Donor cell survival. Medical search. Frequent questions

Hematopoietic stem cell transplantation (HSCT) results in the alteration of several components of the immune system. (medscape.com)
Transplantation can result in granulocytopenia as well as impairment of barrier defenses, cell-mediated immunity, and humoral immunity. (medscape.com)
In autologous transplantation, the donor and recipient is the same individual. (medscape.com)
When the donor is someone other than the recipient, the procedure is described as allogeneic transplantation. (medscape.com)
SEATTLE - Feb. 27, 2013 - In a significant advance for harnessing the immune system to treat leukemias, researchers at Fred Hutchinson Cancer Research Center for the first time have successfully infused large numbers of donor T-cells specific for a key anti-leukemic antigen to prolong survival in high-risk and relapsed leukemia patients after stem cell transplantation. (fredhutch.org)
GVHD is a major complication of stem cell transplantation. (fredhutch.org)
Researchers undertook the clinical trial because relapse remains a leading cause of death after allogeneic hematopoietic cell transplantation for patients with high-risk leukemias. (fredhutch.org)
The outcome for T-cell acute lymphoblastic leukemia (T-ALL) in relapse after hematopoietic stem cell transplantation (HSCT) is quite poor, while, both donor lymphocytes infusion (DLI) and adoptively infusion of γδ T cells in leukemia patients after HSCT have demonstrated good results in prolonging survival time of patients. (psu.edu)
Research Objective Determine optimal islet transplant conditions and systemic treatments that promote graft survival upon transplantation into immune-competent diabetic subjects. (ca.gov)
The stromal cell requirement for androgen-mediated effects was confirmed in vivo by experiments using chimeric animals created by bone marrow transplantation in which androgen receptor expression was restricted to either the stromal or lymphoid cells of the bone marrow. (jci.org)
Relapse and graft- versus -host disease (GvHD) are the main impediments to the clinical success of allogeneic hematopoietic cell transplantation (HCT) in curing malignant blood disorders. (haematologica.org)
We tested this novel hypothesis in over 6,000 HCT from 8/8 HLA-matched UD reported to the European Group for Blood and Marrow Transplantation (EBMT), to demonstrate that HLA-DPB1 non-TPHE mismatches were associated with worse relapse-free survival and overall survival than TPHE mismatches, present in 21.7% of single HLA-DPB1 disparate pairs. (haematologica.org)
Allo-transplantation of islets is limited by availability of donor cells. (ca.gov)
Historically, the most effective treatment for XSCID has been bone marrow transplantation, [i.e. hematopoietic stem cell transplantation (HSCT)], where a patient receives healthy blood-forming cells from a matched sibling donor, or a half-matched parental donor. (stjude.org)
Determine the feasibility and efficacy of a reduced-intensity conditioning regimen comprising alemtuzumab, fludarabine, melphalan, and thiotepa followed by allogeneic peripheral blood stem cell transplantation (PBSCT) in patients with hematologic malignancies. (knowcancer.com)
Allogeneic peripheral blood stem cell (PBSC) transplantation: Patients undergo allogeneic PBSC transplantation on day 0. (knowcancer.com)
After completion of study treatment, patients are followed weekly for 100 days post-transplantation and then periodically for survival. (knowcancer.com)
Allogeneic hematopoietic stem cell transplantation, or HSCT, can cure sickle cell disease. (reachmd.com)
Let's learn more about how and when hematopoietic stem cell transplantation is used in the management of sickle cell disease. (reachmd.com)
So what is a hematopoietic stem cell transplantation? (reachmd.com)
It's also important to think about what patients are appropriate for hematopoietic stem cell transplantation and to understand what some of the donor requirements are. (reachmd.com)
So what are the realities and clinical outcomes associated with hematopoietic stem cell transplantation in sickle cell disease? (reachmd.com)
The success of graft survival after kidney transplantation is closely associated with early graft function based on intraoperative perfusion characteristics of the allograft and good urine output. (intechopen.com)
Allogeneic hemopoietic stem cell transplantation (HSCT) is the only curative option for many patients with hematological malignancies. (nih.gov)
Hence the outcome of reduced intensity HSCT with lymphodepleting antibodies in older patients with intermediate/high-risk hematological malignancies appears comparable to that obtained with fully ablative transplantation in younger patients, even when these older recipients lack HLA-identical sibling donors. (nih.gov)
Survival after allogeneic transplantation depends on donor-recipient matching, the graft-versus-host response and the development of a graft versus leukemia effect. (emory.edu)
This study indicates that transplantation of defined populations of cells can lead to improved patient outcomes," he says, pointing out that it could also have even wider implications. (emory.edu)
The platform technology used in this study can be applied to include additional populations of cells that may further improve survival and decrease complications for patients with leukemia and MDS undergoing allogeneic stem cell transplantation," he says. (emory.edu)
Transplantation of immature cells has been considered a potential therapeutic strategy for the damaged adult brain and spinal cord, and there is currently sustained interest in the generation of stem cell lines that could be used to treat certain CNS injuries or disorders. (hindawi.com)
Although allogeneic hematopoietic cell transplantation (alloHCT) is the treatment of choice, patient eligibility criteria and optimal conditioning regimen remain under study. (confex.com)
Donor Clonal Hematopoiesis and Recipient Outcomes After Transplantation. (harvard.edu)
Among the topics covered are age-related issues with regard to gene expression patterns in kidney transplant recipients and selection of donor-recipient combinations in liver transplantation. (tts.org)
Recent advances in flow cytometric and molecular measurable residual disease detection have further enhanced modern risk-stratified approaches to chemotherapy and allocation to allogeneic hematopoietic stem cell transplantation (HSCT) in first complete remission (CR1) when indicated. (haematologica.org)
Lung transplantation remains the only potential curative option at end-stage disease but is severely limited by a lack of suitable donor lungs and low long-term survival. (lu.se)
Bioengineering lung tissue or bioengineering cells with biomaterials for transplantation is an exciting new approach to (re)generate tissue to close this large unmet clinical need. (lu.se)
They developed the buttonhole technique for anastomosis of donor and recipient vessels in kidney transplantation to prevent thrombus formation. (medscape.com)
The transplantation of organs, cells and tissues has become the treatment of choice for a wide range of both fatal and non-fatal diseases, resulting in high levels of demand for transplantation services, particularly in high- and middle-income countries. (who.int)
In the case of kidney transplantation, the use of organs from live donors produces better results medically than material from deceased donors. (who.int)
Hematopoietic stem cell transplantation is also sometimes used for solid tumors (eg, some germ cell tumors) that respond to chemotherapy. (msdmanuals.com)
Hematopoietic stem cell transplantation may be autologous (using the patient's own cells) or allogeneic (using cells from a donor). (msdmanuals.com)
Peripheral blood has largely replaced bone marrow as a source of stem cells, especially in autologous hematopoietic stem cell transplantation, because stem cell harvest is easier and neutrophil and platelet counts recover faster. (msdmanuals.com)
Umbilical cord hematopoietic stem cell transplantation has been restricted mainly to children because there are too few stem cells in umbilical cord blood for an adult. (msdmanuals.com)
There are no contraindications to autologous hematopoietic stem cell transplantation. (msdmanuals.com)
Allogeneic hematopoietic stem cell transplantation is limited mainly by lack of histocompatible donors. (msdmanuals.com)
The technique for umbilical cord hematopoietic stem cell transplantation is still in its infancy, but it is gaining interest. (msdmanuals.com)
Because cord blood contains immature stem cells, HLA matching appears less crucial than for the other types of hematopoietic stem cell transplantation. (msdmanuals.com)
Kinetics and Risk Factors of Relapse after Allogeneic Stem Cell Transplantation in Children with Leukemia: A Long-Term Follow-Up Single-Center Study. (cdc.gov)
Donor characteristics and hematopoietic stem cell transplantation outcome: experience of a single center in Southern Brazil. (cdc.gov)
The influence of glucocorticoid receptor single nucleotide polymorphisms on outcome after haematopoietic stem cell transplantation. (cdc.gov)
Donor-recipient killer immunoglobulin like receptor (KIR) genotype matching has a protective effect on chronic graft versus host disease and relapse incidence following HLA-identical sibling hematopoietic stem cell transplantation. (cdc.gov)
Chain Predicts Risk of Graft-versus-Host Disease and Cytomegalovirus Infection after Allogeneic Hematopoietic Stem Cell Transplantation. (cdc.gov)
Chronic graft-versus-host disease could ameliorate the impact of adverse somatic mutations in patients with myelodysplastic syndromes and hematopoietic stem cell transplantation. (cdc.gov)
However, an alternative is to perform bone marrow or stem cell tx (transplantation). (lu.se)

Allogeneic transplants are further categorized by the degree of human leukocyte antigen (HLA) match between the donor and recipient. (medscape.com)
1 In unrelated donor (UD)-HCT, frequent human leukocyte antigen (HLA)-DPB1 disparity is the target of T-cell alloreactivity, contributing to both leukemia control and GvHD. (haematologica.org)
Allogeneic HSCT uses human leukocyte antigen (HLA)-matched stem cells derived from a donor. (emory.edu)
A human leukocyte antigen (HLA)-identical sibling donor is ideal, followed by an HLA-matched sibling donor. (msdmanuals.com)

The transplant procedure requires the harvesting of hematopoietic stem cells from a donor. (medscape.com)
Both the stem cells for transplant and the T-cells came from the same matched donors. (fredhutch.org)
All of the patients, who were treated post-transplant at Seattle Cancer Care Alliance , Fred Hutch's site for patient care, received adoptively transferred infusions of billions of enhanced CD8 cytotoxic T-cell clones. (fredhutch.org)
An obstacle to survival is that the beneficial graft-vs.-tumor effect of a transplant can be offset by concurrent GVHD. (fredhutch.org)
His only chance for survival is a bone marrow/stem cell transplant. (giftoflife.org)
Be The Match® connects patients with a matching donor for a life-saving blood stem cell transplant. (bethematch.org)
Immature dendritic cells (imDCs) play an important role in the induction of donor-specific transplant immunotolerance. (newswise.com)
Immature dendritic cells (imDCs) play an important role in the induction of donor-specific transplant immune tolerance. (newswise.com)
Various XSCID gene therapy clinical trials have been conducted over 20 years, both as an alternative to hematopoietic stem cell transplant (HSCT) or following a poor outcome. (stjude.org)
For some people, a stem cell transplant is the only hope of survival. (blood.co.uk)
it can be treated with a stem cell transplant. (blood.co.uk)
When we found out Myla had a match for a stem cell transplant, we were so overwhelmed with relief and hope that we could finally start the process of getting her better. (blood.co.uk)
Myla was given a week of intense chemotherapy before her transplant to get her body ready to receive her donor cells. (blood.co.uk)
Five-year-old Amelia Topa battled the disease on and off throughout her young life before having a stem cell transplant using a newborn baby's umbilical cord blood which gave her a new lease of life. (yahoo.com)
By spring 2018, the family were told a bone marrow transplant using stem cells would be her best chance of survival. (yahoo.com)
An anonymous male donor from America, aged between 16 and 30, donated the stem cells and the transplant went ahead on June 28 2018. (yahoo.com)
Basically, if their doctor tells them their only chance for survival is a stem cell or marrow transplant, they have to find a matching donor. (sfasu.edu)
So the goal is to transplant someone with sickle cell disease before they become too sick, particularly before they develop significant organ damage, such as kidney disease, liver disease, or heart disease, that might even preclude their ability to safely undergo the rigors of a transplant. (reachmd.com)
However, since there are transplant-related risks such as graft versus host disease and life-threatening infection, as well as organ toxicity, one could likely not be transplanted if their sickle cell disease was not under really good control or if they had minimal disease manifestations. (reachmd.com)
And if a full sibling match can't be found, it's also possible to transplant hematopoietic stem cells from a donor who's only a half match. (reachmd.com)
So are there any downsides of undergoing an unrelated donor transplant? (reachmd.com)
Principal investigator Edmund K. Waller, MD, PhD, FACP, says the net result of giving the patient a precise number of donor T cells with specific functions "leads to better elimination of cancer with less graft versus host disease and less toxicity from the transplant. (emory.edu)
A new Phase 3 clinical trial at Winship is enrolling participants after extremely encouraging results in the study's first two phases indicate the first-in-class high-precision cell therapy retains the benefits of stem cell transplant without such serious complications as graft versus host disease (GvHD) and disease relapse in patients with certain blood cancers. (emory.edu)
Waller notes that GvHD is a serious risk for anyone who receives a donor transplant. (emory.edu)
GvHD is an immune reaction of donor T cells against normal tissues in the transplant recipient," he says. (emory.edu)
It can occur in any transplant of blood cells from a donor to a patient and can be very dangerous. (emory.edu)
Orca-T combines purified cells from a matched donor to replace a patient's diseased blood and immune system with a healthy one while lowering their risk of developing GvHD and other life-threatening transplant-related side effects. (emory.edu)
To create the precision-engineered donor graft, Waller explains that different cellular subsets in the graft are selectively purified and then infused into the transplant recipient in a timed fashion. (emory.edu)
In the case of PD, where the progressive and selective loss of dopaminergic neurons within the substantia nigra (SN) leads to dopaminergic denervation of the striatum, one possible solution has been to transplant embryonic nigral cells into the host substantia nigra (SN), in an attempt to rewire nigrostriatal circuit. (hindawi.com)
Future studies aim to engineer donor-compatible beta cells to seed in our biomaterial as a means to avoid transplant rejection and provide long-term restorative insulin therapies," Elizondo said. (endocrine.org)
Transplant Cell Ther 2021. (harvard.edu)
Defibrotide: Real World Management of Veno-Occlusive Disease/ Sinusoidal Obstructive Syndrome after Stem Cell Transplant. (harvard.edu)
A bone marrow (stem cell) transplant may be tried. (medlineplus.gov)
Characteristics and risk of chronic graft-versus-host disease of liver in allogeneic hematopoietic stem cell transplant recipients. (cdc.gov)
Clinical outcomes of HLA-DPB1 mismatches in 10/10 HLA-matched unrelated donor-recipient pairs undergoing allogeneic stem cell transplant. (cdc.gov)

Donors for allogeneic transplants may be related or unrelated to the recipient. (medscape.com)
In syngeneic transplants, the donor is the identical twin of the recipient. (medscape.com)
GvHD is commonly associated with bone marrow transplants and stem cell transplants. (wikipedia.org)
One of the best examples of these two contrasting aspects of T-cell alloreactivity is donorrecipient HLA-DPB1 disparity, present in over 80% of transplants from UD. (haematologica.org)
However, such heterotopic transplants, although capable of alleviating symptoms that benefit from neurotransmitter supplementation, do not re-establish the natural homeostatic regulation of neural activity in the brain and dramatically limit the cell/circuitry replacement strategy to practically only PD. (hindawi.com)
They come to the unit to receive cytostatic treatment or stem cell transplants. (lu.se)
The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)

This suggests that lymphocytes present in engrafted donor stem cells can cause a concurrent therapeutic graft-vs.-tumor effect. (fredhutch.org)
First described in 1975, NK cells were initially identified as a distinct sub-population of lymphocytes by their capacity to spontaneously lyse tumor cells ( 1 ). (frontiersin.org)

The greater the mismatch between donor and recipient, the higher the risk of graft versus host disease (GVHD). (medscape.com)
In GVHD, the donated stem cells view the recipient cells as foreign and attack recipient cells. (medscape.com)
However, because donor stem cells are not selected for specificity for leukemia antigens and commonly recognized proteins expressed by many other host tissues, substantial morbidity and mortality from GVHD can occur. (fredhutch.org)
The scientists theorized that infusions of T-cells that target WT1 could potentially promote additional anti-leukemic activity without inducing GVHD. (fredhutch.org)
Moreover, dynamically monitoring the TCR repertoire in patients who receive HSCT will benefit in supervising of malignant clone evolution and residue, identifying T cell clones mediate anti-infection, GvHD or GvL. (psu.edu)
The white blood cells present within the transplanted tissue then attack the recipient's body's cells, which leads to GvHD. (wikipedia.org)
GvHD occurs when the donor's immune system's white blood cells reject the recipient. (wikipedia.org)
Alloreactive donor T cells are important mediators of both relapse control by graft- versus -leukemia (GvL) effects, and of GvHD. (haematologica.org)
2 We have previously shown that two biological models of HLA-DPB1 mismatching, namely permissiveness according to T-cell epitope (TCE) groups and genetically determined high-expression levels, are individually associated with the risks of non-relapse mortality and GvHD/relapse, respectively. (haematologica.org)
Alloreactive donor T cells recognizing patient-specific genetic polymorphisms, including mismatched HLA allotypes, play a major role in both beneficial GvL and severe GvHD after UD-HCT. (haematologica.org)
Winship study: Can engineered T cell prevent GvHD and relapse in blood cancer? (emory.edu)
These cells expand and help prevent both rejection of the graft and GvHD. (emory.edu)
We have a series of clinical trials available to evaluate several different strategies to prevent GVHD, such as T cell depletion and the use of novel immunosuppressive agents. (harvard.edu)

This year, it's all of those things in addition to COVID-19 restrictions and overall health and safety concerns for potential donors and ASBP blood donor center team members. (health.mil)
We work with a global network of international registries, which collectively have over 28 million potential donors, to make this possible. (blood.co.uk)
Brian Allison, community engagement representative for Be The Match, was on site helping educate potential donors. (sfasu.edu)

This review describes current considerations in the use of hydroxyurea for the management of sickle cell disease in the context of clinical severity. (cdc.gov)
Well, it's transplanting blood stem cells from a donor without sickle cell disease into a recipient who does have sickle cell disease. (reachmd.com)
The blood cells are harvested from the donor and stored, and then the sickle cell disease patient is given high doses of chemotherapy to get rid of all the remaining blood cells in the bone marrow in order for them to be able to receive the healthy donated cells. (reachmd.com)
This essentially cures the patient of sickle cell disease. (reachmd.com)
But unfortunately, only about 20% of patients with sickle cell disease in the United States have a matched donor. (reachmd.com)
The main requirement for a donor is to not have sickle cell disease or infection, such as HIV or hepatitis C. But what's important to know is that it's perfectly fine for the donor to have sickle cell trait. (reachmd.com)
ABSTRACT Introduction: The perception of prejudice against, and stigmatization of, sickle cell disease (SCD) leads the patient to perceive a different treatment, due to the disease stigma and maybe related to a worse quality of life (QoL). (scielo.br)
Objectives: Describe and evaluate the perception of the prejudice against the disease and its impact on the quality of life of patients with sickle cell disease. (scielo.br)
However, in sickle cell disease (SCD) primarily due to repeated blood transfusions and hemolysis-induced recycling of iron, its supplementation during pregnancy remains questionable and may be harmful. (scielo.br)

Here, we reported a T-ALL case who experienced three relapses and received HSCT and DLI with an overall survival (OS) time lasting for more than seven years. (psu.edu)
These results indicate that clonally expanded Vd4 T cells may represent a reconstituted γδ T cell repertoire after HSCT, which also hints to a relatively better outcome for this case. (psu.edu)
T-cell immunity may also decrease over time, requiring 26% of patients to undergo a second HSCT. (stjude.org)
Since many of these patients lack HLA-identical sibling donors and are older or have comorbidity, a fully ablative HSCT is not feasible and an alternative approach is required. (nih.gov)

In preparation for receipt of the stem cells, recipients undergo myeloablation to eliminate their own myeloid cells. (medscape.com)
The survival span of C57bl/6 skin grafts was significantly prolonged in immunized Balb/c recipients with a donor-specific pattern. (newswise.com)

The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
eg, bone, bone marrow, and skin grafts) Genetically identical (syngeneic [between monozygotic twins]) donor tissue (isografts) Genetically. (msdmanuals.com)

The stem cell source may be bone marrow, peripheral blood, or umbilical cord blood. (medscape.com)
Castration of normal male mice induces expansion of the bone marrow B cell population, an effect that can be reversed by androgen replacement. (jci.org)
Immature B cells from normal mouse bone marrow were not responsive to the suppressive effect of androgens unless they were cocultured with marrow stromal cells or with supernatants from androgen-treated stromal cells, suggesting that the androgen effects are exerted through marrow stromal elements by production of a diffusible mediator. (jci.org)
Further experiments revealed that bone marrow stromal cells produced TGF-β in response to dihydrotestosterone (DHT), and neutralization of TGF-β in the DHT-treated stromal cells reversed the suppressive effects. (jci.org)
These experiments suggest that effects of androgens on developing B cells are mediated through androgen receptors in bone marrow stromal cells. (jci.org)
Several lines of evidence support the hypothesis that gonadal steroids exert effects on the development of B cells in bone marrow. (jci.org)
Androgens also exert effects on B cell development in bone marrow. (jci.org)
Approximately 100 SFA students in courses taught by Patty Berthot, SFA lecturer in the Department of Kinesiology and Health Science, and many student-athletes worked to promote the drive and encourage others to become bone marrow donors. (sfasu.edu)
The cells are remarkable in that they can find their way into the recipient's bone marrow, where they begin to divide and repopulate the patient's bone marrow as his or her own. (reachmd.com)
First are the bone-marrow-reconstituting stem cells and a population of regulatory T cells. (emory.edu)
People with AML have many abnormal immature white blood cells inside their bone marrow. (medlineplus.gov)
Bone marrow aspiration and biopsy will show if there are any leukemia cells. (medlineplus.gov)

Physicians have also had success using umbilical cord blood, which is rich in stem cells. (stjude.org)
Doctors made the decision to have her undergo a second round of stem cell treatments, this time from an umbilical cord. (yahoo.com)
Other donor cells can come from unrelated match donors, partially matched or half-matched family members, or in some circumstances, from an umbilical cord blood donor. (reachmd.com)

For many patients, their only chance of survival is to find a matching donor as quickly as possible. (bethematch.org)
See the range of financial gift options to help more patients find a matching donor, help with uninsured costs and fund life-saving research. (bethematch.org)

No HLA-matched (10/10 or 9/10) sibling donor or unrelated donor available NOTE: A new classification scheme for adult non-Hodgkin's lymphoma has been adopted by PDQ. (knowcancer.com)

We urgently need your help to find his lifesaving marrow or stem cell donor. (giftoflife.org)

The harvested stem cells are processed before infusion into the recipient. (medscape.com)
In addition, an identical Vd4 monoclonal T cell that proliferated in the recipient for several years was confirmed to come from the donor graft, and its expression level significantly increased in third leukemia recurrence. (psu.edu)
White blood cells of the donor's immune system which remain within the donated tissue (the graft) recognize the recipient (the host) as foreign (non-self). (wikipedia.org)
Association of Blood Donor Age and Sex With Recipient Survival After Red Blood Cell Transfusion. (ohri.ca)

Newer studies with intensive multiagent chemotherapy (eg, the CALGB [Cancer and Leukemia Group B]- 8811 and hyper-CVAD [hyperfractionated cyclophosphamide, vincristine, doxorubicin, and dexamethasone] regimens and the ALL-2 regimen) plus the addition of TKIs for Ph+ ALL and rituximab for CD20-positive ALL have resulted in 3-y survivals of 50% or more in adults. (medscape.com)
The following factors were studied in the whole cohort: age, type of disease (treatment-related or post myelodysplastic syndrome/MDS), previous intensive chemotherapy cycles, cytogenetic risk, overall (OS) and disease-free survival (DFS). (confex.com)
1-4 These survival gains have been attributed largely to advances in biological and genetic characterization of heterogeneous pediatric AML subtypes via next-generation sequencing with clinical outcome correlation, and to enhanced supportive care measures focused on reducing toxicities from intensive multi-agent chemotherapy regimens required for cure. (haematologica.org)
Treatment involves using medicines ( chemotherapy ) to kill the cancer cells. (medlineplus.gov)
Chemotherapy kills normal cells, too. (medlineplus.gov)

Resource utilization and toxicities after carboplatin/etoposide/melphalan and busulfan/melphalan for autologous stem cell rescue in high-risk neuroblastoma using a national administrative database. (chop.edu)
The overall aim of this thesis has been to assess the potential of autologous grafting in cell replacement therapy for PD. (lu.se)
Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)

Ours is also the first report to show that greatly improved T-cell in-vivo persistence can be achieved after transfer by modifying the way cells are generated in tissue culture for therapy with inclusion of the cytokine IL-21," said Aude Chapuis, M.D., lead author on the study and a research associate in the Fred Hutch Immunology Program. (fredhutch.org)
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
Background: Stem cells are of two types: embryonic and adult stem cells and they act as a repair system by replenishing body tissue. (benthamscience.com)
Discussion: Activation of the PI3K/AKT pathway decreases the concentration of apoptotic factors, while the HIF pathway protects stem cells against the micro-environment of tissue (hypoxia). (benthamscience.com)
Ramalingam, M. Surface functionalization of nanobiomaterials for application in stem cell culture, tissue engineering, and regenerative medicine. (benthamscience.com)
Recent research has shown that pieces of fetal nigral tissue placed in the striatum of 6-OHDA lesioned rats offer greater cell survival and predictability of graft function (in comparison to dissociated nigral cells) in the animal model of Parkinson's disease [ 20 ]. (hindawi.com)
NK cell infiltration into tumor tissue is associated with better disease prognosis in colorectal cancer, clear cell renal cell carcinoma, and lung carcinomas ( 9 - 11 ). (frontiersin.org)
This is the soft tissue in the center of bones that helps form all blood cells. (medlineplus.gov)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

Cell therapies produced from the UDC line will not be rejected by a patient's immune system. (ca.gov)
Research Objective Development of hESC-derived pancreatic beta cells that are protected from allogeneic and autoimmune attack into a cell therapy for type 1 diabetes (T1D) Impact Cell therapy of T1D is challenged by immune rejection. (ca.gov)
Regulation of the immune system via modulation of intestinal microbes is partially explained by observations that certain bacteria activate specific types of immune cells. (nature.com)
This study focused on exploring sinomenine (SN) to promote differentiation of induced pluripotent stem cells (iPSCs) into imDCs (SN-iPSCs-imDCs), and found SN-iPSCs-imDCs with worse donor-specific T-cell stimulatory function, and higher regulatory T-cell proliferative function in vitro and in vivo to induce high immune tolerance. (newswise.com)
Now, more than 2 years after undergoing this new gene therapy, initial patients are producing a greater percentage of immune cells, including T, B and natural killer cells. (stjude.org)
In 3 younger patients, similar levels of gene-modified immune cells were also observed at 6 to 9 months following treatment. (stjude.org)
In people with type 1 diabetes, beta cells are attacked and destroyed by the immune system. (endocrine.org)
Implanting the beta cells using the biomaterial did not trigger an immune response. (endocrine.org)
While transplanting donor beta cells is one promising approach to combat type 1 diabetes in the future, the immune system response that destroys a person's own cells in the first place can happen again and attack the transplanted cells. (endocrine.org)
In the early phases of cancer immune surveillance, NK cells directly identify and lyse cancer cells. (frontiersin.org)
Here, we review tumor-NK cell interactions, discuss the mechanisms by which NK cells generate an antitumor immune response, and discuss NK cell-based therapeutic strategies targeting activating, inhibitory, and co-stimulatory receptors. (frontiersin.org)
instead of targeting tumor cells, the goal of immunotherapy is to augment and expand the immune system's intrinsic antitumor response. (frontiersin.org)
However, a class of important immune-modulators is conspicuously absent: agents that utilize the power of innate immune cells to eradicate tumors. (frontiersin.org)
An important class of innate immune cells that play a critical role in mediating the antitumor immune response is the natural killer (NK) cell. (frontiersin.org)
NK cells are now accepted to play an important role in both the adaptive and innate immune responses that govern infection, autoimmunity, and tumor immunosurveillance ( 2 , 3 ). (frontiersin.org)
One concern about the procedure is that immune cells in cord blood do not have experience with the viruses responsible for latent infections, leading to a higher percentage of naive T cells and thus increased vulnerability to reactivation of cytomegalovirus or Epstein-Barr virus infection. (msdmanuals.com)

Reporting results of a pilot clinical trial in the Feb. 27 issue of the journal Science Translational Medicine , researchers describe the use of T-cells that were taken from a donor, programmed in the lab to recognize the Wilm's Tumor Antigen 1 (WT1) and kill leukemia cells, grown in large numbers, and then infused into patients to promote anti-leukemic activity. (fredhutch.org)
Heterogeneous antigen expression is a key barrier influencing the activity of chimeric antigen receptor (CAR) T cells in solid tumors. (nature.com)
Here, we develop CAR T cells targeting glypican-1 (GPC1), an oncofetal antigen expressed in pancreatic cancer. (nature.com)
We find that a structurally rigid IgG4H and CD28TM domain brings the two D4 fragments in proximity, driving CAR dimerization and leading to enhanced T-cell signaling and tumor regression in pancreatic cancer models with low antigen density in female mice. (nature.com)
CAR T-cell potency is highly dependent on target antigen expression, and CARs often fail to exert their antitumor activities when antigen expression is low or below a certain threshold. (nature.com)
Furthermore, by investigating the impact of hinge (CD8 and IgG4) and TM (CD8 and CD28) on D4 CAR T cells, we discover that IgG4H-CD28TM mediated D4 CAR dimerization, leading to enhanced T-cell signaling and tumor regression in pancreatic cancer models with low antigen density. (nature.com)

They also discovered that these mutated genes coincided with the abnormal expansion of the blood cells that harbor them - a phenomenon known as clonal hematopoiesis (CH) . (dana-farber.org)

The first thing you need to do is to identify a suitable donor, and that's most commonly an HLA identical sibling. (reachmd.com)
Since 2013, we have introduced FluTreo for patients with a suitable donor that would have been previously eligible only for reduced intensity conditioning/RIC. (confex.com)

WT1 is expressed 10 to 1,000 times higher in leukemic cells compared to normal CD34 blood stem cells, and the magnitude of expression correlates with the aggressiveness of acute myeloid leukemia, acute lymphoid leukemia and myelodysplastic syndromes. (fredhutch.org)
Our work provides a synthesis of previous algorithms, mechanistically based on HLA-DPB1 immunopeptidome divergence 5 and expression by residual leukemia cells, 6 , 7 respectively, into a new and integrative model for intelligent mismatching in UD-HCT, to improve survival for future patients. (haematologica.org)
We determined the capacity of the leukemia cell line K562 modified to express a membrane-bound form of interleukin (IL)-15 and 41BB ligand (K562-mb15-41BBL) to generate human NK cells with enhanced cytotoxicity. (aacrjournals.org)
The expanded NK cells were significantly more potent than unstimulated or IL-2-stimulated NK cells against acute myeloid leukemia cells in vitro . (aacrjournals.org)
Winship is one of several clinical trial sites for the Precision-T study evaluating the safety and efficacy of Orca-T, a cell therapy that combines purified cells from a matched donor, in patients with acute myeloid leukemia (AML), acute lymphocytic leukemia (ALL) and high-risk myelodysplastic sydromes (MDS). (emory.edu)
The conventional T cells help eliminate the cancer in the patient through a process called "graft-versus-leukemia. (emory.edu)
Subtypes are based on specific changes in genes (mutations) and how the leukemia cells appear under the microscope. (medlineplus.gov)

The best results were achieved when some of the patients received T-cell clones that were exposed to interleukin 21 (IL-21) during the programming and growth process, based on the hypothesis that such exposure would create cells that could survive longer and produce greater anti-leukemic activity after transfer. (fredhutch.org)
This is the first time patients have received an infusion of WT1 specific T-cells, and thus also the first demonstration that such cells can provide a therapeutic anti-leukemic effect, as has been suggested from earlier vaccine trials that induce less potent responses," said Philip Greenberg, M.D. , corresponding author and head of the Immunology Program at Fred Hutch. (fredhutch.org)
The findings support expanding efforts to target WT1 and provide insights into what is necessary to establish potent and persistent T-cell responses in patients, and such second generation studies have recently been initiated at Fred Hutch. (fredhutch.org)
Four of the 11 patients in the trial received infusions of T-cells that targeted WT1 and were generated in the presence of IL-21. (fredhutch.org)
Among the seven patients who received infused T-cells generated without the presence of IL-21, two showed direct evidence of anti-leukemic activity, including one patient with advanced progressive disease who had a temporary response. (fredhutch.org)
Impact Providing the immediate cell therapeutic candidate for clinical trial of diabetic patients. (ca.gov)
It is less common in younger patients and in those with closer human leukocyte antigens (HLA) matches between donor and the patient. (wikipedia.org)
Let's score a touchdown for patients in need of a donor. (bethematch.org)
Our registry is the connection between patients searching for a cure and their life-saving blood stem cell or marrow donor. (bethematch.org)
Become a potential life-saver to patients battling blood cancers or blood diseases in need of a donor. (bethematch.org)
The vector was made on our campus by our GMP facility that produces clinical grade biological products, which also re-engineers the patients' cells to carry the healthy new gene. (stjude.org)
Results for 8 patients under the age of 2 with XSCID in the second trial were presented at the 21st Annual Meeting of the American Society of Gene & Cell Therapy in May 2018. (stjude.org)
But matching donors and patients isn't easy. (blood.co.uk)
Determine the safety of LMB-2 immunotoxin-treated, selectively-depleted donor T cells, administered after allogeneic PBSCT, in these patients. (knowcancer.com)
T cell preparation: Patients and donors undergo apheresis to obtain peripheral blood mononuclear cells (PBMCs), which are expanded in culture. (knowcancer.com)
Patients' PBMCs are irradiated and mixed with donor PBMCs. (knowcancer.com)
LMB-2 immunotoxin-treated, selectively-depleted donor T cells: Patients receive LMB-2 immunotoxin-treated, selectively-depleted donor T cells IV over 30-60 minutes on approximately day 28. (knowcancer.com)
Cohorts of 3-6 patients receive escalating dose of LMB-2 immunotoxin-treated, selectively-depleted donor T cells until the maximum tolerated dose (MTD) is determined. (knowcancer.com)
Imlifidase is a cysteine protease that cleaves IgG leading to a rapid decrease in antibody level and is shown to convert a positive crossmatch test to negative, thus allowing highly sensitized patients to be transplanted with a living or deceased donor kidney. (tts.org)
We hypothesized that patients transplanted with FluTreo would have a long-term survival advantage over other treatment alternatives. (confex.com)
While these measures have improved event-free survival (EFS) for children and adolescents/young adults with de novo AML, 30-40% of patients ultimately relapse. (haematologica.org)
[ 3 ] Porcine islet cells of Langerhans have been injected into patients with type 1 diabetes mellitus . (medscape.com)
[ 5 ] and pig neuronal cells have been transplanted into patients with Parkinson (Parkinson's) disease and Huntington (Huntington's) disease . (medscape.com)
Because only one fourth of patients have such a sibling donor, mismatched related or matched unrelated donors (identified through international registries) are often used. (msdmanuals.com)
The object of the statistical analysis is to compare the actual survival among the patients with the hypothetical counter-factual survival that would have occurred if no tx had been performed. (lu.se)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)

Association Between Donor-Specific Antibodies and Acute Reje. (lww.com)
Donor-specific antibodies (DSA) are associated with acute kidney graft rejection, but their role in small bowel/multivisceral allograft remains unclear. (lww.com)

Elan's is a complicated case as he has other genetic abnormalities including GATA 2, monosomy 7, and MSH6 deficiency further enhancing the dire need for stem cells. (giftoflife.org)
The Precision-T study is funded by Orca Bio, a clinical-stage biotechnology company developing purified, high-precision cell therapies for the treatment of cancer, autoimmune diseases and genetic blood disorders. (emory.edu)
How well you do depends on your overall health and the genetic subtype of the AML cells. (medlineplus.gov)

All four survived after T-cell therapy without relapse for more than 30 months without suffering graft-vs.-host-disease and required no additional anti-leukemic treatment, according to the study. (fredhutch.org)
Based on our previous identification of a leukemic and reactive clone in this patient, continual γδ T cell repertoire monitoring affirmed that the same Vd5 leukemic clone existed in most samples from the patient, particularly including a sample taken at the time of the third T-ALL relapse, while it could not be detected in the donor sample. (psu.edu)

The appearance of moderate to severe cases of cGVHD adversely influences long-term survival. (wikipedia.org)

The EasySep™ Release Human PE Positive Selection Kit is designed to isolate cells that are labeled with PE-conjugated antibodies from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection. (stemcell.com)
Additionally, a 11-year prospective cohort study of Japanese inhabitants linked low peripheral-blood NK cell cytotoxicity with increased cancer risk ( 12 ). (frontiersin.org)

Research Objective We will produce a universal donor cell (UDC) line by gene editing an embryonic stem cell line. (ca.gov)

However, long-term disease-free survival rates may be lower than those with HLA-identical sibling donors. (msdmanuals.com)

With a median follow-up of 715 (216-1470) days, nonrelapse mortality, actuarial event-free and overall survival is 27, 45 and 45%, respectively. (nih.gov)

Research Objective The expected outcome is a therapeutic candidate, allogeneic HSC-engineered HLA-I/II-negative human iNKT cells, that can potentially be used as an off-the-shelf cellular therapy for treating cancer. (ca.gov)

We demonstrate that replacing a lengthy CD8H with a short IgG4H significantly improves D4 CAR T-cell activity. (nature.com)
Now the question arises whether such tx improves the survival probability, and an ongoing study is hoped to answer that question. (lu.se)

Cell Therapy for Parkinson's Disease: What Next? (lu.se)

Hematology, transfusion and cell therapy 2018 7 40 (2): 136-142. (cdc.gov)

Melton, D.A. "Stemness": Transcriptional profiling of embryonic and adult stem cells. (benthamscience.com)
Indigenous cells rarely regenerate across the adult CNS, and neural circuitry reconstruction is made difficult by the fact that the adult CNS expresses molecules, that inhibit axonal growth, and/or fails to express precise gradients of growth-promoting cues that would stimulate/direct axonal growth [ 1 - 4 ]. (hindawi.com)
Past studies have shown that some fetal nigral cells transplanted in this way can grow a limited number of axonal projections towards the striatum, but most are generally incapable of growing over the distance required to establish functional connections in the striatum in the adult brain [ 8 - 10 ]. (hindawi.com)
Xenografts have been proposed as appropriate for infants who are physically too small to accommodate organs retrieved from adult or pediatric donors. (medscape.com)

The development of clinically applicable methods to produce large numbers of fully functional NK cells is a critical step to maximize the potential of this approach. (aacrjournals.org)
The goal is to maximize the survival of transferred fat so that each cell has access to the blood supply. (drpautler.com)
To maximize donations from deceased donors, an effective coordinating organization, adequate medical and logistic infrastructure, appropriately trained staff and governmental involvement are needed. (who.int)

Therapeutic intervention aims to reverse tumor-induced NK cell suppression and sustain NK cells' tumorlytic capacities. (frontiersin.org)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)

Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
Hydroxyurea has proven clinical efficacy for SCA - treatment significantly reduces disease manifestations and prolongs survival. (cdc.gov)
Implantation of the beta cell-seeded biomaterial reversed diabetes in a mouse model by effectively normalizing glucose levels and significantly increasing survival. (endocrine.org)

Sickle cell anemia, which primarily affects Black or African American people, is associated with a shorter life span and life-threatening complications that can affect all parts of the body. (cdc.gov)

Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)

Anatomy30

Organisms19

Diseases24

Chemicals and Drugs48

Analytical, Diagnostic and Therapeutic Techniques and Equipment73

Phenomena and Processes58

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science5

Named Groups3

Health Care16

Transplantation53

Human leukocy4

Transplant35

Transplants8

Lymphocytes2

GVHD13

Potential donors3

Sickle Cell Di9

HSCT4

Recipients2

Genetically2

Bone12

Umbilical3

Find a match2

Unrelated donor available1

Stem cell donor1

Recipient4

Chemotherapy5

Autologous3

Tissue16

Immune16

Antigen6

Clonal hematopoiesis1

Suitable2

Leukemia7

Patients26

Acute2

Genetic3

Relapse2

Long-term survival1

Peripheral2

Embryonic stem1

Identical1

Overall survival1

Outcome1

Improves2

Therapy for Parkinson's Disease1

Transfusion and Cell Therapy1

Adult4

Maximize3

Therapeutic2

Significantly3

Complications1

Progenitors2