• Microbiology
  • As the field of microbiology becomes increasingly more centered on microbial community structure, exometabolomics has provided for rapid understanding of metabolic interactions between two or more species. (wikipedia.org)
  • plasmid
  • In a normal plasmid DNA preparation, multiple forms of DNA may be present, and gel from the electrophoresis of the plasmids would normally show a main band which would be the negatively supercoiled form, while other forms of DNA may appear as minor fainter bands. (wikipedia.org)
  • particles
  • It can also be used to separate large proteins, and it is the preferred matrix for the gel electrophoresis of particles with effective radii larger than 5-10 nm. (wikipedia.org)
  • Electrophoresis (from the Greek "Ηλεκτροφόρηση" meaning "to bear electrons") is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. (wikipedia.org)
  • This electrokinetic phenomenon was observed for the first time in 1807 by Russian professors Peter Ivanovich Strakhov and Ferdinand Frederic Reuss (Moscow State University), who noticed that the application of a constant electric field caused clay particles dispersed in water to migrate. (wikipedia.org)
  • Suspended particles have an electric surface charge, strongly affected by surface adsorbed species, on which an external electric field exerts an electrostatic Coulomb force. (wikipedia.org)
  • voltage
  • Each vector (angle of pulse) can be assigned its own voltage (field intensity) and its own switch time (duration of pulse). (bio-rad.com)
  • Extended runs across a low voltage gel yield the most accurate resolution. (wikipedia.org)
  • Once the nucleic acid is properly prepared, the samples of the nucleic acid solution are placed in the wells of the gel and a voltage is applied across the gel for a specified amount of time. (wikipedia.org)
  • procedure
  • However, when charges are not all uniform then, the electrical field generated by the electrophoresis procedure will affect the species that have different charges and therefore will attract the species according to their charges being the opposite. (wikipedia.org)
  • 2017
  • Denise P. Barlow (31 January 1950 - 21 October 2017) was a British geneticist who worked in the field of epigenomics. (wikipedia.org)
  • species
  • By placing the molecules in wells in the gel and applying an electric field, the molecules will move through the matrix at different rates, determined largely by their mass when the charge-to-mass ratio (Z) of all species is uniform. (wikipedia.org)
  • method
  • Thirty-three pulsotypes were identified by pulsed-field gel electrophoresis, and the majority belonged to clone C. In conclusion, the LAMP method developed for detecting A. baumannii was faster and simpler than conventional PCR and has great potential for both point-of-care testing and basic research. (nih.gov)
  • composition
  • The limit of resolution depends on gel composition and field strength. (wikipedia.org)
  • In most cases, the gel is a crosslinked polymer whose composition and porosity is chosen based on the specific weight and composition of the target to be analyzed. (wikipedia.org)
  • angle
  • one that runs through the central axis of the gel and two that run at an angle of 60 degrees either side. (wikipedia.org)
  • wells
  • If several samples have been loaded into adjacent wells in the gel, they will run parallel in individual lanes. (wikipedia.org)
  • electric
  • The electric field consists of a negative charge at one end which pushes the molecules through the gel, and a positive charge at the other end that pulls the molecules through the gel. (wikipedia.org)
  • The electric field also exerts a force on the ions in the diffuse layer which has direction opposite to that acting on the surface charge. (wikipedia.org)
  • Cell
  • Although the magnitude of the inter-experiment variations limit the precision with which cell survival and DNA electrophoresis can be compared, the data do support a simple correlation between these two measures of response. (lancs.ac.uk)
  • limit
  • The detection limit was 50 pg/μl, which was about 10-fold greater than that of PCR.Thirty-three pulsotypes were identified by pulsed-field gel electrophoresis, and the majority belonged to clone C.We further demonstrated a high distribution of class D carbapenemase-encoding genes, mainly OXA-23, which presents an emerging threat in hospitals in China. (nih.gov)
  • large
  • For extremely large molecules (up to around 2 Mb), switching-interval ramps can be used that increases the pulse time for each direction over the course of a number of hours-take, for instance, increasing the pulse linearly from 10 seconds at 0 hours to 60 seconds at 18 hours. (wikipedia.org)
  • However
  • However, with periodic changing of field direction, the various lengths of DNA react to the change at differing rates. (wikipedia.org)
  • Low-concentration gels (0.1 - 0.2%) however are fragile and therefore hard to handle. (wikipedia.org)
  • However, Debye length must be important for electrophoresis, as follows immediately from the Figure on the right. (wikipedia.org)
  • different
  • The different sized molecules form distinct bands on the gel. (wikipedia.org)
  • citation needed] Bands in different lanes that end up at the same distance from the top contain molecules that passed through the gel with the same speed, which usually means they are approximately the same size. (wikipedia.org)
  • PAGE
  • Similarly, RNA and single stranded DNA can be run and visualised by PAGE gels containing denaturing agents such as Urea. (wikipedia.org)
  • charge
  • That is, larger pieces of DNA will be slower to realign their charge when field direction is changed, while smaller pieces will be quicker. (wikipedia.org)
  • known
  • Exometabolomics, also known as 'metabolic footprinting', is the study of extracellular metabolites and is a sub-field of metabolomics. (wikipedia.org)