Endogenous gene promoter activity chloramphenicol. Medical search. Frequent questions

Commonly used reporter genes that induce visually identifiable characteristics usually involve fluorescent and luminescent proteins. (wikipedia.org)
Over the next 100 years, many significant discoveries lead to the conclusions that genes encode proteins and reside on chromosomes, which are composed of DNA. (addgene.org)
The biotinylated proteins were affinity-purified for mass spectrometric analysis, and the candidate GRA proteins from BioID data set were identified by gene tagging. (biomedcentral.com)
Using NcGRA17-BirA* fusion protein as bait, we have identified some known GRAs and verified localization of 11 novel GRA proteins by gene endogenous tagging or overexpression in the Nc1 strain. (biomedcentral.com)
These proteins are intracellular transcription factors that directly regulate gene expression in response to lipophilic molecules. (indigobiosciences.com)
Conclusion】 The expression trends of ADAMTS 1, PI 3 K and A k t genes and proteins were consistent and showed a positive correlation in ovary and granulosa cells of Mongolian sheep,suggested that ADAMTS 1 gene played an important role in twin birth of Mongolian sheep mediated by PI3K/Akt signaling pathway. (chvm.net)

Of note, we observed that MAPCAs differentially express several genes at much higher levels than either PA or aorta.MAPCAs differ from PA or aorta by significantly altered levels in gene expression, suggesting a transcriptional basis for their physiology that will guide a further understanding of the pathobiology of MAPCAs and TOF. (stanford.edu)
Transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases PRMT1 and PRMT4. (embl.de)
Inside the nucleus, HER family members (EGFR, HER2, and HER3) have been shown to function as co-transcriptional activators for various cancer-promoting genes. (plos.org)
To understand how the B 1 and B 2 regions influenced the transcriptional functions of nuclear HER3, we performed cyclin D1 promoter-luciferase assays in which HER3 deleted of the B 1 and B 2 regions was severely hindered in regulating this promoter. (plos.org)
The reactivation led to transcriptional initiation at the authentic cap sites of the late E2A promoter and was not associated with promoter demethylation, at least not in both DNA complements. (uni-bielefeld.de)
The methylated promoter was not demethylated, at least not in both complements, and it was shown that reactivation of the methylated promoter entailed transcriptional initiation at the authentic late E2A cap site. (uni-bielefeld.de)
All the previously identified E. coli FabR transcriptional target genes ( fabA , fabB and yqfA ) were shown to be direct S. Typhimurium FabR targets as well. (biomedcentral.com)

The combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can be used to study DNA fragments of interest, such as genes. (addgene.org)
Plasmids that are used most commonly in the field of recombinant DNA technology have been optimized for their use of studying and manipulating genes. (addgene.org)
The present invention relates to promoter DNAs in the presence of organic acids, DNA constructs, transformants, expression methods of a recombinant gene, and their uses in organic acid production methods. (justia.com)
With advances in recombinant DNA technology, techniques to obtain objective gene products have developed through expression of a foreign gene in hosts such as bacteria, fungi, animals, plants, and insects, and proliferation of the transformants. (justia.com)
For example, culture of recombinant yeasts allows production of large amounts of objective gene products by means of fermentative production. (justia.com)
Although such a stable high-level gene expression system has been established, a promoter with strong expression potency is needed to further increase the productivity of recombinant products. (justia.com)
Thus, an object of the invention is to provide promoters usable in the presence of an organic acid, DNA constructs having the promoters, transformants comprising the DNA constructs, expression methods of recombinant genes in the transformants, and production methods of organic acids using the transformants. (justia.com)
Although attractive fermentation performances were reached, significant improvements are required to eliminate recombinant plasmids, antibiotic resistances and inducible promoters, and increase ethanol tolerance. (biomedcentral.com)
Here, we report a new strain with chromosomally integrated ethanologenic pathway under the control of a constitutive promoter, without recombinant plasmids and resistance genes. (biomedcentral.com)

Transient transfection of promoter-chloramphenicol O-acetyltransferase reporter constructs into primary human connective tissue fibroblasts shows that a 904 bp fragment that hybridizes to a murine TIMP-1 promoter fragment contains a functional promoter. (uea.ac.uk)
Many methods of transfection and transformation - two ways of expressing a foreign or modified gene in an organism - are effective in only a small percentage of a population subjected to the techniques. (wikipedia.org)

The yeast promoters published include alcohol dehydrogenase 1 gene (ADH1) promoter (J Ferment Bioeng, 1998, Vol. 8 6(3) pp 284-289), triose phosphate isomerase gene (TPI1) promoter (FEMS Microbial Lett, 1999, Feb. Vol. 171 (2) pp 133-140), and the like. (justia.com)

Use to confirm the function of the products of genes or cDNAs that are thought to encode a phytoene synthase enzyme, or to screen genomic or cDNA libraries for plasmids containing such genes (crtB) or cDNAs (psy). (addgene.org)
Plasmids are fragments of double-stranded DNA that typically carry genes and can replicate independently from chromosomal DNA. (addgene.org)
Typically plasmids contain the minimum essential DNA sequences for this purpose, which includes a DNA replication origin, an antibiotic-resistance gene, and a region in which exogenous DNA fragments can be inserted. (addgene.org)

Constructs of -738/+95 to -194/+21 are inducible with serum or phorbol ester to a similar extent to the endogenous TIMP-1 gene. (uea.ac.uk)
Many of the NRs act as ligand-inducible transcription factors, responding to endogenous and exogenous chemicals. (indigobiosciences.com)

The inventors obtained mRNA from lactic acid-producing yeasts or yeasts growing in lactic acid-containing medium and conducted thorough gene expression analysis by a quantitative PCR method. (justia.com)
A high frequency of BCL2 mRNA over expression plus a reasonably minimal frequency of BAX mRNA above expression detected in each analyzed leukemia within this research, indicate that altered transcription of those genes may very well be involved in leukemogenesis. (microrna21.com)
Evaluated the mRNA gene expression profile of 12 HDAC genes by quantitative serious time polymerase chain reaction in 94 consecutive childhood acute lymphoblastic leukaemia samples and its association with clinical biological functions and survival. (microrna21.com)
Putative in vivo CMV promoter-derived transcript produced from bicistronic plasmid pbetaGAL/5'(-162)/CAT which comprises beta galactosidase and chloramphenicol acetyl transferase as the first and the second cistron, respectively and the part of human XIAP mRNA (from nt -162 to nt -1 of the original sequence) mRNA. (iresite.org)
Part of the 5' UTR from human XIAP mRNA (from nt -162 to nt -1 of the original sequence) has been cloned between the beta galactosidase and chloramphenicol acetyl transferase reporter genes. (iresite.org)

Examples include the gene that encodes jellyfish green fluorescent protein (GFP), which causes cells that express it to glow green under blue light, the enzyme luciferase, which catalyzes a reaction with luciferin to produce light, and the red fluorescent protein from the gene dsRed [fr]. (wikipedia.org)
A common reporter in bacteria is the E. coli lacZ gene, which encodes the protein beta-galactosidase. (wikipedia.org)
Reporter genes can produce a protein that has little obvious or immediate effect on the cell culture or organism. (wikipedia.org)
Reporter gene assay have been increasingly used in high throughput screening (HTS) to identify small molecule inhibitors and activators of protein targets and pathways for drug discovery and chemical biology. (wikipedia.org)
EcN was armed with HlyE, a small cytotoxic protein, under the control of the araBAD promoter (P BAD ). (nature.com)
We describe the residue-specific methylation of E2F-1 by the asymmetric dimethylating protein arginine methyltransferase 1 (PRMT1) and symmetric dimethylating PRMT5 and relate the marks to different functional consequences of E2F-1 activity. (embl.de)
The Tudor domain protein p100-TSN reads the symmetric methylation mark, and binding of p100-TSN downregulates E2F-1 apoptotic activity. (embl.de)
The assembly of PRMT6 protein network allowed us to formulate functional hypotheses which led to the discovery of new molecular partners for the architectural transcription factor HMGA1a, a known substrate for PRMT6, and to provide evidences for a modulatory role of HMGA1a on the methyltransferase activity of PRMT6. (embl.de)
In contrast, the Fushi Tarazu homeodomain protein is an activator, both in cultured cells and in Drosophila embryos, where it activates several known target genes, including its own gene. (biologists.com)
The promoter includes DNA with promoter activity of high osmolarity response 7 gene (HOR7 gene), glycelaldehyde 3 phosphate dehydrogenase 2 gene (TDH2 gene), heat shock protein 30 gene (HSP30), hexose transport protein 7 gene (HXT7 gene), thioredoxin peroxidase 1 gene (AHP1 gene), or membrane protein 1 associated gene gene) of yeasts. (justia.com)
The inventors have already established a system to express an L-lactate dehydrogenase gene using an original promoter function of the foreign PDC1 gene and exclude the expression of PDC1 protein that is expressed by the original foreign promoter, by linking the objective valuable gene (L-lactate dehydrogenase gene in this case) in the downstream of chromosomal PDC1 promoter and disrupting the yeast chromosomal PDC1 gene simultaneously. (justia.com)
Therefore, such a promoter can be used to increase the production of organic acids by manipulatively associating protein genes involved in organic acid production. (justia.com)
FASLG, the protein encoded by this gene would be the ligand for FAS. (microrna21.com)
These findings implicated the E1A 289 amino acid residue protein of Ad2, a well-known transactivator, as the reactivating function of the endogenous, previously dormant, late E2A promoter-CAT gene assembly. (uni-bielefeld.de)
More specifically, the present invention concerns altering tolerance in plants to various environmental stresses and to increasing seed yield by modifying plant haemoglobin gene expression and/or by modifying plant haemoglobin protein levels. (allindianpatents.com)
Objective】 This study was aimed to clone the Toll-like receptor 4 (TLR4) gene in Cygnus olor and analyze its encoded protein by bioinformatics,so as to provide an important reference basis for further exploring the immune mechanism of TLR4 protein against pathogenic microorganisms in Cygnus olor . (chvm.net)
Method】 The sequence of TLR 4 gene CDS in Cygnus olor was amplified by PCR and cloned.NCBI-BLAST and Mega-X were used for sequence comparison and phylogenetic tree construction.The structure and function of TLR4 protein and its interaction with host protein were predicted using ProtParam,SWISS-MODEL,etc. (chvm.net)
Conclusion】 TLR4 gene in Cygnus olor was closely related to Anser anser and Anser cygnoides ,with obvious codon species selectivity,and contained 6 consecutive rare codons.TLR4 protein had transmembrane domains and signal peptides,and was closely related to innate immune response,signal transduction and stress response. (chvm.net)
In addition, endogenous activity of GluK1 subunit containing KARs in the immature hippocampus maintains high excitability of interneurons, typical for early development, by regulating the function of calcium-activated potassium channels (SK channels) in a G-protein dependent manner [ 42 ]. (biomedcentral.com)

For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. (addgene.org)
A significant public health concern observed in this study is that multi drug resistant: commensal E. Coli strains may constitute a potential reservoir of resistance genes that could be transferred to pathogenic bacteria. (researchcub.info)

In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants. (wikipedia.org)
This enzyme causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal. (wikipedia.org)
An example of a selectable marker which is also a reporter in bacteria is the chloramphenicol acetyltransferase (CAT) gene, which confers resistance to the antibiotic chloramphenicol. (wikipedia.org)
In the case of selectable-marker reporters such as CAT, the transfected population of bacteria can be grown on a substrate that contains chloramphenicol. (wikipedia.org)
The intrinsic limitation of P BAD for the gene expression is known to be negated by glucose and afflicted with all-or-nothing induction in host bacteria. (nature.com)
It is naturally produced in bacteria and displays a pore-forming activity that lyses mammalian erythrocytes 16 . (nature.com)
Although they can be found in archaea and eukaryotes, they play the most significant biological role in bacteria where they can be passed from one bacterium to another by a type of horizontal gene transfer (conjugation), usually providing a benefit to the host, such as antibiotic resistance. (addgene.org)
Antibiotic usage selects for resistance not only in pathogenic bacteria but also in the endogenous flora of exposed individuals (animals and humans). (researchcub.info)

A number of these targets were confirmed by gene overexpression or endogenous tagging in the Nc1 strain, thereby validating our approach. (biomedcentral.com)

Reporter genes are often used as an indication of whether a certain gene has been taken up by or expressed in the cell or organism population. (wikipedia.org)
To introduce a reporter gene into an organism, scientists place the reporter gene and the gene of interest in the same DNA construct to be inserted into the cell or organism. (wikipedia.org)
It is important to use a reporter gene that is not natively expressed in the cell or organism under study, since the expression of the reporter is being used as a marker for successful uptake of the gene of interest. (wikipedia.org)
the reporter gene can be expressed constitutively (that is, it is "always on") or inducibly with an external intervention such as the introduction of Isopropyl β-D-1-thiogalactopyranoside (IPTG) in the β-galactosidase system. (wikipedia.org)
As a result, the reporter gene's expression is independent of the gene of interest's expression, which is an advantage when the gene of interest is only expressed under certain specific conditions or in tissues that are difficult to access. (wikipedia.org)
Reporter genes can be used to assay for the expression of a gene of interest that is normally difficult to quantitatively assay. (wikipedia.org)
They are ideally not present in the native genome to be able to isolate reporter gene expression as a result of the gene of interest's expression. (wikipedia.org)
To activate reporter genes, they can be expressed constitutively, where they are directly attached to the gene of interest to create a gene fusion. (wikipedia.org)
In building the DNA construct, a segment of DNA coding for a flexible polypeptide linker region is usually included so that the reporter and the gene product will only minimally interfere with one another. (wikipedia.org)
Reporter genes can also be expressed by induction during growth. (wikipedia.org)
In these cases, trans-acting elements, such as transcription factors are used to express the reporter gene. (wikipedia.org)
Ectopic expression of PML recruits HDAC7 to PML NBs and leads to activation of MEF2 reporter activity. (microrna21.com)

Then, to disrupt the PDC1 gene on a yeast chromosome, an expression cassette having a L-lactate dehydrogenase gene in the downstream of these promoters was integrated into the PDC1 gene locus that had previously been mapped to the chromosome, and the production of large amounts of L-lactic acids was confirmed. (justia.com)
During thymocyte maturation, enhancers of genes encoding for TCRδ ( Tcrd ) and TCRα ( Tcra ), Eδ 8 , and Eα, work as a developmental switch controlling transition from Tcrd to Tcra activity at the Tcrad locus. (aai.org)
3xFLAG tag inserted into the endogenous rpb-2 locus. (umn.edu)

Transposon vectors can be used in the frog for transgenesis and for insertional mutagenesis where enhancer trap and gene trap constructs are used to identify genomic loci involved in developmental processes. (biomedcentral.com)

Lately research connected to mechanisms of leukemia cell resistance to Fas FasL mediated apoptosis selleck chemical such as Fas and FasL mutation and expression abnormality, Fas signaling transduction pathway abnormality, and regulatory have an impact on of apoptotic regulatory genes on Fas FasL process, as well as tactics replying to antiapoptosis of leukemia cells including NF kappa B, XIAP, membrane receptor CD28 and matrix metalloproteinase 7 obtained some pro gresses. (microrna21.com)

In contrast to these three Pols, which under certain conditions impede growth of the dnaN159 strain ( 29 , 39 , 40 ), presumably by impairing DNA replication, the catalytic DNA polymerase activity of Pol I ( polA ) is essential for viability of the dnaN159 strain ( 38 ). (asm.org)

We have isolated a genomic clone of the human TIMP-1 gene. (uea.ac.uk)
Depending on the organism and availability, mt-tRNA Val or mt-tRNA Phe are incorporated, most likely due to their genomic proximity to mt-rRNA genes and consequent near stoichiometric presence of their transcripts 4 , 8 . (nature.com)
Thanks to rapidly growing genomic and metagenomic sequencing data, high-throughput prediction approaches based on amino acid sequence similarity have emerged to efficiently screen potential TS genes [ 16 ]. (biomedcentral.com)

AV coaptation, whether phasic or chronic, preserved phenotypic gene expression. (stanford.edu)

The modification of the three 5' CCGG 3' sequences at nucleotides +24, +6 and -215, relative to the cap site in this promoter, sufficed to silence the gene in transient expression either in Xenopus laevis oocytes or in mammalian cells, and after the fixation of the E2A promoter-chloramphenicol-acetyltransferase (CAT) gene construct in the genome of hamster cells. (uni-bielefeld.de)
It was demonstrated in transient expression experiments that the 5' CCGG 3'-methylated late E2A promoter was at least partly reactivated in cell lines constitutively expressing the E1 region of Ad2 or of adenovirus type 5 (Ad5) DNA. (uni-bielefeld.de)

Methods: To analyze the impact of having gastrointestinal problems, among other socioeconomic conditions, on the onset of depression and anxiety in the Spanish population, we compare treating gastrointestinal diseases as exogenous in a single-equation probit model with a bivariate probit model in which this variable is treated as endogenous. (unican.es)

A subset of highly regulated genes was validated using quantitative PCR. (stanford.edu)

Presynaptic regions (Zone 2 and its translation is activated by the Prab-3 promoter is expressed panneuronally to induce expression of F-actin required for germline development in Drosophila. (sophieluis.com)

Antioxidant genes such as ferritin are transcriptionally activated in oxidative stress via the antioxidant responsive element (ARE), to which nuclear factor-E2-related factor 2 (Nrf2) binds and activates transcription. (embl.de)
Thus, the promoter that activates the transcription of operatively-associated DNA in the presence of an organic acid is useful to increase the objective gene product in the presence of an organic acid, in particular, those are useful to increase the objective product during the production of an organic acid. (justia.com)

Additionally, ChIP-chip identified a number of novel direct FabR targets (the intergenic regions between hpaR / hpaG and ddg / ydfZ ) and yet putative direct targets ( i.a. genes involved in tRNA metabolism, ribosome synthesis and translation). (biomedcentral.com)

Since E1A and E1B jointly had a more pronounced effect, it was conceivable that genes in both regions acted together in the abrogation of the inhibitory effect of promoter methylations in the late E2A promoter. (uni-bielefeld.de)

In order to isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence of a few hundred or few thousand basepairs from the entire human genome. (addgene.org)

Subsequently, six of them were characterized by gene disruption. (biomedcentral.com)

Understand Nuclear Receptors through our introduction to this family of intracellular transcription factors that regulate gene expression. (indigobiosciences.com)

However, mitoribosome biogenesis does not depend on the methyltransferase activity of MRM2. (nature.com)

It is widely accepted that membrane voltage potentiates aminoglycoside activity, which is ascribed to voltage-dependent drug uptake. (elifesciences.org)
Throughout these early studies, uptake of the aminoglycosides was often treated as synonymous with bactericidal activity. (elifesciences.org)

MiRNAs appear to target about 60% of the genes of humans and other mammals. (microrna21.com)

Heterologous expression of an ATPase inhibitor completely eliminated bactericidal activity, while loss of the F-ATPase reduced the electrophysiological response to aminoglycosides. (elifesciences.org)

A derivative that lacks this domain, while it cannot repress the endogenous fushi tarazu gene, can still reduce the activity of the fushi tarazu autoregulatory enhancer, suggesting that it competes with endogenous Fushi Tarazu for binding sites in vivo. (biologists.com)

Only those cells that have successfully taken up the construct containing the CAT gene will survive and multiply under these conditions. (wikipedia.org)

We compared expression differences between pairs of the three vessels to determine whether MAPCAs display distinct expression patterns.Functional clustering analysis identified differences in gene expression, which were further analyzed by gene ontology classification. (stanford.edu)
These data indicate that GluK1 KARs are closely involved in functional regulation of the GABAergic network in the early postnatal hippocampus and suggest that its malfunction might influence adult oscillatory network activity and associated behaviors. (biomedcentral.com)

This is confirmed by showing that the in vivo activity of EFE depends on a domain that is required for active repression in culture. (biologists.com)
The in vivo produced transcripts are heterogeneous (due to any of promoter? (iresite.org)

In addition, using invitro methylation assay we identified 4 new substrates for PRMT6, extending the involvement of this enzyme to other cellular processes beyond its well-established role in gene expression regulation. (embl.de)

These results and further mapping with 5' deletion mutants from the -738/+95 region have demonstrated that an AP-1 site at -92/-86 is essential for basal expression of the gene. (uea.ac.uk)
Normal Fushi Tarazu target genes are affected as they are in fushi tarazu mutants. (biologists.com)

Such genes are called reporters because the characteristics they confer on organisms expressing them are easily identified and measured, or because they are selectable markers. (wikipedia.org)

The reactivation of a methylation-inactivated eukaryotic promoter by transactivating functions has general significance in that the value of a regulatory signal can be fully realized only by its controlled reversibility. (uni-bielefeld.de)

Arsenic exposure of human HaCaT keratinocytes induced nuclear accumulation of PRMT1 and PRMT4, histone H4R3 and H3R17 methylation proximal to the ARE, but not to the non-ARE regions of ferritin genes. (embl.de)

Both mechanisms result in increased activation of GABAergic neurons, suggesting that the physiological role of interneuronal GluK1 KARs is to act as a feedback mechanism to maintain the network excitability in a critical range during developmental and activity-dependent fluctuations in the levels of endogenous glutamate [ 11 ]. (biomedcentral.com)

The concept of genes as carriers of phenotypic information was introduced in the early 19th century by Gregor Mendel, who later demonstrated the properties of genetic inheritance in peas. (addgene.org)
In recent years, these powerful genetic tools have been used in higher vertebrates for transgenesis, insertional mutagenesis, and gene therapy applications. (biomedcentral.com)
With the use of this promoter, recombinants transformed to produce organic acids such as lactic acid in yeasts, including Saccharomyces , can be obtained by genetic engineering, and such recombinants are useful as those that have high productivity of organic acids. (justia.com)

For these reasons, it is thought that the variable N- terminal sequences may be responsible for the receptor-, species-, and cell type-specific effects as well as promoter context-dependent properties of NR transactivation. (indigobiosciences.com)

The N terminus of the NR, sometimes called the modulator, hypervariable or A/B domain, has transactivation activity, termed activation function 1 (AF-1). (indigobiosciences.com)
Conclusion】 The PERP 1 gene had the function of promoting granulosa cell apoptosis,and regulating its expression was of great significance for follicular development. (chvm.net)

The inactivating effect of sequence-specific promoter methylations was extensively studied by using the late E2A promoter of adenovirus type 2 (Ad2) DNA. (uni-bielefeld.de)

The thorough expression of every Inhibitors,Modulators,Libraries up regulated gene in pediatric AML was presented in Figure two along with the expression of down regulated genes was presented in Figure three. (microrna21.com)

The mechanisms that underlie and dictate the different biological outcomes of E2F-1 activity have yet to be elucidated. (embl.de)
Our results define an exquisite level of precision in the reader-writer interplay that governs the biological outcome of E2F-1 activity. (embl.de)
Moreover, antiradical activity, one of the typical biological activities of flavonoids, in propolis was also slightly higher than that in its botanical origin. (propoleo.cl)

Here, we show that GABAergic neurotransmission and spontaneous network activity is perturbed in the hippocampus of neonatal mice lacking GluK1 KARs selectively in GABAergic neurons. (biomedcentral.com)

In intensively reared food animals, antibiotics may be administered to whole flocks rather than individuals animals, and antimicrobial agents may be continually fed to food animals such as poultry, goats and cattle as growth promoters. (researchcub.info)

Next, we identified full-length nuclear HER3 association and regulation of a 122 bp region of the cyclin D1 promoter. (plos.org)

Next to UFA biosynthesis, a number of these direct targets and other indirect targets identified by transcriptomics (e.g. ribosomal genes, ompA , ompC , ompX , osmB , osmC, sseI ), could possibly contribute to the effect of FabR on biofilm formation. (biomedcentral.com)

One such target gene is repressed by EFE even where Fushi Tarazu is not expressed, suggesting that the repression is active. (biologists.com)
NRs bind to hormone response elements (HREs) in their target promoters through the DNA binding domain (DBD) or C domain. (indigobiosciences.com)

This method is an example of using cis-acting elements where the two genes are under the same promoter elements and are transcribed into a single messenger RNA molecule. (wikipedia.org)

Many genes that are regulated by one type of stress are also responsive to the other two. (allindianpatents.com)

HDACs one is vital in en hancing cytarabine induced apoptosis in pediatric AML, not less than partly mediated by Bim. (microrna21.com)

Anatomy14

Organisms12

Diseases3

Chemicals and Drugs111

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes88

Technology, Industry, Agriculture1

Information Science3

Proteins6

Transcriptional7

Recombinant9

Transfection2

Alcohol dehydrogenase1

Plasmids3

Inducible2

MRNA5

Protein19

Coli2

Bacteria8

Overexpression1

Reporter12

Locus3

Constructs1

Resistance1

Strain1

Genomic3

Preserved phenotypic1

Transient2

Exogenous1

Quantitative1

Induce1

Activates2

Putative1

Inhibitory1

Chromosomal1

Disruption1

Transcription factors1

Methyltransferase activity1

Uptake2

Humans1

Heterologous1

Enhancer1

Construct1

Functional2

Vivo2

Expression regulation1

Mutants2

Confer1

Regulatory1

Regions1

Developmental1

Genetic3

Receptor1

Function2

Sequence1

Libraries1

Biological3

Mice1

Growth1

Nuclear1

Targets1

Target2

Method1

Type1

Partly1