Theoretically, this approach decreases the risk of insertional mutagenesis compared with repeated, separate integrations of multiple genes and associated promoters into the T-cell genome. (ubigene.us)
Transgenesis and insertional mutagenesis2
These integration systems are ideally suited to transgenesis and insertional mutagenesis strategies in the frog. (biomedcentral.com)
Since the first discovery of DNA transposons in Maize by Barbara#McClintock in 1950 [ 1 ], transposons have been used extensively as genetic tools in invertebrates and in plants for transgenesis and insertional mutagenesis [ 2 - 7 ]. (biomedcentral.com)
Vectors9
Transposon vectors can be used in the frog for transgenesis and for insertional mutagenesis where enhancer trap and gene trap constructs are used to identify genomic loci involved in developmental processes. (biomedcentral.com)
Viral vectors also have the largest genomic footprint of iPSC generating methods due to the risk of insertional mutagenesis. (addgene.org)
Gene delivery strategies based on viral vectors are currently the most efficient. (tamu.edu)
Despite the broad tropism of VSV-G, lentivirus vector transduction is less efficient than adenoviral vectors. (alecomm.com)
Third generation lentiviral vectors currently in use encode no viral proteins, only a few cis-acting sequence elements required for packaging, reverse transcription, and efficient expression. (alecomm.com)
It has been speculated that accidental exposure to lentiviral vectors could result in insertional mutagenesis, a fear found on observed tendency for the closely related γ-retroviral vectors to integrate in proximity to proto-oncogenes. (alecomm.com)
Extensive analysis has shown though that lentiviral vectors have a very different preference for insertion sites, and so hazardous insertional mutagenesis is unlikely. (alecomm.com)
To achieve efficient genetic material delivery in mammalian cells, viral-derived vectors have been served as the most promising tools. (creative-biolabs.com)
In this important study, Drs. Thomas and Capecchi used both replacement and insertional vectors to introduce a neomycin resistance (neo r ) gene into exon 8 of the Hprt gene, showing that clones of transfected cells had lost HPRT function (becoming 6-TG resistant), and had gained Neo r activity. (ijbs.com)
Genome4
Until recently, however, functional analysis of the DED fungi was hampered by the lack of protocols for efficient gene knockout and by the unavailability of a full genome sequence. (sisef.it)
The high fecundity of the frog combined with the ability to remobilize transposon transgenes integrated into frog genome will allow large-scale insertional mutagenesis screens to be performed in laboratories with modest husbandry capacities. (biomedcentral.com)
Dr. Burgess is now merging large-scale mutagenesis and phenotype characterization into a novel approach to functionally annotate a vertebrate genome. (nih.gov)
Because of their ability to generate insertions, IS elements and transposons represent a useful and efficient tool in biotechnology by introducing 'foreign' DNA into the genome of various plants, animals or bacteria (for a review, see Coates et al. (proteasomesignaling.com)
Site-directed mu1
In order to generate nonflagellated mutants of Salmonella Enteritidis, a new site-directed mutagenesis system has been developed and applied. (proteasomesignaling.com)
Transposon2
Insertional transposon mutagenesis is a frequently used technique with the enormous advantage not only of the generation of new phenotypes, but the identification of the mutated gene directly. (proteasomesignaling.com)
Finally, retroviruses are prone to insertional mutagenesis and random integration creates variation between iPSC clones. (addgene.org)
tax causes malignant transformation not through integration and insertional mutagenesis, as many retroviruses do, but by inhibition of DNA repair (base excision pathway) and trans-activating disruption of cellular growth control mechanisms ( 2 ). (cdc.gov)
Genes6
While the selective inactivation of Ophiostoma genes by insertional mutagenesis remains a challenge, an alternative approach based on RNA interference is now available for down-regulating the expression of targeted genes. (sisef.it)
Efficient integration of functional genes is an essential prerequisite for successful gene delivery such as cell transfection, animal transgenesis, and gene therapy. (tamu.edu)
As a complementary resource, chemically induced mutants have been shown to provide an efficient alternative because each individual line can bear single point missense and nonsense substitutions in hundreds of genes [ 7 ]. (biomedcentral.com)
More recently, Dr. Burgess has turned his mutagenesis efforts to targeted knockouts using CRISPR/ Cas9 technology, gaining full control over which genes are inactivated. (nih.gov)
MMTV causes tumors by insertional mutagenesis, i.e., viral DNA integration near Wnt or Fgf genes, for breast cancer induction. (utexas.edu)
Multiple mutations affecting embryonic development have been induced by chemical and insertional mutagenesis resulting in the identification of genes with important roles in development in Drosophila [ 23 - 25 ]. (biomedcentral.com)
Gene therapy3
In recent years, these powerful genetic tools have been used in higher vertebrates for transgenesis, insertional mutagenesis, and gene therapy applications. (biomedcentral.com)
The results presented herein demonstrate that our helper-independent plasmids represent an important step in the development of safe and efficient gene delivery methods that should prove valuable in gene therapy and transgenic approaches. (tamu.edu)
DNA transposons have emerged as indispensible tools for manipulating vertebrate genomes with applications ranging from insertional mutagenesis and transgenesis to gene therapy. (biomedcentral.com)
Mammalian1
This work led to his important conclusion that mammalian somatic cells possess an efficient enzymatic machinery for mediating homologous recombination [ 6 ]. (ijbs.com)
Screens2
The rapid accumulation of genetic resources for this animal and the success of pilot mutagenesis screens have helped propel this model system forward. (biomedcentral.com)
Knowledge of the genetic networks controlling embryogenesis has been obtained principally by mutagenesis screens in model species. (biomedcentral.com)
Transformation1
Efficient transformation facilitated insertional mutagenesis as described further below. (wikipedia.org)
Integration1
Site-directed integration/mutagenesis systems are used to carry out targeted transpositions on DNA. (proteasomesignaling.com)
Insertion1
Site-directed or targeted mutagenesis mediated by insertion sequence (IS) elements and transposons relates to the use of a novel recombinant DNA technology for the targeted modification of DNA. (proteasomesignaling.com)
Cas91
In this report, we described the application of CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (endonuclease) system for efficient genetic editing in E. tenella , and showed that the CRISPR/Cas9 system mediates site-specific double-strand DNA breaks with a single guide RNA. (biomedcentral.com)
Genetic2
Due to lack of efficient in vitro tissue culture model and an effective selection marker, the technical capability for genetic modification of Eimeria has lagged behind for that of Toxoplasma and Plasmodium . (biomedcentral.com)
Dr. Burgess' mutagenesis and phenotyping efforts address a variety of questions ranging from systematically characterizing cell signaling molecules to generating animal models for rare genetic diseases. (nih.gov)
Sequences1
Over several serial transfers, particular sequences accumulated, and in isolation these were shown to out-compete less efficient replicators. (korea.ac.kr)
Expression2
The enhancers, along with the LTR promoter sequence, supported efficient expression of the corrective IL2RG gene and allowed successful gene correction. (europeanhealthjournal.com)
Transcriptomic analyses provide an efficient approach to explore the temporal gene expression profiles in embryos and to search for the developmental regulators. (biomedcentral.com)
Sequence1
He reasoned that if this machinery could be efficient for homologous recombination between a newly introduced DNA molecule and the endogenous DNA sequence, any host gene could be mutated. (ijbs.com)
Cell2
At the subcellular level, CD19/20/22CAR T-cells formed dense immune synapses with target cells that mediated effective cytolytic complex formation, were efficient serial killers in single-cell tracking studies, and were as efficacious as CD19CAR T-cells against primary CD19(+) disease. (ubigene.us)
In their paper, they concluded 'It is hoped that this combination of using ES cells as the recipient cell line and site-specific mutagenesis achieved by gene targeting will provide the means for generating mice of any desired genotype" [ 2 ]. (ijbs.com)