• molecules
  • In electrospray, the ions are created from proteins in solution, and it allows fragile molecules to be ionized intact, sometimes preserving non-covalent interactions. (wikipedia.org)
  • The purpose of this study is to test whether peptide epitopes chosen from among those naturally processed and overpresented within MHC molecules by malignant, but not normal cells, when formulated into cancer vaccines, could activate antitumor T-cell responses in humans. (aacrjournals.org)
  • The central hypothesis in breast and ovarian cancer immunotherapy is that these cancers express antigens in the form of peptides presented by MHC class I molecules that can be recognized by cytolytic T cells (CTL) leading to tumor destruction ( 1, 2 ). (aacrjournals.org)
  • A promising, clinically relevant strategy for prioritizing peptide targets for therapeutic application would be to identify those peptides abundantly presented within the MHC class I molecules by tumor cells. (aacrjournals.org)
  • An unbiased analysis of the peptide repertoire associated with the MHC class I molecules of cancer cells could identify and allow the prioritization of novel tumor antigens for the development of cancer immunotherapy (reviewed in ref. 21 ). (aacrjournals.org)
  • extract
  • Panel A: Western blot of GST-fusion proteins GFP, BAD, cyclinD1, retinoblastoma (RB), p53 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expressed using human IVT extract (the arrow indicates an anti-GST cross-reacting band in the lysate). (thermofisher.com)
  • cellular
  • The extent of GlcNAcylation on nucleocytoplasmic proteins is highly sensitive to the concentrations of glucose and other nutrients surrounding cells and to nearly all types of cellular stress. (diabetesjournals.org)
  • Based on these findings, we hypothesize that changes in the O-GlcNAc levels on some erythrocyte proteins may be used diagnostically to monitor the history of cellular exposure to changes in nutrients, especially glucose, and to oxidative stress. (diabetesjournals.org)
  • clinical
  • Combinations of these peptides (0.3 mg of each peptide or 1 mg of each peptide) were formulated into vaccines in conjunction with Montanide ISA-51 and granulocyte monocyte colony stimulating factor which were used to vaccinate patients with ovarian and breast cancer without evidence of clinical disease in parallel pilot clinical trials. (aacrjournals.org)
  • different
  • We identify 8-29% more proteins than the original MS experiment when applied to yeast grown in different experimental conditions analyzed on different MS/MS instruments, and 37% more proteins in a human sample. (pubmedcentralcanada.ca)
  • It can also be used to localize proteins to the various organelles, and determine the interactions between different proteins as well as with membrane lipids. (wikipedia.org)
  • The digest conditions are such that it contains many different peptides including those of higher molecular weight (proteoses). (sigmaaldrich.com)
  • specific
  • Collision-induced dissociation is used in mainstream applications to generate a set of fragments from a specific peptide ion. (wikipedia.org)
  • Nine of the 14 vaccinated patients responded immunologically to their vaccine by inducing peptide-specific T-cell responses that were capable of recognizing HLA-matched breast and ovarian cancer cells. (aacrjournals.org)
  • Here, we investigated the extent of GlcNAcylation on human erythrocyte proteins and compared site-specific GlcNAcylation on erythrocyte proteins from diabetic and normal individuals. (diabetesjournals.org)
  • These data suggest not only that site-specific O-GlcNAc levels reflect the glycemic status of an individual but also that O-GlcNAc site occupancy on erythrocyte proteins may be eventually useful as a diagnostic tool for the early detection of diabetes. (diabetesjournals.org)
  • species
  • The Cryptococcus neoformans/C. gattii species complex comprises two sibling species that are divided into eight major molecular types, C. neoformans VNI to VNIV and C. gattii VGI to VGIV. (jove.com)
  • identification
  • Proteins are identified using combined evidence from constituent peptides, resulting in a list in which each protein is associated with a score signifying the confidence of correct identification. (pubmedcentralcanada.ca)
  • As a result, only a percentage of the expected proteins are identified with confidence despite presence in the biological sample, and the MS/MS identification scores of many other proteins fall below acceptable confidence thresholds. (pubmedcentralcanada.ca)
  • given
  • Because of this simplicity in fragmentation, it is possible to use the observed fragment masses to match with a database of predicted masses for one of many given peptide sequences. (wikipedia.org)
  • addition
  • In addition, two N-glycosylation sites are located in the crystallizable fragment (Fc) domain of the mAb occupied with complex and high mannose type glycans, which are known to play key roles in safety and efficacy (5,6). (chromatographyonline.com)