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  • recombinant
  • The principle of nucleic acid hybridization assays was developed by workers in the recombinant DNA field as a means for determining and isolating particular polynucleotide base sequences of interest. (google.ca)
  • In addition to the recombinant DNA field, the analytical hybridization technique can be applied to the detection of polynucleotides of importance in the fields of human and veterinary medicine, agriculture, and food science, among others. (google.ca)
  • Prize in Medicine (1978) for the discovery of restriction endonucleases, leading to the development of recombinant DNA technology. (powershow.com)
  • single-stranded
  • Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. (google.ca)
  • DNA and RNA, such as obtained by denaturing their double stranded forms, will hybridize or recombine under appropriate conditions with complementary single stranded nucleic acids. (google.ca)
  • By labeling such complementary probe nucleic acids with some readily detectable chemical group, it was then made possible to detect the presence of any polynucleotide sequence of interest in a test medium containing sample nucleic acids in single stranded form. (google.ca)
  • assay
  • proposed the name M. chimaera for strains that a reverse hybridization-based line probe assay suggested belonged to MAIS (M. avium-M. intracellulare-M. scrofulaceum group) but were different from M. avium, M. intracellulare, or M. scrofulaceum. (wikipedia.org)
  • The direct DNA hybridization approach is the simplest suspension array assay whereby 15 to 20 bp DNA oligonucleotides attached to microspheres are amplified using PCR. (wikipedia.org)
  • Fluorescent
  • Here, dynamic DNA complexes detect a specific DNA-conjugated antibody and undergo strand displacement to liberate a quencher strand and activate a fluorescent reporter. (rice.edu)
  • FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, whereas CISH probes are labelled with biotin or digoxigenin and can be detected using a bright-field microscope after other treatment steps have been applied. (wikipedia.org)
  • Each type of microsphere bead contains a known DNA probe with a unique fluorescent identity. (wikipedia.org)
  • fragments
  • DNA probes can also be used to identify the presence of complementary sequences in a mix of fragments. (thefreedictionary.com)
  • DNA is extracted from cells used to create test fragments. (wikipedia.org)
  • Test fragments and probes on the microsphere beads are allowed to hybridize to each other. (wikipedia.org)
  • Micronuclei form during anaphase from lagging acentric chromosome or chromatid fragments caused by incorrectly repaired or unrepaired DNA breaks or by nondisjunction of chromosomes. (wikipedia.org)
  • One way is that disrepair of DNA double-strand breaks can lead to symmetrical or asymmetrical chromatid and chromosome exchanges as well as chromatid and chromosome fragments. (wikipedia.org)
  • If DNA damage exceeds the repair capacity of the cell, unrepaired double-stranded DNA breaks may also result in acentric chromosome fragments. (wikipedia.org)
  • Another way eccentric chromosome fragments may arise is when defects in genes related to homologous recombinational repair (ex: ATM, BRCA1, BRCA2, and RAD51) result in a dysfunctional error-free homologous recombinational DNA repair pathway and causes the cell to resort to the error-prone non-homologous end-joining (NHEJ) repair pathway, increasing the likelihood of incorrect repair of DNA breaks, formation of dicentric chromosomes, and acentric chromosome fragments. (wikipedia.org)
  • fluorescence
  • Given the ability to visualize large numbers of cellular markers using dynamic DNA probe complexes, high-content proteomic analyses can be performed on a single sample, enhancing the power of fluorescence imaging techniques. (rice.edu)
  • Attached to one end of the probe is a fluorophore and to the other end a fluorescence quencher. (wikipedia.org)
  • antibodies
  • We also applied our DNA probes to immunofluorescence imaging using DNA-conjugated antibodies and demonstrated the ability to at least double the number of detectable markers on a single sample. (rice.edu)
  • specificity
  • Single copy DNA probes offer a specificity in hybridizing genetic chromosomes not heretofore available for identifying elusive strains of inherited genetic diseases and cancers, allowing, among other things, for more precise clinical treatment. (technologynetworks.com)
  • genes
  • This incorrect segregation of chromosomes may result from hypomethylation of repeat sequences present in pericentromeric DNA, irregularities in kinetochore proteins or their assembly, dysfunctional spindle apparatus, or flawed anaphase checkpoint genes. (wikipedia.org)
  • Diagnostic Market
  • DNA Probe-based Diagnostic Market report helps to increase business/sales activities by understanding competitor's businesses better, company's strategic, business and operational performance, recognize potential partnerships and suppliers. (sbwire.com)
  • New York, NY -- ( SBWIRE ) -- 04/16/2018 -- The latest market intelligence study on DNA Probe-based Diagnostic market relies on statistics derived from the application of both primary and secondary research to present insights pertaining to the operational model, opportunities and competitive landscape of DNA Probe-based Diagnostic market for the forecast period, 2018 - 2025. (sbwire.com)
  • In addition, the research on the DNA Probe-based Diagnostic market concentrates on extracting valuable data on swelling investment pockets, significant growth opportunities and major market vendors to help understand business owners what their competitors are doing best to stay ahead in the competition. (sbwire.com)
  • The research also segments the DNA Probe-based Diagnostic market on the basis of the end-user, product type, application and demography for the forecast period 2018 - 2025. (sbwire.com)
  • For more clarity on the real potential of the DNA Probe-based Diagnostic market for the forecast period of 2018 - 2025 the study provides vital intelligence on the major opportunities, threats and challenges posed by the industry. (sbwire.com)
  • What are major driving factors impacting the DNA Probe-based Diagnostic market worldwide? (sbwire.com)
  • Which market trends from the yesteryears and the future are likely to keep the prospect of the DNA Probe-based Diagnostic market high for the forecast period 2018 - 2025? (sbwire.com)
  • Chlamydia
  • Chlamydia species can exchange DNA between the different strains, thus the evolution of new strains is common. (wikipedia.org)
  • Chlamydia species are readily identified and distinguished from other Chlamydia species using DNA-based tests. (wikipedia.org)
  • amplification
  • The Company plans to combine the probes with its proprietary signal amplification technologies, which will enhance their effectiveness by allowing visualization at levels not achievable by other methods. (technologynetworks.com)
  • Captured target enrichment If the probe is linearized, traditional PCR amplification is performed to enrich the captured target using the universal primers of the probe. (wikipedia.org)
  • Otherwise, rolling circle amplification is performed for the circular probe. (wikipedia.org)
  • strands
  • Enzo Biochem, Inc. has announced that, as a result of a licensing agreement, it plans to enter into the cytogenetics market by producing newly patented single copy DNA probes that can identify the minutest strands of DNA, opening the door to effective treatment of genetic diseases and some cancers. (technologynetworks.com)
  • restriction
  • The internal region contains two universal PCR primer sites that are common to all MIPs as well as a probe-release site, which is usually a restriction site. (wikipedia.org)
  • Probe release In some versions of the protocol, the probe-release site (commonly a restriction site) is cleaved by restriction enzymes such that the probe becomes linearized. (wikipedia.org)
  • The tags from each probe are released by cleaving the tag release site with restriction enzymes. (wikipedia.org)
  • hybridizes
  • With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. (wikipedia.org)
  • genetic
  • Enzo also acquired rights to approximately 50 DNA probes developed at the University, which in peer review publications have been listed as being able to identify a number of genetic maladies. (technologynetworks.com)
  • The PGD allows studying the DNA of eggs or embryos to select those that carry certain mutations for genetic diseases. (wikipedia.org)
  • proteins
  • I didn't gel purify my PCR product, I simply removed the proteins using a Millilpore spin prep tube and precipitated the DNA. (bio.net)
  • When using different types of microspheres, SAT is capable of simultaneously testing multiple variables, such as DNA and proteins, in a given sample. (wikipedia.org)
  • specific
  • If biotin was used as a probe label, non-specific binding sites must first be blocked using bovine serum albumin (BSA). (wikipedia.org)
  • After washing to remove non-specific binding between sequences, only strongly paired probe-target will remain hybridized. (wikipedia.org)
  • The challenge of this approach is reducing cross-hybridization between the allele-specific probes. (wikipedia.org)
  • Dynamic allele-specific hybridization (DASH) genotyping takes advantage of the differences in the melting temperature in DNA that results from the instability of mismatched base pairs. (wikipedia.org)
  • chromosomal
  • The license agreement grants Ikonisys a worldwide license under Abbott patents for the manufacture and sale of DNA probes in conjunction with Chromotest(TM), a revolutionary test for the prenatal diagnosis of chromosomal abnormalities using fetal cells from the maternal circulation. (thefreedictionary.com)
  • Hypomethylation of cytosine in centromeric and pericentromeric areas and higher-order repeats of satellite DNA in centromeric DNA can result in such chromosomal loss events. (wikipedia.org)
  • instability
  • Classical satellite DNA is normally heavily methylated at cytosine residues but may become almost fully unmethylated due to ICF syndrome (Immunodeficiency, centromere instability, and facial anomalies syndrome) or after treatment by DNA methyl transferase inhibitors. (wikipedia.org)
  • target
  • Specifically, the two target complementary regions at the 5' and 3' ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. (wikipedia.org)
  • These probes, however, are designed such that a gap delimited by the hybridized ends of the probes remains over the target region. (wikipedia.org)
  • The captured target can also be identified by sequencing the probe, now also containing the target. (wikipedia.org)
  • Probe-target hybridization is usually detected by optically labeled targets, which determines the relative abundance of each target in the sample. (wikipedia.org)
  • Similarly, target DNA samples are usually PCR amplified and labeled. (wikipedia.org)
  • different
  • My probe is a 350 bp PCR product from an insert that I identified using a different vector system. (bio.net)
  • These probes allow us to now drill down to the DNA level and see that the cause of the disease may actually be slightly different between patients with the same diagnosis. (technologynetworks.com)
  • After amplifying one DNA oligoprobe of interest, it can be used to create 100 different probes on 100 different sets of microspheres, each with the capability of capturing 100 potential targets (if using a 100-plex array). (wikipedia.org)
  • recombinant
  • The principle of nucleic acid hybridization assays was developed by workers in the recombinant DNA field as a means for determining and isolating particular polynucleotide base sequences of interest. (google.ca)
  • In addition to the recombinant DNA field, the analytical hybridization technique can be applied to the detection of polynucleotides of importance in the fields of human and veterinary medicine, agriculture, and food science, among others. (google.ca)
  • Prize in Medicine (1978) for the discovery of restriction endonucleases, leading to the development of recombinant DNA technology. (powershow.com)
  • single-stranded
  • Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. (google.ca)
  • DNA and RNA, such as obtained by denaturing their double stranded forms, will hybridize or recombine under appropriate conditions with complementary single stranded nucleic acids. (google.ca)
  • By labeling such complementary probe nucleic acids with some readily detectable chemical group, it was then made possible to detect the presence of any polynucleotide sequence of interest in a test medium containing sample nucleic acids in single stranded form. (google.ca)
  • nucleic
  • Rieper, Anders and Vedral modelled the electron clouds of nucleic acids in a single strand of DNA as a chain of coupled quantum harmonic oscillators with dipole-dipole interaction between nearest neighbours. (xonl.de)
  • cells
  • Cell routine evaluation After removal of methanol, cells had been treated having a Coulter DNA-Prep reagent package (Beckman-Coulter, France). (cancercurehere.com)
  • primer pair
  • Multiplex ligation-dependent probe amplification (MLPA) is a variation of the multiplex polymerase chain reaction that permits amplification of multiple targets with only a single primer pair. (wikipedia.org)
  • genetic
  • However, comprehensive genetic testing (such as through the use of DNA arrays or full genome sequencing) allows for the estimation of disease risk years to decades before any disease even exists, or even whether a healthy fetus is at higher risk for developing a disease in adolescence or adulthood. (wikipedia.org)
  • Additionally, new tests from Genetic Technologies LTD and Phenogen Sciences Inc. comparing non-coding DNA to a woman's lifetime exposure to estrogen can now determine a woman's probability of developing estrogen positive breast cancer also known as sporadic breast cancer (the most prevalent form of breast cancer). (wikipedia.org)
  • FISH is often used for finding specific features in DNA for use in genetic counseling, medicine, and species identification. (wikipedia.org)
  • strand
  • Asymmetric PCR is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other. (wikipedia.org)
  • gene
  • A direct mechanism of action involves homodimerization of the receptor, translocation via active transport into the nucleus, and binding to specific DNA responsive elements activating gene transcription. (wikipedia.org)
  • molecular
  • He developed concepts for a workbench to manipulate individual macromolecules and supramolecular systems, employing scanning probe microscopies, light, and molecularly modified graphite surfaces, which has been used to correlate structure and dynamics of molecular systems with mechanical, electronic, optical, and (bio)chemical properties from molecular to macromolecular lengths and time scales. (wikipedia.org)
  • results
  • After his arrest, he agreed to provide blood and saliva samples, and the DNA results connected him to the murder of Janene Weinhold. (wikipedia.org)
  • sample
  • If an extra copy is present in the test sample, the signals are expected to be 1.5 times the intensities of the respective probes from the reference. (wikipedia.org)
  • If a and b are the signals from two amplicons in the patient sample, and A and B are the corresponding amplicons in the experimental control, then the dosage quotient DQ = (a/b) / (A/B). Although dosage quotients may be calculated for any pair of amplicons, it is usually the case that one of the pair is an internal reference probe. (wikipedia.org)
  • Repetitive DNA sequences must be blocked by adding short fragments of DNA to the sample. (wikipedia.org)
  • specific
  • It was developed by biomedical researchers in the early 1980s and is used to detect and localize the presence or absence of specific DNA sequences on chromosomes. (wikipedia.org)
  • Use of Asymmetric PCR to Generate Long Primers and Single-stranded DNA for Incorporating Cross-linking Analogs into Specific Sites in a DNA Probe" (PDF). (wikipedia.org)
  • example
  • For example, the stability of DNA with respect to the UV irradiation is due to such conical intersection. (wikipedia.org)
  • allows
  • The new method, published in the Journal of Clinical Microbiology , allows scientists to enrich Mtb DNA directly from patient sputum (mucus) samples. (medicalnewstoday.com)
  • single
  • Asymmetric PCR can be used to form single stranded DNA from double stranded DNA, which is then used for DNA sequencing in the mutagenesis method. (wikipedia.org)
  • citation needed] Single stranded DNA is also important for aptamer generation. (wikipedia.org)
  • complete
  • Each complete probe has a unique length, so that its resulting amplicons can be separated and identified by (capillary) electrophoresis. (wikipedia.org)
  • important
  • The location and characterization of conical intersections are therefore essential to the understanding of a wide range of important phenomena governed by non-adiabatic events, such as photoisomerization, photosynthesis, vision and the photostability of DNA. (wikipedia.org)
  • Positive
  • Positive territory sequencing should radically be confused with dna profiling, which n't determines the roof that military dairy came from a added school or election, and does primarily contain mobile surgeon on next opponents, violence or design to appropriate contents. (tenant-credit-check.life)