• The same extracts were assessed for their antioxidative potentials with the use of DPPH free radical scavenging assay followed by determination of HRBC membrane stabilization method, Brine Shrimp Lethality Assay (BSLA) and GC-MS analysis. (biomedcentral.com)
  • During Brine Shrimp Lethality Assay, all extracts of BG were found to be bioactive and the degree of lethality was found to be concentration dependent. (biomedcentral.com)
  • The Brine Shrimp Lethality Assay found extracts to be bioactive suggesting extracts as a promising candidate for plant-derived anti-tumor compounds. (biomedcentral.com)
  • The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
  • The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
  • Moreover, the algorithm was able to distinguish cytotoxic vs. cytostatic drug effects and drug-resistant clones that will give rise to later relapse. (technologynetworks.com)
  • Additionally, the algorithm was able to distinguish between cytotoxic and cytostatic effects of drugs and drug-resistant clones that will cause subsequent relapse. (genericviabuy.com)
  • Studies have found very good relationship between this simple, inexpensive, and bench-top assay and the antitumor potential of the cytotoxic compounds [ 10 ]. (biomedcentral.com)
  • Within natural diffusion limit of vascularized tissue, tumor-derived MOS allowed sufficient penetration by therapeutic immune T-cells such as CAR-T, enabling a novel T cell potency assay to assess tumor killing by the engineered T-cells. (technologynetworks.com)
  • MOS could be further integrated with deep-learning imaging analysis for rapid drug testing of small and heterogeneous clinical tumor biopsies. (technologynetworks.com)
  • Secarna Pharmaceuticals, an IZB-based biopharmaceutical company focusing on the discovery and development of next-generation antisense oligonucleotide (ASO) therapies to address challenging or previously undruggable targets via its LNAplus™ platform, announced the publication of striking new preclinical data demonstrating that bimodal ASOs can enable efficacious long-term antitumor immunity in tumor models compared to current immune checkpoint inhibitors. (izb-online.de)
  • The combined downregulation of PD-L1 expression in a single-molecule approach resulted in exceptional antitumor activity in different tumor models. (izb-online.de)
  • Collectively, these data show that IM-T9P1 leads to long-lasting, adaptive antitumor immunity that can be further improved when combined with T cell-targeting immune checkpoint inhibitors and can have efficacy against large, established tumors in preclinical tumor models. (izb-online.de)
  • Treatment with IM-T9P1 resulted in the sustained activation of a specific type of tumor infiltrating cell, dendritic cells 3 (DC3s), which can promote antitumor immunity by supporting T cells with survival signals and enabling them to perform their effector functions. (izb-online.de)
  • In the present work, the effects of PnV on tumor development were established in vivo using a xenogeneic model. (nature.com)
  • Therefore, it would be relevant to establish the effects of PnV on tumor development in vivo , considering the complex neoplastic microenvironment. (nature.com)
  • Anticancer effects of synthetic phosphoethanolamine on Ehrlich ascites tumor: an experimental study. (jscimedcentral.com)
  • Moreover, common targeted therapeutic drug formulations have weak stability in malignant tumor leukemia, and their application effects are limited. (frontiersin.org)
  • Therefore, based on the characteristics of black phosphorus two-dimensional nanomedicine, experimental designs were conducted on its nanosheet preparation, polyethylene glycol modification, and anti-tumor drug loading. (frontiersin.org)
  • Its drug release characteristics perform well in photothermal therapy, indicating that black phosphorus nano drugs can improve the biological safety and applicability of drugs in new tumor targeted therapy. (frontiersin.org)
  • Targeted therapy uses targeted drugs to maintain a high concentration of the drug in the tumor area, improving the killing efficiency of cancer cells while avoiding damage to other normal cells. (frontiersin.org)
  • examined the mechanism of action underlying the anti-tumor effects of the complementarity-determining region (CDR) C36L1 synthetic peptide derived from the V L CDR1 of the C36 Fab fragment of the anti-vaccinia immunoglobulin. (cytoskeleton.com)
  • Here, the authors investigated whether its microtubule-destabilizing effect is responsible for its anti-tumor activity. (cytoskeleton.com)
  • The authors found that the peptide induces apoptotic effects on multiple cancer cells both in vitro and in vivo , inhibits tumor cell migration and invasion, and arrests cell cycle primarily in the G2M phase. (cytoskeleton.com)
  • BK011P) was an essential reagent in this study, complementing the in vivo results and in vitro cell culture data which demonstrated conclusively that the C36L1 peptide targets microtubules in exerting its anti-tumor effects. (cytoskeleton.com)
  • Increasing amounts of evidence indicate that autophagy is induced by a number of stressors in tumor cells, such as starvation, growth factor deprivation, hypoxia, damage stimulation and therapeutic drugs, and is an important survival mechanism in response to cellular stress ( 9 ). (spandidos-publications.com)
  • Discuss the current challenges and key considerations to be taken when designing assays to identify new biomarkers in pan tumor applications. (hub-xchange.com)
  • Therefore, a rationale for treating hypertension would be to administer drugs or natural compounds which selectively inhibit ACE. (scielo.br)
  • The former issue was often neglected prior to initiating drug discovery programmes while the latter was considered impossible because it was purported that antibodies were unsuitable to derive compounds. (cancer.gov)
  • This seemed much larger than would be expected for the range of space occupied by drug-like chemical compounds, estimated at 300 to 1000 Å2 [4, 5]. (cancer.gov)
  • We exploited the specific binding of the antibody fragment and were able to identify RAS-binding compounds in antibody-competition assays (illustrated in Fig.1). (cancer.gov)
  • 50 years of preclinical anticancer drug screening:empirical to target-driven approaches. (jscimedcentral.com)
  • The objective of this review was to analyze the results from preclinical and clinical trials of these drugs for the treatment of cancer. (frontiersin.org)
  • Aslthough all these drugs have produced good results in preclinical studies of multiple cancers, the outcomes of clinical trials have not been similar. (frontiersin.org)
  • This assay has been extensively used for different studies such as for preliminary toxicity screening of plant extracts, detection of fungal toxins, plant extract toxicity, heavy metals, cyanobacteria toxins, pesticides, and cytotoxicity testing of dental materials [ 9 ]. (biomedcentral.com)
  • Metronidazole (Flagyl) is a drug used to treat infections and has been studied in the treatment of cancer [10,11] as a radiosensitizer drug, especially for hypoxic cells [9]. (who.int)
  • tizer drug, especially for hypoxic cells [ 9 ]. (who.int)
  • Corresponding cultures yielded a strain identified as M . haemophilum at the Netherlands National Institute for Public Health and the Environment (RIVM) by using the Inno-LiPA Mycobacteria v2 reverse line blot assay (Innogenetics, Ghent, Belgium). (cdc.gov)
  • Using cDNA microarray screens and northern blot analysis, a previous study showed that Beclin 1 is upregulated in liver cancer tissues ( 11 ). (spandidos-publications.com)
  • The effect of venom was also evaluated on macrophages in vitro . (nature.com)
  • A recent in vitro screening of the Phoneutria nigriventer spider venom (PnV) antitumor effects by our group has shown that the venom significantly affected glioblastoma cell lines. (nature.com)
  • Untargeted Metabolomics Study of the In Vitro Anti-Hepatoma Effect of Saikosaponin d in Combination with NRP-1 Knockdown. (cancer-genetics.org)
  • Sphingolipid-targeting drugs have been tested alone or in combination with chemotherapy, exhibiting antitumor activity alone and in synergism with chemotherapy in vitro and in vivo . (frontiersin.org)
  • The in vitro effects of metronidazole on the production of reactive oxygen species by polymorphonuclear (PMN) cells were studied by means of nitroblue tetrazolium and luminol-dependent chemiluminescence. (who.int)
  • To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)
  • One such biological target was RAS, where decades of attempts had failed (until very recently) to yield anti-cancer drugs directly interfering with RAS function. (cancer.gov)
  • During his six year stay at GNF, Dr. Gray became the director of biological chemistry where he supervised a group of over fifty researchers integrating chemical, biological and pharmacological approaches towards the development of new experimental drugs. (stanford.edu)
  • There, he has established a discovery chemistry group that focuses on developing first-in-class inhibitors for newly emerging biological targets, including resistant alleles of existing targets, as well as inhibitors of well-validated targets, such as Her3 and RAS, that have previously been considered recalcitrant to small molecule drug development. (stanford.edu)
  • alpha- and beta-interferon crossreact with common receptors, while gamma-interferon initiates its biological effects through its own specific receptor system. (lookformedical.com)
  • Introduction The research of Hatch (1) has greatly increased our understand- ing of the use and potential limitations of biological markers for adverse reproductive effects. (cdc.gov)
  • Fenugreek has been documented to have uterine stimulant effects and has been used in traditional medicine to induce childbirth. (drugs.com)
  • Its low toxicity, high efficiency, and controllable drug release advantages make it have good application effects. (frontiersin.org)
  • In contrast, minor adverse effects restricted to a few subjects and hepatic toxicity have been observed in clinical trials of ABC294640 and safingol, respectively. (frontiersin.org)
  • Many of the medical complications that arise are the direct consequence of the need to administer potent immunosuppressive agents, with their attendant risks of infection, malignancy and drug toxicity. (ersjournals.com)
  • However, these are associated with an increased risk of adverse gastrointestinal, renal and cardiovascular effects [ 6 ]. (biomedcentral.com)
  • Non-steroidal anti-inflammatory drugs (NSAIDs) have a variety of potential indications that include management of pain and inflammation as well as chemoprevention and/or treatment of cancer. (nih.gov)
  • Immunosuppressive agents encompassed a large number of drugs, mainly represented by anti-tumour necrosis factor (TNF)-α, anti-retroviral therapy (ARV), azathioprine (AZT), methotrexate (MTX), mycophenolate mofetil (MMF), non-steroidal anti-inflammatory drugs (NSAIDs), rituximab and steroids. (ersjournals.com)
  • NSAIDS are widely used for their anti-inflammatory, analgesic and antipyretic activity and are among the most widely used drugs worldwide [ 6 ]. (biomedcentral.com)
  • Patients can usually be treated with nonsteroidal anti-inflammatory drugs (NSAIDs) alone. (msdmanuals.com)
  • As documented in a recent publication in Stem Cell Reports , their MOS can be used as patient avatars for studies involving direct viral infection, immune cell penetration and high-throughput therapeutic drug screening, something that is not obtainable with conventional patient-derived models. (technologynetworks.com)
  • Wolber G, Seidel T, Bendix F, Langer T. Molecule-pharmacophore superpositioning and pattern matching in computational drug design. (jscimedcentral.com)
  • The clandestine chemist has to switch their method of production according to the availability of different starting materials, thus a number of synthetic routes are available to a given drug molecule. (soci.org)
  • Therefore, Src has been considered a target molecule for drug development. (oncotarget.com)
  • Nathanael Gray is the Krishnan-Shah Family Professor of Chemical and Systems Biology at Stanford, Co-Director of Cancer Drug Discovery Co-Leader of the Cancer Therapeutics Research Program, Member of Chem-H, and Program Leader for Small Molecule Drug Discovery for the Innovative Medicines Accelerator (IMA). (stanford.edu)
  • Our data also reveal that the inhibitory effect of rhodomycin A on lung cancer progression may act through suppressing the Src-related multiple signalling pathways, including PI3K, JNK, Paxillin, and p130cas. (oncotarget.com)
  • The inhibitory effect was in a dose-dependent pattern. (who.int)
  • Potential molecular mechanism supporting this synergistic effect was assessed with emphasis on determining whether the combination treatment enhanced inhibition of the anti-apoptotic signal transducers Akt and NF-kB leading to increased activation of caspase mediated apoptosis. (rgcb.res.in)
  • In addition, rhodomycin A rendered gefitinib-resistant lung adenocarcinoma cells more sensitive to gefitinib treatment, implying a synergistic effect of the combination therapy. (oncotarget.com)
  • Prior to Flagship Biosciences, he served in senior scientific roles in oncology drug development for OSI Pharmaceuticals and Pfizer. (hub-xchange.com)
  • These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)
  • This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
  • Sawa M, Masai H. Drug design with Cdc7 kinase: a potential novel cancer therapy target. (jscimedcentral.com)
  • Dr. Gray's research has had broad impact in the areas of kinase inhibitor design and in circumventing drug resistance. (stanford.edu)
  • Dr. Gray has also developed structure-based, generalized approaches for designing drugs to overcome one of the most common mechanisms of resistance observed against most kinase inhibitor drugs, mutation of the so-called 'gatekeeper' residue, which has been observed in resistance to drugs targeting BCR-ABL, c-KIT and PDGFR. (stanford.edu)
  • These drugs inhibit immune-suppressive signals and reinstate cancer immunosurveillance. (izb-online.de)
  • Mechanism-based target identification and drug discovery in cancer research. (jscimedcentral.com)
  • Li X, Taratula O, Taratula O, Schumann C, Minko T. LHRH-Targeted Drug Delivery Systems for Cancer Therapy. (jscimedcentral.com)
  • In this study, the anticancer effect of DEH on human colorectal cancer and its underlying mechanism were evaluated. (biomedcentral.com)
  • However, the anticancer effects of DEH on colorectal cancer and the mechanism of action are still unclear. (biomedcentral.com)
  • Terry Rabbitts is Professor of Molecular Immunology in the Centre for Cancer Drug Discovery, Institute of Cancer Research, Sutton, London, UK. (cancer.gov)
  • The effectiveness or lack of a major therapeutic effect of sphingolipid modulation by some drugs as a cancer therapy and other aspects related to their mechanism of action are discussed in this review. (frontiersin.org)
  • In 2021, Dr. Gray joined Stanford University where he has joined the Stanford Cancer Institute, Chem-H and the Innovative Medicines Accelerator (IMA) to spur the development of prototype drugs. (stanford.edu)
  • The identification, optimization and synthesis of antineoplastic drugs from PnV molecules may lead to a new multitarget chemotherapy. (nature.com)
  • MOS can be further integrated with deep learning image analysis for rapid drug testing of clinical biopsies of small and heterogeneous tumors. (genericviabuy.com)
  • In a continuation of our work probing the anticancer activity of small sulindac libraries, we have prepared and screened a small diversity library of α-methyl substituted sulindac amides in the profen class. (nih.gov)
  • Microtubule binding drugs are of special interest as they have important roles in the modulation of cellular functions and many of them act as anticancer agents. (rgcb.res.in)
  • Anticancer drugs: Keeping one step ahead. (jscimedcentral.com)
  • The contribution of synthetic organic chemistry to anticancer drug development. (jscimedcentral.com)
  • Dr. Shen and his team continue to refine and improve upon the MOS technology and to spotlight its versatility, not only as a physiological model for screening potential personalized treatments, but for disease studies and a variety of other applications as well," said Ali Khademhosseini, Ph.D., TIBI's Director and CEO. (technologynetworks.com)
  • Dr. Shen and his team continue to refine and improve the MOS technology and demonstrate its versatility, not only as a physiological model for screening potential personalized treatments, but also for disease studies and a variety of applications of others," said Ali. (genericviabuy.com)
  • Drug delivery systems seem to be a new method for improving drug targeting and release into cells, bringing new opportunities for improving drug efficiency and avoiding potential side effects. (frontiersin.org)
  • Radiosensitizers are chemical agents that have the potential to increase the lethal effect of radicals induced during irradiation [1-6].The extent of radiation damage to cells is dependent on the amount of oxygen available to the cell [1,2,7,8]. (who.int)
  • The RIVM performed drug susceptibility testing by using a modified agar dilution method ( 5 ). (cdc.gov)
  • Functional T-cell assays are exposed to bias at several levels (manufacturing, pre-analytical, analytical and immunological) [ 2 ] and, unfortunately, the lack of a gold standard precludes higher diagnostic accuracy. (ersjournals.com)
  • In the beginning, UPR has protective effect, but in prolonged ER stress UPR triggers apoptotic cell death. (helsinki.fi)
  • Explore how novel tissue staining technologies can be applied to interrogate new drug targets and immune cell while preserving tissue architecture for spatial analysis. (hub-xchange.com)
  • Experimental analysis was also conducted on the characterization ability, drug release, and targeted therapy of nanomedicine. (frontiersin.org)
  • At least in experimental animals, genetical y modified strains, because on the differential effects of a wide greater susceptibility to chemical in these species the interval between variety of carcinogens in humans at carcinogens in utero and during birth and sexual maturity is only a different stages of life, including var early postnatal life is usual y man few weeks. (who.int)
  • Results The relevant literatures contains 184 Chinese medicine prescriptions/proprietary Chinese medicines, 122 flavors of traditional Chinese medicines, and the drug categories were mainly tonic drugs, antipyretic drugs, astringent drugs, and tranquilizers. (bvsalud.org)
  • These tools were initially used in RAS target validation and subsequently for isolating drug leads, guided by the intracellular antibody interaction with RAS. (cancer.gov)
  • We made the hypothesis that the proportion of "bad" responders to mitogen among patients with negative QFT could be underestimated, especially during immunosuppressive drug intake. (ersjournals.com)
  • 1981. Intake and health effects of thallium among a population living in the vicinity of a cement plant emitting thallium containing dust. (cdc.gov)
  • Molecules from animal venoms are promising candidates for the development of new drugs. (nature.com)
  • Billingsley ML. Druggable targets and targeted drugs: enhancing the development of new therapeutics. (jscimedcentral.com)
  • This one-day conference held on 1 July 2008, and sponsored by SCI, the RSC, and ICI brought together scientists from the Republic of Ireland and from Northern Ireland who have varied interests in drug discovery and development and a common interest in synthesis. (soci.org)
  • Professor Maguire underlined the central importance of organic synthesis to the field of chemical biology and the interfaces with drug development and process development. (soci.org)
  • The development of bioinformatics technology has enabled nanomedicine to play a significant role in drug delivery systems. (frontiersin.org)
  • and discovery that sphingosine-1-phosphate receptor-1 (S1P1) is the pharmacologically relevant target of the immunosuppressant drug Fingomilod (FTY720) followed by the development of Siponimod (BAF312), which is currently used for the treatment of multiple sclerosis. (stanford.edu)
  • Characterisation of ER stress in PD models may be important for the current and future drug development of PD. (helsinki.fi)
  • If ER stress is a significant factor that affects the disease development, it would be important to find a drug that alters these mechanisms and UPR. (helsinki.fi)
  • Brad Nelson has twenty five years of experience in leading rapid growth life science organizations, delivering new technology and applications for drug discovery, development and clinical markets. (hub-xchange.com)
  • Joseph Krueger serves as the scientific leader for Invicro's Advanced Pathology Services and is responsible for developing new biomarker applications to meet drug development needs. (hub-xchange.com)
  • Prior to joining Invicro, Joseph was Chief Scientific Officer at Flagship Biosciences where he led the R&D and Scientific efforts in applying novel quantitative tissue image analysis approaches to immuno-oncology and rare diseases to support drug and companion diagnostic development. (hub-xchange.com)
  • Interferons exert their pleiotropic effects through two different receptors. (lookformedical.com)
  • Historic drug susceptibility data was reviewed ( Table ). (cdc.gov)
  • Otherwise, patients receiving at least one immunosuppressive drug (especially steroids) are more likely to have an indeterminate result [ 1 , 4 , 7 , 8 ]. (ersjournals.com)
  • Since in classic chemotherapies it is observed a decrease in immune response, methotrexate (MTX) was used only to compare the PnV effects on innate immune cells with a highly immunosuppressive antineoplastic drug. (nature.com)
  • Studies on embryo toxic effects of thallium using the whole embryo culture technique. (cdc.gov)
  • Medicinal plants used by traditional medical practitioners (TMP) to treat cancers are considered safe when used alone or combined with conventional therapy to ensure their effectiveness and eliminate the toxic effects of orthodox medicines. (who.int)
  • Dr J J Keating (University College Cork) is one of a small group of chemists who synthesise drugs of abuse such as, for example, MDMA (more commonly known as ecstasy) and want the world to know about it! (soci.org)
  • Dr. Shen's team was able to create a MOS atlas of human respiratory and digestive tissues from patient autopsies and infect them with SARS-COV-2 viruses, followed by drug screening to identify drugs that block viral infection and replication within those tissues. (technologynetworks.com)
  • Because we showed that integration neither affects the levels of viral genes, nor those of virally disrupted human genes, a genome-wide screen was performed to identify human genes which expression is influenced by viral integration and have clinical relevance. (cancerindex.org)
  • The team of Dr. Shen was able to create a MOS atlas of human respiratory and digestive tissues from patient autopsies and infect them with SARS-COV-2 viruses, followed by drug screening to identify drugs that block viral infection and replication within those tissues. (genericviabuy.com)
  • Drug repositioning and pharmacophore identification in the discovery of hookworm MIF inhibitors. (jscimedcentral.com)
  • Organic chemistry in drug discovery. (jscimedcentral.com)
  • The role of the medicinal chemist in drug discovery--then and now. (jscimedcentral.com)
  • The latter discovery was important for us because it suggests that the antibody combining sites could generally be accessible for implementing the Abd technology approach to drug discovery. (cancer.gov)
  • Notably, the tuberculin skin test result was negative at screening before she began the TNF-αA treatment, but was now positive (20 mm), suggesting mycobacterial infection. (cdc.gov)
  • Side effects of concern are infectious diseases, which prompt the need for screening for latent mycobacterial infection before commencing treatment ( 7 ). (cdc.gov)
  • Methods The literature related to PMS were collected from China Knowledge Network (CNKI), Wanfang database and Weipu database in the past 20 years, the herbal compound prescriptions for the treatment of PMS were screened and a relevant database were established and analyzed by SPSS. (bvsalud.org)
  • The two-dimensional nano drug carrier has high safety and effectiveness in drug delivery, and its targeted treatment effect on acute T lymphoblastic leukemia cells is obvious. (frontiersin.org)
  • Despite screening, mycobacterial infections have been diagnosed after prolonged treatment in various patients ( 8 ). (cdc.gov)
  • Of course, clandestine synthesis of ecstasy and other drugs of abuse is widespread. (soci.org)
  • Studies in progress are selecting the venom molecules with antitumor and immunomodulatory effects and trying to better understand their mechanisms. (nature.com)
  • However, with an adeq battery of necf expsre blmarkers, prospective studies of en- vironmental effects on pregnancy outcomes might be possible. (cdc.gov)
  • These results demonstrate the importance of DCs in overcoming ICI resistance and achieving effective and durable antitumor responses and, in many cases, antitumor immunity. (izb-online.de)
  • The most effective drugs are fenretinide and α-galactosylceramide (α-GalCer). (frontiersin.org)