• cleavage
  • This enzyme catalyses the following chemical reaction: Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates Preference for[clarification needed] single-stranded DNA. (wikipedia.org)
  • This enzyme catalyses the following chemical reaction Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products Although its primary substrate is single-stranded, it can also occasionally introduce single-stranded breaks in double-stranded DNA or RNA, or DNA-RNA hybrids. (wikipedia.org)
  • biomass
  • This study of fungi that bioconvert energy crops is significant because 30 fungi were studied, because the fungi were isolated from decaying energy grasses, because enzyme activity and removal of plant cell wall components were recorded in addition to biomass conversion, and because the study period was 2 months. (biomedcentral.com)
  • However, the major obstacle in the use of lignocellulosic biomass is its recalcitrant nature, which decreases the enzyme accessibility to cellulose and thus affects the overall hydrolysis process. (sun.ac.za)
  • produce enzymes
  • We, and others [ 4 , 10 ], have reasoned that fungi that naturally deconstruct the cell walls of sugarcane and Miscanthus might produce enzymes with the diversity and strength of activity best suited to bioconversion of these plants. (biomedcentral.com)
  • catalyses
  • This enzyme catalyses the following chemical reaction 2-hydroxy-dATP + H2O ⇌ {\displaystyle \rightleftharpoons } 2-hydroxy-dAMP + diphosphate The enzyme hydrolyses oxidizes purine nucleoside triphosphates. (wikipedia.org)
  • detergent
  • Technical enzymes for detergent, paper & pulp, and textile manufacturing have the largest segment with approximately 52% market share with the largest share of the enzymes market held by alkaline proteases. (reportsnreports.com)
  • driven
  • The evolution was driven by iterative saturation mutagenesis in combination with enzyme activity screenings where product chirality was the decisive selection criterion. (diva-portal.org)
  • ethanol
  • The maximum ethanol yield produced by S. cerevisiae S2[TLG, SFA] and S. cerevisiae MH1000[TLG, SFA], in the presence of the 1x enzyme cocktail, was 5.72 g.l-1 and 5.45 g.l-1, respectively. (sun.ac.za)
  • bioethanol
  • The development of enzyme cocktails for efficient hydrolysis of paper sludge is therefore important in the pursuit of second-generation bioethanol production. (sun.ac.za)
  • Glycoside
  • Glycoside hydrolase enzymes hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. (wikipedia.org)
  • catalytic
  • Carbonyl oxygen of the C-2 acetamido group of the substrate acts as the catalytic nucleophile/base in this family of enzymes. (wikipedia.org)
  • As noted earlier, the Nudix fold is the catalytic part of the enzyme, but it should also be noted that both domains are involved in the active site and they both help with the attachment and coordination of H2O, Mg2+, and the ADP-ribose substrate. (wikipedia.org)
  • With the substrate bound, Loop L9 moves 10 angstroms from its original position in the free enzyme, which forms a tighter turn and brings E162 to its catalytic position, meaning this enzyme cycles between an open (free enzyme) and a closed (substrate-metal complex) conformation. (wikipedia.org)
  • The debate of whether enterokinase was a cofactor or enzyme was resolved by Kunitz, who showed that the activation of trypsinogen by enterokinase was catalytic. (wikipedia.org)
  • Classification
  • This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site, and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes. (wikipedia.org)
  • starch
  • In addition to cost, enzyme diversity is an issue because the plant cell wall, with its many polysaccharides, is far more complex than starch. (biomedcentral.com)
  • Glucose syrup, also known as confectioner's glucose, is a syrup made from the hydrolysis of starch. (wikipedia.org)
  • Depending on the method used to hydrolyse the starch and on the extent to which the hydrolysis reaction has been allowed to proceed, different grades of glucose syrup are produced, which have different characteristics and uses. (wikipedia.org)
  • Maltose is also less humectant than glucose, so candy produced with high-maltose syrup will not become sticky as easily as candy produced with a standard glucose syrup.p. 81 Irrespective of the feedstock or the method used for hydrolysis, certain steps are common to the production of glucose syrup: Before conversion of starch to glucose can begin, the starch must be separated from the plant material. (wikipedia.org)
  • This prepares the starch for hydrolysis. (wikipedia.org)
  • Currently, glucose syrup is mainly produced by first adding the enzyme α-amylase to a mixture of corn starch and water. (wikipedia.org)
  • reaction
  • For biocatalytic purpose the enzyme were was successfully immobilized on alumina oxide membranes to function in a two-step biocatalytic reaction with immobilized alcoholdehydrogenase A from Rhodococcus ruber , producing 2-hydroxyacetophenone from racemic 2 . (diva-portal.org)
  • A uniform design coupled with subset selection was employed to determine pH, reaction time, and temperature parameters, as well as dextranase hydrolysis efficiency. (dicp.ac.cn)
  • Our results showed that pH plays an important role in the hydrolysis reaction within a relatively narrow range (pH 4-7). (dicp.ac.cn)
  • ADP-ribose diphosphatase (EC 3.6.1.13) is an enzyme that catalyzes a hydrolysis reaction in which water nucleophilically attacks ADP-ribose to produce AMP and D-ribose 5-phosphate. (wikipedia.org)
  • bacteria
  • the enzyme is isolated from the liquid in which the bacteria are grown. (wikipedia.org)
  • The glucose can then be transformed into fructose by passing the glucose through a column that is loaded with the enzyme D-xylose isomerase, an enzyme that is isolated from the growth medium of any of several bacteria. (wikipedia.org)
  • catalyzes
  • Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond of lysoplasmalogen. (nih.gov)
  • relation
  • The relation between enzyme activity and the virulence and invasiveness of group A hemolytic streptococci is briefly considered. (rupress.org)
  • Plants
  • Here, we thoroughly analyze 30 of these fungi including those most often found on decaying leaves and stems of these plants, as well as four fungi chosen because they are well-studied for their plant cell wall deconstructing enzymes, for wood decay, or for genetic regulation of plant cell wall deconstruction. (biomedcentral.com)
  • activity
  • Fractions were tested for lysoplasmalogenase activity ( black filled circles ) using the coupled enzyme assay and were expressed in nmol/min/fraction. (nih.gov)
  • The inert function of digestive enzymes within the pancreas was known, as compared to their potent activity within the intestine, but the basis of this difference was unknown. (wikipedia.org)
  • In 1899, Pavlov's student, N. P. Schepowalnikov, demonstrated that canine duodenal secretions dramatically stimulated the digestive activity of pancreatic enzymes, especially trypsinogen. (wikipedia.org)
  • Thus
  • Thus, variant enzymes that catalyzed the hydrolysis of racemic (2,3-epoxypropyl)benzene into the R -diol product in an enantioconvergent manner were isolated. (diva-portal.org)
  • Thus, it is believed that ADPRase functions in general as a house-cleaning enzyme to eliminate potentially deleterious ADP-ribose from the cell. (wikipedia.org)