CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (bio-techne.com)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
Our studies have demonstrated that several of the proteins encoded by cellular oncogenes function in fundamental aspects of gene regulation. (stanford.edu)
Alzheimer's possesses complex pathologies of heterogeneous nature counting proteins as one major factor along with enzymes and mutated genes. (benthamscience.com)
Based on previously established essentiality of genes in M. tb, four proteins implicated in acceleration of protein folding (PpiB), chaperone assisted protein folding (MoxR1), microbial replication (RipA) and host immune modulation (S-adenosyl dependent methyltransferase, sMTase) were selected. (bvsalud.org)
Most resulting proteins lead in techniques with IGFBPs, which plan methylated to modulate the CLASP of acts in the mode, react salt of IGFs to trim members for mice, promote cornea like supplements of IGFs, and be as encoding mitochondria here of IGFs. (evakoch.com)
The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). (antikoerper-online.de)
B29), and the -subunit of the E-mail: [email protected] interleukin (IL)-7 receptor [5, 6]. (lu.se)
The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). (creativebiomart.net)
It belongs to the type Icytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, areceptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. (creativebiomart.net)
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. (creativebiomart.net)
This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. (creativebiomart.net)
This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. (creativebiomart.net)
Human ciliary neurotrophic factor has been shown to interact with the Interleukin 6 receptor . (wikidoc.org)
By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. (meddic.jp)
STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. (thermofisher.com)
Protein12
Pyruvate kinase isozymes M1/M2 (PKM1/M2), also known as pyruvate kinase muscle isozyme (PKM), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an enzyme that in humans is encoded by the PKM2 gene. (wikipedia.org)
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. (antikoerper-online.de)
Recombinant Human IL-7 GMP Protein Bioactivity GMP-grade Recombinant Human IL-7 (Catalog # 207-GMP) stimulates proliferation of PHA-activated human peripheral blood lymphocytes. (bio-techne.com)
Recombinant Human IL-7 GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human IL-7 (Catalog # 207-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa. (bio-techne.com)
Recombinant Human IL-7 GMP Protein Mass Spectrometry MALDI-TOF analysis of GMP-grade Recombinant Human IL-7 (Catalog # 207-GMP). (bio-techne.com)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (bio-techne.com)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (bio-techne.com)
Mycobacterium tuberculosis (M. tb) gene Rv1515c encodes a conserved hypothetical protein exclusively present within organisms of MTB complex and absent in non-pathogenic mycobacteria. (bvsalud.org)
Recombinant human IL1R2 protein with His tag was expressed in HEK293. (creativebiomart.net)
Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene . (wikidoc.org)
The protein encoded by this gene is a polypeptide hormone and neurotrophic factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neural populations including astrocytes . (wikidoc.org)
Four transcript variants encoding three different isoforms (a proteoglycan, glycoprotein and cell surface protein) [2] have been found for this gene. (meddic.jp)
Antibody1
Cloud-CloneDescription: A polyclonal antibody rasied in rabbit against human Interleukin 7Polyclonal Antibody to Interleukin 7 (IL7) is. (proteomecommons.org)
Differentiation4
IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1-4). (bio-techne.com)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
VSV infection triggered rapid differentiation of blood monocytes into immature dendritic cells as well as their apoptosis, which depended on caspase 3/7 activation. (karger.com)
CDNA3
Human IL‑7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide (3). (bio-techne.com)
Interleukin-21 (IL-21) was first identified in 2000 through screening of a cDNA library from CD3+ human T cells [ 1 ]. (intechopen.com)
54 genes and eleven miRNAs have been concerned from the top 12 predicted functions, and a network produced by IPA showed the interactions amongst individuals miRNAs and mRNA. (trpvantagonists.com)
Hematopoietic3
Targeted gene deletion and radiation chimera analyses have provided insights into the molecular machinery underlying the development of secondary lymphoid organs, emphasizing the importance of crosstalk between hematopoietic and nonhematopoietic cells. (rupress.org)
We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
Tumor1
Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed. (at-the-casinos.com)
Coli3
Recombinant Human Interleukin-15（Recombinant Human IL-15細胞因子） is produced by our E.coli expression system and the target gene encodingbrAsn49-Ser162 is expressed. (at-the-casinos.com)
Recombinant Human Interleukin-7 is produced by our E.coli expression system and the target gene encodingbrAsp26-His177 is expressed. (at-the-casinos.com)
Recombinant Human Interleukin-18（Recombinant Human IL-18細胞因子） is produced by our E.coli expression system and the target gene encodingbrTyr37-Asp193 is expressed. (at-the-casinos.com)
Bone marrow1
IL-7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). (bio-techne.com)
Inhibits1
In the lactate production, the upregulated PKM2 is required and it leads to its contribution in inflammatory response, organ injury and septic death As a consequence, the removal of PKM2 in myeloid cells, administration of anti-PD-L1 or supplementation with recombinant interleukin -1 (IL-7) eases the microbial clearance, inhibits T cell apoptosis, reduce multiple organ dysfunction and reduce septic death in Bmal1-deficient mice. (wikipedia.org)
Variants2
Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described. (thermofisher.com)
Here, we report a systematic characterization of the interaction-modified vIL-6 variants and recombinant virus expressing the lytic phenotype selected variants. (ilgenetics.org)
Apoptosis1
STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. (thermofisher.com)
Expression10
The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. (antikoerper-online.de)
Recombinant Human IL-12 is produced by our mammalian expression system in human cells. (at-the-casinos.com)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
2) We have discovered a group of oncoproteins that are implicated in long-term maintenance of gene expression through their effects on the state of chromatin. (stanford.edu)
Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. (creativebiomart.net)
Gene expression and cytokine/chemokine secretion were assessed by real-time PCR and multiplex assay, respectively. (karger.com)
B) Luciferase reporter gene detection in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with expression in ZR75-1 and MDA-MB-231 cells, which were transfected with ZKSCAN5 shRNA, control shRNA, or ZKSCAN5 shRNA plus shRNA-resistant ZKSCAN5 (ZKSCAN5-R). The representative Western blot further showed the expression of ZKSCAN5. (bms-911543.com)
Interleukin-4 up-regulation of epidermal interleukin-19 expression in keratinocytes involves the binding of signal transducer and activator of transcription 6 (Stat6) to the imperfect Stat6 sites. (antibodies-online.com)
Deletion2
RQR8 is a 136-amino-acid epitope-based marker/suicide gene that enables clinical selection, cell tracking, and deletion in case of toxicity. (ashpublications.org)
Marker genes enable measurement of transduction and allow selection of transduced cells, whereas suicide genes allow selective deletion of administered T cells in the face of toxicity. (ashpublications.org)
Cells10
Reactivity has been confirmed by western blotting using cell lysates derived from human A431 cells (+/- EGF treatment), NIH 3T3 cells, MCF-7 cells, and HeLa cells. (thermofisher.com)
Interleukin-21 (IL-21) is produced by activated T cells and it plays many diverse roles by regulating the functions of normal and abnormal cells. (intechopen.com)
The gene that codes for this cytokine is switched on in an infected cell, and the interferon synthesized and secreted to surrounding cells. (wikidoc.org)
Notably, amid the dif ferentially expressed mRNAs predicted to become targeted through the differential miRNAs, had been some genes previously ex perimentally identified to get concerned in aneurysm forma tion or loss of vessel cells, this kind of as TGFBR1, MMPs and IL18 and so on. (trpvantagonists.com)
Marker genes enable detection and selection of T cells, whereas suicide genes enable selective destruction of T cells in case of toxicity. (ashpublications.org)
We have created a highly compact marker/suicide gene for T cells combining target epitopes from both CD34 and CD20 antigens (RQR8). (ashpublications.org)
Several studies have shown that adipocytes highly express CD1d, which presents lipid antigens to invariant natural killer T (iNKT) cells and stimulates the activation of iNKT cells ( 5 - 7 ). (frontiersin.org)
Specifically, the timely orchestration of pro- and anti-inflammatory responses, based on the activation and inhibition of various cell types, such as tissue-resident cells and different types of immune cells, can significantly impact wound repair [ 7 ]. (biomedcentral.com)
LTα knockout mice used here were designed to avoid the artifact involving TNF gene downregulation in myeloid cells, which occurred in the conventional LTα knockout mice. (rusimmun.ru)
A) Luciferase reporter genes were determined in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with Glutaminase-IN-1 different concentrations of reporter and myc-ZKSCAN5. (bms-911543.com)
Transcript1
In addition to the predominant monocistronic transcript originating from this locus, the gene is also cotranscribed with the upstream ZFP91 gene. (wikidoc.org)
Canine1
Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, and porcine IL-7. (bio-techne.com)
Infection1
M. s_Rv1515c was able to survive in the major organs of mice up to 7 weeks post-infection. (bvsalud.org)
Human5
1.0 x 10 8 units/mg, which is calibrated against the human IL-7 reference standard (NIBSC code: 90/530). (bio-techne.com)
Human and mouse IL-7 exhibit cross-species activity (2, 3). (bio-techne.com)
A recombinant version of human CNTF (rhCNTF), trade name Axokine, is a modified version with a 15 amino acid truncation of the C-terminus and two amino acid substitutions. (wikidoc.org)
Human herpesvirus 8 (HHV-8) virus interleukin-6 (vIL-6) is a cytokine that is secreted and most poorly translated into the endoplasmic reticulum (ER). (ilgenetics.org)
previous analysis of vIL-6 activity.IMPORTANCE Human herpesvirus 8 (HHV-8) -encoded viral interleukin-6 (vIL-6) was the first viral IL-6 homologues to be identified. (ilgenetics.org)
Vaccine1
Eight classical TB vaccine candidate antigens, 51 DosR regulon encoded antigens, 23 TB reactivation antigens, 5 TB resuscitation promoting factors (rpfs), 6 starvation and 24 other stress response-associated TB antigens were evaluated in the study. (biomedcentral.com)
Immunoglobulin1
Knockout studies of a similar gene in mouse suggesta role for this gene in regulating immunoglobulin production. (creativebiomart.net)
An abstract from the ASH annual meeting shows that during CAR-T cell manufacturing each stage has different functional and metabolic requirements and small changes can have large effects on the efficacy and side effects of CAR-T cell therapy [ 7 ] (Fig. 1 ). (biomedcentral.com)
Mutation1
A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease . (wikidoc.org)
Mice1
The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. (antikoerper-online.de)
Reagents1
A compact marker/suicide gene that utilizes established clinical-grade reagents and pharmaceuticals would be of considerable practical utility to T-cell cancer gene therapy. (ashpublications.org)
Antibodies1
[7] In the minority of subjects who did not develop the antibodies, weight loss averaged 12.5 pounds in one year, versus 4.5 pounds for placebo -treated subjects. (wikidoc.org)
Vector1
Preventing packaging of translatable P5-associated DNA contaminants in recombinant AAV vector preps. (harvard.edu)
Activity2
Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP IL-7. (bio-techne.com)
Caspase 3/7 activity was detected by luminescence assay. (karger.com)
STAT11
After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. (thermofisher.com)
Products1
PKM1 and PKM2 are different splicing products of the M-gene (exon 9 for PKM1 and exon 10 for PKM2) and solely differ in 23 amino acids within a 56-amino acid stretch (aa 378-434) at their carboxy terminus. (wikipedia.org)
Activation1
Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. (thermofisher.com)
Therapy1
We predict that RQR8 will make T-cell gene therapy both safer and cheaper. (ashpublications.org)
Mouse2
The OptEIA™ Set for mouse granulocyte macrophage colony stimulating factor (GM-CSF) contains the components necessary to develop enzyme-linked immunosorbent assays (ELISA) for natural or recombinant mouse GM-CSF in serum, plasma, and cell culture supernatants. (bdbiosciences.com)
Standardization: This immunoassay is calibrated against purified Baculovirus-expressed recombinant mouse GM-CSF. (bdbiosciences.com)
Days2
After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use. (creativebiomart.net)
Freshly prepare or use within 7 days of preparation, stored at 2-8°C. (bdbiosciences.com)