) with more clear-cut identification. (who.int)

Several breakthrough points are highlighted concerning the three adopted comprehensive self-resilience systems, namely physical, chemical and microbial systems. (mdpi.com)

Microbial viability of smoke aerosols based on formazan production and epifluorescent microscopy revealed no significant difference in the viable fraction (~80%) when compared to samples of ambient air. (nature.com)
From within a unique ER-derived vacuole L. pneumophila promotes host lipogenesis and experimental evidence indicates that cholesterol production might be one facet of this response. (microbialcell.com)

Concernant la culture bactérienne, les échantillons ont été mis en culture en parallèle dans deux milieux : la gélose Columbia classique non sélective et la gélose Columbia sélective modifiée à l'urée. (who.int)

There is an enormous amount of scientific evidence available on this topic. (substack.com)
For biofilm control in patients at risk of caries, educational and therapeutic actions such as supervised brushing and topical application of fluoride are particularly encouraged in communities with limited access to health services, but there is little scientific evidence that these interventions may reduce dental caries in children [4,5]. (bvsalud.org)

Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery. (omicsdi.org)
Bacterial communities of eggs varied more greatly between tanks than between samples deriving from the same tank. (omicsdi.org)

Des échantillons de muqueuse ont été prélevés grâce à une biopsie antrale sur 136 patients dyspeptiques consécutifs pour lesquels un diagnostic d'ulcère gastro-duodénal avait été établi par endoscopie à l'hôpital général de Bassora (Basra) en Iraq. (who.int)
Since the isolation of Helicobacter tion of H. pylori from dyspeptic patients. (who.int)

Anatomy4

Organisms6

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Psychiatry and Psychology1

Phenomena and Processes32

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care3

Pathogens6

Species6

Surfactants1

Pathogenic1

Bacteria5

Microorganisms2

Infections6

Isolates1

Pathogen4

Anti-bacterial2

Escherichia1

Abstract1

Proliferation2

Populations2

Inhibit1

Indoor2

Strains2

Microbiota3

Biofilms3

Specimens2

Biosynthesis1

Adaptive2

Abundances1

Genus1

Compounds1

Processes1

Cell3

Infection1

Pathways2

Intestinal2

Growth6

Community1

Clinical1

Pneumonia1

Selective1

Extraction1

Biological3

Human2

Molecular2

Activity1

Higher1

Systems1

Production2

Culture1

Scientific2

Communities2

Patients2