There are 4 steps to this method: polymerase chain reaction (PCR) amplification of DNA sequence of interest denaturation of double-stranded PCR products cooling of the denatured DNA (single-stranded) to maximize self-annealing detection of mobility difference of the single-stranded DNAs by electrophoresis under non-denaturing conditions. (wikipedia.org)
The aim of this study was to detect carriers of CVM in Holstein population using Polymerase Chain Reaction- Primer-introduced restriction analysis and Restriction Fragment Length Polymorphism (PCR-PIRA and RFLP) methods. (researchgate.net)
SSCP3
These had all been previously screened for the presence of p53 mutations in exons 2 and 4-11 by single strand conformational polymorphism (SSCP) analysis and DNA sequencing as previously described. (bmj.com)
SequaGel MD is a proprietary formulation, supplied as a 2X stock, designed to resolve sequence-related differences by SSCP (single-strand conformational polymorphism) and heteroduplex analysis. (nationaldiagnostics.com)
Two principal methods of conformational analysis have been developed, SSCP Analysis and heteroduplex analysis. (nationaldiagnostics.com)
Gradient gel electrophoresis1
In this study, the authors performed mutation analysis of these three genes in 41 sporadic HSCR patients without any family history by using single-strand conformational polymorphism or denaturing gradient gel electrophoresis methods. (drugbank.com)
Conformation4
Single strand conformation polymorphism analysis of candidate genes for reliable identification of alleles by capillary array electrophoresis. (usda.gov)
The technique uses the method based on the fact that single-stranded DNA has a defined conformation. (wikipedia.org)
Any altered conformation due to a single base change in the sequence can cause single-stranded DNA to migrate differently under nondenaturing electrophoresis conditions, so a wild-type and mutant DNA samples display different band patterns. (wikipedia.org)
Aggregation to form fibrils is also highly heterogeneous in terms of the number of strands in a fibril and the elongation speed and conformation of fibrils. (biorxiv.org)
Genetic4
This technique is when genetic microscopes are used to look down to a single cell it is used to look at recombination also look for mutations in genes it has been used to help identify many deleterious alleles in genes. (wikipedia.org)
Since a variation of a single nucleotide in a sequence may indicate a significant genetic anomaly, an extremely sensitive method to analyze these mutations is necessary. (nationaldiagnostics.com)
Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. (lookformedical.com)
Genetic variability analyses have proven essential to facilitate an improved understanding of the evolution of the papillomavirus. (biomedcentral.com)
Capillary Electrophoresis1
This method is based on the extension of a primer that anneals immediately adjacent to the single-nucleotide polymorphism with fluorescent dideoxynucleotide triphosphates (minisequencing), followed by analysis on an ABI Prism 3700 capillary electrophoresis instrument. (ox.ac.uk)
Double-stranded1
The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. (lookformedical.com)
Nucleotide6
DNA analysis is used to help determine the species of an animal they use DNA nucleotide sequencing as a key method and follow it up by comparing sequenced DNA fragments with reference DNA sequences of different species. (wikipedia.org)
Surprisingly, the sequence analysis of PCR product also revealed the presence of two previously unknown homozygous mutations (TG→CT) at nucleotide positions 554 and 555 in addition to the previously reported heterozygous mutation at position 559. (researchgate.net)
By optimised primer design it was possible to type the 4 relevant single nucleotide polymorphisms (SNPs) in the prnp simultaneously in one multiplex reaction. (biomedcentral.com)
Nanoscaffolding and nanoquenching properties of thiol-capped gold nanocrystals (GNCs), covalently linked to fluorophore-lebeled oligonucleotide through metal-sulfur bond, were extensively studied for decades to detect specific sequences and single-nucleotide mismatches [ 1 - 3 , 21 , 22 ]. (hindawi.com)
It was found that single nucleotide mutation in the VRN-box can influence the vernalization requirement and growth habit of wheat. (biomedcentral.com)
Furthermore, the analysis of HPV6 E6 and E7 sequences revealed seven/five single-nucleotide mutations, two/four and five/one of which were non-synonymous and synonymous, respectively. (biomedcentral.com)
Gene7
Our results show that one out of 60 bulls tested exhibited polymorphism (G→T) at position 559 in exon 4 of SLC35A3 gene. (researchgate.net)
Therefore, the demand on genotyping capacities of the polymorphisms of the prion protein gene ( prnp ) relevant to presently known disease associations and EU regulations is steadily increasing. (biomedcentral.com)
Susceptibility to scrapie is largely controlled by three polymorphic amino acid positions (136, 154, 171) of the ovine prion protein gene ( prnp ) [ 4 ] and reliable genotyping of the corresponding DNA polymorphism is required as a basis for selection decisions. (biomedcentral.com)
Both p53 wild type and p53 mutant samples were included in the p16 INK4a analysis to determine whether germline alterations of one gene precluded the occurrence of alterations of the other. (bmj.com)
Analysis of 18 novel mutations in the factor VIII gene. (eahad.org)
Ectopic mRNA analysis and molecular modelling substantiate severe haemophilia in a patient with a FVIII gene splice mutation. (eahad.org)
In this paper, we have targeted a 27-bp AluI fragment of porcine mitochondrial cytochrome b (cytb) gene to construct porcine-specific hybrid nanobiosensor probe that was able to detect pork in heterogeneous autoclaved meat products in a single step eliminating the need of washing or separation steps. (hindawi.com)
Exon1
Direct sequencing covering the region of exon 2 encoding amino acid positions 136, 154 and 171 is the most accurate method, that enables the typing of additional and detection of hitherto unknown polymorphisms. (biomedcentral.com)
Detection2
Hemophilia A. Detection of molecular defects and of carriers by DNA analysis. (eahad.org)
DNA microarray analysis for the detection of mutations in hemophilia A. (eahad.org)
Genes2
Mutation analysis of the RET, the endothelin-B receptor, and the endothelin-3 genes in sporadic cases of Hirschsprung's disease. (drugbank.com)
The selection pressures acting on the E6 / E7 genes were estimated by Phylogenetic Analyses by Maximum Likelihood version 4.8 software. (biomedcentral.com)
Genomic1
Agarose gel electrophoresis of linear genomic DNA in the presence of ethidium bromide: band shifting and implications for forensic identity testing. (mcmaster.ca)
Prion1
This work investigates the molecular basis of the phenotypic heterogeneity of prion diseases through a multi-omics analysis of the two most common sCJD subtypes: MM1 and VV2. (biomedcentral.com)
Fluorescent1
In this study we combined the amplification refractory mutation system (ARMS) with standard fluorescent based fragment length analyses method to develop a prnp genotyping method (PRNP ARMS). (biomedcentral.com)
Methods6
Mitochondrial microsatellite analysis methods are often performed to individualize the remains of an animal and determine if a species is endangered, or if it was hunted out of season. (wikipedia.org)
Length Polymorphism (PCR-PIRA and RF LP) methods. (researchgate.net)
It provides an efficient and inexpensive diagnostic mutation analysis that will improve the quality of prnp genotyping compared with other low-cost methods. (biomedcentral.com)
Moreover, the recent report on frequent artifacts on real-time PCR results [ 33 ] has raised the questions of validity of the existing DNA-based methods in the analysis of highly processed meat products. (hindawi.com)
We developed and used new single-molecule fluorescence spectroscopic and fluorescence lifetime imaging methods, combined with deep learning for image analysis. (biorxiv.org)
Phylogenetic trees were constructed by Maximum-likelihood and the Kimura 2-parameters methods by Molecular Evolutionary Genetics Analysis version 6.0. (biomedcentral.com)
Sequence3
The results of these analyses revealed that both the HPV6 E6 and E7 were highly conserved within the analyzed patient samples, and comprised only 3 types of variant sequence, respectively. (biomedcentral.com)
Analysis of the P. asymbiotica genome identified a novel fucose-binding lectin designated PHL with a strong sequence similarity to the recently described P. luminescens lectin PLL. (bvsalud.org)
Based on the sequence analysis, PHL could contain up to twelve binding sites per monomer. (bvsalud.org)
Mutation sites2
The phylogenetic analyses of the E6 and E7 sequences indicated that they belonged to sub-lineage A1 and sub-lineage B1, whereas the selective pressure analyses showed that only the E7 mutation sites 4R, 34E, and 52F were positive selection. (biomedcentral.com)
Because the next codons 53 and 55 are known as major mutation sites for drug resistance, more detailed analysis using more samples is needed to determine whether it influences susceptibility to dapsone and/or efficiency of folate biosynthesis in M. lepromatosis or not. (bvsalud.org)
Heterogeneous2
Polymorphism in the structure of fibrils formed by aggregation suggests the existence of many different assembly pathways and therefore a heterogeneous ensemble of soluble oligomers. (biorxiv.org)
This polymorphism indicates that the entire aggregation process, including oligomerization, should be heterogeneous, which complicates biophysical and biochemical characterizations of Aβ. (biorxiv.org)
Heterogeneity1
Single-molecule spectroscopy can be an effective tool to probe heterogeneity because molecular species can be observed one at a time, so individual oligomers can be detected without separation. (biorxiv.org)
Human2
Unlike human identification, animal identification requires determination of its family, genus, and species, and sex in order to individualize the animal, typically through the use of DNA based analyses. (wikipedia.org)
Thus, while inculcating the UBPs in a DNA structure, their aromatic scaffolds must exhibit hydrophobic packing and must have hydrogen bond donor groups for the artificial nucleotides to interact with polymerases for their efficient incorporation in the non-template strand to bind with their UB partner in template strand [1-3]. (fortuneonline.org)
Diagnostic1
Perform further specific diagnostic testing, including DNAmutation analysis, to confirm the underlying diagnosis (see Other Tests). (medscape.com)
Samples1
PCR product samples were then visualised using agar gel electrophoresis and viewed using the unified gel documentation system. (ugm.ac.id)
Confirm1
To confirm this polymorphism, the PCR product was purified using ExoSAP-IT followed by sequencing by Applied Biosystems 3130XL Automated Sequencer using the ABI BigDye Ver 3.1. (researchgate.net)
System1
A 2-tiered system of analysis has been proposed that analyzes newborn CPK from dried blood spots followed up with DNA analysis from that same dried blood spot if the CPK is elevated. (medscape.com)
Determine1
Isotope analysis is used in this same vein to determine the composition of the habitat that animal resides in. (wikipedia.org)
Steps1
In this initial study, we combine single-molecule Förster resonance energy transfer (FRET) spectroscopy, fluorescence lifetime imaging (FLIM), and image analysis using deep learning (codes are available at https://github.com/hoisunglab/FNet ) to interrogate several steps during the aggregation process of the 42-residue Aβ peptide (Aβ42), including dimerization, formation of stable oligomers, and fibril elongation. (biorxiv.org)