Differentiation germ autologous cells cord blood stem cell transplantation. Medical search. Frequent questions

In vivo, they eventually differentiate into all of the body's cell types (making them pluripotent). (wikipedia.org)
These were termed induced pluripotent stem cells (iPSCs). (wikipedia.org)
Induced pluripotent stem cells, iPSCs, are mature, differentiated cells, such as skin cells, that are reprogrammed in the laboratory to be similar to undifferentiated embryonic stem cells. (jove.com)
That is, they divide and are pluripotent, able to produce any cell type in the body. (jove.com)
These cells are also pluripotent like embryonic stem cells-able to produce all cell types-and are therefore called induced pluripotent stem cells (iPSCs). (jove.com)
The transcription factors then turn on many other genes that are expressed by embryonic stem cells, returning the cells to an undifferentiated, proliferative, and pluripotent state. (jove.com)
Induced pluripotent stem cells, which harness the power of genetic reprogramming - basically, the altering of a cell's DNA - to change the course of cellular development. (articlecity.com)
Human induced pluripotent stem cells and male infertility: an overview of current progress and perspectives. (jsurgmed.com)
Single transcription factor efficiently leads human induced pluripotent stem cells to functional microglia. (reprocell.com)
Variation of DNA methylation on the IRX1/2 genes is responsible for the neural differentiation propensity in human induced pluripotent stem cells. (reprocell.com)
Using Microfluidics to Generate Human Naïve and Primed Pluripotent Stem Cells. (reprocell.com)
In: Rugg-Gunn P. (eds) Human Naïve Pluripotent Stem Cells. (reprocell.com)
Comparative analysis of human induced pluripotent stem cell-derived mesenchymal stem cells and umbilical cord mesenchymal stem cells. (reprocell.com)
3 Embrionic stem cells (ESCs) are derived from the inner cell mass of the blastocyst and form all cell types, derived from the three germ layers, and are therefore pluripotent. (bvsalud.org)
Specifically, about the paracrine activities of amniotic fluid stem cells, renal stem cells, embryonic stem cells, mesenchymal stem cell, induced pluripotent stem cells as well as other stem cells. (biomedcentral.com)
At first, the mesenchymal stem cell was thought to be the primary component of this undifferentiated cell type, however it is now evident that within the adipose extracellular matrix are also adipocytic precursors (known as progenitor cells) adherent to adipocytes, and in close approximation to a variety of additional undifferentiated multipotent and pluripotent cells, including pericytes and endothelial cells, all thought to play important roles in mesenchymal-stromal derived tissue regeneration. (journalofprolotherapy.com)
In particular, the advent of human embryonic stem cells followed by reprograming technologies for generation of induced pluripotent stem cells have instigated studies into modeling human brain development and disease by providing a means to simulate a human tissue otherwise completely or largely inaccessible to researchers. (ehu.eus)
The present invention provides methods to promote the differentiation of pluripotent stem cells. (patsnap.com)
The present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer. (patsnap.com)

However, when they are isolated and cultured in vitro, they can be kept in the stem-cell stage and are known as embryonic stem cells (ESCs). (wikipedia.org)
In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
In these studies, the differentiation capacities of MSCs, obtained from diverse sources, to male germ cells were determined in vitro and their effects on testis niche were assessed by injection of MSCs into the testis. (jsurgmed.com)
Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
However, the limited amount of stem cells and their tendency to undergo spontaneous differentiation upon extended propagation in vitro restrict their practical application. (biomedcentral.com)
In addition, we found that in the presence of LIF, MDLS could replace feeder cells to maintain the undifferentiated state of OG2-mES cells (Oct4-GFP reporter gene mouse embryonic stem cell line), and the MDLS-expanded OG2-mES cells showed an elevated expression levels of pluripotency markers in vitro. (biomedcentral.com)
However, one of the bottlenecks in the stem cell therapy is that stem cells tend to differentiate into other types of cells during culture in vitro. (biomedcentral.com)
Therefore, finding a way to effectively maintain the pluripotency of stem cells cultured in vitro is important for the application of stem cells. (biomedcentral.com)
Kondziolka and colleagues used N-Tera 2 cells derived from a lung metastasis of a human testicular germ cell tumour that when treated with retinoic acid generate postmitotic neurons that maintain a fetal neuronal phenotype indefinitely in vitro (LBS neurons). (bmj.com)
Un rôle majeur des prostaglandines dans l'hématopoïèse, ainsi mis en évidence, a pu être étendu à la souris chez qui un traitement par la prostaglandine PGE2 provoque la multiplication des CSH in vivo et in vitro, un résultat qui génerera très probablement des applications cliniques. (hypotheses.org)
Raji cells (a B lymphocyte line) were selected as the cell model in vitro. (bvsalud.org)
In vitro cell experiments further confirmed that JQZF can inhibit Raji proliferation and promote cell apoptosis through AKT/mTOR/c-Myc signaling pathway. (bvsalud.org)
To explore the molecular mechanism by which sorafenib regulates blood glucose levels, hepatic glucose metabolism signaling was studied by a series of in vivo and in vitro experiments. (bvsalud.org)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
Here, we describe the pre-clinical in vitro and in vivo study of irradiated haNK cells engineered to express a second-generation chimeric antigen receptor (CAR) targeting programmed death-ligand 1 (PD-L1). (elifesciences.org)

As of 2016[update], the only established medical therapy using stem cells is hematopoietic stem cell transplantation, first performed in 1958 by French oncologist Georges Mathé. (wikipedia.org)
Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)

Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
Many experiments have been conducted to determine the efficacy of MSCs in the treatment of male infertility due to their differentiation capacity and paracrine effect. (jsurgmed.com)
However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
We therefore briefly discuss differences found in subsets of human bmMSCs and in MSCs isolated from some other sources and touch upon how this could be utilized for cell-based therapies. (hindawi.com)
The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
Overview of studies published regarding MSCs using the term "stem cell" or "stromal cell" in the last 20 years accessed by a web search in July 2015 (Google Scholar). (hindawi.com)
It seems that the term "stem cell" became more popular although the "stemness" was only shown in a more strict sense for MSCs involved in osteogenesis and bone repair. (hindawi.com)
When the biological properties of MSCs were explored in more detail, questions arose whether these cells met the criterion of a true stem cell [ 8 ]. (hindawi.com)
Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)
MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
Mesenchymal stem cells (MSCs) are ASCs, and were first described in 1966 by Friedenstein et al. (bvsalud.org)
MSCs can be isolated from different locations, such as bone marrow, umbilical cord, placenta, adipose and dental tissues. (bvsalud.org)
12 Bang and colleagues reported the safety and feasibility of intravenous infusion of autologous mesenchymal stem cells (MSCs) with no reported adverse effects in five patients treated with intravenous MSCs. (bmj.com)

They are usually distinguished from progenitor cells, which cannot divide indefinitely, and precursor or blast cells, which are usually committed to differentiating into one cell type. (wikipedia.org)
they are vastly outnumbered by the progenitor cells and terminally differentiated cells that they differentiate into. (wikipedia.org)
At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
This review will examine the clinical potential of several stem and progenitor cells that may be utilised to regenerate defunct or damaged vasculature and restore blood flow to the ischaemic tissue. (biomedcentral.com)
In particular, we focus on the therapeutic potential of endothelial progenitor cells as an exciting new option for the treatment of ischaemic diseases. (biomedcentral.com)
Therefore, the therapeutic use of appropriate populations derived from stem and progenitor cells has been considered for cell-based therapies for neurodegenerative diseases, primarily Parkinson's disease, and brain injuries. (ehu.eus)

In that same year, Siminovitch was a lead investigator for studies that found colony-forming cells were capable of self-renewal, which is a key defining property of stem cells that Till and McCulloch had theorized. (wikipedia.org)
They are able to differentiate into any cell of an organism and have the ability of self-renewal. (articlecity.com)
Stem cells are defined by their capacity for self-renewal and multilineage differentiation, making them uniquely situated to treat a broad spectrum of human diseases. (articlecity.com)
To qualify as stem cells, self-renewal has to be shown as well [ 10 ]. (hindawi.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
Stem cells (SCs) are undifferentiated cells with self-renewal ability and capacity to differentiate into specialized cell types. (bvsalud.org)
The self-renewal and proliferative nature of stem cells raised the hope to fight against various diseases. (biomedcentral.com)
Octamer-binding transcription factor-4 (Oct4), a transcription factor belongs to the POU transcription factor family Class V, is fundamental for maintaining self-renewal ability and pluripotency of stem cells. (biomedcentral.com)
Colony formation assay, sphere-forming ability assay, alkaline phosphatase (AP) activity assay and teratoma-formation assay were used to assess the role of modaline sulfate (MDLS) in promoting self-renewal and reinforcing pluripotency of P19 cells. (biomedcentral.com)
Moreover, MDLS significantly promoted the self-renewal capacity of P19 cells. (biomedcentral.com)
These findings demonstrated, for the first time, that MDLS could maintain self-renewal and pluripotency of stem cells. (biomedcentral.com)

As with other types of stem cells, scientists are learning how to promote the differentiation of specific cell types from iPSCs efficiently, so that the needed cell types can be produced in adequate quantities. (jove.com)

For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
Worldwide, approximately 90,000 first HSCTs-53% autologous and 47% allogeneic-are performed every year, according to the World Wide Network of Blood and Marrow Transplantation. (medscape.com)

For example, retinal cells that could be used to replace damaged retinal tissue. (jove.com)
With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)
Current research is focused on growing a wide range of new tissue from stem cells, including muscle, blood, brain, and cartilage cells. (articlecity.com)
Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
They have many of the positive characteristics of embryonic stem cells while sourcing material is far less challenging, since postpartum tissue can be used. (articlecity.com)
Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
If the underlying ischaemia is not sufficiently resolved it can lead to tissue damage, with subsequent cell death. (biomedcentral.com)
This leads to hypoxia and tissue damage as a consequence of the build up of waste metabolites and may result in cell death [ 1 ]. (biomedcentral.com)
Cells for vascular therapy must be able to home to ischaemic or damaged tissue and engage in vessel formation alone or in unison with resident vasculature to achieve a controlled and functional reperfusion event, without causing pathological angiogenesis (for example, proliferative retinopathy in the vitreous of the eye). (biomedcentral.com)
A cell therapy approach should be aimed at promoting revascularisation of ischaemic tissue. (biomedcentral.com)
There is a therapeutic window in which to deliver the cells, to avoid extensive tissue damage, fibrosis and necrosis. (biomedcentral.com)
Due to the ability to self-renew and to differentiate into cells that are found throughout the body, there is a great interest in using stem cells for the regeneration of injured tissues as well as to develop tissue-engineered implants and bio-hybrid organs, in order to restore tissue function. (bvsalud.org)
The use of ASCs in regenerative medicine and tissue engineering research has important advantages in comparison with ESCs, since there are no ethical complications and the process of differentiation of these cells is better controlled. (bvsalud.org)
In explant method, the dental tissue is placed on a plastic surface and the cells migrate out from the tissue fragment adhering to culture flasks or dishes ( Figure 1 ). (bvsalud.org)
Stem cells and hydrogels may provide a functional, dynamic and biologically equivalent tissue that promotes tissue regeneration while being gradually degraded and replaced. (raaot.org.ar)
The standard approach to tissue engineering consists in delivering cells within a hydrogel or a three-dimensional printed biomaterial scaffold into the chondral lesion to induce regeneration. (raaot.org.ar)
Proteomics is a powerful approach to study the whole set of proteins expressed in an organism, organ, tissue or cell resulting in valuable information on physiological or pathological state of a biological system. (oaji.net)
The general underlying strategy behind the development of tissue engineered organ substitutes is the utilization of a combination of cells, biomaterials, and biochemical cues intended to recreate the natural organ environment. (biomedcentral.com)
The purpose of this review is to highlight current bioengineering approaches for salivary gland tissue engineering and the adult stem cell sources used for this purpose. (biomedcentral.com)
This article is intended to provide a review of the most current approaches to utilizing stem cells and bioengineering principles for the purpose of salivary tissue regeneration. (biomedcentral.com)
One explanation for deficient repair is when undifferentiated adult stem repair cells are inadequate in number or cannot be stimulated within the damaged tissue site. (journalofprolotherapy.com)
With improved understanding of tissue healing and regeneration, stem cell Prolotherapy is gaining significant clinical importance and potential. (journalofprolotherapy.com)
Background: Stem cells are of two types: embryonic and adult stem cells and they act as a repair system by replenishing body tissue. (benthamscience.com)
Discussion: Activation of the PI3K/AKT pathway decreases the concentration of apoptotic factors, while the HIF pathway protects stem cells against the micro-environment of tissue (hypoxia). (benthamscience.com)
Ramalingam, M. Surface functionalization of nanobiomaterials for application in stem cell culture, tissue engineering, and regenerative medicine. (benthamscience.com)
Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. (lookformedical.com)
White blood cells formed in the body's lymphoid tissue. (lookformedical.com)
Adaptive redox response of mesenchymal stromal cells to stimulation with lipopolysaccharide inflammagen: mechanisms of remodeling of tissue barriers in sepsis. (biomedfrontiers.org)
Here, we present a multimodal -omics platform for the comprehensive study of cell populations in complex tissue, which has allowed us to characterize the cells involved in wound healing across both time and space. (stanford.edu)

Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)
[ 1 ] More than half of autologous transplantations are performed for multiple myeloma and non-Hodgkin lymphoma , and the vast majority of allogeneic transplants are performed for hematologic and lymphoid cancers. (medscape.com)

This has allowed us to reveal potential mechanisms controlling fibroblast fate during migration, proliferation, and differentiation following skin injury, and thereby reexamine the canonical phases of wound healing. (stanford.edu)

Most of these lymphomas represent the malignant counterpart of B-lymphocytes at midstage in the process of differentiation. (lookformedical.com)

They exist to replenish rapidly lost cell types and are multipotent or unipotent, meaning they only differentiate into a few cell types or one type of cell. (wikipedia.org)
With high levels of platelet-derived growth factors and cytokines, this combination provides both a living bioscaffold and a multipotent cell replenishment source useful for enhanced musculoskeletal healing. (journalofprolotherapy.com)
Multipotent aşama, hücrelerin, genellikle germ tabakası kökenlerine bağlı olarak, sınırlı spesifik hücre tiplerine farklılaşma yeteneği ilekarakterize edilir (6). (adaveteriner.com)

The use of cell therapy for vascular regeneration offers an exciting new prospect in regenerative medicine. (biomedcentral.com)
Conclusion: stem cells from dental origin are an interesting alternative for research and application in regenerative therapies in Dentistry. (bvsalud.org)
8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)
It includes the possible stem cell therapy mechanisms involved and outcomes recorded so far, the limitations of using these regenerative medicines, and the progressive improvement in stem cell therapy by adopting approaches like PiggyBac, Sleeping Beauty, and the Sendai virus. (biomedcentral.com)
Stem cells have been extensively explored for a variety of regenerative medical applications and they play an important role in clinical treatment of many diseases. (biomedcentral.com)
In order to address the limits associated with current therapies, many research groups have investigated various regenerative medicine strategies utilizing different stem cell sources to engineer artificial salivary tissues that can mitigate the effects of xerostomia and hyposalivation. (biomedcentral.com)
Since their discovery, stem cells have ushered in a new era in regenerative medicine. (ehu.eus)
In particular, due to the limited regenerative capacity of the adult nervous system, the therapeutic potential of stem cells has opened up new perspectives for development of treatment strategies for brain repair after injury or disease. (ehu.eus)
Stem cell therapy aims to use special regenerative cells (stem cells) to repopulate areas of damage that result from spinal cord injuries, with the hope of improving the ability to move ("motor outcomes") and to feel ("sensory outcomes") beyond the site of the injury. (regenerativemedicine.net)

In multicellular organisms, stem cells are undifferentiated or partially differentiated cells that can differentiate into various types of cells and proliferate indefinitely to produce more of the same stem cell. (wikipedia.org)
Since 1998 however, it has been possible to culture and differentiate human embryonic stem cells (in stem-cell lines). (wikipedia.org)
However, scientists have found a way to reprogram these mature cells so that they "de-differentiate" and return to an unspecialized, proliferative state. (jove.com)
Stem cells differentiate into different types of cells, such as neural, hematopoietic, adipose, etc. and are used for the treatment of various conditions like myocardial infarction, spinal cord injury, Parkinson's disease and diabetes. (benthamscience.com)

Its name dates back to the 17th century, when the English anatomist Thomas Wharton first identified the jellylike substance that surrounds vital parts inside the umbilical cord. (articlecity.com)

The effects of JQZF on the proliferation and apoptosis of B cells were detected by flow cytometry and CCK8. (bvsalud.org)
Conclusion: For the protection of stem cells against apoptosis, different pathways, namely Phosphoinositide 3-Kinase (PI3K/AKT), Hypoxia-Inducible Factor (HIF1), Mitogen-Activated Protein Kinases (MAPK) and Hippo, are discussed. (benthamscience.com)
Cell apoptosis was analyzed by flow cytometry. (degruyter.com)
Moreover, TGFBR2 was a direct target of miR-128-3p, and its overexpression reversed the inhibitory effects of miR-128-3p overexpression on inflammation and apoptosis in LPS-induced HK2 cells. (degruyter.com)
miR-128-3p could inhibit apoptosis and inflammation by targeting TGFBR2 in LPS-induced HK2 cells, which might provide therapeutic strategy for the treatment of sepsis. (degruyter.com)
Transforming growth factor-β (TGF-β) signaling pathway is a multifunctional cytokine and plays important roles in regulating cell proliferation, cell cycle, differentiation, migration, and apoptosis in a broad spectrum of tissues [ 12 ]. (degruyter.com)

This is called autologous transplantation, and it reduces the risk of transplant rejection that can occur when tissues are transplanted between individuals. (jove.com)
Treating such diseases remains difficult and several strategies have been used to stimulate the growth of blood vessels and promote regeneration of ischaemic tissues, such as the use of recombinant proteins and gene therapy. (biomedcentral.com)
In vascular medicine, various stem cells and adult progenitors have been highlighted as having a vasoreparative role in ischaemic tissues. (biomedcentral.com)
Ischaemia is characterised by a reduction in oxygen supply to tissues and organs, usually as a result of blood vessel constriction or obstruction. (biomedcentral.com)
The evaluation of the most suitable timing of cell delivery as well as the number of cells needed to integrate into resident vasculature and promote revascularisation of specific tissues requires careful optimisation and evaluation. (biomedcentral.com)
In human postnatal dental tissues, five main sources of DSCs have been identified: dental pulp stem cells (DPSCs), 11 stem cells from human exfoliated deciduous teeth (SHEDs), 12 periodontal ligament stem cells (PDLSCs), 13 dental follicle stem cells (DFSCs) 14 and stem cells from apical papilla (SCAPs). (bvsalud.org)
Recognition of the vast number of undifferentiated cells associated with the stromal vascular fraction has resulted in extensive research demonstrating the heterogeneity of such cells, and their ability to participate in production of all mesodermal-derived tissues. (journalofprolotherapy.com)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

Research into stem cells grew out of findings by Canadian biologists Ernest McCulloch, James Till and Andrew J. Becker at the University of Toronto and the Ontario Cancer Institute in the 1960s. (wikipedia.org)
The key properties of a stem cell were first defined by Ernest McCulloch and James Till at the University of Toronto and the Ontario Cancer Institute in the early 1960s. (wikipedia.org)
The potential uses for them are vast: from basic research leading to a better understanding of the source of birth defects and cancer to potential treatments for common conditions like arthritis, diabetes, and heart disease to treatments for rarer but severe conditions like spinal cord and brain injuries. (articlecity.com)
Since the initial discovery of the self-renewing properties of stem cells in the early 1960s - pioneered by a small team of researchers at the Ontario Cancer Institute [3] - the understanding of the power and diversity of stem cells has increased rapidly. (articlecity.com)
When germ-free cell cultures became a laboratory routine, hopes were high for using this novel technology for treatment of diseases or replacement of cells in patients suffering from injury, inflammation, or cancer or even refreshing cells in the elderly. (hindawi.com)
Cancer cells thrive in a changing microenvironment by reprogramming lipidomic metabolic processes to provide nutrients and energy, activate oncogenic signaling pathways, and manage redox homeostasis to avoid lipotoxicity. (bvsalud.org)
In addition, we also discuss the existing methods of artificial mitochondrial transfer/transplantation (AMT/T) and their therapeutic effects on diseases to explore the potential applications of mitochondrial transfer and transplantation in the treatment of mitochondrial-related diseases, mitochondrial diseases, and cancer. (biomedcentral.com)
Shende, P. Role of liposome-based stem cells for multimodal cancer therapy. (benthamscience.com)
Here, we demonstrate that NK cells (haNKs) engineered to express a PD-L1 chimeric antigen receptor (CAR) haNKs killed a panel of human and murine head and neck cancer cells at low effector-to-target ratios in a PD-L1-dependent fashion. (elifesciences.org)
PD-L1 CAR haNKs reduced levels of macrophages and other myeloid cells endogenously expressing high PD-L1 in peripheral blood from patients with head and neck cancer. (elifesciences.org)
Thus, addressing the presence of immunosuppressive myeloid cell populations in the periphery and tumor microenvironment of patients with cancer is likely to be required for effective NK cell-based immunotherapy. (elifesciences.org)
Ovarian cancer stem cells are enriched in side population and aldehyde dehydrogenase bright overlapping population. (biomedfrontiers.org)
We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
3) We are defining the properties of cancer stem cells that initiate and sustain the unique disease features of acute leukemias through the use of various adoptive animal models. (stanford.edu)
Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. (patsnap.com)
In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

These cells have been studied for the treatment of periodontitis, bone repair, regeneration of the pulp after necrosis as well as the development of new teeth. (bvsalud.org)
Bach, E.A. JAK/STAT signaling in stem cells and regeneration: From drosophila to vertebrates. (benthamscience.com)

Adult stem cells are found in a few select locations in the body, known as niches, such as those in the bone marrow or gonads. (wikipedia.org)
McCulloch and Till began a series of experiments in which bone marrow cells were injected into irradiated mice. (wikipedia.org)
They observed lumps in the spleens of the mice that were linearly proportional to the number of bone marrow cells injected. (wikipedia.org)
The first therapy using stem cells was a bone marrow transplant performed by French oncologist Georges Mathé in 1958 on five workers at the Vinča Nuclear Institute in Yugoslavia who had been affected by a criticality accident. (wikipedia.org)
The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)

To create iPSCs, mature cells, like skin fibroblasts, are taken from a patient and put into culture. (jove.com)
iPSCs are potentially valuable in medicine, because a patient who needs a particular cell type-for instance, someone with a damaged retina due to macular degeneration-could receive a transplant of the required cells, generated from another cell type in their own body. (jove.com)
To create iPSCs, mature cells such as skin fibroblasts or blood cells from a person are grown in culture. (jove.com)
It is still being studied whether iPSCs are truly equivalent to embryonic stem cells, but they appear to be similar and can produce cells from all three germ layers of the body. (jove.com)

By 1998, human embryonic stem cells were first isolated by American biologist James Thomson, which made it possible to have new transplantation methods or various cell types for testing new treatments. (wikipedia.org)
Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)
Cell Reports Methods. (reprocell.com)
This, along with the development of unrelated cord blood transplantation and familial haploidentical transplantation methods, have improved the likelihood of finding an appropriate HSCT source in a timely manner. (medscape.com)
Further investigation is needed to determine specific effects of cell therapy and to optimise cell delivery methods, cell dosing, type of cells used, timing of delivery, infarct size and location of infarct that are likely to benefit from cell therapy. (bmj.com)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
Multiple research groups have developed artificial mitochondrial transfer/transplantation (AMT/T) methods that transfer healthy mitochondria into damaged cells and recover cellular function. (biomedcentral.com)
Methods: This article focuses on recent research development that addresses the viability issues of stem cells. (benthamscience.com)
The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and/or stimulate cells that maximizes stimulation and/or proliferation of such cells. (patsnap.com)
The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. (patsnap.com)
Compositions of cells activated and expanded by the methods herein are further provided. (patsnap.com)
Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided. (patsnap.com)
Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. (patsnap.com)
Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. (patsnap.com)
Methods of identifying differentiation factors capable of promoting the differentiation of endoderm cells, such as PDX1-positive foregut endoderm cells and PDX1-negative definitive endoderm cells, are also disclosed. (patsnap.com)

Immunofluorescence, RT-PCR, and western blotting were used to measure expression changes of stem-related genes and activation of related signaling pathways. (biomedcentral.com)
Failed T cell-based immunotherapies in the presence of genomic alterations in antigen presentations pathways may be overcome by NK cell-based immunotherapy. (elifesciences.org)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)

Specific properties, such as proliferative and differentiation potential, are slightly different among DSCs. (bvsalud.org)

1, 2 Recently, Prolotherapists have begun to utilize the potential of autologous adipose (fat)-derived stem/stromal cells (AD-SC) within non-manipulated fat graft scaffolding, combined with high-density PRP concentrates (HD-PRP) to provide a potent biological therapeutic combination. (journalofprolotherapy.com)
7 There has been some variation and question regarding the correct terminology for this population of stromal adipose cells. (journalofprolotherapy.com)
Therefore we have chosen to use the term "adipose-derived stem/stromal cells" (AD-SC's), rather than simply "mesenchymal stem cells. (journalofprolotherapy.com)

Then, typically, genes for transcription factors, are delivered by viral vectors into the cell nuclei, where they are incorporated into the genome. (jove.com)
The transcription factors then turn on genes that are expressed by embryonic stem cells. (jove.com)
Then, genes for multiple transcription factors are delivered into the cells using a viral vector, and the transcription factor proteins are expressed using the cell's machinery. (jove.com)
Found within Wharton's jelly - which is easily harvested from what would otherwise be post-natal medical waste - are several distinct stem cell genes. (articlecity.com)
CONCLUSION: Sorafenib reduces blood glucose levels through downregulating gluconeogenic genes, especially in diabetic mice, suggesting the patients with T2DM when treated with sorafenib need more emphasis in monitoring blood glucose to avoid unnecessary hypoglycemia. (bvsalud.org)
This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)

Organ transplantation is the ultimate treatment option for patients suffering from refractory diseases. (frontiersin.org)
So as a new therapeutic approach, stem cells are under research with a focus on reducing the burden of several kidney diseases. (biomedcentral.com)
Collectively, although scientists are increasingly aware of mitochondria's dynamic and transferable capabilities of mitochondria, the unclear mechanism and effectiveness of mitochondrial transfer/transplantation therapy limit its widespread application in diseases. (biomedcentral.com)

Can mesenchymal stem cells ameliorate testicular damage? (jsurgmed.com)

Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)

Furthermore, MDLS could promote teratoma formation and enhanced differentiation potential of P19 cells in vivo. (biomedcentral.com)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

1,2 Regarding the origin, they can be classified as embryonic stem cells (ESCs) and adult stem cells (ASCs). (bvsalud.org)
It is expressed in ESCs, embryonic germ (EG) cells and embryonic carcinoma (EC) cells and essential for early embryonic development [ 14 , 15 ]. (biomedcentral.com)

A median of 11 spot-forming cells and 10 spot-forming cells per 1 10 peripheral blood mononuclear cells in participants in the high dose and low dose groups, respectively, were observed at day 28. (regenerativemedicine.net)

Natural killer (NK) cell-based immunotherapy may overcome genetic mechanisms of resistance to T cell-based immunotherapy through antigen- and MHC-independent recognition of malignant cells. (elifesciences.org)
In addition to these basic issues concerning leukemia pathogenesis, we are devising new diagnostic procedures for detecting and monitoring leukemia patients based on molecular genetic abnormalities in the malignant cells. (stanford.edu)

Melton, D.A. "Stemness": Transcriptional profiling of embryonic and adult stem cells. (benthamscience.com)

Somatic cell nuclear transfer is a cloning method that can be used to create a cloned embryo for the use of its embryonic stem cells in stem cell therapy. (wikipedia.org)
Depending on the patient's medical condition, a refractory disease patient also requires an on-time selective option, such as less invasive cellular therapy options or curative organ transplantation that can function immediately after transplantation. (frontiersin.org)
Finally, one should consider cell choice, a critical aspect of any cell therapy. (biomedcentral.com)
Biological treatments (i.e., stem-cell therapy, bioengineering) have made great progress in the last years. (raaot.org.ar)
Preclinical studies suggest that cell therapy may be safe and effective in improving functional outcomes. (bmj.com)
Several recent clinical trials have reported safety and some improvement in outcomes following cell therapy administration in ischaemic stroke, which are reviewed. (bmj.com)
Cell therapy may provide a promising new treatment for stroke reducing stroke-related disability. (bmj.com)
Cell therapy is one approach to enhancing recovery after stroke. (bmj.com)
9 Clinical trials of cell therapy completed in the 2000s mostly treating small cohorts of patients with chronic stroke demonstrated adequate safety and a suggestion of efficacy with the use of cell therapy. (bmj.com)
13 These early clinical trials mostly focused on chronic subcortical strokes, but more recent trials are now investigating cell therapy for treatment of both cortical and subcortical infarcts. (bmj.com)
Will Stem Cell Therapy Help Cure Spinal Cord Injury? (regenerativemedicine.net)
A systematic survey of the scientific literature shows that stem cell therapy can have a statistically significant impact on animal models of spinal cord injury, and points the way for future studies. (regenerativemedicine.net)

OBJECTIVE: Oral squamous cell carcinoma (OSCC) is characterized by high recurrence and metastasis and places a heavy burden on societies worldwide. (bvsalud.org)

Treatment of syngeneic tumors resulted in CD8 and PD-L1-dependent tumor rejection or growth inhibition and a reduction in myeloid cells endogenously expressing high levels of PD-L1. (elifesciences.org)
T cell-based immunotherapy, such as immune checkpoint blockade or adoptive T cell transfer, is limited by the ability of T cells to detect major histocompatibility complex (MHC)-presented antigen by tumor cells. (elifesciences.org)

This is because stem cells are, basically, the core building blocks of all human cells - which is why they are most prominently a part of human physiology during gestation in the womb. (articlecity.com)
Stem cells are unspecialized cells of the human body. (articlecity.com)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
Stem cell technologies have opened up new avenues in the study of human biology and disease. (ehu.eus)
The invention provides an artificial antigen presenting cell (AAPC) comprising a eukaryotic cell expressing an antigen presenting complex comprising a human leukocyte antigen (HLA) molecule of a single type, at least one exogenous accessory molecule and at least one exogenous T cell-specific epitope. (patsnap.com)
The vaccine in this trial uses a weakened human common cold virus (adenovirus, which infects human cells readily but is incapable of causing disease) to deliver genetic material that codes for the SARS-CoV-2 spike protein to the cells. (regenerativemedicine.net)

Objective: the aim of this study was to conduct a literature review of the types of stem cells of dental origin and their applications in Dentistry. (bvsalud.org)
In animal models, delivery of several different types of stem cells reduce infarct size and improve functional outcomes. (bmj.com)

However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
Recently, there has been growing interest in the therapeutic potential of using a cell-based approach to treat vasodegenerative disorders. (biomedcentral.com)
AIM OF THE STUDY: To reveal the potential mechanisms of JQZF inhibiting B cell proliferation and activation in MRL/lpr mice. (bvsalud.org)

Many recent studies have demonstrated the therapeutic effects of mesenchymal stem cells (MSC) in different disease models. (jsurgmed.com)

High-profile campaigners like the late actor Christopher Reeve, himself a victim of sports-related spinal cord injury, have placed high hopes in stem cell transplantation. (regenerativemedicine.net)
Many studies have been performed that involve animal models of spinal cord injury (mostly rats and mice), but these are limited in scale by financial, practical, and ethical considerations. (regenerativemedicine.net)

This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)

The efficiency of transplanted stem cells reduces due to conditions like hypoxia, inflammation, nutrient deprivation, immunogenicity, extracellular matrix loss on delivery and mechanical stress. (benthamscience.com)

The purpose is to deliver chemotherapy, immunotherapy, and/or radiation to eliminate malignancy, prevent rejection of new stem cells, and create space for the new cells. (medscape.com)

Stem cells exist both in embryos and adult cells. (articlecity.com)

The ideal treatment option for terminally ill patients is organ transplantation. (frontiersin.org)

They therefore represent a more realistic cellular environment for modeling the cell biology and pathology of the nervous system. (ehu.eus)

We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)

Similarly, it opens a new path for the treatment and repair of damaged renal cells. (biomedcentral.com)

Anatomy15

Organisms3

Diseases25

Chemicals and Drugs20

Analytical, Diagnostic and Therapeutic Techniques and Equipment48

Psychiatry and Psychology1

Phenomena and Processes15

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science2

Named Groups3

Health Care10

Pluripotent19

Vitro17

Hematopoietic stem cell trans2

MSCs18

Progenitor cells7

Self-renewal11

Promote the differentiation1

Marrow Transplantation3

Tissue32

Allogeneic2

Proliferation and differentiation1

Process of differentiation1

Multipotent3

Regenerative9

Differentiate4

Umbilical1

Apoptosis6

Tissues8

Cancer16

Regeneration2

Bone5

IPSCs4

Methods15

Pathways3

Proliferative1

Adipose3

Genes6

Diseases3

Testicular1

Populations1

Vivo2

ESCs2

Peripheral blood1

Malignant cells2

Embryonic and adult1

Therapy12

Metastasis1

Tumor2

Human7

Types of stem cells2

Potential3

Therapeutic effects1

Spinal cord2

Mechanisms1

Reduces1

Immunotherapy1

Adult cells1

Terminally1

Biology1

Transcriptional1

Repair of damaged renal1