• The disruption of these equilibria can result in an uncontrolled stroma degeneration (as involved in scarring) or uncontrolled proliferation of epithelial cells (as in malignant melanoma). (unina.it)
  • Migration and proliferation of parenchymal and connective tissue cellsBasement membrane crucial to wound healing: collagen in the bm binds to cells via proteins like integrins, fibronectin and laminin. (medicinembbs.org)
  • In order to eliminate the interference from the transplanted cells themselves, fetal stem cells were used for transplantation because the proliferation and differentiation potentials of fetal stem cells are superior to the adult ones ( 8 ). (ijbs.com)
  • T cell activation subsequent stimulation with antigen-activated anti-CD3/Compact disc28 or APCs?antibodies, mainly because demonstrated by decreased IL-2 proliferation and secretion. (nsdfu.org)
  • The goal of the task was to regulate how physiological power EFs impact Compact disc4+ T cell activation (IL-2 secretion and proliferation) and polarisation (Th personal cytokine secretion and transcription element activation) in shaping immune system responses, also to determine the systems that exert such results. (nsdfu.org)
  • Results EF publicity suppresses activation and proliferation of activated T cells Human being lymphocytes have already been demonstrated previously to migrate towards the cathode upon contact with EFs3,4. (nsdfu.org)
  • Holographic live cell imaging, fluorescent microscopy, and gene and protein assays were used to analyze various parameters such as proliferation patterns, protein expressions and distributions. (phiab.com)
  • B-cell proliferation and affinity maturation toward locally displayed autoantigens have been demonstrated at these sites, known as tertiary lymphoid structures (TLS). (cnrs.fr)
  • For instance, enterocytes damaged by pathogens, the microbiota of aging flies, or host-derived reactive oxygen species (ROS), are replaced under the control of multiple pathways by the compensatory proliferation of intestinal stem cells (ISCs). (cnrs.fr)
  • Among the pathways identified, we showed that the JAK-STAT signaling pathway controls host defense in the gut by regulating stem cell proliferation and thus epithelial cell homeostasis. (cnrs.fr)
  • HP75 cells treated with overexpressed miR-543 exhibited increased cell proliferation, migration and invasion, while decreased cell apoptosis as well as expression of Cleaved caspase-3 and Cleaved caspase-8 were observed. (cancerindex.org)
  • during this study, we tend to incontestable that PVT1 expression levels have an effect on the proliferation and migration ability of bladder cancer cells. (journalgazett.com)
  • Moreover, PVT1 knockdown considerably attenuate the proliferation capability of bladder cancer cells in nude mice. (journalgazett.com)
  • The present work introduces a computational implementation of some principles of a theory of organism development, namely that the default state of cells is proliferation and motility, and includes the principle of variation and organization by closure of constraints. (nature.com)
  • In the present model, the bioelectric context of cells and tissue is the field responsible for organization, as it regulates cell proliferation and the level of communication driving the system's evolution. (nature.com)
  • Starting from a depolarized (proliferative) cell, the organism grows to a certain size, limited by the increasingly polarized state after successive proliferation events. (nature.com)
  • [2] These pathologies lead to a disequilibrium between proangiogenic and antiangiogenic factors that can result in the proliferation and migration of vascular endothelial cells into the corneal stroma. (aao.org)
  • In mammals, Akt is ubiquitously expressed and is associated with regulation of cellular proliferation, metabolism, cell growth and cell death. (biomedcentral.com)
  • Functional disruption of Smed-Akt alters the balance between cell proliferation and cell death leading to systemic impairment of adult tissue renewal. (biomedcentral.com)
  • This study defined the role of Smed-Akt in abnormal cell proliferation triggered by the abrogation of the phosphatase PTEN, an upstream component of the Akt signaling pathway, which is highly mutated in human cancers. (biomedcentral.com)
  • The adhesion of tumor cells to vascular endothelial cells is an important process of tumor metastasis. (bvsalud.org)
  • Studies have shown that tumor could educate vascular endothelial cells to promote tumor metastasis through many ways. (bvsalud.org)
  • However, the effect of tumor cells on the functions of vascular endothelial cells-derived extracellular vesicles (H-EVs) and the mechanisms underlying their effects in tumor-endothelium adhesion in metastasis remain mysterious. (bvsalud.org)
  • In this study, we found that H-EVs promoted the adhesion of triple negative breast cancer cell to endothelial cells and cirGal-3 enhanced the adhesion-promoting effects of H-EVs. (bvsalud.org)
  • The underlying mechanism was related to the upregulation of glycolysis in endothelial cells induced by cirGal-3 which led to the increase of the ICAM-1 expression and its transmission to MDA-MB-231 cells by H-EVs. (bvsalud.org)
  • Malignant epithelial cancers, the major cause of cancer morbidity and mortality, arise in organs composed of both epithelial and mesenchyme-derived stromal cells, such as fibroblasts, myofibroblasts, endothelial cells, pericytes, smooth muscle, and hematopoietic cells. (aacrjournals.org)
  • Wounded fibroblasts, glandular cells, and endothelial cells were also seen in mechanically stressed skin [ 4227 ]. (nanomedicine.com)
  • The percentage of rat aortic endothelial cells found to be naturally wounded varies considerably between individual animals from 1.4-17.9% (mean 6.5%) [ 3923 ]. (nanomedicine.com)
  • Wounded endothelial cells are heterogeneously distributed, being found in distinct clusters either in the shape of streaks aligned with the long axis of the vessel or in the shape of partial or complete rims surrounding bifurcation openings such as the ostia of the intercostal arteries [ 3923 ]. (nanomedicine.com)
  • These are characterized by segregated areas of prevalent T- or B-cell aggregation, differentiation of high endothelial venules, and local activation of resident stromal cells, including lymphatic endothelial cells. (cnrs.fr)
  • When ocular inflammation occurs, corneal epithelial and endothelial cells, macrophages and certain inflammatory cells produce angiogenic growth factors, namely vascular endothelial growth factor (VEGF) and fibroblast growth factors. (aao.org)
  • VEGF paves the way for new blood vessel formation by upregulating matrix metalloproteinases production by endothelial cells in the limbal vascular plexus. (aao.org)
  • In conclusion, our results suggest that intraepithelial and alveolar mast cell release of proteases plays a crucial role in epithelial homeostasis, and that an inbalance of the protease release may be involved in respiratory disease progression and in disruption of critical epithelial functions. (phiab.com)
  • Living organisms can cope with disruption of homeostasis such as injury and disease to a certain degree. (riken.jp)
  • While the process is well recognized, we still do not know the mechanisms defining responses to disruption of homeostasis in different organs and species. (riken.jp)
  • Our research focuses on three fundamental questions targeting major disruption of homeostasis in organisms: 1) How do animals repair tissues upon injury? (riken.jp)
  • They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
  • The system reaches homeostasis, with a depolarized core (proliferative cells) surrounded by a rim of polarized cells (non-proliferative in this condition). (nature.com)
  • This theory would deal with the way an organism spontaneously grows from a single cell to a complete and complex organism, introducing organization at the different development stages and homeostasis for a mature organism. (nature.com)
  • Using RNA deep sequencing, we found that raltegravir specifically boosts the expression of anti-angiogenic factors and promotes metabolic homeostasis in FHV-1-infected cells. (microbiologyresearch.org)
  • The large number of macrophages in the intestinal tract, play a significant role in maintaining the homeostasis of microorganisms on the surface of the intestinal mucosa and in the continuous renewal of intestinal epithelial cells. (frontiersin.org)
  • Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
  • The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)
  • Inflammatory immune cells recruited at the site of chronic inflammation form structures that resemble secondary lymphoid organs (SLO). (cnrs.fr)
  • The EMT also can be triggered by inflammation in adult tissues to transform epithelial cells into stem-like mesenchymal cells that aid wound healing. (scripps.edu)
  • They are thought to play a role in host defenses against infections, inflammation, wound repair, and acquired immunity. (lookformedical.com)
  • The condition affects stem cell function contributing to poor wound healing and low grade chronic inflammation. (scirp.org)
  • In addition, the effects of macrophages in the occurrence and development of inflammatory bowel disease (IBD), and their role in inducing fibrosis, activating T cells, reducing colitis, and treating intestinal inflammation were also reviewed in this paper. (frontiersin.org)
  • Aswell as traveling directional cell migration, EFs Carteolol HCl have already been shown to impact cellular functions, such as for example improved phagocytosis in macrophages and neurite development during advancement15,16. (nsdfu.org)
  • The internalized ventral cells give rise to the mesoderm that differentiates into muscle and other internal cell types such as the fat body, macrophages or lymph glands. (nature.com)
  • Besides the secretion of pro-inflammatory cytokines (TNF-alpha, IL-1beta, and IL-6), the increased expression of receptor repertoire (pattern recognition receptors, PRR) on APCs, mostly macrophages and dendritic cells, occurs as a result of the release of endogenous and exogenous antigens (damage-associated molecular patterns, DAMPs, and pathogen-associated molecular patterns, PAMPs). (hindawi.com)
  • Macrophages, which are functional plasticity cells, have the ability to phagocytize and digest foreign substances and acquire pro-(M1-like) or anti-inflammatory (M2-like) phenotypes according to their microenvironment. (frontiersin.org)
  • On the other hand, phagosomes are formed when the pathogen is engulfed by macrophages and fuse with lysosomes to release enzymes and toxic substances, resulting in killing or having cytotoxic effects on bacteria and tumor cells. (frontiersin.org)
  • The lamina propria of the small intestine is the main site of the intestinal immune system, which contains a large number of macrophages, CD4 T cells, and dendritic cells. (frontiersin.org)
  • Alveolar epithelial cells (AEC) are injured during infection that results in lung injury by disruption of the cellular barrier. (nih.gov)
  • Bronchial and alveolar remodeling and impaired epithelial function are typical characteristics of chronic respiratory diseases. (phiab.com)
  • This thesis aimed to investigate the impact of mast cell proteases on bronchial and alveolar remodelling. (phiab.com)
  • Human bronchial and alveolar epithelial cells were treated with tryptase and chymase. (phiab.com)
  • By monitoring in real time the movement of alveolar epithelial cells (an important cell type that form alveoli) in slices of lung tissue, we found that cells from the Vangl2 Lp/+ mutant mice had slower and more restricted movement compared to cells from normal healthy mice. (lungdevelopmentandrepair.org)
  • After transplantation, wounds closed as early as 8 days and were positive for α-smooth muscle actin (α-SMA), implicating the enhanced re-epithelialization and wound contraction. (ijbs.com)
  • During Drosophila gastrulation, actomyosin contraction in ventral cells generates a long, narrow epithelial furrow, termed the ventral furrow, in which actomyosin fibres and tension are directed along the length of the furrow. (nature.com)
  • Forces that shape cells and tissues can be produced by the contraction of actin filament (F-actin) meshworks by the molecular motor Myosin II (myosin). (nature.com)
  • Grafting may be used to accelerate healing and reduce insensible fluid loss from burns and other wounds, reduce scar contraction, and enhance cosmesis. (medscape.com)
  • Invasion and metastasis of malignant epithelial cells into normal tissues is accompanied by adaptive changes in the mesenchyme-derived supporting stroma of the target organs. (aacrjournals.org)
  • Analysis of chemically distinct conjugates, resistance models, and biomarkers implicates a unique mode of action, with mitotic arrest and apoptosis of malignant epithelial cells coupled to disruption of fibroblastic and vascular structures. (aacrjournals.org)
  • in turn, the malignant epithelial cells can undergo reversible epithelial-mesenchymal transitions ( 1 - 5 ). (aacrjournals.org)
  • Basal cell carcinomas (BCC) represent the most frequent malignant cutaneous tumor, the location of which always determines the surgical treatment to employ. (coek.info)
  • In the Drosophila thorax, opposing gradients of Dachsous and Four-jointed expression, which are constituents of the Fat/Dachsous/Four-jointed planar cell polarity pathway, result in the polarized localization of the myosin Dachs 9 . (nature.com)
  • In the Drosophila embryo, apical constriction in a strip of epithelial cells along the ventral midline results in the folding of the tissue and the internalization of ventral cells, forming a ventral furrow (VF). (nature.com)
  • An evolutionarily ancient role for Plexins during epithelial repair in Drosophila and zebrafish. (riken.jp)
  • article{ferrandon_complementary_2013b, title = {The complementary facets of epithelial host defenses in the genetic model organism Drosophila melanogaster: from resistance to resilience}, author = {Dominique Ferrandon}, doi = {10.1016/j.coi.2012.11.008}, issn = {1879-0372}, year = {2013}, date = {2013-02-01}, journal = {Curr. (cnrs.fr)
  • One consequence of the epithelial barrier may be the establishment of an all natural trans epithelial voltage difference that comes from the polarised distribution and practical variant of ions, ion pumps and ion stations on either family member part from the epithelial cells. (nsdfu.org)
  • Tryptase induced cell growth, cell survival, and wound healing, whereas chymase reduced cell growth, altered cell morphology and impaired epithelial barrier proprties. (phiab.com)
  • Biofilms often persist in the periphery of an actual wound, beneath an intact, healthy skin layer and the difficulty of their treatment is largely due to the outermost layer of the skin, the stratum corneum, being a natural barrier for drug delivery. (eurekalert.org)
  • Restoration of an intact barrier is of critical importance following wounding and may be achieved in numerous ways, including grafting. (medscape.com)
  • The collagen and laminin of basement mb, and the adhesive glycoprotein of the ECM like fibronectin and laminin hold the cells via integrins in the cell mb. (medicinembbs.org)
  • Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)
  • The corneal stroma is composed of primarily collagen fibrils, small leucine-rich proteoglycans (SLRPs), as well as sparsely distributed cells called keratocytes. (molvis.org)
  • The purpose of this study is to examine the therapeutic efficacy of human umbilical cord mesenchymal stem/stromal cells (UMSCs) in treating congenital and acquired corneal opacity associated with the loss of collagen V. (molvis.org)
  • Experimental mice, i.e., wild-type, Col5a1 f/f and Kera-Cre/Col5a1 f/f ( Col5a1 ∆st/∆st , collagen V null in the corneal stroma) mice in a C57BL/6J genetic background, were subjected to a lamellar keratectomy, and treated with or without UMSC (10 4 cells/cornea) transplantation via an intrastromal injection or a fibrin plug. (molvis.org)
  • Interestingly, the relationship between cell migration, differentiation marker and ECM production and remodeling during repair process followed the same in vivo timing. (unina.it)
  • Advances in bioengineering have addressed problems of how to control growth and differentiation of stem cells using novel biomaterials, how to sort and purify specific stem cell products, and how to deliver and sustain stem cell grafts in vivo. (ca.gov)
  • Dorsomorphin dihydrochloride also inhibits bone morphogenetic protein (BMP) type I receptors (ALK2, ALK3 and ALK6), promotes cardiomyogenesis in mouse embryonic stem cells (ESCs) in vitro and promotes neural differentiation of hPSCs as part of a chemical cocktail. (tocris.com)
  • These intradermal epithelial structures, such as sebaceous glands, sweat glands, and hair follicles, are lined with epithelial cells with the potential for division and differentiation. (medscape.com)
  • Recurrent corneal erosion (RCE) syndrome is a condition that is characterized by a disturbance at the level of the corneal epithelial basement membrane, resulting in defective adhesions and recurrent breakdowns of the epithelium. (medscape.com)
  • Management of RCE syndrome is usually aimed at regenerating or repairing the epithelial basement membrane to restore the adhesion between the epithelium and the anterior stroma. (medscape.com)
  • Recurrent corneal erosions and epithelial basement membrane dystrophy are usually bilateral and are characterized by various patterns of dots, parallel lines that mimic fingerprints, and patterns that resemble maps, which appear in the epithelium. (medscape.com)
  • Accidental injuries that breach the seal across epithelial levels e.g. wounding or physical disruption from the bronchial epithelium generate a localized endogenous EF that takes on a pivotal part in the recovery process8C13. (nsdfu.org)
  • EFs have already been assessed at sites where in fact the epithelium can be disrupted straight, and T cells can be found e.g. (nsdfu.org)
  • Epidermal appendages serve an important role as a source of epithelial cells, which accomplish re-epithelialization when the overlying epithelium is removed or destroyed in cases such as partial-thickness burns, abrasions, or split-thickness skin graft harvesting. (medscape.com)
  • Hu G, Liu P, Feng J, Jin Y. Transplantation with Bone Marrow Stromal Cells Promotes Wound Healing Under Chemotherapy through Altering Phenotypes. (ijbs.com)
  • Herein we characterized human fetal bone marrow stromal cells (hBMSCs) during wound healing in mice treated with cyclophosphamide (CTX). (ijbs.com)
  • Altered gene expression in these nontransformed stromal cells provides potential targets for therapy. (aacrjournals.org)
  • These and other observations have led to research into how applied electric fields can impact wound healing An increase in wound healing rate is regularly observed and this is thought to be due to the cell migration and other signaling pathways that are activated by the electric field. (wikipedia.org)
  • Treatment for this condition remains difficult and largely ineffective, because the growth of cells in the wound is inhibited ( 2 ) and chemotherapy usually leads to myelosuppression, the latter of which further compromises the migration of multipotent cells from the bone marrow ( 3 ). (ijbs.com)
  • Using our EF-cell migration experimental process and EF advantages of 50 and 150?mV/mm, just like those within wounded pores and skin9,14 or airway epithelia8, we confirmed the responsiveness of T lymphocytes for an applied EF and therefore their striking preferential cathodal migration (Fig.?1a and Supplementary Video?1). (nsdfu.org)
  • in comparison, migration of non-EF-exposed control cells didn't show a substantial directional choice and migrated arbitrarily. (nsdfu.org)
  • The directedness of migration (adverse value shows cathodal migration) was skewed seriously by EF exposures of 50 and 150?mV/mm in comparison to cells lacking any EF (50?mV/mm, ?0.85? (nsdfu.org)
  • Directed migration was therefore marked, the appearance was presented with because of it of Carteolol HCl practically all cells shifting along a straight line represented from the EF vector. (nsdfu.org)
  • The speed of T cell migration was improved significantly by EF excitement Carteolol HCl also, raising by 3-fold and by an extraordinary 6-fold at 50 and 150?mV/mm respectively (50?mV/mm, 21.44? (nsdfu.org)
  • During embryonic morphogenesis, wound repair and cancer invasion, cells often migrate collectively via tight cell-cell junctions, a process named collective migration. (biomedcentral.com)
  • During such migration, cells move as coherent groups, large cell sheets, strands or tubes rather than individually. (biomedcentral.com)
  • One unexpected finding regarding collective cell migration is that being a "multicellular structure" enables cells to better respond to chemical and physical cues, when compared with isolated cells. (biomedcentral.com)
  • This is important because epithelial cells heal wounds via the migration of large sheets of cells with tight intercellular connections. (biomedcentral.com)
  • In this review, we will briefly introduce the role of collective cell migration in wound healing, regeneration and cancer invasion and discuss its underlying mechanisms as well as implications for wound healing. (biomedcentral.com)
  • Collective cell migration is now recognized as a hallmark of tissue-remodeling events. (biomedcentral.com)
  • Indeed, virtually all living tissue is constructed by collective cell migration, which plays an important role in the initial symmetry-breaking and leader-follower organization of cell groups during embryonic development. (biomedcentral.com)
  • 4 ] Collective migration has also been recognized as an important mechanism for wound healing and cancer invasion (see details below). (biomedcentral.com)
  • 6 ] In the commonly used epithelial cell line MDCK, cells in a monolayer migrated directionally to the anode in an electric field (EF), whereas isolated cells displayed random migration in an EF of the same strength [ Figure 1 ]. (biomedcentral.com)
  • Filopodia formation mediated by receptor tyrosine kinase Ror2 is required for Wnt5a-induced cell migration. (riken.jp)
  • In this study, Dr Sek Shir Cheong discovers how a mutation in the VANGL2 gene ( Vangl2 Lp ), in mutant mice, interferes with normal mechanosignalling and directed cell migration which contribute to abnormal formation of alveoli, the site of gas exchange in the lungs. (lungdevelopmentandrepair.org)
  • DEFENSINS found in azurophilic granules of neutrophils and in the secretory granules of intestinal PANETH CELLS. (lookformedical.com)
  • Endurance exercise can disturb intestinal epithelial integrity, leading to increased systemic indicators of cell injury, hyperpermeability, and pathogenic translocation. (edu.au)
  • This study examined whether a 6-day dietary intervention influenced perturbations to intestinal epithelial disruption in response to a 25-km race walk. (edu.au)
  • During both trials, venous blood was collected pre-, post-, and 1 hr postexercise and analyzed for markers of intestinal epithelial disruption. (edu.au)
  • 05). A short-term LCHF diet increased intestinal epithelial cell injury in response to a 25-km race walk. (edu.au)
  • These cells play a key role in early resistance to intestinal pathogens. (frontiersin.org)
  • The majority of cutaneous squamous cell carcinomas (XC) are located in the head-neck-region and especially on the midface and ears. (coek.info)
  • This accounts for the remarkable ability of the face to re-epithelialize even the deepest cutaneous wounds. (medscape.com)
  • Under normal conditions, there is a balance between cell types via cell-cell contact and the extracellular matrix (ECM). (unina.it)
  • This model was composed by a cell-synthesized and responsive extracellular matrix that resembles the in vivo dermis, and it was used as living platform to study in vitro skin alteration and diseases. (unina.it)
  • Aggregate of microorganisms in which cells that are frequently embedded within a self-produced matrix of extracellular polymeric substances (EPSs) adhere to each other and/or to a surface. (wikipedia.org)
  • [2] [3] These adherent cells become embedded within a slimy extracellular matrix that is composed of extracellular polymeric substances (EPSs). (wikipedia.org)
  • [2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)
  • The enzyme metalloproteinase degrades the cornea's basement membrane and extracellular matrix, while proteolytic enzymes allow vascular epithelial cells to enter the stromal layer of the cornea. (aao.org)
  • Graft versus host disease (GVHD), a severe immunogenic complication of allogeneic hematopoietic stem cell transplantation (HSCT), represents the most frequent cause of transplant-related mortality (TRM). (hindawi.com)
  • Allogeneic hematopoietic stem cell transplantation (HSCT) offers the only curative modality for many hematological disorders. (hindawi.com)
  • MicroRNA-543 promotes cell invasion and impedes apoptosis in pituitary adenoma via activating the Wnt/β-catenin pathway by negative regulation of Smad7. (cancerindex.org)
  • To gain insight into the molecular regulation I analysed the composition of whole internalised desmosomes and the potential cross- talk with the actin cytoskeleton and actin-linked cell-cell junction proteins. (manchester.ac.uk)
  • To elucidate the molecular mechanisms of the actomyosin crosstalk with desmosome, I depleted cells of proteins that could provide the potential link of desmosomes to the actomyosin machinery (α-catenin, β-catenin). (manchester.ac.uk)
  • Traditional anti-mitosis drugs target proteins such as microtubules that are basic features of the cell division apparatus," Bollong said. (scripps.edu)
  • In addition we found a reduction in key proteins responsible for mechanosignalling within the lung epithelial cells. (lungdevelopmentandrepair.org)
  • Hyperbaric oxygen therapy (HBOT) has been shown to improve wound healing through induction of stem cell recruitment and the potential to inhibit progression of diabetic complications. (scirp.org)
  • Blood glucose, pancreatic β-cell damage, diabetic nephropathy and wound healing progression were assessed. (scirp.org)
  • Thus, the microenvironment and the stroma play an important role in both wound healing process and tumour development. (unina.it)
  • The first model of skin alteration dealt with the wound healing process, by exploiting its self-repairing capability. (unina.it)
  • Stem cell transplantation is a promising strategy for delayed wound healing caused by chemotherapy. (ijbs.com)
  • Taken together, our results indicate hBMSCs can accelerate wound healing under chemotherapy through altering their phenotypes. (ijbs.com)
  • One is delayed or impaired wound healing, which frequently occurs in the patients undergoing surgery due to long-term chemotherapy ( 1 ). (ijbs.com)
  • Previous studies reported that old patients had high risks in chemotherapy ( 9 ) and the wound healing was compromised with the increase of age ( 10 ). (ijbs.com)
  • Recent studies have gained some mechanistic insights that will benefit the clinical understanding of wound healing in general. (biomedcentral.com)
  • In essential physiological processes including morphogenesis, wound healing and tissue regeneration, cells often move as a tightly or loosely associated cohesive group. (biomedcentral.com)
  • Lastly, we tested a drug WNT5A, a molecule that belongs to the same pathway as VANGL2, and showed that it could improve wound healing ability in the mutant cells, suggesting that WNT5A could be a potential target for lung repair. (lungdevelopmentandrepair.org)
  • Diabetes leads to widespread complications including pancreatic β-cell damage, nephropathy and impaired wound healing. (scirp.org)
  • We aimed to determine the efficacy of HBOT in wound healing and organ preservation in a diabetic rat model. (scirp.org)
  • Similarly HBOT induced wound healing and accelerated epithelial closure from 5 days of HBOT. (scirp.org)
  • There was significant reduction of hyperglycemia and inhibition of diabetic complications in the form of preservation of pancreatic and kidney structure and accelerated wound healing. (scirp.org)
  • It is applied once to the wound to aid healing and regeneration of skin cells. (helsinki.fi)
  • Evaluate infection using bacterial cultures from poorly healing wounds or wounds that appear infected. (medscape.com)
  • Fibroblast cell therapy enhances initial healing in recessive dystrophic epidermolysis bullosa wounds: results of a randomized, vehicle-controlled trial. (medscape.com)
  • Thus, it is critical to understand how cells orient the cytoskeleton to produce forces that deform tissues. (nature.com)
  • Together, our in vivo and in silico data provide a framework for understanding how cells orient force generation, establishing a role for geometrical and mechanical patterning of force production in tissues. (nature.com)
  • Plasma membrane disruptions appear to be a common occurrence in cells residing in tissues such as gut and skin that are normally exposed to mechanical stress in vivo [ 4227 ]. (nanomedicine.com)
  • Mesenchymal cells are one of the major cell types in developing embryos, and ultimately give rise to bone, muscle, fat, and certain other tissues. (scripps.edu)
  • Whether they originate from EMT-transformed epithelial cells or from mesenchymal tissues, mesenchymal cancer cells are prone to form deadly metastases and are generally difficult to treat with drugs-surgical removal is often the only good option. (scripps.edu)
  • Further lab-dish tests showed that FiVe1 irreversibly blocks mitosis in several other EMT-transformed cancer cell lines, as well as in tumor cells originating from muscle, fat, cartilage and other mesenchymal tissues. (scripps.edu)
  • The neoblasts are the only dividing cells in planarians and constantly proliferate to repair tissues and support systemic cellular turnover [ 21 ]. (biomedcentral.com)
  • Their down-regulation is a key step during epithelial to mesenchymal transition (EMT), during which cells become more migratory. (manchester.ac.uk)
  • In their study, published in the Proceedings of the National Academy of Sciences , the TSRI researchers showed that the new compound, FiVe1, blocks the growth of tumor cells that have undergone what researchers call the epithelial-mesenchymal transition (EMT), a process common in breast, colon, lung and other epithelial cell-derived tumors-known as carcinomas. (scripps.edu)
  • The compound blocks cell division (mitosis) by binding to a structural protein, vimentin, that is produced abundantly in mesenchymal-type cells. (scripps.edu)
  • The EMT occurs naturally in early development to turn some epithelial cells-another broad cell type-into more free-ranging mesenchymal cells. (scripps.edu)
  • Follow-up molecular biology studies revealed that the compound blocks mitosis in the FOXC2-expressing cancer cells by binding to a structural protein called vimentin, produced principally in mesenchymal cell types. (scripps.edu)
  • FiVe1's selectivity for vimentin-containing mesenchymal cancer cells means that it wouldn't have the same side-effects as traditional chemotherapy drugs. (scripps.edu)
  • The cells express FOXC2, a transcription factor gene that promotes the EMT and makes cancer cells more stem-like, more prone to metastasis and more resistant to treatment. (scripps.edu)
  • Innate immunity: wounds burst H2O2 signals to leukocytes. (riken.jp)
  • Exercise causes membrane damage in muscle cells (e.g., rat muscle fiber cells or myocytes [ 4229 ]) and red blood cells ( Section 15.5.5.1.1 ), and dystrophic muscle cells are especially susceptible [ 4230 ]. (nanomedicine.com)
  • In May 2023, the US Food and Drug Administration approved Vyjuvek (beremagene geperpavec), a topical gene therapy drug, for wound treatment in dystrophic epidermolysis bullosa with COL7A1 mutations. (medscape.com)
  • Prevent infection and disruption of wound. (physio-pedia.com)
  • It maintains optimal moisture balance while protecting the wound site from contamination and infection. (helsinki.fi)
  • They belong to the intermediate filament molecules and play an important role in maintaining the structural integrity of epithelial cells. (coek.info)
  • [8] Steroids are thought to work by inhibiting cell chemotaxis and by inhibiting pro-inflammatory cytokines like interleukin-1 and -6, as well as by also causing lymphocyte death and inhibiting vascular dilation, which all amounts to their antiangiogenic effect. (aao.org)
  • The ECM provides structural scaffolding for cells, as well as contextual information. (unina.it)
  • We have used stroma-rich models in immunodeficient mice, which recapitulate the histotypic arrangement found in human epithelial cancers. (aacrjournals.org)
  • Breast cancers are most often epithelial tumors involving the ducts or lobules. (msdmanuals.com)
  • General references Breast cancers are most often epithelial tumors involving the ducts or lobules. (msdmanuals.com)
  • As a fact, carcinogenesis can disrupt these forms of communication, thus altering cell biology of human skin. (unina.it)
  • Consequently, we investigated the role of skin cells and BM components on melanoma biology and invasive ability in reconstructed human skin equivalent. (unina.it)
  • We have developed and renovated a 1400 sq. ft. shared-use stem cell laboratory called the Laboratory for Stem Cell Biology and Engineering. (ca.gov)
  • Past and current users of the Laboratory for Stem Cell Biology and Engineering include 52 researchers from 18 research groups, representing collaborations with 12 companies and 17 different universities and research institutes. (ca.gov)
  • Past and current users of the Laboratory for Stem Cell Biology and Engineering include 58 researchers (6 new researchers in the past year) from 18 research groups, representing collaborations with 12 companies and 17 different universities and research institutes. (ca.gov)
  • She serves on NIH study sections and as reviewer for journals in her area of expertise, manages an active laboratory, supervises students in pulmonary biology and epithelial cell research and has received awards recognizing both teaching and original research accomplishments. (rochester.edu)
  • Our new research article "The Planar Polarity Component VANGL2 Is a Key Regulator of Mechanosignaling" has been published in Frontiers in Cell and Developmental Biology. (lungdevelopmentandrepair.org)
  • Thus, Akt signaling regulates neoblast biology and mediates in the distribution of injury-mediated cell death during tissue repair in planarians. (biomedcentral.com)
  • The junctions between cells maintain "supracellular" structures and result in collective polarization, intercellular force generation, decision-making and eventually, tissue organization. (biomedcentral.com)
  • In one proposal [ 4233 ], MAb-complexed ferrofluid particles selectively bound to the surfaces of virus-infected cells would be rapidly vibrated using an external magnetic field, causing the bound particles to perforate the cell membranes of the infected cells or to damage their intracellular structures, leading to targeted cell lysis. (nanomedicine.com)
  • Early redox, Src family kinase and calcium signaling integrate wound responses and tissue regeneration in zebrafish. (riken.jp)
  • Planarians are flatworms with large populations of stem cells capable of dividing to support adult tissue renewal and regeneration. (biomedcentral.com)
  • Our results also reveal novel roles for Akt signaling during regeneration, specifically for the timely localization of cell death near the injury site. (biomedcentral.com)
  • This paucity is likely due to the ubiquitous nature of this signaling pathway and the difficulty of analyzing stem cells in their natural environment during physiological cell turnover and regeneration in conventional animal models [ 15 - 17 ]. (biomedcentral.com)
  • The microcystic epithelial vesicles may break, causing foreign body sensations and severe pain with more extensive corneal epithelial disruption. (medscape.com)
  • 1 ] In the zebrafish lateral line primordium, a migrating cluster of approximately two hundred cells migrate, grow, divide and differentiate simultaneously and assemble into a series of connected epithelial rosette-like mechanosensory organs. (biomedcentral.com)
  • The magnitude, direction and timing of contractile force depend on the organization of the cellular actomyosin meshworks and how these networks are connected between cells at the level of the tissue. (nature.com)
  • This process revealed that the Vangl2 Lp mutation caused disruption of the actomyosin network (a kind of scaffold present in cells that is analogous to the musculoskeletal system in the human body), as well as focal adhesions, which function as molecular clutches, enabling cells to attach to their surrounding matrix. (lungdevelopmentandrepair.org)
  • One study [ 4227 ] produced an estimate that the epidermis of digits from actively locomoting animals is composed of 10.5% wounded cells, vs. 3.7% wounded cells for quiescent animals. (nanomedicine.com)
  • Additional causes of bacterial infected wounds include traumatic injuries, as well as diabetic foot ulcers, venous leg ulcers, and pressure ulcers. (eurekalert.org)
  • It is used as a wound dressing for burn injuries and skin grafts. (helsinki.fi)
  • All of these abnormalities leads to the the cells being floppy as they are unable to generate force, thereby affecting their ability to move or migrate which is particularly important during the process of lung formation or lung repair after injury. (lungdevelopmentandrepair.org)
  • Interestingly, this finding is strikingly similar to published in vivo data from carcinomas and wound edges. (manchester.ac.uk)
  • Correction of junctional epidermolysis bullosa by transplantation of genetically modified epidermal stem cells. (medscape.com)
  • A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
  • The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
  • Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)
  • [10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)
  • Subpopulations of cells within the biofilm differentiate to perform various activities for motility, matrix production, and sporulation, supporting the overall success of the biofilm. (wikipedia.org)
  • In several cases, we found the ionic liquid was more efficacious on a biofilm than a standard bleach treatment and exhibited minimal cytotoxicity effects on human cell lines (unlike bleach). (eurekalert.org)
  • This has excellent prospects for aiding antibiotic delivery to the pathogen through biofilm disruption but, most interestingly, the ionic liquids themselves are quite effective for pathogen neutralization," Fox said. (eurekalert.org)
  • Further, many bacterial infestations in wounds penetrate under the outer skin layer, the stratum corneum, and deep into the tissue (epidermis and dermis). (eurekalert.org)
  • The hair follicle is another important source of epithelial cells, and many of the other dermal appendages actually open into the hair follicle rather than directly onto the skin. (medscape.com)
  • A subset of Xenopus neural crest (NC) cells respond efficiently to the chemoattractant SDF1, whereas isolated cells barely respond. (biomedcentral.com)
  • Cytotoxic T lymphocytes were determined as the cellular effectors of GVHD, and the key role of antigen-presenting cells (APCs) in T-lymphocyte activation was established during the following years [ 11 , 12 ]. (hindawi.com)
  • Comparison of cellular mechanics in wildtype and Vangl2 Lp cells. (lungdevelopmentandrepair.org)
  • If AMPK switch isn't turned on, energy production declines, cellular function gets gummed up with cell trash and stagnation. (wellnessresources.com)
  • Nonetheless, there is limited understanding of how Akt signaling controls the response of stem cells during cellular turnover and tissue injury in the complexity of the whole organism. (biomedcentral.com)
  • Cell viability assay also revealed that the extracts could safely be used in concentrations of 125 µg/mL. (bvsalud.org)
  • Lyn is a redox sensor that mediates leukocyte wound attraction in vivo. (riken.jp)
  • Taken together, these results indicate that raltegravir has profound and specific effects on the host transcription profile of herpesvirus-infected cells that may contribute to the overall antiviral activity of the drug and could provide therapeutic benefits in vivo . (microbiologyresearch.org)
  • Scientists at The Scripps Research Institute (TSRI) have discovered a compound that in laboratory tests irreversibly stops the growth of certain aggressive, treatment-resistant tumor cells. (scripps.edu)
  • Researchers have become aware in just the last decade that breast, colon and other epithelial-derived tumor cells sometimes exploit the EMT to detach from a primary tumor and acquire stem-like properties. (scripps.edu)
  • Vasoconstriction followed by vasodilatation and an influx of inflammatory mediators and white blood cells. (physio-pedia.com)
  • For now, it is thought that redistribution of membrane-bound sensors dragged by Coulombic forces and electro-osmosis at the membrane would cause the cell to polarize, then migrate. (wikipedia.org)
  • However, physical exercise (running) and spontaneous hypertension may not produce an increased frequency of aortic endothelial cell membrane wounding [ 3923 ]. (nanomedicine.com)
  • For example, passing cells back and forth through a standard syringe needle or similar narrow orifice causes transient membrane disruptions [ 4232 ], and each trip through a 14-gauge blood dialysis needle at a 2.2 m/sec peak velocity damages 0.1% of red cells near the needle wall [ 3690 ]. (nanomedicine.com)
  • The diversity of biological cells and environmental conditions make it likely that there are many different mechanisms that allow for cells to migrate due to electric fields. (wikipedia.org)
  • Some researchers have indicated that cells move passively without any specific sensing mechanisms applied to alter active motility. (wikipedia.org)
  • The method of detection of a field in mammalian cells is under active investigation and might involve several mechanisms. (wikipedia.org)
  • Research carried out in the facility has made significant, high impact contributions in three areas of stem cell research: Molecular Mechanisms/Basic Science, Translational Bioengineering, and Regenerative Medicine. (ca.gov)
  • However, the mechanisms by which Akt signaling regulates stem cell behavior in the complexity of the whole body are poorly understood. (biomedcentral.com)
  • Other research reports suggest that bacteria cells might perceive local electric fields via chemotaxis. (wikipedia.org)
  • Biofilms are a major cause of chronic wounds and wound degeneration. (eurekalert.org)