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  • vitro
  • Mixtures of human leukocyte antigen A2 (HLA-A2)-binding ovarian cancer-associated peptides were used to activate naive T cells to generate antigen-specific T cells that could recognize ovarian and breast cancers in vitro . (aacrjournals.org)
  • T cells specific for individual peptides could be generated in vitro by using mixtures of peptides, and these T cells recognized ovarian and breast cancers but not nonmalignant cells. (aacrjournals.org)
  • Mixtures of specific peptides identified as naturally presented on cancer cells and capable of activating tumor-specific T cells in vitro also initiate or augment immune responses toward solid tumors in cancer patients. (aacrjournals.org)
  • The evidence for the role of the microbiota in metabolism of dietary components and for its impact on health is derived from comparative studies in germ-free and conventional microbiota, or human microbiota-associated animals, and from in vitro studies using human faecal incubations or more complex continuous culture gut models. (springer.com)
  • Human proteins expressed by in vitro translation have high isotope incorporation. (thermofisher.com)
  • molecules
  • In electrospray, the ions are created from proteins in solution, and it allows fragile molecules to be ionized intact, sometimes preserving non-covalent interactions. (wikipedia.org)
  • The purpose of this study is to test whether peptide epitopes chosen from among those naturally processed and overpresented within MHC molecules by malignant, but not normal cells, when formulated into cancer vaccines, could activate antitumor T-cell responses in humans. (aacrjournals.org)
  • The central hypothesis in breast and ovarian cancer immunotherapy is that these cancers express antigens in the form of peptides presented by MHC class I molecules that can be recognized by cytolytic T cells (CTL) leading to tumor destruction ( 1, 2 ). (aacrjournals.org)
  • A promising, clinically relevant strategy for prioritizing peptide targets for therapeutic application would be to identify those peptides abundantly presented within the MHC class I molecules by tumor cells. (aacrjournals.org)
  • An unbiased analysis of the peptide repertoire associated with the MHC class I molecules of cancer cells could identify and allow the prioritization of novel tumor antigens for the development of cancer immunotherapy (reviewed in ref. 21 ). (aacrjournals.org)
  • extract
  • Panel A: Western blot of GST-fusion proteins GFP, BAD, cyclinD1, retinoblastoma (RB), p53 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expressed using human IVT extract (the arrow indicates an anti-GST cross-reacting band in the lysate). (thermofisher.com)
  • cellular
  • The extent of GlcNAcylation on nucleocytoplasmic proteins is highly sensitive to the concentrations of glucose and other nutrients surrounding cells and to nearly all types of cellular stress. (diabetesjournals.org)
  • Based on these findings, we hypothesize that changes in the O-GlcNAc levels on some erythrocyte proteins may be used diagnostically to monitor the history of cellular exposure to changes in nutrients, especially glucose, and to oxidative stress. (diabetesjournals.org)
  • clinical
  • Combinations of these peptides (0.3 mg of each peptide or 1 mg of each peptide) were formulated into vaccines in conjunction with Montanide ISA-51 and granulocyte monocyte colony stimulating factor which were used to vaccinate patients with ovarian and breast cancer without evidence of clinical disease in parallel pilot clinical trials. (aacrjournals.org)
  • affinity
  • 6. The method of claim 4, further comprising washing ADC bound to the affinity capture media to reduce non-antibody proteins in contact with the ADC. (patents.com)
  • 8. The method of claim 4, wherein the step of digesting the denatured antibody to form a digested antibody-drug conjugate peptide mixture occurs while the ADC is bound to the affinity capture media. (patents.com)
  • specific
  • Nine of the 14 vaccinated patients responded immunologically to their vaccine by inducing peptide-specific T-cell responses that were capable of recognizing HLA-matched breast and ovarian cancer cells. (aacrjournals.org)
  • These data suggest not only that site-specific O-GlcNAc levels reflect the glycemic status of an individual but also that O-GlcNAc site occupancy on erythrocyte proteins may be eventually useful as a diagnostic tool for the early detection of diabetes. (diabetesjournals.org)
  • microbial
  • The ability to assess the entire protein network of a cell at a given spatial or temporal point will have a profound effect upon microbial science as the function of proteins is inextricably linked to phenotype. (biomedcentral.com)
  • In the first part of the review, we discuss the main gut microorganisms, particularly bacteria, and microbial pathways associated with the metabolism of dietary carbohydrates (to short chain fatty acids and gases), proteins, plant polyphenols, bile acids, and vitamins. (springer.com)
  • method
  • It also discloses to the use of this reagent in a method for digesting hemoglobin, in a method for detecting HbA1c, and to a sampling tube for collection of a whole blood sample comprising said reagent for digesting hemoglobin. (patentsencyclopedia.com)
  • form
  • In MALDI, the proteins are embedded within a matrix normally in a solid form, and ions are created by pulses of laser light. (wikipedia.org)
  • identification
  • Proteins are identified using combined evidence from constituent peptides, resulting in a list in which each protein is associated with a score signifying the confidence of correct identification. (pubmedcentralcanada.ca)
  • As a result, only a percentage of the expected proteins are identified with confidence despite presence in the biological sample, and the MS/MS identification scores of many other proteins fall below acceptable confidence thresholds. (pubmedcentralcanada.ca)