Encode membrane proteins genes bacterial. Medical search. Frequent questions

In Staphylococcus aureus, the phage-mediated acquisition of mobile genetic elements (MGEs) that encode virulence and antibiotic resistance genes largely contribute to its evolutionary adaptation and genetic plasticity. (bvsalud.org)
The bacterial pathogens within the RSSC produce exopolysaccharide (EPS), a highly complicated nitrogen-containing heteropolymeric polysaccharide, as a major virulence factor. (ppjonline.org)
These results indicate that the NDP-sugar epimerase gene is required for EPS production and bacterial virulence in R. pseudosolanacearum . (ppjonline.org)
The target of the nanobodies was identified as the major outer membrane protein, a porin that contributes to bacterial virulence and viability. (biomedcentral.com)
This diversification was associated with the acquisition of genes which increased the virulence of Salmonella and enabled it to interact with its hosts and colonise the intestinal tract of animals in a different way than E. coli did. (biomedcentral.com)
The roles of different SPI genes in the virulence S. enterica for chickens are less well understood. (biomedcentral.com)

This is the first report of any bacterial species possessing two genes for homologs of this major outer membrane protein. (shengsci.com)
The other, ofr, is within the ompA gene which encodes the major outer membrane protein. (edu.au)

To determine whether actA itself, plcB or other co-transcribed downstream regions are involved in actin assembly, mutations in the appropriate genes were generated. (wikipedia.org)
Resistance to quinolones occurs through chromosomal mutations in the genes encoding these enzymes and by porin and efflux mutations. (cdc.gov)
Mutations that result in alterations of the outer membrane porin proteins of Gram-negative organisms lead to decreased permeability of the drug through the outer membrane so less drug reaches the target enzyme. (cdc.gov)
Resistance can develop due to mutations, or by bacteria acquiring new genetic material encoding resistance. (futurelearn.com)
In addition, diminished expression of outer membrane proteins, mutations in topoisomerases, and up-regulation of efflux pumps also play an important part in antibiotic resistance. (microbiologyjournal.org)
Mutations in the Caenorhabditis elegans dpy-13 (dumpy) gene result in a short, chunky body shape. (ncsu.edu)
Besides the ability of bacteria to acquire antimicrobial resistance via horizontal gene transfer or spontaneous mutations, they can also be intrinsically resistant to antimicrobials ( Cox and Wright, 2013 ). (frontiersin.org)
For each of these factors we will construct isogenic non-polar mutations in the structural gene and test the mutants for decreased adherence in differential intestinal epithelial cell lines, freshly harvested human intestinal epithelial cells, and in a gnotobiotic piglet model of disease. (usda.gov)
[ 10 ] Other genetic causes involve mutations in genes regulating the packaging, transport, or release of cytotoxic granules. (medscape.com)

The glycerol facilitator is one of the few known examples of bacterial solute transport proteins that catalyse facilitated diffusion across the cytoplasmic membrane. (nau.edu)
1998) Patch clamp studies on ion pumps of the cytoplasmic membrane of Escherichia coli . (aimspress.com)
The SPI-1 encoded T3SS is required for the transport of S. enterica proteins across the cytoplasmic membrane of a host cell into its cytosol where they induce cytoskeletal rearrangements resulting in the uptake of S. enterica even by non-phagocytic cells [ 6 ]. (biomedcentral.com)

Quinolones inhibit two enzymes that are required for bacterial DNA synthesis, i.e. (cdc.gov)
Originally discovered from members of the nematode symbiotic genus Photorhabdus, the biosynthetic gene cluster (BGC) encoding for the synthesis of darobactin A can also be found in other γ-proteobacterial families. (sdsc.edu)
The susceptibility of the spores of sfr and php2 mutants to such a physical and chemical stress implies an important role of these genes in spore wall synthesis. (swan.ac.uk)
Here we show that staphylococcal phages produce a structured RNA transcribed from the terminase subunit genes, termed CBASS-activating bacteriophage RNA (cabRNA), which binds to a positively charged surface of the CdnE03 cyclase and promotes the synthesis of the cyclic dinucleotide cGAMP to activate the CBASS immune response. (bvsalud.org)
Ribosomes, the structures where protein synthesis is catalyzed, are the targets of many other Streptomyces antibiotics such as spectinomycin, tetracycline, and streptomycin. (icr.org)
Following me will be Corinna Tuckey, who will describe the utility and advantages of the PURExpress in vitro protein synthesis kit. (neb.com)
Bacterial spheroplasts do not divide but they grow and enlarge with DNA replication in a broth containing an inhibitor of peptidoglycan synthesis and high salt concentration. (aimspress.com)

Based on the Escherichia coli OmpA model, an eight-stranded amphipathic beta-barrel model for the membrane assembly of the N-terminal half of OmpAI and OmpAII was predicted. (shengsci.com)
An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. (shengsci.com)
Expression of a Saccharomyces cerevisiae photolyase gene in Escherichia coli. (shengsci.com)
A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. (shengsci.com)
The current study elucidates and compares the changes in the membrane proteome of Escherichia coli in response to a range of alcohols. (biomedcentral.com)
In this regard, Escherichia coli is of special interest, since it is genetically tractable and the most well-understood bacterial species that is widely considered for commercial biofuel production [ 6 , 7 ]. (biomedcentral.com)
The genes encoding the glycerol facilitator, glpF, and glycerol kinase, glpK, were isolated on a 4.5 kb EcoRl fragment from a chromosomal mini-library by functional complementation of an Escherichia coli glpK mutant after establishing a map of the chromosomal glpFK region with the help of a PCR-amplified glpK segment. (nau.edu)
The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli . (bionity.com)
In this study, choline dehydrogenase ( betA ) gene encoding for glycine betaine biosynthesis in Escherichia coli isolated from salted shark ( Scoliodon sp. (scialert.net)

In molecular biology, generalized transduction is routinely used as a technique to manipulate and construct bacterial strains. (bvsalud.org)
And then Mehmet Berkman will highlight the SHuffle line of E. coli strains for producing proteins with complex disulphide bonds. (neb.com)
Common to all strains of A. baumannii is chromosomally encoded cephalosporinase (AmpC type). (microbiologyjournal.org)
The proteins belong to more than 4,000 new biological families predicted to be involved in, among other processes, the warfare waged among different bacterial strains as they vie for primacy in coveted biological niches, the cell-to-cell communication between microbes and their unwitting hosts, and the critical day-to-day housekeeping duties that keep the bacteria happy and healthy. (technologynetworks.com)
2000), so that as the different parts of penicillin biosynthesis operons in and various other antibiotic-producing strains of includes three open up reading structures encoding SulP polypeptides. (exposed-skin-care.net)
We recently showed that in vitro expression of the intimin adhesin and the type III secretion system encoded on the LEE pathogenicity island is positively regulated by autoinducer molecules expressed by normal flora E. coli strains, i.e., quorum sensing. (usda.gov)

The fluoroquinolone susceptibility profile for each clinical isolate is determined by the number and location of mutational changes in specific enzyme target sites, porin proteins, and efflux mechanisms. (cdc.gov)
The gene tcp orf3*, encoding for the teicoplanin mannosyltransferase, was cloned from the deoxyribonucleic acid (DNA) of a.teichomycetus and, based on this, we were able to express the enzyme in e. coli as host. (europa.eu)
From its sequence we foresaw that the enzyme could be an integral membrane protein, therefore difficult to isolate and handle. (europa.eu)
The enzyme contained 2 g-atoms of iron per mol of protein. (shengsci.com)
Histone deacetylase 7 is an enzyme that in humans is encoded by the HDAC7 gene. (hdacassay.com)
Duck acyl-[acyl-carrier protein] hydrolase, medium chain (EC 3.1.2.14 ), an enzyme that may be associated with peroxisome proliferation and may play a role in the production of 3-hydroxy fatty acid diester pheromones. (expasy.org)
Phenmedipham hydrolase (phenylcarbamate hydrolase), an Arthrobacter oxidans plasmid-encoded enzyme (gene pcd) that degrades the phenylcarbamate herbicides phenmedipham and desmedipham by hydrolyzing their central carbamate linkages. (expasy.org)
The eukaryotic proteins are more complicated than the bacterial enzyme complexes. (tcdb.org)
Electron cryoEM structures of mitochondrial, chloroplast, and bacterial ATP synthases have revealed the architecture of the FO region, helping to explain the mechanisms of proton translocation, dimerization of the enzyme in mitochondria, and cristae formation. (tcdb.org)

Transposons, also known as jumping genes, are short sequences of DNA that physically move within a genome, mutating genes by jumping into them. (scienceblog.com)
If the mutation causes an interesting effect, scientists can identify the mutated gene by locating the transposon, sequencing the DNA surrounding the transposon and mapping its location in the genome. (scienceblog.com)
The genome of Caulobacter crescentus is structured in a circular chromosome and is comprised of 4,016,942 bp encoding for 3,767 genes. (kenyon.edu)
It was found that the C . crescentus genome encodes for more two-component signal transduction proteins that assist in cell cycle progression than any other bacterial genome sequenced so far. (kenyon.edu)
Four such pairs of genes are found in the genome of filamentous differentiating Streptomyces coelicolor. (swan.ac.uk)
A 146 base pair sequence, encoding amino acids 56-103, is unique in the C. elegans genome, and it hybridizes to a 1 kb mRNA of moderate abundance. (ncsu.edu)
The genome of the geminivirus tomato golden mosaic virus (TGMV) consists of two circular DNA molecules designated as components A and B. The A component contains the only virally-encoded function required for autonomous replication in infected plant cells. (ncsu.edu)
The bacterial genome is like a book with long strings of letters, only some of which encode the information necessary to make proteins," Bhatt said. (technologynetworks.com)
The phage coat is primarily assembled from a 50 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome . (bionity.com)
However, deletion of a phage protein (p3) prevents full escape from the host E. coli , and phage that are 10-20X the normal length with several copies of the phage genome can be seen shedding from the E. coli host. (bionity.com)
Two phage gene products play critical roles in the next stage of the phage life cycle, namely amplification of the genome. (bionity.com)
CtGEM typing was developed to subdivide the bacterial species Chlamydia trachomatis on the basis of genome phylogeny and anatomical tropism. (edu.au)
One, rg1, is within a hypothetical gene annotated as Jali-1891 within the C. trachomatis B_Jali20 genome. (edu.au)

Based on patterns in their DNA sequences, these genes likely encode proteins in the bacterial membrane. (scienceblog.com)
Since many of today's expression vectors encode Shine-Dalgarno sequences it is easy to overlook problems with translation initiation. (neb.com)
We've been more likely to make an error than to guess correctly when trying to predict which bacterial DNA sequences contain these very small genes," Bhatt said. (technologynetworks.com)
Open up in another window Physique 1 Phylogenetic romantic relationship of BIIE 0246 supplier human being SLC26 polypeptides generated with Jalview (http://www.jalview.org) using NCBI proteins sequences listed in Desk 1. (exposed-skin-care.net)
The betA gene sequence reported in this study contains several base substitutions with that of reported sequences in GenBank, resulting in the altered amino acid sequences of the translated proteins. (scialert.net)
Moreover, the sequence analysis of choline dehydrogenase from our isolate shows several base substitutions with that of reported sequences in GenBank, resulting in the altered amino acid sequences of the translated proteins. (scialert.net)

Identification of emerging bacterial pathogens generally results from a chain of events involving micros- copy, serology, molecular tools, and culture. (cdc.gov)
They correspond to functions allowing opportunistic pathogens to colonise the mucus layers, breach the host barriers and invade the mucosae, which could still be aggravated by decreased host-derived pancreatic zymogen granule membrane protein GP2 in CD patients. (bmj.com)
There is great need for therapeutics against multi-drug resistant, Gram-negative bacterial pathogens. (sdsc.edu)
Staphylococcus aureus is one of the most relevant mastitis pathogens in dairy cattle, and the acquisition of antimicrobial resistance genes presents a significant health issue in both veterinary and human fields. (bvsalud.org)
The various zoological phyla have played with a toolbox of genes encoding receptors, adaptors, kinases, transactivators, and antimicrobial peptides according to their own agendas, essentially their own environments and pathogens," he said. (newswise.com)
At the crux of the Hoffmann lab body of work is the Toll receptor, a protein that straddles the cell membranes of immune system "sentinel" cells, detecting molecular nametags of pathogens from the outside and transducing messages to the inside, activating and orchestrating defense against infection. (newswise.com)

As such it was found to be located in soluble e. coli membranes that we were able to isolate by ultracentrifugation for in vitro testing. (europa.eu)
Expression of ompAI and ompAII separately in E. coli indicated that both genes could be independently transcribed from their own promoters and that both gene products were assembled into the E. coli outer membrane. (shengsci.com)
Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a. (shengsci.com)
Although alcohol toxicity increased exponentially with alcohol chain length (2-6 carbon), similar stress responses were observed in the inner and outer membrane proteome of E. coli in the presence of 2-, 4- and 6-carbon alcohols at the MIC 50 . (biomedcentral.com)
Since alcohol primarily targets the integrity of the membrane, maintenance of outer membrane OmpC levels in the presence of alcohol might help in the survival of E. coli to higher alcohol concentrations. (biomedcentral.com)
The study provides important information about the membrane protein responses of E. coli to a range of alcohols, which can be used to develop targeted strategies for increased microbial alcohol tolerance and hence bioalcohol production. (biomedcentral.com)
I will start by outlining the most common problems encountered during the expression of recombinant proteins in E. coli and specific solutions will be given for each problem. (neb.com)
A gene can be ordered, it is optimized for E. coli expression and it can be at your lab within three weeks. (neb.com)
These form the rounded tip of the phage and are the first proteins to interact with the E. coli host during infection. (bionity.com)
Filamentous phage use a bacterial structure known as the F pilus to infect E. coli , with the M13 p3 tip contacting the TolA protein on the bacterial pilus. (bionity.com)

Several two-component signal transduction proteins are involved in the cell cycle progression by accumulating at one or both poles "in a spatial and temporal pattern that is reproduced during each cycle" (Jacobs-Wagner 2003). (kenyon.edu)

Depending on the degree of dehydration, dry mucous membranes, hypotension, prolonged capillary refill time, and poor skin turgor may be present. (medscape.com)
It can be part of the bacterial flora of the skin, particularly in moist regions such as the axillae, groin and toe webs, and up to 43% of healthy adults can have colonization of skin and mucous membranes with higher rates among hospital personnel and patients. (microbiologyjournal.org)

Cyclic oligonucleotide-based antiphage signalling systems (CBASS) protect prokaryotes from viral (phage) attack through the production of cyclic oligonucleotides, which activate effector proteins that trigger the death of the infected host1,2. (bvsalud.org)

These range from receptors for microbial aggressors, to intracellular signaling cascades, which control the expression of immune response genes, and the genes encoding potent antimicrobial peptides," Dr. Hoffmann explained. (newswise.com)

Many proteins, largely derived from Bacteroides species, were over-represented, while under-represented proteins were mostly from Firmicutes and some Prevotella members. (bmj.com)
The β-lactam family of drugs target the bacterial cell wall and therefore, would not have any effect on Mycoplasma species. (futurelearn.com)
Matthew Barber and Nels Elde from the University of Utah School of Medicine compared transferrin-encoding genes from 21 primate species, and found remarkable variation at sites known to interact with a bacterial transferrin binding protein, TbpA. (the-scientist.com)
Two bacterial species, Haemophilus influenzae and Neisseria meningitidis , are among the main causes of meningitis, a life-threatening inflammation of the membranes surround the brain and spinal cord. (the-scientist.com)
Acinetobacter species cause infections that are difficult to control due to multi-drug resistance and are noted for their intrinsic resistance to antibiotics and for their ability to acquire genes encoding resistance for the production of beta-lactamases and Aminoglycoside-modifying enzymes. (microbiologyjournal.org)
Ralstonia solanacearum species complex (RSSC) is a soil borne plant pathogen causing bacterial wilt on various important crops, including Solanaceae plants. (ppjonline.org)

ActA has a molecular weight of 70,349 Da and is a surface protein. (wikipedia.org)
Conclusions This study provides the first evidence that quantifiable bacterial protein signals are associated with CD, which can have a profound impact on future molecular diagnosis. (bmj.com)
Each SEDS gene is invariably linked with a cognate class B high-molecular weight penicillin-binding protein (HMW PBP) gene. (swan.ac.uk)
Molecular chaperones in the matrix exert multiple functions in translocation, sorting, folding, and assembly of newly imported proteins. (nih.gov)
Molecular structure and transformation of the glucose dehydrogenase gene in Drosophila melanogaster. (ncsu.edu)
The glycerol facilitator and glycerol kinase were identified in a T7 expression system as proteins with apparent molecular masses of 25 and 56 kDa, respectively. (nau.edu)
A research fellowship is available at the Molecular Ecology Group of the CNR-IRSA, Verbania, in the frame of the PRIN2020 project "IdeARG" - Identification and isolation of antibiotic resistance gene containing freshwater bacteria in an eco-evolutionary frame - funded by MIUR. (simgbm.it)
The main aim of our research is to understand the molecular details and physiological roles of the multitude of water channel-like proteins present in plants and animals. (lu.se)
Molecular Membrane Biology 2013, 30 (3):246-260. (lu.se)
Mark L., Lee W. H., Villoutreix B. O., Proctor D., Blackbourn, D., Spiller B. O. and Blom A. M. (2004) KSHV complement control protein mimics human molecular mechanisms for inhibition of the complement system. (lu.se)

The DNA sequence reveals that dpy-13 is a member of the collagen multi-gene family, and it could encode a polypeptide of 302 amino acids. (ncsu.edu)
Transcription of the wheat chloroplast gene that encodes the 32 kd polypeptide. (ncsu.edu)
Schweizer, HP , Jump, R & Po, C 1997, ' Structure and gene-polypeptide relationships of the region encoding glycerol diffusion facilitator (glpF) and glycerol kinase (glpK) of Pseudomonas aeruginosa ', Microbiology , vol. 143, no. 4, pp. 1287-1297. (nau.edu)
Desk 1 The SLC26 Multifunctional Anion Exchanger /Anion Route gene Family includes a one SulP polypeptide, YchM. (exposed-skin-care.net)
Built YchM deletion exhibited artificial lethality with multiple fatty acidity biosynthesis genes, as well as the YchM polypeptide bodily interacted numerous proteins from the fatty acidity biosynthetic pathway. (exposed-skin-care.net)

Maintaining dosing is important for some antibiotics in minimising the selection of antibiotic resistant mutants in high density bacterial populations. (futurelearn.com)
These fascinating mutants are a hands-on way to analyze genes. (kenyon.edu)
Construction of disruption mutants of each SEDS gene revealed ftsW, sfr and rodA2 are dispensable for growth and survival of S. coelioclor, whereas rodA is essential. (swan.ac.uk)
Complete cross-wall formation in the vegetative hyphae of all three fts mutants imply that the typical bacterial divisome functions specifically during non-essential sporulation septation. (swan.ac.uk)
To identify genes in EPS production beyond the EPS biosynthetic gene operon, we selected the EPS-defective mutants of R. pseudosolanacearum strain SL341 from Tn5-inserted mutant pool. (ppjonline.org)
Among several EPS-defective mutants, we identified a mutant, SL341P4, with a Tn5-insertion in a gene encoding a putative NDP-sugar epimerase, a putative membrane protein with sugar-modifying moiety, in a reverse orientation to EPS biosynthesis gene cluster. (ppjonline.org)

Mitochondria import many hundreds of different proteins that are encoded by nuclear genes. (nih.gov)

This gene was tagged by insertion of the Tc1 transposon, and the wild-type gene was cloned by chromosomal walking 11 kb from ama-1, a cloned gene encoding the large subunit of RNA polymerase II. (ncsu.edu)
Kask L., Hillarp A., Ramesh B., Dahlbäck B., and Blom A. M. (2002) Structural requirements for the intra-cellular subunit polymerization of the complement inhibitor C4b-binding protein. (lu.se)

Like group A Streptococcus, many pathogenic bacteria have completely sequenced genomes, but we don't know what most of the genes are doing. (scienceblog.com)
Interestingly there is one additional phage-encoded protein, pX (p10), that is important for regulating the number of double stranded genomes in the bacterial host. (bionity.com)

Further analysis showed, that Tn917-lac had inserted into actA, the second gene of an operon. (wikipedia.org)
The third gene of this operon, plcB, encodes the L. monocytogenes lecithinase. (wikipedia.org)
The nucleotide sequence revealed that glpF is the promoter-proximal gene of the glpFK operon. (nau.edu)
Two ORFs, orfX and orfY, encoding a putative regulatory protein and a carbohydrate kinase of unknown function, were located upstream of the glpFK operon. (nau.edu)
Certainly, STAS domain-encoding SulP genes have already been found as the different parts of antibiotic operons, like the BLM bleomycin biosynthesis operon of (Du et al. (exposed-skin-care.net)

The gene SLC39A4 was found to encode a solute carrier protein called human zinc/iron-regulated transporterlike protein (hZIP4). (medscape.com)
Solute transport on the sub 100 ms scale across the lipid bilayer membrane of individual proteoliposomes. (lu.se)

An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and recombinant phage carrying both genes ompAI and ompAII) selected by immunoscreening. (shengsci.com)
NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express. (neb.com)
The two most common problems during recombinant protein expression are low expression level and solubility of the target protein. (neb.com)
The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. (tno.nl)
Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. (lu.se)

alters an outer membrane protein which prevents the drug getting into the bacterial cell and accessing its intracellular target. (futurelearn.com)
[ 4 ] Protein hZIP4 transports zinc ions from the cell exterior or lumen of intracellular organelles into the cytoplasm, where it is available to other newly synthesized proteins. (medscape.com)

Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. (shengsci.com)
The encoded proteins (Mr = 33,564 and 32,536 for mature OmpAI and OmpAII, respectively) had only 64% identity with each other and otherwise had the highest level of homology to OmpA proteins from the members of the family Enterobacteriaceae. (shengsci.com)
by PCR revealed that possession of two tandem ompA genes was widespread among this genus. (shengsci.com)

ABSTRACT The emergence and rapid spread of antibiotic-resistant Klebsiella pneumoniae isolates harbouring the blaKPC gene that encodes for carbapenemase production have complicated the management of patient infections. (who.int)
Asthma is a reversible airway obstruction that is characterized by constriction of airway smooth muscle, Abstract hyper secretion of mucus, edema and airway hyper responsiveness (AHR), mucus secretion and thickening of the basement membrane underlying the airway epithelium. (cdc.gov)

Meningitis-causing bacteria exerted strong evolutionary pressure on an iron-binding protein in primates, a study shows. (the-scientist.com)
Cholinesterase-like domains in enzymes and structural proteins: functional and evolutionary relationships and identification of a catalytically essential aspartic acid. (expasy.org)
It also highlights the importance of HGT in the evolutionary history of CHS and describes bacterial chs genes for the first time. (biomedcentral.com)

Cytosine methylation has been studied in wheat rRNA genes at nucleolar organizers displaying different activities. (ncsu.edu)
The methylation pattern within a specific multigene locus is influenced by the number and type of rRNA genes in other rDNA loci in the cell. (ncsu.edu)
It is concluded that cytosine methylation in rDNA is regulated and that the methylation pattern correlates with the transcription potential of an rRNA gene. (ncsu.edu)
Discussion In the current study, we determined the methylation status of the SPARC gene promoter in pancreatic cancer cell lines, pancreatic cancer and corresponding adjacent normal pancreatic tissues, chronic pancreatitis tissues, and real normal pancreatic tissues. (hdacassay.com)
Methylation of the SPARC gene TRR gradually increased from normal, chronic pancreatitis, and the adjacent normal tissues to pancreatic cancer tissues. (hdacassay.com)
The methylation pattern of the SPARC gene TRR exhibited two hypermethylation wave peak regions: CpG Region 1 (CpG site 1-7) and CpG Region 2 (CpG site 8-12). (hdacassay.com)

These proteins are targeted to the mitochondria, translocated through the mitochondrial membranes, and sorted to the different mitochondrial subcompartments. (nih.gov)
Separate translocases in the mitochondrial outer membrane (TOM complex) and in the inner membrane (TIM complex) facilitate recognition of preproteins and transport across the two membranes. (nih.gov)

The targets of quinolone activity are the bacterial DNA gyrase and topoisomerase IV, enzymes essential for DNA replication and transcription. (cdc.gov)
to a membrane-scaffolded organic of fatty acidity biosynthetic enzymes (Babu et al. (exposed-skin-care.net)
Recently, bacterial glycine betaine synthesizing enzymes have become a major target in developing stress tolerant crop plants of economic interest. (scialert.net)
KPCs) are Ambler class A plasmid- gene by polymerase chain reaction cin, imipenem, ertapenem, meropenem, encoded enzymes that are capable of (PCR) assay provides laboratories trimethoprim/sulfamethoxazole, piper- hydrolyzing all beta-lactam antibiotics, with a means to quickly identify the acillin, piperacillin/tazobactam and including monobactams, extended- presence of this important resistance tobramycin (Oxoid). (who.int)

Here we explore the feasibility of extracting bacterial protein signals relevant to CD, by interrogating myriads of intestinal bacterial proteomes from a small number of patients and healthy controls. (bmj.com)
Results Our 2D-DIGE-based discovery approach revealed an imbalance of intestinal bacterial functions in CD. (bmj.com)
To date, only one bacterial factor has been shown to mediate intestinal adherence by O157:H7, the outer membrane protein intimin which was originally discovered in the Principal Investigator's laboratory. (usda.gov)

Open up in another window Physique 2 Structural topology style of SLC26 polypeptides displaying the brief cytoplasmic N-terminal area accompanied by a transmembrane domain name with 12 putative membrane-spanning -helices, as well as the C-terminal cytoplasmic area, largely composed of the STAS domain name. (exposed-skin-care.net)

Analysis of secondary metabolite gene and protein expression profiles in Streptomyces coelicolor grown under environmental conditions. (swan.ac.uk)
Here we describe our studies on the genetic organization of phages infecting Staphylococcus aureus, a pathogen of human importance, by using an assembly of tools for gene annotation, identification of expression components, and phylogeny analysis. (bvsalud.org)
Thanks for holding, and welcome to the New England BioLabs Solutions for the Expression of Difficult Proteins conference call. (neb.com)
Welcome to everyone who has chosen to join us for this information session on solutions for the expression of difficult proteins. (neb.com)
As a solution NEB offers a tunable T7 expression strain for the production of membrane proteins and other difficult targets so that the level of functional protein may be optimized. (neb.com)
First let me address the problem of low target protein expression. (neb.com)
When designing expression constructs, make sure that the vector does indeed encode a properly spaced ribosome binding site and also analyze for secondary structure at the five prime end of the messenger RNA. (neb.com)
Finally, low expression may be due to uncontrolled expression of toxic protein during the culture outgrowth phase. (neb.com)
The geminivirus transient expression system allows rapid analysis of RNAs transcribed from foreign genes and can serve as a preliminary screen in the construction of transgenic plants. (ncsu.edu)
Chromatin structure and expression of plant ribosomal RNA genes. (ncsu.edu)
Upon detection of environmental alterations, bacteria adjust their gene expression in order to obtain a phenotype that is suitable for the environment to which they are exposed ( Federle, 2012 ). (frontiersin.org)
Transcriptional regulators enable the microbes to sense and respond to environmental stimuli, by altering gene expression. (frontiersin.org)
Community level coordination of gene expression is known as quorum sensing (QS). (frontiersin.org)
After sequencing the RNAs expressed in normal and enlarged spheroplasts, we compared the gene expression levels of the 1100 orthologs in E. litoralis and L. amnigena . (aimspress.com)
Our findings indicate that spheroplast enlargement varies between different organisms with respect to gene expression. (aimspress.com)
This DNA then serves as a template for expression of the phage genes. (bionity.com)
If expression of a trait requires only one copy of a gene (one allele). (msdmanuals.com)
From experimentally validated target genes of these 86 miRNAs, pan-sensitive and pan-resistant genes with concordant mRNA and protein expression associated with in-vitro drug response to 19 NCCN-recommended breast cancer drugs were selected. (cdc.gov)

Bioinformatics studies also revealed the existence of genes coding for homologs of CTD. (nih.gov)

How bacterial cyclases recognize phage infection is not known. (bvsalud.org)
Despite phage replication in these matrices, lysis of the bacteria was slower than phage propagation, and no reduction of the bacterial population was observed. (bvsalud.org)
Consistent with these in vitro findings, phage treatment did not reduce bacterial burdens in a neutropenic mouse S. aureus thigh infection model. (bvsalud.org)
There are four other proteins on the phage surface, two of which have been extensively studied. (bionity.com)
At the other end of the phage particle are five copies of the surface exposed pIII (p3) and its less exposed accessory protein, pVI (p6). (bionity.com)
p3 is also the last point of contact with the host as new phage bud from the bacterial surface. (bionity.com)
pV (aka p5) competes with double stranded DNA formation by sequestering copies of the + stranded DNA into a protein/DNA complex destined for packaging into new phage particles. (bionity.com)

The primary proteinous product of the actA gene consists of 639 amino acids and includes the signal peptide (first N-terminal 29 amino acids) and the ActA chain (C-terminal 610 amino acids). (wikipedia.org)
Therefore, the sequence of the mature ActA protein consist of 610 amino acids. (wikipedia.org)
Spectinomycin and tetracycline prevent proteins from being assembled by the cell and streptomycin induces the assembly of the wrong amino acids into the translated protein. (icr.org)
Because they are so small - fewer than 50 amino acids in length - it's likely the proteins fold into unique shapes that represent previously unidentified biological building blocks. (technologynetworks.com)
These proteins are very small, containing only 33 and 32 amino acids respectively, though some additional residues can be added to the N-terminal portion of each which are then presented on the outside of the coat. (bionity.com)

Functional analysis of a novel DNA binding protein of Streptomyces coelicolor. (swan.ac.uk)
4] Failure of DNA to properly separate during these processes results in a bacterium not being able to divide normally or produce functional proteins. (icr.org)
When expressing membrane proteins, researchers must assume from the start that the protein will be difficult to express in functional form. (neb.com)
Spiller B. O., Blackbourne, D., Mark L, Proctor D. and * Blom A. M. , (2003) Functional activity of the complement regulator encoded by Kaposi's sarcoma associated herpesvirus. (lu.se)

For example, inactivation of genes encoding for subunits of the ATP synthase, atpA , atpB , atpG and atpH , reduced the minimum inhibitory concentration (MIC) of gentamicin 16-fold. (frontiersin.org)
All such systems are multisubunit complexes with at least 3 dissimilar subunits embedded as a complex in the membrane (F 0 , a:b:c = 1:2:~12) and (usually) at least 5 dissimilar subunits attached to F 0 (F 1 , α:β:γ:δ:ε = 3:3:1:1:1 for F-type ATPases). (tcdb.org)

IMPORTANCE: Conjugation is a major form of horizontal gene transfer and has played a profound role in bacterial evolution by moving genes, including those involved in antibiotic resistance, metabolism, symbiosis, and infectious disease. (bvsalud.org)
A second protein, glycerol kinase, is involved in entry of external glycerol into cellular metabolism by trapping glycerol in the cytoplasm as sn-glycerol 3-phosphate. (nau.edu)

Recently, darobactin A, a novel bicyclic heptapeptide that selectively kills Gram-negative bacteria by targeting the outer-membrane protein BamA, was discovered. (sdsc.edu)
The most potent compound, darobactin B, was used for co-crystallization with the target BamA, revealing an identical binding site to darobactin A. Besides its potency, darobactin B did not exhibit cytotoxicity and was slightly more active against Acinetobacter baumanii isolates than darobactin A. Furthermore, we evaluated the plasma protein binding of darobactin A and B, indicating their different pharmacokinetic properties. (sdsc.edu)

The Actin assembly-inducing protein (ActA) is a protein encoded and used by Listeria monocytogenes to propel itself through a mammalian host cell. (wikipedia.org)
In a mammalian cell the bacterial ActA interacts with the Arp2/3 complex and actin monomers to induce actin polymerization on the bacterial surface generating an actin comet tail. (wikipedia.org)
NPFs in the mammalian cell recruit and bind to the already existing actin-related-protein 2 and 3 complex (Arp2/3 complex) and induce an activating conformational change of the Arp2/3 complex. (wikipedia.org)
2010). The mammalian and bacterial STAS domains reported to day have already been monomeric in answer. (exposed-skin-care.net)

Most variation between the two proteins was localized to the predicted surface loops and periplasmic turns, while the transmembrane strands and C-terminals domains were highly conserved. (shengsci.com)
In natural deer populations an allelic variation in the prion gene is thought to confer resistance to CWD. (carroll.edu)

Efflux pumps, located in the cell membrane, are one method of protection that many bacteria use against the influx of antibiotics. (icr.org)

[ 3 ] This protein controls zinc uptake across the plasma membrane of various cell types, including the intestine. (medscape.com)
HvPIP1;6, a Barley (Hordeum vulgare L.) Plasma Membrane Water Channel Particularly Expressed in Growing Compared with Non-Growing Leaf Tissues. (lu.se)

Of more concern is resistance acquired via a mobile genetic element (e.g. plasmids), as in this case multiple antibiotic resistance genes maybe present on the same element, rendering the bacteria resistant to multiple classes of drug. (futurelearn.com)
Is this resistance the result of bacteria evolving new genes in response to the presence of antibiotics, or are antibiotic-resistant bacteria selected for in the environment by possessing antibiotic resistance genes beforehand? (icr.org)
To answer these questions a discussion of several factors involved in antibiotic resistance will show that resistance is a designed feature of pre-existing genes enabling bacteria to compete with the antibiotic producers in their environment. (icr.org)
The increase in resistance among these organisms clearly indicates a change in the frequency of antibiotic resistance genes. (icr.org)
The emergence of antimicrobial resistance severely threatens our ability to treat bacterial infections. (frontiersin.org)
Gene products that confer intrinsic resistance to antimicrobial agents may be explored for alternative antimicrobial therapies, by potentiating the efficacy of existing antimicrobials. (frontiersin.org)
Our results demonstrate that many gene products contribute to the intrinsic antimicrobial resistance of S. aureus . (frontiersin.org)

Indeed, manifestation of SulP genes Rv1739c and Rv1707 was upregulated in triggered macrophages 24 hrs post-infection, therefore may are likely involved in mycobacterial success, latency, or pathogenesis in hypoxic BIIE 0246 supplier or additional stress circumstances. (exposed-skin-care.net)

dpy-13: A nematode collagen gene that affects body shape. (ncsu.edu)
The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. (tno.nl)

Aquaporin-9 Protein Is the Primary Route of Hepatocyte Glycerol Uptake for Glycerol Gluconeogenesis in Mice. (lu.se)

A mutation in this gene causes decreased zinc secretion. (medscape.com)

Antibiotics are 'magic bullets' with the aim of targeting parts of the bacterial physiology that are not present in eukaryotic cells. (futurelearn.com)
The SPI-1 and SPI-2 genes code for proteins forming the type III secretion system (T3SS) which enable the transport of S. enterica proteins from the bacterial cell directly into the cytosol of eukaryotic cells. (biomedcentral.com)

A fusion of ConE to green fluorescent protein (GFP) localizes to the membrane preferentially at the cell poles. (bvsalud.org)
One CCGG site 164 base-pairs upstream from the start of transcription is preferentially unmethylated in some genes. (ncsu.edu)

Prion proteins are concentrated to these lipid raft regions. (carroll.edu)
Zinc stabilizes cell membranes by reducing free radicals and preventing lipid peroxidation. (medscape.com)
Structure and Stability of the Spinach Aquaporin SoPIP2;1 in Detergent Micelles and Lipid Membranes. (lu.se)

Using an adenylate cyclase-based bacterial two-hybrid system, we found that ConE interacts with ConB. (bvsalud.org)
We propose a model in which the ICEBs1 conjugation machinery is composed of ConB, ConC, ConD, ConE, ConG, CwlT, ConQ, and possibly other ICEBs1 proteins, and that ConB interacts with ConE, helping to recruit and/or maintain ConE at the membrane. (bvsalud.org)

This study focused on characterization of four SEDS genes [SC02085 (ftsW), SC02607 (sfr), SC03846 (rodA) and SC05302 (rodA2)] and SC02090 (ftsi) and SC02608 ipbp2) [cognate HMW PBP genes ftsWand sfr, respectively]. (swan.ac.uk)
The candidate will be involved in the characterization of essential proteins involved in cell envelope biogenesis in the human pathogen Pseudomonas aeruginosa and in the evaluation of inhibitors identified by virtual screening through in vitro and in vivo assays. (simgbm.it)
Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. (lu.se)

In addition, the enlarged spheroplasts of L. amnigena , which differ in the size of inner and outer membranes, were larger than those of E. litoralis . (aimspress.com)

Moreover, although the abundance of most protein groups reflected that of related bacterial populations, we found a specific independent regulation of bacteria-derived cell envelope proteins. (bmj.com)

The majority of the identified genes have not previously been associated with antimicrobial susceptibility in S. aureus . (frontiersin.org)
The helper-drug can target gene products that by any mechanism aid bacteria to resist higher concentrations of an antimicrobial ( Pieren and Tigges, 2012 ). (frontiersin.org)
that large and complex networks of both established and yet uncharacterized gene products contribute to reduce the inhibitory activity of antimicrobial agents. (frontiersin.org)

Throughout the cell cycle, the chromosome progressively goes from being fully methylated to hemimethylated during DNA replication - this results in differential binding of regulatory proteins to activate or repress transcription. (kenyon.edu)
The responsible gene has been localized on the long arm of chromosome 7. (msdmanuals.com)

The genes, subcutaneous fitness genes A (scfA) and B (scfB), may prove to be promising clinical targets in the fight against these infections, as there are no vaccines against group A Streptococcus or effective treatments for invasive infections. (scienceblog.com)
These genes would be good targets for a vaccine or treatment because the bacteria missing these genes did not flourish in the infection site. (scienceblog.com)
The results suggest that these two genes are involved in the invasion process and may be potential targets for therapeutics. (scienceblog.com)

For these reasons we chose to prepare them biosynthetically, through exploitation of the action of another recently identified bacterial glycosyltrasferase known as dolichol-phospho-beta-d-mannose synthase (DPMS). (europa.eu)

The gene SC6D7.16 was cloned from the DNA of s. coelicolor A(3)2 and, from it, the DPMS was successfully expressed in soluble form in e. (europa.eu)
Recently, the characteristics of a family of soluble carotenoid-binding proteins (Helical Carotenoid Proteins [HCPs]), paralogs of the N-terminal domain of OCP, were described. (nih.gov)

A chromosomal ΔglpFK mutant was isolated by gene replacement. (nau.edu)

Bacteriophage transduction is the major mechanism of horizontal gene transfer (HGT) among many bacteria. (bvsalud.org)

A small amount of bacterial SulP transporters absence a C-terminal STAS domain name, but have in its place an enzymatically energetic (Nishimori et al. (exposed-skin-care.net)

In parallel, we used 16S rRNA gene sequencing for an integrated analysis of gut ecosystem structure and functions. (bmj.com)

This was studied by using the CtrA gene, which encodes for an important cell cycle regulatory protein. (kenyon.edu)
Blom, A. M. , Rytkönen, A., Vasquez, P., Dahlbäck, B. and Johnsson, A-B. (2001) A novel interaction between type IV pili of Neisseria gonorrhoeae and complement regulatory protein - C4b-binding protein. (lu.se)

Anatomy41

Organisms16

Diseases1

Chemicals and Drugs102

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes62

Disciplines and Occupations1

Technology, Industry, Agriculture7

Information Science4

Health Care1

Virulence6

Major outer membrane protein2

Mutations9

Cytoplasmic membrane3

Synthesis7

Escherichia9

Strains6

Enzyme9

Genome13

Sequences6

Pathogens6

Coli10

Signal transduction1

Mucous membranes2

Effector proteins1

Receptors1

Species6

Molecular10

Polypeptide5

Mutants6

Nuclear genes1

Subunit2

Genomes2

Operon5

Solute2

Recombinant5

Intracellular2

OmpA3

Abstract2

Evolutionary3

Methylation6

Mitochondrial2

Enzymes4

Intestinal3

Putative1

Expression18

Homologs1

Phage7

Amino5

Functional4

Subunits2

Metabolism2

BamA2

Mammalian4

Variation2

Efflux1

Plasma membrane2

Resistance7

Pathogenesis1

Collagen2

Uptake1

Mutation1

Eukaryotic cells2

Preferentially2

Lipid3

Interacts2

Characterization3

Inner and outer1

Cell envelope1

Antimicrobial3

Chromosome2

Targets3

Synthase1

Soluble2

Chromosomal1

Horizontal gene tra1

Transporters1

RRNA gene1

Regulatory protein2