Evaluate cytotoxicity drug screening assays antitumor. Medical search. Frequent questions

The same extracts were assessed for their antioxidative potentials with the use of DPPH free radical scavenging assay followed by determination of HRBC membrane stabilization method, Brine Shrimp Lethality Assay (BSLA) and GC-MS analysis. (biomedcentral.com)
During Brine Shrimp Lethality Assay, all extracts of BG were found to be bioactive and the degree of lethality was found to be concentration dependent. (biomedcentral.com)
The Brine Shrimp Lethality Assay found extracts to be bioactive suggesting extracts as a promising candidate for plant-derived anti-tumor compounds. (biomedcentral.com)
The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)

Kroll A(1), Pillukat MH, Hahn D, Schnekenburger J. The in vitro assays covered baseline toxicity in bacteria, oxidative stress, cytotoxicity in mammalian cells and yeast, estrogenicity, and anti-androgenicity. (holocausto.online)
H … In vitro assays covered baseline toxicity, oxidative stress responses, and estrogenic and antiandrogenic activity. (holocausto.online)
In line with these observations, the allogeneic CAR T cells showed superior cytotoxicity in in vitro assays, and they efficiently targeted primary multiple myeloma cells in an ex vivo model. (liangyihui.net)

To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)
The phytochemical screening detected the presence of saponins, alkaloids, reducing sugars and coumarins. (researchgate.net)
Phytochemical screening test revealed the presence of terpenoid, flavonoid, tannin, saponin and phenolic compound in all the stem bark extracts of C. odontophyllum. (scirp.org)
Ziziphus spina-christi (L.), commonly called jujube, was evaluated for their phytochemical content in the stem bark as well as the antimicrobial and cytotoxic activities. (biomedres.info)
Both ethyl acetate extract and alkaline ethyl acetate extract were tested for phytochemical content by qualitative analysis and evaluated for their biological activities such as antimicrobial (by agar well diffusion assay and minimum inhibitory concentration determination) and cytotoxic activities (by MTT assay in cell culture). (biomedres.info)

Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. (nature.com)
Potent cell growth inhibitory properties were shown by ligands MS47 and MS49 against human melanoma MDA-MB-435, colon cancer HCT-116 and COLO 205, and pancreatic cancer MIA PaCa-2 cell lines, as evidenced by MTT assay. (openmedicinalchemistryjournal.com)
NK cells are specialized effectors of the innate immune system that destroy their targets by antibody-dependent cell-mediated cytotoxicity, have prominent antitumor effects, and are potent killers of virally infected cells. (medscape.com)

The inclusion of other assays and compounds will most likely expand the types of activities that can be reliably detected. (holocausto.online)
Traditionally, the in vitro toxicity assays that have been used to identify molecules that carry drug-related DILI risk factors include two-dimensional (2D) cell systems and other standard preclinical model… The principal application of this assay is to rapidly screen test compounds to identify those that induce acute liver cell toxicity. (holocausto.online)
Studies have found very good relationship between this simple, inexpensive, and bench-top assay and the antitumor potential of the cytotoxic compounds [ 10 ]. (biomedcentral.com)
Using a molecular docking approach and the National Cancer Institute (NCI) compound dataset, eight candidate compounds were selected, and we evaluated their efficacy. (oncotarget.com)
Cytotoxicity and anticancer potential of newly synthetisized compounds were investigated. (japsonline.com)
The antiproliferative effect of compounds was determined by WST-1 test, and nuclear morphology changes in cancer cells stained with Giemsa were evaluated. (japsonline.com)
The present work focused on (1) synthesis of β-amino- and β-iminophosphonates via a nucleophilic addition reaction of benzylamine and 2-aminopirydine to 1,2-alkadienephosphonates, i.e., dialkyl esters and amidoesters, as well as dimethyl- and diphenylphosphine oxides, and (2) investigation of cytotoxicity and anticancer potential of synthesized compounds. (japsonline.com)

The active compound curcumin is believed to have a wide range of biological effects including anti-inflammatory, antioxidant, antitumor, antibacterial, and antiviral activities, which have shown a lot of potential for use in clinical medicine. (blogspot.com)

Microtubule binding drugs are of special interest as they have important roles in the modulation of cellular functions and many of them act as anticancer agents. (rgcb.res.in)
SOR), hydrogen peroxide, and 2, 2-Diphenyl-2-picrylhydrazyl (DPPH) radical activity by a model (most biological y active) of the anticancer plant was also evaluated. (who.int)
L. egeregia was regarded as the prototype of the anticancer species due to its profound flavonoid concentration (85.40 µg/mL) and cytotoxicity (9.46 µg/mL) compared to other extracts. (who.int)
Further animal model studies, toxicity assays and pharmacological studies of this asparaginase from the endophyte would help to authenticate the use of this enzyme as an anticancer drug. (greenpharmacy.info)

The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)

On the basis of the above results 7 herbs were further screened in order to test their efficacy against targeted mycotoxins: Glycyrrhiza glabra, Tinospora cordifolia, Zingiber officinale, Curcuma longa, Centella asiatica, Silybum marianum, Withania somnifera. (europa.eu)
Integration of a machine learning-derived signature of response with in vitro assessment of LP-184 efficacy facilitated the derivation of manageable yet robust biomarkers which can be used to predict drug sensitivity with high accuracy and clinical value. (biomedcentral.com)
In a humanized mouse non-small cell lung carcinoma model, TSR-033 boosted the antitumor efficacy of PD-1 monotherapy, with a concomitant increase in immune activation. (aacrjournals.org)

Tumor-specific neoantigens, which are expressed on tumor cells, can induce an effective antitumor cytotoxic T-cell response and mediate tumor regression. (wjgnet.com)
LN metastases resist T cell-mediated cytotoxicity, induce antigen-specific regulatory T cells, and generate tumor-specific immune tolerance that subsequently facilitates distant tumor colonization. (stanford.edu)
When loaded with immune complexes (IC), consisting of tumor antigens bound to antitumor antibody, BMDC induce powerful antitumor immunity in mice. (stanford.edu)

The objective was to define the mechanism of the growth inhibition of active hexose correlated compound (AHCC) alone and evaluate its activity in combination with pegylated liposomal doxorubicin (PLD). (researchgate.net)
Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
Major Findings: The in vitro growth inhibition assays demonstrated additive activity when AHCC is co-administered with PLD. (researchgate.net)
Triticum aestivum L. root growth inhibition test and Allium cepa L. assay were used to assess the cytotoxic potential of phosphonates. (japsonline.com)

The preliminary study is aimed at evaluating the cytotoxic activity of crude extracts from the stem bark of Canarium odontophyllum against colorectal carcinoma cell HCT 116. (scirp.org)
The pharmacological screening studies indicated that the aqueous extract of Z. spina-christi root bark has an antinociceptive activity in mice and rats [ 23 ] and a central depressant effect in mice [ 24 ]. (biomedres.info)
The interaction of human topoisomerase 1B and dimethylmyricacene, a compound prepared semisynthetically from myricanol extracted from Myrica cerifera root bark, was investigated using enzymatic activity assays and molecular docking procedures. (mdpi.com)

Prediction of Chemical Carcinogenicity in Rodents from in Vitro Genetic Toxicity Assays R AYMOND W. TENNANT, BARRY H. MARGOLIN, MICHAEL D. SHELBY, E RROL Z … As an in vivo toxicity endpoint, the acute lung inflammogenicity in a rat instillation model was compared with the in vitro toxicity endpoints comprising cytotoxicity, pro-inflammatory cytokine expression, or haemolytic potential. (holocausto.online)
In vitro toxicity assays are used to determine the potential of a new pharmaceutical, agrochemical, food additive, or other chemical product to be hazardous to humans.In vitro studies, which are performed on cultured bacterial or mammalian cells, can be used as an initial screen to avoid the unnecessary use of animals in determining which candidates should move forward for further safety testing. (holocausto.online)
The application of High Content Screening for in vitro toxicity testing is a relatively new approach in the preclinical research phase of drug development. (holocausto.online)
In regulatory testing situation these in vitro toxicity assays must be reliable, because from these tests it is only possible to generalize the data to … First Published 1995. (holocausto.online)
While toxicity test has been traditionally completed in the preclinical phase, in vitro toxicity studies in the early drug discovery stages could significantly reduce failures at a later stage and prevent economic loss. (holocausto.online)
Considering the ethical issues and the cost of in-vivo animal tests, the pharmaceutical industry now relies more on in-vitro methods for toxicity testing in the drug development phase. (holocausto.online)
The positive control induced a high degree of toxicity in all in vitro tests performed, indicating that the toxicity observed in the direct contact assay was due to in situ extraction of toxic components. (holocausto.online)
This assay has been extensively used for different studies such as for preliminary toxicity screening of plant extracts, detection of fungal toxins, plant extract toxicity, heavy metals, cyanobacteria toxins, pesticides, and cytotoxicity testing of dental materials [ 9 ]. (biomedcentral.com)

Chemotherapeutic drugs target cancer-specific characteristics, such as alterations in DNA repair, which has complicated roles in oncogenesis and tumor progression. (biomedcentral.com)
Cyclophosphamide (CTX) is a commonly used alkylating anti-tumor drug. (peroxidasessignaling.com)
On the contrary, small size NPs with a diameter of 4-20 nm easily penetrate into deep tumor tissues, but they are more prone to rapid clearance and insufficient drug retention. (nature.com)

Their current application in oncology has revolutionized cancer treatment by improving the antitumor activity of several chemotherapeutic agents. (mdpi.com)
An MTT assay was used to determine cell metabolic activity and a TUNEL assay for detecting DNA fragmentation. (holocausto.online)
NSAIDS are widely used for their anti-inflammatory, analgesic and antipyretic activity and are among the most widely used drugs worldwide [ 6 ]. (biomedcentral.com)
alone and evaluate its activity in combinatio n with pegylated liposomal doxorubicin (PLD). (researchgate.net)
We provide experimental evidence demonstrating that ebastine has antitumor activity in osteosarcoma and promotes autophagy by activating the AMPK/ULK1 signaling pathway, which is IPMK dependent. (ijbs.com)
Pharmacophore-based activity profiling screen demonstrated some biological targets that MS44-53 may modulate their biological response, and thus can be considered as potential drugs to treat different kinds of diseases, such as carcinoma, diabetes type II, bacterial infection and cardiovascular diseases. (openmedicinalchemistryjournal.com)
Introduction: L-Asparaginase belonging to a group of amidase possesses a broad spectrum of antitumor activity. (greenpharmacy.info)
We focused on disease activity and joint damage, evaluated on radiographs, as end result variables. (exposed-skin-care.net)

30 microg/ml) that are comparable with well-established chemotherapeutic drug, 5-fluorouracil. (unboundmedicine.com)

Objective: In the present study, L-asparaginase producing endophytic fungi were isolated from Justicia adhatoda and cytotoxicity studied on A549 cell line. (greenpharmacy.info)
MTT assay and dual staining with acridine orange/ethidium bromide were done to assess the type of cell death induced by the enzyme in A549 cells. (greenpharmacy.info)

We conduct cytotoxicity estimation using a panel of cell proliferation and viability tests, which can be sorted into four types by different endpoints: Functional assays: assessment of ATP, ADP, ATP/ADP ratios. (holocausto.online)
Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)

These include EpiScreen™, a T-cell epitope mapping technology that screens peptides for their immunostimulatory properties, and EpiScreen DC:T cell assay, which screens biologics for their immunogenicity. (genengnews.com)

In vitro, chemicals such as drugs and pesticides have different cytotoxicity mechanisms such as destruction of cell membranes, prevention of protein synthesis, irreversible binding to receptors etc. (holocausto.online)

Overall, our study offers a novel conceptual framework for biomimetic NPs using a DoE strategy and consolidates the high therapeutic potential of leukosomes as a viable drug delivery system for anti-inflammatory and antineoplastic applications. (frontiersin.org)
Epidemiological and clinical studies have suggested that cancer could be prevented or significantly reduced by treatment with anti-oxidant and anti-inflammatory drugs, therefore, curcumin, a principal component of turmeric (a curry spice) showing strong anti-oxidant and anti-inflammatory activities, might be a potential candidate for the prevention and/or treatment of cancer and other chronic diseases. (blogspot.com)

Cytology/Membrane leakage assays: LDH, AK test. (holocausto.online)
Specifically, the use of leukocyte-derived membrane proteins to functionalize nanovesicles (leukosomes) can enable their long circulation and target the inflamed endothelium present in many inflammatory pathologies and tumors, making them a promising and versatile drug delivery system. (frontiersin.org)

Proliferation assays However, in rats just minimal alterations were noted. (holocausto.online)

While the epitope of most B7-H3 antibodies has not been described, one of the clinically tested antitumor antibodies (8H9) was found to bind to the IgV domain 19 . (nature.com)
The present study is usually a cross-sectional analysis aimed to evaluate the significance of the presence of SE genes, defined as em DRB1*01 /em or em DRB1*04 /em , in relation to anti-CCP antibodies, antikeratin antibody (AKA) and RF in individuals who developed RA. (exposed-skin-care.net)

The antioxidant capability of the resulting plant extracts against a stable radical DPPH was evaluated and the most of plant extracts showed high efficiency in the DPPH test. (europa.eu)

Open data on the gene expression of the NCI-60 cell lines, provides a unique opportunity to add another dimension to the preclinical development of such drugs by interrogating correlations with gene expression patterns. (biomedcentral.com)

Mammalian genotoxicity assay reveals if the compound causes abnormalities in chromosome distribution (aneugenity) or chromosome breaks (clastogenity) during cell division. (holocausto.online)
Here, we first used high-throughput screening and had screened one compound candidate, ebastine (a H1-histamine receptor antagonist), for osteosarcoma therapy. (ijbs.com)

Smartly engineered nanocapsules can not only shrink and decompose into small-sized nanodrugs upon drug release but also can regulate the TME to overproduce reactive oxygen species for enhanced synergistic therapy in tumors. (nature.com)

Tulp, M. and Bohlin, L. (2002) Functional versus Chemical Diversity: Is Biodiversity Important for Drug Discovery? (scirp.org)
Functional disability was evaluated using health assessment questionnaire [7]. (exposed-skin-care.net)
We have generated a high affinity and selective humanized monoclonal IgG4 antibody, TSR-033, which binds human LAG-3 and serves as a functional antagonist, enhancing in vitro T-cell activation both in mixed lymphocyte reactions and staphylococcal enterotoxin B-driven stimulation assays. (aacrjournals.org)

Typically, low molecular weight drugs are not recognized by the immune system, whereas protein molecules of much higher molecular weight invariably are. (genengnews.com)
2011). Given the cytotoxicity of CTX immune cells (Gilman, 1963), it has been widely used to establish immunosup- pressant model in mammals (El-Abasy et al. (peroxidasessignaling.com)

Extraction and direct contact assays were performed using human epidermal keratinocytes and mouse fibroblasts and mouse epithelial cells. (holocausto.online)
The development of biomimetic nanoparticles (NPs) has revolutionized the concept of nanomedicine by offering a completely new set of biocompatible materials to formulate innovative drug delivery systems capable of imitating the behavior of cells. (frontiersin.org)
This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
The first-in-human testing of scFv-based B7-H3 CAR-T cells has shown antitumor response against anaplastic meningioma and childhood diffuse intrinsic pontine glioma without severe side effects 16 , 17 . (nature.com)
The addition of a gamma secretase inhibitor, which targets an enzyme that cleaves BCMA from multiple myeloma cells, enhanced the cytotoxicity of allogeneic CAR T cells. (liangyihui.net)
Then the in vivo sensitivity of these transfected C6-TK and C6-sr39TK cells to GCV was determined by 3-(4,5)-dimethylthiahiazo-(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) colorimetric assay and Hoechst-propidium iodide (PI) staining. (hindawi.com)

The use of nanoparticle (NP) delivery systems in cancer treatment has received significant interest, however use of such systems in delivery of cytotoxic chemotherapy agents can be limited by low encapsulation efficiency and burst release of the cytotoxin, as well issues with throughput and reproducibility during the fabrication of drug-loaded NPs. (bvsalud.org)
A clinical study to evaluate of the combination of AHCC plus PLD in the treatment of ovarian cancer is being pursued. (researchgate.net)
These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)
The Part 2 post in this series is concerned with mainline anti-cancer drug interventions that simultaneously address multiple growth pathways, ones that are entering clinical practice. (anti-agingfirewalls.com)
In particular, the discovery of the new use of artemisinin derivatives as excellent anti-cancer drugs is also reviewed. (anti-agingfirewalls.com)
Cancer AND drug name. (clinicaltrialsregister.eu)
Norwati, D., Harmy, M.Y., Mohd Noor, N. and Amry, A.R. (2014) Colorectal Cancer Screening Practices of Primary Care Providers: Results of a National Survey in Malaysia. (scirp.org)
Smart drug delivery systems (SDDSs) for cancer treatment are of considerable interest in the field of theranostics. (nature.com)
and many others - see Advanced Drug Delivery Systems of Curcumin for Cancer Chemoprevention for a good overview. (blogspot.com)

The rationale of these traditional uses in African traditional medicine was established by screening several species for biological activities. (scialert.net)

With these partial structures established, their connectivity was evaluated via HMBC spectroscopy, a 2D NMR technique which allows for the determination of heteronuclear J-coupling values for nonadjacent carbons and protons. (wikipedia.org)
These methods use NMR to evaluate the spin-spin coupling constants which directly relate to the dihedral angle of the atoms being analyzed, allowing for the determination of chirality. (wikipedia.org)

In this study, the researchers retrospectively calculated polygenic risk scores (PRS)-estimates of disease risk based on the presence of multiple genetic variants-for 12,609 individuals enrolled in the ASPREE clinical trial, which evaluated the potential health benefits of daily aspirin among older adults. (liangyihui.net)

Substantial reductions will be anticipated to require at least two modifications at nonproximal residues?a forecast consistent with findings from our mutagenesis screen, though mutations that destabilize the inactive conformation of ABL to which AP24534 binds, including T315I and E255V, lead to small reductions in binding affinity. (others-signal.com)

Because we showed that integration neither affects the levels of viral genes, nor those of virally disrupted human genes, a genome-wide screen was performed to identify human genes which expression is influenced by viral integration and have clinical relevance. (cancerindex.org)

It shows cytotoxicity with GI50 values ranging from 0.05 to 1 μm in different malignant cell lines with an average value of 0.35 μm. (rgcb.res.in)
Flow cytometry analysis was performed to evaluate the cell cycle arrest phase induced by different concentration of partially purified sample (80 Î¼g/ml and 160 Î¼g/ml). (greenpharmacy.info)

2019). Thus, screening effective immunopotentiator is benefit for aquaculture, which may reduce the use of antibiotics. (peroxidasessignaling.com)

The need to control all these variables often leads to time-consuming and very expensive screening studies that require the analysis of a single variable at a time. (frontiersin.org)
AHCC mechanism of action was evaluated with immunoblotting and flow cytometry studies. (researchgate.net)

The major concern of chemotherapy is the side effects of existing drugs ( Baldo and Pham, 2013 ). (japsonline.com)
These nanocapsules are made of a PLGA-polymer matrix coated with Fe/FeO core-shell nanocrystals and co-loaded with chemotherapy drug and photothermal agent. (nature.com)

Hepatotoxicity (toxic effects on the liver) and cardiotoxicity (toxic effects on the heart) are the most severe problems encountered in drug development and the major reason for drug withdrawals. (holocausto.online)

Results: A quantitative assay of asparaginase was carried out and the level of production was found to be 1.8916 (Î¼mol/ml). (greenpharmacy.info)

Therefore, Src has been considered a target molecule for drug development. (oncotarget.com)

Moreover, according to the requirements of US Food and Drug Administration (FDA), theranostic agents should be completely cleared from the body within a reasonable period of time. (nature.com)

The plants had a fair amount of flavonoids and variable levels of cytotoxicity. (who.int)

Anatomy25

Organisms14

Diseases33

Chemicals and Drugs117

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes36

Disciplines and Occupations1

Technology, Industry, Agriculture5

Information Science1

Brine Shrimp Lethality Assay4

Vitro assays3

Phytochemical5

Potent3

Compounds7

Antibacterial1

Anticancer4

Antimicrobial1

Efficacy3

Induce3

Growth inhibition4

Bark3

Toxicity8

Tumor3

Activity8

Chemotherapeutic1

A5492

Viability2

Biologics1

Synthesis1

Anti-inflammatory2

Membrane2

Proliferation1

Antibodies2

DPPH1

Preclinical1

Compound2

Tumors1

Functional3

Immune2

Cells6

Cancer9

Biological activities1

Determination2

Potential1

Findings1

Virally1

Cell2

20191

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Results1

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Variable1