• isoelectric
  • Here, the same mechanism is accomplished in a commercially available electrophoresis chamber (see figure Equipment) for separating charged biomolecules, for example, superoxide dismutase (SOD) or allergens, at continuous pH conditions and different velocities of migration depending on different isoelectric points. (wikipedia.org)
  • tissues
  • In the case of solid tissues or cells, these are often first broken down mechanically using a blender (for larger sample volumes), using a homogenizer (smaller volumes), by sonicator or by using cycling of high pressure, and a combination of biochemical and mechanical techniques - including various types of filtration and centrifugation - may be used to separate different cell compartments and organelles prior to electrophoresis. (wikipedia.org)
  • page
  • Native-PAGE keeps the oligomeric form intact and will show a band on the gel that is representative of the level of activity. (wikipedia.org)
  • shift
  • Gel shift assays are often performed in vitro concurrently with DNase footprinting, primer extension, and promoter-probe experiments when studying transcription initiation, DNA replication, DNA repair or RNA processing and maturation, as well as pre-mRNA splicing. (wikipedia.org)