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  • Microarray
  • The application note is supplemented by an Excel file with all microarray data of the assay, and can also be regarded as sample report for a typical PEPperMAP® Type 1 Epitope Mapping service. (pepperprint.com)
  • The Powerpoint file can also be regarded as typical sample report for a PEPperMAP® Epitope Substitution Scan and is supplemented by an Excel file with all microarray data . (pepperprint.com)
  • analysis
  • By subjecting inserts from two clones into a second round of library construction and screening by immunoblot analysis, we further defined the epitope to EYLDMVLNETLRL. (aspetjournals.org)
  • This technique provides the lowest cost, lowest sample consumption and shortest analysis time possible when compared with other conformational epitope mapping techniques. (covalx.com)
  • good download epitope: country to Further freedoms in Analysis: carousel. (warren-nh.com)
  • cell
  • PepSets peptide libraries make it possible to rapidly identify the epitopes of T-Cells, T-Cell clones, T-Helper Cell Clones, T-cell lines, and unpurified T-cells, such as lymph node cells and peripheral blood mononuclear cells (PBMC). (mimotopes.com)
  • New, proteomic approaches, including T-cell and B-cells-epitope mapping, can also accelerate the pace at which scientists discover antibody-antigen relationships. (wikipedia.org)
  • Antigen-presenting cells (APC) are capable of internalizing pathogens and displaying a fragment of the antigen - the epitope - with major histocompatibility complexes (MHCs) on the cell surface. (wikipedia.org)
  • identify
  • We demonstrate how IMMUNOCAT is utilized in a large-scale research contract that aims to identify epitopes in common allergens recognized by T cells from human donors, in order to facilitate the rational design of allergy vaccines. (hindawi.com)
  • procedure
  • To this end, we describe here an Array-in-Well-based screening procedure to perform activity testing and epitope mapping for filamentous phage-displayed scFvs in an integrated manner with a single assay. (jpt.com)
  • further
  • Obtaining information on the binding properties, such as the binding epitope, already during the screening step helps to choose the most useful candidates for further development at early phase saving time and resources. (jpt.com)
  • data
  • For this purpose, we have established "IMMUNOCAT", an interactive data management system for the epitope discovery research projects conducted by our group. (hindawi.com)
  • In addition to verifying antibody patentability, epitope mapping data has also been used to support broad antibody claims submitted to the United States Patent and Trademark Office. (wikipedia.org)
  • This technique can provide both epitope and paratope data in one experiment. (covalx.com)
  • work
  • Generation of an individual work plan for experimental setup and performance of epitope mapping experiments on a Biacore SPR instrument. (biaffin.com)
  • level
  • One of the farms that probably decreased out to them from the terse download epitope mapping found the level that these beings spent in that calculus, and the zip that the weeks turned. (warren-nh.com)
  • antibodies
  • We facilitate antibody generation for our clients by: consulting with our clients to find the best and most cost effective methods to meet their needs, generating of protein Immunogens using a number of expression systems, design custom protein immunogens to produced antibodies against linear as well as conformational epitopes. (ichorbiologics.com)
  • In tandem, the immunogen regions recognized by these antibodies are mapped allowing for the molecular modeling of antigen-antibody interactions. (ichorbiologics.com)
  • Unusually potent antibodies that neutralize human cytomegalovirus (HCMV) were isolated and their epitopes mapped to a pentameric glycoprotein complex, which is currently tested as a candidate vaccine. (wikipedia.org)
  • Thyroid peroxidase is a frequent epitope of autoantibodies in autoimmune thyroid disease, with such antibodies being called anti-thyroid peroxidase antibodies (anti-TPO antibodies). (wikipedia.org)
  • The immunoprecipitated complexes (i.e., the bead-antibody-protein-target DNA sequence complex) are then collected and washed to remove non-specifically bound chromatin, the protein-DNA cross-link is reversed and proteins are removed by digestion with proteinase K. An epitope-tagged version of the protein of interest, or in vivo biotinylation can be used instead of antibodies to the native protein of interest. (wikipedia.org)
  • It is important to note that most antibodies to modified histones are raised against unfixed, synthetic peptide antigens and that the epitopes they need to recognize in the XChIP may be disrupted or destroyed by formaldehyde cross-linking, particularly as the cross-links are likely to involve lysine e-amino groups in the N-terminals, disrupting the epitopes. (wikipedia.org)
  • Unlike some other tags (e.g. myc, HA), where a monoclonal antibody was first isolated against an existing protein, then the epitope was characterized and used as a tag, the FLAG epitope was an idealized, artificial design, to which monoclonal antibodies were raised. (wikipedia.org)
  • antigenic
  • Moreover, there are two antigenic epitopes (CypB84-92 and CypB91-99) recognized by HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes which could be used as cancer vaccines, and in fact, were used to treat lung cancer in a clinical trial. (wikipedia.org)
  • proteins
  • Cross-linked ChIP is mainly suited for mapping the DNA target of transcription factors or other chromatin-associated proteins, and uses reversibly cross-linked chromatin as starting material. (wikipedia.org)
  • The FLAG tag's structure was optimized for compatibility with proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. (wikipedia.org)
  • molecular
  • Some other applications that RPMA is used for include exploring and mapping protein signaling pathways, evaluating molecular drug targets and understanding a candidate drug's mechanism of action. (wikipedia.org)
  • likely
  • VAMP2 is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. (wikipedia.org)