• The systematic name of this enzyme class is chorismate pyruvate-lyase (4-hydroxybenzoate-forming). (wikipedia.org)
  • It is also called chorismate pyruvate lyase (4-hydroxybenzoate-forming) and it is also abbreviated several different ways: CPL, CL, and ubiC. (wikipedia.org)
  • protein_coding" "Cz10g28290.t1","No alias","Chromochloris zofingiensis","Chorismate pyruvate-lyase Rv2949c-like [Interproscan]. (ntu.edu.sg)
  • more specifically the ox-acid-lyase or the carbon-carbon-lyases. (wikipedia.org)
  • The enzyme chorismate lyase (EC 4.1.3.40) catalyzes the first step in ubiquinone biosynthesis, the removal of pyruvate from chorismate, to yield 4-hydroxybenzoate in Escherichia coli and other Gram-negative bacteria. (wikipedia.org)
  • This enzyme has an optimum pH at 7.5 Inhibited by: Vanillate 4-hydroxybenzaldehyde 3-carboxylmethylaminmethyl-4-hydroxybenzoic acid 4HB - ubiC is inhibited by the product of the reaction, which scientists believe serves as a control mechanism for the pathway The pathway used is called the ubiquinone biosynthesis pathway, it catalyzes the first step in the biosynthesis of ubiquinone in E. coli. (wikipedia.org)
  • This enzyme participates in ubiquinone biosynthesis. (np-mrd.org)
  • This enzyme catalyses the first step in ubiquinone biosynthesis in Escherichia coli and other Gram-negative bacteria. (np-mrd.org)
  • In some organisms this enzyme is part of a multifunctional protein that also includes one or more of the enzymes EC 2.4.2.18 (anthranilate phosphoribosyltransferase), EC 4.1.1.48 (indole-3-glycerol-phosphate synthase), EC 4.1.3.27 (anthranilate synthase) and EC 5.3.1.24 (phosphoribosylanthranilate isomerase). (enzyme-database.org)
  • Woehl, E. and Dunn, M.F. Mechanisms of monovalent cation action in enzyme catalysis: the tryptophan synthase α-, β-, and αβ-reactions. (enzyme-database.org)
  • Within the framework of a DFG research group (For 1296), we investigated the use of the enzyme MenD (SEPHCHC synthase) as a novel biocatalyst for 1,2- and 1,4-additions in cooperation with the group of Jürgen Pleiss (ITB, Univ. (uni-stuttgart.de)
  • This enzyme catalyses the chemical reaction: chorismate ⇌ {\displaystyle \rightleftharpoons } 4-hydroxybenzoate + pyruvate Its activity does not require metal cofactors. (wikipedia.org)
  • Such cases exist: for example, a mutase such as phosphoglucomutase catalyses the transfer of a phospho group from one position to another, and isomerase is a more general term for an enzyme that catalyses any one-substrate one-product reaction, such as triosephosphate isomerase . (wikipedia.org)
  • In enzyme science , 4-hydroxy-2-oxovalerate aldolase is a Lyase Enzyme Reaction , which requires Mn 2+ for maximal activity. (wellnessadvocate.com)
  • An enzyme that catalyzes the formation of anthranilate (o-aminobenzoate) and pyruvic acid from chorismate and glutamine. (nih.gov)
  • Structures of the enzyme with substrate and covalent pre- and post-dehydration reaction intermediates provide snapshots of successive steps along the type I DHQD-catalyzed reaction coordinate. (rcsb.org)
  • In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated. (wikipedia.org)
  • This rate-determining step may be a chemical reaction or a conformational change of the enzyme or substrates, such as those involved in the release of product(s) from the enzyme. (wikipedia.org)
  • in such cases, it is helpful to determine the enzyme structure with and without bound substrate analogues that do not undergo the enzymatic reaction. (wikipedia.org)
  • Beyond this limit the enzyme is saturated with substrate and the reaction rate ceases to increase. (wikipedia.org)
  • The reaction catalysed by an enzyme uses exactly the same reactants and produces exactly the same products as the uncatalysed reaction. (wikipedia.org)
  • In the first step of this pathway, chorismate lyase transforms chorismate into 4-hydroxybenzoate and pyruvate (PMID 1644758). (foodcomex.org)
  • The biosynthetic shikimate pathway consists of seven enzymes that catalyze sequential reactions to generate chorismate, a critical branch point in the synthesis of the aromatic amino acids. (rcsb.org)
  • The fact that the shikimate pathway is absent from humans makes the enzymes of the pathway potential targets for the development of non-toxic antimicrobials. (rcsb.org)
  • Various thiamine diphosphate (ThDP)-dependent enzymes can - apart from their functions in metabolism - also be used as biocatalysts for C-C bond linkages. (uni-stuttgart.de)
  • Studying an enzyme's kinetics in this way can reveal the catalytic mechanism of this enzyme, its role in metabolism , how its activity is controlled, and how a drug or a modifier ( inhibitor or activator ) might affect the rate. (wikipedia.org)
  • An example of enzymes that bind a single substrate and release multiple products are proteases , which cleave one protein substrate into two polypeptide products. (wikipedia.org)
  • Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
  • Malic enzyme, N-terminal domain [Interproscan]. (ntu.edu.sg)
  • The main difference between ribozymes and enzymes is that RNA catalysts are composed of nucleotides, whereas enzymes are composed of amino acids. (wikipedia.org)
  • Chorismic acid, more commonly known as its anionic form chorismate, is an important biochemical intermediate in plants and microorganisms. (ecmdb.ca)
  • citation.page_first 8035 _citation.page_last 8044 _citation.title 'Self-Immolation of a Bacterial Dehydratase Enzyme by its Epoxide Product. (rcsb.org)
  • It belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. (wikipedia.org)
  • We are investigating enzymes that can make or cleave C-C bonds, as these are suitable biocatalysts in chemo-enzymatic syntheses, e.g. to produce novel hydroxylated compounds. (uni-stuttgart.de)
  • Taxonomic lineage: bacteria → proteobacteria → gammaproteobacteria → enterobacteriales → enterobacteriaceae → escherichia → Escherichia coli This enzyme is a monomer. (wikipedia.org)
  • In this scenario acetate synthesized from pyruvate with PoxB was apparently reutilized via Acs and the glyoxylate shunt enzymes. (biomedcentral.com)
  • chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7. (expasy.org)
  • For the first time, the enzyme:substrate complex of the Schiff base type was elucidated for aldolases. (uni-stuttgart.de)
  • Likewise, other chorismate-derived substances such as violacein (via the intermediate L-tryptophan) could be obtained. (uni-stuttgart.de)
  • 4-Hydroxybenzoate is an intermediate in many enzyme-mediated reactions in microbes. (np-mrd.org)
  • Enzyme kinetics is the study of the rates of enzyme-catalysed chemical reactions . (wikipedia.org)
  • This enzyme participates in toluene and xylene degradation in bacteria such as Pseudomonas mendocina. (np-mrd.org)
  • 4-hydroxybenzoate and chloride are produced when the enzyme 4-chlorobenzoate dehalogenase acts on the halide bond of 4-chlorobenzoate with H2O (PMID: 6497878). (foodcomex.org)
  • This enzyme participates in 2,4-dichlorobenzoate degradation and is found in Candida parapsilosis. (np-mrd.org)
  • This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation and is found in Pseudomonas putida and Pseudomonas fluorescens. (np-mrd.org)
  • This enzyme is involved in the degradation of N -acetylneuraminate. (qmul.ac.uk)
  • Furthermore, the transcriptional responses of B5 at different temperature by using rice straw as sole carbon sources were also analyzed, based on which the mechanism of lignocellulose degradation at high temperature was revealed that B5 could resist the heat by up-regulating the heat shock proteins (HSP) and then secrete various Carbohydrate-Active enzymes (CAZymes) to realize energy balance. (researchsquare.com)
  • Moreover, an intermediate-enzyme adduct with a NOS-covalent linkage was observed in the later site, where it acts as a two-scissile-bond linkage facilitating nucleophilic addition and cofactor-free decarboxylation. (hokudai.ac.jp)
  • For instance, the enzyme 4-hydroxybenzaldehyde dehydrogenase uses 4-hydroxybenzaldehyde, NAD+ and H2O to produce 4-hydroxybenzoate, NADH and H+. (np-mrd.org)
  • The structures and mechanistic insight presented here may inform the design of type I DHQD enzyme inhibitors. (rcsb.org)
  • The resultant product is no longer sensitive to aminoglycoside-modifying enzymes, a strategy that antibiotic-producing species employ to counter drug resistance in competing species. (hokudai.ac.jp)
  • The active centres of the enzymes were changed by site-directed mutagenesis and these "muteins" were then investigated for their catalytic properties. (uni-stuttgart.de)
  • That enzyme cannot combine with the α unit of EC 4.2.1.20 to form a complex. (enzyme-database.org)
  • In collaboration with Prof. W.D. Fessner's group at the TU Darmstadt, the directed evolution of transaldolase to obtain new enzyme properties was successfully investigated. (uni-stuttgart.de)
  • Here, we report a noncanonical FAD-dependent enzyme Orf1 that adds a glycine-derived N-formimidoyl group to glycinothricin to form the antibiotic BD-12. (hokudai.ac.jp)