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PeroxidasesHorseradish PeroxidaseGlutathione PeroxidasePeroxidaseCytochrome-c PeroxidaseAscorbate PeroxidasesEosinophil PeroxidaseIodide PeroxidaseHydrogen PeroxideGuaiacolCatalaseSeleniumLactoperoxidasePeroxiredoxinsLigninOxidation-ReductionSuperoxide DismutaseChloride PeroxidaseBasidiomycotaBenzyl AlcoholsAntioxidantsGlutathione ReductaseOxidative StressHemePeroxidesGlutathioneHistocytochemistryLipid PeroxidationSpectrophotometryKineticsPhanerochaeteBenzidinesCoprinusIodidesSelenoproteinsLipid PeroxidesWheat Germ Agglutinin-Horseradish Peroxidase ConjugatePleurotusSelenious AcidFree RadicalsMalondialdehydeMolecular Sequence DataPeroxiredoxin VICatalysisThyroid Gland3,3'-DiaminobenzidineAmino Acid SequenceHydrogen-Ion ConcentrationElectron Spin Resonance SpectroscopyPeroxiredoxin IIISelenoprotein PIsoenzymesHemeproteinsSelenocysteinePolyporaceaeMicroscopy, ElectronAscorbic AcidPolyporalesReactive Oxygen SpeciesThiobarbituric Acid Reactive SubstancesOxidoreductasesGlucose OxidaseCyanidesEosinophilsEnzymes, ImmobilizedManganeseGlutathione TransferaseAgaricalestert-ButylhydroperoxideArmoraciaThyroglobulinThiocyanatesSelenomethionineOxygenFerrocyanidesSubstrate SpecificityOxidantsLiverPhenolsLaccaseSpectrum Analysis, RamanPinocytosisImmunoenzyme TechniquesGlutathione DisulfideAnisolesSpectrophotometry, UltravioletSodium SeleniteAmitroleErythrocytesBenzene DerivativesThyroiditis, AutoimmunePlantsBromidesVitamin EThioredoxinsTrametesChromatography, High Pressure LiquidOrganoselenium CompoundsRecombinant ProteinsThioredoxin-Disulfide Reductase
SuperoxideHorseradishSubstratesProteinsAntibodiesGlutathione peroxidase activityMyeloperoxidaseOxidoreductaseManganeseAntioxidant enzyme activitiesSubstrateHydrogenOxidoreductasesActivitySeleniumAssayReagentsCoenzymeOxidative stressImmunosorbentEnzymaticTissuesXylanaseExtracellularToxicityAntibodyReactionDeficiencyChromogensImmunoassayEsteraseMicrobialH2O2Thyroid glandDegradationSensitivityFungalBlockingSubstancesBiotinConcentrationSpeciesDetectionActivitiesRadicalsNeutrophilsCatalyzeLiving system
Superoxide5
Horseradish4
  • 1) Horseradish (Armoracia rusticana) peroxidase is used in detection systems with different substrates. (bspp.org.uk)
  • After 3 more washes with PBS/0.5% Tween- cause of the geographically widespread nature of its rodent 20, 100 L of pretitrated staphylococcal protein-A/strepto- host, the deer mouse ( Peromyscus maniculatus ), the most coccal protein-G horseradish peroxidase conjugate (Pierce common mammal in North America. (cdc.gov)
  • In addition, the ImmPRESS Excel Reagent is conjugated to horseradish peroxidase micropolymers, a novel approach that avoids the use of large dextrans or other macromolecules as a backbone. (vectorlabs.com)
  • The team identified mutants of the enzyme, horseradish peroxidase, that catalyze faster. (org.in)
Substrates4
  • In enzymology, a manganese peroxidase (EC 1.11.1.13) is an enzyme that catalyzes the chemical reaction 2 Mn(II) + 2 H+ + H2O2 ⇌ {\displaystyle \rightleftharpoons } 2 Mn(III) + 2 H2O The 3 substrates of this enzyme are Mn(II), H+, and H2O2, whereas its two products are Mn(III) and H2O. (wikipedia.org)
  • Vector Laboratories offers two lines of peroxidase substrates. (2bscientific.com)
  • These heat stable substrates are very useful in multiple labelling experiments and for tissues where blocking endogenous peroxidase is difficult or damages tissues. (2bscientific.com)
  • Assay of glutathione peroxidase in the heart after 6 wk of selenium depletion with both hydrogen peroxide and cumene hydroperoxide as substrates revealed a >80% drop in enzyme activity and gave no indication that murine cardiac tissue contains nonselenium-dependent glutathione peroxidase. (jci.org)
Proteins3
  • Can someone guide me on the references of spectrophotometer methods used to measure enzymes or proteins newly discovered in the blood serum? (researchgate.net)
  • What are the main references (book or research) for one or more, that specify methods for measuring newly discovered enzymes or proteins, in which simple manual methods are used. (researchgate.net)
  • If these enzymes leak out into the bloodstream (as when thyroid cells are damaged) the immune system makes antibodies against these proteins. (medlineplus.gov)
Antibodies6
Glutathione peroxidase activity1
  • In addition, glutathione peroxidase activity was significantly elevated in Epo-treated animals compared to saline-treated animals and the control group. (deu.edu.tr)
Myeloperoxidase3
  • Myeloperoxidase - an enzyme naturally found in our lungs - can biodegrade pristine graphene, according to the latest discovery of Graphene Flagship partners in CNRS, University of Strasbourg (France), Karolinska Institute (Sweden) and University of Castilla-La Mancha (Spain). (spacedaily.com)
  • To test how graphene behaves within the body, researchers analysed how it was broken down with the addition of a common human enzyme - myeloperoxidase or MPO. (spacedaily.com)
  • Myeloperoxidase (MPO) is a human enzyme in the azurophilic granules of neutrophils and in the lysosomes of monocytes. (medscape.com)
Oxidoreductase1
  • The systematic name of this enzyme class is Mn(II):hydrogen-peroxide oxidoreductase. (wikipedia.org)
Manganese1
  • Manganese peroxidase (commonly referred to as MnP) was discovered in 1985 simultaneously by the research groups of Michael H. Gold and Ronald Crawford in the fungus Phanerochaete chrysosporium. (wikipedia.org)
Antioxidant enzyme activities4
  • Brain lipid peroxidation levels and antioxidant enzyme activities were measured in the injured hemispheres 24 h after the hypoxic-ischemic insult. (deu.edu.tr)
  • Increased antioxidant enzyme activities (SOD, APX, and CAT) and upregulation of ROS pathway genes (CAT, APX, GR, and DHAR) were observed in PGPR inoculated okra plants under salinity stress. (hindawi.com)
  • ACC deaminase-containing PGPR can reduce the deleterious effects of environmental stress and can enhance stress tolerance of plants by a variety of mechanisms such as the synthesis of phytohormones, mineral solubilization, nutrient uptake, increased leaf area, increased chlorophyll and soluble protein content, and antioxidant enzyme activities [ 20 ]. (hindawi.com)
  • Less ROS accumulation, upregulation of ROS signaling genes and higher antioxidant enzyme activities post BNS resulted in resistance. (nature.com)
Substrate10
  • The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. (ipl.org)
  • Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate. (ipl.org)
  • 1 "substrate" and another " enzyme. (ipl.org)
  • The three things that can cause the enzyme to denature is a large change in pH level, High Temperature, and substrate concentration. (ipl.org)
  • When we mixed pH 3 enzyme tube with substrate tube, we used 0.3 mL of hydrogen peroxide, but if we were to increase the amount, then the experiment would have been faster. (ipl.org)
  • They do this by forming an enzyme-substrate complex that reduces energy that is required for a specific reaction to occur. (ipl.org)
  • The enzymeʼs have an active site that allows only certain substances to bind, they do this by having an enzyme and substrate that fit together perfectly. (ipl.org)
  • A different reporting enzyme and substrate could help clean up. (2bscientific.com)
  • The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT ® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. (vectorlabs.com)
  • These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. (vectorlabs.com)
Hydrogen4
Oxidoreductases1
Activity15
  • LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. (ipl.org)
  • Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. (ipl.org)
  • To further examine the role of these enzymes, age-related differences in their activity were exploited. (nih.gov)
  • This enzyme needs Ca2+ for activity. (wikipedia.org)
  • Conclusions: These results suggest that Epo exerts neuroprotective effects against hypoxic-ischemic brain injury at least partially via the modulation of antioxidant enzyme activity. (deu.edu.tr)
  • In this study, we characterized plant growth-promoting rhizobacteria (PGPR) containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase and examined their effect on salinity stress tolerance in okra through the induction of ROS-scavenging enzyme activity. (hindawi.com)
  • 2,3,7,8-Tetrachlorodibenzo- p -dioxin as an antiestrogen: Effect on rat uterine peroxidase activity. (cdc.gov)
  • Comparative analysis of the distribution of specific enzymatic activities in tissues of resistant and susceptible varieties before and after infection revealed that the primary localization of activity (except chitinase and peroxidase) was in roots of healthy plants. (usda.gov)
  • Specifically, the activity of all of the studied enzymes except amylase was higher in the resistant variety. (usda.gov)
  • The changes in reduced glutathione (GSH), activity of glutathione transferase (GST) and glutathione peroxidase (GSPx) after administration of exogenous melatonin (N-acetyl-5-methoxy-tryptamine) at a dose 20 mg/kg b.w. were investigated for five and ten days in the liver and kidney of male and female mice. (nih.gov)
  • Glutathione level and activity of studied glutathione enzymes were determined in the supernatants. (nih.gov)
  • Melatonin caused an increase in glutathione level, the activity of glutathione peroxidase as well as glutathione transferase in the examined organs. (nih.gov)
  • Melatonin may exert some effects on the activity of enzymes engaged in the metabolism of thiol compounds in the cell. (nih.gov)
  • Saliva contains a number of components, including various electrolytes, glycoproteins, enzymes, and mucus, as well as secretory immunoglobulin A, lactoferrin, lysozyme, and peroxidase-with the latter 4 components important in that they exhibit antibacterial activity. (medscape.com)
  • Their distinct biosynthetic activity includes an endoperoxidase synthase reaction that oxygenates and cyclizes polyunsaturated fatty acid precursors (eg, arachidonic acid) to form prostaglandin G 2 (PGG2), and a peroxidase reaction that converts PGG2 to prostaglandin H 2 (PGH2), as shown below. (medscape.com)
Selenium1
  • The selenium-deficient state, which was characterized by markedly decreased cardiac glutathione peroxidase levels, led to significantly enhanced doxorubicin toxicity at a dose of 15 mg/kg i.p. (jci.org)
Assay4
  • This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. (vectorlabs.com)
  • Depending on the assay used, one must ensure that eosinophilic peroxidase (EPO) from eosinophils does not cause false-positive results. (medscape.com)
  • females collected were tested for the presence of two phenotypes of P. vivax (PVK210 and PVK247) antigen by enzyme-linked immunosorbent assay (ELISA). (who.int)
  • Total cholesterol, HDL-cholesterol and triglyceride concentrations were measured in serum by a Roch/Hitachi Modular P Chemistry Analyzer (Mod P). The cholesterol assay used the cholesterol oxidase/peroxidase (CHOD/POD) method. (who.int)
Reagents1
  • Of these, fluorescent, biotin, and enzyme labeling reagents are used most frequently for antibody labeling. (dojindo.com)
Coenzyme1
  • Caffeoyl Coenzyme A 3-O-Methyltransferase (CCoAOMT) and Caffeic acid-O-Methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits. (researchgate.net)
Oxidative stress1
Immunosorbent1
  • by using, for the first time in Mo- terrain is very difficult and they are not eas- rocco, an enzyme-linked immunosorbent ily accessible. (who.int)
Enzymatic1
  • The ImmPRESS Excel Amplified HRP (peroxidase) Polymer Staining Kit is an enzymatic, amplification system that produces crisp, highly sensitive, specific staining with low background. (vectorlabs.com)
Tissues2
  • Endogenous Enzymes - Struggling to block enzyme rich tissues? (2bscientific.com)
  • Glutathione peroxidase activities were, however, similar to the two tissues. (jci.org)
Xylanase1
  • Thus, the data suggests that the systemic increase of chitinase, peroxidase, glucanase and xylanase activities may be considered part of cotton's defensive reaction in response to the pathogenic fungus V. dahliae. (usda.gov)
Extracellular1
  • 2005). These fungi produce sever- lected in the present study by enrichment culture, using kraft al extracellular peroxidases, laccases, and additional oxidative lignin and kraft pulping lignin-enriched streams. (lu.se)
Toxicity5
  • The role of the glutathione peroxidase/reductase (GSH-Px/GSSG-Rd) enzyme system in protection from paracetamol toxicity was investigated in isolated mouse hepatocytes in primary culture. (nih.gov)
  • The effect of inhibitors of these enzymes on the toxicity of paracetamol and on t-butylhydroperoxide (t-BOOH), used as a positive control, was determined. (nih.gov)
  • However, they also increased the susceptibility of hepatocytes to paracetamol toxicity, indicating that a component of paracetamol's toxic effect involves formation of species that are detoxified by the GSH-Px/GSSG-Rd enzymes. (nih.gov)
  • The data indicate that the GSH-Px/GSSG-Rd enzymes contribute to protection from paracetamol toxicity and suggest that formation of peroxides contributes to this drug's hepatotoxic effects. (nih.gov)
  • The role of peroxidase and IAA oxidase activities in defense mechanisms in response to heavy metal toxicity is discussed. (tubitak.gov.tr)
Antibody4
  • The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. (vectorlabs.com)
  • These unique micropolymers allow a higher density of enzymes per antibody to bind the target with minimal interference. (vectorlabs.com)
  • Thyroid peroxidase (TPO, antimicrosomal antibody, antithyroid microsomal antibody) antibody - blood. (medlineplus.gov)
  • Biotinylated anti-human IgG labeled with strepavidin-peroxidase is used to detect the patient's antibody. (cdc.gov)
Reaction2
  • About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. (ipl.org)
  • After the peroxidase reaction, wash slide with buffer 5-7X, (this buffer should not contain any sodium azide because peroxidase is inactivated) and distilled/deionized water 2X. (immunochemistry.com)
Deficiency4
  • Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. (curehunter.com)
  • Although MPO received little clinical attention until 1966, the enzyme was first isolated in 1941, and deficiency of MPO was first described in 1954. (medscape.com)
  • a deficiency of this enzyme leads to a loss of the iodotyrosine derivatives and iodine from the thyroid and results in goiter. (theodora.com)
  • Regardless of the etiology, a deficiency of cyclooxygenase (COX), a key regulatory enzyme in the synthetic pathway of eicosanoid production, results in beneficial and detrimental physiologic conditions relative to imbalances of the eicosanoids. (medscape.com)
Chromogens1
Immunoassay1
  • and Prevention (CDC) enzyme immunoassay (EIA) ( 5 ). (cdc.gov)
Esterase1
  • HDL cholesterol was measured by a direct non-precipitating method using polyethylene glycol-coupled cholesteryl esterase (PEG-modified enzyme) and cholesterol oxidase. (who.int)
Microbial2
  • Microbial Enzymes and Their Mechanisms in the Bioremediation of Pollutants. (igi-global.com)
  • Microbial enzymes have a wide range of metabolic activities and are involved in the transformation of pollutants. (igi-global.com)
H2O21
Thyroid gland2
Degradation1
Sensitivity1
  • The ImmPRESS Excel Amplified Staining System capitalizes on all the advantages of the ImmPRESS polymerized reporter enzyme staining system technology but offers additional sensitivity and convenience. (vectorlabs.com)
Fungal8
  • For this purpose, basidiomycetes secrete MnP, rather than Mn(III), and the enzyme functions outside of the fungal cell. (wikipedia.org)
  • In some occupational environments, workers are at an increased risk of exposure to fungal enzymes used in industrial production. (cdc.gov)
  • Fungal enzymes have been associated with adverse health effects in the work place, in particular in baking occupations. (cdc.gov)
  • Exposure-response relationships have been demonstrated, and atopic workers directly handling fungal enzymes are at an increased risk for IgE-mediated disease and occupational asthma. (cdc.gov)
  • The utilization of new and emerging fungal enzymes in industrial production will present new occupational exposures. (cdc.gov)
  • Allergen avoidance strategies including personal protective equipment, engineering controls, protein encapsulation, and reduction of airborne enzyme concentrations are required to mitigate occupational exposure to fungal enzymes. (cdc.gov)
  • 6]. Some of the best examples of high-molecular-weight sectors, workers are at increased risk of becoming sensitized occupational allergens are the fungal enzymes. (cdc.gov)
  • Table 1: Fungal enzymes utilized in different industries and associations with occupational sensitization. (cdc.gov)
Blocking1
Substances1
  • In addition, a number of nutrient substances, vitamins, and minerals have been shown to contribute to a. functions, generally by serving as co-factors or co-enzymes. (theodora.com)
Biotin2
  • Streptavidin Biotin Enzyme Complex Peroxidase Sabc Pod Goat Anti Rabbit Igg Kit, supplied by Boster Bio, used in various techniques. (bioz.com)
  • These kits can be used to prepare conjugates with enzyme, fluorophore, and biotin (Table 1). (dojindo.com)
Concentration1
  • Immunologically mediated OA accounts for approximately weight allergens in industrial settings and describe the 90% of cases [9], but the severity of disease is dependent main enzymes that have been associated with occupational on the concentration, route, agent of exposure, and the sensitization and asthma. (cdc.gov)
Species2
  • The enzyme is thought to be unique to Basidiomycota as no bacterium, yeast, or mold species has yet been found which naturally produces it. (wikipedia.org)
  • As no functional data are available in regards to this enzyme in monocotyledonous species, we generated C3H1 knock-down maize plants. (researchgate.net)
Detection1
  • What are the advantages of using ImmPRESS® Excel kits compared with one-step peroxidase polymer detection systems? (vectorlabs.com)
Activities1
  • Peroxidase and IAA oxidase activities showed a clear inverse relation with growth. (tubitak.gov.tr)
Radicals1
  • Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)
Neutrophils2
  • Found in the human lungs, MPO is a peroxide enzyme released by neutrophils, a type of cells responsible for the elimination of any foreign bodies. (spacedaily.com)
  • The easiest technique is to perform direct visualization of neutrophils on a peripheral blood smear that has been stained for peroxidase. (medscape.com)
Catalyze1
  • Ascorbate peroxidase has vital defensive role against ROS [ 17 ] and can catalyze the breakdown of H 2 O 2 that is produced by SOD. (hindawi.com)
Living system1
  • Enzymes are needed for survival in any living system and they control cellular reactions. (ipl.org)