• Low density BCE cell cultures maintained on extracellular matrix (ECM)-coated dishes and plated in the total absence of serum proliferate actively when exposed to a synthetic medium supplemented with high density lipoprotein (HDL 500 micrograms protein/ml), transferrin (10 micrograms/ml), insulin (5 micrograms/ml), and fibroblast (FGF) or epidermal growth factor (EGF) added at concentrations of 100 or 50 ng/ml, respectively. (nih.gov)
  • Glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1 (GPI-HBP1) also known as high density lipoprotein-binding protein 1 is a protein that in humans is encoded by the GPIHBP1 gene. (wikipedia.org)
  • Hyperlipidemia comprises a heterogeneous group of disorders, characterized by high levels in one or more lipids and/or lipoproteins [atherogenic free fatty acids (FA), triglycerides (TG) (hypertriglyceridemia), small dense low-density lipoprotein cholesterol (LDL-C) (hypercholesterolemia), and apolipoprotein (apo) B], and/or low level in antiatherogenic high density lipoprotein cholesterol (HDL-C), in the circulation [ 1 - 3 ]. (hindawi.com)
  • Serum lipids profiles, including total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG), apolipoprotein A-Ⅰ (apoA-Ⅰ), apolipoprotein B (apoB) and clinicopathologic data were analyzed. (researchsquare.com)
  • 15] found that oxidized low-density lipoprotein (ox-LDL) in serum was higher in ovarian cancer patients than in control subjects, but total cholesterol (TC) and high-density lipoprotein (HDL) showed no such association. (researchsquare.com)
  • Serum amyloid P component associates with high density lipoprotein as well as very low density lipoprotein but not with low density lipoprotein. (researchmap.jp)
  • Albumin-sized tracers dominantly crossed the cultured endothelium via junctions between neighbouring cells, high-density-lipoprotein-sized tracers crossed at tricelluar junctions whilst low-density-lipoprotein-sized tracers crossed through cells. (imperial.ac.uk)
  • In the present studies we have examined the impact of LDL carotenoid content on its oxidation by human aortic endothelial cells (EaHy-1) in culture, comparing the effects of in vivo supplementation with in vitro enrichments. (eurekamag.com)
  • Isolated sinusoidal endothelial cells from the rat liver displayed saturable, high affinity binding of AcAc LDL (Kd = 2.5 X 10(-9) M at 4 degrees C), and were shown to degrade AcAc LDL 10 times more effectively than aortic endothelial cells. (rupress.org)
  • Oxidized low density lipoprotein inhibits the migration of aortic endothelial cells in vitro. (rupress.org)
  • The direct effects of CNT were evaluated in vitro in human aortic endothelial cells (HAEC). (cdc.gov)
  • Our previous studies showed that in vitro enrichment of low-density lipoprotein (LDL) with beta-carotene protected it from cell-mediated oxidation. (eurekamag.com)
  • Because macrophages, Kupffer cells, and to a lesser extent, endothelial cells metabolize these modified lipoproteins in vitro, it was of interest to determine whether endothelial cells or macrophages could be responsible for the in vivo uptake of these lipoproteins. (rupress.org)
  • Here, we studied the contribution of Ca2+-independent phospholipase A(2) (iPLA(2)), and the role of the MAP kinase family as well as both cPLA(2) and iPLA(2) mRNA expression by RT-PCR in oxLDL toxicity to GP8.39 cells in vitro. (unict.it)
  • The in vitro model consists of a coculture of bovine brain capillary endothelial cells (BBCEC) and rat glial cells. (aspetjournals.org)
  • The group uses in vitro, ex vivo and in vivo cell and molecular approaches to delineate the signalling pathways controlling gene expression and functional responses of human and murine large vessel and microvascular endothelial cells.Caroline was an associate editor of the Biochemical Journal for over 10 years and is a founder member of the European Vascular Biology Organisation and of the London Vascular Biology Forum. (rvc.ac.uk)
  • The aim of the present study was to identify whether P. gingivalis heat‑shock protein 60 (HSP60) induced the dysfunction of human umbilical vein endothelial cells (HUVECs) in vitro. (spandidos-publications.com)
  • Determine the effect of diets differing in fat and carbohydrate type on cardiometabolic risk indicators, lipoprotein and fatty acid metabolism, response to lipid modifying therapy, and gene-nutrient interactions, using human, animal and in vitro models. (usda.gov)
  • 1.1 - Relative effects of palmitate (16:0), stearate (18:0) and oleate (18:1) on cardiometabolic risk factors, fatty acid kinetics and lipoprotein-mediated in vitro endothelial cell inflammatory response. (usda.gov)
  • Systemic autoimmune diseases, such as rheumatoid arthritis (RA), exhibit accelerated atherosclerosis (AS) [ 1 - 4 ] as a consequence of endothelial dysfunction, leading to higher incidence of cardiovascular (CV) disease (at least 2-fold enhanced CV risk) and premature and higher mortality [ 5 , 6 ]. (hindawi.com)
  • SAA was shown to exhibit causal properties in AS, as a consequence of endothelial dysfunction (elevating tissue factor, as well as a variety of cytokines/chemokines) and early lesions (biglycan synthesis) [ 15 ] to plaque destabilization by inducing matrix metalloproteinases [ 16 ]. (hindawi.com)
  • Endothelial dysfunction during diabetes has been previously reported to be at least in part attributed to increased oxidized low‑density lipoprotein (oxLDL) levels mediated by high glucose (HG) levels. (spandidos-publications.com)
  • These results suggest that rosmarinic acid can exert a protective effect against oxLDL‑mediated endothelial dysfunction under HG conditions by reducing the interaction between monocytes and ECs in addition to preventing monocyte diapedesis. (spandidos-publications.com)
  • A number of studies have previously suggested that vascular endothelial dysfunction caused by increased junctional permeability is a key event in the development and progression of atherosclerosis and atherosclerotic cardiovascular diseases ( 3 , 4 ). (spandidos-publications.com)
  • Chronic metabolic disorders, such as hyperglycemia and dyslipidemia, promote endothelial dysfunction and eventually leakage, resulting in increased monocyte diapedesis ( 5 - 7 ). (spandidos-publications.com)
  • Endothelial dysfunction during diabetes is closely associated with increased oxidized low-density lipoprotein (oxLDL) levels mediated by high glucose (HG) conditions ( 8 ). (spandidos-publications.com)
  • The TGRLs only caused inflammation when exposed to this immune molecule, which suggests that people with existing low-grade inflammation may be more susceptible to endothelial dysfunction related to triglyceride "spikes" that occur after eating high-fat meals, Passerini said. (ucdavis.edu)
  • Smoking is a major risk factor for bronchopulmonary diseases, and it plays an important role in endothelial dysfunction development. (intechopen.com)
  • Chronic exposure to tobacco smoke and the products of combustion of tobacco leads to chronic system inflammatory reaction, oxidative stress, endothelial dysfunction and morpho-functional damage of target organs. (intechopen.com)
  • Studying the mechanisms of endothelial dysfunction in brain blood vessels of patients with smoking habits and COPD can be very important for preventing acute vascular events. (intechopen.com)
  • This study was designed to investigate the effects of substances released from non-injured and injured bovine arterial endothelial cells on 125I-low density lipoprotein uptake and degradation by smooth muscle cells in culture. (lu.se)
  • It was demonstrated that endothelial cell-released non-dialysable (molecular weight cut off 12-14000) substances significantly stimulated 125I-low density lipoprotein uptake and degradation by smooth muscle cells. (lu.se)
  • The increase in 125I-low density lipoprotein uptake and degradation by smooth muscle cells could be dissociated from cell proliferation. (lu.se)
  • However, in endothelial cell-smooth muscle cell co-culture 125I-low density lipoprotein uptake and degradation by smooth muscle cells were not stimulated. (lu.se)
  • Injury to endothelial cells by lipid-soluble smoke particles or ultraviolet light, which reduced total cellular protein by 15-25%, enhanced the endothelial cell release of the substances stimulating 125I-low density lipoprotein uptake. (lu.se)
  • article{e328883f-12c1-4d3a-a8e7-8700ca87f7ac, abstract = {{This study was designed to investigate the effects of substances released from non-injured and injured bovine arterial endothelial cells on 125I-low density lipoprotein uptake and degradation by smooth muscle cells in culture. (lu.se)
  • Carotenoids may protect low-density lipoprotein from oxidation, a process implicated in the development of atherosclerosis. (eurekamag.com)
  • Uptake of chemically modified low density lipoproteins in vivo is mediated by specific endothelial cells. (rupress.org)
  • Acetoacetylated (AcAc) and acetylated (Ac) low density lipoproteins (LDL) are rapidly cleared from the plasma (t1/2 approximately equal to 1 min). (rupress.org)
  • Compelling evidence suggests that oxidized low density lipoprotein (LDL) is present in atherosclerotic lesions, but its role in lesion formation has not been defined. (rupress.org)
  • Atherogenic lipoproteins [for example, low-density lipoprotein (LDL) and oxidized LDL (ox-LDL)] have been shown to stimulate glomerular monocyte chemoattractants involved in monocyte infiltration. (ewha.ac.kr)
  • Omission of any of these components results in a lower growth rate and/or final cell density of the cultures. (nih.gov)
  • LIPG is a carboxyl esterase that hydrolyzes high density lipoproteins, emulsifies triglycerides, is essential for the efficient digestion of dietary fats and can bind to heparin. (thermofisher.com)
  • It involves interactions between oxidized low-density lipoprotein (LDL) deposits, endothelial cells, leukocytes, monocytes, macrophages, T-lymphocytes and smooth muscle cells, all of which are involved in promoting cellular migration and proliferation to drive the formation of foam cell clusters abundant in atherosclerotic plaque [ 4 ]. (springer.com)
  • Arterial inflammation that precedes development of the atherosclerotic plaque is caused by accumulation of LDL (low density lipoproteins) in the extracellular matrix of the blood vessels. (europa.eu)
  • To investigate the simultaneous presence of macrophages, CRP, membrane attack complex C5b-9 (MAC), and oxidised low density lipoprotein (oxLDL) in atherectomy specimens from patients with different coronary syndromes. (bmj.com)
  • 4 Furthermore, CRP promotes foam cell formation by opsonising low density lipoprotein (LDL). (bmj.com)
  • CNT also dose-dependently induced low density lipoproteins (LDL) oxidation in the presence of HAECs. (cdc.gov)
  • Retraction Note: SNHG6 modulates oxidized low-density lipoprotein-induced endothelial cells injury through miR-135a-5p/ROCK in atherosclerosis. (nih.gov)
  • Additionally, P. gingivalis induces macrophage foam cell formation ( 11 ) and stimulates oxidation of low-density lipoprotein ( 12 ). (spandidos-publications.com)
  • Autocrine FGF-2 is responsible for the cell density-dependent susceptibility to apoptosis of HUVEC : a role of a calpain inhibitor-sensitive mechanism. (researchmap.jp)
  • Oxidized low density lipoprotein induces apoptosis in cultured human umbilicalvein endothelial cells by common and unique mechanisms. (researchmap.jp)
  • The formation of atherosclerotic lesions involves increased permeability of endothelium to plasma macromolecules (eg, low-density lipoprotein [LDL]), increased turnover of endothelial cells and smooth muscle cells, and increased numbers of intimal macrophages. (medscape.com)
  • Low-density lipoprotein (LDL) oxidation is central to the pathogenesis of atherosclerosis. (mapi.com)
  • We explore several physiologically relevant functions of ESC-derived EC (ESC-EC), such as its capacity to produce nitric oxide (NO), regulate permeability, activate and express surface molecules for the recruitment of leukocytes in response to inflammatory stimuli, migrate and grow new blood vessels, lay down extracellular matrix, and take up low-density lipoproteins. (ca.gov)
  • Scavenger receptors are expressed by MYELOID CELLS and some ENDOTHELIAL CELLS, and were originally characterized based on their ability to bind acetylated LOW-DENSITY LIPOPROTEINS. (bvsalud.org)
  • Abnormalities in lipid and lipoprotein metabolism have been implicated in the pathogenesis of glomerulosclerosis. (ewha.ac.kr)
  • Plasma lipoprotein, plasma lipid peroxide, and LDL oxidation studies were performed every 6 weeks. (mapi.com)
  • Some experiments prove that aggressive pollutants of tobacco smoke (benzopyrene, peroxynitrite, acrolein, cyanides, peroxides, etc.) can cause direct damage to endothelial cells due to expression of adhesion molecules on their surface and intensification of lipid peroxidation. (intechopen.com)
  • Processes involved in atherosclerosis include coagulation, inflammation, lipid metabolism, intimal injury, and smooth muscle cell proliferation (see the image below). (medscape.com)
  • Thus, citric acid cycle intermedi- ates are not used for adenosine triphosphate (ATP) production and are shuttled out of the mitochondria, providing precursors for nucleotide, amino acid, and lipid synthesis path- ways for the dividing cell [13]. (who.int)
  • Together, these data link the stimulation of PKC-ERK-p38-JNK pathways and PLA(2) activity by oxLDL to the prooxidant mechanism of the lipoprotein complex, which may initially stimulate the endothelial cell reaction against noxious stimuli as well as metabolic repair, such as during inflammation and atherosclerosis. (unict.it)
  • Endothelial inflammation increases the adhesiveness of monocytes to the endothelium in addition to increasing vascular permeability, promoting diabetic atherogenesis. (spandidos-publications.com)
  • Triglycerides are types of fat molecules, commonly associated with "bad cholesterol," known to increase risk of inflammation of the endothelium, the layer of cells that lines arteries. (ucdavis.edu)
  • They wanted to learn how triglyceride levels can cause endothelial inflammation, and find a way to assess an individual's inflammatory potential. (ucdavis.edu)
  • These particles can bind to the endothelium, triggering inflammation and an immune response that brings white blood cells to repair the damage. (ucdavis.edu)
  • The researchers tested whether TGRL particles from the volunteers' blood could cause cultured endothelial cells in the laboratory to express markers for inflammation. (ucdavis.edu)
  • There was a mixed response: individuals with both a waist size over 32 inches (not terribly large by most standards) and high triglyceride levels had large lipoprotein particles that bound easily to the endothelial cells and caused inflammation in response to an immune chemical "trigger. (ucdavis.edu)
  • This review summarizes some immunological factors involved in the development and control of this oral disease, such as: the participation of inflammatory cells in local inflammation, the synthesis of chemotaxis proteins with activation of the complement system and a range of antimicrobial peptides, such as defensins, cathelicidin and saposins. (bvsalud.org)
  • These data indicate that specific sinusoidal endothelial cells, not the macrophages of the reticuloendothelial system, are primarily responsible for the removal of these modified lipoproteins from the circulation in vivo. (rupress.org)
  • In particular, it plays a vital role in the physiological and pathological activities of the three important cells, Endothelial cells, macrophages and vascular smooth muscle cells. (wjgnet.com)
  • Methamphetamine Increases the Proportion of SIV-Infected Microglia/Macrophages, Alters Metabolic Pathways, and Elevates Cell Death Pathways: A Single-Cell Analysis. (academictree.org)
  • Interferon gamma suppresses PDGF production from THP-1 cells and blood monocyte-derived macrophages. (researchmap.jp)
  • The data indicate that ox-LDL, by stimulating monocyte adhesion to the glomerular endothelium, may regulate monocyte infiltration within the glomerulus, supporting an early pathobiological role for atherogenic lipoproteins in glomerular injury. (ewha.ac.kr)
  • However, the routes by which macromolecules cross endothelium, the hemodynamic stresses that maintain endothelial physiology or trigger arterial disease, and the dependence of transendothelial transport on hemodynamic stresses are controversial. (imperial.ac.uk)
  • Uptake of AcAc LDL by endothelial cells of the liver, spleen, and bone marrow was confirmed by transmission electron microscopy. (rupress.org)
  • Phosphatidylinositol 3-kinase is required for growth factor-induced amino acid uptake by vascular smooth muscle cells. (researchmap.jp)
  • A large group of structurally diverse cell surface receptors that mediate endocytic uptake of modified LIPOPROTEINS. (bvsalud.org)
  • LIPG most likely plays a physiological role in modulating lipoprotein metabolism. (thermofisher.com)
  • LIPG has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. (thermofisher.com)
  • Context-dependent regulation of endothelial cell metabolism: differential effects of the PPARβ/δ agonist GW0742 and VEGF-A. Sci Rep. 2020 May 12;10(1):7849. (rvc.ac.uk)
  • Concentrations of cholesterol and triglycerides are strongly correlated in the adipocyte, but little is known about mechanisms regulating cholesterol metabolism in fat cells. (jci.org)
  • GPIHBP1 is a capillary endothelial cell protein that provides a platform for LPL-mediated processing of chylomicrons. (wikipedia.org)
  • Aprotinin transcytosis across bovine brain capillary endothelial cell (BBCEC) monolayers is at least 10-fold greater than that of holo-transferrin. (aspetjournals.org)
  • The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)
  • peptide 14 or 19 from P. gingivalis HSP60 may have either an anti- or pro-atherogenic role, respectively, in the ApoE(−/-) mouse model of infection-triggered atherosclerosis through distinct mechanisms operating in the polarization of T cells. (spandidos-publications.com)
  • When atherogenic lipoproteins exceed certain critical levels, the mechanical forces may enhance lipoprotein insudation in these regions, leading to early atheromatous lesions. (medscape.com)
  • Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)
  • After 12 weeks of treatment with MAK-4 and MAK-5 , a time-dependent increase in the lag phase and delay in the propagation phase of oxidation of LDL by Cu+2 and endothelial cells was seen. (mapi.com)
  • Lag phases were 14.89 hours ± 0.77, 13.33 hours ± 0.50, 20.22 hours ± 0.76, and 20.00 hours ± 0.79, respectively, for endothelial cell-induced LDL oxidation. (mapi.com)
  • There is increasing evidence that free fatty acids have the ability to trigger cell apoptosis and it has been shown that exposure of endothelial cells to E-LDL results in programmed cell death. (europa.eu)
  • Thus, in addition to its established role in the sensing and clearance of apoptotic cells, C1q may also regulate fatty acid-induced apoptosis. (europa.eu)
  • Considering the possible implication of endothelial cell apoptosis in the atherogenesis process, such a property would bear important biological implications. (europa.eu)
  • Dietary fats are packaged by intestine into triglyceride-rich lipoproteins called chylomicrons. (wikipedia.org)
  • Passerini's team found that after eating the high-fat meal, the size of a type of a particle called triglyceride-rich lipoprotein (TGRL) varied directly with the individual's waist size and preexisting blood triglyceride level. (ucdavis.edu)
  • However, the role of LDL and ox-LDL in the early monocyte adhesion to glomerular endothelial cells (ECs) and associated intracellular signaling mechanisms are not clearly understood. (ewha.ac.kr)
  • Therefore, the present study aimed to elucidate the mechanisms involved in oxLDL‑induced endothelial permeability and monocyte diapedesis under HG conditions, in addition to the potential effects of rosmarinic acid. (spandidos-publications.com)
  • She is particularly known for her work on mechanisms of vascular endothelial eicosanoid generation and recent studies have identified biological roles for novel fatty acid metabolites in vascular repair and angiogenesis. (rvc.ac.uk)
  • Her research group (funded by the BBSRC, The Wellcome Trust and the British Heart Foundation) is investigating the molecular mechanisms important for regulating pro- and anti-inflammatory gene expression and downstream cellular functions in response to a range of external stimuli, including ligands for G protein-coupled receptors and growth factor receptors, and exogenous lipoproteins. (rvc.ac.uk)
  • focus is primarily on vascular cell function with emphasis on understanding molecular control mechanisms. (rvc.ac.uk)
  • The integration of pathogen-associated molecular patterns (PAMPs) from microorganisms with their surface receptors in the immune cells, induces the production of several cytokines and chemokines that presents either a pro- and/or anti-inflammatory role by stimulating the secretion of a great variety of antibody subtypes and the activation of mechanisms of controlling the disease, such as the regulatory T cells. (bvsalud.org)
  • Her laboratory specialises in investigating the functional relevance of molecular signalling pathways in human and animal endothelial cells and a range of other vascular cell types. (rvc.ac.uk)
  • An elaborate network of signaling pathways orchestrates the communication between cancer cells and the surrounding stroma. (degruyter.com)
  • Once absorbed into the body, tocotrienols are readily taken up by cell membranes where they help neutralize harmful free radicals, help maintain cell membrane fluidity and support cell signaling pathways. (endur.com)
  • A strong inhibition of MAPK activation was also observed when endothelial cells were treated with GF109203X, a PKC inhibitor, indicating the important role of both PKC and all three MAPKs in mediating the maximal oxLDL response. (unict.it)
  • Protein kinase C-mediated inhibition of vascular smooth muscle cell proliferation : The isoform that may mediate G1/S inhibition. (researchmap.jp)
  • Protein kinase C-mediated inhibition of cyclin A expression in human vascular endothelial cells. (researchmap.jp)
  • Confluent cultures of bovine aortic EC were "wounded" with a razor, and migration was measured after 18 to 24 h as the number of cells moving into the wounded area and the mean distance of cells from the wound edge. (rupress.org)
  • Experimental conditions have been defined that allow bovine corneal endothelial (BCE) cells to grow in the complete absence of serum. (nih.gov)
  • Endothelial cell (EC) migration is a critical and initiating event in the formation of new blood vessels and in the repair of injured vessels. (rupress.org)
  • Passerini collaborated with Scott Simon, professor of biomedical engineering at UC Davis, to develop cell culture models to mimic the properties of blood vessels. (ucdavis.edu)
  • Cell membranes throughout the body - from lipoproteins that travel through the blood to endothelial cells that line blood vessels - can benefit from this powerful antioxidant protection. (endur.com)
  • The first report in 2007, on SAA-stimulated human coronary artery endothelial cells (HCAEC), exhibited a substantial and significantly higher induction of released IL-6 protein and mRNA levels as compared to HUVEC [ 17 ], as well as increased responsiveness to IL-1 β [ 18 ]. (hindawi.com)
  • ECs were treated with oxLDL under HG conditions in the presence or absence of ROS scavengers mitoTEMPO and NAC, p38 inhibitor SB203580, FOXO1 inhibitor AS1842856 or transfected with the TXNIP siRNA, before protein expression levels of intercellular adhesion molecule 1 (ICAM‑1), vascular cell adhesion molecule‑1 (VCAM‑1), phosphorylated vascular endothelial‑cadherin (VE‑cadhedrin), VE‑cadherin and zonula occludens‑1 (ZO‑1) were measured by western blotting. (spandidos-publications.com)
  • In addition, oxLDL‑stimulated ECs under HG conditions exhibited increased phosphorylated VE‑cadherin protein levels and decreased ZO‑1 protein expression levels compared with those in untreated ECs, suggesting increased endothelial permeability. (spandidos-publications.com)
  • Full length human recombinant protein of human LIPG produced in HEK293T cell. (thermofisher.com)
  • Protein tyrosine kinase inhibitors inhibit both proliferation and chemotaxis of vascular smooth muscle cells. (researchmap.jp)
  • For this reason, they are extensively used in a wide range of applications from protein production to bacterial biofilms and endothelial cell experiments. (imperial.ac.uk)
  • A GST pull-down assay showed that recombinant SpCrus2 interacted specifically with WSSV structural protein VP26, and this result was further confirmed by a co-immunoprecipitation assay with Drosophila S2 cells. (bvsalud.org)
  • The enhancing effect of ECM on BCE cell growth and culture longevity clearly illustrates the importance of the cell substrate in the control of proliferation of these cells. (nih.gov)
  • Bidirectional regulation of smooth muscle cell proliferation by IFN gamma. (researchmap.jp)
  • Endothelial cells (EC) derived from embryonic stem cells (ESC) require additional functional characterization before they are used as a cell therapy in order to enhance their potential for engraftment and proliferation. (ca.gov)
  • In a previous study, it was reported that oxLDL treatment induced nucleotide‑binding domain and leucine‑rich repeat containing family, pyrin domain‑containing 3 inflammasome activation in endothelial cells (ECs) under HG conditions, in a manner that could be effectively reversed by rosmarinic acid. (spandidos-publications.com)
  • The effects of oxLDL‑mediated inflammasome activation on endothelial permeability under HG conditions, in addition to the effects of rosmarinic acid on these oxLDL‑mediated processes, also remain poorly understood. (spandidos-publications.com)
  • Furthermore, monocyte transmigration through the endothelial monolayer was significantly increased by oxLDL treatment under HG conditions. (spandidos-publications.com)
  • Subsequently, rosmarinic acid treatment significantly reversed oxLDL‑induced overexpression of adhesion molecules and monocyte‑EC adhesion, oxLDL‑induced endothelial junction hyperpermeability and monocyte transmigration through the endothelial monolayer under HG conditions, in a dose‑dependent manner. (spandidos-publications.com)
  • We further characterized the subphenotype of our ESC-EC and observed both venous and arterial markers on individual cells with a larger percentage of the cells exhibiting a venous phenotype. (ca.gov)
  • CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. (novusbio.com)
  • In turn, oxidized lipoproteins in the tunica intima of the vessel work as attractants for chemotaxis of leukocytes and monocytes that start to produce pro-inflammatory cytokines in big amounts. (intechopen.com)
  • Flow Cytometry: SR-BI Antibody [NB400-104] - Analysis of Huh7 and HepG2 cells using SR-B1 antibody NB400-104. (novusbio.com)
  • Endothelial monolayers were cultured under static conditions or on an orbital shaker producing different flow profiles in different parts of the wells. (imperial.ac.uk)
  • Apolipoprotein A, apolipoprotein B, and lipoprotein (a) levels were measured at baseline and 18 weeks. (mapi.com)
  • No significant changes were seen in the plasma lipoproteins and the levels of apolipoprotein A, apolipoprotein B, and lipoprotein (a). (mapi.com)
  • Flow Cytometry: SR-BI Antibody [NB400-104] - An intracellular stain was performed on HeLa cells with SR-BI antibody NB400-104AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). (novusbio.com)
  • Melero-Martin JM , Khan ZA , Picard A , In vivo vasculogenic potential of human blood-derived endothelial progenitor cells . (avma.org)
  • Edwards N , Langford-Smith AWW , Wilkinson FL , Endothelial progenitor cells: new targets for therapeutics for inflammatory conditions with high cardiovascular risk . (avma.org)
  • Based on a set of label-free CARS images of atherosclerotic plaques (i.e. foam cell clusters) from a Watanabe heritable hyperlipidemic rabbit model, we developed an automated pipeline to classify atherosclerotic lesions based on their major morphological features. (springer.com)
  • The results suggest that the activation of specific PTK and associated signaling may, at least in part, play a critical role in ox-LDL-mediated endothelial- monocyte interaction-related events. (ewha.ac.kr)
  • Signal transduction of PDGF-induced chemotaxis of vascular smooth muscle cells. (researchmap.jp)
  • Tyrosine kinase ingibitors inhibit multiple steps of the cell cycle of vascular smooth muscle cells. (researchmap.jp)
  • Human Neonatal and adult vascular smooth muscle cells in culture. (researchmap.jp)
  • Mitogenic activity of interferon gamma on growth-arrested human vascular smooth muscle cells. (researchmap.jp)
  • Prostaglandin A and J arrest the cell cycle of cultured vascular smooth muscle cells without suppression of c-myc expression. (researchmap.jp)
  • Glycolysis is an important metabolic pathway that generates energy in various cells of the blood vessel wall. (wjgnet.com)
  • Likewise, BCE cells maintained on ECM and exposed to a synthetic medium supplemented with optimal concentrations of HDL, transferrin, insulin, and FGF underwent 85 generations, whereas control cultures maintained on plastic could not be passaged. (nih.gov)
  • Las personas que consumen más licopeno en su dieta no parecen tener un riesgo menor de desarrollar diabetes. (medlineplus.gov)
  • Experiments with endothelial cells fortified with the isolated fraction from native honey enriched in antioxidants, exposed to peroxyl radicals from 1,1-diphenyl-2-picrylhydrazyl (AAPH, 10 mM) and to hydrogen peroxide (H2O2, 50-100 microM), indicated that phenolic acids and flavonoids were the main causes of the protective effect. (researchgate.net)
  • Complement, a complex system of enzymes and regulators endowed with multiple biological activities (opsonisation, chemoattraction of leukocytes, cell lysis, cell activation), is a major component of innate defence against pathogens. (europa.eu)
  • Vascular endothelial cell (EC)-derived factors play an important role in endothelial-cardiomyocyte crosstalk and could save cardiomyocytes (CMs) from injury. (mdpi.com)
  • Lycopene is a powerful antioxidant that might help protect cells from damage. (medlineplus.gov)
  • Certain scavenger receptors can internalize micro-organisms as well as apoptotic cells. (bvsalud.org)
  • Department of Cell Biology, Cleveland Clinic Research Institute, Ohio 44195. (rupress.org)
  • Caroline is a Professor of Vascular Cell Biology and is Head of Comparative Biomedical Sciences. (rvc.ac.uk)
  • Caroline was appointed to a Lectureship position at the Royal Veterinary College in late 1997 and now holds a personal Chair in Vascular Cell Biology. (rvc.ac.uk)
  • Caroline is an internationally recognised expert in vascular cell signalling and biology with 20 years' experience in the field. (rvc.ac.uk)
  • King's College London) fuelled her interest in vascular cellular signalling and her subsequent post-doctoral position with Professor Jeremy Pearson at King's College London (KCL) allowed her to pursue this interest in human endothelial cells. (rvc.ac.uk)
  • In transfected human TLR2 cell lysate, a 90 kDa band is observed. (novusbio.com)
  • TLR2 is differentially expressed in human cells. (novusbio.com)
  • Western Blot: SR-BI Antibody [NB400-104] - SR-BI antibody was tested in human adrenal cell lysate. (novusbio.com)
  • A histological examination of tissue sections, undertaken after the administration of AcAc LDL or Ac LDL (labeled with either 125I or a fluorescent probe) to rats, dogs, or guinea pigs, was used to identify the specific cells binding and internalizing these lipoproteins in vivo. (rupress.org)
  • In vivo killing of primary HIV-infected cells by peripheral-injected early memory-enriched anti-HIV duoCAR T cells. (academictree.org)
  • Endothelial cells (ECs) form a semi-permeable monolayer that separates the bloodstream from underlying tissues and regulate the infiltration of blood cells and proteins through the vessel wall ( 1 , 2 ). (spandidos-publications.com)
  • This has also been reported to work well in bronchial epithelial cells (UBH16E) and THP-1 cells. (novusbio.com)
  • The expression of TLR2 on different cell types are regulated by different immune response modifiers. (novusbio.com)