• It is ubiquitously expressed in many tissues and cell types. (wikipedia.org)
  • Because of this and its ability to regulate multiple different ion channels, KCNE2 exerts considerable influence on a number of cell types and tissues. (wikipedia.org)
  • More recently, roles for KCNE proteins in a variety of non-cardiac tissues have also been explored. (wikipedia.org)
  • We demonstrate the general use of our approach by 4D imaging of organelles and fine structures in epithelial human cells, colonies of mouse embryonic stem cells, brain cells, and organotypic tissues. (nature.com)
  • Observing the interplay of organelles and macromolecular complexes inside living cells and tissues calls for the continuous development of minimally invasive optical systems performing at high spatio-temporal resolution. (nature.com)
  • A nanoscope able to record robust raw data rapidly and with sub-100 nm spatial resolution across the entire 3D space of cells and tissues is still missing. (nature.com)
  • Invasion and metastasis of malignant epithelial cells into normal tissues is accompanied by adaptive changes in the mesenchyme-derived supporting stroma of the target organs. (aacrjournals.org)
  • Dual specificity phosphatase 3 (DUSP3), a suppressor of multiple protein tyrosine (Tyr) kinases, is decreased in lung cancer tissues. (biomedcentral.com)
  • RNA sequencing (RNA-seq) analysis was performed on colon tissue to determine genes differentially expressed in intestinal tissues of mice. (healthanddietblog.info)
  • D) Traditional western evaluation of MAZ proteins appearance in cytosolic (lanes C) and nuclear (lanes N) ingredients from digestive tract tissues of WT and mice. (healthanddietblog.info)
  • They are expressed in single layer epithelial tissues of the body. (abnova.com)
  • The prepared antibody can be useful for the study of expression and distribution of IL-33 in various tissues at protein level and for the elucidation of its biofunctions and regulation mechanism in inflammatory diseases. (hindawi.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • chromatography on Ni-NTA Sepharose, and protein puri¢cation HFE mRNA is expressed in various tissues with the highest was con¢rmed by SDS-PAGE and Coomassie blue staining. (healthdrugpdf.com)
  • Nck-associated protein 1 (NAP1/NCKAP1) is highly expressed in primary non-small-cell lung cancer (NSCLC) when compared with adjacent normal lung tissues, and its expression levels are strongly associated with the histologic tumor grade, metastasis and poor survival rate of NSCLC patients. (biomedcentral.com)
  • CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. (thermofisher.com)
  • A single-cell layer of epithelium is separated from the tissues beneath it by a supporting substance called the extracellular matrix. (elifesciences.org)
  • Understanding how cancerous cells gain this ability may lead to new approaches to stopping tumor cells from dividing and colonizing tissues around the body. (elifesciences.org)
  • This behavior is particularly evident in the cells that divide aggressively to form tumors that subsequently migrate and colonize other tissues around the body. (elifesciences.org)
  • In addition, as the genetic identity of the donor egg from which the ESCs are derived most likely will differ from that of potential recipients, patients who receive ESC-derived cells or tissues may face the same complications that result from organ transplantation (for example, immunorejection, graft-versus-host disease, and need for immunosuppression). (biomedcentral.com)
  • pathomechanisms that regulate extravasation of damaging immune cells into surrounding tissues are poorly understood. (nature.com)
  • RFC is ubiquitously expressed in normal tissues and some tumors. (biomedcentral.com)
  • Resting macrophages, which are abundant in normal tissues and participate in homeostasis, have not been found to express FRβ. (biomedcentral.com)
  • Among these features are the peculiar tropism of the virus for Vero cells (a continuous cell line established from monkey kidney epithelial cells), its capacity for growth at 37°C (while other respiratory coronaviruses grow at lower temperatures), and its ability to infect lower respiratory tract tissues ( 13 ). (cdc.gov)
  • Tissues and Cells. (lu.se)
  • cDNA for MAZ expressing an N-terminal FLAG tag was cloned into the previously explained 12.4-kb promoter construct to restrict MAZ expression to the intestine epithelium, here referred to as mice (21). (healthanddietblog.info)
  • The IL-33 cDNA sequences encode 270 and 266 amino acid polypeptides for human and mouse, respectively, corresponding to full-length proteins with calculated masses of 30 and 29.9 kDa [ 2 ]. (hindawi.com)
  • The HFE Cys282CTyr Full length human HFE cDNA was generated by RT-PCR from mutation, which is homozygous in s 70% of HHC patients mRNA extracted from human pulmonary cells. (healthdrugpdf.com)
  • The cDNA was sub- cloned into pCDNA3.1 vector (Invitrogen) and pUDH10-3 vector [3^5], results in the loss of a structural disul¢de bond, prevents (Clontech) fused to the myc-tag peptide at the C-terminus. (healthdrugpdf.com)
  • The association with L2-microglobulin (L2m) and proper presenta- cDNA sequence encoding HFE ectodomains (residues 26^304) was tion to cell surfaces [6,7]. (healthdrugpdf.com)
  • The hOAT3 cDNA consisted of 2179 base pairs that encoded a 543-amino-acid residue protein with 12 putative transmembrane domains. (aspetjournals.org)
  • 1985). The c-cDNA was cloned and shown to encode a typical class III receptor tyrosine kinase (RTK) (Coussens GSI-IX et al. (techblessing.com)
  • Malignant epithelial cancers, the major cause of cancer morbidity and mortality, arise in organs composed of both epithelial and mesenchyme-derived stromal cells, such as fibroblasts, myofibroblasts, endothelial cells, pericytes, smooth muscle, and hematopoietic cells. (aacrjournals.org)
  • It is also expressed on endothelial cells, epithelial cells, bone marrow stroma cells, and osteoclasts, as well as a small proportion of LGL lymphocytes. (healthweblognews.info)
  • We studied the effects of an Acanthamoeba encephalitis isolate belonging to the T1 genotype on human brain microvascular endothelial cells, which constitute the blood-brain barrier. (asm.org)
  • Using an apoptosis-specific enzyme-linked immunosorbent assay, we showed that Acanthamoeba induces programmed cell death in brain microvascular endothelial cells. (asm.org)
  • These results were further confirmed using brain microvascular endothelial cells expressing dominant negative forms of phosphatidylinositol 3-kinase. (asm.org)
  • We have demonstrated that pathogenic Acanthamoeba exhibits more than 60% binding to human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier ( 2 ). (asm.org)
  • Enhanced ZNF281 transcriptionally regulated CCL2 and CCL5 expression to activate P38 MAPK signaling in endothelial cells. (thno.org)
  • Functional studies revealed increased expression of ICAM-1 on induced patient-derived endothelial cells (iECs) and of β2-integrins on patient neutrophils that increase neutrophil adhesion and vascular transendothelial migration (TEM). (nature.com)
  • We characterize the role of increased Lyn kinase activity in neutrophils, endothelial cells and lesional liver biopsies and utilized an iPSC-derived endothelial cell platform for disease modeling of neutrophilic vasculitis and to screen and evaluate drug efficacy. (nature.com)
  • After sensitization with cytotoxic drugs, scFv62-TRAIL induced apoptosis only in K V 10.1-positive cancer cells, but not in non-tumor cells, nor in tumor cells lacking K V 10.1 expression. (biomedcentral.com)
  • In co-cultures with K V 10.1-positive cancer cells the fusion protein also induced apoptosis in bystander K V 10.1-negative cancer cells, while normal prostate epithelial cells were not affected when present as bystander. (biomedcentral.com)
  • Analysis of chemically distinct conjugates, resistance models, and biomarkers implicates a unique mode of action, with mitotic arrest and apoptosis of malignant epithelial cells coupled to disruption of fibroblastic and vascular structures. (aacrjournals.org)
  • The absence of NuRD triggers apoptosis via the EGL-1-CED-9-CED-4-CED-3 pathway, while an ectopic gain of NuRD enables apoptotic daughter cells to survive. (elifesciences.org)
  • These results demonstrate a functional role for PMS2 to protect against PCa progression by enhancing apoptosis of PCa cells. (oncotarget.com)
  • A vinculin fragment (amino acids 811-1066) containing the paxillin binding site restored apoptosis and suppressed ERK activity in vin −/− cells. (rupress.org)
  • Transfection with paxillin Y31FY118F dominant-negative mutant in these cells inhibits ERK activation and restores apoptosis. (rupress.org)
  • CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions. (thermofisher.com)
  • Wnt ligands are secreted glycoproteins that function in intracellular signaling pathways to regulate a variety of developmental processes including cell growth, cell differentiation, cell polarity, and apoptosis. (biomedcentral.com)
  • In vitro studies showed that IL-7 induces oocyte maturation and inhibits apoptosis in granulosa cells. (biolegend.com)
  • 2007). We discover that ISWI is certainly a RanGTP-dependent microtubule-associated proteins (MAP) in vitro. (researchensemble.com)
  • DUSP3/VHR, related to the vaccinia virus phosphatase VH1, is initially found to be a phosphatase that dephosphorylates multiple protein tyrosine kinases (PTKs) in vitro [ 21 ]. (biomedcentral.com)
  • The precursor protein (pre-IL-33) was cleaved by caspase-1 into a mature 18-kDa protein (IL-33) in in vitro experiments using a recombinant protein [ 2 ]. (hindawi.com)
  • Examples of a novel series of phenanthrolinones are shown to be potent competitive inhibitors of avian prolyl 4-hydroxylase, and of collagen hydroxylation, in embryonic chick tendon cells and human foreskin fibroblasts in vitro and in the oestradiol-stimulated rat uterus in vivo. (embl.de)
  • Nck-associated protein 1 (NAP1/NCKAP1), a protein that associates with the Src homology 3 (SH3) domain of NCK protein both in vitro and in intact cells, was found to localize along the lamellipodia and to mediate contact-dependent cell migration [ 4 ]. (biomedcentral.com)
  • 2008. A plant virus movement protein forms ringlike complexes with the major nucleolar protein, fibrillarin, in vitro . (hutton.ac.uk)
  • IL-7 was initially described as a stromal derived factor which is capable of inducing the growth of pre-B cells in vitro . (biolegend.com)
  • In vitro, the recombinant tagged viruses exhibited morphologies, plaque, and growth kinetics similar to the parental BTV-16, and expressed both VP2 and HA tag. (morulaivf.com)
  • Antibody-dependent cell-mediated cytotoxicity (ADCC) was evaluated with FRβ positive CHO cells as target cells and isolated peripheral blood monocytes as effector cells in an in vitro assay. (biomedcentral.com)
  • To gain insight into SARS-CoV biopathology, we analyzed the relevant features of SARS-CoV HSR1 growth in vitro, including the ultrastructural analysis of the consequences of virus replication in Vero cells. (cdc.gov)
  • The cells stained positive with PAb416, a monoclonal antibody against the SV40 large T antigen. (cdc.gov)
  • After generation of hybridomas, we identified clones producing antibody against WU-VP1 by ELISA and immunoblot. (cdc.gov)
  • The K V 10.1-specific scFv62 antibody -TRAIL fusion protein was expressed in CHO-K1 cells, purified by chromatography and tested for biological activity. (biomedcentral.com)
  • We could design a strategy that selectively kills tumor cells based on a K V 10.1-specific antibody. (biomedcentral.com)
  • The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)
  • A novel antibody-maytansinoid conjugate, monoclonal antibody (mAb) FAP5-DM1, was developed to target a shared epitope of human, mouse, and cynomolgus monkey fibroblast activation protein-α, enabling preclinical efficacy and tolerability assessments. (aacrjournals.org)
  • Western Blot analysis of KRT18 expression in transfected 293T cell line by KRT18 monoclonal antibody (M01), clone 2F8. (abnova.com)
  • Immunoprecipitation of KRT18 transfected lysate using anti-KRT18 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with KRT18 MaxPab rabbit polyclonal antibody. (abnova.com)
  • Detection limit for recombinant GST tagged KRT18 is approximately 0.1ng/ml as a capture antibody. (abnova.com)
  • Immunofluorescence of monoclonal antibody to KRT18 on HeLa cell. (abnova.com)
  • Antibody Reactive Against Recombinant Protein. (abnova.com)
  • In this study, the cloning and expression of mouse IL-33 gene in Escherichia coli (E. coli ), purification of recombinant protein, and generation of polyclonal antibody against IL-33 are described. (hindawi.com)
  • This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • The generation of IL-7-deficient and IL-7Rα-deficient mice and monoclonal antibody blocking experiments confirmed the requirement of IL-7 for B-cell development in mice. (biolegend.com)
  • To further investigate the function of the US11 protein, this study was undertaken to express the US11 protein and raise a polyclonal antibody. (biomedcentral.com)
  • After purification by nickel affinity chromatography and refolding, the recombinant protein was used to raise the anti-US11 polyclonal antibody. (biomedcentral.com)
  • Western blot analysis demonstrated that the US11 protein was specifically recognized by the polyclonal antibody, and immunofluorescent assay also showed that the antibody was able to probe the US11 protein in the cells infected with HSV-1. (biomedcentral.com)
  • In the present study, we obtained a high-level expression of the recombinant US11 protein as well as high titers of rabbit polyclonal antibody specially against US11 protein in HSV-1 infected cells. (biomedcentral.com)
  • This special polyclonal antibody provides a good tool for further studying structural and functional characterization of HSV-1 US11 protein. (biomedcentral.com)
  • Subsequently, a polyclonal antibody was raised against the purified His-tagged US11 protein in rabbits. (biomedcentral.com)
  • Subsequently, the selected recombinant tagged viruses were prepared as inactivated vaccines to immunize IFNAR(-/-) mice and sheep, and serological detection results of an anti-HA antibody provided discriminative detection. (morulaivf.com)
  • Two broadly neutralizing monoclonal antibodies derived from NIV-immunized mice were characterized by transmission electron microscopy (TEM), antibody competition assays, fluorescence-activated cell sorting (FACS) analysis, and protein-protein docking. (morulaivf.com)
  • Sequences of DNA in the genes that are located between the EXONS. (lookformedical.com)
  • RESULTS Intestinal epithelial MAZ regulates a novel repertoire of genes. (healthanddietblog.info)
  • Many book genes, including a genuine variety of inflammation-associated genes governed by epithelial MAZ appearance, were discovered (Fig. 1F). (healthanddietblog.info)
  • Oddly enough, just 24% of considerably changed genes discovered in RNA-seq evaluation corresponded with discovered genes in RNA-seq from digestive tract tissue of HIF-2-expressing pets (10), recommending a repertoire of HIF-2-indie focus on genes (Fig. 1G). (healthanddietblog.info)
  • These outcomes identify a book repertoire of MAZ-regulated genes in digestive tract epithelial cells, a lot of which usually do not overlap genes regulated with HIF-2 directly. (healthanddietblog.info)
  • Abundance and distribution of archaeal Group I (MCGI) genes in the coastal Arctic and the ratio archaeal 16S rDNA gene abundance in the Atlantic (D), and ratio high-ammonia focus 0.7) in the amount of OTUs attained by cloning with the HAC and the LAC primers seeing that indicated by the rarefaction curves shown in CA-074 Methyl Ester novel inhibtior Fig. S7. (healthweblognews.info)
  • the protein levels of related genes (e.g. (thno.org)
  • Genetic knockdown of the particular genes in cancer cells were achieved by lentiviral-mediated interference. (biomedcentral.com)
  • We then focused on two genes previously linked to various cancers, mesothelin and stromelysin-1, which are respectively up-regulated by Wnt-1 and Wnt-5a in C57mg cells. (biomedcentral.com)
  • A gain of function screen for genes that enable proliferation independently of matrix anchorage identified a cell adhesion molecule PVRL4 (poliovirus-receptor-like 4), also known as Nectin-4. (elifesciences.org)
  • explored which genes enable epithelial cells from the human mammary gland to grow without being attached to the extracellular matrix. (elifesciences.org)
  • 2009. Characterisation of mjap genes encoding novel secreted proteins from the root-knot nematode, Meloidogyne javanica . (hutton.ac.uk)
  • During productive infection, the 152-kb double-stranded HSV-1 genome is rapidly translocated to the nucleus where at least 80 viral genes are transcribed by the host cell RNA polymerase II (Pol II) [ 1 ]. (biomedcentral.com)
  • The US11 protein expresses at late times during HSV-1 infection and is one of the late genes of HSV-1 [ 3 ]. (biomedcentral.com)
  • KRT18 (AAH00180, 1 a.a. ~ 430 a.a) full-length recombinant protein with GST tag. (abnova.com)
  • The recombinant protein, IL-33 containing a hexahistidine tag in the C-terminal, was expressed in Escherichia coli . (hindawi.com)
  • The rabbits were immunized with the purified recombinant protein. (hindawi.com)
  • The high titer (1:32000) polyclonal antibodies with high specificity were obtained by immunizing rabbits with the purified recombinant protein. (hindawi.com)
  • The 149 amino acid recombinant protein has a predicted molecular mass of approximately 16.9 kD. (biolegend.com)
  • Members of this family appear to play a role in regulation of epithelial proliferation. (origene.com)
  • RON is activated in response to macrophage-stimulating protein (MSP), and then induces an invasive program ( 8 ) consisting of cell proliferation, migration, and invasion, all of which are important at multiple points during tumorigenesis. (spandidos-publications.com)
  • CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. (thermofisher.com)
  • Folate receptor beta (FRβ) is only detectable in placenta and limited to some hematopoietic cells of myeloid lineage in healthy people. (biomedcentral.com)
  • in turn, the malignant epithelial cells can undergo reversible epithelial-mesenchymal transitions ( 1 - 5 ). (aacrjournals.org)
  • During oncogenesis, cancer cells undergo epithelial-mesenchymal transition (EMT), lose their polarity, and gain higher migratory and invasive abilities. (biomedcentral.com)
  • During all stages of tumor progression, cancer cells are subjected to inappropriate extracellular matrix environments and must undergo adaptive changes in order to evade growth constraints associated with the loss of matrix attachment. (elifesciences.org)
  • The individual cells within a single-cell layer are physically attached to the matrix, and when displaced from it, they promptly undergo programmed cell death. (elifesciences.org)
  • In this process, astrocytes proliferate to fill gaps and undergo a typical morphological change: a large cytoplasmic mass, long and branched processes, and increased expression of intermediate filaments such as glial fibrillary acidic protein (GFAP). (jneurosci.org)
  • This innate immunity is accomplished by pattern-recognition receptors such as the toll-like receptors (TLRs) (Kaisho and Akira, 2006) or retinoic acid inducible gene I (RIG-I)-like receptors (RLRs) of the host cells (Yoneyama and Fujita, 2004), followed by production of antiviral cytokine interferons (IFNs). (datexis.com)
  • Vertebrate Wnts signal through frizzled receptors that in some experimental contexts (e.g., without co-expressing LRP5/6) preferentially activate the Wnt/beta-catenin or Wnt/Ca 2+ pathways [ 5 ]. (biomedcentral.com)
  • Cry1Ia1 is a protein that is toxic to potato tuber moth, and acts by binding to specific receptors on the membranes of epithelial cells in the target insect's midgut. (ashs.org)
  • In addition to RFC and PCFT, a limited number of cells express folate receptors (FRs) that can mediate unidirectional transportation of folates into cells. (biomedcentral.com)
  • However, cell-cell junctions do not always negatively regulate cancer development. (biomedcentral.com)
  • The evolutionarily conserved polarity proteins PAR-3, atypical protein kinase C (aPKC) and PAR-6 critically regulate the apical membrane development required for epithelial organ development. (silverchair.com)
  • Previously we found that mitotic factors regulate epithelial-mesenchymal transition, but how these factors convert to metastatic players in the tumor microenvironment (TME) is not fully understood. (biomedcentral.com)
  • CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. (thermofisher.com)
  • Beta-catenin then accumulates in the nucleus where it interacts with its transcriptional co-activators, members of the Lymphoid Enhancer Factor/T Cell Factor (LEF/TCF) family of high mobility group DNA binding proteins to regulate gene expression [ 1 ]. (biomedcentral.com)
  • Acanthamoeba -mediated brain endothelial cell death was abolished using LY294002, a phosphatidylinositol 3-kinase inhibitor. (asm.org)
  • This is the first demonstration that Acanthamoeba -mediated brain microvascular endothelial cell death is dependent on phosphatidylinositol 3-kinase. (asm.org)
  • Here, we show that vinculin null (vin −/− ) cells and cells expressing a vinculin Y822F mutant have increased survival due to up-regulated activity of extracellular signal-regulated kinase (ERK). (rupress.org)
  • Both vinY822F and vin −/− cells exhibit increased interaction between paxillin and focal adhesion kinase (FAK) and increased paxillin and FAK phosphorylation. (rupress.org)
  • Recepteur d'origine nantais (RON) is a receptor tyrosine kinase (RTK) normally expressed at low levels in epithelial cells. (spandidos-publications.com)
  • RON is a 180-kDa heterodimeric protein composed of a 40-kDa α-chain and a 150-kDa transmembrane β-chain with intrinsic tyrosine kinase activity. (spandidos-publications.com)
  • Maturation occurs in the cell membrane resulting in a 180-kDa heterodimeric protein composed of a 40-kDa α-chain and a 150-kDa transmembrane β-chain with intrinsic tyrosine kinase activity ( 5 , 6 ). (spandidos-publications.com)
  • Next-generation sequencing identified two de novo truncating variants in the Src-family tyrosine kinase, LYN , p.Y508*, p.Q507* and a de novo missense variant, p.Y508F, that result in constitutive activation of Lyn kinase. (nature.com)
  • Similar to other family members it possesses a highly glycosylated extracellular region comprised of five immunoglobulin domains (D1-D5 498 amino acids) a transmembrane domain name (21 amino acids) and an intracellular area made up of a juxtamembrane area GSI-IX (JMD) (36 proteins) and an intracellular tyrosine GSI-IX kinase area (398 proteins) that's interrupted with a kinase put area (73 proteins) (Fig. 1A) (Coussens et al. (techblessing.com)
  • We have used stroma-rich models in immunodeficient mice, which recapitulate the histotypic arrangement found in human epithelial cancers. (aacrjournals.org)
  • Our study revealed that DUSP3 is an important TJ regulatory protein and its decrease may be involved in progression of epithelial cancers. (biomedcentral.com)
  • therefore, abnormalities of TJ are frequently observed in epithelial cancers [ 4 ]. (biomedcentral.com)
  • It is estimated that up to 90% of cancers in humans originate in epithelial tissue, and the cells within such tumors are known to survive and divide even when they are no longer attached to the extracellular matrix. (elifesciences.org)
  • ER protein determinations were performed using two-thirds of all breast cancers are ER at the time of diagnosis, the standard methods in the routine clinical laboratory (9). (lu.se)
  • In normal cells, folates are taken in through the reduced folate carrier (RFC) or proton-coupled folate transporter (PCFT), which are membrane-spanning proteins that facilitate bidirectional transportation of reduced folate across the plasma membrane and endosome membranes [ 1 ]. (biomedcentral.com)
  • Cell-cell interaction has been shown to coordinate collective cell migration, which is a critical process in cancer metastasis [ 6 ]. (biomedcentral.com)
  • Understanding the mechanism behind immune cell plasticity in cancer metastasis is crucial for identifying key regulators. (biomedcentral.com)
  • Exploring the mechanism of the plasticity of immune cells triggered by cancer cells through crosstalk is important for discovering key regulators to manage cancer metastasis. (biomedcentral.com)
  • Genetic reprogramming is critical to acquire the mesenchymal characteristics during metastasis that affect the TME through crosstalk between cells. (biomedcentral.com)
  • Mechanistic studies further reveal that the binding of NAP1 to the cellular chaperone heat shock protein 90 (HSP90) is required for its protein stabilization, and NAP1 plays an essential role in HSP90-mediated invasion and metastasis by provoking MMP9 activation and the epithelial-to-mesenchymal transition in NSCLC cells. (biomedcentral.com)
  • Our insights demonstrate the importance and functional regulation of the HSP90-NAP1 protein complex in cancer metastatic signaling, which spur new avenues to target this interaction as a novel approach to block NSCLC metastasis. (biomedcentral.com)
  • WASF3 is a tumor metastasis driver in breast cancer, and its knockdown leads to a significant reduction in metastatic breast cancer cell invasion and metastasis in mice [ 5 ]. (biomedcentral.com)
  • Our previous studies further demonstrated that NAP1 is required for the protein stability of WASF3 in breast cancer cells, implicating that NAP1 is a critical regulator in favor of breast cancer metastasis [ 5 ]. (biomedcentral.com)
  • Estrogens are important regulators of growth and differentiation in (range, 0 -9 fmol/mg protein, median 0.7). (lu.se)
  • Tight junctions (TJ) are multi-protein complexes that hold epithelial cells together and form structural and functional barriers for maintaining proper biological activities. (biomedcentral.com)
  • DNA mismatch repair (MMR) enzymes act as proofreading complexes that maintains genomic integrity and MMR-deficient cells show an increased mutation rate. (oncotarget.com)
  • These indices of in the endoplasmic reticulum/cis-Golgi compartment soon cellular iron deficiency were reversed by iron supplementation after synthesis, it stabilizes HFE protein from degradation complexes. (healthdrugpdf.com)
  • Adhesion complexes (i.e., focal adhesions and focal complexes) and cell-cell contacts are specialized structures that harbor a large number of cytoskeletal proteins and one of the highest concentrations of signaling molecules in cells ( Ruoslahti and Obrink, 1996 ). (rupress.org)
  • Then, proteins encoded by the nuclear genome and destined for the mitochondria will be made close to mitochondrial-encoded proteins so that they can be assembled efficiently into mitochondrial complexes. (biomedcentral.com)
  • Cloning and expression of the mouse SLC6A19 gene demonstrated that this transporter has all the properties of the amino acid transport system B 0 AT1. (medscape.com)
  • This is especially relevant in dim structures with moderately low signal to noise ratio (SNR), such as in cells exhibiting endogenous levels of rsFP fusion expression and in 3D samples where out-of-focus background dominates. (nature.com)
  • Altered gene expression in these nontransformed stromal cells provides potential targets for therapy. (aacrjournals.org)
  • Compared to those of the DUSP3-expressing cells, we found the expression and distribution of ZO-1, a TJ-anchoring molecule, were abnormal in DUSP3-deficient cells. (biomedcentral.com)
  • MAZ and FLAG protein expression were verified from colon and small intestine lysates from wild-type (WT) and mice (Fig. 1A). (healthanddietblog.info)
  • Nevertheless, MAZ proteins expression hasn't been examined in inflammatory disease from the digestive tract. (healthanddietblog.info)
  • Oddly enough, increased MAZ proteins expression had not been seen in ileal TAK-960 hydrochloride biopsy specimens from Crohn's disease (Compact disc) (Fig. 2C and ?andD).D). This suggests a potential function for digestive tract GLCE epithelial MAZ in the inflammatory development of colitis. (healthanddietblog.info)
  • The sequences of the constructs were first gene-optimized for vector expression and synthesized, representing in total 186 expression clones. (europa.eu)
  • The coding region of IL-33 mature protein was cloned into the prokaryotic expression vector pET-44. (hindawi.com)
  • The regulation of expression of X-box-binding protein-1 (XBP1), a transcriptional factor, involves an unconventional mRNA splicing that removes the 26 nucleotides intron. (mdpi.com)
  • Previous studies have shown PMS2 to be deficient in DU145 cells and this lack of expression was confirmed by Western blotting whereas normal prostatic PWR-1E and RWPE-1 cells expressed this gene. (oncotarget.com)
  • liver and brain sinusoidal cells, and in scattered epithelial cells HFE expression was fully repressed by the presence of in the crypts of the small and large intestine [13]. (healthdrugpdf.com)
  • DNA, expression and puri¢cation of HFE protein ity complex class I molecules [2]. (healthdrugpdf.com)
  • Mice deleted for the HFE or the subcloned into vector pET12b for expression in Escherichia coli, fused L2m gene show a fast accumulation of iron in the parenchyma to a polyhistidine tag. (healthdrugpdf.com)
  • The arti- of E. coli strain BL21(DE3)pLysS and protein expression were per- ¢cially induced C282Y mutation in mice produces an HHC- formed essentially as described [19]. (healthdrugpdf.com)
  • Analysis of heterologous expression systems of rOAT1 in oocytes and culture cells revealed that rOAT1 has the ability to transport anionic drugs, such as β-lactam antibiotics, NSAIDs, methotrexate, and antiviral drugs, as well as various endogenous organic anions and exogenous substances. (aspetjournals.org)
  • Co-expression of Ov-phy-1 with the O. volvulus homologue of protein disulfide isomerase in a baculovirus system resulted in the production of enzymatically active O. volvulus prolyl 4-hydroxylase. (embl.de)
  • To determine the effects of osmolarity/tonicity changes, Wnt/β-catenin and TLR-4 activation on Lcn2-R and Lcn2 expression and cell viability in rat primary IMCD and mouse (m)IMCD3 cells. (biomedcentral.com)
  • Hyperosmotic/-tonic media upregulated Lcn2-R by ~4-fold and decreased Lcn2 expression/secretion, along with Wnt/β-catenin activation, in IMCD cells. (biomedcentral.com)
  • These survival mechanisms include accumulation of organic osmolytes and increased expression of heat shock proteins through numerous pathways, resulting in osmotolerance. (biomedcentral.com)
  • CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. (thermofisher.com)
  • CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. (thermofisher.com)
  • To test the hypothesis that a specific cell line can respond to distinct Wnts with different patterns of gene expression, we over-expressed Wnt-5a and Rfz-2 in C57mg mammary epithelial cells and compared this cell line to C57mg cells over-expressing Wnt-1. (biomedcentral.com)
  • Different Wnts have distinct effects on gene expression in a single cell line. (biomedcentral.com)
  • This gene has no protein-coding homolog in any other genome but its presence is supported by evidence from expression and hybridization data. (ubc.ca)
  • In response to a brain injury, astrocytes proliferate and become hypertrophic with an increased expression of intermediate filament proteins. (jneurosci.org)
  • Forced expression of lcn2 or treatment with LCN2 protein increased the sensitivity of astrocytes to cytotoxic stimuli. (jneurosci.org)
  • In zebrafishes, forced expression of rat lcn2 gene increased the number and thickness of cellular processes in GFAP-expressing radial glia cells, suggesting that lcn2 expression in glia cells plays an important role in vivo . (jneurosci.org)
  • In 2006, a major breakthrough was reported in Japan by Takahashi and Yamanaka, who described the generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts via over expression of defined transcription factors [ 2 ]. (biomedcentral.com)
  • CSF-1R Expression The CSF-1R is usually expressed at low levels on hematopoietic stem cells (HSCs) (Sarrazin et al. (techblessing.com)
  • The US11 gene was cloned into the prokaryotic expression vector pET-32a (+) to express His-tagged US11 protein in Escherichia coli. (biomedcentral.com)
  • We describe the functional analysis methods for HA, including the expression of recombinant proteins, binding to glycoproteins and epithelial cells, and localization in mouse intestinal tissue. (morulaivf.com)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
  • Mutations in IL-7Rα observed in patients with severe combined immunodeficiency (SCID) also underlies the importance of IL-7 signaling in T-cell development in humans. (biolegend.com)
  • proto-oncogene (oncogene encoded by the Susan McDonough strain of feline sarcoma computer virus (SM-FeSV) … Comparison of the sequences of cat c-and a v-retroviral oncogene derived from it revealed that a carboxy-terminal truncation together with two point mutations (L301S and A374S) in the extracellular D4 domain name subsequently shown to contain the dimerization interface (Elegheert et al. (techblessing.com)
  • There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. (lookformedical.com)
  • In most human cells, mitochondria contain 10 3 -10 4 copies of a circular genome of 16,569 base-pairs that encodes two ribosomal RNAs, 22 tRNAs, and 13 polypeptides that form parts of the respiratory-chain located in the inner mitochondrial membrane [ 9 , 10 ]. (biomedcentral.com)
  • The full-length US11 gene, which is composed of 459 bp (base pairs) and predicted to encode a protein of 152 amino acids, was amplified successfully from the HSV-1 (strain F) genome (Figure 1 , lane 1). (biomedcentral.com)
  • The full-length viral genome sequence was similar to those derived from the Hong-Kong Hotel M isolate. (cdc.gov)
  • Sequence analysis of the complete genome of SARS-CoV has shown an RNA molecule of about 29,750 bases in length, with a genome organization similar to that of other coronaviruses ( 9 - 11 ). (cdc.gov)
  • In addition, we have completely sequenced the viral genome of the SARS-CoV HSR1 and compared it to other SARS-CoV strains recently isolated in disease-epidemic areas. (cdc.gov)
  • The human SLC6A19 gene was cloned independently by 2 groups of researchers in 2004. (medscape.com)
  • DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom. (lookformedical.com)
  • of human colon cancer cell lines and and was observed, and STAT3 is critical in MAZ-induced colon tumorigenesis. (healthanddietblog.info)
  • IL-33 can promote survival, adhesion, and cytokine production in human mast cells [ 11 ], but the mechanism behind its involvement in inflammatory conditions remains unclear. (hindawi.com)
  • The human RON gene was originally cloned from keratinocytes ( 1 ). (spandidos-publications.com)
  • Furthermore, other near-human species such as Zeltron, Chiss , and Sullustan share the human ortholog of this locus, which supports the inference that the ancestral sequence was noncoding, and that the GLABR has de novo origins in the Wookiee. (ubc.ca)
  • A complete BLASTP search of all human, and Wookiee proteins from Ensembl (Hubbard et al . (ubc.ca)
  • We analyzed the organization and function of mitochondrial DNA in a stable human cell line (ECV304, which is also known as T-24) containing mitochondria tagged with the yellow fluorescent protein. (biomedcentral.com)
  • We now examine the organization of mitochondrial genomes in a human cell line that contains mitochondria tagged with the yellow fluorescent protein (YFP). (biomedcentral.com)
  • The potential use of embryonic stem cells (ESCs) for cell replacement therapies is limited by ethical concerns and the technical hurdles associated with their isolation from human embryos. (biomedcentral.com)
  • However, the presence of B cells in these individuals suggests important differences between the role of IL-7 in murine and human lymphocyte development. (biolegend.com)
  • Thus, although human B-cell development does not appear to require IL-7, immature human B cells do proliferate in response to IL-7. (biolegend.com)
  • The amino acid sequence of rat IL-7 possesses approximately 89 % and 70 % identity with mouse and human, respectively. (biolegend.com)
  • These results strongly indicate that the Cry1Ia protein and the transgenic potato 'SpuntaG2' is not a human health risk. (ashs.org)
  • Functional recombinant FRβ protein was produced in insect cells and used as antigen to isolate a mAb, m909, from a human naïve Fab phage display library. (biomedcentral.com)
  • AN - check the tag INFANT HN - 2008 FX - Child Nutrition FX - Infant Nutrition Physiology FX - Milk FX - Milk, Human DH - Adolescent Nutrition DI - 052508 MN - SP6.021.067 MS - Nutrition of persons 10 through 19 years of age. (bvsalud.org)
  • epithelial cells of the small intestine and kidney also express these enzymes to a lesser extent, but their contribution to urea production is not significant. (medscape.com)
  • [ 10 , 11 ] In an animal model of Hartnup disorder, mice lacking SLC6A19 (B 0 AT1) transporter general neutral aminoaciduria were observed, as well as the decreased body weight, demonstrating the essential role of epithelial amino acid uptake in optimal growth and bodyweight regulation. (medscape.com)
  • Here we demonstrated the role of DUSP3 in regulation of epithelial TJ. (biomedcentral.com)
  • Therefore, the regulation of cell-cell junctions is a dynamic process during cancer progression, and the regulatory mechanisms are warranted for further studies. (biomedcentral.com)
  • MMR has also been shown to influence cell signaling and the regulation of tumor development. (oncotarget.com)
  • LncSNHG5 was highly expressed in breast CAFs and played an essential role in premetastatic niche formation by promoting angiogenesis and vascular leakiness through regulation of ZNF281 in CAFs. (thno.org)
  • Alternative sites for cell density regulation of the enzyme could rely on its sensitivity to sulfhydryl groups. (embl.de)
  • We propose that lcn2 is an autocrine mediator of reactive astrocytosis based on the multiple roles of lcn2 in the regulation of cell death, morphology, and migration of astrocytes. (jneurosci.org)
  • 2012). This review focuses on the GSI-IX CSF-1R regulation and signaling in cells of the myeloid lineage. (techblessing.com)
  • Hepatitis A virus cellular receptor 1 homolog, HAVcr-1, Kidney injury molecule 1, KIM-1, T cell immunoglobulin and mucin domain-containing protein 1, TIMD-1, T cell membrane protein 1, T-cell immunoglobulin mucin receptor 1, TIM-1. (prospecbio.com)
  • Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. (aspetjournals.org)
  • Ideally, iPSC-based therapies in the future will rely on the isolation of skin fibroblasts or keratinocytes, their reprogramming into iPSCs, and the correction of the genetic defect followed by differentiation into the desired cell type and transplantation. (biomedcentral.com)
  • The complete genomic sequence of the isolate, designated J1 (GenBank accession no. (cdc.gov)
  • The genomic RNA was subjected to RT-PCR amplification followed by sequencing and analysis. (morulaivf.com)
  • In situ hybridization data suggest that KCNE2 transcript may also be expressed in various neuronal populations. (wikipedia.org)
  • The regulatory mechanism of lncSNHG5 was investigated by RNA sequencing, fluorescent in situ hybridization, cellular fractionation assay, mass spectrometry, RNA pull-down, RNA immunoprecipitation, gene-specific m6A assay, chromatin immunoprecipitation, dual luciferase reporter assay and actinomycin D treatment in CAFs and NFs. (thno.org)
  • This GLABR gene was further characterized by a conditional knockout experiment as well as an in situ hybridization on sectioned epithelial cells in order to better determine its function. (ubc.ca)
  • We developed an immunohistochemical (IHC) assay to detect the WUPyV viral protein 1 (WU-VP1) by using an IgG2b designated NN-Ab06. (cdc.gov)
  • Proximity ligation assay, immunoblotting, and phosphatase assay were performed to study the effect of DUSP3 on the TJ protein occludin (OCLN). (biomedcentral.com)
  • ABQ09289) in Escherichia coli from a Gateway pDEST17 plasmid (Life Technologies, Carlsbad, CA, USA) and purifying the protein by using an affinity Ni-NTA column (Pierce Biotechnology, Rockford, IL, USA). (cdc.gov)
  • KCNE proteins contain a single membrane-spanning region, extracellular N-terminal and intracellular C-terminal. (wikipedia.org)
  • The N-terminal domain protrudes out of the extracellular side of the cell membrane and is, thus, soluble in the aqueous environment. (wikipedia.org)
  • While KCNQ1 channels are inhibited by low extracellular pH, KCNQ1-KCNE2 channels activity is augmented by extracellular protons, an ideal characteristic for their role in parietal cells. (wikipedia.org)
  • The extracellular sequences of RON contain several domains including an N-terminal semaphorin (sema) domain, followed by the plexin, semaphorin, integrin (PSI) domain, and four immunoglobulin, plexin, transcription factor (IPT) domains. (spandidos-publications.com)
  • CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. (thermofisher.com)
  • Moreover, cancerous cells tend to cluster together when they are detached from the extracellular matrix. (elifesciences.org)
  • Moreover, PVRL4 triggers a form of signaling between the cells called integrin β4 signaling that allows them to survive without being anchored to the extracellular matrix. (elifesciences.org)
  • More specifically, substrates with certain FYVE domains (named after the first four proteins in this motif, Fab1, YotB, Vac1p, and EEA1) bind PIP, and these pathways have been implicated in vesicular trafficking or receptor-mediated endocytosis ( 5 ). (asm.org)
  • These Wnts were chosen since previous studies suggest that C57mg cells respond differently to these Wnts, and since these Wnts can activate different signaling pathways in other systems. (biomedcentral.com)
  • Sequence homology is an indication of the genetic relatedness of different organisms and gene function. (lookformedical.com)
  • Comparison of the Cry1Ia1 amino acid sequence to known amino acid sequences revealed no significant homology to known toxins or known allergens. (ashs.org)
  • Supporting differences in Wnt signaling, gain of function of Wnt-1 in C57mg mouse mammary epithelial cells leads to their morphological transformation while loss of function of Wnt-5a leads to the same transformation. (biomedcentral.com)
  • the first being a class that transforms C57mg mouse mammary epithelial cells and also promotes duplication of the dorso-ventral axis when over-expressed in Xenopus embryos. (biomedcentral.com)
  • In V3^4-fold increase in transferrin receptor, and a V2-fold transfected cells an association between HFE and TfR occurs increase in iron regulatory protein activity. (healthdrugpdf.com)
  • HeLa cell clones gether with transferrin receptor, indicating a physical association which is the likely cause for the observed V30% decrease in overexpressing HFE showed low levels of endogenous ferritins 55Fe-transferrin incorporation after 18 h incubation. (healthdrugpdf.com)
  • Transferrin receptor tein and by a strong activation of iron responsive element (IRE) binding activity of the iron regulatory proteins (IRPs) which are the typical indices of cellular iron de¢ciency. (healthdrugpdf.com)
  • They found that the gene that codes for a protein called poliovirus receptor-like 4 (PVRL4) allows attachment-free cell growth and also makes cells cluster together once detached from the matrix. (elifesciences.org)
  • This protein belongs to the potassium channel KCNE family and is one five single transmembrane domain voltage-gated potassium (Kv) channel ancillary subunits. (wikipedia.org)
  • Meanwhile, the transmembrane and C-terminal domains are lipid-soluble to enable the protein to incorporate into the cell membrane. (wikipedia.org)
  • TJs, tightly fused regions between the outer membrane of adjacent cells, are formed by integral transmembrane proteins such as OCLN and claudins, anchoring proteins such as zonula occludens (ZO)1-3, and other TJ-associated/regulatory proteins [ 4 , 5 ]. (biomedcentral.com)
  • These motifs contain 2 hydrophobic alpha-helical segments connected by an extensive hydrophilic sequence, resulting in 6 transmembrane portions of the protein. (medscape.com)
  • KCNE2 is also highly expressed in parietal cells of the gastric epithelium, also at the apical side. (wikipedia.org)
  • In invasive LUAD with phosphomimetic FoxM1, IFITM1 is the most highly expressed through the activation of the STING-TBK1-IRF3 signaling, which enhances FoxM1-mediated signaling. (biomedcentral.com)
  • Especially, the potassium channel K V 10.1 (Ether-á-go-go) is attractive as target since this surface protein is virtually not detected in normal tissue outside the central nervous system, but is expressed in approximately 70% of tumors from different origins. (biomedcentral.com)
  • Subcellular fractionation of colon tissue from WT and mice showed the vast majority of MAZ protein in mice was localized to the nucleus (Fig. 1D). (healthanddietblog.info)
  • Epithelial tissue is one of the four major types of tissue found in animals, and is the only type of tissue that is able to form and maintain layers of cells that are just one cell thick. (elifesciences.org)
  • Tissue infiltration by activated innate immune cells, including monocytes, macrophages, and neutrophils, if untreated, leads to rapidly progressive organ injury, that can involve many organ systems. (nature.com)
  • Clones that cross-reacted with KI polyomavirus VP1 (KI-VP1) were identified by ELISA with glutathione S-transferase-tagged KI-VP1 ( 2 ) and eliminated. (cdc.gov)
  • The IgG1 m909 showed much higher (femtomolar) avidity as measured by ELISA, and it bound to FRβ positive cells in a dose-dependent manner, but not to parental FRβ negative cells. (biomedcentral.com)
  • It is conceivable that FRs are useful when folate supply is low or when rapid cell growth requires elevated uptake of folate. (biomedcentral.com)
  • Steve Goldstein (then at Yale University) used a BLAST search strategy, focusing on KCNE1 sequence stretches known to be important for function, to identify related expressed sequence tags (ESTs) in the NCBI database. (wikipedia.org)
  • The clones attained with both primer pieces clustered with archaeal and various other environmental clones deposited at NCBI (Fig. 3), however, not with bacterial 0.01, UniFrac CA-074 Methyl Ester novel inhibtior significance evaluation using Bonferroni correction). (healthweblognews.info)
  • Clones attained with the HAC primer established clustered with NCBI sequences from surface area waters and oxygen minimum amount zones, as the clones acquired with the LAC primer arranged clustered with sequences from oxygen minimum zones and deep waters from a number of regions of the ocean (Fig. 3). (healthweblognews.info)
  • The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. (lookformedical.com)
  • We detected WU polyomavirus (WUPyV) in a bronchoalveolar lavage sample from lungs transplanted into a recipient with Job syndrome by using immunoassays specific for the WUPyV viral protein 1. (cdc.gov)
  • Co-staining for an epithelial cell marker identified most WUPyV viral protein 1-positive cells as respiratory epithelial cells. (cdc.gov)
  • One explanation of the NS1 function is sequestration of viral RNA, which leads to inhibition of main viral RNA sensor RIG-I in the host cell (Chien et al. (datexis.com)
  • US11 has been reported as a potent inhibitor of PKR activation through binding to dsRNA [ 14 ] or through direct interaction with PKR in the context of viral infection [ 12 ] and therefore could interfere with the PKR mediated host cell responses. (biomedcentral.com)
  • To delineate the function of MAZ in the inflammatory progression of colitis, a mouse model of intestinal epithelial cell-specific MAZ-overexpressing transgenic mice was generated. (healthanddietblog.info)
  • Open in another screen FIG 1 Era of intestinal epithelial cell-specific MAZ transgenic mice. (healthanddietblog.info)
  • Our approach, based on reversibly switchable fluorescent proteins, features three distinctly modulated illumination patterns crafted and combined to gain fluorescence ON-OFF switching cycles and image contrast. (nature.com)
  • Using a fluorescent sulfhydryl probe as well as a sulfhydryl inhibitor, one observes a strong cell density response, supporting the hypothesis that cellular redox potential could alter protein stability. (embl.de)
  • E.coli was clearly more appropriate for these kinds of proteins. (europa.eu)
  • The His-tagged US11 protein was then expressed in E. coli BL21 (DE3) cells and purified by a nickel-nitrilotriacetic acid (Ni 2+ -NTA) affinity resin under denaturing conditions. (biomedcentral.com)
  • For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). (prospecbio.com)
  • Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. (biolegend.com)
  • BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. (biolegend.com)
  • As with other mitochondrial carrier family proteins, the ornithine carrier is composed of 300 amino acids that constitute 3 repeated motifs of approximately 100 amino acids each. (medscape.com)
  • The transporter was identified by probing a mammalian-expressed sequence tag database with 2 fungal mitochondrial ornithine carrier protein sequences. (medscape.com)
  • availability and regulates the balance between transferrin- We describe the production of a HeLa cell clone expressing mediated and non-transferrin-mediated cellular iron incorpora- HFE under the control of a tetracycline-inducible promoter. (healthdrugpdf.com)
  • Cell density regulates prolyl 4-hydroxylase activity independent of mRNA levels. (embl.de)
  • In embryonic avian tendon, cell density regulates collagen production. (embl.de)
  • TGF-β signaling is crucial for modulating osteoarthritis (OA), and protein phosphatase magnesium-dependent 1A (PPM1A) has been reported as a phosphatase of SMAD2 and regulates TGF-β signaling, while the role of PPM1A in cartilage homeostasis and OA development remains largely unexplored. (jci.org)
  • HAVCR1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 222 amino acids (22-237 a.a.) and having a molecular mass of 24.4kDa (Migrates at 40-57kDa on SDS-PAGE under reducing conditions). (prospecbio.com)
  • Rat IL-7, amino acids Asp26 - Ile154 (Accession # P56478) was expressed in CHO cells. (biolegend.com)
  • 1988). Another oncogenic derivative is the product of a t(3;5)(p21;q33) translocation RBM6-CSF-1R a constitutively activated CSF-1R fusion protein comprised of the amino-terminal 36 amino acids of RNA-binding motif 6 (RBM6) joined to the carboxy-terminal 399 amino acids of the CSF-1R which leads to an acute megakaryoblastic leukemia (Fig. 1A). (techblessing.com)
  • The C-terminal faces the intracellular side of the membrane and may share a putative PKC phosphorylation site with other KCNE proteins. (wikipedia.org)
  • TRAIL is normally present as a membrane protein (memTRAIL) on immune effector cells, like natural killer cells. (biomedcentral.com)
  • This mitochondrial RNA colocalizes with components of the cytoplasmic machinery that makes and imports nuclear-encoded proteins - that is, a ribosomal protein (S6), a nascent peptide associated protein (NAC), and the translocase in the outer membrane (Tom22). (biomedcentral.com)
  • Ultrastructural analysis of infected Vero cells showed the virions within cell vesicles and around the cell membrane. (cdc.gov)