Enzymes viral tail proteins. Medical search. Frequent questions

Temperate phages are capable of protecting their host from phage reinfection and may change the bacterial phenotype through the expression of viral genes, a process known as lysogenic conversion ( 1 ). (asm.org)
naturally circulating viruses lacked the important contributors to viral pathogenesis: genes encoding proteins functioning in inhibition of cellular transcription and downregulation of the cellular antiviral response. (bio-biz-navi.com)
Soybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic overexpression of proteins-of-interest, because soybean transformation is time-consuming and technically challenging. (researchsquare.com)
First, PCR-amplified marker genes are limited to a narrow subset of the viral community, and require degeneracies and amplification conditions that undermine the quantitative nature of the data ( Sullivan, 2015 ). (peerj.com)
1) Viral genomes vary greatly in size and may include from four genes to several hundred genes. (easynotecards.com)
A) After infection, the viral genes immediately turn the host cell into a lambda-producing factory, and the host cell then lyses. (easynotecards.com)
The study, by a team of 70 researchers led by David S. Hibbett of Clark University, in Worcester, Mass., and Igor V. Grigoriev of the Department of Energy's Joint Genome Institute , took a systematic look at fungal genes that code for lignin-busting enzymes. (acs.org)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

M13 phage displaying an in vivo biotinylatable peptide (AviTag) genetically fused to the phage tail protein pIII were used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (HRP) reporter enzymes covalently attached to the pVIII coat protein. (nih.gov)
We discuss conventional approaches as well as novel strategies, including the use of phage-antibiotic combinations, phage-derived enzymes, exploitation of phage resistance mechanisms, and phage bioengineering. (asm.org)
Is packaged in the phage head and becomes a viral envelope most striking feature is the transfer genetic. (tedxhongkong.org)
Adsorption: The phage attaches to target cell adhesion receptors through its tail fibers. (expasy.org)

In essence, this is the packaging of bacterial DNA into a viral envelope. (tedxhongkong.org)
This T4 head gets a tail and becomes a viral particle and is capable of infecting another bacterial cell. (tedxhongkong.org)
New host cells infected by these viral particles received bacterial DNA instead of viral DNA. (tedxhongkong.org)
Generalized transduction is the process by which any bacterial gene may be transferred to another bacterium via a bacteriophage, and typically carries only bacterial DNA and no viral DNA. (tedxhongkong.org)
Viral particles received bacterial DNA remains attached to the site of prophage are. (tedxhongkong.org)
E. Coli RecA protein the donor bacterium bacterial cell in transfomation is probaly dead bacterium to another by virus. (tedxhongkong.org)
A) Many bacterial cells containing viral DNA are produced. (easynotecards.com)
They observed that when viruses entered the bacteria, the viral DNA was cut into small pieces and inactivated by certain bacterial enzyme. (faqs.org)
Those immune defects lead to infections with bacterial, viral, and fungal pathogens that begin during infancy and, if untreated, result in a fatal outcome in the first few years of life. (medscape.com)
Mice homozygous for disruptions in this gene display immune system abnormalities involving development of both T and B cells and affecting susceptibility to both bacterial and viral infections as well as impaired thermogenic gene expression and energy expenditure. (jax.org)

The Gag-Pol polyprotein contains (within its Pol component) the enzymes viral protease, reverse transcriptase, and integrase. (lifeboat.com)
In contrast to the other two viral targets of HAART, the reverse transcriptase (RT) and protease (PR) enzymes, both of which have singular roles in viral replication, IN contributes a plurality of functions to the viral life cycle, thus potentiating the development for further therapeutic intervention. (rockefeller.edu)

Giampetruzzi and her colleagues used deep sequencing to find similarities to other viruses, and found stretches of viral RNA genome that corresponded to sequences found in the RNA genomes of both Grapevine Berry Inner Necrosis Virus (GINV) and Grapevine Rupestris Stem Pitting-associated Virus (GRSPaV). (wikipedia.org)
Smaller percentages of other viral genomes were also found. (wikipedia.org)
The sequence then proceeds with an untranslated region of 82 base pairs and terminates in the polyA tail that is typical of viral genomes. (wikipedia.org)
The rational design of the genomes was complemented by selection methods which adapted viruses to replication in cells culture and produced variants which (i) shown different levels of replication and production of the individual structural proteins (ii) efficiently induced the antiviral response in infected cells (iii) were incapable of replication in cells of mosquito source and (iv) efficiently replicated in Vero cells. (bio-biz-navi.com)
The genome encodes only 4 nonstructural (ns) proteins nsP1 to -4 which are translated directly from the genomic RNA and interact with host factors to form replicative enzyme complexes (8 34 35 These complexes synthesize the negative-strand RNA intermediates fresh viral genomes and the subgenomic RNA. (bio-biz-navi.com)
Marine viruses impact global biogeochemical cycles via their influence on host community structure and function, yet our understanding of viral ecology is constrained by limitations in host culturing and a lack of reference genomes and 'universal' gene markers to facilitate community surveys. (peerj.com)
The 'VirION' (Viral, long-read metagenomics via MinION sequencing) approach was first validated using mock communities where it was found to be as relatively quantitative as short-read methods and provided significant improvements in recovery of viral genomes. (peerj.com)
E) Viral genomes are usually similar to the genome of the host cell. (easynotecards.com)

Recent updates are briefly described in the status of mRNA vaccines against SARS-CoV-2, influenza virus, and other viral pathogens. (mdpi.com)
The expression of B7 proteins on an antigen-presenting cell is induced by pathogens during the innate response to an infection. (nih.gov)

The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is a candidate vaccine antigen that binds angiotensin-converting enzyme 2(ACE2), leading to virus entry. (cdc.gov)
Upon binding to the ACE2 receptor, the S protein undergoes a conformational change that exposes the RBD and activates the S2 subunit for membrane fusion. (stemcelldaily.com)
Binding of SARS-CoV-2 to the angiotensin‐converting enzyme 2 (ACE2) is a vital pathway for the virus entry into human cells, including those of the respiratory mucosa, esophageal epithelium as well as the absorptive enterocytes from ileum and colon. (irjournal.org)
SARS-CoV-2 uses the metallopeptidase angiotensin-converting enzyme 2 (ACE2) as a viral receptor to enter the human cells. (irjournal.org)

The mRNA acquires a 5' prime cap and 3' prime poly-A tail for protection and translation promotion. (khanacademy.org)
The viral genome, using the machinery of the CEL, produces large amounts d mRNA to synthesize proteins. (wikiteka.com)
The other universal modification found only in eukaryotic mRNA is a poly-A tail. (sciencing.com)
The 5' end of the mRNA is where the RNA polymerase started, and the 3' tail is where it ends. (sciencing.com)
This tail appears to make the mRNA more stable and marks it as destined for export from the nucleus. (sciencing.com)
Some of them like polioviruses and picornaviruses carry an enzyme that chops up a protein required to translate the instructions stored in a 5'-capped mRNA. (sciencing.com)

The Gag polyprotein contains the HIV structural proteins. (lifeboat.com)
It consists of synthesis of specific RNAs, polypeptides, structural proteins, proteinaceous biochemicals or enzymes which control the structure or functioning of specific traits. (qforquestions.in)
Introduction to genomic & Proteomics, Structural genomics and proteomics, Human Genome project and other sequencing projects, Biological databases and data mining, sequence similarity search and sequence alignment, Protein structure predication and structure analysis, use of software package in Bioinformatics. (bits-pilani.ac.in)
To make these viruses incapable of transmission by mosquito vectors and to differentially regulate manifestation of viral structural proteins their replication was made dependent on the internal ribosome access sites derived from additional positive-polarity RNA (RNA+) viruses. (bio-biz-navi.com)
The high rates of alphavirus replication and viremia development are critically determined by efficient function of viral MAP2K2 genome replication machinery and rapid build up of virus-specific structural proteins in the infected cells. (bio-biz-navi.com)
The 26S subgenomic RNA serves as a template for translation of the structural proteins: capsid and glycoproteins E2 and E1 which ultimately form infectious viral particles (43). (bio-biz-navi.com)
Instead they utilize the same structural and nonstructural proteins to function not only in computer virus replication but also in interference with the cellular antiviral response (24-26). (bio-biz-navi.com)
The analysis was based on the presence or absence of antibody response against non-structural protein 1 (NS1) from H1N1pdm09 virus, which was not a component of Pandemrix vaccine. (plos.org)
Non-structural (NS) 1 proteins from recombinant influenza A/Udorn/72 (H3N2) and influenza A/Finland/554/09 (H1N1pdm09) viruses were purified and used in Western blot analysis to determine specific antibody responses in human sera. (plos.org)
The protein HMGB1 behaves as a redox sensor and its structural changes, which are conditioned by the oxidative environment, are associated with different functions of the protein. (hindawi.com)
They have four structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) . The S protein is responsible for binding to the host cell receptor and mediating viral entry. (stemcelldaily.com)
Besides the S protein, SARS-CoV-2 also has three other structural proteins: envelope (E), membrane (M), and nucleocapsid (N). The E protein is the smallest structural protein, with only 75 amino acids. (stemcelldaily.com)

A new study by researchers at Mahidol University in Bangkok, Thailand, reports a COVID-19 vaccine candidate called HexaPro in producing neutralizing antibodies against the SARS-CoV-2 spike protein in an animal model. (news-medical.net)
Numerous species, including rodents, have been found to have antibodies reactive with capsid protein of human HEV strains, and HEV closely related to genotypes 3 or 4 has been recently isolated from rabbits ( 8 ), cattle ( 9 ), and sheep ( 10 ). (cdc.gov)
In ferrets, immunization induced neutralizing antibodies that reduced the upper respiratory viral load upon challenge with a more modern, heterologous H3N2 viral strain. (cdc.gov)
To demonstrate the flexibility and modular nature of the liposome system, 10 recombinant surface antigens representing distinct influenza virus strains were bound simultaneously to generate a highly multivalent protein particle that with 5 ng individual antigen dosing induced antibodies in mice that specifically recognized the constituent immunogens and conferred protection against heterologous H5N1 influenza virus challenge. (cdc.gov)
Instead, paired serum samples from patients, who suffered from a laboratory confirmed H1N1pdm09 infection, showed high levels or diagnostic rises (96%) in H1N1pdm virus NS1-specific antibodies and very high cross-reactivity to H3N2 subtype influenza A virus NS1 protein. (plos.org)

The 5' terminus genome encodes nonstructural proteins (nsps) that are involved in the process of the virus infection cycle. (medrxiv.org)
When admixed with the particle-forming liposomes, immunization reduced viral infection severity at vaccine doses as low as 2 ng HA, highlighting dose-sparing potential. (cdc.gov)
Based on quantitative Western blot analysis, only two of the 45 (4.4%) Pandemrix-vaccinated narcoleptic patients showed specific antibody response against the NS1 protein from the H1N1pdm09 virus, indicating past infection with the H1N1pdm09 virus. (plos.org)
Whether your research is focused on slowing the infection via targeting viral replication or combat the virus by gaining insights on potential repurposed treatments-we have all the tools needed for your cutting edge COVID-19 research. (chromnet.net)
We will also discuss the role of the spike glycoprotein in viral infection and its implications for vaccine development and treatment. (stemcelldaily.com)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

In contrast to some other viruses alphaviruses do not express proteins which are extraneous for RNA replication and virion formation. (bio-biz-navi.com)
Combination antiviral therapy has proven to be particularly effective at suppressing viral replication, yet complete eradication of the virus from an infected individual remains elusive. (rockefeller.edu)
Phosphatidylinositol 3-kinase type 2α (PI3KC2α) is an essential member of the structurally unresolved class II PI3K family with crucial functions in lipid signaling, endocytosis, angiogenesis, viral replication, platelet formation and a role in mitosis. (nature.com)

1 There is a m 7 G-cap (specifically the standard eukaryotic m 7 GpppG as added by the host's enzymes) at the 5' end of the genome and a poly-A tail at the 3' end of the genome. (lifeboat.com)
5' caps and poly-A tails are found in all eukaryotic mRNAs. (sciencing.com)
When a virus infects a eukaryotic cell, the pathogen needs to ensure that the host cell stops producing its own proteins and starts making viral proteins and RNAs instead. (sciencing.com)

Recently, a new class of antiviral drugs targeting the viral integrase (IN) has been added to the HAART (high active antiretroviral therapy) regimen. (rockefeller.edu)

protein particles without any nucleic ác d d causing lethal diseases in mammals, called spongiform encephalopathies pk produce cavities in the brain. (wikiteka.com)
Changes in the redox state of cells affect proteins, lipids, and nucleic acids in different ways. (hindawi.com)

Furthermore, based on sequence analysis it is proposed that Hjc_15-6 has a three-part catalytic motif corresponding to E-SD-EVK, and this motif may be common among other Hj-resolving enzymes originating from thermophilic bacteriophages. (iucr.org)
The DNA-packaging motor in tailed bacteriophages requires nuclease activity to ensure that the genome is packaged correctly. (rcsb.org)
Typically, bacteriophages consist of an outer protein hull enclosing genetic material . (wikidoc.org)

Coronavirus fusion with the cell membrane and internalization also depends on the interaction with the cellular serine protease transmembrane protease serine 2 (TMPRSS2), the cysteine proteases cathepsin B and L, furin and furin-like proteins [ 3 ]. (irjournal.org)

He considered the agent either 1) a stage in the life cycle of the bacteria, 2) an enzyme produced by the bacteria themselves or 3) a virus that grew on and destroyed the bacteria. (wikidoc.org)

This process depends on the general physico-chemical features of the cargo membrane protein and on the interactions of these features with the collective properties of the bilayer, instead of the one-to-one intermolecular interactions that exist between discrete signals and their receptors. (biologists.com)
Recall that an adhesin is a protein or glycoprotein found on the surface of a pathogen that attaches to receptors on the host cell. (pressbooks.pub)

The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. (bio-techne.com)
Signal 1 is provided by a foreign peptide bound to an MHC protein on the surface of the presenting cell. (nih.gov)
This peptide-MHC complex signals through the T cell receptor and its associated proteins. (nih.gov)
It is associated with a complex of invariant transmembrane proteins called CD3 , which transduces the binding of the peptide- MHC complex into intracellular signals ( Figure 24-63 ). (nih.gov)
The S2 subunit contains a fusion peptide, two heptad repeat regions (HR1 and HR2), a transmembrane domain, and a cytoplasmic tail. (stemcelldaily.com)
The fusion peptide inserts into the host cell membrane, while the HR1 and HR2 regions form a six-helix bundle that brings the viral and host membranes closer together, facilitating membrane fusion. (stemcelldaily.com)

Consequently, their cutscreated short single stranded tails on the ends of each fragment, called "sticky ends" or cohesive ends. (faqs.org)
They were known as cohesive ends because they could form complementary base pairs with any other end produced by the same enzyme. (faqs.org)

C) the proteins in the host's cytoplasm. (easynotecards.com)

The findings provide an opening for researchers to use or reengineer fungal enzymes to advance biofuel production. (acs.org)

These modified viral nanoparticles were used in immunochromatographic sandwich assays for the direct detection of IgE and of the penicillin-binding protein from Staphylococcus aureus (PBP2a). (nih.gov)
During the early 1990s, breakthroughs in cloning and sequencing of Norwalk virus and Southampton virus ( 15--18 ) led to the development of sensitive molecular assays (e.g., reverse transcription-polymerase chain reaction [RT-PCR]), nucleotide hybridization probes, and enzyme-linked immunosorbent assays (ELISA) that used baculovirus-expressed viral antigens ( 19--33 ). (cdc.gov)
This was accomplished by conducting a survey of incremental deletions aimed at gradually removing this region of the protein, and testing the resulting viruses in a number of measurable assays of virologic function. (rockefeller.edu)

As such, it represents a new fold for Hj-resolving enzymes from phages. (iucr.org)
The dsDNA tailed phages, or Caudovirales , account for 95% of all the phages reported in the scientific literature, and possibly make up the majority of phages on the planet. (wikidoc.org)

B) Viruses are enclosed in protein capsids rather than plasma membranes. (easynotecards.com)

We then then applied VirION to the first metagenome from a natural viral community from the Western English Channel. (peerj.com)

Tracy says that the poly-A tail helps to promote termination of transcription by the RNA polymerase. (khanacademy.org)
The poly-A tail is not present AT transcription, rather, as transcription finishes, the poly-A tail is immediately added. (khanacademy.org)
To be clear though, the poly-A tail IS NOT present at transcription, and is added quickly to 'lock-in' the termination location. (khanacademy.org)
Following transcription, an enzyme called poly(A) polymerase adds anywhere from 100 to 250 additional adenosine or A subunits, hence the name poly A tail. (sciencing.com)

Together, these findings suggest that VirION provides a high throughput and cost-effective alternative to fosmid and single-virus genomic approaches to more comprehensively explore viral communities in nature. (peerj.com)

Two BARD1 families of normal lipoxygenases can occur AHR depending: directed domain-containing disorders STAT5-mediated as third( download Бюллетень клуба авиастроителей) and IL7 genomic ways( PAH) viral as energy. (familie-vos.de)

Hjc_15-6 was heterologously produced in Escherichia coli and high yields of soluble and biologically active recombinant enzyme were obtained in both complex and defined media. (iucr.org)

However, a zoonosis is a disease that is transmitted from other vertebrates to humans, at least sporadically, without requiring viral mutation. (easynotecards.com)
Most of the therapeutic agents in this review are directed against non-mutable targets of the host, which is independent of viral mutation. (biomedcentral.com)

Angiotensin converting enzyme 2 (ACE-2), also known as ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family. (bio-techne.com)

For membrane proteins, a third mechanism, based on the interaction of their transmembrane domain (TMD) with lipid microdomains, must also be considered. (biologists.com)
Phosphoinositide 3-kinases (PI3Ks) are a family of lipid-modifying enzymes that phosphorylate the 3′-OH group of inositol phospholipids and play key roles in physiology ranging from cell growth and metabolism to organismal development. (nature.com)

Short-read viral metagenomic studies have provided clues to viral function and first estimates of global viral gene abundance and distribution, but their assemblies are confounded by populations with high levels of strain evenness and nucleotide diversity (microdiversity), limiting assembly of some of the most abundant viruses on Earth. (peerj.com)

Peinado H, Del Carmen Iglesias-de la Cruz M, Olmeda D, Csiszar K, Fong KS, Vega S, Nieto MA, Cano A, Portillo F: A molecular role for lysyl oxidase-like 2 enzyme in snail regulation and tumor progression. (bcl-2inhibitors.com)

Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity. (embl.de)

We propose a therapeutic regimen to supplement the current supportive therapy aiming to reduce viral load, the most important factor in the determination of mortality. (biomedcentral.com)

I thought that the addition of the poly-A tail was done as a post-transcriptional modification via polyadenylate polymerase. (khanacademy.org)
The RNA polymerase enzyme travels along a strand of DNA making an RNA copy or transcript. (sciencing.com)

This study describes the production, characterization and structure determination of a novel Holliday junction-resolving enzyme. (iucr.org)

This potentially also explains the increased development of autoantibodies in older people and the association of viral and autoimmune diseases. (medscape.com)
Gastroenteritis Branch in the Division of Viral Diseases at the Centers for Disease Control and Prevention. (cdc.gov)

Ejection of the viral DNA into host cell cytoplasm by long flexible tail ejection system . (expasy.org)
The rest are d microorg metabolic processes necessary for survival within the same cell d but do not need enzymes cél d the host. (wikiteka.com)
viral multiplication whereby d quickly, given the new d training virions released into the environment by lysis and death cél d the host. (wikiteka.com)
E) the proteins on its surface and that of the host. (easynotecards.com)
B) Viral DNA is incorporated into the host genome. (easynotecards.com)
C) The viral genome replicates without destroying the host. (easynotecards.com)
Binding of the S1 domain of the SARS Coronavirus Spike protein to ACE-2 initiates viral entry into the host cell. (bio-techne.com)

The findings suggest the spike protein turns into a locked state to stabilize the spike protein during virus egress. (news-medical.net)

Secretory proteins exit the endoplasmic reticulum (ER) in coat protein complex II (COPII)-coated vesicles and then progress through the Golgi complex before delivery to their final destination. (biologists.com)

D) the enzymes produced by the virus before it infects the cell. (easynotecards.com)
The E and M proteins form the viral envelope and are involved in virus assembly and release. (stemcelldaily.com)
In accordance, viral RNA or live infectious virus have been detected in feces of patients with COVID-19. (irjournal.org)
Furthermore, viral RNA has been detected in COVID-19 patients stool samples [ 8 ] and rectal swabs [ 9 ] even when the virus is no longer found in the upper respiratory tract. (irjournal.org)

In this Commentary, I review evidence in favor of the idea that partitioning of TMDs into bilayer domains that are endowed with distinct physico-chemical properties plays a pivotal role in the transport of membrane proteins within the early secretory pathway. (biologists.com)

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. (embl.de)

To our knowledge, this is the first crystal structure of an Hj-resolving enzyme originating from a bacteriophage that can be classified as an archaeal type of Hj-resolving enzyme. (iucr.org)

SARS-CoV-2 has a genome of 29,903 nucleotides, with 5'-cap structure and 3' poly(A) tail 9 , 10 . (medrxiv.org)

The S protein is composed of two subunits: S1 and S2. (stemcelldaily.com)

Viral tail fiber assembly and viral tail assembly . (expasy.org)

In one subject, just four IgG lineages accounted for 93.5% of the response, including an N-terminal domain (NTD)-directed antibody that was protective against lethal viral challenge. (cdc.gov)

The proteins arethe "work-horses" of the cells and are responsible for carrying out all the functions of the cell. (faqs.org)
If the recipes have extra bases or misspelled bases or if some are deleted, the cell can make a wrong protein or too much or too little of the right one. (faqs.org)
Pure T-cell deficiencies are caused by defects in either a CD3 subunit (such as CD3 δ, CD3 ζ, CD3 ε ) or in CD45 tyrosine phosphatase, key proteins involved in pre-TCR and/or TCR signaling at the positive selection stage. (medscape.com)
HMGB1 is an abundant protein, 10 6 molecules per cell [ 7 ], which has been postulated as a redox sensor [ 8 ]. (hindawi.com)
Signal 2 is provided by costimulatory proteins, especially the B7 proteins (CD80 and CD86), which are recognized by the co-receptor protein CD28 on the surface of the T cell. (nih.gov)
Effector T cells act back to promote the expression of B7 proteins on antigen-presenting cells, creating a positive feedback loop that amplifies the T cell response. (nih.gov)
Scientists already have an arsenal of enzymes to attack cellulose and hemicellulose-the primary components of plant cell walls-to get the component sugars that can be fermented into biofuels. (acs.org)
Recall that fimbriae are hairlike protein bristles on the cell surface. (pressbooks.pub)

Anatomy3

Organisms6

Chemicals and Drugs3

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes5

Information Science3

Genes8

Phage4

Bacterial10

Reverse transcriptase2

Genomes8

Pathogens2

ACE24

MRNA6

Structural12

Antibodies5

Infection6

Replication3

Eukaryotic3

Integrase1

Nucleic2

Bacteriophages3

Protease1

Bacteria1

Receptors2

Peptide6

Cohesive ends2

Host's1

Fungal1

Assays3

Phages2

Membranes1

Natural viral1

Transcription4

Virion1

Genomic1

Recombinant1

Mutation2

Angiotensin1

Lipid2

Viruses1

Tumor1

Ligase1

Mortality1

Polymerase2

Characterization1

Diseases2

Host7

Spike1

Coat protein1

Virus4

Pivotal1

Cellular1

Bacteriophage1

Poly1

Subunits1

Assembly1

Lethal1

Cell8