• Expressed Sequence Tags and isolated cDNA clones indicate KIAA0090 is expressed ubiquitously in low to moderate levels throughout the body. (wikipedia.org)
  • From a cDNA library of 3-day after-pollination (DAP) rice embryos we cloned a HAZ1 cDNA that encodes a protein with a PHD-finger domain and a homeodomain. (elsevierpure.com)
  • cDNA of heavy chain (HC) and light chain (LC) of mAb BR55-2 (9) were cloned from the hybridoma-producing murine IgG2a LeY oligosaccharide-specific antibody and placed into the pBI121 binary vector (Clontech), yielding pRB59-2 (Fig. 1Ti plasmid, respectively) was transferred into tobacco genomic DNA by and and and Cytotoxicity of mAbP BR55-2. (researchensemble.com)
  • The δN- XProfilin2 cDNA lacking the XProfilin2 MO recognition sequence is insensitive to the effects of the XProfilin2 MO. (xenbase.org)
  • An integrated framework for cDNA library production, sequencing, quality control, expression data generation, and systems-level analysis is presented and utilized. (biomedcentral.com)
  • a loblolly pine (pinus taeda l.) cdna with properties of a nonspecific lipid transfer protein (nsltp) is reported. (liverpool.ac.uk)
  • two genes preferentially expressed in differentiating xylem of loblolly pine (pinus taeda l.) were cloned from cdna and genomic libraries and designated ptx3h6 and ptx14a9. (liverpool.ac.uk)
  • From cDNA libraries, we isolated a 345 bp cDNA clone corresponding to the C53A.2 gene assigned on WormBase. (wormbook.org)
  • RNA interference experiments using this cDNA sequence reproduced the Ven phenotype. (wormbook.org)
  • in which the 472 bp upstream region of the ven-1 open reading frame was fused to green fluorescent protein (GFP) cDNA (Figure 2A). (wormbook.org)
  • Sequence analysis revealed that cDNA of VlRR5 was 2 597 bp in length,encoding 693 amino acids,and located on chromosome 4 of grape. (ahs.ac.cn)
  • In order to ascertain the function of sucrose-phosphate synthase genes( SPS )in the regulation of sweet cherry ripening,four full-length cDNA of sweet cherry SPS genes( PavSPSA1 , PavSPSA2 , PavSPSB ,and PavSPSC )were cloned in sweet cherry genome. (ahs.ac.cn)
  • Cas9-based genome editing to endogenously tag Osk with the decapping complex recruitment leads to turnover of select maternal mRNAs. (billfryer.com)
  • Whole genome sequencing was conducted using Illumina HiSeq X ten at WuXi AppTec. (biomedcentral.com)
  • Standard techniques like cell synchronization by serum starvation, flow cytometry, N-terminal or C-terminal FLAG epitope-tagged protein expression, immunofluorescence confocal microscopy, dual luciferase assay for promoter evaluation, and chromatin immunoprecipitation assay were employed during this study. (biomedcentral.com)
  • The six Arabidopsis ACBPs are subcellularly located in various compartments based on experimental results using a variety of techniques including fluorescent protein tagging in confocal microscopy, immunoelectron microscopy and western blot analysis of subcellular fractions from wild-type plants as well as from transgenic plants expressing fluorescent-tagged ACBPs. (aocs.org)
  • In the vertebrate CNS, HB9/MNR2 transcription factors are specifically expressed in all somatic motoneurons and are necessary to distinguish motoneurons from interneurons, in part by repressing interneuron-specific gene expression. (jneurosci.org)
  • Together, vertebrate HB9/MNR2 transcription factors are expressed in all somatic motoneurons and excluded from interneurons. (jneurosci.org)
  • Thus, vertebrate HB9/MNR2 genes are expressed specifically in somatic motoneurons and are essential for distinguishing motoneuron/interneuron cell types. (jneurosci.org)
  • Drosophila HB9 differs from its vertebrate orthologs in several ways: it is not expressed in all somatic motoneurons, it is expressed in a subset of interneurons, and it is required for the proper development of both interneurons and motoneurons. (jneurosci.org)
  • The study of amphibian embryogenesis has provided important insight into the mechanisms of vertebrate development. (biomedcentral.com)
  • Gastrulation is a critical morphogenetic event during vertebrate embryogenesis, and it is comprised of directional cell movement resulting from the polarization and reorganization of the actin cytoskeleton . (xenbase.org)
  • We evaluated FOXP1 activity by an in vitro luciferase model and assessed protein stability in vitro by western blotting. (bmj.com)
  • Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
  • The function of the acyl-CoA-binding domain in recombinant Arabidopsis ACBPs expressed in the bacterium Escherichia coli was established using site-directed mutagenesis followed by in vitro binding assays. (aocs.org)
  • 2008. A plant virus movement protein forms ringlike complexes with the major nucleolar protein, fibrillarin, in vitro . (hutton.ac.uk)
  • Complex patterns of posttranslational modifications of chromatin including acetylation of histones establish combinatorial signals that are apparently interpreted by chromatin-binding proteins and the gene expression machinery to control transcriptional activity of individual genes. (aacrjournals.org)
  • DEAF-1 recognizes several TTCG motifs within the portion of the Dfd autoregulatory region termed 'module E.' In addition, DEAF-1 binds several similar motifs within a Dfd response element ( DRE ) from the 1.28 gene that enhances maxillary gene expression during embryogenesis (Pederson, 2000). (sdbonline.org)
  • Gene expression analysis showed that the expression levels of PavSPSA1 was the highest,followed by PavSPSC ,whereas PavSPSA2 and PavSPSB were relatively weakly expressed during the fruit growth and development,and ripening using quantitative real-time PCR. (ahs.ac.cn)
  • The results showed that the expression of different types of proteins change in different characteristics in salivary glands during the unfed to engorged process of female ticks. (biomedcentral.com)
  • Sanger sequencing chromatograms of single- and double-mutant chromatograms. (billfryer.com)
  • In situ hybridization showed that HAZ1 was expressed at a higher level in the outer layers of a developing embryo than in the inner parts of the embryo at 3 DAP. (elsevierpure.com)
  • To start with, we have been interested in whether or not n4bp3 is expressed within the establishing nervous system of X. laevis and investigated its expression profile in the course of early embryogenesis by full mount in situ hybridization experiments using a specific antisense n4bp3 RNA probe. (cret-signal.com)
  • In plants cells, lipid-transfer proteins (LTPs) and acyl-CoA-binding proteins (ACBPs) have been identified as candidates for lipid transfer within the cell [reviewed in 1,2]. (aocs.org)
  • lipid transfer protein genes of loblolly pine are members of a complex gene family. (liverpool.ac.uk)
  • Salt stress in Arabidopsis: lipid transfer protein AZI1 and its control by mitogen-activated protein kinase MPK3. (uni-bielefeld.de)
  • A wheat lipid transfer protein 3 could enhance the basal thermotolerance and oxidative stress resistance of Arabidopsis. (uni-bielefeld.de)
  • Engineered resonance energy transfer (RET) between a mTurquoise2 fluorescent protein and FMN-bound As LOV2 increases switch sensitivity. (nature.com)
  • This generalises to other fluorescent protein tags and As LOV2-based actuators. (nature.com)
  • Analysis indicates the KIAA0090 unspliced protein product to be 993 amino acids long with an isoelectric point of 7.418 and a molecular weight of 111765.73 Daltons. (wikipedia.org)
  • The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
  • Furthermore, to the best of our knowledge, there are only a few reports on the role of molecular motor and TCA cycle-related proteins in the salivary glands of ticks. (biomedcentral.com)
  • Using bioinformatics methods to analyze the dynamic changes of a large number of proteins, we found that molecular motor and TCA cycle-related proteins play an important role in the physiological changes of the salivary glands. (biomedcentral.com)
  • By analyzing these changes, some proteins, such as molecular motor and TCA cycle-related proteins, were screened and RNAi carried out. (biomedcentral.com)
  • These transcript tags were annotated with gene ontology (GO) terms. (biomedcentral.com)
  • One of the most striking results from the expression analysis was that the drought sensitive plants were characterised by having approximately twice the number of differentially expressed transcript tags than the tolerant plants after drought. (biomedcentral.com)
  • However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. (origene.com)
  • The ven-1 transcript encoded a polypeptide of 86 amino acid residues, including the signal sequence at the N-terminus, suggesting that VEN-1 is a secreted protein (Figure 1B). (wormbook.org)
  • To examine the possibility of VEN-1 being a secreted protein, we tested whether forced expression of the ven-1 transcript under the control of heterologous promoters could rescue the Ven phenotype in tissues. (wormbook.org)
  • We also examined the temporal requirement of ven-1 activity by expressing the transcript using a heat shock promoter. (wormbook.org)
  • Dependent on splice outcome and posttranslational modification, these additional signals indicate the protein could localize to the peroxisome, the plasma membrane, outside the cell, the cytosol, the nucleus, or mitochondria. (wikipedia.org)
  • The N -terminal transmembrane domain of ACBP1 and ACBP2 targets each protein to the endoplasmic reticulum (ER) and the plasma membrane while ACBP3 is targeted extracellularly. (aocs.org)
  • This study provides the first in vivo evidence of trapped SD proteins in clathrin-coated pits at the plasma membrane when this pathway is disrupted. (sdbonline.org)
  • Some proteins are transported to lysosomes, other internalized proteins are delivered to the trans-Golgi network or back to the plasma membrane by recycling endosomes. (biomedcentral.com)
  • In the Xenopus embryo , XProfilin2 is temporally expressed throughout embryogenesis and it is spatially expressed in cells undergoing morphogenetic movement during gastrulation. (xenbase.org)
  • pqbp1 and wbp11 genes are similarly expressed in mesoderm and neural tissues during Xenopus development. (xenbase.org)
  • G) Translation of C-terminal myc -tagged Xenopus PQBP1 (PQ- myc ) was blocked by co-injection of pqbp1 MO1 but not control MO (CT). (xenbase.org)
  • Transgenic frogs that express the enzyme in the germline can be bred with animals harboring a transposon substrate to generate double transgenic lines where remobilization will occur in the germline in subsequent generations. (biomedcentral.com)
  • Results Generation of Transgenic Plants Expressing Lewis Y-Specific mAb BR55-2. (researchensemble.com)
  • Knockdown of Nedd4 binding protein three in producing primary hippocampal cultures alters axonal and dendritic branching Over the basis of our localization research, we cloned a functional N4BP3 interfering RNA con struct and performed transient N4BP3 KD experiments in main hippocampal neurons throughout the periods of axonal and dendritic development. (cret-signal.com)
  • Mouse HB9 maintains expression in somatic motoneurons, whereas chick HB9 is expressed only in postmitotic somatic motoneurons. (jneurosci.org)
  • This clone was engineered to express the complete ORF with an expression tag. (origene.com)
  • In response to a brain injury, astrocytes proliferate and become hypertrophic with an increased expression of intermediate filament proteins. (jneurosci.org)
  • Forced expression of lcn2 or treatment with LCN2 protein increased the sensitivity of astrocytes to cytotoxic stimuli. (jneurosci.org)
  • In zebrafishes, forced expression of rat lcn2 gene increased the number and thickness of cellular processes in GFAP-expressing radial glia cells, suggesting that lcn2 expression in glia cells plays an important role in vivo . (jneurosci.org)
  • In this process, astrocytes proliferate to fill gaps and undergo a typical morphological change: a large cytoplasmic mass, long and branched processes, and increased expression of intermediate filaments such as glial fibrillary acidic protein (GFAP). (jneurosci.org)
  • The expression levels of a large number of proteins during the development and degeneration of salivary glands change, which regulate the biological functions of the salivary glands. (biomedcentral.com)
  • Corresponding expression changes of these proteins at different developmental stages of female ticks are very important to ensure the orderly development of the organ. (biomedcentral.com)
  • A L-R expression screen identified the cytoskeletally-associated gene, actin binding lim protein 1 ( Ablim1 ), as asymmetrically expressed in both the node and left lateral plate mesoderm (LPM). (biomedcentral.com)
  • These results where can you get clarinex suggest that PAM-distal cleavage by Cas12a To investigate the effect on pole cell expressing endogenously tagged Osk-sfGFP or Vas-EGFP (Fig 3B) to account for loss of nos and pgc RNA detected pairwise by smFISH in granules in pre-pole bud stage embryos. (billfryer.com)
  • The kinetic parameters of recombinant proteins showed that ZmHXK4-7 have high catalytic efficiency for Glc, fructose (Fru) and mannose (Man), ZmHXK7 has a lower Km for ATP, and together with ZmHXK8 they have lower sensitivity to inhibition by ADP, G6P and N-acetylglucosamine than ZmHXK4-6 and ZmHXK9. (biomedcentral.com)
  • Here, we cloned and functionally characterized putative catalytic enzymes to analyze their metabolic contribution during germination process. (biomedcentral.com)
  • These mice express a catalytically inactive fusion protein of the NH 2 -terminal part of HDAC2 and β-galactosidase, which fails to integrate into corepressor complexes with mSin3B. (aacrjournals.org)
  • The mAb fusion to the KDEL signal sequence helped to retain the protein inside the endoplasmic reticulum (ER), thus enhancing mAb assembly in plant cells (17). (researchensemble.com)
  • The embryonic AMPs are at the origin of all adult fly muscles and, as we demonstrate here, they express repressors of myogenic differentiation and targets of the Notch pathway known to be involved in muscle cell stemness. (biologists.com)
  • Both genes are expressed in the nascent mesoderm and neurectoderm , and morpholino knockdown of either causes defects in differentiation and morphogenesis of the mesoderm and neural plate . (xenbase.org)
  • specifically the centromeric protein CENPH, the BAX Inhibitor TMBI4, the ADP ribosylation factor ARF6, the kinase TNIK, and the transcriptional repressor T22D1. (wikipedia.org)
  • Following recognition of TLR ligands, TLRs elicit innate immunity by activating multiple intracellular signaling cascades including variable adaptor proteins and transcriptional factors. (frontiersin.org)
  • the gene encoding the monolignol biosynthetic enzyme cinnamyl alcohol dehydrogenase (cad, e.c. 1.1.1.195) can be expressed in response to different developmental and environmental cues. (liverpool.ac.uk)
  • DEAF-1 is maternally expressed, and the encoded protein is broadly distributed throughout the early embryo. (sdbonline.org)
  • Using WISH during zebrafish embryogenesis, we demonstrated that vps50 gene was expressed throughout the early embryo, especially in the head. (biomedcentral.com)
  • We describe a bioinformatics pipeline known as FunnyBase that has been used to store, annotate, and analyze 40,363 expressed sequence tags (ESTs) from the heart and liver of the fish, Fundulus heteroclitus . (biomedcentral.com)
  • To assess the role of an individual HDAC isoenzyme in physiology and tumor development, HDAC2-mutant mice were generated from a gene trap embryonic stem cell clone. (aacrjournals.org)
  • Disrupting any component in this pathway led to disrupted SD on the cell surface and intracellular accumulation of mislocalized SD proteins. (sdbonline.org)
  • This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
  • in which the C-terminus of VEN-1 in the rescue fragment was tagged with an influenza hemagglutinin (HA) peptide. (wormbook.org)
  • KIAA0090 interacts with multiple effector proteins. (wikipedia.org)
  • We had previously identified the Formin protein Daam1 and an effector molecule XProfilin1 as links for Wnt-mediated cytoskeletal changes during gastrulation. (xenbase.org)
  • The mRNA for this gene codes for 18 protein isoforms6. (wikipedia.org)
  • The reverse activity transcribes the ZAR2 mRNA with 966 nt coding sequence which codes for a 321 amino acid protein. (biomedcentral.com)
  • ACBP1 mRNA is more highly expressed in seeds and siliques than vegetative organs. (aocs.org)
  • In vertebrates, chick MNR2 and mouse HB9 are expressed in presumptive somatic motoneuron progenitors. (jneurosci.org)
  • Partial alignment of Vps50 amino acid sequences between human and other vertebrates was aligned using the Clustalx program. (biomedcentral.com)
  • Our observations suggest that the HXKs may be redundant proteins with specific roles depending on carbon and ATP availability, metabolic needs, or sensor requirements. (biomedcentral.com)
  • Histone deacetylases (HDAC) reverse the acetylation of histone and nonhistone proteins and thereby modulate chromatin structure and function of nonhistone proteins. (aacrjournals.org)
  • While studying the activity of BRCA2 gene promoter in breast cancer cells, we discovered that this promoter has bi-directional activity and the product of the reverse activity (a ZAR1-like protein, we named ZAR2) silences the forward promoter at the G0/G1 phase of the cell. (biomedcentral.com)
  • The experiments showed that in the inactive form, the two ends of the protein form a sheet that closes a key site on the protein and prevents it from changing into its active form. (elifesciences.org)
  • However, the role of VPS50 in embryogenesis and human physiology are poorly understood. (biomedcentral.com)
  • The protein is highly conserved throughout Eukaryotes both in multi and single cellular organisms. (wikipedia.org)
  • The human cellular prion protein (PrP C ) is a glycosylphosphatidylinositol (GPI) anchored membrane glycoprotein with two N-glycosylation sites at residues 181 and 197. (mdpi.com)
  • ACBP4 and ACBP5 show conservation in a domain of five kelch motifs, which also enable interaction with protein partners. (aocs.org)
  • Other available systems, such as bacterial and yeast, do not provide specific machinery for protein posttranslational modifications required for an active or partially active mAb. (researchensemble.com)
  • Polyglutamine-binding protein 1 ( PQBP1 ) is an RNA-splicing factor that, when mutated, in humans causes Renpenning syndrome, an X-linked intellectual disability disease characterized by severe cognitive impairment, but also by physical defects that suggest PQBP1 has broader functions in embryonic development. (xenbase.org)
  • Under physiological conditions, LeY is expressed predominantly during embryogenesis but is restricted to granulocytes and epithelial surfaces in Lapatinib distributor adult tissue (10). (researchensemble.com)
  • ACBP mRNAs are expressed in all plant organs with some mRNAs more highly expressed than others in specific organs, e.g. (aocs.org)
  • In mammals, up to 10 diverse p63 isoforms exist with the longest one, TAp63α, being highly expressed in primary oocytes that are arrested in prophase of meiosis I. After homologous recombination, oocytes are kept in this dictyate arrest phase until they are recruited for ovulation, a period that can take decades in humans. (elifesciences.org)
  • Side-by-side single-cell RNA sequencing revealed similarities and differences in transcriptome profiles between pre-implantation blastoids and blastocysts, as well as post-implantation cultures, and uncovered a population resembling early migratory trophoblasts during co-culture with endometrial stromal cells. (bvsalud.org)
  • Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. (origene.com)
  • We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)
  • Thus, these results suggest that the SD proteins in human kidney undergo the same endocytosis and recycling pathway to maintain the filtration structure, and mutations in any genes in this pathway could lead to abnormal SD and renal diseases. (sdbonline.org)
  • KIAA0090 is a human gene coding for a protein of unknown function. (wikipedia.org)
  • Previously we successfully expressed the human rabies virus-neutralizing mAb SO57 (14) and the murine anticancer mAb C017-1A (15) activity similar to that of the parental mAb produced in the mammalian system. (researchensemble.com)
  • Single-cell RNA-sequencing unveiled transcriptomic similarities between advanced human peri-gastruloids and primary peri-gastrulation cell types found in humans and non-human primates. (bvsalud.org)
  • The research results provide a new protein target for the control of ticks and tick-borne diseases. (biomedcentral.com)
  • By binding to antigen expressed on the surface of cancer cells, mAbs trigger antibody-dependent cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity, which kills abnormal cells (3C5). (researchensemble.com)
  • In the comma stage, it was expressed in hypodermal cells: Abpl/raapppp (H7), P1/2 L/R, P3/4 L/R, P5/6 L/R, P7/8 L/R, P9/10 L/R, and P11/12. (wormbook.org)
  • sequence rescued the Ven phenotype, and the HA epitope was detected in hypodermal cells (Figure 2B). (wormbook.org)
  • Aux is expressed in multiple types of intestinal cells. (sdbonline.org)
  • Renowned as the #1 antibody event in the industry, this year's agenda boasts 15 dedicated topic streams, 3 unmissable training course add-on options and will bring together more than 700 of the antibody and protein community. (cshlpress.com)
  • Plants respond to various stimuli under abiotic or biotic stress condition and express certain genes either structural or regulatory genes which maintain the plant integrity. (scielo.br)
  • We used iTRAQ quantitative proteomics to study the dynamic changes in salivary gland proteins in female Haemaphysalis longicornis at four feeding stages: unfed, partially fed, semi-engorged and engorged. (biomedcentral.com)
  • In this study,a cytokinin response regulator VlRR5 was cloned from Kyoho grapevine,which has a phosphoric acid receptor domain(REC)and a MYB-like DNA binding domain. (ahs.ac.cn)