BuffersKineticsHydrogen-Ion ConcentrationProtein BindingDissociative DisordersGuanine Nucleotide Dissociation InhibitorsBinding Sitesrho-Specific Guanine Nucleotide Dissociation InhibitorsTromethamineMolecular Sequence DataSpectrometry, FluorescenceMacromolecular SubstancesTemperatureBinding, CompetitiveProtein ConformationSpectrometry, Mass, Electrospray IonizationAmino Acid SequenceCattleThermodynamicsCalciumModels, MolecularOsmolar ConcentrationMolecular WeightMathematicsMagnesiumModels, ChemicalChromatography, GelAdenosine TriphosphateEscherichia coliElectrophoresis, Polyacrylamide GelRabbitsMass SpectrometryIonsTime FactorsHEPESElectronsPeptidesCell MembraneLigandsRecombinant ProteinsModels, Biologicalrho Guanine Nucleotide Dissociation Inhibitor alphaProtein DenaturationCircular DichroismDimerizationProtein Structure, TertiarySolubilityChromatography, High Pressure LiquidSpectrophotometry, UltravioletHydrolysisCell LineTandem Mass SpectrometryGuanosine DiphosphateSurface Plasmon ResonanceGuanosine TriphosphateSpectrophotometryDNAEdetic AcidFluorescent DyesStructure-Activity RelationshipOxidation-ReductionPhosphatesMutationPeptide FragmentsCarrier ProteinsAdenosine DiphosphateDrug StabilityMolecular StructureBase SequenceSubstrate SpecificityGasesProteinsIndicators and ReagentsProtonsPhosphorylationCatalysisSolutionsHot TemperatureGuanidineOxygenUreaCalorimetryCells, CulturedEgtazic AcidChemistryDose-Response Relationship, DrugDialysisUltracentrifugationAmino AcidsChemical PhenomenaBacterial ProteinsReceptors, Cell SurfaceCytosolEnzyme ActivationChromatography, AffinityCentrifugation, Density GradientElectrophoresis, CapillaryCationsCations, DivalentHemoglobins