Discovery epitope mapping. Medical search. Frequent questions

Epitope binning is referenced in the literature under different names such as epitope mapping and epitope characterization. (wikipedia.org)
Our featured services are Epitope Mapping, Bioanalysis and Analytical Characterization. (novabioassays.com)
Specific focus will be on epitope mapping and characterization experiments captured in the Immune Epitope Database (IEDB) which heavily utilizes OBI. (bioontology.org)
Rodda, S.J. (2002) Peptide Libraries for T-Cell Epitope Screening and Characterization. (mimotopes.com)
High throughput allergen characterization is often based on phage display technique which is limited by the constraints of a prokaryotic expression system such as potential loss of conformational epitopes and lack of post-translational modifications. (bvsalud.org)
Binding kinetics, affinities, and cross-reactivity to orthologs or protein family members of large antibody panels combined with epitope binning enable rapid characterization of hundreds of antibody-antigen interactions and epitope coverage. (carterra-bio.com)
From kinetics to epitope characterization, HT-SPR has become an indispensable technology for the characterization of biotherapeutics. (carterra-bio.com)
Binding characterization assays are a critical component of the early discovery process. (carterra-bio.com)

Epitope binning is a competitive immunoassay used to characterize and then sort a library of monoclonal antibodies against a target protein. (wikipedia.org)
Antibodies against a similar target are tested against all other antibodies in the library in a pairwise fashion to see if antibodies block one another's binding to the epitope of an antigen. (wikipedia.org)
Closely related binning profiles indicate that the antibodies have the same or a closely related epitope and are "binned" together. (wikipedia.org)
In a typical example, a library of monoclonal antibodies to a small protein, thought to recognize linear epitopes, was screened using a PepSet of biotinylated peptides (Figure 2). (mimotopes.com)
Using this approach, the linear epitopes of several monoclonal antibodies were quickly and clearly identified. (mimotopes.com)
identified new anti-CD3 antibodies from humanized rats that bind to multiple epitopes and elicit varying levels of T-cell activation. (antibodysociety.org)
Detailed epitope mapping showed that the epitope recognized by Sym021 was unique compared to the clinically approved PD1 antibodies pembrolizumab and nivolumab. (antibodysociety.org)
With now almost 400 unique antibodies, CoVIC has mapped the epitope landscape on the SARS-CoV-2 Spike protein using the Carterra LSA platform. (carterra-bio.com)
In my presentation, I will show examples of how we use HTP SPR in our workflow of characterizing therapeutic monoclonal antibodies and how HTP SPR combined with a mutagenesis approach was used to identify the epitopes of a two-mAb mixture binding CD40. (carterra-bio.com)

This review reports a comprehensive update of the current scientific knowledge about proteins and peptides of MAP with already documented humoral response. (degruyter.com)
In addition, deep epitope discovery becomes feasible creating tens of thousands of low-cost peptides for functional cellular, binding, mass spectrometric and various other assays. (jpt.com)
For instance, in epitope mapping experiments, peptides should contain between 9 and 22 amino acids because epitopes typically fall within that size range. (proteogenix.science)
Antibody epitope mapping by ELISA is simple to perform using biotinylated peptides because the peptides can be captured onto Streptavidin™ or NeutrAvidin™ coated plates through the strong noncovalent avidin-biotin binding. (mimotopes.com)
T-cell epitopes (Helper and Cytotoxic) are easily located using PepSets provided peptides of the correct length are used2. (mimotopes.com)
We at CNAM have studied the immune response of infected or control patients (Covid-19, asymptomatic, or pre-epidemic sera) by epitope mapping against peptides derived from the structural peptides of SARs-CoV-2. (jpt.com)
By using overlapping 15mer peptides both the IE1 and pp65 antigens were mapped and new epitopes are described that could be used in the construction of peptide vaccines. (ucl.ac.uk)

We offer T-cell assays for T cell vaccine target identification, deep T cell epitope discovery, neo-epitope qualification, T-cell epitope mapping and antigen discovery. (jpt.com)
Here, we briefly introduce the principles, advantages, and disadvantages of the latest epitope mapping techniques with examples for viral antigen discovery. (bvsalud.org)

Venue: :Days 1 and 2, [http://www.universityinn.com/ Ramada Inn, UB North Campus, Buffalo] :Day 3: [http://www.hwi.buffalo.edu/about_hwi/visitor/maps_directions.html, Hauptmann-Woodward Institute, Buffalo] '''Goals''' Provisional goals of the meeting are: To identify and coordinate activities on-going in immunology ontology and related fields, with special attention to the use of ontologies to support clinical data analysis in flow cytometry and other fields. (bioontology.org)
Mapping the location of these specific epitopes has become a hot topic in immunology and immune therapy. (bvsalud.org)

This eBook explores advances in biosensor technology and instrumentation that can significantly accelerate antibody discovery through efficient quantitation, kinetics and epitope binning. (technologynetworks.com)
There are companies and universities that provide SPR services such as drug discovery, epitope mapping and kinetics. (sprpages.nl)

It can be used for high resolution epitope mapping, immune monitoring and biomarker discovery in vaccine development and enables the investigation of specific immune response in similar viruses. (jpt.com)
It is widely used in peptide library screening for drug discovery, target validation, epitope mapping, vaccine development, and structure-activity studies. (novoprolabs.com)
NIS is expanding with new services and locations to support its growing customer base requiring cryo-EM for drug discovery and vaccine development applications. (nanoimagingservices.com)

For our antibody mapping studies we have used the JPT's PepStar peptide microarrays and their comprehensive microarray profiling services. (jpt.com)
Kinase Profiling Services,Epitope Mapping Services,Phosphopeptide Binding and Protein Binding Studies. (pristinebiomedical.com)
Transcriptomics : MicroRNA Discovery & Profiling Services,Small RNA Discovery & Profiling Services,Transcriptome Sequencing etc. (pristinebiomedical.com)

Serimmune is an immune intelligence company that decodes the breadth and complexity of human antibody response, empowering discovery projects through a universal serology assay that identifies disease-specific epitopes across proteins and entire proteomes. (serimmune.com)
Almost at the same time, reverse vaccinology (RV) emerges as one of the applied approaches to assess the genomic sequences for prediction of novel candidate proteins and their immunogenic epitopes which may elicit protective immune responses [ 7 , 8 ]. (biomedcentral.com)
Though the group did the mapping with ABC transporters from a yeast species - Saccharomyces cerevisiae - many of the proteins are homologous to disease-linked human ABC proteins. (genomeweb.com)
Overall, the study yielded a map covering 537 unique binary interactions across 366 proteins, the study authors wrote, which they annotated with functional classifications to create a standard map, as well as a map showing protein conservation in humans and known disease associations. (genomeweb.com)

1] Ansari, H. R., Raghava, G. P., Identification of conformational B-cell Epitopes in an antigen from its primary sequence. (degruyter.com)

stands for the identified residues via mutagenesis experiments, and stands for the key residues which were predicted by … Mechanical H-bonding stabilization is another mark for key interaction residues across 10B12/GPVI complex interface Similar Ibudilast to the thermal stabilization reflected by the survival ratios of H-bonds, the mechanical stabilizations of H-bonds was utilized to map paratope and epitope residues [21] also. (ap26113.com)
Of the above nine residues proposed through mechanical stabilization, LYS41 had been identified as an epitope residue by mutagenesis experiments, and others had not. (ap26113.com)
Mapping paratope to epitope via H-bond stabilization index To score synthetically the thermal and mechanical stabilization of a hydrogen bonding, hydrogen bond stabilization Ibudilast index (HBSI), which was presented in our previous work for antithrombotic antibody 6B4 [21], were calculated by the average of the mean survival ratio and the normalized mean rupture time of each of all detected bond (see Methods section). (ap26113.com)

Epitope Binning is used in the discovery and development of new therapeutics, vaccines, and diagnostics. (wikipedia.org)
mapping and designing novel vaccines. (helmholtz-hzi.de)
The complexity and diverse range of epitopes are essential to study and map for the diagnosis of disease, the design of vaccines and for immunotherapy. (bvsalud.org)

We will also shortly discuss how leveraging single cell multi-omic approaches can be used to accelerate cellular biomarker discovery during clinical trials to predict response to therapy, follow responsive cell types, and define novel druggable target pathways. (frontiersin.org)

In this study, the researchers applied the two aforementioned strategies: tandem affinity purification with the SFB ( S-protein , FLAG epitope, and streptavidin-binding peptide) tag, as well as proximity labeling by using a second-generation biotin ligase, BioID2. (news-medical.net)

Rapid Novor develops cutting-edge proteomics services, including SPR, protein sequencing, HDX-MS epitope mapping, and antibody discovery via polyclonal antibody sequencing. (sprpages.nl)

γ interferon expression in CD8+ T cells is a marker for circulating cytotoxic T lymphocytes that recognize an HLA A2-restricted epitope of human cytomegalovirus phosphoprotein pp65. (bdbiosciences.com)

As advances continue to be made in screening technologies, the use of labels in traditional drug discovery screening assays has been criticized as producing undesirable and unanticipated interactions that can compromise screening data and lead to false conclusions. (drugdiscoverynews.com)
In December 2009, we reported that Caliper Life Sciences entered into an agreement with SRU Biosystems to use its label-free BIND technology to offer new functional assays as part of its discovery alliances and services. (drugdiscoverynews.com)
In April 2010, we reported that ForteBio chose Tecan as its preferred automation partner for real-time, label-free assays to support bioprocess and drug discovery workflows. (drugdiscoverynews.com)
Our customized peptide libraries with flexible layout and specifications are optimized for deep T-cell epitope discovery using ELISpot and intra-cellular cytokine staining assays. (jpt.com)
We will describe practical guidelines for designing, executing and analyzing epitope binning assays using Carterra's LSA and industry-leading Epitope™ software. (carterra-bio.com)
This presentation will focus on recently developed assays that expand the versatility of HT-SPR in the biotherapeutic space as well as move into new areas drug discovery. (carterra-bio.com)

The development of new potential diagnostic strategies for the immunodetection of pathogens is closely linked to the discovery of small polypeptides with immunogenic or immunoreactive activity. (degruyter.com)

Regardless of the naming, epitope binning is prevalent in the pharmaceutical industry. (wikipedia.org)
All applications in biotherapeutic discovery and development such as epitope mapping and binning and antibody screening are impossible, or at least highly cumbersome, with labeled techniques. (drugdiscoverynews.com)

This application is summarized in a separate article entitled Truncated Peptide Libraries for Cytotoxic T-Cell Epitope Mapping, available on the Mimotopes website. (mimotopes.com)

Lead antibody discovery using phage display and other technologies. (surfsearch.org)

We offer a discovery service to academia, government and industry through the Serimmune universal serology platform that utilizes bacterial display peptide library technology and next generation sequencing to broadly profile antibody repertoires and identify antigens and epitopes associated with many diseases - all in a single assay. (serimmune.com)

Our fast, cost-effective, high throughput peptide synthesis platforms enable discovery of vaccine targets from a variety of antigens or even pathogenic proteomes in indications such as infectious diseases and cancer. (jpt.com)
Recently, epitope mapping techniques have evolved to become multiplexed, with the advent of high-throughput sequencing and techniques such as bacteriophage-display libraries and deep mutational scanning. (bvsalud.org)
We combined the high-throughput of mass cytometry, facilitating the analysis of 57 twin pairs, with cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) of eight selected twin pairs to obtain a comprehensive overview of epitopes, transcriptome and T cell receptor (TCR) clonotypes. (nature.com)

The site will have a significant impact and support the growing community of private companies using cryo-EM as part of their drug discovery programs," said Holly Souter, Silicon Therapeutics. (nanoimagingservices.com)

The great debate on the use of in vivo versus in vitro sources and strategies for antibody discovery and generation continues to thrive among antibody research groups. (rapidnovor.com)

CD8+ lymphocytes respond to different HIV epitopes in seronegative and infected subjects. (bdbiosciences.com)

Among them, 42 could be produced and characterized for solubility, oligomerization state, thermal stability, epitope exposure and structural properties. (europa.eu)
An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites for the activation of adaptive immunity. (bvsalud.org)

Serimmune's Serum Epitope Repertoire Analysis (SERA) technology platform applies bacterial display peptide libraries, next-generation sequencing, machine learning and custom bioinformatics to reveal the many diverse antigens stimulating immunity. (serimmune.com)

and Philippe Mourere, head of sales, marketing and business development for Caliper Discovery Alliances & Services (CDAS). (drugdiscoverynews.com)
The project has been structured in 3 main pathways or "tracks": a Discovery track, aiming at designing gp41-based antigens (Ag) using an iterative improvement process, and two development tracks (Parenteral and Mucosal) to determine the safety and immunogenicity of the Ags and the optimal administration conditions before selecting the most promising candidate for clinical development. (europa.eu)
We provide a suite of services supporting therapeutic protein/antibody/ADC discovery, development and IND filing. (novabioassays.com)
The group hopes the map, published online this week in Nature Chemical Biology , will be a resource for future applied research, providing insight into these molecules' roles in a variety of cellular processes with implications for drug development and other clinical areas. (genomeweb.com)
With flexible workflows designed to support the needs of pharma and biotech clients, NIS infrastructure and expertise will provide affordable, reliable and rapid solutions to support discovery and development. (nanoimagingservices.com)
We defined early phase research as basic or discovery research (T0-T1) and late phase research as applied and implementation research, surveillance, and development of evidence-based guidelines (T2-T4). (cdc.gov)
Additionally, papers that could apply to multiple topic areas, and did not have a primary focus in any single topic above, were placed in the "other" category.Early phase translation (T0-T1) studies correspond to basic and discovery studies, while Late phase translation (T2-T4) studies report on surveillance, applied and implementation research and the development of evidence-based guidelines. (cdc.gov)

SpotMix Pools are optimal for broad antigen target discovery, deep T-cell epitope discovery and biological screening. (jpt.com)

To fulfill this vision, we created a technology for building and interpreting a dynamic map of human immunity which continually evolves to map interactions between functional antibody repertoire and human disease. (serimmune.com)
Researchers from the University of Toronto have created a map of the protein interactions of 19 members of the ATP-binding cassette transporter class in a species of yeast, combining this data with previously-reported ABC transporter interactions into a comprehensive "interactome. (genomeweb.com)
In the group's ABC transporter study, Stagljar and his team used MYTH to map the interactions of all of S. cerevisiae's 19 non-mitochondrial ABC transporters. (genomeweb.com)
Important for the map's potential usefulness in future clinically-oriented research, 50 percent of the interactions mapped involve a target with an identifiable human ortholog, about 40 percent of which are known to be associated with human disease. (genomeweb.com)
The group went on in its recent study to investigate two potentially interesting associations revealed in the yeast ABC transporter interactome map: physical interactions of ABC transporters with one another and the interaction of these molecules with members of the zinc transport system. (genomeweb.com)

These atlases build an ideal scientific discovery environment for reference and data mining approaches, which often times reveals new cellular disease networks. (frontiersin.org)
The engineered TCR-NK technology in Syena is based on the scientific discoveries of Katy Rezvani, M.D., Ph.D., professor of Stem Cell Transplantation & Cellular Therapy at MD Anderson. (businesswire.com)

JPT offers the largest portfolio of peptide formats for the effective and deep discovery, confirmation, and qualification of T-cell antigen targets, immunodominant epitopes and neo-epitopes. (jpt.com)
T-cell epitope mapping by ﬂow cytometry. (bdbiosciences.com)
CMV epitope selection and its use in T cell reconstitution in patients undergoing haematopoietic stem cell transplantation. (ucl.ac.uk)

Stagljar said that his team is interested in following up on some insights from the map that are promising for human pharmaceutical research. (genomeweb.com)

Custom synthesized biotinylated PepSets for linear antibody mapping are designed by the research scientist with help from experienced Technical Consultants at Mimotopes, and are shipped either ready to redissolve and plate or as ready-to-use plates already coated with the peptide (Figure 2). (mimotopes.com)
Conclusions The present computer strategy combining homology modeling, rigid body docking and MD simulation was illustrated to be effective in mapping paratope on antithrombotic antibody 10B12 to epitope on GPVI, and have large potential in drug discovery and antibody research. (ap26113.com)

Industry and academic scientists presented new paradigms in discovery, applications and workflows including HT-SPR. (carterra-bio.com)

We will provide tips and tricks for leveraging the power of our software to groom a data set, remove unnecessary complexity, and apply community analysis while retaining sufficient nuance to most accurately describe the epitope landscape. (carterra-bio.com)

Label-free technologies enable drug discovery researchers to monitor a wide array of target types that play a role in the study of diseases such as diabetes, obesity, cancer, inflammation, neuromuscular disorders, pathological pain and psychiatric disorders. (drugdiscoverynews.com)
They harnessed that method in this recent study to build the yeast ABC transporter map. (genomeweb.com)

paratuberculosis (MAP) represents a good example of the potential of this discovery-phase. (degruyter.com)
JURA Bio's highly differentiated TCR discovery platform has transformative potential and combines the power of synthetic biology with that of machine learning. (businesswire.com)

Another design strategy was developed using gammaretroviruses backbone where 2 domains were substituted with HIV epitopes in the N-terminal part of the ectodomain and the membrane proximal external region (MPER) respectively. (europa.eu)

Numbers displayed on the map are a representation of the activity in each country. (washington.edu)

Anatomy8

Organisms8

Chemicals and Drugs45

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes30

Disciplines and Occupations3

Information Science5

Health Care1

Characterization8

Antibodies9

Peptides7

Antigen discovery2

Immunology2

Kinetics2

Vaccine development3

Profiling Services3

Proteins4

Conformational1

Stabilization3

Vaccines3

Biomarker discovery1

Affinity1

Proteomics1

Pp651

Assays6

Immunogenic1

Binning2

Cytotoxic1

Phage display1

Peptide Library1

High throughput3

Therapeutics1

Vitro1

Lymphocytes1

Structural2

Sera1

Development7

Screening1

Interactions5

Cellular2

Cell3

Pharmaceutical1

Research2

Workflows1

Complexity1

Study2

Potential2

Design1

Representation1