• SOX17
  • e and f) Sections through embryo in (d) indicate equal levels of SOX17 expression in all cells of the gut endoderm layer, regardless if emVE-derived (GFP-positive) or of the DE lineage (GFP-negative). (nih.gov)
  • By examining these effects in conjunction with those of Mixer- and Sox17β-activating and dominant inhibitory constructs, we further establish the likely relationships between GATA activity and these regulators in early development of the vertebrate endoderm. (pnas.org)
  • Inhibiting contractility resulted in an increase in the observed fibronectin FRET intensity ratio but also decreased the amount of phosphorylated nuclear SMAD2, leading to reduced expression of the definitive endoderm marker SOX17. (biologists.org)
  • parietal
  • This thesis looks at F9 derived parietal endoderm outgrowth as a model system to investigate the mechanisms that regulate migration of parietal endoderm in the establishment of extracellular embryonic tissues . (ccsu.edu)
  • The data presented here suggest that E-cadherin is expressed in parietal endoderm and that it localizes to the cell surface . (ccsu.edu)
  • In addition the data suggest that adherens junctions are forming between parietal endoderm cells , based on the localization of key adherens junction components . (ccsu.edu)
  • pancreatic
  • Pancreatic progenitor cells are multipotent stem cells originating from the developing fore-gut endoderm which have the ability to differentiate into the lineage specific progenitors responsible for the developing pancreas. (wikipedia.org)
  • vertebrate
  • Among vertebrate GATA proteins, the GATA-4/5/6 subfamily regulates aspects of late endoderm development, but a role for GATA factors in establishing the endoderm is unknown. (pnas.org)
  • We exploited this activity of END-1 to demonstrate that establishment of the vertebrate endoderm, like that of invertebrate species, also appears to involve GATA transcriptional activity. (pnas.org)
  • induction
  • Using genome editing technology, additional stem cell lines with mutations in both GATA6 alleles were generated and demonstrate a severe block in definitive endoderm induction. (upenn.edu)
  • The variable number of genes shows the multiple routes of induction of the developing endoderm, intrinsically within the endoderm (for example, notch signaling) or from the adjacent cardiac mesoderm (Sonic hedgehog protein inhibition by Fibroblast growth factor). (wikipedia.org)
  • epiblast
  • As is the case in several vertebrates, in the mouse there are two recognized sources of endoderm tissue, one arising from the PrE and the other from the pluripotent epiblast [ 2 , 3 ]. (royalsocietypublishing.org)
  • Transcription Factors
  • Evidence from the ecdysozoan protostomes ( 1 ) Drosophila and Caenorhabditis elegans indicates that activation of endoderm development is regulated by transcription factors of the GATA family in these species. (pnas.org)
  • gastrula
  • Cells migrating inward along the archenteron form the inner layer of the gastrula, which develops into the endoderm. (wikipedia.org)
  • endoderm (ĕn´dədûrm´) , in biology, inner layer of tissue formed in the gastrula stage of the developing embryo. (encyclopedia.com)
  • lineage
  • The C. elegans end-1 gene is expressed exclusively and early in the endoderm lineage ( 3 ), is able to program nonendodermal embryonic blastomeres to endodermal fate, and is the first zygotically transcribed gene known to specify endoderm in worms ( 4 ). (pnas.org)
  • cells
  • citation needed] The endoderm consists at first of flattened cells, which subsequently become columnar. (wikipedia.org)
  • ELT-2, a second gut-specific transcription factor expressed slightly later in the C. elegans endoderm, has two presumptive zinc fingers, one of which is similar to the GATA motif ( 5 ), and is required for maintenance of intestinal cells ( 6 ). (pnas.org)
  • Using the EP cell culture system to bypass the developmental block at the endoderm stage, cell lines with mutations in one or both GATA6 alleles were differentiated into β-like cells. (upenn.edu)
  • GATA
  • Similarly, mutations that eliminate the Drosophila GATA factor ABF/Serpent (dGATAb) result in the complete absence of the midgut, the only endoderm derivative in flies ( 7 , 8 ). (pnas.org)
  • cell
  • citation needed] In plant biology, endoderm corresponds to the innermost part of the cortex (bark) in young shoots and young roots often consisting of a single cell layer. (wikipedia.org)
  • From the inside out, the three cell layers are the endoderm, the mesoderm , and the ectoderm . (wisegeek.com)
  • The three cell layers are roughly equivalent to the position of structures in the fully formed human body, with the endoderm forming internal structures, the ectoderm forming external structures, and the mesoderm forming intermediary structures like the skeleton, skeletal muscle, and heart. (wisegeek.com)