• plasmid
  • A physical map of nonkiller plasmid DNA revealed the presence of two circular 63-kb du-plexes, each with a single BamH I site and a region of homology of about 17 kb in length. (springer.com)
  • We report here that the killer plasmid DNA consists of four circular 63-kb duplexes, two of which are indistinguishable with respect to their restriction maps and possess two BamH I sites. (springer.com)
  • We show also that under axenic conditions of growth, plasmid from both killer and nonkiller xenosomes is amplified during late stationary growth phase of the protozoa. (springer.com)
  • As pan-kDNA most closely resembles a DNA plasmid, it may be the ancestral form of kDNA. (wikipedia.org)
  • Ribosomal
  • Phylogenetic analyses of small subunit ribosomal DNA (SSU rDNA) sequence data from Atlantic cod X-cells show that they are a related parasite that occupies a basal position to the clade containing other X-cell parasites. (nih.gov)
  • parasites
  • Pooled Chelex extraction of DNA, followed by nested PCR of cytochrome b, was the optimal strategy, allowing reliable detection of a single low-parasitemic sample (100 parasites/μl) in pool sizes up to 50. (nih.gov)
  • Epidermal pseudotumours from Hippoglossoides dubius and Acanthogobius flavimanus in Japan and gill lesions in Limanda limanda from the UK have been shown to be caused by phylogenetically related protozoan parasites, known collectively as X-cells. (nih.gov)
  • The protozoa are intracellular parasites that infect the erythrocytes. (wikipedia.org)
  • He processed the tissue sample of the enlarged spleen using a staining technique (now known as Leishman's stain) which he had just developed, and discovered the protozoan parasites using microscopy. (wikipedia.org)
  • But he mistakenly considered the parasites to be degenerate trypanosomes, already known protozoan parasites in Africa and South America. (wikipedia.org)
  • parasite
  • We first used laboratory-prepared samples to compare 2 DNA extraction and 4 PCR detection methods across a range of pool sizes and parasite densities. (nih.gov)
  • For instance, many DNA sequences have been identified for Haemoproteus in birds around the world in recent years, leading to new knowledge about the previously unknown diversity of this parasite in different regions The infective stage is the sporozoite which is present in the salivary glands of the vector. (wikipedia.org)
  • trypanosomes
  • The aims were to evaluate loop-mediated isothermal amplification (LAMP) for detection of trypanosomal DNA in clinical samples and to characterize the infecting trypanosomes to the subspecies level. (curehunter.com)
  • After his mandatory retirement in 1999, Borst became a staff member of the NKI-AVL and continued running his lab, studying mechanisms of chemotherapy resistance in cancer cells, the physiological functions of drug transporters and the biosynthesis and function of DNA Base J, a new base in DNA, discovered in trypanosomes in the Borst lab. (wikipedia.org)
  • A DNA transposition mechanism for antigenic variation in African trypanosomes (9) (10) (11) (12). (wikipedia.org)
  • In trypanosomes, a group of flagellated protozoans, the kinetoplast exists as a dense granule of DNA within the large mitochondrion. (wikipedia.org)
  • polymerase chain re
  • This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications. (atcc.org)
  • homologous
  • Conjugation requires stable and extended contact between a donor and a recipient strain, is DNase resistant, and the transferred DNA is incorporated into the recipient chromosome by homologous recombination . (wikipedia.org)
  • 1986
  • Soldo, A.T., and A.F. Freytag (1986) Symbiont xenosome DNA is a chimera with respect to Dam methylation of GATC sequences. (springer.com)
  • Soldo, A.T., S.A. Brickson, G.A. Castiglione, and A.F. Freytag (1986) Association of transformation of xenosomes from non-killer to killer with extrachromosomal DNA. (springer.com)
  • genome
  • They are defined as sections of DNA removed from the diploid micronuclear genome during which a copy of the micronuclear genome is converted to the macronuclear genome even though errors occur in which an IES sequence may not be deleted. (wikipedia.org)
  • sequences
  • Southern, E. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. (springer.com)
  • Detection
  • Comparative detection of trypanosomal DNA by loop-mediated isothermal amplification and PCR from flinders technology associates cards spotted with patient blood. (curehunter.com)
  • stain
  • Its kinetoplast is easily visible in samples stained with DAPI, a fluorescent DNA stain, or by the use of fluorescent in situ hybridization (FISH) with BrdU, a thymidine analogue. (wikipedia.org)
  • circular
  • Recently, we identified extrachromosomal DNA in the form of covalently closed circular duplexes, each 63 kb in length, from both killer and nonkiller xenosomes. (springer.com)
  • The kinetoplast contains circular DNA in two forms, maxicircles and minicircles. (wikipedia.org)
  • samples
  • 6,000/μl, highlighting the issue of possible DNA degradation with long-term storage of samples. (nih.gov)
  • another
  • H. volcani forms cytoplasmic bridges between cells that appear to be used for transfer of DNA from one cell to another in either direction. (wikipedia.org)
  • group
  • However, the phylogenetic position of the X-cell group is not well supported within any of the existing protozoan phyla and they are currently thought to be members of the Alveolata.Ultrastructural features of X-cells in fish pseudotumours are somewhat limited and no typical environmental stages, such as spores or flagellated cells, have been observed. (nih.gov)
  • cell
  • Transduction is the process by which foreign DNA is introduced into a cell by a virus or viral vector . (wikipedia.org)
  • members
  • Laveran and his colleague Félix Mesnil identified the protozoan (and yet wrongly) to be members of Piroplasmida, and gave the scientific name Piroplasma donovanii. (wikipedia.org)
  • African
  • We analyzed DNA eluted from FTA (Flinders Technology Associates) cards spotted with blood from human African trypanosomiasis (HAT) patients admitted at Lwala Hospital in eastern Uganda and Kaliua Health Centre in northwestern Tanzania. (curehunter.com)