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  • ATPase
  • The present study showed that Arg 694 of the Orc1p subunit is important for the ATPase activity of ORC and suggests that this ATPase activity is required for efficient MCM loading on to origin DNA and for progression of S phase. (biochemj.org)
  • fork
  • Furthermore, we demonstrated a dynamic association of Hat1p with chromatin during S-phase that suggests a role of this enzyme at the replication fork. (biomedcentral.com)
  • The two most logical times for the loss of such determinants are at the replication fork, where chromatin is reassembled, or during mitosis when nuclear architecture is dismantled. (rupress.org)
  • cells
  • This system has allowed us to evaluate the preparedness of cells for executing a replication program. (rupress.org)
  • Moreover, ORC derived from orc2ts cells is defective for DNA binding. (sciencemag.org)
  • Cells employ multiple mechanisms to repair or tolerate DNA lesions in order to maintain genomic integrity. (pubmedcentralcanada.ca)
  • Many clinically relevant chemotherapeutic agents, including mitomycin C, cisplatin, and nitrogen mustard, target tumor cells by virtue of their ability to covalently cross-link complementary DNA strands, introducing ICLs into the genome. (pubmedcentralcanada.ca)
  • processes
  • Human DNA polymerase kappa (pol κ) is a translesion synthesis (TLS) polymerase that catalyzes TLS past various minor groove lesions including N 2 -dG linked acrolein- and polycyclic aromatic hydrocarbon-derived adducts, as well as N 2 -dG DNA-DNA interstrand cross-links introduced by the chemotherapeutic agent mitomycin C. It also processes ultraviolet light-induced DNA lesions. (pubmedcentralcanada.ca)
  • However
  • However, to date, no specific DNA sequence elements have been identified that predict the locations of origins in a multicellular eukaryote ( Gilbert, 2001a ). (rupress.org)
  • However, other activities (ATP binding and origin DNA binding) were indistinguishable from those of wild-type ORC. (biochemj.org)
  • proper
  • Additional genetic and biochemical evidence points to the existence of partly overlapping histone H3 acetyltransferase activities in addition to Hat1p/Hat2p for proper DNA replication efficiency. (biomedcentral.com)
  • specific
  • Cdc6p increases the DNA binding specificity of ORC by inhibiting non-specific DNA binding of ORC. (cshl.edu)
  • shown
  • In addition to its role in the bypass of N 2 -dG lesions, pol κ has also been shown to play a role in the processing of various ultraviolet (UV) light-induced DNA lesions - . (pubmedcentralcanada.ca)
  • helicase
  • At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. (genome.jp)
  • Studies on murine Mcm2 indicate that its C terminus is required for interaction with MCM4, as well as for inhibition of the DNA helicase activity of the Mcm4,6,7 complex. (ebi.ac.uk)
  • fission yeast
  • In Mitchison's lab he made substantial contributions to the study of the cell cycle in fission yeast isolating and characterising cell cycle mutants and the first identification of a gene product (DNA ligase) in these mutants. (wikipedia.org)
  • genes
  • Both the ORB sequence and the adjacent initiator gene are considerably diverse among different replication origins, while in silico and genetic analyses have indicated the specificity between the initiator genes and their cognate origins. (frontiersin.org)
  • DNA is wrapped around histones, and, by transferring an acetyl group to the histones, genes can be turned on and off. (wikipedia.org)
  • nucleotide
  • In E. coli, the direction for orisome assembly are built into a short stretch of nucleotide sequence called as origin of replication (oriC) which contains multiple binding sites for the initiator protein DnaA (a highly homologous protein amongst bacterial kingdom). (wikipedia.org)
  • sequences
  • DNA sequences also form complex 3-dimensional structures, such as telomeres, centromeres and ribosomal DNA. (reference.com)
  • In the last two decades of the 20th century, botanists exploited the techniques of molecular genetic analysis, including genomics and proteomics and DNA sequences to classify plants more accurately. (wikipedia.org)
  • transcription
  • The molecule's structure is key to its function and replication, and it controls its own replication and transcription. (brighthub.com)
  • When it was first discovered, it was thought that acetylation of lysine neutralizes the positive charge normally present, thus reducing affinity between histone and (negatively charged) DNA, which renders DNA more accessible to transcription factors. (wikipedia.org)
  • The protein encoded by this gene is a zinc finger DNA binding transcription factor that interacts with CIP1 (p21 / CDKN1A), part of a complex with cyclin E. The encoded protein may regulate the cellular localization of CIP1. (wikipedia.org)
  • replicates
  • DNA makes proteins, but it also replicates itself by splitting the double helix down the middle to create two templates which then form the basis of a new double helix, and the process continues. (brighthub.com)
  • Okazaki
  • FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA. (genome.jp)
  • Molecular Biology
  • This book collects the Proceedings of a workshop sponsored by the European Molecular Biology Organization (EMBO) entitled "Pro- teins Involved in DNA Replication" which was held September 19 to 23,1983 at Vitznau, near Lucerne, in Switzerland. (springer.com)
  • Cdt1
  • DNA replication factor Cdt1 is a protein that in humans is encoded by the CDT1 gene. (wikipedia.org)
  • Its activity during the cell cycle is tightly regulated by its association with the protein geminin, which both inhibits Cdt1 activity during S phase in order to prevent re-replication of DNA and prevents it from ubiquitination and subsequent proteolysis. (wikipedia.org)
  • CDT1 belongs to a family of replication proteins conserved from yeast to humans. (wikipedia.org)
  • Examples of orthologs in other species include: S. pombe - cdt1 (cdc10-dependent transcript 1) Drosophila melanogaster - 'double parked' or Dup Xenopus laevis - Cdt1 DNA replication factor CDT1 has been shown to interact with SKP2. (wikipedia.org)
  • sequence
  • There are 11 DnaA binding sites/boxes on the E. coli origin of replication out of which three boxes R1, R2 and R4 (which have a highly conserved 9 bp consensus sequence 5' - TTATC/ACACA ) are high affinity DnaA boxes. (wikipedia.org)
  • Sequence Search Submit a protein or DNA sequence for SCOP superfamily and family level classification using the SUPERFAMILY HMM's. (wikipedia.org)
  • ATPase
  • The present study showed that Arg 694 of the Orc1p subunit is important for the ATPase activity of ORC and suggests that this ATPase activity is required for efficient MCM loading on to origin DNA and for progression of S phase. (biochemj.org)
  • fork
  • Furthermore, we demonstrated a dynamic association of Hat1p with chromatin during S-phase that suggests a role of this enzyme at the replication fork. (biomedcentral.com)
  • The two most logical times for the loss of such determinants are at the replication fork, where chromatin is reassembled, or during mitosis when nuclear architecture is dismantled. (rupress.org)
  • cells
  • This system has allowed us to evaluate the preparedness of cells for executing a replication program. (rupress.org)
  • Moreover, ORC derived from orc2ts cells is defective for DNA binding. (sciencemag.org)
  • Cells employ multiple mechanisms to repair or tolerate DNA lesions in order to maintain genomic integrity. (pubmedcentralcanada.ca)
  • Many clinically relevant chemotherapeutic agents, including mitomycin C, cisplatin, and nitrogen mustard, target tumor cells by virtue of their ability to covalently cross-link complementary DNA strands, introducing ICLs into the genome. (pubmedcentralcanada.ca)
  • processes
  • Human DNA polymerase kappa (pol κ) is a translesion synthesis (TLS) polymerase that catalyzes TLS past various minor groove lesions including N 2 -dG linked acrolein- and polycyclic aromatic hydrocarbon-derived adducts, as well as N 2 -dG DNA-DNA interstrand cross-links introduced by the chemotherapeutic agent mitomycin C. It also processes ultraviolet light-induced DNA lesions. (pubmedcentralcanada.ca)
  • However
  • However, to date, no specific DNA sequence elements have been identified that predict the locations of origins in a multicellular eukaryote ( Gilbert, 2001a ). (rupress.org)
  • However, other activities (ATP binding and origin DNA binding) were indistinguishable from those of wild-type ORC. (biochemj.org)
  • proper
  • Additional genetic and biochemical evidence points to the existence of partly overlapping histone H3 acetyltransferase activities in addition to Hat1p/Hat2p for proper DNA replication efficiency. (biomedcentral.com)
  • specific
  • Cdc6p increases the DNA binding specificity of ORC by inhibiting non-specific DNA binding of ORC. (cshl.edu)
  • shown
  • In addition to its role in the bypass of N 2 -dG lesions, pol κ has also been shown to play a role in the processing of various ultraviolet (UV) light-induced DNA lesions - . (pubmedcentralcanada.ca)