Enzymes found activity in subcellular fractions of different. Medical search. Frequent questions

An additional 1.5-fold enhancement was observed in the cytosolic fraction from PB-treated rats. (aspetjournals.org)

Quantitatively, GSH conjugates formed in the cytosolic fraction were 23-fold that of control. (aspetjournals.org)
Phosphoramidon-inhibitable converting activity was also detected in the cytosolic fraction of BAE. (aspetjournals.org)

[1] pl. mitochondria ) is an organelle found in the cells of most eukaryotes , such as animals , plants and fungi . (wikipedia.org)
[20] This finding has led to general acceptance of the endosymbiotic hypothesis - that free-living prokaryotic ancestors of modern mitochondria permanently fused with eukaryotic cells in the distant past, evolving such that modern animals, plants, fungi, and other eukaryotes are able to respire to generate cellular energy . (wikipedia.org)
By tinkering with the speed and order of spins with a centrifuge, Claude could thus physically separate known subcellular structures into four rough fractions: large and heavy nuclei, medium-sized mitochondria, a smaller fraction he eventually termed "microsomes," and the freely soluble cytoplasm. (rockefeller.edu)
About 30% of the homogenate activity after the removal of the nuclear fraction was recovered in the peroxisomes, about 56% in the mitochondria, and the remainder in the soluble fraction from broken peroxisomes. (unipr.it)
Glucagon injection to rats resulted in the increase of its activity in the mitochondria but not in the peroxisomes. (unipr.it)
The ATPase activity (or activities) in this aerotolerant anaerobe is different from the ATPases characteristic of mitochondria or of anaerobic bacteria. (docksci.com)
In our earlier reports, it was demonstrated that experimentally hypothyroidism modulates several oxidative stress and antioxidant defence parameters in mitochondria and postmitochondrial fractions in adult rat testis [ 18 , 19 ]. (hindawi.com)

In addition, a sex difference in hepatic microsomal TRI metabolism, specific CYP content, and ethoxyresorufin O-deethylase activity was noted. (cdc.gov)
RapidStart™ is a versatile system that supports extended metabolism of test articles by NADPH-dependent drug-metabolizing enzymes in subcellular fractions for in vitro drug metabolism assays. (tebubio.com)
Assays using microsomes, S9 and recombinant CYPs (Bactosomes) can be supported with RapidStart™ to support the long-term metabolism of test articles by these enzymes by ensuring that NADPH is not the limiting reagent. (tebubio.com)
These subcellular fractions are helpful to researchers in predicting the hepatic and extrahepatic metabolism of drug compounds to understand their absorption, distribution, metabolism, and excretion profile. (kosheeka.com)
Out of many subcellular fractions used regularly in a preclinical research setting, microsomes contain a cocktail of most enzymes involved in drug metabolism, including transporter enzymes like cytochrome P450. (kosheeka.com)
The subcellular fractions from various strains of rats are recommended for use in drug development and understanding cellular metabolic pathways as predictive test systems to evaluate the route of metabolism of drug components in the liver and in extra-hepatic tissues. (kosheeka.com)
Because a decreased dependence on aerobic respiration becomes advantageous, cell metabolism is shifted toward glycolysis by the increased expression of glycolytic enzymes, glucose transporters, and inhibitors of mitochondrial metabolism. (pharmaceuticalintelligence.com)

The lower specific activity of preparations from the livers of female medaka is compensated for by increased total protein in the larger liver mass of the female. (cdc.gov)
The VEGF-A protein and mRNA levels in conditioned medium of RGC-5 cells incubated with AGE-modified BSA (AGE-BSA) were examined with real-time PCR and enzyme-linked immunosorbent assay (ELISA), and BSA-treated cells were used as controls. (molvis.org)
Here I pursued my scientific interests in subcellular protein localisation, bioinformatics, and mathematical modelling. (brookes.ac.uk)
Moreover, although the abundance of most protein groups reflected that of related bacterial populations, we found a specific independent regulation of bacteria-derived cell envelope proteins. (bmj.com)
The latter but not the former activity was inhibited in a concentration-dependent manner by phosphoramidon (approximate IC50, 1 microM) and converted bET-1 to ET-1 at a rate of 0.6 nmol/hr/mg of protein. (aspetjournals.org)
To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. (jci.org)
Short-term activation of protein kinase C by 5 min exposure to PMA caused an acute increase in Na/H antiporter activity that was not prevented by cycloheximide or actinomycin D and did not persist 24 h later. (jci.org)
Long-term activation of protein kinase C by 2 h exposure to PMA caused a dose-dependent increase in Na/H antiporter activity 24 h later. (jci.org)
In conclusion, short-term activation of protein kinase C leads to a transient increase in Na/H antiporter activity that is independent of transcription and translation, whereas long-term activation of protein kinase C causes a persistent increase in antiporter activity that is dependent on transcription and translation and is associated with increased mRNANa/H abundance. (jci.org)
An altered antioxidant defence system marked by elevated SOD, CAT, and GR activities, with decreased GPx and GST activities were observed along with increased protein carbonylation, disturbed redox status in hypothyroid immature rat testis. (hindawi.com)
Here we show by mass spectrometric analyses of enriched insoluble protein fractions that, unexpectedly, nucleus-encoded transporters are not inserted into the chromatophore inner envelope membrane. (frontiersin.org)
Additional protein domains found at their C termini have been used to classify TRIM proteins into subfamilies. (elifesciences.org)

In this study, we have used indirect immunofluorescence microscopy and cell fractionation to study the subcellular distribution of all three subunits of chicken CK II, alpha, alpha', and beta. (rupress.org)
The demand for pure cellular fractions has prompted the development of novel methods, allowing participants in the cell fractionation market to increase their options. (marketdataforecast.com)
This review will evaluate the subcellular fractionation protocols and the proteomic approaches employed. (mdpi.com)
Preparation of cell extracts and subcellular fractionation. (docksci.com)

antibody response to the 14-valent pneumococcal capsular polysaccharide vaccine was measured by the enzyme-linked immunosorbent assay (eia) in 17 renal allograft recipients, 29 azotemic, 11 hemodialysis, and 33 control patients. (liverpool.ac.uk)

Nevertheless, quercetin is completely converted into its conjugated metabolites by phase-II enzymes during intestinal absorption. (rsc.org)
Phase II enzymes present in the intestinal epithelial cells and the liver cells facilitate their metabolic conversion to glucuronide and sulfate conjugates. (rsc.org)
At the same time, S9 fractions are a mix of cytosolic and microsomal enzymes, mostly expressing a number of phases I and phase II enzymes. (kosheeka.com)

In line with these results, studies using various enzyme inhibitors, such as DPI, allopurinol, rotenone, N G -monomethyl- l -arginine, and indomethacin, suggest that the predominant source of superoxide anion in vascular particulate fraction is NADH-dependent membrane-bound oxidase. (diabetesjournals.org)
Within the particulate fraction of BAE, we detected two activities, at pH 3.4 and pH 5.4-7.4. (aspetjournals.org)
It could be solubilized from the particulate fraction by detergent treatment. (aspetjournals.org)

It is likely to act as a bioactive compound by exerting reactive oxygen species (ROS)-scavenging activity and/or binding to specific proteins such as oxidative enzymes and transcriptional factors in signal transduction pathways. (rsc.org)
A crucial development in this area is the requirement to isolate subcellular proteins from cultivated cells. (marketdataforecast.com)
a technique of derivatization of proline (pro) and 4-hydroxyproline (hyp) by 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole permitted the measurement of pro and hyp radioactivities, concentrations, and specific activities in the main fractions separated from cultures of fibroblast cells (extracellular collagen and non-collagen proteins, intracellular free pro and hyp, pro- and hyp-containing peptides, procollagen, and non-collagen proteins). (liverpool.ac.uk)
Gel filtration chromatography of 'P' tissue in Sephacryl S-300 shows that the reactivated activity is associated with proteins of about 100-kDa mass which include S-NTE and an, as yet, unknown number of other PVases. (inrae.fr)

Soluble extracts of chicken peripheral nerve contain detectable amounts of phenyl valerate esterase (PVase) activity (about 2000 nmol/min per g of fresh tissue). (inrae.fr)
More than 95% of this activity is inhibited in assays where substrate has been added to a preincubated mixture of tissue with the non-neuropathic organophosphorus compound (OP) paraoxon (O,O'-diethyl p-nitrophenyl phosphate): residual activity includes soluble neuropathy target esterase (S-NTE) which, by definition, is considered resistant to long-term progressive (covalent) inhibition by paraoxon. (inrae.fr)
We now show that, surprisingly, removal of paraoxon by ultrafiltration ('P' tissue) in order to avoid such an interference results in the reappearance of about 65% of total original soluble PVase activity which is inhibited in the presence of this OP. Although a purely reversible non-progressive inhibition might be suspected, kinetic analysis data show a time-progressive inhibition which suggests that such PVase(s) covalently bind paraoxon. (inrae.fr)
He localized the biologic activity to a fraction of lipid soluble acids that he termed "prostaglandin," hypothesizing that these substances originate in the prostate gland. (medscape.com)

Conversion of TRI to CH, TCA, and TCOH was found with medaka hepatic subcellular fractions. (cdc.gov)
In this study, GST activities were detected in the hepatic cytosolic and sonic-disrupted mitoplast fractions isolated from male Sprague-Dawley rats by using 1-chloro-2,4-dinitrobenzene as a substrate. (aspetjournals.org)

Hexokinase (HXK) is a glycolytic regulatory enzyme that catalyzes the irreversible phosphorylation reaction of D-hexoses at the sixth carbon using ATP-Mg 2+ as a phosphate donor. (biomedcentral.com)
Regardless of the etiology, a deficiency of cyclooxygenase (COX), a key regulatory enzyme in the synthetic pathway of eicosanoid production, results in beneficial and detrimental physiologic conditions relative to imbalances of the eicosanoids. (medscape.com)

Hexokinase (HXK) is the only plant enzyme that phosphorylates glucose (Glc), so it is key to fueling several metabolic pathways depending on their substrate specificity, metabolite regulatory responses and subcellular localization. (biomedcentral.com)

Based on this work I won a fellowship from the Korean Federation of Science and Technology Societies to investigate the subcellular localisation of maize auxin biosynthesis at Dankook University in Seoul which lead to a publication that showed for the first time that both steps of the TAA/YUC pathway of auxin biosynthesis can be ER-localised. (brookes.ac.uk)
In maize, the HXK family is composed of nine genes, but only six of them (ZmHXK4-9) putatively encode catalytically active enzymes. (biomedcentral.com)

Cytochrome P450 (CYP) and other enzymes are responsible for formation of these metabolites, which are implicated in TRI's toxicity and carcinogenicity. (cdc.gov)
NADPH is a cofactor required to support reactions by drug-metabolizing enzymes such as cytochrome P450 (CYP) and flavin-containing monooxygenases (FMO). (tebubio.com)

Find our optimized NADPH regeneration system and catabolism buffer to pair with your in vitro assays using recombinant enzymes and subcellular fractions. (tebubio.com)
Today, many preclinical research domains depend upon cell-based in vitro assays requiring either primary cells/cell-based models or subcellular fractions isolated from various species to analyze drug metabolic activities. (kosheeka.com)

Only the 1,6- addition product was found for the spontaneous conjugation reaction (control). (aspetjournals.org)
These results indicate that in vivo production of the GSH conjugates of (E)-2,4-diene VPA is most likely catalyzed by GST enzymes, with the esterified diene being essential for the conjugation reaction. (aspetjournals.org)
The kinetic profile of K-pNPPase and its sensitivity toward ouabain and vanadate confirmed the identification of this activity with the partial reaction of the sodium pump. (mcmaster.ca)
Their distinct biosynthetic activity includes an endoperoxidase synthase reaction that oxygenates and cyclizes polyunsaturated fatty acid precursors (eg, arachidonic acid) to form prostaglandin G 2 (PGG2), and a peroxidase reaction that converts PGG2 to prostaglandin H 2 (PGH2), as shown below. (medscape.com)

We discovered that a conjugated metabolite derived from quercetin possesses considerable anti-oxidant activity in several in vitro experiments. (rsc.org)

Alkyltin Inhibition of ATPase Activities in Tissue Homogenates and Subcellular Fractions from Adult and Neonatal Rats (Journal Version). (epa.gov)
An elevation of GST activities by 45 to 100% was observed after pretreatment of rats with phenobarbital (PB). (aspetjournals.org)
Systemic inflammatory parameters such as myeloperoxidase activity in lung was found to be significantly lower in the inhibitor fed rats. (uni-greifswald.de)
These findings suggest that upregulated expression of p22phox mRNA and enhanced NADH oxidase activity contribute to the impaired endothelium-dependent vasodilation in OLETF rats. (diabetesjournals.org)

Inhibition of ATPase Activities of Brain and Liver Homogenates by Triethyltin (TET). (epa.gov)
Since pigs have been reported to be among the most sensitive animal species, biotransformation studies with pig liver subcellular fractions were conducted. (vetres.org)
The subcellular distribution of asparagine:oxo-acid aminotransferase (EC 2.6.1.14) in rat liver was examined by centrifugation in a sucrose density gradient. (unipr.it)
All of alanine:glyoxylate aminotransferase 1 in livers of mammals other than rodents, which cross-react with the antibody against rat liver alanine:glyoxylate aminotransferase 1, had no asparagine aminotransferase activity. (unipr.it)

Subcellular fractions like S9, cytosol, and microsomes obtained from tissues of different animals of choice can be used as a suitable test system to understand drug development metabolic pathways. (kosheeka.com)
We also offer pooled/single donor fractions of S9, microsomes, and cytosols from non-human primates like monkeys, dogs, etc. (kosheeka.com)

Objective Indole‐3‐carbinol (I3C), a naturally occurring compound found in cruciferous vegetables, and its metabolite 3,3′‐diindolylmethane (DIM) reduce body mass and serum glucose levels in high‐fat‐diet‐induced obese mice. (researchgate.net)

The key to the PARP1 Colorimetric Activity Assay is the biotinylated substrate. (tebubio.com)
Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. (tebubio.com)
The key to the PARP2 Colorimetric Activity Assay is the biotinylated substrate. (tebubio.com)
Next, the PARP2 biotinylated substrate is incubated with an assay buffer that contains the PARP2 enzyme. (tebubio.com)
Substrate specific trypsin activity and western blotting from both sub-cellular fractions over the time course of pancreatitis and multiple fractions prepared from 1 h caerulein induced pancreatic tissue revealed that trypsin activity observed at 1 h occured in a zymogen enriched fraction. (uni-greifswald.de)

Food-grade titanium dioxide (TiO 2 ) containing a nanoscale particle fraction (TiO 2 -NPs) is approved as a white pigment (E171 in Europe) in common foodstuffs, including confectionary. (nature.com)

These results strongly suggest that CK II functions primarily in regulating nuclear activities, and that the two catalytic subunits, alpha and alpha', may carry out overlapping functions. (rupress.org)
Here, we cloned and functionally characterized putative catalytic enzymes to analyze their metabolic contribution during germination process. (biomedcentral.com)

The lowest levels of tissue oxygenation were found in the pectoral muscle fibres of the icefish Chaenocephalus aceratus , which lacks the respiratory pigments haemoglobin and myoglobin. (biologists.com)
It is described as a housekeeping enzyme, being responsible for cell-to-cell signaling, tissue homeostasis, and cytoprotection. (medscape.com)

A pair of principal enzymes, glutamate dehydrogenase and glutamine synthatase, are found in all organisms and effect the conversion of ammonia into the amino acids glutamate and glutamine, respectively. (medmuv.com)
Since adenosine triphosphatases (ATPases) of different organelles and micro-organisms show characteristic properties, it was felt that a study of the ATPase activities present in a trichomonad could contribute to the elucidation of the biological nature of hydrogenosomes. (docksci.com)

HXK activity was detected in cytosolic and mitochondrial fractions and high Glc and Fru phosphorylating activities were found in imbibed embryos. (biomedcentral.com)
Considering the biochemical characteristics, location and the expression of ZmHXK4 at onset of germination, we suggest that it is the main contributor to mitochondrial activity at early germination times, at 24 h other ZmHXKs also contribute to the total activity. (biomedcentral.com)

Abstract - Zearalenone (ZEA) is a resorcylic acid lactone derivative produced by various Fusarium species that are widely found in food and feeds. (vetres.org)

Casein kinase II is a predominantly nuclear enzyme. (rupress.org)

These results indicate that the two intestinal secretory peptides antagonistically regulate adult lifespan and intestinal senescence through multiple pathways, irrespective of insulin, which implicates a complementary gradient distribution of each of the hormone-producing EEs, consistent with local requirements for cell activity along the posterior midgut. (bioone.org)

No major differences were detected in the subcellular distributions of individual CK II subunits, and no evidence was obtained for subunit redistributions during interphase of the cell cycle. (rupress.org)
Immunological evidence was obtained that the enzyme was identical with alanine:glyoxylate aminotransferase 1 (EC 2.6.1.44) which had been reported to be identical with serine:pyruvate aminotransferase (EC 2.6.1.51) (Noguchi, T. (1987) in Peroxisomes in Biology and Medicine (Fahimi, H. D., and Sies, H., eds) pp. 234-243, Springer-Verlag, Heidelberg). (unipr.it)

14,15 Conversely, its pro-oxidant activity may enhance cellular defence against oxidants by stimulating the redox-regulated signal transduction pathway which leads to the gene expression of anti-oxidative enzymes such as heme oxygenase-1 (HO-1) and glutathione peroxidase (GPx). (rsc.org)
Therefore, the dramatic changes of sugar type and concentration during the germination process undoubtedly regulates the enzyme activity and the pattern of gene expression of several enzymes [ 5 , 6 ]. (biomedcentral.com)

Characterization of endothelin-converting enzyme from endothelial cells and rat brain: detection of the formation of biologically active endothelin-1 by rapid bioassay. (aspetjournals.org)

Here, using measurements of endogenous Piezo1 activity and traction forces in native cellular conditions, we show that cellular traction forces generate spatially-restricted Piezo1-mediated Ca 2+ flickers in the absence of externally-applied mechanical forces. (nature.com)
Due to their permeability to Ca 2+ and other cations, Piezo channel activity generates chemical as well as electrical signals in response to mechanical stimuli, allowing them to regulate a wide variety of cellular processes. (nature.com)

4-Nitrophenol and the alkyl phosphate, dimethyl phosphate, are major metabolites that are frequently found in biological fluids and tissues following exposure. (cdc.gov)

3,3′-diindolylmethane (DIM)-a natural compound produced from indole-3-carbinol, found in cruciferous vegetables-enhances glucose uptake by increasing the activation of the insulin signaling pathway in 3T3-L1 adipocytes. (researchgate.net)

For the extraction of water from fabrics, medium-sized centrifuges can be found in washing machines as well as at swimming pools. (microbiologynote.com)

No inhibitor binding sites characteristic of mitochondria1ATPase nor the presence of an ATPase similar to that found in Clostridium pasteurianum (Clarke & Morris, 1976, 1977) could be detected. (docksci.com)

This polyphenolic compound is known to be a strong anti-oxidant which scavenges reactive oxygen species (ROS) directly or effectively inhibits ROS-generating enzymes ( e.g. xanthine oxidase, lipoxygenase). (rsc.org)
If you are seeking subcellular fractions from any particular tox species, you have reached the right place. (kosheeka.com)

Perturbations in the Biomass, Metabolic Activity, and Community Structure of the Estuarine Detrital Microbota: Resource Partitioning in Amphipod Grazing. (epa.gov)
Seed germination, which starts with the imbibition, is a crucial event in the plant life cycle when metabolic activity resumes, and reserves are mobilized to support initial plant development. (biomedcentral.com)

Clinical symptoms include elevated levels (minimum 3 times than normal) of pancreatic enzymes such as amylase or lipase in serum. (uni-greifswald.de)

Even though, autophagosomes and active trypsin were found to be co-localised around 30 minutes to some degree upon cholecystokinin hyperstimulation, the earliest trypsin activation started to appear by 15 minutes and was independent of autophagosomes. (uni-greifswald.de)
The earliest active trypsin was found to be co-localised along with the cis-Golgi complex suggesting that the Golgi apparatus and its pre-condensed zymogen granules are the compartment responsible for the trypsinogen activation. (uni-greifswald.de)
Previous studies have found that DIM can improve type 2 diabetes by enhancing glucose uptake through the activation of insulin signaling in 3T3-L1 cells, and by lowering the plasma glucose levels in high-fat-diet-fed obese mice [13, 14]. (researchgate.net)

Dose-dependent increase in glucose uptake activity (GUA) was observed in both cell lines. (wiley.com)

To better understand the function(s) and regulation of this ubiquitous kinase, it is important to know its subcellular distribution. (rupress.org)
AEG was found to enhance glycogen synthesis through the inhibition of glycogen synthase kinase 3β. (wiley.com)

As in somatic cells, sperm intracellular cAMP concentration is regulated by the opposite action of two enzymes: the adenylyl cyclases (AC), which synthesize cAMP from ATP, and the phosphodiesterases (PDE), which break the phosphodiester bond of cAMP to form 5'-adenosine monophosphate (AMP). (plos.org)
Adenosine triphosphatase activity of Tritrichomonas foetus. (docksci.com)
revised 25 April 1979) Homogenates of Tritrichomonas foetus exhibited a Mg2+-dependent adenosine triphosphatase (ATPase) activity, with a pH optimum in Tris buffers of 8-2 to 8.3. (docksci.com)

Triazoles are a class of synthetic antifungal and antiprotozoal drugs that inhibit the enzyme lanosterol 14α-demethylase, disrupting ergosterol synthesis in fungal cells. (lookformedical.com)
Using the endothelin-1 (ET-1)-stimulated elevation in cGMP in LLC-PK1 cells as a biological detector system for the conversion of big ET-1 (bET-1) to ET-1, we detected bET-1-converting activities in subcellular fractions from bovine aortic cultured endothelial cells (BAE) and rat brain. (aspetjournals.org)
These results show the presence of at least two different endothelin-converting enzyme activities in endothelial cells and a third within the rat brain. (aspetjournals.org)
This achievement, to fractionate cells on the basis of differential centrifugation, became the first essential technique for cell biologists, since it allowed one to test where a particular activity (like that of an enzyme or a virus) was roughly located in the cell. (rockefeller.edu)
He found that, even in the presence of ample oxygen, cancer cells prefer to metabolize glucose by glycolysis, a seeming paradox as glycolysis, when compared to oxidative phosphorylation, is a less efficient pathway for producing ATP (Warburg, 1956). (pharmaceuticalintelligence.com)

The specific activity of K-pNPPase and its sensitivity to ouabain was significantly increased in the presence of saponin, indicating that part of this activity is latent when assayed on native membrane preparation. (mcmaster.ca)

2 1O6g-min), ~ and a final supernatant (S). In one experiment the large and the small particle fractions were subfractionated by isopycnic centrifugation in a sucrose gradient using the Beaufay rotor (Leighton et al. (docksci.com)

On the other hand, calmodulin and Ca antagonists had no effect on K-pNPPase activity nor its sensitivity to calcium. (mcmaster.ca)

Inhibitor feeding resulted in lesser elastolytic activity compared to control group indicating that extracellular matrix was less damaged. (uni-greifswald.de)

The plasma membrane-enriched fraction isolated from smooth muscle of rat gastric fundus was found to contain a substantial level of potassium-stimulated p-nitrophenylphosphatase activity (K-pNPPase), and its subcellular distribution closely resembled that of other plasma membrane enzyme markers. (mcmaster.ca)

In the initial stages of pancreatitis up to 24 h, the severity markers were found to be significantly lower in the inhibitor treated group. (uni-greifswald.de)

Although Piezo1 channels diffuse readily in the plasma membrane and are widely distributed across the cell, their flicker activity is enriched near force-producing adhesions. (nature.com)
Within the rat brain, phosphoramidon-inhibitable conversion of bET-1 to ET-1 was detected principally in the cytoskeletal fraction, i.e., that fraction from the membrane that was not solubilized by detergent treatment. (aspetjournals.org)

We found that each of the three CK II subunits was located predominantly in the cell nucleus, irrespective of the cell type analyzed or the procedure used for cell fixation. (rupress.org)
During mitosis, the bulk of the enzyme was dispersed throughout the cell, though a fraction of all three subunits was associated with the mitotic spindle. (rupress.org)
The burgeoning stem cell and cancer research activities and the expanding biotechnology and pharmaceutical sectors can be linked to Latin America. (marketdataforecast.com)

Other activities carried out by the IPCS include the development of know-how for coping with chemical accidents, coordination of laboratory testing and epidemiological studies, and promotion of research on the mechanisms of the biological action of chemicals. (inchem.org)
Due to its unique composition, S9 subcellular fractions are highly used in many drugs metabolic studies and pharmacokinetic studies. (kosheeka.com)

Supporting activities include the development of epidemiological, experimental laboratory, and risk-assessment methods that could produce internationally comparable results, and the development of manpower in the field of toxicology. (inchem.org)
A year later Claude found himself in Murphy's laboratory in New York, charged to do just that. (rockefeller.edu)

Find support for a specific problem in the support section of our website. (mdpi.com)

Quercetin is therefore expected to act as a phytochemical by exerting a wide variety of biological activities through these action mechanisms. (rsc.org)
The most comprehensive collection of chemical and biological modi- fications being relevant to mass spectrometry can be found in UniMod [12]. (lu.se)

The Ca2+-inhibition of K-pNNPase was accompanied by increased sensitivity of the enzyme to ouabain. (mcmaster.ca)

The particles' settling velocity in centrifugation is a function of their size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. (wikipedia.org)
Sperm intracellular cAMP levels depend on the activity of adenylyl cyclases, mostly SACY, though transmembrane-containing adenylyl cyclases are also present, and on the activity of cyclic nucleotide phosphodiesterases (PDE) whose role is to degrade cAMP into 5'-AMP. (plos.org)
However, we found that these two subtypes are not always present in this manner, but are rather distributed in a complementary frequency gradient along the posterior midgut. (bioone.org)

Partially purified GST was found to catalyze the conjugation reactions in a fashion similar to that of the isolated subcellular fractions. (aspetjournals.org)

This latter effect may mediate increased Na/H antiporter activity in a number of chronic conditions. (jci.org)

After returning to Poland, Andrzej started his research on the structure-activity relationships of opioid peptides at the Department of Chemistry of Warsaw University. (inrcworld.org)

Anatomy51

Organisms12

Diseases1

Chemicals and Drugs109

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes39

Disciplines and Occupations5

Technology, Industry, Agriculture2

Information Science3

Health Care2

Observed in the cytosolic fraction1

Cytosolic fraction2

Mitochondria7

Metabolism7

Protein12

Fractionation4

Immunosorbent assay1

Phase-II enzymes3

Particulate fraction3

Proteins4

Soluble4

Hepatic2

Regulatory enzyme2

Localization1

Maize2

Cytochrome2

Assays2

Reaction4

Vitro1

Rats4

Liver4

Microsomes2

Metabolite1

Substrate5

Particle fraction1

Catalytic2

Tissue2

Organisms2

Mitochondrial2

Abstract1

Nuclear1

Adult1

Evidence was obtained2

Gene expression2

Characterization1

Cellular2

Tissues1

Signaling pathway1

Medium-sized1

19771

Species2

Metabolic Activity2

Serum1

Activation3

Glucose uptake1

Kinase2

Adenosine3

Cells5

Preparation1

Sucrose1

Calcium1

Extracellular1

Distribution1

Markers1

Membrane2

Cell3

Studies2

Laboratory2

Specific1

Biological2

Inhibition1

Present3

Reactions1

Mediate1

Research1