Domains transcriptional activation. Medical search. Frequent questions

TSC2 contains a GTPase Activating Protein (GAP) domain which has been shown to stimulate (down regulate) the GTPase activity of the small GTPase Rheb, which is - in its GTP bound form - an activator of mTORC1. (wikipedia.org)
Finally, we report new activator domains, including a divergent KRAB. (stanford.edu)
Transactivation assay in yeast cells revealed that CmNAC1 functions as a transcription activator, and its transactivation domain is located in the C-terminus. (frontiersin.org)
GmNAC11 is a transcriptional activator, whereas GmNAC20 is a mild transcriptional repressor. (frontiersin.org)
Thus, the ability for HER3 to function as a transcriptional co-activator may be dependent on specific C-terminal TADs. (plos.org)
therefore, p50 and p52 homodimers repress transcription unless they are bound to a protein containing a TAD, such as RelA, c-Rel or RelB or Bcl-3 (a related transcriptional co-activator). (abcam.com)
Priyadarshini Y., Natarajan, K. (2016) Reconfiguration of transcriptional control of lysine biosynthesis in Candida albicans involves a central role for the Gcn4 transcriptional activator. (jnu.ac.in)
Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. (nih.gov)
Interphase movements of a DNA chromosome region modulated by VP16 transcriptional activator. (ad-astra.ro)
The human Brm protein has been reported to be a 180 kDa nuclear factor that acts as a transcriptional activator when fused to a heterologus DNA binding domain. (bdbiosciences.com)
Known classically to function as a transcriptional co-activator, YAP interacts with TEA domain transcription factors (TEAD1-4) to induce expression of target genes. (cheric.org)
Pax5 (BSAP) is essential for B cell development and acts both as a transcriptional activator and a repressor. (elsevierpure.com)
These data suggest that Daxx can affect Pax5's roles as an activator or repressor in B cells and describe a role for Daxx as a transcriptional coactivator. (elsevierpure.com)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)

Finally, by overexpressing TEAD4 in yes-associated protein ( YAP )/transcriptional coactivator with PDZ binding motif ( TAZ )-knockdown HCC cells, we showed that the effect of TEAD4 on cell proliferation and its regulation of HSP70 expression does not require YAP and TAZ, the main effectors of the Hippo signaling pathway. (nih.gov)
Lis, J. Promoter-associated pausing in promoter architecture and postinitiation transcriptional regulation. (nature.com)
As a largest plant-specific transcription factor family, NAC domain proteins play an important role in plant development and regulation of abiotic stress tolerance. (frontiersin.org)
The NAC domain transcription factors in Arabidopsis function in plant development, senescence, and stress regulation. (frontiersin.org)
HMG-box domains are found in one or more copies in HMG-box proteins, which form a large, diverse family involved in the regulation of DNA-dependent processes such as transcription, replication, and strand repair, all of which require the bending and unwinding of chromatin. (embl.de)
Singh, R.P., Prasad, H.K., Sinha, I., Agarwal, N. and Natarajan, K. (2011) Cap2-HAP Complex Is a Critical Transcriptional Regulator That Has Dual but Contrasting Roles in Regulation of Iron Homeostasis in Candida albicans. (jnu.ac.in)
Research efforts to date have focused on identifying how either components of the phosphoenolpyruvate-phosphotransferase system (PTS) or global transcriptional networks affect the regulation of virulence factors, but not the synergistic relationship between the two. (umd.edu)
We also made key contributions to identifying the eIF2α kinase Gcn2 and elucidating its regulation by amino acids, via allosteric control of kinase activity by uncharged tRNAs and Gcn2 regulatory domains, and by the TOR pathway through Gcn2-Ser577 phosphorylation. (nih.gov)
Several of the SNF and SWI genes of Saccharomyces cerevisiae encode proteins that are involved in the regulation of transcriptional activation. (bdbiosciences.com)
Tumor-infiltrated macrophages were isolated from human GC tissues using magnetic beads, gene transcription was determined by real-time PCR, protein expression was monitored using western blots, metabolites were determined using HPLC, and transcriptional regulation was analyzed by the luciferase-based reporter gene system. (cancerindex.org)
On the other hand, WW domains of YAP are indispensable for the regulation of TP73 expression, which may recruit YAP to TP73 gene though interaction with ZEB1 and/or RUNX2, two transcriptional repressors. (cheric.org)
Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)
Activation of Btk results in a cascade of signaling events resulting in calcium mobilization and fluxes, cytoskeletal rearrangements, and transcriptional regulation involving nuclear factor-kappaB (NF-kappaB) and nuclear factor of activated T cells (NFAT). (medscape.com)

Transcriptional enhancer factor domain family member 4 (TEAD4) is a downstream effector of the conserved Hippo signaling pathway, regulating the expression of genes involved in cell proliferation and differentiation. (nih.gov)
Although pausing has been connected to extremely rapid and synchronous activation of genes, pausing is also highly associated with constitutively expressed genes that encode signalling and transcription factors. (nature.com)
Distinct signals that act through diverse targeted transcription factors can regulate different steps in the transcription pathway and provide a highly modulated transcriptional response at individual genes. (nature.com)
Inside the nucleus, HER family members (EGFR, HER2, and HER3) have been shown to function as co-transcriptional activators for various cancer-promoting genes. (plos.org)
The Horizon CRISPRmod CRISPR activation (CRISPRa) system is an adapted CRISPR-Cas9 system that is used for upregulation of genes. (horizondiscovery.com)
The DNA-binding domain (DBD) of the hGRα corresponds to amino acids 420-480 and contains 2 zinc finger motifs through which the hGRα binds to specific DNA sequences, the glucocorticoid-response elements (GREs) in the promoter region(s) of target genes. (medscape.com)
We are analyzing the physiological role of several genes implicated in different aspects of stem cell activation, self-renewal, and differentiation. (ad-astra.ro)
Muchardt C, Yaniv M. A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor. (bdbiosciences.com)
However, a number of genes are repressed upon YAP activation, suggesting a transcriptional repressor role of YAP. (cheric.org)
We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)
We also synthesize a library consisting of 70,290 guides targeting all human RefSeq coding isoforms to screen for genes which, upon activation, confer resistance to a BRAF inhibitor. (cdc.gov)

We find a relationship between repressor function and evolutionary age for the KRAB domains, discover that Homeodomain repressor strength is collinear with Hox genetic organization, and identify activities for several domains of unknown function. (stanford.edu)

A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. (umu.se)
The non-canonical pathway is responsible for the activation of p100/RelB complexes and occurs during the development of lymphoid organs responsible for the generation of B and T lymphocytes (Figure 2). (abcam.com)
Sinha I, Poonia P, Sawhney S, Natarajan K. (2017) Functional Specialization of Two Paralogous TAF12 Variants by Their Selective Association with SAGA and TFIID Transcriptional Regulatory Complexes. (jnu.ac.in)
Our group discovered the Gcn2 positive effectors Gcn1 and Gcn20 and the importance of ribosomal Gcn1/Gcn20/Gcn2 regulatory complexes in Gcn2 activation. (nih.gov)

One mechanism underlying CRPC and resistance to hormonal therapies is the expression of constitutively active splice variant(s) of androgen receptor (AR-Vs) that lack its C-terminus ligand-binding domain. (bcgsc.ca)
The human GR is a modular protein composed of distinct regions illustrated in panel B in the image below, as follows: (1) The amino-terminal A/B region, also called immunogenic or N-terminal domain (NTD) and (2) the C, D, and E regions, which correspond to the DNA-binding domain, the hinge region, and the ligand-binding domain, respectively. (medscape.com)

Mutations in the major core histones relieve transcriptional repression and bypass the requirement for SNF/SWI and SAGA. (sdbonline.org)

These results provide a resource of 600 human proteins containing effectors and demonstrate a scalable strategy for assigning functions to protein domains. (stanford.edu)
In addition, NAC domain proteins have become a popular research topic for different plant species. (frontiersin.org)
There are 46627 HMG domains in 39088 proteins in SMART's nrdb database. (embl.de)
Taxonomic distribution of proteins containing HMG domain. (embl.de)
The complete taxonomic breakdown of all proteins with HMG domain is also avaliable . (embl.de)
Click on the protein counts, or double click on taxonomic names to display all proteins containing HMG domain in the selected taxonomic class. (embl.de)
Furthermore, AT-hook motifs are frequently associated with known functional domains seen in chromatin proteins and in DNA-binding proteins (e.g. histone folds, homeodomains and zinc fingers). (embl.de)
HMG domain proteins: architectural elements in the assembly of nucleoprotein structures. (embl.de)
The HMG family of proteins comprises members with multiple HMG domains that bind DNA with low sequence specificity, and members with single HMG domains that recognize specific nucleotide sequences. (embl.de)
There are 52668 BROMO domains in 38864 proteins in SMART's nrdb database. (embl-heidelberg.de)
Nearly all known histone-acetyltransferase (HAT)-associated transcriptional co-activators contain bromodomains, which are approximately 110-amino-acid modules found in many chromatin-associated proteins. (embl-heidelberg.de)
In the absence of Wnt signaling, we found that Tcf factors associate with proteins of the Groucho family of transcriptional repressors to repress target gene transcription (9). (hubrecht.eu)
Its activation is tightly controlled by numerous other signaling proteins including protein kinase C (PKC), Sab/SH3BP, and caveolin-1. (medscape.com)

High throughput measurements of direct activation domain-coactivator interactions. (stanford.edu)

GmNAC11 and GmNAC20 from soybean are induced by phytohormones and abiotic stress with different transcriptional activities. (frontiersin.org)

Transfected Brm, expressed in cells lacking endogenous protein, can cooperate with the glucocorticoid receptor (GR) in transcriptional activation. (bdbiosciences.com)
Upon transient transfection assay of Daxx in B cells expressing endogenous Daxx and Pax5, we observed not only transcriptional corepression but also, unexpectedly, coactivation in M12.4.1 and A20 mouse B cell lines. (elsevierpure.com)
We describe structure-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci. (cdc.gov)

Gβ2 was associated with GR/glucocorticoid response elements (GREs) in vivo and suppressed activation function-2-directed transcriptional activity of the GR. We conclude that the Gβγ complex interacts with the GR and suppresses its transcriptional activity by associating with the transcriptional complex formed on GR-responsive promoters. (rupress.org)
Singh P, Coe J, Hong W. A role for retinoblastoma protein in potentiating transcriptional activation by the glucocorticoid receptor. (bdbiosciences.com)

To understand how the B 1 and B 2 regions influenced the transcriptional functions of nuclear HER3, we performed cyclin D1 promoter-luciferase assays in which HER3 deleted of the B 1 and B 2 regions was severely hindered in regulating this promoter. (plos.org)
TAF9 C-terminal conserved region domain is required for SAGA and TFIID promoter occupancy to promote transcriptional activation. (jnu.ac.in)
Activation of the ICP0 promoter was observed when protein synthesis was blocked by alternative agents. (microbiologyresearch.org)
Tiling a given promoter region with several sgRNAs can produce more robust transcriptional activation 9 - 11 , but this requirement presents enormous challenges for scalability, and in particular for establishing pooled, genome-wide GOF screens. (cdc.gov)
activation is via the proximal promoter. (lu.se)

Transcriptional Profiling Shows that Gcn4p is a Master Regulator of Gene Expression During Amino Acid Starvation in Yeast. (jnu.ac.in)
While it is known that the PTS can affect activity of the Mga virulence regulator, further characterization of the mechanism by which sugars and its protein domains affect activity have not been studied. (umd.edu)
Global transcriptional regulator downstream RpfR (GtrR) is a key downstream regulator for quorum-sensing signaling molecule cis-2-dodecenoic acid (BDSF). (ntu.edu.sg)

The current study aimed to map the putative transactivation domains (TADs) of the HER3 receptor. (plos.org)
RelA, c-Rel, and RelB contain C-terminal transcriptional activation domains (TADs), which enable them to activate target gene expression. (abcam.com)

and exerts a dominant negative effect on the transcriptional activity of hGRα. (medscape.com)

The inhibition of Pin1 expression or its activity selectively reduced the transcriptional activities of full-length AR and AR-V7. (bcgsc.ca)
Blocking androgen receptor (AR) transcriptional activity by androgen deprivation therapy (ADT) improves the response to radiotherapy for intermediate and high risk prostate cancer. (bcgsc.ca)
Transcript levels of the androgen receptor (AR)-regulated gene Rhox5 were decreased at the end of the culture period, suggesting that testosterone production by Leydig cells and/or AR transcriptional activity was impaired in organotypic cultures ( 8 ). (elifesciences.org)
MicroRNA-30c expression was suppressed by hypoxia-inducible factor-1α activation and related to decreased mTOR activity as well as glycolysis in human GC TAMs. (cancerindex.org)
In addition, we also revealed that GtrR has moderate GTPase activity in vitro in the absence of its activation signal. (ntu.edu.sg)

In the canonical signaling pathway , binding of a ligand to a cell surface receptor such as a member of the Toll-like receptor superfamily leads to the recruitment of adaptors (such as TRAF) to the cytoplasmic domain of the receptor (Figure 1). (abcam.com)
These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway. (joplink.net)
Activation of the IL-4/STAT6 Pathway promotes lunch cancer progression by increasing M2 myeloid cells. (biocare.net)
By combinatorial screening using H4 and molecules with defined targets, we demonstrated that H4 induces differentiation by the activation of protein kinase C (PKC) signaling pathway, and in line with this, activates PKC phosphorylation and translocation of PKC to the cell membrane. (lu.se)

Brand TM, Iida M, Luthar N, Wleklinski MJ, Starr MM, Wheeler DL (2013) Mapping C-Terminal Transactivation Domains of the Nuclear HER Family Receptor Tyrosine Kinase HER3. (plos.org)
Upon Wnt signaling, ß-catenin binds and activates nuclear TCFs by providing a trans-activation domain. (hubrecht.eu)
A component of promyelocytic leukemia protein nuclear bodies, Daxx has been implicated in apoptosis and characterized as a transcriptional corepressor. (elsevierpure.com)
The responding samples to H4, displayed increased expression of myeloid markers, a clear decrease in the nuclear-cytoplasmic ratio and the potential of re-activation of the monocytic transcriptional program reducing leukemia propagation in vivo. (lu.se)

However, the regions of each receptor that confer transcriptional potential remain poorly defined. (plos.org)

5, 6] The DBD is the most highly conserved domain throughout the steroid receptor family. (medscape.com)

H2A.Z is preferentially crosslinked in vivo to intergenic DNA at the PH05 and GAL1 loci, and this association changes with transcriptional activation. (sdbonline.org)

Mdm2 binds directly to the transcriptional activation domain of p53 and blocks p53-dependent transcription. (molvis.org)

The high-mobility group (HMG) domain is a DNA-binding motif that is shared abundant non-histone components of chromatin and by specific regulators of transcription and cell differentiation. (embl.de)
Large-scale chromatin unfolding and remodeling induced by VP16 acidic activation domain. (ad-astra.ro)
Histone acetylation is important in chromatin remodelling and gene activation. (embl-heidelberg.de)

It utilizes a nuclease-deactivated S. pyogenes Cas9 (dCas9), that is fused to transcriptional activation domains (VP64, p65 and Rta). (horizondiscovery.com)
Enrichment for gene activation can now easily be done using the dCas9-VPR mRNA reagents that co-express either EGFP or puromycin with dCas9-VPR. (horizondiscovery.com)
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)

While the classical membrane-bound functions of HER family RTKs have been extensively studied, accumulating data suggest that these receptors can be found in the cell's nucleus where they can function as co-transcriptional activators [4] , [5] . (plos.org)

Results from these analyses demonstrated that the C-terminal domain of HER3 (CTD, amino acids distal to the tyrosine kinase domain) contained potent transactivation potential. (plos.org)
The NTD of the hGRα contains a major transactivation domain, termed activation function (AF)-1, which is located between amino acids 77 and 262 of the hGRα and is ligand-independent. (medscape.com)

Here, we develop HT-recruit, a pooled assay where protein libraries are recruited to a reporter, and their transcriptional effects are measured by sequencing. (stanford.edu)

High mobility group (HMG) box domains are involved in binding DNA, and may be involved in protein-protein interactions as well. (embl.de)

however, it remains unclear which contain transcriptional effectors. (stanford.edu)

Brm and Rb interact in vitro and in vivo , requiring the Rb-pocket domain and the consensus Rb-binding motif of Brm. (bdbiosciences.com)

This study reports the function of CmNAC1, a stress-responsive pumpkin NAC domain protein. (frontiersin.org)

Subsequently, we used the Gcd− selection to identify domains/residues in eIF2 and tRNAi, eIF1, eIF1A, eIF3, and residues of 18S rRNA located near the 'P' decoding site of the 40S subunit, that participate in rapid TC recruitment in vivo (Figure 1A). (nih.gov)

To accomplish this goal, various intracellular regions of HER3 were fused to the DNA binding domain of the yeast transcription factor Gal4 (Gal4DBD) and tested for their ability to transactivate Gal4 UAS-luciferase. (plos.org)

Finally, we describe how domain movements are associated with the displacement of bound lipids and significant membrane deformations, highlighting the potential regulatory role of the bilayer. (elifesciences.org)

Furthermore, the combinatorial screening identified a bromo- and extra-terminal domain (BET) inhibitor that could further improve H4-dependent leukemic differentiation in FLT3 wild-type monocytic AML. (lu.se)

Pol II pausing and release occur at a point when 5′ end RNA processing and phosphorylation of the Pol II carboxy-terminal domain occurs. (nature.com)
This process leads to the activation of each receptors' tyrosine kinase and the subsequent phosphorylation of tyrosine residues located on their C-terminal tails. (plos.org)
This activation correlates with the differential phosphorylation of Mga at its PTS regulatory domains (PRDs). (umd.edu)

Effect of protein synthesis inhibitors on growth factor activation of c- fos , c- myc , and actin gene transcription. (microbiologyresearch.org)

This family of RTKs has been highly implicated in the formation and progression of various cancers via aberrant overexpression, kinase activation, and mutation [1] , [2] . (plos.org)
The common regulatory step in both of these cascades is activation of an IκB kinase ( IKK ) complex consisting of catalytic kinase subunits (IKKα and/or IKKβ) and the regulatory non-enzymatic scaffold protein NEMO (NF-κB essential modulator also known as IKKγ). (abcam.com)

HOXA7 plays a critical role in metastasis of liver cancer associated with activation of Snail. (nih.gov)

There is even a conserved structure, the bromodomain, that recognizes acetylated residues and may serve as a signalling domain. (embl-heidelberg.de)

Pin1 inhibition improves the efficacy of ralaniten compounds that bind to the N-terminal domain of androgen receptor. (bcgsc.ca)

Transcriptional activities of AR-Vs and full-length AR reside in its N-terminal domain (NTD). (bcgsc.ca)
Here we investigate whether blocking the transcriptional activities of full-length AR and AR-Vs with ralaniten leads to enhanced sensitivity to radiotherapy. (bcgsc.ca)
As a bacterial enhancer-binding protein (bEBP), GtrR is composed of an N-terminal receiver domain, a central ATPases associated with diverse cellular activities (AAA+) ATPase σ54 -interaction domain, and a C-terminal helix-turn-helix DNA-binding domain. (ntu.edu.sg)

Following an initial cleavage by alpha-secretase, the resulting transmembrane domain of Notch is cleaved by gamma-secretase to release the intracellular transcription factor. (nih.gov)
A transcription factor that possesses DNA-binding and E2F-binding domains but lacks a transcriptional activation domain. (bvsalud.org)

Pentapeptide insertion mutagenesis of the Hoxa1 protein: mapping of transcription activation and DNA-binding regulatory domains. (nih.gov)

NF-κB activation leads to expression of the IκBα gene, which functions as a negative feedback loop to sequester NF-κB subunits, terminating signaling unless a persistent activation signal is present. (abcam.com)

Most cells have intracellular sensors of viral za virus and appear partially responsible for the ability of products that, when activated, initiate a signaling cascade influenza viruses to infect multiple animal species, which likely contributes to the generation of new pandemic virus- that results in transcriptional induction of the IFN gene. (cdc.gov)

These transcription factors share homology through a 300 amino acid N-terminal DNA binding/dimerization domain, called the Rel homology domain (RHD). (abcam.com)

Ligand binding to a receptor leads to the recruitment and activation of an IKK complex comprising IKK alpha and/or IKK beta catalytic subunits and two molecules of NEMO. (abcam.com)

Anatomy17

Organisms8

Diseases1

Chemicals and Drugs124

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes78

Information Science4

Activator14

Regulation13

Genes11

Repressor1

Complexes4

Ligand-bindin2

Repression1

Proteins13

Coactivator1

Abiotic stress1

Endogenous3

Glucocorticoid2

Promoter5

Regulator3

TADs2

Exerts1

Activity5

Pathway4

Nuclear4

Confer1

Highly conserved1

Loci1

Binds1

Chromatin3

DCas93

Nucleus1

Transactivation2

Assay1

Interactions1

Unclear1

Motif1

Function1

Vivo1

Yeast1

Describe1

Inhibitor1

Phosphorylation3

Gene transcription1

Kinase2

Role1

Residues1

Improves1

Activities3

Transcription factor2

Regulatory1

Functions1

Results1

Amino1

Catalytic1