• tumor
  • We have used transmission electron microscopy (TEM) to analyze the specificity and the extent of DNA bending upon binding of full-length wild-type human tumor suppressor protein p53 (p53) and the p53 core domain (p53CD) to linear dsDNA bearing the consensus sequence (p53CON). (jbsdonline.com)
  • histone
  • More recently, a number of groups reported the isolation and characterization of a complex termed NuRD (nucleosome remodeling and deacetylating, also NURD and NRD), that not only contains a DNA-dependent ATPase subunit but also histone deacetylase (HDAC) 1/2 ( 11 - 13 ). (pnas.org)
  • Proteins HMG1 and HMG2 are two of the most abundant non histone proteins in the nucleus of mammalian cells, and contain a domain of homology with many proteins implicated in the control of development, such as the sex-determination factor Sry and the Sox family of proteins. (biomedcentral.com)
  • Proteins HMG1 and HMG2, two of the most abundant non histone proteins, have been known for more than 25 years (for a review see [ 1 ]), and their function has been the subject of varied investigations, especially since it was found that they contain a domain of homology with many proteins implicated in the control of development or of differentiation. (biomedcentral.com)
  • transcriptional
  • Expression of BRAF35 containing a single point mutation in the HMG domain of the protein abrogated REST-mediated transcriptional repression. (pnas.org)
  • This complex was shown to act as a transcriptional corepressor for a number of DNA-binding repressors including Mad, the nuclear hormone receptors, and the RE1-binding silencer protein, REST ( 16 , 19 - 22 ). (pnas.org)
  • nuclear
  • Confocal microscopy, using antibodies against the recombinant protein, confirmed its nuclear localization. (pubmedcentralcanada.ca)
  • Nuclear and mitochondrial DNA are thought to be of separate evolutionary origin, with the mtDNA being derived from the circular genomes of the bacteria that were engulfed by the early ancestors of today's eukaryotic cells. (wikipedia.org)
  • In the cells of extant organisms, the vast majority of the proteins present in the mitochondria (numbering approximately 1500 different types in mammals) are coded for by nuclear DNA, but the genes for some, if not most, of them are thought to have originally been of bacterial origin, having since been transferred to the eukaryotic nucleus during evolution. (wikipedia.org)
  • mammalian
  • Intracellular TLR9 was originally described as a pattern recognition receptor (PRR) specific for unmethylated CpG motifs common to bacterial but not mammalian DNA [ 17 ]. (pubmedcentralcanada.ca)
  • However, our studies in B cells, in addition to reports in macrophages, myeloid and plasmacytoid denditic cells (pDCs) have also implicated TLR9 in the detection of endogenous mammalian DNA [ 18 - 20 ]. (pubmedcentralcanada.ca)
  • Mammalian DNA contains relatively few CpG motifs and a remarkably low frequency of CpG dinucleotides, and is highly methylated. (pubmedcentralcanada.ca)
  • Structure
  • R., and Lyubchenko, Y. L. (1998) Structure and dynamics of supercoil-stabilized DNA cruciform. (springer.com)
  • Cruciform DNA is a form of non-B DNA that requires at least a 6 nucleotide sequence of inverted repeats to form a structure consisting of a stem, branch point and loop in the shape of a cruciform, stabilized by negative DNA supercoiling. (wikipedia.org)
  • Several recent studies have suggested that HU binding affinity is enhanced for nicked, gapped or cruciform DNA, indicating that its binding affinity may be regulated by DNA structure (2, 3). (jbsdonline.com)
  • Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. (wikipedia.org)
  • UniProt
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • highly conserved
  • The protein is highly conserved in all metazoans, plants, and yeasts and in the parasites Trypanosoma cruzi ( 49 ), Schistosoma mansoni ( 29 ), and Plasmodium falciparum ( 13 ). (pubmedcentralcanada.ca)
  • mtDNA is highly conserved, and its relatively slow mutation rates (compared to other DNA regions such as microsatellites) make it useful for studying the evolutionary relationships-phylogeny-of organisms. (wikipedia.org)
  • subunit
  • The first of such multiprotein complexes, the SWI/SNF complex, was discovered initially through genetic studies in yeast, and its catalytic subunit, SWI2/SNF2, was identified as the DNA-dependent ATPase ( 2 - 4 ). (pnas.org)
  • Chromosome
  • The Y-Chromosome has definable segments of DNA with known genetic characteristics. (encognitive.com)
  • Although there are several types of markers used in DNA studies, the Y-Chromosome test uses only one type. (encognitive.com)
  • interactions
  • The pathway maps illustrate protein interactions and regulation to provide a comprehensive picture of signaling and disease processes. (bio-rad.com)
  • Bustamante, C. and Rivetti, C. (1996) Visualizing protein-nucleic acid interactions on a large scale with scanning force microscope. (springer.com)
  • Previous fluorescence intensity and anisotropy results from our lab have shown that binding of HU to linear DNA is accompanied by a decrease in stacking interactions indicative of local unwinding and bending of the DNA, supporting the proposal that HU is an architectural protein. (jbsdonline.com)
  • DNA bending due to specific p53 and p53 core domain-DNA interactions visualized by electron microscopy. (jbsdonline.com)
  • structural
  • BRAF35, a structural DNA-binding protein, initially was identified as a component of a large BRCA2-containing complex. (pnas.org)
  • These results show that in the absence of HU, the end-to-end distance in the A-tract duplex is shorter than that predicted from the structural parameters of B-form DNA, indicative of sequence-induced bending. (jbsdonline.com)
  • eukaryotic cells
  • Mitochondrial DNA (mtDNA or mDNA) is the DNA located in mitochondria, cellular organelles within eukaryotic cells that convert chemical energy from food into a form that cells can use, adenosine triphosphate (ATP). (wikipedia.org)
  • nucleus
  • Deoxyribonucleic acid (DNA) is the chemical inside the nucleus of all cells that carries the genetic instructions for making living organisms. (encognitive.com)
  • most of the DNA can be found in the cell nucleus and, in plants and algae, also in plastids such as chloroplasts. (wikipedia.org)
  • interaction
  • Both proteins interacted highly specifically and efficiently with the recognition sequence in the presence of 50 mM KCl at low temperature (~4 ºC) while the p53CD also exhibited a strong and specific interaction at physiological temperature. (jbsdonline.com)
  • The interaction of p53 and the p53CD with p53CON induced a noticeable salt-dependent bending of the DNA axis. (jbsdonline.com)
  • Interaction of full length p53 with the scDNA bearing the p53CON occurred highly specifically at low protein/DNA ratios. (jbsdonline.com)
  • strand
  • The most common repeats include the dinucleotide repeats, which have the bases AC on one DNA strand, and GT on the complementary strand. (wikipedia.org)
  • affinity
  • In this study the goal is to assess the affinity of HU for A-tract DNA, which has been shown to exhibit bending, and to determine the degree of bending induced upon HU binding. (jbsdonline.com)