• generate
  • However, because they are already epigenetically programmed to favor a particular fate, they generally cannot generate cell types outside of their lineage without genetic manipulation. (conservapedia.com)
  • As scientists in the field devise better ways to generate these cells (new techniques are a current area of much research--there have been at least 40 published since 2009), they may become more clinically useful. (conservapedia.com)
  • Formation
  • Culture of mouse ES cells as embryoid bodies (EBs) has provided a convenient system for studying early mouse developmental processes, including several aspects of extraembryonic lineage and axis formation associated with the pre‐ and peri‐gastrulating mouse embryo. (currentprotocols.com)
  • maintain
  • Mouse ES cells maintain an undifferentiated state through serial passages when cultured in the presence of fibroblast feeder layers in the presence of Leukemia Inhibitory Factor (LIF) (Williams, et al. (google.es)
  • mouse
  • Furthermore, derivation of mouse ES cells has allowed for the generation of thousands of gene‐targeted mouse mutants. (currentprotocols.com)
  • One of the seminal achievements of mammalian embryology of the last decade is the routine insertion of specific genes into the mouse genome through the use of mouse ES cells. (google.es)
  • field
  • Understanding the mechanisms underlying the regulation of these stem cells has led to many recent breakthroughs in the field of cancer biology. (conservapedia.com)