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  • strand
  • RuvA and RuvB bind to the four strand DNA structure formed in the Holliday junction intermediate, and migrate the strands through each other, using a putative spooling mechanism. (wikipedia.org)
  • The poly G tail found at either end of the DNA strand can vary in length and even number (Type D only have a poly G sequence on the 3'end), but its presence is critical to the activity of the molecule. (wikipedia.org)
  • This primer serves to prime leading strand DNA synthesis. (wikipedia.org)
  • Gram
  • I wonder which protocol should I follow, since I am not working with bacterial suspensions proper, and in the other hand I do not want to miss gram positives due to lysis failure. (protocol-online.org)
  • single-stranded
  • In these high salt concentrations, the eukaryotic histone protein is eluted from a DNA solution in which single stranded DNA is bound covalently to cellulose. (wikipedia.org)
  • CpG oligodeoxynucleotides (or CpG ODN) are short single-stranded synthetic DNA molecules that contain a cytosine triphosphate deoxynucleotide ("C") followed by a guanine triphosphate deoxynucleotide ("G"). The "p" refers to the phosphodiester link between consecutive nucleotides, although some ODN have a modified phosphorothioate (PS) backbone instead. (wikipedia.org)
  • strands
  • The binding of the RuvC protein to the RuvAB complex is thought to cleave the DNA strands, thereby resolving the Holliday junction. (wikipedia.org)
  • transcription
  • This domain is the binding site for the antibacterial drug rifampin (and its analogues) which blocks the DNA/RNA tunnel and prevents initiation of transcription. (ebi.ac.uk)
  • H-NS is about 15.6 kDa and assists in the regulation of bacterial transcription in bacteria by repressing and activating certain genes. (wikipedia.org)
  • molecules
  • As DNA molecules are much less absorptive than water molecules in the THz range, the RCA products on the surface of the MBs cause a significant decrease in THz absorption, which can be sensitively probed by THz spectroscopy. (rsc.org)
  • acute
  • Fleroxacin is effective in the treatment of a wide variety of infections, particularly uncomplicated cystitis in women, acute uncomplicated pyelonephritis, gonorrhea, bacterial enteritis, traveler's diarrhea, respiratory tract infections ( including exacerbation of chronic bronchitis). (wikipedia.org)
  • residues
  • There is an N-terminal Zinc-binding domain (residues 1-110) where a zinc ion is tetrahedrally coordinated between one histidine and three cysteine residues, which plays a role in recognizing sequence specific DNA binding sites. (wikipedia.org)
  • synthetic
  • demonstrated that the CpG motif within bacterial DNA was responsible for the immunostimulatory effects and developed synthetic CpG ODN. (wikipedia.org)
  • Synthetic CpG ODN differ from microbial DNA in that they have a partially or completely phosphorothioated (PS) backbone instead of the typical phosphodiester backbone and a poly G tail at the 3' end, 5' end, or both. (wikipedia.org)
  • junctions
  • With an α-helical hydrophobic core and two positively charged β-ribbon arms, HU binds non-specifically to dsDNA with low affinity but binds to altered DNA-such as junctions, nicks, gaps, forks, and overhangs-with high affinity. (wikipedia.org)
  • RuvA is a DNA-binding protein that binds Holliday junctions with high affinity. (wikipedia.org)
  • genome
  • Bacterial DNA may integrate into the human genome more readily in tumors than in normal human tissue, scientists have found. (nsf.gov)
  • Scientists have already shown that bacteria can transfer DNA to the genome of an animal. (nsf.gov)
  • They found that bacterial DNA was more likely to integrate in the genome in tumor samples than in normal, healthy somatic cells. (nsf.gov)
  • The 3-D structure of the human genome, shown in a stretch of DNA inside a fractal globule. (nsf.gov)
  • signatures
  • Bacterial DNA signatures in carotid atherosclerosis represent both commensals and pathogens of skin origin. (biomedsearch.com)
  • By using a novel subtraction broad-range PCR approach, we defined bacterial DNA signatures in surgically removed sterile carotid artery endarterectomy plaques of patients with carotid atherosclerosis. (biomedsearch.com)
  • resistance
  • Jiande Gu and Jerzy Leszczynski and colleagues from Jackson State University and the Chinese Academy of Sciences ran a computational study that revealed that the base-stacking structure of DNA could increase the resistance of the arsenate towards hydrolysis, compared with current arsenate models. (rsc.org)
  • The efficacy of nitrofurantoin in treating UTIs combined with a low rate of bacterial resistance to this agent makes it one of the first-line agents for treating uncomplicated UTIs as recommended by the Infectious Diseases Society of America and the European Society for Microbiology and Infectious Diseases. (wikipedia.org)
  • cell
  • According to findings of Professor Zsolt Balohh and his team at University of Newcastle (Australia), mitochondrial DNA is the leading cause of severe inflammation due to a massive amount of Mitochondrial DNA that leaks into the blood stream due to cell death into the blood stream of patients that survived major trauma. (wikipedia.org)
  • Since 1893, it has been recognized that Coley's toxin, a mixture of bacterial cell lysate, has immunostimulatory properties that could reduce the progression of some carcinomas, but it was not until 1983 that Tokunaga et al. (wikipedia.org)
  • scientists
  • According to the researchers, the discovery means scientists need to consider a significant new factor, the DNA of microbes passed from mother to child, in their efforts to understand how genes influence illness and health. (medindia.net)
  • DNA fingerprinting is a technique which helps forensic scientists and legal experts solve crimes, identity thefts, legal suits and terrorism cases. (medindia.net)
  • Now, scientists from the US and China say that arsenic substituted DNA may be more stable than first thought. (rsc.org)
  • His discovery, while working in the laboratory of Paul Doty at Harvard University, that the denaturation of DNA was reversible (DNA hybridization) and depended on salt- and GC-content, had a major impact on how scientists thought about DNA, and how DNA could be handled in vitro. (wikipedia.org)
  • genes
  • Uptake of such genes could provide a mechanism for facilitating recovery from DNA damage after genotoxic stress. (wikipedia.org)
  • affinity
  • Its crystal structure has been solved at 1.9A. RuvB is an ATPase that is only active in the presence of DNA and compared to RuvA, RuvB has a low affinity for DNA. (wikipedia.org)
  • environments
  • With recent demonstrations of nanopore sequencing in Antarctica and onboard the International Space Station, the ability to reliably characterize m6A presents an opportunity to further examine the role of methylation in bacterial adaptation to extreme environments. (nanoporetech.com)
  • specific
  • Mice studies by researchers at Washington University School of Medicine in St. Louis have shown that bacterial DNA can pass from parent to offspring in a manner that affects specific traits such as immunity and inflammation. (medindia.net)
  • The proposed strategy not only represents a new method for the isothermal detection of the target bacterial DNA but also provides a general methodology for sensitive and specific DNA biosensing using THz spectroscopy. (rsc.org)
  • rapid
  • An unstable SSB/DNA system would result in rapid disintegration of the SSB, which stalls DNA replication. (wikipedia.org)
  • The demand for rapid and sensitive bacterial detection is continuously increasing due to the significant requirements of various applications. (rsc.org)
  • found
  • Bacterial DNA is typically found in rings like this, which are known as plasmids. (sciencephoto.com)
  • The researchers found that while only 63.5 percent of TCGA samples analyzed were from tumors, the tumor samples contained 99.9 percent of reads supporting bacterial integration. (nsf.gov)
  • digestion
  • DNA and RNA is ready for qPCR, RT-PCR and next-generation sequencing, while protein is ready for 1D SDS-PAGE and mass spectrometry following in-gel trypsin digestion. (qiagen.com)
  • However
  • However, our study also suggested that arsenated DNA (As-DNA) is still less stable than normal DNA when hydrolysis is considered,' says Leszczynski. (rsc.org)
  • role
  • The phenomenon might play a role in cancer and other diseases associated with DNA damage. (nsf.gov)