• Q4: Is it necessary to add beta-mercaptoethanol? (mbpinc.net)
  • Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). (mbpinc.net)
  • Yes you can use beta-mercaptoethanol as an alternative to DTT if you don't have DTT. (pegaswitch.com)
  • Generally DTT is preferred because it is not volatile and therefore doesn't smell nearly as much as beta-mercaptoethanol, is effective at lower concentrations and is more stable. (pegaswitch.com)
  • Cu2+, Mo2+, Mg2+, Zn2+, phenylmethanesulfonyl fluoride (PMSF), dithiothreitol (DTT) and beta-mercaptoethanol (2-ME) enhanced the enzyme activity, whereas Ca2+ and ethylene-diaminetetraacetic acid (EDTA) had inhibitory effect. (csircentral.net)
  • The column was washed three times with one column volume of buffer A prior to elution by the application of one column volume of buffer B (50 mM Tris [pH 7.6], 500 mM NaCl, 10% glycerol, 0.1% NP-40, 250 mM imidazole, 5 mM -mercaptoethanol). (bioinbrief.com)
  • Disulfide groups can be converted to SH groups by reducing agents such as dithiothreitol (DTT) or β mercaptoethanol. (dojindo.com)
  • The presence of a functional disulfide bond was tested initially with 1 and 10 mm dithiothreitol (data not shown), which experienced little effect on the agonist-independent current. (bioskinrevive.com)
  • 2-Mercaptoethanol is in extensive uses including protection of SH group in a protein, breakage of disulfide bond and extraction of crude proteins. (fujifilm.com)
  • Reducing agents, such as dithiothreitol (DTT) or β-mercaptoethanol, are commonly used to cleave disulfide bonds via thiol-disulfide bond exchange reactions. (bocsci.com)
  • Under reducing conditions (50 mM dithiothreitol, 10 mM 2-mercaptoethanol or 1% sodium borohydride), the disulfide bonds are cleaved, releasing the biotin tag and any avidin conjugate bound to it. (broadpharm.com)
  • For typsin digestion, 10 mM Dithiothreitol (DTT) was added to samples (10 g/sample) for the reduction of disulfide bonds and incubated at 37°C for 30 min. (cdc.gov)
  • The Ligase-65 enzyme remains unchanged, but the β-mercaptoethanol antioxidant in its storage buffer will be replaced by DTT because this is less pungent and less toxic. (mrcholland.com)
  • Protein samples are normally added to sample buffer, containing sds, β-mercaptoethanol or dithiothreitol. (we-consent.org)
  • The new MLPA buffer contains the antioxidant dithiothreitol (DTT). (mrcholland.com)
  • Bacterial suspensions were mixed with Laemmli buffer (5% -mercaptoethanol were added before use) and boiled for 10 min. (cdc.gov)
  • TCEP can keep the cysteines from forming di-sulfide bonds and unlike dithiothreitol and β-mercaptoethanol, it will not react as readily with the maleimide. (pegaswitch.com)
  • A smaller reducing agent, 2-mercaptoethanol (BME), was successful at reducing the glycine-only current by 70% (Fig. 2and illustrate block of EI with glutamate only and KN with glycine only. (bioskinrevive.com)
  • 3-Mercapto-1,2-Propanediol is a new reducing agent replacing 2-mercaptoethanol. (fujifilm.com)
  • TCEP-HCl is an odor- less (non-volatile) reducing agent that has been found to be more stable and effective than dithiothreitol (DTT) and able to work well at lower pH levels. (pegaswitch.com)
  • It has been confirmed to show reducing potential equal to 2-mercaptoethanol by the SDS-PAGE. (fujifilm.com)
  • Moreover, free thiols, as dithiothreitol (DTT) and mercapto ethanol, can deteriorate or completely prevent click reactions. (baseclick.eu)
  • Note The Amplite™ Red is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol. (aatbio.com)
  • After purification by Ni-Sepharose affinity chromatography, the Tα1-Intein fusion protein was induced by the thiols β-mercaptoethanol or d,l-dithiothreitol, or by increasing the temperature, to release Tα1 through intein-mediated N-terminal cleavage. (biomedcentral.com)
  • Bacterial suspensions were mixed with Laemmli buffer (5% -mercaptoethanol were added before use) and boiled for 10 min. (cdc.gov)
  • Ten microliters of AF was diluted 1:2 v/v with Laemmli test buffer filled with 5% v/v β-mercaptoethanol (BME) (Bio-Rad La Jolla CA) and decreased by boiling for 5 min. (cancerrealitycheck.com)
  • 293T cells (5 105) that were transfected with pTag2B-BDLF1 or pTag2B-BORF1 had been lysed with 1 ml lysis buffer that included 1 mM EDTA, 5 mM dithiothreitol, 0.5% NP-40, and 1 mM phenylmethylsulfonyl fluoride in phosphate-buffered saline. (rockstarsagainstliveearth.com)
  • The final concentration of DTT or 2-mercaptoethanol in the reaction should be no higher than 10 µM. (aatbio.com)
  • At 1 mM final concentration, 2-mercaptoethanol, dithiothreitol, ethylenediamine tetraacetic acid and p-methylphenyl sulfonylfluoride inhibited the esterolytic reaction. (ktu.edu.tr)
  • A reducing agent such as mercaptoethanol or dithiothreitol (DTT) (in the presence of a detergent i.e. (myweb.ge)
  • Because GSH uptake measurements in isolated membrane vesicles are normally carried out in the presence of 5-10 mM dithiothreitol (DTT) to maintain the tripeptide in the reduced form, the present study examined the effects of DTT and other sulfhydryl-reducing agents on Ycf1p- and Mrp2-mediated transport activity. (nih.gov)