Encode repressor proteins. Medical search. Frequent questions

Other genes regulated by DtxR include those that encode proteins involved in siderophore-mediated iron uptake. (rcsb.org)
The mammalian Hes basic helix-loop-helix repressor genes play central roles in these processes by maintaining progenitor cells in an undifferentiated state and by regulating binary cell fate decisions. (biologists.com)
In many organs, cell proliferation and differentiation are antagonistically regulated by multiple basic helix-loop-helix (bHLH) activator and repressor genes. (biologists.com)
In particular,the Hes bHLH repressor genes play an essential role in the development of many organs by maintaining progenitor cells and by regulating binary cell fate decisions. (biologists.com)
In Rosetta(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene. (sigmaaldrich.com)
Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. (sigmaaldrich.com)
These interneurons expressed a unique combination of genes that encode transcription factors, receptors and neuropeptides and constituted around 30% of striatal interneurons in marmosets and humans. (nature.com)
Among CRE1 repressed transcripts, genes encoding unknown proteins and transport proteins were overrepresented. (biomedcentral.com)
In the following paragraphs, we will write genes in italics and their associated proteins in Roman. (wikidoc.org)
1) The alternation of protein levels of critical oncogenes or tumour suppressor genes, which causes by miRNAs deregulation may also lead to proliferative diseases such as cancer. (ukessays.com)
All genes in the ANTP-C and BX-C code for homeodomain proteins. (sdbonline.org)
In Drosophila, the majority of Notch target genes known so far is located in the Enhancer of split complex , encoding small basic helix-loop-helix (bHLH) proteins that presumably act as transcriptional repressors. (sdbonline.org)
The five genes described in this paper are arrayed between mbeta and m7 , both coding for bHLH proteins. (sdbonline.org)
Located on the X chromosome itself, Xist produces a protein that spreads up and down the chromosome during female embryonic development, turning off its genes. (sciencedaily.com)
and the University of California, Santa Cruz, Genome Browser, to look for genes or genomic regions of chromosomes 1 and 19 thought to produce proteins that interact with Xist. (sciencedaily.com)
RNA-seq identifies a novel set of TEAD4 target genes encoding muscle structural and regulatory proteins and those required for the unfolded protein response. (nih.gov)
Together these results show that TEAD factor activity is essential for normal C2C12 cell differentiation and define a novel and non-redundant role for TEAD4 in regulating expression of the unfolded protein response genes. (nih.gov)
In addition, viruses have been shown to regulate the metabolic processes of ecosystems by encoding auxiliary metabolic genes (AMGs) [ 11 ]. (biomedcentral.com)
Myb-Related Protein B (MYBL2), a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. (avivasysbio.com)
It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. (avivasysbio.com)
At the end of rapid growth, a co-ordinated transient up-regulation of about 100 genes, including many for ribosomal proteins, was seen in the parent strain but not the Δ bldA mutant. (biomedcentral.com)
Transcripts or proteins from a further 147 genes classified as bldA -influenced were mostly expressed late in culture in the wild-type, though others were significantly transcribed during exponential growth. (biomedcentral.com)
By interrogating microarray data from neuronal cells treated with inhibitors of different specificity, we selected two genes encoding histone macroH2A ( H2AFY2 ) and Polycomb group ring finger 2 ( PCGF2 ) that were specifically down-regulated by the inhibitors targeting HDACs1 and 3 versus the more selective inhibitors for further investigation. (frontiersin.org)
We characterize QmbHLH genes and identify the functions of QmbHLH proteins in Q. mongolica. (bvsalud.org)
Based on patterns of tissue-specific expression, protein interactions, and cis-element analysis, QmbHLH genes may be extensively involved in the growth and development of Q. mongolica. (bvsalud.org)
Of the 36,553 protein-coding genes predicted in the study, approximately 95% had functional annotations in public databases. (bvsalud.org)
Gene family evolution analysis of Q. mongolica revealed that the nucleotide-binding site (NBS)-encoding gene family related to disease resistance was significantly contracted, whereas the ECERIFERUM 1 (CER1) homologous genes related to cuticular wax biosynthesis was significantly expanded. (bvsalud.org)
Transcription factor Nrf2 is a key regulator of genes encoding phase II detoxification enzymes and antioxidant proteins in response to environmental chemical insults. (cdc.gov)
The SALL1 gene is part of a group of related genes that provide instructions for making proteins involved in the formation of tissues and organs before birth. (medlineplus.gov)
These proteins are transcription factors, which means they attach (bind) to specific regions of DNA and help control the activity of particular genes. (medlineplus.gov)
A shortage of functioning SALL1 protein, due to either type of mutation, likely impairs the regulation of genes that direct the development of many different organs and tissues before birth. (medlineplus.gov)
Las redes funcionan para en última instancia especificar la expresión de conjuntos concretos de GENES para estados, momentos o lugares específicos. (bvsalud.org)

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. (thermofisher.com)
The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. (genetex.com)
The Additional sex combs gene of Drosophila encodes a chromatin protein that binds to shared and unique Polycomb group sites on polytene chromosomes. (ox.ac.uk)

Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. (genetex.com)
The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. (avivasysbio.com)
Interacting DNA-encoded regulatory subsystems in the GENOME that coordinate input from activator and repressor TRANSCRIPTION FACTORS during development, cell differentiation, or in response to environmental cues. (bvsalud.org)

The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7 lac promoter in the absence of inducer. (sigmaaldrich.com)
It regulates the transcription of the cI protein and the Cro protein. (wikidoc.org)
The Dl and Twist proteins appear to make synergistic contact with the basal transcription complex, so that snail is activated throughout the presumptive mesoderm in response to both peak and high levels of the Dl gradient. (sdbonline.org)
MLX may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4.The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. (prosci-inc.com)
To reduce the effects of protein toxicity on bacterial cell growth prior to induction, the level of basal transcription that occurs in the absence of induction ("leakiness") should be repressed as much as possible, and the number of generations before induction should be kept to a minimum. (qiagen.com)

The GETI has a novel scaffold based on bacterial repressors, a class of transcriptional regulators that are critical for bacteria to respond to environmental chemicals. (caltech.edu)
The assessment of potential transcriptional regulators in ENCODE ChIP-seq database identified transcriptional repressor E2F6 as a possible negative regulator of MDM2 expression. (tmc.edu)

Both Int and IHF bind to attP and form an intasome , a DNA-protein-complex designed for site-specific recombination of the phage and host DNA. (wikidoc.org)
Members of this family have been shown to bind to KNOX and BELL- like TALE class homeodomain proteins. (utoronto.ca)
I repurposed an antibiotic-sensing repressor protein to bind a neurotransmitter, melatonin, using machine-learning-guided directed evolution. (caltech.edu)
protein_bind 1657. (gentaur-italy.com)
protein_bind 1731. (gentaur-italy.com)
protein_bind 109. (gentaur.shop)
protein_bind 183. (gentaur.shop)

Alternatively spliced transcript variants encoding different isoforms exist. (wikipedia.org)
Three transcript variants encoding different isoforms have been found for this gene. (genetex.com)

The pQE-80L vectors have a cis- lacIq gene that overexpresses the lac repressor, in addition to a lacI repressor gene present in trans on a separate pREP4 plasmid. (qiagen.com)

Rare, protein-truncating variants substantially increase the risk for mental illnesses like schizophrenia. (sciety.org)

A filamentous bacteriophage (CTXf) integrated in the Vibrio cholerae chromosome encodes the cholera toxin, and Matthew Waldor (New England Medical Center, Boston, MA) described another phage (RS1) that flanks the CTXf prophage in the bacterial chromosome and is important for the CTXf prophage propagation. (cdc.gov)
The repressor found in the phage lambda is a notable example of the level of control possible over gene expression by a very simple system. (wikidoc.org)
The lambda phage will remain in the lysogenic state if cI proteins predominate, but will be transformed into the lytic cycle if cro proteins predominate. (wikidoc.org)

However, she says, the gene responsible for this repressor, or even its approximate location in the human genome, has been unclear. (sciencedaily.com)

AN is expressed at low levels in all organs and the AN protein is localized in the cytoplasm. (nih.gov)

Contains the pLacI plasmid producing extra Lac repressor. (sigmaaldrich.com)
Expression host strains for pETBlue and pTriEx vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. (sigmaaldrich.com)

This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. (wikipedia.org)

Both proteins were ubiquitinated in the cytoplasm. (cdc.gov)

This interference prevents the normal proteins from entering the nucleus to regulate gene activity. (medlineplus.gov)

The advantages of cell-free expression systems include time savings, the possibility to produce proteins that are toxic and the ability to adapt reaction conditions to the requirements of the synthesized protein. (qiagen.com)

The binding of miRNA- protein complexes to mRNA causes translation inhibition or destabilisation of target transcripts. (ukessays.com)
1) This is resulting in the downregulation of the protein encoded by mRNA. (ukessays.com)
Using a newly developed human neuronal cell model, derived from patient-induced pluripotent stem cells, we find that 2-aminobenzamide histone deacetylase (HDAC) inhibitors increase FXN mRNA levels and frataxin protein in FRDA neuronal cells. (frontiersin.org)
In MEF cells with wild type Nrf2 and mouse hepa 1c1c7 cells, Cd increased the Nrf2 protein level but not the Nrf2 mRNA level dose-dependently. (cdc.gov)

Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. (thermofisher.com)
Activation domains influence the way proteins interact with the trancription apparatus of the cell (Dawson, 1995). (sdbonline.org)
The malfunctioning protein may also interact with other proteins, disrupting their function. (medlineplus.gov)

Proteins were shown to be linear polymers of amino acids and the first three-dimensional structures of proteins solved. (taylorfrancis.com)
The order of nucleotides in the DNA was hypothesized and confirmed to instruct the sequence of amino acids in proteins via a temporary ('messenger') RNA copy of the gene and adaptor 'transfer RNAs' that connected the two. (taylorfrancis.com)
Asx was cloned by transposon tagging and encodes a protein of 1668 amino acids containing an unusual cysteine cluster at the carboxy terminus. (ox.ac.uk)

This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. (thermofisher.com)
The retroviral oncogene v-myb, and its cellular counterpart c-myb, encode nuclear DNA-binding proteins. (embl.de)
but this includes many previously unknown effects at a variety of different levels, including post-translational changes in proteins and global cellular physiology. (biomedcentral.com)
For example, some research indicates that the abnormally short SALL1 protein interferes with proteins that control the formation of cellular structures called cilia. (medlineplus.gov)

The lactose utilization (lac) operon of Escherichia coli was shown to specify three enzymes and a regulatory locus, initially posited to express an RNA, but subsequently found to encode a DNA-binding repressor protein that is displaced upon lactose binding. (taylorfrancis.com)

Here we show that the AN gene encodes a C-terminal binding proteins/brefeldin A ADP-ribosylated substrates (CtBP/BARS) related protein. (nih.gov)

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
The newly minted 'Central Dogma' of molecular biology asserted that 'DNA makes RNA makes proteins', with RNA tacitly but firmly relegated to an ephemeral intermediate. (taylorfrancis.com)

Functions as a transcriptional repressor that suppresses cell elongation. (utoronto.ca)
Further characterization showed that susa2-2 only suppresses the autoimmunity mediated by either CHS1-SOC3 or TN2-SOC3 paired NLR proteins, indicating that SUSA2 is specifically involved in NLR protein SOC3-mediated immunity. (ubc.ca)

Results Protein-altering de novo mutations in PURA were identified in four subjects. (bmj.com)
Most SALL1 gene mutations involved in this disorder lead to the production of an abnormally short version of the SALL1 protein that malfunctions within cells. (medlineplus.gov)
Some studies suggest that mutations that reduce the amount of SALL1 protein are responsible for milder cases of Townes-Brocks syndrome, and mutations that result in an abnormally short, malfunctioning SALL1 protein underlie the more severe cases of the condition. (medlineplus.gov)

The most likely explanation for this phenomenon, Migeon reasons, was that a protein that represses the X chromosome silencing activity of Xist was working overtime, allowing more than one X chromosome to remain activated. (sciencedaily.com)
To identify the likely location of the repressor protein and the gene that codes for it, the researchers started by looking at cells from human embryos with different forms of chromosomal trisomy, a condition in which cells carry three copies of a particular chromosome instead of two. (sciencedaily.com)

There was also an upregulation in expression of positive regulators and key components of the AMPK pathway, autophagy, proteasome function, and the unfolded protein response. (nature.com)
In agreement with its activity as an RNA-binding protein, loss of RBM12 leads to altered gene expression, including that of multiple effectors of established significance within the receptor pathway. (sciety.org)

Large ribonucleoprotein complexes, termed 'ribosomes', were identified as the sites of protein synthesis. (taylorfrancis.com)

Trisomies of these chromosomes were missing, suggesting that the repressor might be located on one of them," says Migeon. (sciencedaily.com)
The team reasoned that if the repressor was in a region that was duplicated, it would work overtime and turn off Xist on both X chromosomes, leaving both the X chromosomes active and selectively changing the survival of male vs. female embryos. (sciencedaily.com)

The diphtheria toxin repressor (DtxR) from Corynebacterium diphtheriae regulates the expression of the gene on corynebacteriophages that encodes diphtheria toxin (DT). (rcsb.org)
Nevertheless Hair mostly regulates the appearance of a big group course Ib or NrdR comprises an N-terminal nonclassical zinc-binding area and a distinctive C-terminal ATP-cone domains [1] which classifies NrdR inside the ATP-cone category of proteins. (immune-source.com)

Interestingly, functionally redundant SNIPER1 and SNIPER2 can control the protein levels of diverse sNLRs and the interactions between SNIPER1 and sNLRs appear to be through the common nucleotide-binding (NB) domains of sNLRs. (ubc.ca)
Plant intracellular nucleotide binding and leucine-rich repeat proteins (NB-LRR, NLRs) function as immune receptors to detect microbial pathogens directly or indirectly. (ubc.ca)

Tac strong promoter activated the expression of mCherry red fluorescent protein. (gentaur-italy.com)

RstC and RstR appear to form intracellular aggregates that prevent the repressor from binding to its operators. (cdc.gov)

Novagen Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. (sigmaaldrich.com)

These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. (prosci-inc.com)
Expression of the TEA/ATTS DNA binding domain that acts a dominant negative repressor of TEAD factors in C2C12 myoblasts inhibits their differentiation, while selective shRNA knockdown of TEAD4 results in abnormal differentiation characterised by the formation of shortened myotubes. (nih.gov)

Second, I describe the development of Automated Pairwise Peptide-Receptor AnalysIs for Screening Engineered proteins (APPRAISE). (caltech.edu)
This computational pipeline predicts the receptor binding propensity of engineered proteins based on competitive modeling and physics-grounded analysis. (caltech.edu)
I show that APPRAISE is capable of distinguishing between receptor-dependent and receptor-independent adeno-associated viral vectors and ranking various engineered proteins, such as miniproteins binding to the SARS-CoV-2 spike and nanobodies binding to a G-protein-coupled receptor. (caltech.edu)
A melatonin indicator was then created by integrating the repurposed receptor with a fluorescent protein. (caltech.edu)
In chapter 2, by using CRISPR/Cas9 gene editing, genetic analysis and biochemical assays, I identified the differential pairings of typical NLR receptor SOC3 with atypical NLR proteins CHS1 or TN2 to guard the homeostasis of the E3 ligase SAUL1. (ubc.ca)

The vectors in the cis-Repressed pQE Vector Set are similar to those in the N-Terminus pQE Vector Set, but additionally express the laclq gene product that represses protein expression prior to IPTG induction. (qiagen.com)
pQE vectors provide high-level expression of protein in E. coli and add the small 6xHis affinity tag to protein expression constructs. (qiagen.com)
For very toxic proteins, we recommend using the pQE-80L series of expression vectors in the M15[pREP4] E. coli host strain . (qiagen.com)

Once a protein-encoding DNA insert has been incorporated into the vector, it is transformed into E. coli cells and protein expression is induced by the addition of IPTG to the growth medium. (qiagen.com)
How can I express toxic protein in E. coli? (qiagen.com)

Replacing either of the salt-bridge partners with an alanine reduces repressor activity substantially and it has been inferred that the salt bridge could possibly control the wedge angle between the DNA-binding domain and the dimerization domain, thereby modulating repressor activity. (rcsb.org)

SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)

The DNA of the prophage that is expressed in that state codes for proteins that look out for signs of stress in the host cell. (wikidoc.org)
T he presence and activity of various proteins are required in the cell cycle progression. (ukessays.com)
1) The regulation of these protein levels is vital for the understanding of the cell cycle control and its dysregulation. (ukessays.com)
2) Therefore, the proper control of protein levels is crucial for the cell cycle. (ukessays.com)
After cell lysis, 6xHis-tagged proteins are purified by Ni-NTA affinity chromatography under native or denaturing conditions. (qiagen.com)
Another alternative is to use cell-free protein biosynthesis systems , like the EasyXpress Protein Synthesis Kit . (qiagen.com)
Launch For all microorganisms well-timed and temporal legislation of gene appearance and its own translation to proteins level is essential for cell proliferation. (immune-source.com)

This combination of two repressor modules results in highly efficient suppression of recombinant protein expression prior to induction and gives the best chance of successful expression of toxic proteins. (qiagen.com)

Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)
This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. (embl.de)

ZNF274+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH) FactorBook ZNF274 This article incorporates text from the United States National Library of Medicine, which is in the public domain. (wikipedia.org)

Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)
This difference is reflected in the activation domains of the respective proteins. (sdbonline.org)
There are 139666 SANT domains in 88628 proteins in SMART's nrdb database. (embl.de)

The encoded protein is involved in regulation of vascular smooth muscle cells (VSMC) contractile proteins. (nih.gov)

Anatomy7

Organisms8

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes61

Information Science3

Genes32

Binds3

Activator3

Transcription5

Transcriptional regulators2

Bind7

Transcript variants2

LacI1

Variants1

Phage3

Genome1

Localized in the cytoplasm1

Plasmid2

Zinc finger domains1

Cytoplasm1

Nucleus1

Produce proteins1

MRNA4

Interact3

Amino acids3

Cellular4

Regulatory1

Brefeldin1

Molecular2

Suppresses2

Mutations3

Chromosome2

Pathway2

Complexes1

Chromosomes2

Regulates2

Nucleotide2

Promoter1

Intracellular1

Codons1

Differentiation2

Receptor5

Vectors3

Coli2

Substantially1

Classification1

Cell7

Expression1

Genomic2

MeSH1

Domains3

Regulation1