• genetics
  • She has conducted post-doctoral research on bacteriophage genetics at Stanford University, bacterial DNA replication at the Boston Biomedical Research Institute and studied axenic cultivation at the National Institutes of Health. (wikipedia.org)
  • Discovered a new arm of the Milky Way galaxy Una M. Ryan, (1966) patented DNA test identifying the protozoan parasite Cryptosporidium Helen Alma Newton Turner (1908-1995), geneticist and statistician, expert on sheep genetics Carden Wallace (fl. (wikipedia.org)
  • Ribosomal
  • Phylogenetic analyses of small subunit ribosomal DNA (SSU rDNA) sequence data from Atlantic cod X-cells show that they are a related parasite that occupies a basal position to the clade containing other X-cell parasites. (nih.gov)
  • The primer set amplified a specific region of Leishmania internal transcribed spacers of the ribosomal DNA locus. (nih.gov)
  • She is recognized primarily for her in-depth study of Entamoeba histolytica, a parasitic protozoan that causes amoebiasis: Dr. Bhattacharya's laboratory first detected Ribosomal RNA genes on Circular DNA, while studying the parasite, and also discovered families of retrotransposons in the parasite genome. (wikipedia.org)
  • Genes
  • The uptake of donor DNA and its recombinational incorporation into the recipient chromosome depends on the expression of numerous bacterial genes whose products direct this process. (wikipedia.org)
  • genome
  • They are defined as sections of DNA removed from the diploid micronuclear genome during which a copy of the micronuclear genome is converted to the macronuclear genome even though errors occur in which an IES sequence may not be deleted. (wikipedia.org)
  • parasites
  • Pooled Chelex extraction of DNA, followed by nested PCR of cytochrome b, was the optimal strategy, allowing reliable detection of a single low-parasitemic sample (100 parasites/μl) in pool sizes up to 50. (nih.gov)
  • Epidermal pseudotumours from Hippoglossoides dubius and Acanthogobius flavimanus in Japan and gill lesions in Limanda limanda from the UK have been shown to be caused by phylogenetically related protozoan parasites, known collectively as X-cells. (nih.gov)
  • The protozoa are intracellular parasites that infect the erythrocytes. (wikipedia.org)
  • He processed the tissue sample of the enlarged spleen using a staining technique (now known as Leishman's stain) which he had just developed, and discovered the protozoan parasites using microscopy. (wikipedia.org)
  • But he mistakenly considered the parasites to be degenerate trypanosomes, already known protozoan parasites in Africa and South America. (wikipedia.org)
  • plasmid
  • A physical map of nonkiller plasmid DNA revealed the presence of two circular 63-kb du-plexes, each with a single BamH I site and a region of homology of about 17 kb in length. (springer.com)
  • We report here that the killer plasmid DNA consists of four circular 63-kb duplexes, two of which are indistinguishable with respect to their restriction maps and possess two BamH I sites. (springer.com)
  • We show also that under axenic conditions of growth, plasmid from both killer and nonkiller xenosomes is amplified during late stationary growth phase of the protozoa. (springer.com)
  • Mammalian
  • It is also present in another group of arthropods belonging to the class Collembola, a protozoan group Ciliophora, mammalian trophoblast and antipodal and suspensor cells in plants. (wikipedia.org)
  • sequences
  • Soldo, A.T., and A.F. Freytag (1986) Symbiont xenosome DNA is a chimera with respect to Dam methylation of GATC sequences. (springer.com)
  • Southern, E. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. (springer.com)
  • For instance, many DNA sequences have been identified for Haemoproteus in birds around the world in recent years, leading to new knowledge about the previously unknown diversity of this parasite in different regions The infective stage is the sporozoite which is present in the salivary glands of the vector. (wikipedia.org)
  • homologous
  • Conjugation requires stable and extended contact between a donor and a recipient strain, is DNase resistant, and the transferred DNA is incorporated into the recipient chromosome by homologous recombination . (wikipedia.org)
  • trypanosomes
  • After his mandatory retirement in 1999, Borst became a staff member of the NKI-AVL and continued running his lab, studying mechanisms of chemotherapy resistance in cancer cells, the physiological functions of drug transporters and the biosynthesis and function of DNA Base J, a new base in DNA, discovered in trypanosomes in the Borst lab. (wikipedia.org)
  • A DNA transposition mechanism for antigenic variation in African trypanosomes (9) (10) (11) (12). (wikipedia.org)
  • 1986
  • Soldo, A.T., S.A. Brickson, G.A. Castiglione, and A.F. Freytag (1986) Association of transformation of xenosomes from non-killer to killer with extrachromosomal DNA. (springer.com)
  • Replication
  • Specialized cells undergo repeated rounds of DNA replication without cell division (endomitosis), to increase cell volume, forming a giant polytene chromosome. (wikipedia.org)
  • Their primary functions are: to act as binding sites for RNA pol II, to initiate replication and, to start nucleosome remodeling of short fragments of DNA. (wikipedia.org)
  • Detection
  • We first used laboratory-prepared samples to compare 2 DNA extraction and 4 PCR detection methods across a range of pool sizes and parasite densities. (nih.gov)
  • group
  • However, the phylogenetic position of the X-cell group is not well supported within any of the existing protozoan phyla and they are currently thought to be members of the Alveolata.Ultrastructural features of X-cells in fish pseudotumours are somewhat limited and no typical environmental stages, such as spores or flagellated cells, have been observed. (nih.gov)
  • cell
  • Transduction is the process by which foreign DNA is introduced into a cell by a virus or viral vector . (wikipedia.org)
  • H. volcani forms cytoplasmic bridges between cells that appear to be used for transfer of DNA from one cell to another in either direction. (wikipedia.org)
  • members
  • Laveran and his colleague Félix Mesnil identified the protozoan (and yet wrongly) to be members of Piroplasmida, and gave the scientific name Piroplasma donovanii. (wikipedia.org)
  • samples
  • 6,000/μl, highlighting the issue of possible DNA degradation with long-term storage of samples. (nih.gov)