• recombinant
  • The principle of nucleic acid hybridization assays was developed by workers in the recombinant DNA field as a means for determining and isolating particular polynucleotide base sequences of interest. (google.ca)
  • In addition to the recombinant DNA field, the analytical hybridization technique can be applied to the detection of polynucleotides of importance in the fields of human and veterinary medicine, agriculture, and food science, among others. (google.ca)
  • Prize in Medicine (1978) for the discovery of restriction endonucleases, leading to the development of recombinant DNA technology. (powershow.com)
  • probe
  • A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or RNA.RNA hybrids with the particular polynucleotide sequence to be determined. (google.ca)
  • 8. The method of claim 1 wherein the particular polynucleotide to be determined is RNA or DNA and said probe is substantially composed of RNA. (google.ca)
  • double
  • WO9942569A1 ] The present invention relates to high efficiency methods and compositions for stably integrating exogenous double-stranded DNA into the chromosomal DNA of sperm cells. (epo.org)
  • together
  • The DNA and the corresponding restriction enzyme can be introduced into the sperm cells, together or sequentially, by electroporation or lipofection or by any other technique that preserves enough of said sperm's mobility and fertilization functions. (epo.org)
  • sites
  • The DNA to be cut can be a circular DNA such as in a plasmid, such that it possesses at least one recognition and cutting site outside of the genes or regulatory regions critical to the desired, post-integration function of the DNA, and no recognition and cutting sites within the critical regions. (epo.org)