• molecules
  • In electrospray, the ions are created from proteins in solution, and it allows fragile molecules to be ionized intact, sometimes preserving non-covalent interactions. (wikipedia.org)
  • The purpose of this study is to test whether peptide epitopes chosen from among those naturally processed and overpresented within MHC molecules by malignant, but not normal cells, when formulated into cancer vaccines, could activate antitumor T-cell responses in humans. (aacrjournals.org)
  • This study tests the hypothesis that choosing peptides, naturally processed and overpresented within MHC molecules by malignant, but not normal cells, would allow functional prioritization of which peptides to formulate into cancer vaccines capable of activating antitumor T-cell responses in humans. (aacrjournals.org)
  • The central hypothesis in breast and ovarian cancer immunotherapy is that these cancers express antigens in the form of peptides presented by MHC class I molecules that can be recognized by cytolytic T cells (CTL) leading to tumor destruction ( 1, 2 ). (aacrjournals.org)
  • A promising, clinically relevant strategy for prioritizing peptide targets for therapeutic application would be to identify those peptides abundantly presented within the MHC class I molecules by tumor cells. (aacrjournals.org)
  • An unbiased analysis of the peptide repertoire associated with the MHC class I molecules of cancer cells could identify and allow the prioritization of novel tumor antigens for the development of cancer immunotherapy (reviewed in ref. 21 ). (aacrjournals.org)
  • secretion
  • These findings show that secretion and secreted proteins are key elements in the virulence and adaptation of C. pseudotuberculosis . (frontiersin.org)
  • This catalytic activity is shown to be intimately linked with the secretion of the chitinolytic machinery, since substitution of the ChiX Asp-120 residue results in a variant protein that is both unable to digest peptidoglycan and cannot rescue the phenoytype of a chiX mutant strain. (biochemj.org)
  • Inactivation of either chiW or chiX impairs secretion of the entire chitinolytic machinery with the proteins accumulating in the periplasm unable to cross the outer membrane [ 11 ]. (biochemj.org)
  • Interestingly, the chitinase-secretion-defective phenotype of a chiW mutant can be rescued if the signal peptide from another protein is attached to ChiX [ 11 ]. (biochemj.org)
  • clinical
  • Combinations of these peptides (0.3 mg of each peptide or 1 mg of each peptide) were formulated into vaccines in conjunction with Montanide ISA-51 and granulocyte monocyte colony stimulating factor which were used to vaccinate patients with ovarian and breast cancer without evidence of clinical disease in parallel pilot clinical trials. (aacrjournals.org)
  • specific
  • Collision-induced dissociation is used in mainstream applications to generate a set of fragments from a specific peptide ion. (wikipedia.org)
  • T cells specific for individual peptides could be generated in vitro by using mixtures of peptides, and these T cells recognized ovarian and breast cancers but not nonmalignant cells. (aacrjournals.org)
  • Nine of the 14 vaccinated patients responded immunologically to their vaccine by inducing peptide-specific T-cell responses that were capable of recognizing HLA-matched breast and ovarian cancer cells. (aacrjournals.org)
  • Mixtures of specific peptides identified as naturally presented on cancer cells and capable of activating tumor-specific T cells in vitro also initiate or augment immune responses toward solid tumors in cancer patients. (aacrjournals.org)
  • These data suggest not only that site-specific O-GlcNAc levels reflect the glycemic status of an individual but also that O-GlcNAc site occupancy on erythrocyte proteins may be eventually useful as a diagnostic tool for the early detection of diabetes. (diabetesjournals.org)
  • species
  • The Cryptococcus neoformans/C. gattii species complex comprises two sibling species that are divided into eight major molecular types, C. neoformans VNI to VNIV and C. gattii VGI to VGIV. (jove.com)
  • determine
  • While these proteins determine longevity in mammals, they do appear to direct acetylome signaling networks in response to caloric restriction ( 4, 5 ). (aacrjournals.org)
  • given
  • Because of this simplicity in fragmentation, it is possible to use the observed fragment masses to match with a database of predicted masses for one of many given peptide sequences. (wikipedia.org)
  • addition
  • In addition, two N-glycosylation sites are located in the crystallizable fragment (Fc) domain of the mAb occupied with complex and high mannose type glycans, which are known to play key roles in safety and efficacy (5,6). (chromatographyonline.com)
  • highly
  • Advances in instrumentation, column technology, and reagents are providing analysts with a new set of tools to broaden their understanding of the highly complex products they are studying. (chromatographyonline.com)
  • INTRODUCTION
  • While the technique has been used for more than 20 years to profile enzymatically released and fluorescently labelled N-glycans, the introduction of new columns (sub-2-┬Ám and widepore) has paved the way to explore the technique further. (chromatographyonline.com)