• proteins
  • If the topology of a cell membrane has yet to be determined, epitope insertion into proteins can be used in conjunction with immunofluorescence to determine structures. (wikipedia.org)
  • VlsE
  • Epitope mapping was carried out using microarrays that contained synthesized overlapping peptides covering the full sequence of VlsE from B. burgdorferi B31. (jpt.com)
  • In addition to the previously characterized IR6 region in the variable domain, specific sequences in the N- and C-terminal invariable domains of VlsE were found to be major B cell epitopes in affected patients. (jpt.com)
  • plasmas
  • The central hypothesis of this pilot project is to describe the spectrum of B-cell epitopes that are represented in hemophilia A inhibitor patient plasmas to provide data for statistical analysis to design a prospective clinical trial comparing epitope spectrum and response to treatment with fVIII and to results of immune tolerance therapy. (grantome.com)
  • analyze
  • We also address the methods used to analyze these epitopes, the relevance of their determination, and how this knowledge can inform studies on autoantibody pathogenicity. (biomedcentral.com)
  • assay
  • The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. (oup.com)
  • Relevance
  • Herein, we review reported target epitope(s) for known antibody-mediated disorders of the nervous system, address the relevance of these studies, explore the notion of epitope spreading, and discuss the issue of double autoantibody-positive patients. (biomedcentral.com)
  • region
  • Deleting the region bearing the LYP20 epitope, from a wild-type CD62P construct, showed a decrease in polymorphonuclear leucocyte (PMNL) binding to transfected COS cells. (biochemj.org)
  • sera
  • The patterns of epitope reputation did not Geldanamycin modification as time passes in females whose sera had been analyzed 9 years after their first-seropositive go to. (biodigestor.net)
  • further
  • By subjecting inserts from two clones into a second round of library construction and screening by immunoblot analysis, we further defined the epitope to EYLDMVLNETLRL. (aspetjournals.org)
  • good download epitope: country to Further freedoms in Analysis: carousel. (warren-nh.com)
  • response
  • We will compare the epitope spectrum to genotype as well response to treatment with fVIII in multiple in vitro coagulation assays. (grantome.com)
  • In a subset of patients we will have two samples from different points in time and we will compare the change of epitope spectrum and response to fVIII at the different time points. (grantome.com)
  • major
  • Antigen-presenting cells (APC) are capable of internalizing pathogens and displaying a fragment of the antigen - the epitope - with major histocompatibility complexes (MHCs) on the cell surface. (wikipedia.org)
  • patients
  • Inhibitor development is the most significant complication in the management of patients with hemophilia A. The goal of this project is to increase our knowledge of different B-cell epitope spectrum seen in hemophilia A patients who develop inhbitors. (grantome.com)
  • Furthermore, we discuss triggers of autoimmunity, such as molecular mimicry, ectopic antigen expression, epitope spreading, and potential mechanisms for the rising number of double autoantibody-positive patients. (biomedcentral.com)
  • However
  • however, other epitopes within the MPER were recognized by around one-third of HIV-1-infected individuals, although their neutralizing potential was not explored. (pubmedcentralcanada.ca)
  • found
  • One of the farms that probably decreased out to them from the terse download epitope mapping found the level that these beings spent in that calculus, and the zip that the weeks turned. (warren-nh.com)