Erythroid k562 cells. Medical search. Frequent questions

The Sox6 transcription factor is crucial for terminal maturation of definitive red blood cells. (edu.sa)
Sox6-overexpression in K562 cells and in human primary ex vivo erythroid cultures enhances erythroid differentiation and leads to hemoglobinization, the hallmark of erythroid maturation. (edu.sa)
Sox6-overexpression induces dysregulation of 64 proteins, involved in cytoskeleton remodeling and in protein synthesis, folding and trafficking, key processes for erythroid maturation. (edu.sa)
AlaAcBu promoted cellular erythroid differentiation depicted by the expression of the marker glycophorin A and cellular maturation characterized by cytoplasm hemoglobinization, polar arrangement of mitochondria and a developed central vacuolar system preceding nuclear extrusion. (biu.ac.il)
Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. (molcells.org)
Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. (molcells.org)
During erythroid differentiation and maturation, three critical components α-globin, β-globin, and heme are critical for the formation of stable α2β2 hemoglobin (Hb) complexes. (longdom.org)
Heme synthesis enhances globin formation, not only because it is the precursor of Hb but also by modulating the machinery of gene expression and globin synthesis and coordinating erythroid cell maturation. (longdom.org)
ALA induced erythroid differentiation of human myelogenous leukemia K562 and murine erythroleukemia MEL cells, leads to hemoglobinization and cellular typical maturation. (longdom.org)
To form mature RBCs, the terminal maturation of erythroid cells involves two steps: (1) the condensation and expulsion of the nucleus to form reticulocytes and (2) the organelle clearance and remodeling of the membrane and proteome [ 1 , 7 ]. (biomedcentral.com)
However they do not exclude a role for in some other phase of erythroid progenitor maturation. (bioerc-iend.org)

Abaza L, Talorete TP, Yamada P, Kurita Y, Zarrouk M, Isoda H (2007) Induction of growth inhibition and differentiation of human leukemia HL-60 cells by a Tunisian gerboui olive leaf extract. (springer.com)

RAS GTPases are proto-oncoproteins that regulate cell growth, proliferation, and differentiation in response to extracellular signals. (bvsalud.org)
miR-29b suppressed cellular proliferation and promoted apoptosis of pulmonary artery smooth muscle cells, possibly through the inhibition of Mcl-1 and CCND2. (cusabio.cn)
data indicate that linc00598 plays an important role in cell cycle regulation and proliferation through its ability to regulate the transcription of CCND2. (cusabio.cn)
The surface immune molecule CD274 plays a critical role in the proliferation of leukemia-initiating cells, LICs. (cusabio.cn)
MiR-497 significantly suppressed cell proliferation by arresting the cell cycle through the CCND2 protein. (cusabio.cn)
or expression was down-regulated as previously described.9 Cell proliferation was analyzed by 3-(4 5. (bioerc-iend.org)
To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
The expression of RUNX1 mutant in ASXL1 -mutated myeloid cells augmented proliferation, blocked differentiation, and increased self-renewal activity. (biomedcentral.com)
The regulated expression and degradation of proteins are critical to all aspects of cell development and proliferation. (biomedcentral.com)
In fact, many studies have demonstrated the oleuropein and its derivative have proved pharmacological activity against proliferation of cancer cells and several tumor cell lines by different mechanisms such arrest cell cycle and cause apoptosis in cancerous cells, by modulation of miRNA expression and upregulation and downregulation of several genes. (springer.com)

To produce RBCs, HSCs differentiate into megakaryocyte-erythroid progenitors and then burst forming unit-erythroid (BFU-E) and colony-forming unit-erythroid (CFU-E). Upon erythropoietin (EPO) stimulation, CFU-E further develops into various erythroid precursors (proerythroblasts, basophilic erythroblasts, polychromatic erythroblasts, and orthochromatic erythroblasts). (biomedcentral.com)
Moreover, in human studies, JAK2V617F has been identified in the stem cell-enriched CD34 + CD38 − CD90 + Lin − compartment, common myeloid progenitors, granulocyte-monocyte precursors, and megakaryocyte-erythroid precursors as well as natural killer cells, B cells, and T cells in some patients, confirming its origin in an early hematopoietic progenitor. (oncohemakey.com)

Mice carrying a allele deleted in the third exon common to both and isoform display normal erythropoiesis.9 Together these findings suggest that is required for the differentiation of fetal erythroid progenitors. (bioerc-iend.org)
Our data indicate that is required for the expansion of commit ted erythroid progenitors whereas is crucial for terminal differentiation. (bioerc-iend.org)

Furthermore, by primer extension of single-stranded oligonucleotide probes, we show that the theta 1-globin gene of humans is transcribed in an erythroleukemia cell line K562. (ox.ac.uk)
Interestingly, in contrast to the alpha-globin genes, expression of the 3-methyladenine DNA glycosylase gene is not influenced by the regulatory element in the human erythroleukemia cell line K562. (ox.ac.uk)

The anti-SARS-CoV-2 BNT162b2 vaccine suppresses mithramycin-induced erythroid differentiation and expression of embryo-foetal globin genes in human erythroleukemia K562 cells, bioRvix. (davidicke.com)
RNA interference is a gene therapy that could potentially be used to "treat a variety of diseases" by delivering short strands of RNA that block specific genes from being turned on in a cell. (davidicke.com)
Using this gene therapy technique with specialised nanoparticles, researchers from Penn Engineering and the Massachusetts Institute of Technology ("MIT") developed a way to turn off specific genes in cells of bone marrow. (davidicke.com)
For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. (qedbio.com)
Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781). (qedbio.com)
Identifying causal variants and genes from human genetic studies of hematopoietic traits is important to enumerate basic regulatory mechanisms underlying these traits, and could ultimately augment translational efforts to generate platelets and/or red blood cells in vitro. (biomedcentral.com)
To identify putative causal genes from these data, we performed computational modeling using available genome-wide association datasets for platelet and red blood cell traits. (biomedcentral.com)
The major positive erythroid-specific regulatory element controlling alpha-globin gene expression lies equidistant between the promoters of the alpha-globin genes and the 3-methyladenine DNA glycosylase gene. (ox.ac.uk)
Co-expression of two mutant genes increased myeloid stem cells in animal model, suggesting that cooperation of RUNX1 and ASXL1 mutations played a critical role in leukemia transformation. (biomedcentral.com)
These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)

Recently we demonstrated that downregulation or over expression of ALA dehydratase (ALAD) and porphobilinogen deaminase (PBGD), the second and the third enzymes of the pathway, by specific shRNAs caused a marked alteration in PpIX synthesis in K562 erythroleukemic cells. (longdom.org)

We analyzed differential gene expression in the peripheral white blood cells (WBC) from 7 female patients diagnosed with occupational benzene poisoning and 7 matched controls. (cdc.gov)
Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)
Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
Hemoglobin synthesis and assembly is largely dependent on coordinated gene expression control, it is well established that the histone deacetylase inhibitor butyric acid activates transcription of heme synthesis an globin mRNA expression, leading to changes in cell morphology and induction of erythroid differentiation of erythroleukemic cell lines. (longdom.org)
Differentiation of multipotential hematopoietic cells into lineage-committed precursors involves the selection and maintenance of appropriate programs of gene expression, regulated by specific transcription factors. (abo.fi)
Using human K562 erythroleukemia cells capable of differentiating along erythroid and megakaryocytic lineages, we explore the differentiation-related role of heat shock transcription factor 2 (HSF2), which belongs to a family of transcription factors generally known to regulate heat shock gene expression. (abo.fi)
Our results reveal a multistep regulatory process of HSF2 gene expression, In K562 cells undergoing hemin-mediated erythroid differentiation, the increase in HSF2 protein levels is preceded by transcriptional induction of the HSF2 gene, accompanied by increased HSF2 mRNA stability. (abo.fi)
Pfizer and Moderna mRNA Vaccines Attack Bone Marrow Stem Cells and Drastically Alter Gene Expression I reviewed three recent papers: Sep. (itsonnews.com)
These results suggested that the cAMP-dependent pathway, which is independent of MAPK pathways, plays a negative role in γ-globin gene expression in K562 cells. (elsevierpure.com)
cAMP and cGMP may have differential roles in the regulation of γ-globin gene expression in erythroid cells. (elsevierpure.com)
Moreover, the RUNX1 mutant protein was more stable than WT and increased HIF1-α and its target ID1 gene expression in ASXL1 mutant cells. (biomedcentral.com)

Accordingly, the level of AQP0 high in early erythroid precursor cells may serve as a reference parameter for diagnosing diseases associated with dyserythropoiesis. (biomedcentral.com)
Our results provide strong evidence that the theta 1-globin gene of humans is transcriptionally active in cells of erythroid origin, and suggests the presence of a functional theta 1-polypeptide in specific cells, possibly those of early erythroid tissue. (ox.ac.uk)

After cell sorting, the gene expressions of membrane proteins in early and late erythropoiesis precursors and in nonerythroid cells were characterized using microarray analysis. (biomedcentral.com)
Among characterized gene candidates, aquaporin 0 (AQP0) expressed as a surface protein in early- and late-stage erythropoiesis precursors and was not expressed on nonerythroid cells. (biomedcentral.com)

Mechanistic analysis revealed that phenol, a major metabolite of benzene , significantly induced the expression of CYP4F3A at both mRNA and protein levels in cultured promyelocytic leukemia cells (HL-60) similarly to all-trans retinoic acid (ATRA). (cdc.gov)
This protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. (qedbio.com)
Meanwhile, read-through product of green fluorescence protein was also analyzed by Fluorescence Activated Cell Sorter. (whocc.org.cn)
Truncation of the binding site for the fetal/erythroid-specific stage selector protein in the γ-promoter abolished the additional enhancer activity of HS2. (monash.edu)
Similarly, insertion of the stage selector protein site into the β-promoter boosted enhancer activity observed with HS2 but not HS2(M). In adult erythroid MEL cells, enhancement of a linked β-promoter was significantly greater with HS2 than with HS2(M). This effect was dependent on the binding of the adult stage-specific factor, erythroid Kruppel-like factor, to the β-promoter. (monash.edu)
Here, we demonstrate that the S phase checkpoint protein CHK1 is maintained in a low phosphorylation state in Spi1/PU.1-overexpressing cells and provide evidence that this is not due to negative control of its primary kinase ATR. (oncotarget.com)
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. (cusabio.cn)
Our results provide novel insights into the function of EBNA3C on cell progression by regulating the cyclin D2 protein and raise the possibility of the development of new anticancer therapies against EBV-associated cancers. (cusabio.cn)
Quantification of Linoleylethanolamide dsRed+ cells from zebrafish embryos Transgenic embryos expressing green fluorescent protein (GFP) under the control of the promoter and red fluorescent protein (dsRed) under the promoter 16 were dissociated into single cells and analyzed by flow cytometry.14 O-dianisidine staining and heme content For O-dianisidine staining ten embryos/condition were collected and stained according to standard procedure. (bioerc-iend.org)

MiR-200b-3p depletion rescued the promoting effect of SP1 on SONFH cell model by activating Wnt/β-catenin pathway. (biomedcentral.com)
Taken together, our data suggest that HSF2 could function as a lineage-restricted transcription factor during differentiation of K562 cells along either the erythroid or the megakaryocytic pathway. (abo.fi)
The CD274/JNK/Cyclin D2 pathway promotes the cell cycle entry of LIC. (cusabio.cn)
The cAMP-dependent pathway was activated in K562 cells using the adenylate cyclase activator forskolin. (elsevierpure.com)
The cAMP-dependent pathway was found to be activated in K562 cells in which the cGMP-dependent pathway was activated by hemin. (elsevierpure.com)
Asgharzade S, Sheikhshabani SH, Ghasempour E, Heidari R, Rahmati S, Mohammadi M, Jazaeri A, Amini-Farsani Z (2020) The effect of oleuropein on apoptotic pathway regulators in breast cancer cells. (springer.com)

Induction of CYP4F3 by benzene metabolites in human white blood cells in vivo, in human promyelocytic leukemic cell lines, and ex vivo in human blood neutrophils. (cdc.gov)
Induction of CYP4F3 by phenol was also observed in differentiated HL-60 cells, in pro-erythroid cell line K562, and ex vivo in human neutrophiles. (cdc.gov)
This cell line proved to be insensitive to the induction of erythroid differentiation by PMEA. (aspetjournals.org)

The ability of AlaAcBu to promote differentiation along the erythroid lineage and to dramatically induce hemoglobin synthesis presented in this report. (biu.ac.il)
The findings demonstrated, for the first time, that benzene and metabolites induce CYP4F3 in human blood cells both in vivo and in vitro. (cdc.gov)

The acyclic nucleoside phosphonate derivative 9-(2-phosphonylmethoxyethyl) adenine (PMEA) is a strong inducer of differentiation in several tumor cell lines, including human erythroleukemia K562 cells. (aspetjournals.org)

We have compared the enhancer activity of this core element with a synthetic enhancer lacking the factor binding sites flanking the AP1/NF-E2 motif (HS2(M)). In fetal/erythroid K562 cells, enhancement of a linked γ-promoter was significantly greater with wild-type HS2 than with HS2(M). In contrast, the increase in β-promoter activity in these cells was equivalent with either enhancer fragment. (monash.edu)
17 Heme content was measured as described elsewhere.18 Erythropoiesis To obtain burst-forming units-erythroid (BFU-E) and colony-forming units-erythroid (CFU-E) 3 cells Linoleylethanolamide from fetal liver or adult bone marrow were cultured in MethoCult M3334 (Stemcell Technologies Vancouver Canada). (bioerc-iend.org)

In vivo hematopoietic stem cell modification by mRNA delivery. (davidicke.com)
STAT5 activation seems particularly important for JAK2V617F -induced cytokine independence in vitro and erythrocytosis in vivo and is in itself sufficient to support formation of endogenous erythroid colonies, a cardinal feature of PV. (oncohemakey.com)

To validate this putative candidate gene and to explore its function, we used CRISPR/Cas9 genome editing to discover that cultured TPM1 -deficient induced pluripotent stem cells enhanced hematopoietic progenitor cell formation. (biomedcentral.com)
Greater than 95% knockdown of the non-catalytic subunit CSN2 and the deneddylating subunit CSN5 of the CSN was achieved in the human myeloid progenitor cell line K562. (biomedcentral.com)

Our results show that the AlaAcBu mutual prodrug is a potent chemical differentiation inducer of K562 human erythroleukemia cells manifested by augmentation of heme and globin synthesis and assembly of hemoglobin. (biu.ac.il)
Exposure of K-562 cells to AlaAcBu resulted in an increase in heme synthesis and globin expression. (biu.ac.il)
With minimal instrumentation (four 96-well plates and a multichannel pipette), cell-specific codes are added to each tagged molecule within cells through sequential rounds of classical split-pool synthesis. (nature.com)

Accordingly, a goal of microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. (justia.com)
Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. (molcells.org)
Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation. (molcells.org)
In the present study, we identified miR-199b-5p as a positive erythroid regulator, that was regulated by the key erythroid transcription factors GATA-1 and NF-E2. (molcells.org)
The up-regulation of miR-199b-5p during erythroid differentiation was dependent on the binding of GATA-1 and NF-E2 to its gene locus, which activated its transcription and maintained its high expression level in mature erythroid cells. (molcells.org)
We previously showed that constitutive overexpression of the oncogenic transcription factor Spi1/PU.1 leads to pre-leukemic cells that have a shortened S phase duration with an increased replication fork speed and increased mutability in the absence of DNA breaks. (oncotarget.com)

On the other hand, hydroquinone induced extensive apoptosis of the cells. (cdc.gov)
Flow cytometry assessed cell apoptosis. (biomedcentral.com)
APS induced autophagy and suppressed apoptosis in SONFH cell model. (biomedcentral.com)
Recently, APS was reported to improve SONFH via regulating cell autophagy and apoptosis [ 9 ]. (biomedcentral.com)
24h after TCR transduction, CD107a expression correlated with low expansion rates due to apoptosis of ADRB3 specific T cells in contrast to CHM1 specific transgenic T cells. (oncotarget.com)

Regulation of c-jun mRNA expression by hydroxyurea in human K562 cells during erythroid differentiation. (meharryresearch.org)

If we could develop technologies that could control cellular activity in bone marrow and the hematopoietic stem cell niche, it could be transformative for disease applications," said Michael Mitchell, one of the study's lead authors. (davidicke.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Single-cell omics provide insight into cellular heterogeneity and function. (nature.com)
Treatment of vector control K562 cells with the autophagy inhibitors 3-methyladenine and bafilomycin A1 recapitulated the growth kinetics, vacuolar morphology and LC3-II accumulation of CSN2 knockdown cells indicating that the cellular phenotype of CSN2 cells arises from autophagy inhibition. (biomedcentral.com)
Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)

The activating effects of the mutation are thought to reflect disruption of the normal autoinhibitory function of the JH2 domain, within which JAK2V617F is found, and have been shown to affect downstream pathways, including STATs (especially STAT5), phosphatidylinositol 3-kinase, and MAP kinase pathways in cell lines and animal models. (oncohemakey.com)

Though red and white blood cells may end up in different places in the body, after we are born production of blood cells starts in the bone marrow. (davidicke.com)
Bone marrow makes more than 220 billion new blood cells every day. (davidicke.com)
Instead, they multiply rapidly in the bone marrow and interfere with blood cell production. (davidicke.com)
In a 2020 Nature Biomedical Engineering study, researchers used a type of genetic therapy, known as RNA interference, and nanoparticles modified in such a way that they would accumulate in the cells found in the bone marrow, rather than in the liver. (davidicke.com)
Mitchell was already working on new nanotechnologies that target bone marrow and immune cells for treating other diseases, especially blood cancers such as multiple myeloma. (davidicke.com)
In a paper published last week, Italian researchers treated lymphoblast cells isolated from the bone marrow of a 53-year-old chronic myelogenous leukaemia patient with Pfizer's mRNA covid injection, at increasing concentrations. (davidicke.com)
Additionally, decreased activity and abnormal differentiation of bone marrow mesenchymal stem cells may be an important mechanism in the occurrence and development of SONFH [ 5 ]. (biomedcentral.com)
Hematopoiesis occurs in the bone marrow, producing a complete spectrum of blood cells to maintain homeostasis. (biomedcentral.com)
Mice transduced with both ASXL1 and RUNX1 mutations enhanced inhibitor of DNA binding 1 (ID1) expression in the spleen, liver, and bone marrow cells. (biomedcentral.com)
In PV, bone marrow panmyelosis is associated with increased numbers of red blood cells and, in some patients, neutrophilia and/or thrombocytosis. (oncohemakey.com)

The chronic myeloproliferative neoplasms (MPNs) are clonal disorders characterized by overproduction of mature myeloid cells. (oncohemakey.com)
The MPNs comprise a set of clonal HSC disorders, characterized by the overproduction of 1 or more mature myeloid cell types. (oncohemakey.com)
We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)

To obtain an overview on processes downstream to Sox6 expression, we performed a differential proteomic analysis on human erythroid K562 cells overexpressing Sox6. (edu.sa)
Based on the differential expression levels of cell-surface molecules during erythropoiesis, flow cytometry analysis is a useful tool for analyzing the erythropoiesis process [ 8 ]. (biomedcentral.com)
Both CCND1- and CCND2-expressing MM cells arrested in S/G2/M, and did not differ in other cell-cycle proteins or sensitivity to IR.Differential expression of D-cyclin does not appear to affect cell-cycle response to IR, and is unlikely to underlie differential sensitivity to DNA damage. (cusabio.cn)
Microdroplets can barcode more cells but worries persist about the differential chemistry and resulting biases accomplished in each droplet. (nature.com)

Autologous as well as allogeneic CD8 + T cells transduced with tumor antigen specific T cell receptors (TCR) may cause significant tumor lysis upon adoptive transfer. (oncotarget.com)

However, because sufficient specific cell markers are scarce, dyserythropoietic diseases are challenging to identify through flow cytometry. (biomedcentral.com)
For erythroid differentiation single-cell suspensions were immunostained with anti-TER119-PE and anti-CD71-FITC (BD Italia Milan Italy) and analyzed by flow cytometry. (bioerc-iend.org)
The goals for advances in flow cytometry are clear: measure as many relevant target molecules per cell as quickly as possible. (nature.com)
Fluorescence and isotope tagging are the principal means for measuring antibody binding to cells in flow cytometry. (nature.com)

Haemin treatment of the K562 cells slightly enhances the level of the longest theta 1-transcript. (ox.ac.uk)

Lymphoblast cells are immature white blood cells that develop into healthy immune cells called lymphocytes. (davidicke.com)
Cell culture and gene silencing The human lymphoblast K562 cell line (ATTC number CCL-243? (bioerc-iend.org)

SONFH cell model was constructed using murine long bone osteocyte Y4 (MLO-Y4) cells and then treated with APS. (biomedcentral.com)

Zebrafish embryos were maintained according to standard procedures.14 Embryos were staged using hours post-fertilization (hpf) and morphological criteria.15 Zygotes were collected at one-cell stage and injected with 4 ng of oligomorpholino in the presence of phenol red for subsequent selection. (bioerc-iend.org)

In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. (molcells.org)
APS inhibited SP1 expression and SP1 overexpression reversed effects of APS on SONFH cell model. (biomedcentral.com)
We demonstrate that enhanced HSF2 expression and the acquisition of HSF2 DNA binding activity are strictly specific for erythroid characteristics of K562 cells. (abo.fi)
Notably, we found that the expression of the CHK1 phosphatase PP1α is increased in Spi1/PU.1-overexpressing cells. (oncotarget.com)
Sleeping Elegance is far more susceptible to more than expression inhibition than piggyBac and Tol2, the cargo capacity of Sleeping Attractiveness is constrained, and contrary to Tol2 and piggyBac that Inhibitors,Modulators,Libraries are lively in all mamma lian cell kinds tested, Sleeping Elegance display cell form dependent exercise. (p450inhibitors.com)

We conclude that CSN2 is required for CSN integrity and the stability of individual CSN subunits, and postulate that CSN2 loss results in a phenotype distinct from that of cells lacking CSN5 possibly as a consequence of altered CSN5 activity within a resultant CSN subcomplex. (biomedcentral.com)

The hnRNP K/E1-DICE complex interferes with the joining of the 60S ribosomal subunit to the 40S subunit at the AUG. We took advantage of the inducible human erythroid K562 cell system that fully recapitulates this process to identify so far unknown factors which are critical for DICE-dependent translational regulation. (researchatlanta.org)
Our data suggest that in premature erythroid cells translational silencing of hr15-LOX mRNA is usually maintained by DDX6 mediated storage in these RNP granules. (researchatlanta.org)

These particles could be tailored to help treat heart disease or to boost the yield of stem cells in patients who need stem cell transplants, Penn Engineering Today wrote. (davidicke.com)
As the dosage of Pfizer's injection increased, the growth of stem cells was inhibited. (davidicke.com)
CRISPR/Cas9-mediated TPM1 knockout in human induced pluripotent stem cells (iPSCs) enhanced hematopoietic progenitor development, increasing total megakaryocyte and erythroid cell yields. (biomedcentral.com)
All types of blood cells are derived from hematopoietic stem cells (HSCs). (biomedcentral.com)

LC-06-JCK-bearing mice showed high red blood cell counts and erythropoietin levels as compared to NTB mice, whereas MR16-1 treatment did not affect their levels. (biomedcentral.com)

A PMEA-resistant K562 cell line (designated K562/PMEA-1) was selected in the presence of escalating PMEA concentrations. (aspetjournals.org)
Nude mice were subcutaneously inoculated with cells of the IL-6-producing human lung cancer cell line LC-06-JCK and assessed as a model of cancer-related anemia. (biomedcentral.com)
Although the gene is widely expressed, the abundance of its mRNA is considerably higher in a colon adenocarcinoma cell line (HT29) than in other cell lines that were tested. (ox.ac.uk)

Although these systems provide a high-throughput qPCR readout, they do not address the front end sample preparation and require single cell isolation by FACS or micropipette followed by off-chip processing and pre-amplification of starting template prior to analysis. (justia.com)

However, unlike the PMEA derivatives, the antimetabolites remained equally cytostatic for the mutant K562 cells and for the wild-type cells. (aspetjournals.org)

In addition, because patients with dyserythropoiesis generally exhibited a reduced population of APQ0 high cells relative to healthy participants, the analysis results also suggested that the levels of APQ0 high cells in early erythropoiesis serve as a novel biomarker that distinguishes normal from dysregulated erythropoiesis. (biomedcentral.com)
Our results indicate that miR-4317 can reduce Non-small cell lung cancer (NSCLC) cell growth and metastasis by targeting FGF9 and CCND2. (cusabio.cn)
Results were compared to CHM1 319 -TCR transgenic T cells. (oncotarget.com)

AQP0 was successfully demonstrated to be a marker of erythroid differentiation. (biomedcentral.com)
We hypothesized lysosome-associated membrane glycoprotein 1 (LAMP1, CD107a) to be a marker for fratricide in TCR transgenic CD8 + T cells. (oncotarget.com)
CSN2 knockdown resulted in non-apoptotic cell death associated with accumulation of both the autophagy marker LC3-II and autophagic vacuoles. (biomedcentral.com)

Mutations in TET2, other epigenetic regulators, and other regulators of cytokine signaling are not specific to the classic myeloproliferative neoplasms (MPNs) but may influence prognosis and play roles in hematopoietic stem cell (HSC) dysregulation and progression to accelerated or blast-phase disease. (oncohemakey.com)

The highest virus yields, based on d-antigen concentrations, were observed using the recirculation mode for cell culture. (tyrosinekinases.com)

Microfluidic devices employing active valving to position and isolate cells have allowed for the isolation and genome amplification of individual microbial cells (32). (justia.com)

cyclin D2 acts as regulator of cell cycle proteins affecting SAMHD1-mediated HIV-1 restriction in non-proliferating macrophages. (cusabio.cn)
We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. (nature.com)

By exogenously modulating its activity, we demonstrate that PP1α is required to maintain CHK1 in a dephosphorylated state and, more importantly, that it is responsible for the accelerated replication fork progression in Spi1/PU.1-overexpressing cells. (oncotarget.com)
While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)

Anatomy29

Organisms9

Diseases20

Chemicals and Drugs72

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes38

Information Science3

Maturation11

Differentiation of human1

Proliferation10

Megakaryocyte-erythroid2

Progenitors2

Erythroleukemia cell line2

Genes11

Erythroleukemic1

Gene expression11

Early erythroid2

Precursors2

Protein9

Pathway6

Induction3

Induce2

Several tumor cell lines1

Fetal2

Vivo2

Progenitor cell2

Synthesis3

Transcription6

Apoptosis5

MRNA expression1

Cellular5

Pathways1

Bone marrow10

Myeloid3

Differential4

Tumor1

Flow cytometry4

Enhances1

Lymphoblast2

Murine1

Zebrafish1

Expression5

Subunits1

Translational2

Stem cells4

Erythropoietin1

Line3

FACS1

Mutant1

Results3

Marker3

Dysregulation1

Yields1

Genome1

Proteins2

Progression2