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  • genetics
  • She has conducted post-doctoral research on bacteriophage genetics at Stanford University, bacterial DNA replication at the Boston Biomedical Research Institute and studied axenic cultivation at the National Institutes of Health. (wikipedia.org)
  • Discovered a new arm of the Milky Way galaxy Una M. Ryan, (1966) patented DNA test identifying the protozoan parasite Cryptosporidium Helen Alma Newton Turner (1908-1995), geneticist and statistician, expert on sheep genetics Carden Wallace (fl. (wikipedia.org)
  • plasmid
  • Biodistribution of DNA plasmid vaccines against HIV-1, Ebola, Severe Acute Respiratory Syndrome, or West Nile virus is similar, without integration, despite differing plasmid backbones or gene inserts. (semanticscholar.org)
  • A physical map of nonkiller plasmid DNA revealed the presence of two circular 63-kb du-plexes, each with a single BamH I site and a region of homology of about 17 kb in length. (springer.com)
  • We report here that the killer plasmid DNA consists of four circular 63-kb duplexes, two of which are indistinguishable with respect to their restriction maps and possess two BamH I sites. (springer.com)
  • We show also that under axenic conditions of growth, plasmid from both killer and nonkiller xenosomes is amplified during late stationary growth phase of the protozoa. (springer.com)
  • As pan-kDNA most closely resembles a DNA plasmid, it may be the ancestral form of kDNA. (wikipedia.org)
  • Genes
  • The uptake of donor DNA and its recombinational incorporation into the recipient chromosome depends on the expression of numerous bacterial genes whose products direct this process. (wikipedia.org)
  • She is recognized primarily for her in-depth study of Entamoeba histolytica, a parasitic protozoan that causes amoebiasis: Dr. Bhattacharya's laboratory first detected Ribosomal RNA genes on Circular DNA, while studying the parasite, and also discovered families of retrotransposons in the parasite genome. (wikipedia.org)
  • Parasites
  • Pooled Chelex extraction of DNA, followed by nested PCR of cytochrome b, was the optimal strategy, allowing reliable detection of a single low-parasitemic sample (100 parasites/μl) in pool sizes up to 50. (nih.gov)
  • Epidermal pseudotumours from Hippoglossoides dubius and Acanthogobius flavimanus in Japan and gill lesions in Limanda limanda from the UK have been shown to be caused by phylogenetically related protozoan parasites, known collectively as X-cells. (nih.gov)
  • Phylogenetic analyses of small subunit ribosomal DNA (SSU rDNA) sequence data from Atlantic cod X-cells show that they are a related parasite that occupies a basal position to the clade containing other X-cell parasites. (nih.gov)
  • The protozoa are intracellular parasites that infect the erythrocytes. (wikipedia.org)
  • He processed the tissue sample of the enlarged spleen using a staining technique (now known as Leishman's stain) which he had just developed, and discovered the protozoan parasites using microscopy. (wikipedia.org)
  • But he mistakenly considered the parasites to be degenerate trypanosomes, already known protozoan parasites in Africa and South America. (wikipedia.org)
  • parasite
  • We first used laboratory-prepared samples to compare 2 DNA extraction and 4 PCR detection methods across a range of pool sizes and parasite densities. (nih.gov)
  • We present a strategy for whole-genome amplification (WGA) of low-yield samples from P. falciparum prior to short-read sequencing.We show that this method reduces amplification bias and chimera formation.Our data show that this method is suitable for as low as 10 pg input DNA, and offers the possibility of sequencing the parasite genome from small blood samples. (nih.gov)
  • For instance, many DNA sequences have been identified for Haemoproteus in birds around the world in recent years, leading to new knowledge about the previously unknown diversity of this parasite in different regions The infective stage is the sporozoite which is present in the salivary glands of the vector. (wikipedia.org)
  • genome
  • They are defined as sections of DNA removed from the diploid micronuclear genome during which a copy of the micronuclear genome is converted to the macronuclear genome even though errors occur in which an IES sequence may not be deleted. (wikipedia.org)
  • Genomic DNA
  • Different amounts of P. falciparum 3D7 genomic DNA (ranging from 2 ng to 100 fg as shown in sample labels) were used as an input template for amplification by Repli_g following the standard or optimized procedure. (nih.gov)
  • trypanosomes
  • The aims were to evaluate loop-mediated isothermal amplification (LAMP) for detection of trypanosomal DNA in clinical samples and to characterize the infecting trypanosomes to the subspecies level. (curehunter.com)
  • After his mandatory retirement in 1999, Borst became a staff member of the NKI-AVL and continued running his lab, studying mechanisms of chemotherapy resistance in cancer cells, the physiological functions of drug transporters and the biosynthesis and function of DNA Base J, a new base in DNA, discovered in trypanosomes in the Borst lab. (wikipedia.org)
  • A DNA transposition mechanism for antigenic variation in African trypanosomes (9) (10) (11) (12). (wikipedia.org)
  • In trypanosomes, a group of flagellated protozoans, the kinetoplast exists as a dense granule of DNA within the large mitochondrion. (wikipedia.org)
  • homologous
  • Conjugation requires stable and extended contact between a donor and a recipient strain, is DNase resistant, and the transferred DNA is incorporated into the recipient chromosome by homologous recombination . (wikipedia.org)
  • ATCC
  • The ATCC Protozoa DNA Panels comprise nucleic acid preparations isolated from taxonomic and medically-relevant strains. (atcc.org)
  • nuclear
  • As a ciliated protozoan, Tetrahymena thermophila exhibits nuclear dimorphism: two types of cell nuclei. (wikipedia.org)
  • The replication of the kinetoplast occurs simultaneously to the duplication of the adjacent flagellum and just prior to the nuclear DNA replication. (wikipedia.org)
  • samples
  • 6,000/μl, highlighting the issue of possible DNA degradation with long-term storage of samples. (nih.gov)
  • This report describes the evaluation of multiple procedures for DNA extraction and qPCR-based protocols aiming to establish a standardized methodology for the absolute quantification of T. cruzi DNA in Guanidine - EDTA blood (GEB) samples. (curehunter.com)
  • Standard curve parameters such as, amplification efficiency, coefficient of determination and intercept were evaluated, as well as different procedures to generate standard samples containing pre-established T. cruzi DNA concentration. (curehunter.com)
  • Samples amplified using the optimized procedure showed a decrease in chimer formation that remained low and steady from 2 ng to 100 pg of input DNA. (nih.gov)
  • Its kinetoplast is easily visible in samples stained with DAPI, a fluorescent DNA stain, or by the use of fluorescent in situ hybridization (FISH) with BrdU, a thymidine analogue. (wikipedia.org)
  • 1986
  • Soldo, A.T., S.A. Brickson, G.A. Castiglione, and A.F. Freytag (1986) Association of transformation of xenosomes from non-killer to killer with extrachromosomal DNA. (springer.com)
  • Detection
  • Comparative detection of trypanosomal DNA by loop-mediated isothermal amplification and PCR from flinders technology associates cards spotted with patient blood. (curehunter.com)
  • vaccines
  • Over the past 15 years, DNA vaccines have gone from a scientific curiosity to one of the most dynamic research field and may offer new alternatives for the control of parasitic diseases such as leishmaniasis and Chagas disease. (semanticscholar.org)
  • We review here some of the advances and challenges for the development of DNA vaccines against these diseases. (semanticscholar.org)
  • DNA vaccines: recent developments and future possibilities. (semanticscholar.org)
  • extraction
  • The DNA extraction product was then used as a template for cytochrome b nested PCR. (nih.gov)
  • The results achieved from Bayesian transmutability analysis elected the primer sets Cruzi1/Cruzi2 (p=0.0031) and Diaz7/Diaz8 (p=0.0023) coupled to the QIAamp DNA Kit extraction protocol ( silica gel column), as the most suitable for monitoring parasitemia in these patients. (curehunter.com)
  • circular
  • Recently, we identified extrachromosomal DNA in the form of covalently closed circular duplexes, each 63 kb in length, from both killer and nonkiller xenosomes. (springer.com)
  • The kinetoplast contains circular DNA in two forms, maxicircles and minicircles. (wikipedia.org)
  • presence
  • The DNA was extracted, and analysed with PCR and sequencing for the presence of arthropod-borne apicomplexan protozoa. (biomedcentral.com)
  • On the other hand, to the best of our knowledge, this method was hitherto not used to reveal the presence of arthropod-borne protozoa bats may have contact with. (biomedcentral.com)
  • major
  • Comparison of potential protection induced by three vaccination strategies (DNA/DNA, Protein/Protein and DNA/Protein) against Leishmania major infection using Signal Peptidase type I in BALB/c mice. (semanticscholar.org)
  • a protozoan of major medical and economic importance. (nih.gov)
  • bacteria
  • The synthetic amino-psoralen, amotosalen HCl, has been developed for the inactivation of infectious pathogens (bacteria, viruses, protozoa) in platelet and plasma blood components prepared for transfusion support of patients. (wikipedia.org)
  • Plasmodium
  • PMID 8944026), profiling gene expression throughout the lifecycle of the malaria-causing protozoan Plasmodium falciparum, his discovery of the SARS virus, and pioneering virus discovery using gene hybridization array and DNA sequencing technologies (Wang et al. (wikipedia.org)
  • nucleic acids
  • Ultraviolet germicidal irradiation (UVGI) is a disinfection method that uses short-wavelength ultraviolet (UV-C) light to kill or inactivate microorganisms by destroying nucleic acids and disrupting their DNA, leaving them unable to perform vital cellular functions. (wikipedia.org)
  • molecules
  • In 1989, while returning from a scientific meeting in Oregon, Deamer conceived that it might be possible to sequence single molecules of DNA by using an imposed voltage to pull them individually through a nanoscopic channel. (wikipedia.org)
  • MU81-MMS4, in the budding yeast Saccharomyces cerevisiae, is a DNA structure-selective endonuclease that cleaves joint DNA molecules formed during homologous recombination in meiosis and mitosis. (wikipedia.org)
  • molecular
  • Particularly at wavelengths around 260 nm-270 nm, UV breaks molecular bonds within microorganismal DNA, producing thymine dimers that can kill or disable the organisms. (wikipedia.org)
  • Search
  • In 1957, Deamer submitted his research on self-organizing protozoa to the Westinghouse Science Talent Search and was among the 40 winners who were invited to Washington DC that year. (wikipedia.org)
  • cell
  • We driven the development profile of fourteen cancers cell lines by plating the cells on the soft-plate96 and calculating the flip transformation in cell amount after five times using a neon DNA-binding absorb dyes (Fig. 2). (techtasys.com)
  • Telomerase replaces short bits of DNA known as telomeres, which are otherwise shortened when a cell divides via mitosis. (wikipedia.org)
  • Telomerase allows each offspring to replace the lost bit of DNA allowing the cell line to divide without ever reaching the limit. (wikipedia.org)
  • This takes into consideration a phenomenon known as light and dark repair (photoreactivation and base excision repair, respectively), in which a cell can repair DNA that has been damaged by UV light. (wikipedia.org)
  • class
  • In some systems of classification, ciliated protozoa are placed within the class "Ciliata," (a term which can also refer to an unrelated genus of fish). (wikipedia.org)
  • studies
  • As a result of modern investigations (ultrastructural and biochemical studies and, more recently, phylogenetic analyses based on DNA sequencing), we now know that the differences among these three groups run far deeper than the color differences suggested by their names. (eol.org)