• complementary
  • DNA and RNA, such as obtained by denaturing their double stranded forms, will hybridize or recombine under appropriate conditions with complementary single stranded nucleic acids. (google.ca)
  • By labeling such complementary probe nucleic acids with some readily detectable chemical group, it was then made possible to detect the presence of any polynucleotide sequence of interest in a test medium containing sample nucleic acids in single stranded form. (google.ca)
  • Complementary portions of labeled probe and mRNA from the pool to be analyzed form hybrids that are resistant to digestion with single strandspecific RNase T1 and RNase A. Because of its sensitivity, the RPA can, in general, be performed on total RNA preparations derived by standard methods from either frozen tissues or cultured cells, without further purification of polyA+ RNA. (bio-medicine.org)
  • hybridization
  • A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or RNA.RNA hybrids with the particular polynucleotide sequence to be determined. (google.ca)
  • The principle of nucleic acid hybridization assays was developed by workers in the recombinant DNA field as a means for determining and isolating particular polynucleotide base sequences of interest. (google.ca)
  • In addition to the recombinant DNA field, the analytical hybridization technique can be applied to the detection of polynucleotides of importance in the fields of human and veterinary medicine, agriculture, and food science, among others. (google.ca)
  • These enzymes possess several properties that make them well suited for the preparation of highly specific hybridization probes. (bio-medicine.org)
  • The RPA technique is based on the hybridization in solution of a labeled anti-sense probe RNA with the RNA pool being tested. (bio-medicine.org)
  • assay
  • The assay was made possible by the discovery and characterization of bacteriophageencoded DNA-dependent RNA polymerases (SP6, T7, and T3). (bio-medicine.org)
  • To generate a probe for use in this assay, it is necessary to subclone a fragment that contains the cDNA sequences of interest downstream of one of the mentioned phage promoters. (bio-medicine.org)
  • We suggest the use of a relatively small probe for this assay (100300 nucleotides). (bio-medicine.org)
  • RNase contamination: When probe fragmentation is observed in the protection assay, check the integrity of your RNA sample by agarose electrophoresis. (bio-medicine.org)
  • presence
  • Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. (google.ca)
  • Subsequent detection reveals the presence of target mRNA in t he sample by the appearance of an appropriately sized probe fragment. (bio-medicine.org)