ScaffoldsProliferation and differentiationRegenerationChitosanInjectableStiffnessOsteogenesisMSCs2019BiofabricationCollagenElectrospunOsteoblastSoft biomaterialsNovel biomaterialsBioactiveOsteogenic differentiationStemMechanicalCharacterizationVitro and in vivoAngiogenicCartilage tissuePolymer networkCelluloseFibersVivoRegenerative medicineConstructsBiologicalBioengineeringViabilityTissuesNanocompositeBiocompatibleMimicEvaluationsMacrophageCellsCellBiopolymersMineralizationPeptideResembleFunctional
Scaffolds13
- Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
- Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
- These biomembranes or scaffolds constitute a support for osteoblastic adhesion and proliferation, but also microenvironment for stem cells' chemotactism and differentiation [ 1 ]. (hindawi.com)
- The Ideal candidate will have experience in design, fabrication, and testing of novel biomaterials, particularly biomimetic scaffolds and membranes for tissue engineering/wound healing applications. (nas.edu)
- Among different types of scaffolds, polymeric hydrogel scaffolds have received considerable attention because of their biocompatibility and structural similarity to native tissues. (mdpi.com)
- However, hydrogel scaffolds have several limitations, such as weak mechanical property and a lack of bioactive property. (mdpi.com)
- The scaffolds were prepared using collagen, elastin, and gelatin methacryloyl, and were crosslinked with Irgacure or Irgacure and Genipin. (rheolution.com)
- Hydrogel scaffolds show promise for use in periodontal tissue engineering. (opendentistryjournal.com)
- therefore, collagen hydrogel scaffolds may serve as a carrier of aqueous signaling molecules. (opendentistryjournal.com)
- Tissue engineering offers a great potential in regenerative dentistry and to this end, three dimensional (3D) bioprinting has been emerging nowadays to enable the incorporation of living cells into the biomaterials (such a mixture is referred as a bioink in the literature) to create scaffolds. (desktopmetal.com)
- Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
- Consequently, enhancing the adhesion of MSCs, augmenting the release of growth factors, and promoting angiogenic potential of biomaterial scaffolds after implantation are pivotal for successful bone regeneration. (frontiersin.org)
- To overcome these drawbacks, alginate (Alg)/poly-L-ornithine (PLO) PEC hydrogels are fabricated into microcapsules, fibers, and bulk scaffolds to explore their feasibility as drug and cell carriers. (desktopmetal.com)
Proliferation and differentiation2
- In tissue engineering strategies for periodontitis, cellular proliferation and differentiation should be facilitated in order to regenerate alveolar bone and functional periodontal attachment [ 1 - 3 ]. (opendentistryjournal.com)
- Accumulating evidence supports the view that the role of MSCs in regenerative medicine is mainly achieved by the paracrine pathway rather than direct proliferation and differentiation at the injured site. (biomedcentral.com)
Regeneration11
- Her research group is particularly interested in developing biomaterials and cell-based therapeutics to improve regeneration of various musculoskeletal tissues including bone, cartilage, tendon, blood vessels etc. (stanford.edu)
- Thus, the strategy of functionalization of nanofibers by nanoreservoirs of BMP-2 or BMP-7 showed a great efficiency for bone regeneration and increased the differentiation of MSCs (mesenchymal stem cells), accelerating the tissue regeneration in vivo [ 9 , 10 , 13 , 14 ]. (hindawi.com)
- It includes tissue-engineered products that rely on the combination of biomaterials, cells and inductive biomolecules to promote tissue and organ regeneration. (rheolution.com)
- These IPN biomaterials show favorable chemical, mechanical, and morphological properties and can potentially assist in the restoration of neurological function and neural tissue regeneration. (rheolution.com)
- When the hydrogels were used to transplant MSCs, the hydrogel's elasticity regulated bone regeneration, with optimal bone formation at 60 kPa. (nature.com)
- She was awarded with a postdoc fellow from the Ministry of Science at the Technical University of Catalonia in Biomaterials for bone tissue regeneration and since 2010 has been professor at this university. (ibecbarcelona.eu)
- However, when employing MSC transplantation to treat tissue defects, the research found that: less than one percent of MSCs could home and colonize in the tissue defect area, continuously promoting tissue regeneration, and the paracrine mechanism of MSCs plays a more important role than the replacement of damaged cells by differentiation [ 13 ]. (biomedcentral.com)
- Statins are lipid lowering drugs and are routinely administered in treatment of hyperlipidemia.1,2 In the last two decades, scientists have found that statins have other mechanisms that may be bene- ficial in bone regeneration.3 Lipophilic statins such as Simvastatin increase osteogenesis by enhancing differentiation of mesenchy- mal cells into osteoblasts, upregulating bone morphogenetic pro- tein-2 (BMP-2) and down regulating osteoblast apoptosis. (syksignaling.com)
- The aim was to accelerate tissue regeneration by adsorbing growth factors and/or seeding human mesenchymal stem cells on the biomaterial. (universityofgalway.ie)
- Her research is focused on developing biomaterials and harnessing their potential for tissue regeneration. (southampton.ac.uk)
- The effects of shock wave stimulation of mesenchymal stem cells on proliferation, migration, and differentiation in an injectable gelatin matrix for osteogenic regeneration. (uni-rostock.de)
Chitosan4
- Present feedstocks for biomaterials are limited to human tissue , which has strong resistance to and heavily hydrogenated collagen, gelatin, fibrin and chitosan. (blogmech.com)
- PEG-penetrated chitosan-alginate co-polysaccharide-based partially and fully cross-linked hydrogels as ECM mimic for tissue engineering applications. (docksci.com)
- In the current study, novel polyethylene glycol interpenetrated cross-linked hydrogel scaffold based on a co-polysaccharide (PIAC) synthesized from two marine heteropolysaccharides, alginate and chitosan, was designed. (docksci.com)
- In this study, a porous piezoelectric hydrogel bone scaffold was fabricated by incorporating polydopamine (PDA)-modified ceramic hydroxyapatite (PDA-hydroxyapatite, PHA) and PDA-modified barium titanate (PDA-BaTiO 3 , PBT) nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. (biomedcentral.com)
Injectable2
- W. Niu, T. C. Lim, A. Alshihri, R. Rajappa, L. S. Wang, M. Kurisawa, M. Spector, Platelet-derived growth factor stimulated migration of bone marrow mesenchymal stem cells into an injectable gelatin-hydroxyphenyl propionic acid matrix, Biomedicines 9, 203 (2021). (labby.jp)
- Here, by developing injectable, void-forming hydrogels that decouple pore formation from elasticity, we show that mesenchymal stem cell (MSC) osteogenesis in vitro , and cell deployment in vitro and in vivo , can be controlled by modifying, respectively, the hydrogel's elastic modulus or its chemistry. (nature.com)
Stiffness4
- With a stiffness suitable for softer tissue engineering applications, the IPN biomaterials resemble the native rat cortex. (rheolution.com)
- Furthermore, this differentiation was found to be influenced by the type of microsphere and the ability of the cells to migrate towards them, which was highly dependent on the stiffness of the bioink. (ibecbarcelona.eu)
- The proposed project aims to design a novel molecular hydrogel platform based on novel molecular materials [3-8] to create synthetic extracellular matrix with tunable stiffness and oxygen self-generation ability to modulate macrophage polarization. (findaphd.com)
- Ionically and Enzymatically Dual Cross-Linked Oxidized Alginate Gelatin Hydrogels with Tunable Stiffness and Degradation Behavior for Tissue Engineering. (uni-rostock.de)
Osteogenesis4
- Figure 2: Manipulating stem cell osteogenesis and proliferation by controlling the elasticity of the bulk phase of void-forming hydrogels. (nature.com)
- The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells. (nature.com)
- Simvastatin promotes osteogenesis by increasing viability and differentiation of osteoblasts. (syksignaling.com)
- The piezoelectric Cs/Gel/PHA/PBT hydrogels developed here with favorable immunomodulation, angiogenesis, and osteogenesis functions may be used as a substitute in periosteum injuries, thereby offering the novel strategy of applying piezoelectric stimulation in bone tissue engineering for the enhancement of combat effectiveness in grassroots troops. (biomedcentral.com)
MSCs1
- She developed a process to fabricate a stratified scaffold based on alginate hydrogel laden with human mesenchymal stem cells (MSCs) from the aspirate bone marrow. (universityofgalway.ie)
20191
Biofabrication1
- She has worked on designs and chemical modification of scaffold/hydrogel and assessment of them by in vitro and in vivo studies, and published in journals such as Nature Communications, Biomaterials, Biofabrication, and Advanced Healthcare Materials. (southampton.ac.uk)
Collagen10
- Collagen hydrogel scaffold exhibits bio-safe properties and facilitates periodontal wound healing. (opendentistryjournal.com)
- We evaluated whether periodontal wound healing is promoted by application of collagen hydrogel scaffold in combination with FGF2 in furcation defects in beagle dogs. (opendentistryjournal.com)
- Collagen hydrogel was fabricated from bovine type I collagen with an ascorbate-copper ion cross-linking system. (opendentistryjournal.com)
- Collagen hydrogel was mingled with FGF2 and injected into sponge-form collagen. (opendentistryjournal.com)
- Subsequently, FGF2 (50 µg)/collagen hydrogel scaffold and collagen hydrogel scaffold alone were implanted into class II furcation defects in dogs. (opendentistryjournal.com)
- FGF2-loaded collagen hydrogel scaffold possessed excellent biocompatibility and strongly promoted periodontal tissue engineering, including periodontal attachment re-organization. (opendentistryjournal.com)
- Recently, type I collagen hydrogel was prepared by an ascorbate-copper ion system [ 10 ] and its bio-safety was confirmed in animals [ 11 ]. (opendentistryjournal.com)
- In particular, cell ingrowth and subsequent self-assembly organization were frequently demonstrated on collagen hydrogel application. (opendentistryjournal.com)
- 13 ] revealed that implantation of a three-dimensional scaffold, composed of collagen hydrogel and sponge form, facilitated periodontal healing, including reconstruction of the periodontal attachment apparatus. (opendentistryjournal.com)
- Thus, we hypothesized that a combination of FGF2 and collagen hydrogel scaffold dramatically improved the biological effects for periodontal tissue engineering. (opendentistryjournal.com)
Electrospun7
- Expertise in tuning hydrogel or electrospun biomaterials for specific applications is valued. (nas.edu)
- Bischel, LL, et al: "Electrospun gelatin biopapers as substrate for in vitro bilayer models of blood-brain barrier tissue. (nas.edu)
- Electrospun biomaterials for bone tissue engineering. (dokumen.pub)
- Electrospun nanofiber-reinforced hydrogels. (dokumen.pub)
- Electrospun hydrogels with biological electrospray cells. (dokumen.pub)
- Electrospun hydrogels with antimicrobial activity. (dokumen.pub)
- Future applications of electrospun hydrogels. (dokumen.pub)
Osteoblast2
- Sim- vastatin induces osteoinduction by increasing osteoblast activ- ity and differentiation and inhibiting their apoptosis. (syksignaling.com)
- Through interactions such as hydrogen bonding between Go and the Rig-loaded GO-hydrogel beads, the bead-loaded microfluidic device supported MC3T3 proliferation and development as osteoblast without additional osteogenic differentiation supplements. (biomedcentral.com)
Soft biomaterials1
- Nanoindentation refers to a class of experimental techniques where a micrometric force probe is used to quantify the local mechanical properties of soft biomaterials and cells. (jove.com)
Novel biomaterials1
- Dr. Yang has extensive expertise in developing novel biomaterials and stem cell-based therapeutics for musculoskeletal tissue engineering, or engineering 3D in vitro cancer models for drug screening and mechanistic discovery. (stanford.edu)
Bioactive1
- Specifically, we are interrogating the effects of fiber alignment and the inclusion of bioactive adhesion factors gelatin and fibronectin on cell behavior. (davislab.org)
Osteogenic differentiation1
- We subsequently discuss how β-TCP can regulate osteogenic processes to aid bone repair/healing, namely osteogenic differentiation of mesenchymal stem cells, formation of blood vessels, release of angiogenic growth factors, and blood clot formation. (frontiersin.org)
Stem7
- Different cell lines and cell types have been cultured in NFC, including e.g. differentiation of human hepatic cells to functional organotypic cultures, and proliferation of human pluripotent stem cells. (wikipedia.org)
- For example, the seminal work by Engler and colleagues demonstrated that mesenchymal stem cell linage commitment is controlled by matrix elasticity, as studied on soft and stiff two-dimensional polyacrylamide (PAAm) hydrogels 7 . (jove.com)
- S. Tan, A. Yamashita, S. J. Gao, M. Kurisawa, Hyaluronic acid hydrogels with defined crosslink density for the efficient enrichment of breast cancer stem cells, Acta Biomater . (labby.jp)
- She is the Director and PI for Stem Cells and Biomaterials Engineering Laboratory at Stanford University, jointly supported by Departments of Orthopaedic Surgery and Bioengineering. (stanford.edu)
- These problems can be partially circumvented by using macroporous biomaterials that improve the survival of transplanted stem cells and provide molecular cues to direct cell phenotype. (nature.com)
- Khetan, S. & Burdick, J. A. Patterning network structure to spatially control cellular remodeling and stem cell fate within 3-dimensional hydrogels. (nature.com)
- Scaffold implantation therapy plays a significant role in repopulation and differentiation of stem cells and extracellular matrices when periodontal surgery is performed, resulting in a periodontal regenerative effect. (opendentistryjournal.com)
Mechanical6
- This approach has gained a central role in the fields of mechanobiology, biomaterials design and tissue engineering, to obtain a proper mechanical characterization of soft materials with a resolution comparable to the size of single cells (μm). (jove.com)
- From whole tissues to single cells, mechanical properties can inform about the pathophysiological state of the biomaterial under investigation 3 , 4 . (jove.com)
- Analysis techniques including mechanical characterization of materials, as well as assessment of biocompatibility and cellular differentiation via gene expression, immunohistochemistry, qPCR, are requested. (nas.edu)
- The mechanical properties of engineered tissues evolve with time since cells are able to degrade the initial biomaterial and produce ECM proteins. (rheolution.com)
- By tuning the mechanical properties of the hydrogels and the concentration of self-generated molecular oxygen, we aim to investigate the crosstalk between the hydrogel mechanics and molecular oxygen level on macrophage polarization and their respective molecular pathways. (findaphd.com)
- Alginate-based hydrogels show the same complex mechanical behavior as brain tissue. (uni-rostock.de)
Characterization1
- Figure 1: Fabrication and characterization of void-forming hydrogels. (nature.com)
Vitro and in vivo2
- Figure 3: Controlling cell deployment kinetics from void-forming hydrogels in vitro and in vivo . (nature.com)
- Cell and animal experiments were performed to characterize the immunomodulatory, angiogenic, and osteogenic capabilities of the piezoelectric hydrogel scaffold in vitro and in vivo. (biomedcentral.com)
Angiogenic1
- they not only effectively induced macrophage polarization to M2 phenotype but also promoted the migration, tube formation, and angiogenic differentiation of human umbilical vein endothelial cells (HUVECs) and facilitated the migration, osteo-differentiation, and extracellular matrix (ECM) mineralization of MC3T3-E1 cells. (biomedcentral.com)
Cartilage tissue1
- Computational study on electromechanics of electroactive hydrogels for cartilage-tissue repair. (uni-rostock.de)
Polymer network1
- This study introduces two novel smart polymer 3D-printable interpenetrating polymer network (IPN) hydrogel biomaterials for potential applications in traumatic brain injury (TBI). (rheolution.com)
Cellulose1
- Hydrogel from wood-based nanofibrillated cellulose (NFC) is used as a matrix for 3D cell culture, providing a three-dimensional environment that more closely resembles the conditions found in living tissue. (wikipedia.org)
Fibers2
- These fibers can be used to create a hydrogel, which is a type of material that is made up of a network of cross-linked polymer chains and is able to hold large amounts of water. (wikipedia.org)
- Also, most current cell-laden PEC hydrogels are limited to fibers and microcapsules with unfavorable dimensions and structures for practical implantations. (desktopmetal.com)
Vivo2
- As the natural extracellular matrix (ECM) is important in the survival, proliferation, differentiation and migration of the cells, hydrogels mimicking natural ECM structure are considered as potential approaches towards in vivo -like cell culturing. (wikipedia.org)
- The in vivo evaluations showed that these piezoelectric hydrogels with versatile capabilities significantly facilitated new bone formation in a rat large-sized cranial injury model. (biomedcentral.com)
Regenerative medicine1
- She has established expertise in the design of advanced biomaterials and biointerfaces for their application in nanomedicine, regenerative medicine and Lab-on-a-chip devices. (mcgill.ca)
Constructs2
- Multicomponent hydrogels for the formation of vascularized bone-like constructs in vitro. (findaphd.com)
- The hydrogels with pre-differentiated (into tenogenic, chondrogenic, and osteogenic lineages) or undifferentiated rabbit ADMSCs were 3D-bioprinted into zonal-specific constructs to mimic the structure of the tendon-to-bone interface. (desktopmetal.com)
Biological4
- Hydrogels generally consist of synthetic and naturally hydrated polymers, and exhibit physical and biological properties [ 4 ]. (opendentistryjournal.com)
- The biological evaluations confirmed the healthy being and 3D growth of fibroblasts on the porous networks of both the hydrogels. (docksci.com)
- The development of novel interface for improved interaction of biomaterials with biological environment is the overall objective of our research. (mcgill.ca)
- Supramolecular self-assembly to control structural and biological properties of multicomponent hydrogels. (findaphd.com)
Bioengineering1
- M. Nadra, W. Niu, M. Kurisawa , D. Rousson M. SpectorPlatelet-Rich Plasma Lysate-Incorporating Gelatin Hydrogel as a Scaffold for Bone Reconstruction, Bioengineering 9, 513 (2022). (labby.jp)
Viability2
- The hydrogel-based bioinks offered high cell viability and proliferative capability for rabbit ADMSCs. (desktopmetal.com)
- MC3T3 osteoblastic cells were cultured in a microchannel with Ris-loaded GO-hydrogel beads, and their proliferation, viability, attachment and spreading were assessed for a week. (biomedcentral.com)
Tissues2
- Engineered tissues have been developed using biomaterials and cells to mimic the properties of the damaged tissue with the ultimate purpose of repairing or replacing it. (rheolution.com)
- Cellularized hydrogels have been widely investigated for producing in vitro models of tissues such as skin, blood vessels, bone, etc. (rheolution.com)
Nanocomposite1
- Growth‐factor free multicomponent nanocomposite hydrogels that stimulate bone formation. (findaphd.com)
Biocompatible1
- 3D and 2D biocompatible hydrogels and sponges for molecular therapy and tissue engineering. (mcgill.ca)
Mimic1
- The present hydrogel can form an ECM mimic and can form a potent candidate for various tissue engineering applications. (docksci.com)
Evaluations1
- The physiochemical evaluations of both the hydrogels revealed the presence of alginate fraction and extensive -OH groups on the surface, sufficient water content and water holding capacity. (docksci.com)
Macrophage1
- The underlying molecular mechanism can be partly attributed to the immunomodulation of the Cs/Gel/PHA/PBT hydrogels as shown via transcriptome sequencing analysis, and the PI3K/Akt signaling axis plays an important role in regulating macrophage M2 polarization. (biomedcentral.com)
Cells5
- The protocol presents a complete workflow for soft material nanoindentation experiments, including hydrogels and cells. (jove.com)
- In this protocol, a step-by-step guide detailing the experimental procedure to acquire nanoindentation data on hydrogels and cells using a commercially available ferrule-top optical fiber sensing nanoindenter is presented. (jove.com)
- MTT assay on hydrogel extracts and direct contact assay showed the nontoxic effects of fibroblast cells upon contact with the hydrogel. (docksci.com)
- Live/dead assay using ethidium bromide/acridine orange cocktail on fibroblast cells grown on the hydrogels after 5 days of initial seeding displayed green nucleus revealing the non-apoptotic cells. (docksci.com)
- We further found that MC3T3 cells proliferated continuously in vitro in the microfluidic channel containing the GO-hydrogel system. (biomedcentral.com)
Cell11
- GrowDex is NFC hydrogel for 3D cell culture commercialized by UPM, Finland. (wikipedia.org)
- The nanocellulose hydrogel can be completely degraded by cellulase enzyme treatment while retaining the 3D cell structures. (wikipedia.org)
- NFC hydrogel in 3D cell culture offers a platform for various biomedical applications. (wikipedia.org)
- Additionally, 3D cell culture using wood-based nanocellulose hydrogel can be used for tissue engineering. (wikipedia.org)
- A clear advantage of cell sheet technology for therapeutic applications is the absence of biomaterials, which reduces the risk of immune rejection that could arise from xenobiotic or non-autologous materials, and that no suture is needed to ligate the construct to the injured heart. (encyclopedia.pub)
- Interestingly, medical materials should allow cell adhesion, growth and differentiation. (blogmech.com)
- In addition, we are engineering cell-laden biomaterials to be 3D printed using 3D models generated from patient CT. (davislab.org)
- We aim at building a deeper understanding of cell-biomaterial interactions in order improve our ability to predict and control the host response to new material. (mcgill.ca)
- Cell-cell and cell-biomaterial interactions. (mcgill.ca)
- The results showed that the addition of microspheres generally improved the rheological properties of the ink, favoured cell proliferation and positively affected osteogenic cell differentiation. (ibecbarcelona.eu)
- Polyelectrolyte complex (PEC) hydrogels are advantageous as therapeutic agent and cell carriers. (desktopmetal.com)
Biopolymers1
- Various biomaterials are available to produce skin substitutes, including natural biopolymers and synthetic polymers. (medichub.ro)
Mineralization1
- De Novo design of functional coassembling organic-inorganic hydrogels for hierarchical mineralization and neovascularization. (findaphd.com)
Peptide1
- The biomaterials exhibited thermal stability, amorphous nature, and maintained the peptide secondary structure. (rheolution.com)
Resemble1
- NFC fiber network structure and dimensions in hydrogel resemble human ECM. (wikipedia.org)
Functional1
- PIAC-P hydrogels were superior to certain aspects due to the availability of free functional groups than PIAC-F where most of these groups were utilized for cross-linking. (docksci.com)