• recognition
  • The DNA to be cut can be a circular DNA such as in a plasmid, such that it possesses at least one recognition and cutting site outside of the genes or regulatory regions critical to the desired, post-integration function of the DNA, and no recognition and cutting sites within the critical regions. (epo.org)
  • gene
  • The present invention further relates to proteins or other gene products encoded for by the exogenously introduced DNA isolated from said transgenic animals or their descendants. (epo.org)
  • Terminal restriction fragment length polymorphism (TRFLP or sometimes T-RFLP) is a molecular biology technique for profiling of microbial communities based on the position of a restriction site closest to a labelled end of an amplified gene. (wikipedia.org)
  • The method was first described by Liu and colleagues in 1997 which employed the amplification of the 16S rDNA target gene from the DNA of several isolated bacteria as well as environmental samples. (wikipedia.org)
  • together
  • The DNA and the corresponding restriction enzyme can be introduced into the sperm cells, together or sequentially, by electroporation or lipofection or by any other technique that preserves enough of said sperm's mobility and fertilization functions. (epo.org)
  • addition
  • In addition to the recombinant DNA field, the analytical hybridization technique can be applied to the detection of polynucleotides of importance in the fields of human and veterinary medicine, agriculture, and food science, among others. (google.ca)
  • In addition to these steps the TRFLP protocol often includes a cleanup of the PCR products prior to the restriction and in case a capillary electrophoresis is used a desalting stage is also performed prior to running the sample. (wikipedia.org)
  • double
  • WO9942569A1 ] The present invention relates to high efficiency methods and compositions for stably integrating exogenous double-stranded DNA into the chromosomal DNA of sperm cells. (epo.org)