• cleavage
  • In agreement with the above results, a comparison of the REP consensus sequence with the sequence of previously identified pBR322 "strong" gyrase cleavage sites reveals a high degree of homology. (nih.gov)
  • We have analysed the DNA cleavage reaction of DNA gyrase using oligonucleotides annealed to a singlestranded M13 derivative containing a preferred gyrase cleavage site. (le.ac.uk)
  • With either drug, cleavage will not occur within 6 bases from the end of a DNA duplex or a nick. (le.ac.uk)
  • We suggest that cleavage site specificity with short DNA duplexes is determined by drug-DNA interactions whereas with longer fragments the positioning effect of the DNA wrap around gyrase prescribes the site of cleavage. (le.ac.uk)
  • CL-promoted DNA cleavage mediated by gyrase occurs at specific sites and is stimulated by ATP. (nih.gov)
  • These drug concentrations were chosen so as to achieve comparable cleavage levels of the linear DNA substrate. (nih.gov)
  • The specificity of gyrase-mediated DNA breakage promoted by CL was examined using the 5′-end 33P-labelled S fragment from the S. pneumoniae parE gene and compared to that of gemifloxacin, an anti-pneumococcal quinolone whose induction of sequence-specific cleavage has been studied in detail for pneumococcal gyrase and topo IV (31). (nih.gov)
  • Cleavage of TOP and BOTTOM DNA strands by both drugs was markedly enhanced by the inclusion of ATP and required the presence of gyrase: no breakage was seen with CL alone consistent with the known poor reactivity of CL toward duplex DNA (Figure 3, Introduction section). (nih.gov)
  • vitro
  • Following on from the finding that the complex of quinolone and gyrase on DNA results in truncated mRNAs in vitro, the results presented in this thesis show that in vivo, the addition of quinolone drugs results in the production of truncated mRNAs which are presumably translated into truncated proteins. (bl.uk)
  • Gene
  • Foury F, Lahaye A. Cloning and sequencing of the PIF gene involved in repair and recombination of yeast mitochondrial DNA. (pubmedcentralcanada.ca)
  • The 290-bp S fragment (from the S. pneumoniae parE gene) 33P-labelled at the 5′ end of the TOP or BOTTOM strand was cleaved by S. pneumoniae gyrase in the absence or presence of 200 μM CL or 100 μM gemifloxacin (Gemi) and 1 mM ATP. (nih.gov)
  • binds
  • DNA gyrase binds to the family of prokaryotic repetitive extragenic palindromic sequences. (nih.gov)
  • Regulation of the ccd operon by the CCdA/CCdB complex is dependent upon the ratio of the two molecules to each other in the complex: a (CcdA)2-(CcdB)2 complex binds the DNA of the operon thus repressing transcription, but when CcdB is in excess of CcdA de-repression occurs, whereas repression will occur when CcdA levels are greater than or equal to that of CcdB. (wikipedia.org)
  • Molecule
  • A single molecule study has characterized gyrase activity as a function of DNA tension (applied force) and ATP, and proposed a mechanochemical model. (wikipedia.org)
  • affinity
  • Gyrase has at least a 10-fold higher affinity for DNA containing REP sequences than for DNA not containing REP sequences. (nih.gov)
  • mutations
  • In conclusion, the presence of mutations at amino acids 50 and 119 suggests that in addition to QRDR section and Tyr-122, these sites are also essential for DNA gyrase activity. (ac.ir)
  • reaction
  • Therefore, it can be suggested that two ATP molecules are hydrolyzed per cycle of reaction by gyrase, leading to the introduction of a linking difference of -2. (wikipedia.org)
  • chromosomal
  • This process appears similar to the degradation of chromosomal DNA that occurs during apoptosis of some eukaryotic cells. (bl.uk)
  • occurs
  • This process occurs in prokaryotes (in particular, in bacteria), whose single circular DNA is cut by DNA gyrase and the two ends are then twisted around each other to form supercoils. (wikipedia.org)
  • interaction
  • Because REP sequences are numerous and found throughout the genome, we suggest they have physiological functions mediated through their interaction with gyrase, such as being sites of action for the maintenance of DNA supercoiling. (nih.gov)
  • cell
  • The majority of DNA is located in the cell nucleus, where it is called nuclear DNA. (reference.com)
  • The University of Illinois at Chicago explains that DNA must replicate itself so that during cell division, both daughter cells receive the same genetic in. (reference.com)
  • This short video describes our new Cell Based Screening Systems for DNA Repair. (topogen.com)