Differentiation histone acetyl acetyltransferase p300 cbp transcription factors. Medical search. Frequent questions

Factors that bind to TAZ2 are also potentially regulated via acetylation due to its proximity to the acetyltransferase domain (KAT). (wikipedia.org)
For example, increasing nuclear acetyl-CoA levels increases histone acetylation and administration of class I histone deacetylase (HDAC) inhibitors increases longevity through increased histone acetylation. (etsu.edu)
ACSS2 nuclear translocation can result in increased activity of CREB binding protein (CBP), p300/CBP-associated factor (PCAF), and other HATs to increase histone acetylation on the promoter of neuroprotective genes including transcription factor EB (TFEB) target genes resulting in increased lysosomal biogenesis and autophagy. (etsu.edu)
These studies have identified evolutionary conserved roles for histone acetylation in promoting longevity. (etsu.edu)
Future studies should focus on the role of nuclear acetyl-CoA and histone acetylation in the control of hypothalamic inflammation, an important driver of organismal aging. (etsu.edu)
In addition, Zyflamend up regulated the histone acetyl transferase complex CBP/p300, potentially contributing to the increase in histone 3 acetylation. (biomedcentral.com)
Our results suggest that the extracts of this polyherbal combination increase histone 3 acetylation, inhibit the expression of class I and class II histone deacetylases, increase the activation of CBP/p300 and inhibit cell proliferation, in part, by up regulating p21 expression. (biomedcentral.com)
Acetylation of the N-terminal tails of histones "relaxes" the chromatin making it more accessible for binding by co-activating factors. (biomedcentral.com)
Cell viability/proliferation assays, cell cycle analysis by flow cytometry, immunodetection of specific histone and p53 acetylation marks, western blotting, siRNA and RT-qPCR. (biomedcentral.com)
In addition to its effects on histone modifications, garcinol was found to block CBP/p300-mediated acetylation of the C-terminal activation domain of p53, but resulted in enhanced acetylation of p53K120, and accumulation of p53 in the cytoplasmic compartment. (biomedcentral.com)
They also modify transcription factors and CHROMATIN through ACETYLATION. (childrensmercy.org)
Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. (cancerindex.org)
Modulators and inhibitors are used in enzyme inhibition assays to study histone acetylation, or the addition of an acetyl group on a histone, which then affects the regulation of transcription through chromatin remodeling. (epigentek.com)
Certain epigenetic modulators and inhibitors specific to histone acetylation studies have been shown to inhibit histone acetyltransferases (HATs) such as p300/CBP associated factor histone acetyltransferase, inhibit the transcription factor NF kappa B, promote apoptosis thr. (epigentek.com)

however, while these proteins share many structural features, emerging evidence suggests that these two co-activators may promote transcription of genes with different biological functions. (wikipedia.org)
Post-translational modifications (PTMs) of histones and other proteins are perturbed in tumours. (biomedcentral.com)
Enzymes that modify chromatin and associated proteins by the addition or removal of acetyl or methyl groups play a key role in genome regulation [ 1 ]. (biomedcentral.com)
Histone modifications act as signals that are 'read' by sensory proteins containing bromodomains, PHD fingers and other domains, many of which function as coregulators of DNA-binding transcription factors [ 4 ]. (biomedcentral.com)
CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. (novusbio.com)
These epigenetic mechanisms are mediated by chemical modifications of DNA, proteins (most commonly histones), and RNA. (activemotif.com.cn)
Histone demethylation is defined as the removal of methyl groups from histone proteins by histone demethylases (KDMs), which affects the inactivation of chromatin and therefore gene expression. (epigentek.com)
Histone methylation is described as the transfer of methyl groups to histone proteins (commonly H3 and H4) by histone methyltransferases (HMTs), which affects the activation of chromatin and therefore gene expression. (epigentek.com)
and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. (lookformedical.com)
This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. (lookformedical.com)

In this study we asked whether global epigenetic mechanisms regulate timing of progenitor cell differentiation into myelin-forming oligodendrocytes in vivo. (rupress.org)
Unlike sequence-specific transcription factors, epigenetic regulators do not necessarily bind DNA at consensus sequences, but still achieve reproducible target binding in a manner that is cell and maturation-type specific. (frontiersin.org)
A growing body of evidence indicates that epigenetic regulators rely, amongst other factors, on their interaction with untranslated RNA molecules for guidance to particular targets on DNA. (frontiersin.org)
To date, most of our understanding of epigenetic regulation and its participation in leukemogenesis is based on the enzymatic activities and protein-protein interactions of histone and DNA modifiers. (frontiersin.org)
However, a growing body of evidence suggests that recruitment, stability, and function of epigenetic factors can be mediated by non-protein-coding RNAs. (frontiersin.org)
Post-translational modification of histones, in particular the removal or addition of acetyl groups on ϵ-N-acetyl lysine residues, play an important role in epigenetic regulation of transcription. (biomedcentral.com)
This effect is accompanied by epigenetic changes involving histone post-translational modifications at the mineralocorticoid receptor (MR) gene and decreased MR expression in the hippocampus. (nature.com)
This article provides an introduction to the world of epigenetics, covers the history of this field in biology, discusses the factors and players that play a role in this process, and highlights which biological processes are influenced by epigenetic mechanisms. (activemotif.com.cn)

The bromodomain of CBP binds to regions of the genome are rich with acetylated lysine residues, meaning they have lost their positive charge, decreasing histone affinity for DNA, which makes the region more open and accessible for transcription. (wikipedia.org)
The 380 protein residue lysine acetyltransferase (KAT) domain is arguably one of the most important and identifying structural components of CBP. (wikipedia.org)
Histone deacetylation modulators and inhibitors inhibit histone deacetylases (HDACs) also called lysine deacetylases (KDACs). (epigentek.com)
Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each. (lookformedical.com)

Histone deacetylation was essential during a specific temporal window of development and was dependent on the enzymatic activity of histone deacetylases, whose expression was detected in the developing corpus callosum. (rupress.org)
Administration of VPA after myelination onset had no effect on myelin gene expression and was consistent with changes of nucleosomal histones from reversible deacetylation to more stable methylation and chromatin compaction. (rupress.org)
Modulators and inhibitors are used in enzyme inhibition assays to study histone deacetylation, or the removal of an acetyl group on a histone, which impacts the regulation of DNA expression. (epigentek.com)
A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)

A number of natural and synthetic molecules that inhibit histone acetyltransferase (HAT) or histone deacetylase (HDAC) activities have been described. (biomedcentral.com)

The current experiments were designed to investigate the effects of Zyflamend on the expression of class I and II histone deacetylases, a family of enzymes known to be over expressed in a variety of cancers. (biomedcentral.com)
Histone deacetylases (HDACs) are a family of enzymes associated with cancer risk. (biomedcentral.com)
Garcinol treatment alters expression of chromatin modifying enzymes in MCF7 cells, resulting in reprogramming of key histone and p53 PTMs and growth arrest, underscoring its potential as a cancer chemopreventive agent. (biomedcentral.com)
Thus, drug-like molecules that target chromatin modifying enzymes to reprogram selected histone PTMs in tumour cells may have potential as cancer chemopreventive agents. (biomedcentral.com)
Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones. (lookformedical.com)

CWR22Rv1 cells, a castrate-resistant prostate cancer cell line, were treated with Zyflamend and the expression of class I and II histone deacetylases, along with their downstream target the tumor suppressor gene p21, was investigated. (biomedcentral.com)
Zyflamend down-regulated the expression of all class I and II histone deacetylases where Chinese goldthread and baikal skullcap (two of its components) appear to be primarily responsible for these results. (biomedcentral.com)
Expression of the tumor suppressor gene p21, a known downstream target of histone deacetylases and CBP/p300, was increased by Zyflamend treatment and the effect on p21 was, in part, mediated through Erk1/2. (biomedcentral.com)

It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. (novusbio.com)

The protein encoded by this gene associates with p300/CBP. (novusbio.com)
The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. (cancerindex.org)

In these diseases, association of CBP with β-catenin has been shown to promote cancer cell proliferation and disease aggressiveness, whereas p300/ β-catenin leads to cell differentiation and/ or apoptosis. (wikipedia.org)
Histone phosphorylation has been implicated in numerous processes such as transcription, DNA repair, cell cycle progression and apoptosis, and chromosome condensation. (epigentek.com)

It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. (novusbio.com)

In summary, although garcinol and curcumin can both inhibit histone acetyltransferase activities, our results show that these compounds have differential effects on cancer cells in culture. (biomedcentral.com)
Histone demethylation modulators and inhibitors inhibit histone demethylases (HDMs) and offer insight into diseases such as breast cancer, prostate cancer, and lung cancer that are related to histone demethylation. (epigentek.com)
Histone methylation modulators and inhibitors inhibit histone methyltransferases and allow researchers to study diseases, especially cancer, that are related to histone methylation. (epigentek.com)
These histone phosphorylation modulators and inhibitors act to inhibit proteases and phosphatases for research investigating diseases connected to phosphorylation such as Alzheimer's and Huntington's disease. (epigentek.com)

During the first 10 postnatal days, administration of valproic acid (VPA), the specific inhibitor for histone deacetylase activity, resulted in significant hypomyelination with delayed expression of late differentiation markers and retained expression of progenitor markers. (rupress.org)
A maternal high-fat diet modulates fetal SIRT1 histone and protein deacetylase activity in nonhuman primates. (childrensmercy.org)
It has histone deacetylase activity and represses transcription when tethered to a promoter. (cancerindex.org)

CREB-binding protein, also known as CREBBP or CBP or KAT3A, (where CREB is cAMP response element-binding protein) is a coactivator encoded by the CREBBP gene in humans, located on chromosome 16p13.3. (wikipedia.org)
Aka KIX domain, CREB binding domain, MYB interaction domain The kinase-inducible domain (KID) interacting domain (KIX) also is the protein domain on CBP and p300 where heterodimers form from the interaction of CBP (or p300) with other transcription factors and coactivators. (wikipedia.org)
A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. (childrensmercy.org)

PCR2 targets genes that are developmentally regulated and catalyzes di- and histone tri-methylation, resulting in chromatin compaction and gene repression. (chicagobiomedicalconsortium.org)
Protein interacting with PcG histone methylation sites may provide sites for therapeutic intervention. (chicagobiomedicalconsortium.org)
The He lab identified a core complex comprised of two subunits: METTL3 and METTL14, and an accessory factor WTAP, which mediates cellular m6A RNA methylation. (chicagobiomedicalconsortium.org)
Cellular RNA methylation level can have profound impacts on normal cell differentiation and cancer cell proliferation. (chicagobiomedicalconsortium.org)

Together, these data identify global modifications of nucleosomal histones critical for timing of oligodendrocyte differentiation and myelination in the developing corpus callosum. (rupress.org)
Although treatment with curcumin, garcinol or the garcinol derivative LTK-14 hampered MCF7 cell proliferation, differential effects of these compounds on histone modifications were observed. (biomedcentral.com)

The functional CBP is approximately 7362 nucleotides long and encodes 2,441 amino acids. (wikipedia.org)

They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. (childrensmercy.org)
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. (cancerindex.org)

It is found as a subunit of protein complexes that are in involved in the enzymatic modification of histones including the Mi2 and Sin3 histone deacetylase complexes and the polycomb repressive complex 2. (lookformedical.com)

This relatively unique acetyltransferase activity is also seen in another transcription enzyme, EP300 (p300). (wikipedia.org)

Its activity is regulated via phosphorylation of CBP. (wikipedia.org)

A histone chaperone that facilitates nucleosome assembly by mediating the formation of the histone octamer and its transfer to DNA. (lookformedical.com)

Acetyl-CoA is a metabolite at the crossroads of central metabolism and the substrate of histone acetyltransferases regulating gene expression. (etsu.edu)
Class I and II HDACs form complexes with multiple cofactors for activation where histones are a primary substrate [ 20 ] and have been targets for cancer therapies, including PrC [ 21 ]. (biomedcentral.com)

CBP is also implicated in the etiologies of several other diseases including hematologic malignancies and other solid tumors, diabetes, schizophrenia, Alzheimer's disease, depression, and many other neurological conditions. (wikipedia.org)

These and other PTMs generate a combinatorial histone code that demarcates chromatin regions for transcription activation or repression [ 2 ]. (biomedcentral.com)

it is able to add acetyl groups to both transcription factors as well as histone lysines, the latter of which has been shown to alter chromatin structure making genes more accessible for transcription. (wikipedia.org)
CBP has also been shown to help modulate liver function via maintenance of energy homeostasis in response to changes in cell nutrition conditions by regulating the activity of transcription factors and genes responsible for lipogenesis and gluconeogenesis. (wikipedia.org)
Several genes involved in different aspects of stem cell activation are being studied, including some that transduce initial signals, and others that regulate self-renewal and differentiation. (chicagobiomedicalconsortium.org)

The KIX domain controls the rate of transcription and has been shown to be critical for hematopoietic differentiation. (wikipedia.org)
It is a heterogeneous disease, cellularly and molecularly, with an overarching theme of ectopic self-renewal and arrested differentiation potential at multiple levels of the hematopoietic tree ( 1 ). (frontiersin.org)
Akin to the normal hematopoietic system, leukemias are sustained by a small number of leukemia stem-like cells (LSC), which can be distinct from the normal hematopoietic stem cells (HSC) but also exhibit functional characteristics of self-renewal and (abnormal or hindered) differentiation, and are often quiescent ( 2 - 4 ). (frontiersin.org)

Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)
A retinoblastoma-binding protein that has an affinity for core HISTONES. (lookformedical.com)

It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. (cancerindex.org)

Drug-like molecules that can reprogram selected histone PTMs in tumour cells are therefore of interest as potential cancer chemopreventive agents. (biomedcentral.com)

The identification of mechanisms modulating timing of cellular differentiation is critical for morphogenesis and proper development. (rupress.org)
Emergence of LSC is dependent on individual or combined genetic mutations that broadly determine the cellular affiliation of the leukemia, and permit or impose ectopic self-renewal and a restricted differentiation potential into what constitutes the proliferative bulk of the leukemia ( 5 , 6 ). (frontiersin.org)
During the process, known as cellular differentiation, the zygote develops into pluripotent stem cells which can give rise to all of the different organs in the body. (activemotif.com.cn)

Because of this, compounds that decrease cytoplasmic acetyl-CoA have been described as CR mimetics. (etsu.edu)

The modification has been shown to be critical to cell differentiation, animal development, and a range of biological signaling and stress response. (chicagobiomedicalconsortium.org)
In this study we assessed the effects of the phytocompounds garcinol and curcumin on histone and p53 modification in cancer cells, focussing on the breast tumour cell line MCF7. (biomedcentral.com)

A number of factors that associate with CBP bind to either the CH1 or CH3 domain, or to both, despite their locations at opposing ends of the protein. (wikipedia.org)
The CH2 region, located in the middle of the protein, in its acetyltransferase domain, does not contain this consensus sequence, and has not been conclusively shown to bind zinc ions. (wikipedia.org)
e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens. (lookformedical.com)

In many tissues fasting or lifespan extending calorie restriction (CR) decreases glucose-derived metabolic flux through ATP-citrate lyase (ACLY) to reduce cytoplasmic acetyl-CoA levels to decrease activity of the p300 histone acetyltransferase (HAT) stimulating pro-longevity autophagy. (etsu.edu)
Although cytoplasmic acetyl-CoA synthetase 2 (ACSS2) promotes aging by decreasing autophagy in some peripheral tissues, increased glial AMPK activity or neuronal differentiation can stimulate ACSS2 nuclear translocation and chromatin association. (etsu.edu)

Regulatory transcription factors (TFs) are encoded by approximately 10% of the human genome [ 1 ]. (biomedcentral.com)

Timely differentiation of progenitor cells is critical for development. (rupress.org)
Histone PTMs are also critical for other genomic functions, such as DNA replication and induction of repair mechanisms at sites of DNA damage [ 3 ]. (biomedcentral.com)

A histone chaperone protein that plays a role in the deposition of NUCLEOSOMES on newly synthesized DNA. (lookformedical.com)

This hypothesis implied that OL differentiation proceeded by default once the cells exited from the cell cycle. (rupress.org)

Anatomy21

Organisms9

Chemicals and Drugs124

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes76

Disciplines and Occupations1

Information Science4

Acetylation14

Proteins10

Epigenetic8

Lysine4

Deacetylation4

HDAC1

Enzymes5

Deacetylases3

Transferase1

Protein encoded by this gene2

Apoptosis2

Vitro1

Inhibit4

Deacetylase activity3

CREB3

Methylation4

Modifications2

Amino acids1

Transcriptional2

Enzymatic1

Enzyme1

Phosphorylation1

Nucleosome1

Substrate2

Diseases1

Repression1

Genes3

Hematopoietic3

Affinity2

Regulation of transcription1

Molecules1

Cellular3

Compounds1

Modification2

Bind3

Activity2

Genome1

Critical2

Role1

Cell cycle1