Four conserved cysteines are involved in disulfide bonds. (wikipedia.org)
The two monomer chains are similar (~250 kDa each) and are linked together by disulFide bonds. (mnsu.edu)
The dimer structure mediated by the carboxy-terminal interchain disulfide bonds is also essential. (princeton.edu)
Fibronectin (Science: protein) glycoprotein of high molecular weight (2 chains each of 250 kd linked by disulphide bonds). (biologyonline.com)
The reactive organosulfur compounds form disulfide bonds with free sulfhydryl groups of enzymes and compromise the integrity of the bacterial membrane. (frontiersin.org)
However, other PTPRJ complexes, dependent on the formation of disulfide bonds, are detected by treatment with the oxidant agent H(2)O(2) or by a mutation Asp872Cys, located in the eighth fibronectin type III domain of PTPRJ. (unina.it)
A deletion in the eighth fibronectin domain of PTPRJ impairs its dimerization in the plasma membrane and increases the formation of PTPRJ complexes dependent on disulfide bonds that remain trapped in the cytoplasm. (unina.it)
Walker S., Ullman O., Stultz CM. Using Intramolecular Disulfide Bonds in Tau Protein to Deduce Structural Features of Aggregation-resistant Conformations. (mit.edu)
Native thrombospondin is a 450,000 molecular weight homotrimer consisting of three identical peptide chains linked through interchain disulfide bonds. (goprolytix.com)
With a total of 177 amino acids, IL-17A forms two pairs of antiparallel β-sheets and maintains its structural stability through multiple disulfide bonds. (betalifesci.com)
The presence of two disulfide bonds results in a highly folded structure. (justia.com)
Chen,V.M. and Hogg,P.J. (2006): Allosteric disulfide bonds in thrombosis and thrombolysis. (9dok.net)
two identical heavy chains and two identical light chains connected by disulfide bonds. (medscape.com)
Extracellular matrix8
Each domain consists of two antiparallel beta sheets and is folded topologically identically to single fibronectin type III domains from the extracellular matrix proteins tenascin and fibronectin. (embl.de)
van der Loo JC, Xiao X, McMillin D, Hashino K, Kato I, Williams DA: VLA-5 is expressed by mouse and human long-term repopulating hematopoietic cells and mediates adhesion to extracellular matrix protein fibronectin. (exbio.cz)
Fibronectin (FN) is a large glycoprotein dimer (500 kDa) that exists in either plasma or the extracellular matrix. (mnsu.edu)
During extracellular matrix assembly, fibronectin (FN) binds to cell surface receptors and initiates fibrillogenesis. (princeton.edu)
Fibronectin (cold-insoluble globulin) is found in plasma and in the extracellular matrix. (haemochrom.de)
These domains are necessary for the function: Fibronectin mediates cell adhesion to components of the extracellular matrix via cellular receptors. (haemochrom.de)
Tenascin is a large, disulfide-bonded glycoprotein of the extracellular matrix. (duke.edu)
A disulfide cyclic peptide ligand [CTVRTSADC] 1 has been previously found to target extra domain B of fibronectin (EDB-FN) in the extracellular matrix that can di erentiate aggressive PCa from benign prostatic hyperplasia. (chapman.edu)
Glycoprotein4
Fibronectin is a dimeric glycoprotein composed of disulfide-linked subunits with a molecular weight of 220-250kDa each. (embl.de)
The fibrillary glycoprotein is composed of two peptide chains that are connected by a disulfide bridge. (haemochrom.de)
Structurally, thrombospondin is a 450,000 molecular weight glycoprotein (1) consisting of three, identical, disulfide-linked polypeptide chains (6-8). (goprolytix.com)
ITGA2b associates with integrin beta 3 to form complexes that interact with fibrinogen, von Willebrand factor, fibronectin, and vitronectin. (rndsystems.com)
Collagen2
Fibronectin is a plasma protein that binds cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. (wikipedia.org)
Thrombospondin interacts with a variety of extracellular macromolecules including heparin (1,17), collagen (18), fibrinogen and fibronectin (19), plasminogen (20), plasminogen activator (21), and osteonectin (22). (goprolytix.com)
Protein4
Fibronectin, type I repeats are one of the three repeats found in the fibronectin protein. (wikipedia.org)
One of three types of internal repeat within the plasma protein, fibronectin. (embl.de)
The eighth fibronectin type III domain of protein tyrosine phosphatase receptor J influences the formation of protein complexes and cell localization. (unina.it)
IL-17R is a transmembrane protein with two fibronectin III-like domains (FN domains) located outside the cell membrane, responsible for mediating ligand-receptor binding. (betalifesci.com)
Fibrinogen1
In the cold, fibronectin is co-precipitated with fibrinogen (cold-insoluble globulin). (haemochrom.de)
Thrombospondin1
1. Matthias LJ, Gotis-Graham I, Underwood PA, McNeil HP, Hogg PJ (1996) Identification of monoclonal antibodies that recognize different disulphide bonded forms of thrombospondin 1. (bioporto.com)
Chains2
It is cleaved into two disulfide-linked chains (114 kDa and 22 kDa) during transit through the Golgi. (rndsystems.com)
CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. (exbio.cz)
Binds1
VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. (exbio.cz)
Repeats4
Fibronectin type-III (FN3) repeats are both the largest and the most common of the fibronectin subdomains. (embl.de)
We have determined the 2.0 A crystal structure of a fragment of human fibronectin encompassing the seventh through the RGD-containing tenth type III repeats (FN7-10). (embl.de)
We report the crystal structure of two adjacent fibronectin type III repeats from the Drosophila neural cell adhesion molecule neuroglian. (embl.de)
The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. (arigobio.com)
Proteins1
Monobodies are the founding system of a set of synthetic binding proteins based on the fibronectin type III (FN3) domain. (nih.gov)
Subunits2
Disulfide-bonded heterodimer of 40 kda and 35 kda subunits. (lu.se)
The molecular weight of the native human hexabrachion is thus consistent with a disulfide-bonded hexamer of the 320 kDa subunits. (duke.edu)
Thrombosis1
Wang Y, Ni H. Fibronectin maintains the balance between hemostasis and thrombosis. (haemochrom.de)
Tenascin1
Sedimentation in a cesium chloride gradient gave a higher buoyant density for human tenascin than for fibronectin, suggesting that it has a higher degree of glycosylation. (duke.edu)
Subunit1
TSP1 is a calcium-sensitive, disulfide-bonded trimer with a subunit molecular mass of 150 kDa. (bioporto.com)
Ligand1
Activity Assay: A representative lot of the original monoclonal antibody has been shown to convert alpha5 beta1 integrin into a ligand competant form and promotes fibronectin binding. (ximbio.com)
Fibrillogenesis1
SLLISWD Sequence in the 10FNIII Domain Initiates Fibronectin Fibrillogenesis. (mit.edu)
Epidermal1
The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. (nih.gov)
Tandem2
Crystal structure of tandem type III fibronectin domains from Drosophila neuroglian at 2.0 A. (embl.de)
Mature mouse IL-22BP consists of two tandem Fibronectin type-III domains and shares 85% and 68% amino acid sequence identity with rat IL-22BP and the short isoform of human IL-22BP, respectively (6, 14). (rndsystems.com)
Sequence1
The tenth fibronectin type III repeat contains a RGD cell recognition sequence in a flexible loop between 2 strands. (embl.de)
Fragment1
Previous studies have shown that small beads coated with FN7-10, a four-domain cell adhesion fragment of fibronectin, bind to cell surfaces and translocate rearward. (biologists.com)
Domain4
The second conserved disulfide bridge links the C-terminal adjacent strands of the domain. (wikipedia.org)
2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region. (embl.de)
Unlike the Ig domain, however, the FN3 lacks a disulfide bond but is highly stable. (nih.gov)
Therefore, the intact structure of the eighth fibronectin domain of PTPRJ is critical for its localization in plasma membrane and biological function. (unina.it)
Bond3
It consists of two antiparallel beta-sheets, first a double-stranded one, that is linked by a disulfide bond to a triple-stranded beta-sheet. (wikipedia.org)
This fold is topologically very similar to that of Ig-like domains, with a notable difference being the lack of a conserved disulfide bond in FN3 domains. (embl.de)
with two heavy chain and two light chain connected by disulfide bond. (sigmaaldrich.com)
Precipitation1
Fibronectin is also responsible for the precipitation of factor VIII and von Willebrand factor in the cold and for the formation of cryoprecipitates in certain diseases. (haemochrom.de)
Identification1
Schwarzbauer, JE 1991, ' Identification of the fibronectin sequences required for assembly of a fibrillar matrix ', Journal of Cell Biology , vol. 113, no. 6, pp. 1463-1473. (princeton.edu)
Cell2
Corbett SA, Lee L, Wilson CL, Schwarzbauer JE, Covalent cross-linking of fibronectin to Fibrin is required for maximal cell adhesion to a fibronectin-fibrin matrix. (haemochrom.de)
Dox conjugate 13 containing disulfide linkage showed comparable cytotoxicity versus Dox after 72 h incubation in all the cancer cell lines, whereas it was found to be less cytotoxic on RWPE-1, suggesting that it can act as a Dox prodrug. (chapman.edu)
Amino1
It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). (bio-techne.com)
Human2
Miekka SI, Ingham KC, Menache D, Rapid methods for isolation of human plasma fibronectin. (haemochrom.de)
In these studies the migration of human and mouse melanoma on fibronectin was inhibited by purified T4 and HAP1 bacteriophage preparations. (biomedcentral.com)
Plasma3
Mosesson MW, Amrani DL, Review : The structure and biological activities of plasma fibronectin. (haemochrom.de)
Quitt M, Froom P, Aghai E, Balan E, Hornstein L. Plasma fibronectin levels in patients with coronary artery disease. (haemochrom.de)
Dynamic structure of plasma fibronectin. (haemochrom.de)
Factor1
When fibrin is formed, fibronectin adheres to fibrin and is covalently linked by cross-linking by factor XIII. (haemochrom.de)