• Importantly, the cross-neutralizing antibody responses are focused towards conserved RBD epitopes outside of the ACE-2 receptor-binding motif. (biorxiv.org)
  • This may be an effective strategy for eliciting broadly neutralizing responses leading to a pan-sarbecovirus vaccine. (biorxiv.org)
  • Both IM and ID devices produced high-titer anti-SNV neutralizing antibody responses in rabbits and NHPs. (nih.gov)
  • Mice immunized with RBD-nanoparticles, but not soluble antigen, elicited cross-reactive antibody binding and neutralization responses, confirming increased immunogenicity from multimerization. (ox.ac.uk)
  • To address this issue, we have analyzed the kinetics of neutralizing antibody responses and their association with disease severity. (irsicaixa.es)
  • Plasma neutralizing responses were assessed against replicative virus and pseudoviral particles. (irsicaixa.es)
  • The ideal HIV vaccine would elicit humoral responses effective against a broad spectrum of primary HIV strains but immunogens capable of generating such antibodies in humans have been difficult to identify. (the-scientist.com)
  • Human papillomavirus 16L1-58L2 chimeric virus-like particles elicit durable neutralizing antibody responses against a broad-spectrum of human papillomavirus. (oncotarget.com)
  • His rational approach to structure-based vaccine design led to elucidation of broadly protective human immune responses to HIV, leading to the discovery of broadly neutralizing antibodies to the highly conserved CD4 binding site of HIV with his colleagues from the VRC. (wikipedia.org)
  • Interestingly, a leading ZIKV vaccine candidate elicited minimal BOB reactivity despite generating neutralising antibody responses . (bvsalud.org)
  • Immunization with recombinant EDIII protein effectively induced antigen-specific binding antibodies and cellular immune responses. (tghn.org)
  • The research evaluates SARS-CoV-2-specific antibody responses after vaccination of 288 healthcare workers with Sputnik V in Argentina, measuring IgG anti-spike titers and neutralizing capacity after one and two doses among non-infected and previously infected individuals. (healthofasia.com)
  • The vaccine was safe and well tolerated, induced potent serum IgG & IgA responses waning post last vaccination, and low and intermittent mucosal IgG, but did not induce neutralizing activity neither in serum nor vaginal samples. (europa.eu)
  • Although laboratory evidence suggests that antibody responses following COVID-19 vaccination provide better neutralization of some circulating variants than does natural infection ( 1 , 2 ), few real-world epidemiologic studies exist to support the benefit of vaccination for previously infected persons. (cdc.gov)
  • Adenoviruses are immunogenic and elicit strong innate and adaptive immune responses. (medscape.com)
  • Clinical data demonstrate that Moderna's proprietary vaccine technology has been generally well-tolerated and can elicit durable immune responses to viral antigens. (pharmiweb.com)
  • The Phase 3 study follows the interim Phase 2 results which showed that the adjuvanted recombinant COVID-19 vaccine candidate achieved high rates of neutralizing antibody responses in all adult age groups, with 95 to100% seroconversion rates. (gsk.com)
  • This concept of anti-idiotype antibodies mediating effects and limiting efficacy could have a profound impact in understanding how to increase effectiveness and duration of protective antibody responses," he explained. (medicalnewstoday.com)
  • The vast majority of research on both SARS-CoV-2 infection responses and vaccines solely centers on the protective antibody and T cell responses. (medicalnewstoday.com)
  • The animal models used to test immunogenicity and antibody responses to viral challenge included BALB/c mice, rhesus macaques, and Golden Syrian hamsters. (news-medical.net)
  • Furthermore, peripheral blood mononuclear cells (PBMCs) or splenocytes harvested from the vaccinated animals 14 days after the second vaccine dose and analyzed through flow cytometry were used for intracellular cytokine staining assay and enzyme-linked immunospot (ELISPOT) assay to examine the immune responses elicited by the vaccines. (news-medical.net)
  • Neutralizing antiviral B cell responses. (smw.ch)
  • The limited success achieved with induction of DENV-specific antibody responses suggests that inactivation of DENV may also require induction of CD8+ T lymphocytes (cell-mediated immunity). (virology.ws)
  • Allo-Specific Humoral Responses: New Methods for Screening Donor-Specific Antibody and Characterization of HLA-Specific Memory B Cells. (duke.edu)
  • The RBD-nanoparticle vaccines elicit potent neutralizing antibody responses. (cdc.gov)
  • The 'gold standard' method to assess humoral antibody responses fol owing vaccination is the neutralization assay. (who.int)
  • Natural infection by Zika virus but not DNA vaccination consistently elicits antibodies that compete with two potently neutralising monoclonal antibodies targeting distinct epitopes. (bvsalud.org)
  • We developed a blockade-of-binding (BOB) ELISA using A9E and G9E, two strongly neutralising ZIKV -specific monoclonal antibodies , which do not react with dengue virus . (bvsalud.org)
  • Human immunodeficiency virus (HIV)-1-specific broadly neutralizing monoclonal antibodies are currently under development to treat and prevent HIV-1 infection. (nature.com)
  • A chimeric virus, designated MN-III-2, was selected using anti-V3 loop monoclonal antibodies. (usda.gov)
  • A much more promising strategy is to use recombinant monoclonal antibodies (mAbs) against influenza and several are currently in clinical development ( 9 - 13 ). (frontiersin.org)
  • Discovery and characterization of prolactin neutralizing monoclonal antibodies for the treatment of female-prevalent pain disorders. (iasp-pain.org)
  • Here we report the discovery and characterization of first-in-class, humanized PRL neutralizing monoclonal antibodies (PRL mAbs). (iasp-pain.org)
  • We found that both the anti-SNV and anti-ANDV neutralizing antibody titers were significantly higher (p-value 0.0115) in the DSJI-vaccinated groups than the needle/syringe group. (nih.gov)
  • A construct encoding the RBD of the delta variant elicits robust neutralizing antibody response with neutralizing titers an order of magnitude above currently approved mRNA vaccines. (biorxiv.org)
  • The magnitude of neutralizing titers significantly increased with disease severity. (irsicaixa.es)
  • Longitudinal analysis confirmed the strong differences in neutralizing titers between non-hospitalized and hospitalized participants and showed rapid kinetics of appearance of neutralizing antibodies (50% and 80% of maximal activity reached after 11 and 17 days after symptoms onset, respectively) in hospitalized patients. (irsicaixa.es)
  • No significant impact of age, gender or treatment on the neutralizing titers was observed in this limited cohort. (irsicaixa.es)
  • The secondary endpoints were changes in anti-PGT121 antibody titers and CD4 + T-cell count, and development of HIV-1 sequence variations associated with PGT121 resistance. (nature.com)
  • MOSCOW - The Russian Direct Investment Fund (RDIF, Russia's sovereign wealth fund), investor in the Russian Sputnik V vaccine against coronavirus, today announced results of a study in Argentina on vaccine's IgG anti-spike titers and neutralizing capacity in non-infected and previously infected persons among healthcare workers. (healthofasia.com)
  • Adenovirus is often cultured from the pharynx and stool of asymptomatic children, and most adults have measurable titers of anti-adenovirus antibodies, implying prior infection. (medscape.com)
  • The animals were intramuscularly administered different vaccine doses, and the control and enzyme-linked immunosorbent assays (ELISA) and pseudovirus neutralization assays were used to determine the serum antibody titers. (news-medical.net)
  • During the live virus neutralization experiments, plaque reduction neutralization assays were used to measure the macaque serum samples, while cytopathic effect neutralization assays were used to determine the neutralizing antibody titers in hamster serum samples. (news-medical.net)
  • Designing immunogens that elicit broadly neutralizing antibodies to conserved viral epitopes on the major surface glycoprotein, spike, such as the receptor binding domain (RBD) is one potential approach. (biorxiv.org)
  • A modified vaccine antigen containing substitution mutations shared by the variants of concern could elicit broadly neutralizing antibodies against the circulating variants. (news-medical.net)
  • HIV-1 envelope gp41 broadly neutralizing antibodies: hurdles for vaccine development. (duke.edu)
  • Induction of binding Abs was measured by ELISA and neutralizing activity by PBMC and TZM-bl assays in all serum and vaginal samples. (europa.eu)
  • As demonstrated in Table 3, there was no significant correlation between days of symptom onset to collection of serum and antibody index/ratio to suggest waning of antibodies during the time period tested. (researchgate.net)
  • Passive transfer of serum antibodies from convalescent patients has been used in the past ( 7 , 8 ), however, this approach is of limited use in a global pandemic emergency. (frontiersin.org)
  • Based on our "know-how", a 3-in-1 dengue test (Dengue Day 1) has been developed by an Indian company for simultaneous point-of-care detection of Dengue NS1 Antigen & differential detection of IgM & IgG antibodies in Human Serum/ Plasma. (icgeb.org)
  • New combination vaccines should induce similar or superior levels of neutralizing antibody in serum for individual protection against paralytic disease and mucosal immunity that effectively decreases viral replication in the intestine and pharynx for population protection against transmission of poliovirus. (who.int)
  • We found that vaccination with the HPV16L1-58L2 cVLP formulated with alum plus monophosphoryl lipid A (Alum-MPL) adjuvant elicited robust neutralizing antibodies in both mice and rabbits against all tested HPV types including HPV16/31/33/35/52/58 (genus α9 ), HPV18/39/45/59/68 (genus α7 ), HPV6/11 (genus α10 ), HPV2/27/57 (genus α4 ), and HPV5 (genus β1 ). (oncotarget.com)
  • The multifunctional activity of the humoral immune response remained for at least 6 months after vaccination and was comparable to that of naturally acquired anti-MSP1 antibodies from semi-immune adults from Kenya. (nature.com)
  • The Omicron variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been shown to be highly transmissible and to evade neutralizing antibody immunity induced by vaccination and prior SARS-CoV-2 infection. (news-medical.net)
  • Here, we describe the safety, robust immunogenicity, and potent efficacy elicited in rhesus macaques by a modified vaccinia virus Ankara (MVA) vector expressing a full-length SARS-CoV-2 spike (S) protein (MVA-S). MVA-S vaccination was well tolerated and induced S and receptor-binding domain (RBD)-binding IgG antibodies and neutralizing antibodies against SARS-CoV-2 and several variants of concern. (frontiersin.org)
  • H3N2 influenza infection elicits more cross-reactive and less clonally expanded anti-hemagglutinin antibodies than influenza vaccination. (duke.edu)
  • The data also showed that pre-existing antibodies against the measles vaccine virus did not affect immunogenicity against chikungunya. (drugdiscoverynews.com)
  • Antibodies elicited by infection accumulate somatic mutations in germinal centers that can increase affinity for cognate antigens. (nih.gov)
  • Designed antigens to elicit neutralizing antibodies against sterically restricted antigen and method of using same. (utah.edu)
  • HIV envelope glycoprotein complexes elicit broadly cross-reactive neutralizing antibodies in rhesus macaques. (the-scientist.com)
  • In the early edition of Proceedings of the National Academy of Sciences , Timothy Fouts and colleagues at the University of Maryland Biotechnology Institute , Baltimore, US, show that crosslinked HIV-1 envelope-CD4 receptor complexes could elicit broadly cross-reactive neutralizing antibodies in rhesus macaques ( Proc Natl Acad Sci USA 2002, DOI/10.1073/pnas.182412199). (the-scientist.com)
  • They observed that in rhesus macaques, crosslinked complexes of soluble human CD4 and gp120 or gp140 envelope glycoproteins generated antibodies that neutralized a wide range of primary HIV-1 isolates. (the-scientist.com)
  • Does SARS-CoV-2 Omicron variant elicited neutralizing immunity enhance that elicited by the Delta variant? (news-medical.net)
  • In this paper, researchers studied whether the neutralizing immunity produced by Omicron also increases neutralizing immunity of the Delta variant. (news-medical.net)
  • The authors wanted to see if Omicron infection caused neutralizing immunity against the Delta variant. (news-medical.net)
  • Background It is to be determined whether people infected with SARS-CoV-2 will develop long-term immunity against SARS-CoV-2 and retain long-lasting protective antibodies after the infection is resolved. (researchgate.net)
  • It is currently unknown whether protection from DENV infection requires the presence of neutralizing antibodies, cell-mediated immunity, or both. (virology.ws)
  • mRNA-1345 is a vaccine against RSV encoding for a prefusion F glycoprotein, which elicits a superior neutralizing antibody response compared to the postfusion state. (pharmiweb.com)
  • The primary endpoint, defined as the presence of neutralizing antibodies against chikungunya, four weeks after administration of one or two MV-CHIK injections, was met across all treatment groups. (drugdiscoverynews.com)
  • We analyzed 6 independent groups of clonally related severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) Spike receptor-binding domain (RBD)-specific antibodies from 5 individuals shortly after infection and later in convalescence to determine the impact of maturation over months. (nih.gov)
  • Importantly, we found that purified anti-EDIII antibodies did not cross-react with closely related dengue virus (DENV) and therefore did not enhance DENV infection. (tghn.org)
  • Recovery from adenovirus infection is associated with the development of serotype-specific neutralizing antibodies. (medscape.com)
  • After SARS-CoV-2 infection, people are unlikely to produce long-lasting protective antibodies against this virus. (researchgate.net)
  • This information is in contrast to a study in Wuhan, China that found 10% of patients lost SARS-CoV-2 antibodies within weeks of infection (12) , although different serology tests were used in this case. (researchgate.net)
  • Our findings demonstrate AAV delivery of cross-subtype neutralizing nanobodies may be an effective strategy to prevent influenza infection and provide long-term protection independent of a host induced immune response. (frontiersin.org)
  • After a single injection, high neutralizing antibody levels were also generated in participants with evidence of prior SARS-CoV-2 infection, suggesting strong potential for development as a booster vaccine. (gsk.com)
  • Firstly, they can neutralize our infection-fighting first-responder antibodies, so they interfere with our body's ability to fight the infection if it persists. (medicalnewstoday.com)
  • Regardless of whether we produce first-line antibodies from the vaccine or a SARS-CoV-2 infection, anti-idiotype antibodies have the potential to produce the same symptoms and side effects as infection with the SARS-CoV-2 infection. (medicalnewstoday.com)
  • Dengvaxia presumably mimics an initial natural exposure in naïve recipients, so that a subsequent natural infection could potentially cause severe disease via antibody-dependent enhancement. (virology.ws)
  • For many viruses, antibodies are sufficient to protect against infection and/or disease. (virology.ws)
  • That is, by eliciting a strong response to all four DENV types, there wouldn't be the possibility of a weakly neutralizing antibody for one serotype causing a severe infection by that virus. (virology.ws)
  • Importantly, the cross-neutralizing antibody response was maintained at least 82 weeks in mice or 42 weeks in rabbits, and complete protection against HPV58 was observed at week 85 in mice. (oncotarget.com)
  • However, the ID device required at least two vaccinations in NHP to detect neutralizing antibodies in most animals, whereas all animals vaccinated once with the IM device seroconverted. (nih.gov)
  • The overall objective of EuroNeut-41 was to design a vaccine that raises antibodies able to prevent HIV cell fusion by blocking the virus fusion protein gp41 in its pre-fusogenic conformation. (europa.eu)
  • The protective effect of neutralizing antibodies in SARS-CoV-2 infected individuals is not yet well defined. (irsicaixa.es)
  • Any detectable titer of neutralizing antibody against poliovirus is considered protective against clinical paralytic diseases. (who.int)
  • We find that that a cocktail of homotrimeric sarbecovirus RBDs can elicit a neutralizing response to all components even in context of prior SARS-CoV-2 imprinting. (biorxiv.org)
  • Whether this HPS DNA vaccine, or any DNA vaccine, delivered by spring-powered DSJI will elicit a strong immune response in humans, requires clinical trials. (nih.gov)
  • Here, we show that the vaccine, termed SumayaVac-1 , elicited both a humoral and cellular immune response as well as a recall T cell memory. (nature.com)
  • Over this time, Omicron neutralization increased 14-fold, indicating a growing antibody response to the variation. (news-medical.net)
  • When comparing Omicron and Delta neutralization at the most recent time point, it was discovered that vaccinated participants were better able to mount a neutralizing response against the Delta virus. (news-medical.net)
  • However, it has been previously shown that more severe disease produces a stronger antibody response, and thus our mild disease cohort may result in a lower sensitivity (26). (researchgate.net)
  • Antibodies that the body produces after the initial immune response may also bind the same target cells as the SARS-CoV-2 virus. (medicalnewstoday.com)
  • Dengvaxia thus does not elicit a possibly critical element of the immune response. (virology.ws)
  • An effective DENV vaccine would generate a robust tetravalent response to all four serotypes at the same time, thus avoiding issues of antibody-dependent enhancement. (virology.ws)
  • A Prevalent Focused Human Antibody Response to the Influenza Virus Hemagglutinin Head Interface. (duke.edu)
  • The characteristics of this response would include production of neutralizing antibodies, generation of a T-cell response, and avoidance of immune-enhanced disease4. (who.int)
  • We evaluated the HPS DNA vaccine delivered using Stratis(®) and found that it produced high-titer anti-SNV and anti-ANDV neutralizing antibodies in rabbits (n=8/group) as measured by a classic plaque reduction neutralization test and a new pseudovirion neutralization assay. (nih.gov)
  • PGT121 is a monoclonal antibody isolated in 2011 from an African donor infected with HIV-1 subtype A, whose sera had demonstrated superior neutralization breadth and potency in an observational cohort, and so was considered an 'elite neutralizer' 9 . (nature.com)
  • In addition to increased affinity and neutralization potency, antibody evolution changed the mutational pathways for the acquisition of viral resistance and restricted neutralization escape options. (nih.gov)
  • For certain antibodies, affinity maturation enabled the neutralization of circulating SARS-CoV-2 variants of concern and heterologous sarbecoviruses. (nih.gov)
  • We have previously described R1a-B6, an alpaca-derived single domain antibody (nanobody), that is capable of potent cross-subtype neutralization in vitro of H1N1, H5N1, H2N2, and H9N2 influenza viruses, through binding to a highly conserved epitope in the influenza hemagglutinin stem region. (frontiersin.org)
  • Previously, we demonstrated that SNV and ANDV DNA vaccines encoding the virus envelope glycoproteins elicit high-titer neutralizing antibodies in laboratory animals, and (for ANDV) in nonhuman primates (NHPs). (nih.gov)
  • In conclusion, A9E and G9E BOB assays are sensitive and specific assays for detecting antibodies elicited by recent or remote ZIKV infections . (bvsalud.org)
  • Immunizations with diverse sarbecovirus receptor-binding domains elicit SARS-CoV-2 neutralizing antibodies against a conserved site of vulnerability. (garvan.org.au)
  • The neutralizing antibodies elicited by human papillomavirus (HPV) major capsid protein L1 virus-like particle (VLP)-based vaccines are largely type-specific. (oncotarget.com)
  • Once we use our antibodies to attack the invading viral protein, parts of these neutralizing antibody-antigen complexes can also be viewed as "not us" by our bodies. (medicalnewstoday.com)
  • Because YFV and DENV are both members of the family Flaviviridae, and because the DENV envelope protein is the major target of neutralizing antibody, this strategy would normally make sense. (virology.ws)
  • Mosaic-RBD-nanoparticles elicited antibodies with superior cross-reactive recognition of heterologous RBDs compared to sera from immunizations with homotypic SARS-CoV-2-RBD-nanoparticles or antibodies from COVID-19 convalescent human plasmas. (ox.ac.uk)
  • Recently, he created an innovative antibody platform, trispecific Abs, that show unprecedented anti-HIV breadth and potency. (wikipedia.org)
  • Mosaic RBD nanoparticles elicit neutralizing antibodies against SARS-CoV-2 and zoonotic coronaviruses. (ox.ac.uk)
  • These findings suggest that increasing antibody diversity through prolonged or repeated antigen exposure may improve protection against diversifying SARS-CoV-2 populations, and perhaps against other pandemic threat coronaviruses. (nih.gov)
  • Recent scientific evidence shows that antibodies created against the B.1.351 variant may provide broad cross-protection against other more transmissible variants. (gsk.com)
  • Passive transfer with the anti-EDIII neutralizing sera could confer protection against lethal ZIKV challenge in mice. (tghn.org)
  • Previously we have shown that these chimeric HRV14:HIV viruses can induce animals to make and can also bind antibodies that stop HIV from infecting cells in the laboratory. (usda.gov)
  • In these studies, bNAb resistance emerged in nearly all treated participants who received monotherapy, but in two notable cases 4 the repeated administration of a combination of two bNAbs in the setting of viremia maintained viral suppression for 3 months without development of resistance to either antibody. (nature.com)
  • Both IL-6 and IL-8 release are likewise diminished under these conditions compared with normal HBE, and treatment with TNF-a neutralizing antibody does not further inhibit cytokine release. (cdc.gov)