• ESCs have the characteristic properties of a stem cell: self-renewal and differentiation. (wikipedia.org)
  • Role of insulin-like growth factors in endothelium differentiation ECs derived from stem cells are the beginning of vasculogenesis. (wikipedia.org)
  • It is well established that insulin-like growth factor (IGF) signaling is important for cell responses such as mitogenesis, cell growth, proliferation, angiogenesis, and differentiation. (wikipedia.org)
  • Differentiation is the process by which a cell becomes more specialized. (wikipedia.org)
  • The goal is to characterize the dynamic flow conditions which drive endothelial cell mechanotransduction, cellular growth, and morphological maturation, as well as the differentiation of ADSCs into endothelial-like phenotypes. (aps.org)
  • The team visualized endothelial differentiation step-by-step from the endoderm to the HSPC niche-constituting ECs via islet1+ endothelial progenitors by live imaging-based lineage tracing. (go.jp)
  • As a means to generate sufficient autologous ECs, researchers from the laboratory of Yuanyuan Zhang (Wake Forest School of Medicine, Winston-Salem, NC, USA) recently assessed the possible differentiation of ECs from urine-derived stem cells (USCs), cells originating from the parietal cells in kidney glomeruli that possess robust proliferative abilities, multipotent differentiation capacity, and robust paracrine-acting effects [1-4]. (stemcellsportal.com)
  • now reveal that differentiation of USCs generates ECs with robust endothelial function in vitro and endothelial potential in vivo, thereby suggesting this approach as a non-invasive, simple, and low-cost method [5] to produce sufficient autologous ECs for applications in tissue-engineered vascular regeneration or the repair of endothelial dysfunction [6]. (stemcellsportal.com)
  • Differentiation of USCs in endothelial medium generated EC-like cells by day 14 that displayed endothelial characteristics at the morphological, ultrastructural (tight junction formation), and functional marker expression levels (e.g. (stemcellsportal.com)
  • Multipotential differentiation of human urine-derived stem cells: potential for therapeutic applications in urology. (stemcellsportal.com)
  • Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads loaded with growth factors. (stemcellsportal.com)
  • Human Urine-Derived Stem Cell Differentiation to Endothelial Cells with Barrier Function and Nitric Oxide Production. (stemcellsportal.com)
  • Endothelial differentiation of amniotic fluid-derived stem cells: synergism of biochemical and shear force stimuli. (bakerco.com)
  • 4 In addition, vascular endothelial growth factor (VEGF) is an angiogenic factor that regulates endothelial survival, proliferation, differentiation, and vascular permeability, and has been shown to be an important candidate for cancer therapy as it is upregulated in many tumors. (beckman.com)
  • Remaining obstacles include the high expense of differentiation media, use of animal-derived materials and the need for cell sorting [ 13 , 14 , 15 ]. (biomedcentral.com)
  • In vivo and in vitro experiments demonstrate that not only vascular endothelial cells growth factor (VEGF) enhances EC fate commitment, but also basic fibroblast growth factor (bFGF) further facilitates EC differentiation [ 17 , 18 , 19 ]. (biomedcentral.com)
  • The components of STEMdiff™ Endothelial Expansion Culture Kit are included in STEMdiff™ Endothelial Differentiation Kit (Catalog #08005). (stemcell.com)
  • Promotes embryonic stem cell (ESC) differentiation into cardiomyocytes. (fishersci.com)
  • CCN1 also induced Jag1 expression in hepatic stellate cells, whereupon they interacted with hepatic progenitor cells to promote their differentiation into cholangiocytes. (jci.org)
  • Stem cells can divide into two cells identical to the original stem cell or into differentiated cell types, depending on their origin and differentiation potency. (bvsalud.org)
  • By definition, adult stem cells are capable of differentiation into at least two lineages and have the property of self-renewal. (bvsalud.org)
  • Endothelial cells (EC) derived from embryonic stem cells (ESC) require additional functional characterization before they are used as a cell therapy in order to enhance their potential for engraftment and proliferation. (ca.gov)
  • For several decades, neovascularization is believed to be accomplished by proliferation of mature endothelial cells residing in the local vessels through the process of angiogenesis [ 6 ]. (hindawi.com)
  • Results: Conditioned medium from hemangioma-derived endothelial cells stimulated the proliferation of cultured mast cells. (sacredheart.edu)
  • The same conditioned medium was unable to stimulate proliferation of mast cells expressing a defective receptor for SCF. (sacredheart.edu)
  • VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
  • The Ewing s sarcoma cell line TC71 overexpresses vascular endothelial growth factor isoform 165 (VEGF165), a potent proangiogenic molecule that induces endothelial cell proliferation, migration, and chemotaxis. (conicet.gov.ar)
  • The OVCAR-3 cells, which are sensitive to doxorubicin (DOX), exhibited a preference for biosynthetic pathways associated with cell proliferation. (bvsalud.org)
  • These cells are classified as totipotent and they can form any of the tissue types found in the adult body, in addition to having unlimited proliferation potential 6 . (bvsalud.org)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • After 7 days of mesoderm and endothelial induction, the resulting cells can be expanded for 5 - 6 passages to generate large numbers of highly pure, mature endothelial cells that typically reach ≥ 99% CD144+CD31+ by passage 2 and maintain this phenotype until passage 5. (stemcell.com)
  • In vitro investigation of human umbilical vein endothelial cell (HUVEC) and adipose-derived stem cell (ADSC) responses in a biomimetic 3D bioprinted blood vessel model under complex physiological flows was performed. (aps.org)
  • In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
  • After her graduate studies, she joined Prof Karl Tryggvason's laboratory at Duke-NUS Medical School as a research fellow in 2013 where her main focus has been to culture endothelial cells for therapeutic purposes and developing different cell models for in vitro drug testing. (selectbiosciences.com)
  • To study the migration of microglia across the BBB, we developed an in vitro co-culture system of mouse brain endothelial cells (MBECs) and Ra2 microglia using Transwell inserts. (portlandpress.com)
  • This study examines the possibility of labelling limited numbers of axolotl blastema cells (aBC) and pig endothelial progenitor cells (pEPC) with VSOP and detecting these in vitro and in vivo using a traditional clinical 1.5 T scanner. (au.dk)
  • T1- and T2*-weighted MRI was applied to labelled and control cells in vitro and to cells implanted in live axolotl tail and dead porcine heart, respectively. (au.dk)
  • The resulting VEGF165-deficient tumor cells had normal growth rates in vitro, but had delayed growth when implanted into mice. (conicet.gov.ar)
  • The direct effects of CNT were evaluated in vitro in human aortic endothelial cells (HAEC). (cdc.gov)
  • ESCs will eventually produce endothelial cells (ECs), which create the thin-walled endothelium that lines the inner surface of blood vessels and lymphatic vessels. (wikipedia.org)
  • Endothelial cells can be found throughout the whole vascular system and they also play a vital role in the movement of white blood cells ECs were first thought to arise from extraembryonic tissues because blood vessels were observed in the avian and mammalian embryos. (wikipedia.org)
  • When vasculogenesis occurs the cells transform into different versions throughout the process to eventually become the earliest blood vessels. (wikipedia.org)
  • Endothelial cells (ECs) line blood vessels and can serve as specialized vascular niches for hematopoietic stem and progenitor cells (HSPCs), a special environment where HSPCs reside and self-renew. (go.jp)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • Endothelial cells (ECs) form the endothelium, a layer of single-cell thickness that coats the interior wall of all blood vessels including arteries, capillaries, and veins. (beckman.com)
  • Endothelial cells form a semi-permeable barrier between the blood and surrounding tissues within all blood vessels in the body. (beckman.com)
  • Endothelial cells (ECs) form the inner lining of all blood vessels of the body play important roles in vascular tone regulation, hormone secretion, anticoagulation, regulation of blood cell adhesion and immune cell extravasation. (biomedcentral.com)
  • Endothelial cells form the inner lining of all blood vessels of the mammalian organism. (biomedcentral.com)
  • Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
  • Hematopoietic stem cells emerge from endothelial cells in blood vessels during embryogenesis through the endothelial to hematopoietic transition. (biorxiv.org)
  • While tissue engineers have found dozens of ways to coax stems cells into forming specific kinds of cells and tissues, they still cannot grow tissues with vasculature -- capillaries and the larger blood vessels that can supply the tissues with life-giving blood. (sciencedaily.com)
  • Each of the trillions of living cells in the human body are constantly supplied with oxygen and nutrients by tiny blood vessels known as capillaries. (sciencedaily.com)
  • The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
  • They found that islet1 -expressing cells are the progenitors of the venous ECs that constitute the majority of the HSPC niche (Figure 1A). (go.jp)
  • The present results clearly demonstrate that ECs originating from the endoderm via islet1 + endothelial progenitors play a specialized role in forming a functional vascular HSPC niche (Figure 1C). (go.jp)
  • C) We have identified that islet1 + endothelial progenitors originate from endoderm and finally differentiate into posterior venous ECs in a process dependent on Bmp-Smad signaling, requiring Npas4l-Etsrp function. (go.jp)
  • Medina RJ , Barber CL , Sabatier F , Endothelial progenitors: a consensus statement on nomenclature . (avma.org)
  • It is as yet unresolved whether these first HSCs are derived from intraembryonic hemangioblasts, hemogenic endothelial cells, or other progenitors. (ox.ac.uk)
  • During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
  • abstract = "Mesenchymal stem cells (MSCs) have anti-inflammatory and immunosuppressive properties and may be useful in the therapy of diseases such as arteriosclerosis. (birmingham.ac.uk)
  • Important signaling factors for vasculogenesis are TGF-β, BMP4, and VEGF, all of which promote pluripotent stem cells to differentiate into mesoderm, endothelial progenitor cells, and then into mature endothelium. (wikipedia.org)
  • Not only is VEGF critical for mesoderm cells to become an EC, but also for EPCs to differentiate into mature endothelium. (wikipedia.org)
  • Mesenchymal stem cells (MSCs) are multipotent stem/progenitor cells which can differentiate to several mesodermal derivatives and possess an ability to secrete factors involved in neovascularization and immunomodulation [ 1 , 2 ]. (hindawi.com)
  • HSPCs are multipotent cells that can self-renew and differentiate to give rise to all blood cell lineages. (go.jp)
  • Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • CD341 bone marrow stem cells can differentiate into endothelial and hematopoietic cells. (conicet.gov.ar)
  • The key to these cells' utility is their ability to differentiate into many different cell types depending on the stimulus received and they have been used in treatments for diseases such as cancer and neural degeneration, in rehabilitation of tetraplegic and paraplegic patients and even in dentistry 1 . (bvsalud.org)
  • Hematopoietic stem cells are cells isolated from blood and bone marrow that can differentiate into a variety of different specialized cells and suffer apoptosis 1 . (bvsalud.org)
  • Mice deficient in eNOS (Nos3(-/-)) show reduced vascular endothelial growth factor (VEGF)-induced mobilization of endothelial progenitor cells (EPCs) and increased mortality after myelosuppression. (nih.gov)
  • Mesodermal progenitor cells are further differentiated into ECs by utilizing a combination of human vascular endothelial growth factor A165 (VEGF), basic fibroblast growth factor (bFGF), 8-Bromoadenosine 3′,5′-cyclic monophosphate sodium salt monohydrate (8Bro) and melatonin (Mel) for 48 h. (biomedcentral.com)
  • Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
  • We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
  • Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)
  • We studied the secretome profile of gestational tissue-derived MSCs and their effects on functions of endothelial progenitor cells (EPCs), another angiogenic cell type that plays an important role during the neovascularization. (hindawi.com)
  • However, recent studies have demonstrated that other angiogenic cells called endothelial progenitor cells (EPCs) also play an important role during the formation of new vessels through the process of vasculogenesis [ 7 - 9 ]. (hindawi.com)
  • BALB/c mice were treated with total body irradiation (TBI) and then infused with C57Bl6-derived endothelial progenitor cells (EPCs) to augment endogenous BM EC activity. (duke.edu)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
  • Endothelial cells (EC) derived from embryonic stem cells (ESC) require additional functional characterization before they are used as a cell therapy. (ca.gov)
  • Overall, the authors provide substantial evidence that non-invasively collected USCs represent an exciting new source of autologous ECs for applications in tissue-engineered vascular regeneration or repair of endothelial dysfunction. (stemcellsportal.com)
  • Vitamin C Improves Endothelial Dysfunction of Epicardial Coronary Arteries in Hypertensive Patients. (drcalapai.com)
  • These findings suggest that increased oxidative stress contributes to endothelial dysfunction in hypertensive patients. (drcalapai.com)
  • We further characterized the subphenotype of our ESC-EC and observed both venous and arterial markers on individual cells with a larger percentage of the cells exhibiting a venous phenotype. (ca.gov)
  • The clinical-grade cell line RC11 was used to generate hESC-ECP which were identified as mostly endothelial (60% CD31+/CD144+), with the remainder of the subset expressing various pericyte/mesenchymal stem cell markers. (ed.ac.uk)
  • Understanding how cancer stem cells affect tumor heterogeneity, the contribution of resident stem and progenitor cells to the tumor niche, and the search for true cancer stem cell markers are other examples of scientific challenges in this fast-growing research area. (hindawi.com)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • 2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)
  • The ESC-EC produced less NO on a per cell basis, but the same amount of NO if quantified based on the area of endothelial tissue. (ca.gov)
  • Previous studies demonstrated that MSCs could ameliorate the pathology associated with ischemic heart disease, ischemic stroke, and peripheral vascular disease by releasing several critical factors that increase cell survival, provide an appropriate microenvironment for repairing damaged tissue, and induce neovascularization [ 3 - 5 ]. (hindawi.com)
  • They also serve as specialized vascular niches that provide instructive signals to tissue-specific stem cells or parenchymal cells for tissue formation and repair. (go.jp)
  • Urine derived cells are a potential source for urological tissue reconstruction. (stemcellsportal.com)
  • Primordial endothelial cells specialize into tissue-specific phenotypes of arterial, venous, hemogenic, and lymphatic subtypes with distinct functions for vascular development. (beckman.com)
  • These tumors also contain large numbers of mast cells, compared with the normal surrounding skin or tissue. (sacredheart.edu)
  • Conclusions: Our data suggest that increased release of SCF is a property of hemangioma-derived endothelial cells that may account for the high numbers of mast cells observed in hemangioma tissue. (sacredheart.edu)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • Implanted cells were easily distinguishable as dark spots with negative contrast relative to surrounding tissue. (au.dk)
  • Sandström J., Eggermann E., Charvet I., Roux A., Toni N ., Greggio C., Broyer A., Monnet-Tschudi F., Stoppini L. "Development and characterization of a human embryonic stem cell-derived 3D neural tissue model for neurotoxicity testing. (chuv.ch)
  • Tissue engineering, also known as regenerative medicine, is a field aimed at integrating advances in stem cell biology and materials science to grow transplantable replacement tissues and organs. (sciencedaily.com)
  • Adult stem cells are classified as pluripotent and are undifferentiated cells that remain quiescent in tissues until stimulated, when they can create cell types that are compatible with the tissue in which they reside. (bvsalud.org)
  • They are multipotent, which describes the ability to give rise to many cell types, whereas a pluripotent stem cell can give rise to all types. (wikipedia.org)
  • Pluripotent stem cell-derived differentiated endothelial cells offer high potential in regenerative medicine in the cardiovascular system. (ed.ac.uk)
  • Efficiently expand human pluripotent stem cell (hPSC)-derived endothelial cells using xeno-free STEMdiff™ Endothelial Expansion Culture Kit (Catalog #100-1218). (stemcell.com)
  • Hematopoietic stem cell transplantation-associated thrombotic microangiopathy (HSCT-TMA) is a life-threatening syndrome that occurs in adult and pediatric patients after hematopoietic stem cell transplantation. (biomedcentral.com)
  • Hematopoietic stem cell transplantation and associated risk factors precipitate endothelial injury, leading to HSCT-TMA and other endothelial injury syndromes such as hepatic veno-occlusive disease/sinusoidal obstruction syndrome, idiopathic pneumonia syndrome, diffuse alveolar hemorrhage, capillary leak syndrome, and graft-versus-host disease. (biomedcentral.com)
  • The transplantation of these cells is one of the most common treatments to cure patient suffering of blood diseases. (biorxiv.org)
  • Rare variants in complement system genes associate with endothelial damage after pediatric allogeneic hematopoietic stem cell transplantation. (cdc.gov)
  • Scholars@Duke publication: Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo. (duke.edu)
  • In this treatment, we identified that CD44 is up-regulated under the hypoxia and this molecule supports stemness of cancer stem cells and induces drug resistance of cancer cells. (nii.ac.jp)
  • Cells labelled at relatively high concentrations appear dark with less contrast, whereas labelling at low concentrations induces positive contrast. (au.dk)
  • Melero-Martin JM , Khan ZA , Picard A , In vivo vasculogenic potential of human blood-derived endothelial progenitor cells . (avma.org)
  • 2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
  • In vivo, cells labelled at low concentration appeared bright with positive contrast, whereas cells labelled at higher concentration again appeared dark. (au.dk)
  • Capillary hemangiomas, the most common tumors in young children, consist of proliferating capillary vessels and endothelial cells. (sacredheart.edu)
  • Immunohistochemical analysis revealed decreased infiltration of CD341 cells into both VEGF165-deficient tumors. (conicet.gov.ar)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • This week, Nature raises important questions about one company that works with adult stem cells: Celltex Therapeutics in Houston, Texas. (nature.com)
  • Nature 's investigation, reported on page 13 , suggests that the company has supplied adult stem cells to Texas doctors who offer unproven treatments to patients, and that the company is involved in these treatments. (nature.com)
  • The US Food and Drug Administration (FDA) considers it to be a crime to inject unapproved adult stem cells into patients. (nature.com)
  • If the Texas Medical Board were to act according to its stated pledge to protect patients, then it would make clear the need for clinical validation of adult stem cells before use and would rescind the medical licences of any doctors in breach of rules on using unapproved treatments. (nature.com)
  • More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
  • Cell tracking using MRI, PET and qPCR in a murine model of limb ischemia demonstrated that hESC-ECP were detectable up to day 7 following injection. (ed.ac.uk)
  • We have recently shown that stem cell factor (SCF), the gene product of the murine steel locus, can act as a chemoattractant for mast cells. (sacredheart.edu)
  • Experimental Design: Cultured endothelial cells derived from a murine hemangioma were compared with normal vascular endothelial cells for the ability to produce and release SCF, a mitogen for mast cells. (sacredheart.edu)
  • This kit includes xeno-free STEMdiff™ Endothelial Expansion Basal Medium and 5X Supplement, and Animal Component-Free Cell Attachment Substrate, and is optimized for the expansion of hPSC-derived endothelial cells and for the maintenance of endothelial marker expression. (stemcell.com)
  • Vasculogenesis is new production of a vascular network from mesodermal progenitor cells. (wikipedia.org)
  • It is important to talk more about vasculogenesis because this is what makes ECs different from other types of cells that are found in the body. (wikipedia.org)
  • Since these cells come from the mesoderm, it can become a wide variety of different things found in many different parts of the body. (wikipedia.org)
  • These islet1 -expressing cells surprisingly originate not from the mesoderm, the germ layer classically defined to give rise to ECs, but from the endoderm. (go.jp)
  • Therapeutic potentials of mesenchymal stem cells (MSCs) depend largely on their ability to secrete cytokines or factors that modulate immune response, enhance cell survival, and induce neovascularization in the target tissues. (hindawi.com)
  • Here we discuss evidence for the role of lectin pathway activation in endothelial injury-associated complications of HSCT and how targeting complement activity may provide therapeutic benefit for patients with HSCT-TMA. (biomedcentral.com)
  • Our work has important therapeutic implications because we demonstrate utilization of human cells and the ability to live-monitor their tubulogenesis potential as they form primitive vessel networks," said study lead author Gisele Calderon, a graduate student in Miller's Physiologic Systems Engineering and Advanced Materials Laboratory. (sciencedaily.com)
  • Ultimately, we'd like to 3-D print with living cells, a process known as 3-D bioprinting, to create fully vascularized tissues for therapeutic applications," said Miller, assistant professor of bioengineering. (sciencedaily.com)
  • In conclusion, we offer evidence that microglia migration across the brain endothelial cell monolayer is increased in the presence of ATP in a manner that involves MMP secretion. (portlandpress.com)
  • We also examined the role of VEGF165 in CD341 cell migration. (conicet.gov.ar)
  • Role of DARPP-32 in Breast Cancer Cell Signalling and Migration. (lu.se)
  • Involvement of eicosanoid signalling in epithelial cell migration. (lu.se)
  • The interplay between stem cell and tumor biology offers an exceptional opportunity to advance our knowledge about cancer, one of the leading causes of death worldwide, with potential impacts in cancer diagnosis and therapy. (hindawi.com)
  • Dr. Nguyen (pronounced as "Gwen") earned a bachelor degree in Biochemistry and Cell Biology at the University of California, San Diego, and a PhD in Integrated Biology and Disease at the University of Southern California, Los Angeles. (selectbiosciences.com)
  • Little is known about molecular mecha- desmosomal glycoproteins, resulting in the nisms affecting mast cell and T lympho- loss of keratinocyte cell-cell adhesion [ 1 ]. (who.int)
  • Common signs and symptoms may stem from decreased hepatic synthetic function (eg, coagulopathy), portal hypertension (eg, variceal bleeding), or decreased detoxification capabilities of the liver (eg, hepatic encephalopathy). (medscape.com)
  • This monolayer of cells acts as a barrier to separate the vascular lumen from surrounding tissues and regulates the passage of fluid and nutrients between the two. (beckman.com)
  • This monolayer of cells is an important endocrine organ, and has been shown to play a major role in regulating blood fluidity, the transfer of solutes and macromolecules, immune responses and inflammation. (beckman.com)
  • Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
  • The addition of microglia or ATP led to the disruption of the MBEC monolayer and significantly decreased barrier function as measured by trans-endothelial electrical resistance (TEER) and electric cell-substrate impedance sensing (ECIS). (portlandpress.com)
  • In a paper published online in the journal Biomaterials Science , a team from the laboratories of Rice bioengineer Jordan Miller and Baylor College of Medicine biophysicist Mary Dickinson showed how to use a combination of human endothelial cells and mesenchymal stem cells to initiate a process called tubulogenesis that is crucial to the formation of blood-transporting capillaries. (sciencedaily.com)
  • Measuring just a few thousandths of a millimeter in diameter, some capillaries are so narrow that individual blood cells must squeeze through them in single-file. (sciencedaily.com)
  • Capillaries are made entirely from networks of endothelial cells, the type of cell that lines the inner surface of every blood vessel in the human body. (sciencedaily.com)
  • In the process of tubulogenesis -- the first step to making capillaries -- endothelial cells undergo a series of changes. (sciencedaily.com)
  • First, they form small, empty chambers called vacuoles, and then they connect with neighboring cells, linking the vacuoles together to form endothelial-lined tubes that can eventually become capillaries. (sciencedaily.com)
  • Cylindrical vessel scaffolds were 3D printed using polylactic acid (PLA) material and the vessel lumen were inoculated with a co-culture of HUVEC and ADSC in a custom-built bioreactor system using cell growth medium ((DMEM, Corning®) and (ECM, Cell Applications Inc)). The vessels were installed in a sterile experimental flow loop facility capable of providing physiological flow forcing conditions. (aps.org)
  • The tumor sections expressed both human VE-cadherin and mouse CD31, indicating involvement of donor-derived human cells in the tumor vessels. (conicet.gov.ar)
  • Immunohistology of aorta-gonads-mesonephros (AGM) regions show that GFP(+) cells are specifically localized to the endothelial layer lining the wall of the dorsal aorta but not to the mesenchyme, strongly suggesting that HSC activity arises within a few cells within the endothelium of the major vasculature. (ox.ac.uk)
  • We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
  • Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)
  • The emergence of the first adult hematopoietic stem cells (HSCs) during mammalian ontogeny has been under intense investigation. (ox.ac.uk)
  • Hematopoietic stem cells (HSCs) emerge during development from the vascular wall of the main embryonic arteries. (lu.se)
  • Endothelial injury can trigger activation of the complement system, promoting inflammation and the development of endothelial injury syndromes, ultimately leading to organ damage and failure. (biomedcentral.com)
  • Fetal haemoglobin (HbF) induction is the primary treatment response, along with improved anaemia, reduced haemolysis, myelosuppression and decreased endothelial inflammation. (cdc.gov)
  • Functional characterization of embryonic stem cell-derived endothelial cells. (ca.gov)
  • Gebara E., Bonaguidi M., Beckervordersandforth R., Sultan S., Udry F., Gijs P., Lie C., Ming G., Song H., Toni N . "Morpho-functional characterization of adult neural hippocampal stem cells reveals two morphotypes of radial glia-like cells. (chuv.ch)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • What barriers exist to the clinical application of USC-derived cells? (stemcellsportal.com)
  • With the aim of translating the use of a human stem cell-derived endothelial cell product (hESC-ECP) for treatment of critical limb ischemia (CLI) in man, we report a GMP-compatible protocol and detailed cell tracking and efficacy data in multiple pre-clinical models. (ed.ac.uk)
  • One doctor claims that the treatments are part of a clinical study run by Celltex and that the company pays him US$500 a time to inject the cells into patients, who are charged up to $25,000 for a course. (nature.com)
  • But he too would not answer Nature 's questions on whether the company knew about the unapproved clinical use of its stem cells or whether he considered such activity to be ethical and legal. (nature.com)
  • How can Celltex propose clinical trials for stem-cell treatments while at the same time it is, according to a doctor involved, paying physicians to use those treatments - or supplying cells to doctors who would no doubt use them - in the clinic? (nature.com)
  • In its discussions, the state's medical board revisited the well-trodden ground of whether adult-stem-cell procedures - in which cells are taken from a patient, processed and cultured, before being reintroduced - should be under FDA jurisdiction, or whether they are akin to simple skin grafts from one part of the body to another, which do not require validation in an FDA-approved clinical trial. (nature.com)
  • These conditions are not discrete diseases, but different clinical manifestations stemming from endothelial injury. (biomedcentral.com)
  • In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
  • The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
  • This review describes current considerations in the use of hydroxyurea for the management of sickle cell disease in the context of clinical severity. (cdc.gov)
  • Here, we used single-cell transcriptomics analysis to compare embryonic and post-natal endothelial cells to investigate the potential of adult vasculature to be reprogrammed in hematopoietic stem cells. (biorxiv.org)
  • As a result, when tumor vasculature was focused, we found that cancer stem cells existing near myofibroblasts abundantly localizing at perivascular area show drug resistance. (nii.ac.jp)
  • Sanchez-Pascual I., O'Laoi R., El M'Ghari I., Kocher-Braissant J., Ellisman MH, Toni N . "Fine processes of Nestin-GFP positive radial glia-like stem cells in the adult dentate gyrus ensheathe local synapses and vasculature. (chuv.ch)
  • But the fragility of endothelial cells during laboratory growth has limited the utilization of this critical cell type, which is found in all vasculature. (sciencedaily.com)