• cleavage
  • Lysoplasmalogenase (EC 3.3.2.2 and EC 3.3.2.5) is an enzyme that catalyzes hydrolytic cleavage of the vinyl ether bond of lysoplasmalogen, forming fatty aldehyde and glycerophosphoethanolamine or glycerophosphocholine and is specific for the sn-2-deacylated form of plasmalogen. (nih.gov)
  • This enzyme catalyses the following chemical reaction: Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates Preference for[clarification needed] single-stranded DNA. (wikipedia.org)
  • This enzyme catalyses the following chemical reaction Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products Although its primary substrate is single-stranded, it can also occasionally introduce single-stranded breaks in double-stranded DNA or RNA, or DNA-RNA hybrids. (wikipedia.org)
  • These enzymes are activated by cleavage at the carboxyl side of lysine or arginine residues present in a highly conserved activation motif. (wikipedia.org)
  • biomass
  • This study of fungi that bioconvert energy crops is significant because 30 fungi were studied, because the fungi were isolated from decaying energy grasses, because enzyme activity and removal of plant cell wall components were recorded in addition to biomass conversion, and because the study period was 2 months. (biomedcentral.com)
  • However, the major obstacle in the use of lignocellulosic biomass is its recalcitrant nature, which decreases the enzyme accessibility to cellulose and thus affects the overall hydrolysis process. (sun.ac.za)
  • Commercialization of lignocellulosic biomass as a feedstock for bio-based chemical production is problematic due to the high processing costs of pretreatment and saccharifying enzymes combined with low product yields. (springer.com)
  • linkages
  • There are four type of linkages present between glycone and aglycone: C-linkage/glycosidic bond, "nonhydrolysable by acids or enzymes" O-linkage/glycosidic bond N-linkage/glycosidic bond S-linkage/glycosidic bond Glycosides are also classified according to the chemical nature of the aglycone. (wikipedia.org)
  • This enzyme catalyses the following chemical reaction Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen This enzyme hydrolyses an unsubstituted (1->4)-linked glucose chain. (wikipedia.org)
  • driven
  • The evolution was driven by iterative saturation mutagenesis in combination with enzyme activity screenings where product chirality was the decisive selection criterion. (diva-portal.org)
  • ethanol
  • The maximum ethanol yield produced by S. cerevisiae S2[TLG, SFA] and S. cerevisiae MH1000[TLG, SFA], in the presence of the 1x enzyme cocktail, was 5.72 g.l-1 and 5.45 g.l-1, respectively. (sun.ac.za)
  • bioethanol
  • The development of enzyme cocktails for efficient hydrolysis of paper sludge is therefore important in the pursuit of second-generation bioethanol production. (sun.ac.za)
  • catalytic
  • Carbonyl oxygen of the C-2 acetamido group of the substrate acts as the catalytic nucleophile/base in this family of enzymes. (wikipedia.org)
  • As noted earlier, the Nudix fold is the catalytic part of the enzyme, but it should also be noted that both domains are involved in the active site and they both help with the attachment and coordination of H2O, Mg2+, and the ADP-ribose substrate. (wikipedia.org)
  • With the substrate bound, Loop L9 moves 10 angstroms from its original position in the free enzyme, which forms a tighter turn and brings E162 to its catalytic position, meaning this enzyme cycles between an open (free enzyme) and a closed (substrate-metal complex) conformation. (wikipedia.org)
  • The debate of whether enterokinase was a cofactor or enzyme was resolved by Kunitz, who showed that the activation of trypsinogen by enterokinase was catalytic. (wikipedia.org)
  • glycogen
  • This is a key enzyme in glycogen metabolism and its gene localizes to a chromosomal region (15q15) that is associated with susceptibility to diabetes. (wikipedia.org)
  • systematic name
  • 2-hydroxy-dATP diphosphatase (EC 3.6.1.56, NUDT1, MTH1, MTH2, oxidized purine nucleoside triphosphatase, (2'-deoxy) ribonucleoside 5'-triphosphate pyrophosphohydrolase) is an enzyme with systematic name 2-hydroxy-dATP diphosphohydrolase. (wikipedia.org)
  • starch
  • In addition to cost, enzyme diversity is an issue because the plant cell wall, with its many polysaccharides, is far more complex than starch. (biomedcentral.com)
  • Glucose syrup, also known as confectioner's glucose, is a syrup made from the hydrolysis of starch. (wikipedia.org)
  • Depending on the method used to hydrolyse the starch and on the extent to which the hydrolysis reaction has been allowed to proceed, different grades of glucose syrup are produced, which have different characteristics and uses. (wikipedia.org)
  • Maltose is also less humectant than glucose, so candy produced with high-maltose syrup will not become sticky as easily as candy produced with a standard glucose syrup.p. 81 Irrespective of the feedstock or the method used for hydrolysis, certain steps are common to the production of glucose syrup: Before conversion of starch to glucose can begin, the starch must be separated from the plant material. (wikipedia.org)
  • This prepares the starch for hydrolysis. (wikipedia.org)
  • Currently, glucose syrup is mainly produced by first adding the enzyme α-amylase to a mixture of corn starch and water. (wikipedia.org)
  • reaction
  • For biocatalytic purpose the enzyme were was successfully immobilized on alumina oxide membranes to function in a two-step biocatalytic reaction with immobilized alcoholdehydrogenase A from Rhodococcus ruber , producing 2-hydroxyacetophenone from racemic 2 . (diva-portal.org)
  • A uniform design coupled with subset selection was employed to determine pH, reaction time, and temperature parameters, as well as dextranase hydrolysis efficiency. (dicp.ac.cn)
  • Our results showed that pH plays an important role in the hydrolysis reaction within a relatively narrow range (pH 4-7). (dicp.ac.cn)
  • ADP-ribose diphosphatase (EC 3.6.1.13) is an enzyme that catalyzes a hydrolysis reaction in which water nucleophilically attacks ADP-ribose to produce AMP and D-ribose 5-phosphate. (wikipedia.org)
  • glucose
  • The glucose can then be transformed into fructose by passing the glucose through a column that is loaded with the enzyme D-xylose isomerase, an enzyme that is isolated from the growth medium of any of several bacteria. (wikipedia.org)
  • catalyzes
  • Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond of lysoplasmalogen. (nih.gov)
  • relation
  • The relation between enzyme activity and the virulence and invasiveness of group A hemolytic streptococci is briefly considered. (rupress.org)
  • activity
  • Fractions were tested for lysoplasmalogenase activity ( black filled circles ) using the coupled enzyme assay and were expressed in nmol/min/fraction. (nih.gov)
  • We, and others [ 4 , 10 ], have reasoned that fungi that naturally deconstruct the cell walls of sugarcane and Miscanthus might produce enzymes with the diversity and strength of activity best suited to bioconversion of these plants. (biomedcentral.com)
  • The inert function of digestive enzymes within the pancreas was known, as compared to their potent activity within the intestine, but the basis of this difference was unknown. (wikipedia.org)
  • In 1899, Pavlov's student, N. P. Schepowalnikov, demonstrated that canine duodenal secretions dramatically stimulated the digestive activity of pancreatic enzymes, especially trypsinogen. (wikipedia.org)
  • Thus
  • Thus, variant enzymes that catalyzed the hydrolysis of racemic (2,3-epoxypropyl)benzene into the R -diol product in an enantioconvergent manner were isolated. (diva-portal.org)
  • Thus, it is believed that ADPRase functions in general as a house-cleaning enzyme to eliminate potentially deleterious ADP-ribose from the cell. (wikipedia.org)
  • important
  • Overexpression of TMEM86b in HEK 293T cells resulted in decreased levels of plasmalogens, suggesting that the enzyme may be important in regulating plasmalogen levels in animal cells. (nih.gov)
  • This is important because it prevents nonspecific hydrolysis of other nucleotides. (wikipedia.org)
  • molecular
  • Baohuoside I production through enzyme hydrolysis and parameter optimization by using response surface and subset selection[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2013,90:132-138. (dicp.ac.cn)
  • The DNA is digested by a particular restriction enzyme, resulting in DNA pieces of varying molecular masses. (wikipedia.org)
  • bound
  • In pneumococci and hemolytic streptococci the major portion of the enzyme is bound to the cell structure. (rupress.org)
  • When the substrate is bound, the structure of the enzyme differs in shape. (wikipedia.org)
  • Growth
  • The wider scope of commercial application of protein hydrolysate enzymes is driving the growth. (researchbeam.com)
  • Increase in demand for environment friendly chemical additives and low cost labeling solution are promoting the growth of Protein hydrolysate enzymes. (researchbeam.com)
  • Regulations form respective geographies and availability of raw materials are hindering the growth of Protein hydrolysate enzymes market. (researchbeam.com)
  • method
  • The values of these constants for benzoyl L-tyrosinhydrazide have been obtained by the method of competitive hydrolysis. (caltech.edu)
  • The second method employs the use of restriction enzymes and a recognized DNA sequence. (wikipedia.org)