• Ella is an instrument that allows automated ELISA assays where everything is pre-loaded onto a specific cartridge. (labion.eu)
  • BioVision's rat IL-6 ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. (biovision.com)
  • Current methods for detecting BoNT include a mouse cause human botulism (A, B, E, and F). We developed a bioassay (2-4) and an enzyme-linked immunosorbent assay rapid, mass spectrometry-based method (Endopep-MS) to (ELISA) (5,6). (cdc.gov)
  • From ELISA development and plate coating to conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay. (immunochemistry.com)
  • The Fetuin A ELISA Assay Kit is intended for the quantitative determination of human Fetuin-A, also known as alpha-2-HS glycoprotein (AHSG), in serum, plasma, cell culture supernatant, tissue extraction and urine. (eaglebio.com)
  • The Eagle Biosciences Fetuin A ELISA Assay kit is for research use only and not to be used in diagnostic procedures. (eaglebio.com)
  • This Fetuin A ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human Fetuin-A in serum samples. (eaglebio.com)
  • Our ELISA Kit assays are dynamic research tools and may be updated and improved from time to time. (bioconstructor.com)
  • MBS765028 is a ready-to-use microwell and strip plate ELISA (Enzyme-Linked Immunosorbent Assay) Kit for testing the presence of Immunoglobulin G (IgG) ELISA Kit target analytes in biological samples. (bioconstructor.com)
  • Enzymes can also be applied to assay kits, such as coupled multi-enzyme assay kits and enzyme-linked immunosorbent assay (ELISA) kits, to conveniently conduct tests and diagnosis. (infinitabiotech.com)
  • The substrate- or cofactor-based fluorine NMR screening, also known as n-FABS (n fluorine atoms for biochemical screening), represents a powerful method for performing a direct functional assay in the search of inhibitors or enhancers of an enzymatic reaction. (novartis.com)
  • Researchers at BellBrook Labs have developed a high throughput screening (HTS) compatible biochemical assay to discover and advance small molecule modulators for the target cyclic GMP-AMP synthase (cGAS). (labbulletin.com)
  • BellBrook's core technology Transcreener is a universal, homogenous biochemical assay platform based on detection of nucleotides that makes it easy to screen thousands of different enzymes. (labbulletin.com)
  • Enzymes are biological catalysts (also known as biocatalysts) that speed up biochemical reactions in living organisms, and which can be extracted from cells and then used to catalyse a wide range of commercially important processes. (infinitabiotech.com)
  • Enzyme kinetics is the study of the rates of enzyme-catalysed chemical reactions . (wikipedia.org)
  • In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated. (wikipedia.org)
  • Such cases exist: for example, a mutase such as phosphoglucomutase catalyses the transfer of a phospho group from one position to another, and isomerase is a more general term for an enzyme that catalyses any one-substrate one-product reaction, such as triosephosphate isomerase . (wikipedia.org)
  • This rate-determining step may be a chemical reaction or a conformational change of the enzyme or substrates, such as those involved in the release of product(s) from the enzyme. (wikipedia.org)
  • in such cases, it is helpful to determine the enzyme structure with and without bound substrate analogues that do not undergo the enzymatic reaction. (wikipedia.org)
  • Beyond this limit the enzyme is saturated with substrate and the reaction rate ceases to increase. (wikipedia.org)
  • The reaction catalysed by an enzyme uses exactly the same reactants and produces exactly the same products as the uncatalysed reaction. (wikipedia.org)
  • Peptidylglycine a-amidating monooxygenase (PAM) is the single known enzyme responsible for catalyzing this reaction. (usf.edu)
  • Based on this 1:1 molar ratio, several novel spectrophotometric, fluorescent, and chemi-luminescent enzyme linked assays for glyoxylate were developed, which when utilized on cell culture fractions proved positive for the identification of a-amidated hormones. (usf.edu)
  • The assay is based on an enzyme-coupled reaction that detects inorganic phosphate. (immunochemistry.com)
  • The new Transcreener cGAMP cGAS Assay directly measures the product cGAMP, in a cGAS enzymatic reaction allowing researchers to screen vast compound libraries for modulators of the enzyme. (labbulletin.com)
  • Rac1 enzyme is not included in the inhibitor screening assay kit. (bellbrooklabs.com)
  • This assay is designed to be used with purified enzyme preparations. (bellbrooklabs.com)
  • The Transcreener Rac1 Assay determines Rac1 enzyme activity by directly measuring the GDP formed by the enzyme using the Transcreener GDP Assay. (bellbrooklabs.com)
  • By detecting GDP output, the assay provides a universal method to assess the activity of any GDP-producing enzyme in real time. (bellbrooklabs.com)
  • Perform your enzyme reaction, add the detection reagents, and measure. (bellbrooklabs.com)
  • Run your enzyme reaction, add Transcreener reagents, and read your plates. (bellbrooklabs.com)
  • The assay conditions below are chosen to generate the best assay window and ability to study the enzyme. (bellbrooklabs.com)
  • The Rac1 enzyme reaction took place for 60, 120, and 180 minutes. (bellbrooklabs.com)
  • PNPC can be synthesized via several methods, including the reaction of p-nitrophenol and cellobiose in the presence of acid or catalytic amounts of an enzyme. (chemsynlab.com)
  • This kit was based on sandwich enzyme-linked immunosorbent assay technology. (bioconstructor.com)
  • Damage to proteins can arise from the reactions of radicals (reactive species with an unpaired electron) and other oxidants, which are generated in biological systems by both endogenous processes (e.g. metabolic pathways, inflammation and enzyme reactions) and also exposure to a wide range of external factors (UV and high-energy radiation, exposure to drugs, pollutants, mineral fibres, chemicals etc). (ku.dk)
  • Small changes (red) to enzyme substrates such as adenosine dramatically reduced enzyme activity, even when the changes were far from the site where an enzymatic reaction occurs. (acs.org)
  • They belong to the class of enzymes known as hydrolases, which catalyse the reaction of hydrolysis of various bonds with the participation of a water molecule. (stratech.co.uk)
  • PNPC can be analyzed through enzymatic assays that monitor the rate of cellobiose hydrolysis using spectrophotometric techniques. (chemsynlab.com)
  • The use of PNPC and related substrates to study the roles of cellobiases and β-glycosides in biological systems beyond cellobiose hydrolysis. (chemsynlab.com)
  • Enzymatic Hydrolysis of Human Milk Oligosaccharides. (iqs.edu)
  • Degradation products derived from the enzymatic hydrolysis of type 1 collagen, particularly peptides related to regions of cross-linking with PYD, are the best markers of bone resorption. (medscape.com)
  • Epiphytic bacteria isolated from plum tissue was used to further evaluate the specificity of the assay. (qld.gov.au)
  • This assay has high sensitivity and excellent specificity for the detection of IgG. (bioconstructor.com)
  • The enzymatic route wins this application due to high selectivity and specificity of enzymatic reactions. (infinitabiotech.com)
  • Development of enzymatic assays for the characterization of the acceptor specificity of thermostable ß - glucosidases. (lu.se)
  • Although urinary hydroxyproline was previously one of the primary bone resorption markers used, specificity and sensitivity were lacking in this assay. (medscape.com)
  • A real-time SYBR Green I assay was developed and evaluated as a biological and enzymatic polymerase chain reaction (Bio-PCR) protocol for the detection of Xanthomonas arboricola pv. (qld.gov.au)
  • Ella is used in the lab for the characterization of biological fluids and for the detection of markers present in extracellular vesicles samples. (labion.eu)
  • The detection assays need to be extremely sensitive and selective in small value of intricate RNA samples. (unboundmedicine.com)
  • The surface plasmon resonance (SPR), an optical biosensor, possessing various advantages including excellent reliability, selectivity and reproducibility represents a wide range of applications in real-time monitoring of biomolecular interactions and detection of biological and chemical analytes with label-based or label free form. (unboundmedicine.com)
  • The Transcreener cGAMP cGAS Assay directly detects cyclic GMP-AMP (cGAMP), the product of a cGAS reaction, allowing researchers to monitor cGAS activity with simple, mix-and-read fluorescent detection. (labbulletin.com)
  • One problem with conventional multiplex assays is that they typically cannot detect more than about five analytes simultaneously, because of difficulties with simultaneous detection and differentiation of more than about five analytes. (justia.com)
  • Protease assays are widely used for the investigation of protease inhibitors and the detection of protease activities. (stratech.co.uk)
  • The Detection Mix was added with the substrate to start the reaction and incubated at 30°C. Readings were taken with a CLARIOstar plate reader. (bellbrooklabs.com)
  • The concentration gradients of the kit standards or positive controls generate a theoretical detection range of the kit in biological research samples containing IgG. (bioconstructor.com)
  • The Nitric Oxide Detection Kit is a colorimetric assay for the indirect determination of Nitric Oxide concentrations based on the enzymatic conversion of nitrate to nitrite by a nitrate reductase. (ozbiosciences.com)
  • The ab65342 Pyruvate (Colorimetric / Fluorometric) Assay Kit provides a simple, straightforward, automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc. (pcrtechnique.com)
  • The importance of appropriate normalization controls in quantitative real-time polymerase chain reaction (qPCR) experiments has become more apparent as the number of biological studies using this methodology has increased. (biomedcentral.com)
  • The analysis of gene expression using quantitative real-time polymerase chain reaction (qPCR) has become increasingly important as biological research has focused on developing insights into the complex regulatory networks that exist within cells [ 1 ]. (biomedcentral.com)
  • In the second season, blood clots ( n = 544) were screened for viral presence by polymerase chain reaction (PCR) for alphaviral and flaviviral RNA, and virus isolation ( n = 146) was conducted. (who.int)
  • Phosphorus is essential for multiple and diverse biological functions, including cellular signal transduction, mineral meatbolism, and energy exchange. (immunochemistry.com)
  • Proteins are the primary class of biological macromolecules and serve to carry out most cellular functions. (columbia.edu)
  • The assay performance behind that data includes sub-picogram level sensitivity, with high reproducibility. (labion.eu)
  • Although it suffers from the intrinsic low sensitivity compared to other biophysical techniques usually applied in functional assays, it has some distinctive features that makes it appealing for tackling complex chemical and biological systems. (novartis.com)
  • The electrocatalytic activity of the carbon nanotubes, enable the sensor to detect electroactive species that are produced from enzymatic reactions with high sensitivity. (cdc.gov)
  • The sensitivity and linearity of the reactions were determined using a regression coefficient. (czytelniamedyczna.pl)
  • HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. (eaglebio.com)
  • Simple, rapid and sensitive analytical sensors are needed to evaluate exposures to organophosphorus insecticides and nerve agents utilizing readily obtainable biological fluids, like saliva. (cdc.gov)
  • This assay is based on the reaction originally described by Griess to detect NO 2 - in a variety of biological fluids such as cell culture medium, plasma, serum, or urine. (ozbiosciences.com)
  • Through the use of a novel DNA polymerase for the primer extension, which preferably incorporates ddNTPs over dNTPs, an enzymatic degradation of residual dNTPs from the PCR is not required. (mdc-berlin.de)
  • Because of the "green chemistry" characterised by enzymatic catalysis, these reactions can be used to produce food grade products while avoiding residues of harmful substances. (infinitabiotech.com)
  • The amount of ammonia in collected blood, urine, saliva, or other biological fluid samples can be affected by several mechanisms that may lead to erroneous ammonia concentration determinations. (cdc.gov)
  • Multiplex assays are performed to determine simultaneously the presence or concentration of more than one molecule in the sample being analyzed, or alternatively, several characteristics of a single molecule, such as, the presence of several epitopes on a single protein molecule. (justia.com)
  • The electrochemical properties of ChE enzymatic products were studied using the sensor, and the operation parameters such as the applied potential and substrate concentration were optimized. (cdc.gov)
  • Particle-enhanced assays are based on the reaction between a soluble analyte and the corresponding antigen or antibody bound to polystyrene particles. (cdc.gov)
  • This assay employs a monoclonal antibody specific for rat IL-6 coated on a 96-well plate. (biovision.com)
  • Actually, label-based signal amplification strategies of SPR platforms including nanoparticle enhancement, supersandwich assembly, streptavidin/biotin complex, antibody amplification, enzymatic reactions, triplex structure formation and catalytic hairpin assembly are discussed. (unboundmedicine.com)
  • The Transcreener cGAMP cGAS Assay is a competitive immunoassay that uses a highly specific antibody for cGAMP and a fluorescent tracer. (labbulletin.com)
  • Transcreener Rac1 Assay technology uses a simple but highly effective method that consists of an antibody selective to GDP and a far-red fluorescent tracer. (bellbrooklabs.com)
  • Assay standards, controls and prediluted patient serum samples containing human Fetuin-A is added to microtiter wells of microplate that was coated with a high affinity polyclonal goat anti-human Fetuin-A antibody. (eaglebio.com)
  • The enzymatic activity of the tracer antibody bound to the Fetuin-A on the wall of the microtiter well is directly proportional to the amount of Fetuin-A in the sample. (eaglebio.com)
  • The NTX assay employs a monoclonal antibody directed against a urinary pool of collagen cross-links (originating from a patient with Paget disease). (medscape.com)
  • Topics covered will include high-throughput assay development, screening, chemical library creation, high-throughput chemistry, affinity purification of target proteins and target validation, protein microarrays, molecular evolution, protein engineering and synthetic biology. (columbia.edu)
  • Amplite™ Universal Fluorimetric Protease Activity Assay Kit is an ideal choice to perform routine protease isolation assays or for identifying the presence of contaminating proteases in protein samples. (stratech.co.uk)
  • β-Ketoacyl-(acyl carrier protein) reductase (FabG) catalyzes the key reductive reaction in the elongation cycle of fatty acid synthesis (FAS), which is a vital metabolic pathway in bacteria and a promising target for new antibiotic development. (rcsb.org)
  • Here we report the findings of structural, enzymatic, and binding studies of the FabG protein found in the causative agent of cholera, Vibrio cholerae (vcFabG). (rcsb.org)
  • Her professional interests include protein structure and function, catalytic mechanisms of enzymatic reactions, sequencing technologies, purification technologies, and all things microbiology and marine biology. (pcrassays.com)
  • Our group works to understand the mechanisms of protein modification by reactive species (radicals, two-electron oxidants, glycation reactions), the biological consequences of such reactions, and the development of methods to quantify protein damage in disease with a particular emphasis on cardiovascular pathologies. (ku.dk)
  • Unfortunately, problems attributed to the biological and technical variability which can occur between the different steps of the experimental procedures, are associated with the qPCR assay. (biomedcentral.com)
  • The molecular biological procedure of the GOOD assays is based on the principle that the analysis of DNA by matrix-assisted laser desorption/ionization (MALDI) is strongly dependent on the charge state. (mdc-berlin.de)
  • BellBrook Labs is dedicated to accelerating drug discovery and biological research by providing innovative high throughput screening solutions. (labbulletin.com)
  • The potential spectrum of bioterrorism ranges from hoaxes and actual use of agents by individuals or groups against others, to state-sponsored terrorism that employs biological warfare (BW) agents and delivery systems that can produce mass casualties. (medscape.com)
  • Enzymatic analysis of cyclic 3', 5'-AMP in mammalian tissues and urine", Anal. (everypatent.com)
  • Adenylate Cyclase Assay", Adv. Cyclic Nucleotide Res. (everypatent.com)
  • Serum CrossLaps assay measures only β-isomer of CTX, while the urine CrossLaps assay measures α- and β-isomers of CTX. (medscape.com)
  • Urine from healthy individuals has produced a detectable reaction from these assays, and elevated turnover has produced large increases. (medscape.com)
  • In other words, the number of different analytes that may be assayed in a single solid phase assay is limited by the solid phase. (justia.com)
  • The assay utilizes a fluorescent casein conjugate that is proven to be a generic substrate for a broad spectrum of proteases (e.g. trypsin, chymotrypsin, thermolysin, proteinase K, protease XIV, and elastase). (stratech.co.uk)
  • The assay utilizes the two-site "sandwich" technique with two selected goat anti-human Fetuin-A polyclonal antibodies that bind to different epitopes of human Fetuin-A. (eaglebio.com)
  • By complex cooperative action the proteases may proceed as cascade reactions, which result in rapid and efficient amplification of an organism's response to a physiological signal. (stratech.co.uk)
  • To facilitate the accurate quantification of total bile acids in serum and plasma, the 5th Generation Total Bile Acids Assay has emerged as a reliable and advanced diagnostic tool. (randox.com)
  • Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples. (bellbrooklabs.com)
  • The synthesis of PNPC can also be achieved using in vitro enzymatic synthesis. (chemsynlab.com)
  • Recent research has focused on the development of more sensitive and specific cellobiase assays using PNPC, as well as the synthesis of modified PNPC derivatives that can be used to study other β-glycosidases. (chemsynlab.com)
  • For the pharmaceutical sector, the activities dedicated to the analysis, synthesis and biological evaluation of small bioactive organic molecules are able to support studies of lead compounds and preclinical development of lipids, natural products, peptides and biomarkers for oncology, immunology and neuroscience. (cnr.it)
  • These assays are performed on a Behring Nephelometer for quantitative CRP determination. (cdc.gov)
  • Procedures for the determination of ammonia in biological samples are found in Table 7-1. (cdc.gov)
  • On the other hand we also assay the nutrients such as glucose, ammonia, phosphorus and protease during the fermentation. (ukessays.com)
  • Monitoring various protease activities has become a routine task for many biological laboratories. (stratech.co.uk)
  • Along with basic cGAS research including enzymology and regulation, the new assay is designed for miniaturized 384 or 1536 well formats, perfect for HTS. (labbulletin.com)
  • The Rac1 assay is compatible with 96, 384, and 1536-well formats. (bellbrooklabs.com)
  • These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to highly regulated cascades (e.g., the blood-clotting cascade, the complement system, apoptosis pathways, and the invertebrate prophenoloxidase-activating cascade). (stratech.co.uk)
  • Bioenergy: Cellobiases are critical enzymes in the production of biofuels from lignocellulosic biomass, and PNPC can be used to develop and optimize cellobiase assays for this purpose. (chemsynlab.com)
  • In the biotechnology field, the Institute conducts research in the development and implementation of processes based on extremophilic and whole-cell enzymes for the biological mitigation of CO 2 and for the production of hydrogen, food ingredients and functional and/or bioactive compounds from the conversion of natural matrices and residues agro-food. (cnr.it)
  • The transformation of milk to cheese, grains to alcohol, fruits to juice, and flour to bread all rely on the reactions catalysed by enzymes. (infinitabiotech.com)
  • As enzymes are specific biological catalysts, they should make the most desirable therapeutic agents for the treatment of metabolic diseases. (infinitabiotech.com)
  • A highly sensitive adenylate cyclase assay", Analy. (everypatent.com)
  • Inhibitors present in both water and soils RXDX-106 are present in watershed run-off and our method was optimized so that the assay was just as sensitive as the use of pure cultures in PBS. (proteasomesignaling.com)
  • Our incubation assays demonstrate methane production that is highly sensitive to the Mcr inhibitor bromoethanesulfonate, stimulated by xyloglucan and xyloglucan-derived sugars, concomitant with the consumption of molecular hydrogen, and causing a deuterium fractionation in methane characteristic of hydrogenotrophic and methylotrophic methanogens. (stanford.edu)
  • Reactions with three or four substrates or products are less common, but they exist. (wikipedia.org)
  • TMB substrates were used to visualize the enzymatic reaction of HRP. (bioconstructor.com)
  • The functions of the aforementioned residues were confirmed by binding and enzymatic assays for the corresponding mutants. (rcsb.org)
  • The assay demonstrates linearity when raw data is converted to GDP using a standard curve. (bellbrooklabs.com)
  • After having added sample and buffers and loaded the cartridge into the instrument, in just 90 minutes Ella gives us highly reproducible validated assay data with no manual steps. (labion.eu)
  • The technical variables include the amount of starting materials in the reactions, the quality of the RNA samples and the efficiency of the enzymatic steps (i.e. reverse transcription and PCR) [ 5 , 6 ]. (biomedcentral.com)
  • This invention relates to materials and methods for detecting nucleic acids in samples and, more particularly, to solid phase assays wherein transponders are associated with the beads constituting the solid phase, nucleic acid probes are bound to the surface of the particles and data concerning the assay is encoded on the transponders. (justia.com)
  • Selection was based on availability of a previously signed informed consent allowing for biopsy samples banking and future research usage and availability of sufficient biological material without compromising further analyses if necessary. (biomedcentral.com)
  • GDP produced in the reaction competes with the tracer changing the fluorescent properties and providing fluorescent readout. (bellbrooklabs.com)
  • The primary such as hs-CRP, total homocysteine, as well enzymatic defences are intracellular, but as oxidative stress, have been proposed other antioxidant defences are largely ex- as risk factors for the development and tracellular, including antioxidative sub- progression of atherosclerosis and athero- strates such as uric acid and unconjugated thrombotic cardiovascular disease [10-13]. (who.int)
  • This example assumes the simplest case of a reaction with one substrate and one product. (wikipedia.org)
  • The substrate is converted to Hydrogen peroxide in presence of inorganic phosphate in a coupled enzymatic reaction. (immunochemistry.com)
  • Transcreener GDP Assay directly measures GDP produced by Rac1 (Ras-related C3 botulinum toxin substrate 1). (bellbrooklabs.com)
  • We will discuss the structure and function of both proteins and small molecules in biological systems. (columbia.edu)
  • In conventional solid-phase assays, the solid phase mainly aids in separating molecules that bind to the solid phase from molecules that do not bind to the solid phase. (justia.com)
  • The Institute has a proven experience in analyzing, preparing and evaluating the biological properties of small organic molecules. (cnr.it)
  • Automated platform properly suited for small molecules screening with immunomodulatory activity that relies on phenotypic assays using a range of cancer cells, reporter cells, humanized cells, primary nervous cells and immune system cells, animal models with invertebrates (zebrafish) and mammals, as well as on an instrument park for microscopy, fluorescence, mass imaging and mass cytometry. (cnr.it)
  • We invest time in the interpretation of complex spectral data to identify our compounds from natural sources, developing biological models to demonstrate their efficacy on health and understanding their biological role, implementing synthetic and biosynthetic pathways for their sustainable production. (cnr.it)
  • More specifically, we suspected that the products of the oxidative wtIDH1 reaction, NADPH and αKG, directly power antioxidant defense and mitochondrial function to promote PDAC survival. (nature.com)
  • Then the unmodified part of the primers is digested by phosphodiesterase II and the negative charges of the phosphorothioates are neutralized by an alkylation reaction resulting in charge-tagged DNA products. (mdc-berlin.de)
  • Optical isomers containing both R- and S- configurations are often present as the final products of chemical catalysed reactions. (infinitabiotech.com)
  • miRNAs are a large family of non-coding RNAs which play important roles in translational and post-transcriptional regulation of gene expression and biological processes. (unboundmedicine.com)
  • The course is intended to provide a foundation needed for advanced chemical biology research, i.e. the creation and use of chemical probes of biological processes and macromolecular function. (columbia.edu)
  • In the pyruvate assay protocol, pyruvate oxidase is oxidized by enzymatic reactions to generate color (λ = 570 nm) and fluorescence (at Ex / Em = 535/587 nm). (pcrtechnique.com)
  • Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)
  • Identification of a-amidated hormones from a biological source has been severely hindered by the lack of a specific assay for this distinctive class of biological hormones, indicating that numerous a-amidated hormones remain undiscovered. (usf.edu)
  • iFOUR Dispensing System of M24You is an automatic instrument for handling of small amounts of chemical or biological solutions or suspensions. (labion.eu)
  • We will discuss approaches for discovering and optimizing chemical tools for measuring and perturbing biological systems. (columbia.edu)
  • This was the first multilateral agreement that extended prohibition of chemical agents to biological agents. (medscape.com)
  • We further sequenced these cell lines using long-read technology on an Oxford Nanopore PromethION and here compare its performance alongside more common chemical/enzymatic conversion kits and short-read sequencing. (biomedcentral.com)
  • Our aim was to compare real life operational characteristics for three EGFR mutation assays - two targeted approaches and a next generation sequencing (NGS) technique. (biomedcentral.com)
  • I combine fieldwork and in-situ experiments with laboratory analyses and apply cutting-edge geochemical and molecular biological techniques, including isotopic tracers, DNA and RNA analysis, gene tree/species tree reconciliation and ancestral character state reconstruction, fluorescence in-situ hybridization (FISH), and microbiological culturing. (stanford.edu)
  • The research on the auxiliary role of reducing factors revealed that reducing sulfur compounds could be applied in keratinolysis enhancement during enzymatic digestion of keratin, rather than in culture conditions. (scielo.br)
  • Based on the proven Transcreener platform, trusted for over 10 years by pharmaceutical companies around the globe, the assay has been extensively validated for HTS, SAR, and MOA studies with human and mouse cGAS in BellBrook's internal discovery program. (labbulletin.com)
  • The German-American physician Anton Dilger established a secret biological laboratory in Chevy Chase, Maryland, with the intent to grow the causative agents of anthrax and glanders. (medscape.com)
  • Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 certified laboratory. (bioconstructor.com)
  • Each sample is processed in multiple replicates by three whole-genome bisulfite sequencing (WGBS) protocols (TruSeq DNA methylation, Accel-NGS MethylSeq, and SPLAT), oxidative bisulfite sequencing (TrueMethyl), enzymatic deamination method (EMSeq), targeted methylation sequencing (Illumina Methyl Capture EPIC), single-molecule long-read nanopore sequencing from Oxford Nanopore Technologies, and 850k Illumina methylation arrays. (biomedcentral.com)
  • The preeminent source of biological methane on Earth is methyl coenzyme M reductase (Mcr)-dependent archaeal methanogenesis. (stanford.edu)
  • Easy, convenient and time-saving method to assay for rat IL-6. (biovision.com)
  • Paraoxon (0.7 nM and 7 nM) was mixed with 10-fold diluted saliva (1:1 v/v) and incubated for different time periods (5 to 120 min) then 100 microL of the mixed solution was reacted with an equal volume of 5 mM acetylthiocholine (approximately 10 min) and sequentially assayed with the sensor. (cdc.gov)
  • Disclosed are materials and methods for performing multiplex assays for nucleic acids, in which a transponder is associated with the bead(s) forming the solid phase used in the assay, nucleic acid probes are bound to the surface of the particles, and data concerning the assay is encoded on the transponder. (justia.com)
  • Only the GOOD assay does not need any sample purification. (mdc-berlin.de)
  • Enzymatic assays can also be coupled with additional techniques like liquid chromatography-mass spectrometry (LC-MS) and capillary electrophoresis (CE) to obtain more detailed information about cellobiase activity. (chemsynlab.com)
  • The lipid peroxidation (TBARS) and the enzymatic activity of catalase (CAT) were measured in the cardiac muscle. (bvsalud.org)
  • In a 2.50 mL reaction mix, the final concentrations are 105.6 mM potassium phosphate, 14.4 mM sodium phosphate, 1.2 mM ethylenediamine tetraacetic acid, 2.4 mM L-cysteine, 0.07% (v/v) Brij 35, 0.006 mM Nα-CBZ-Arg-Arg-7-amido-4-methylcoumarin, 0.0525% (v/v) dimethyl sulfoxide, and 0.2 - 0.4 units of Cathepsin B. (sigmaaldrich.com)
  • Assays have been developed based on specific antibodies raised in an immune response against isolated collagen peptides containing cross-links. (medscape.com)