Differentiation functional cartilage on scaffolds tissue engineering. Medical search. Frequent questions

ACS Biomaterials Science & Engineering. (chop.edu)
The scaffolds have several empirical advantages over man-made biomaterials (synthetic or biological) the most important of which is their content of native growth factors (e.g. cartilage-derived morphogenetic proteins or CDMPs) and native extracellular matrix molecules that provide crucial signals to regulate the differentiation of the MSCs into functional hyaline cartilage. (rochester.edu)
Regenerative techniques aim to create a more favorable environment for cartilage and bone regeneration by using various biomaterials, growth factors, and cells. (surgeonsync.com)
These approaches involve the use of biomaterials, growth factors, and cells to create functional and durable cartilage and bone tissue. (surgeonsync.com)
Biomaterials play a crucial role in tissue engineering by providing a scaffold for cell attachment, proliferation, and differentiation. (surgeonsync.com)
To date, numerous stem cells and biomaterials have been explored for a variety of tissue and organ regeneration. (routledge.com)
She is the Director and PI for Stem Cells and Biomaterials Engineering Laboratory at Stanford University, jointly supported by Departments of Orthopaedic Surgery and Bioengineering. (stanford.edu)
Dr. Yang has extensive expertise in developing novel biomaterials and stem cell-based therapeutics for musculoskeletal tissue engineering, or engineering 3D in vitro cancer models for drug screening and mechanistic discovery. (stanford.edu)
Her research group is particularly interested in developing biomaterials and cell-based therapeutics to improve regeneration of various musculoskeletal tissues including bone, cartilage, tendon, blood vessels etc. (stanford.edu)
We invent biomaterials and engineering tools to elucidate and modulate biology, and also use biology to inform materials and engineering design. (stanford.edu)
TE strategies typically incorporate cells, biomaterials and signals (e.g. growth factors), with the goal of developing a construct that once implanted will promote tissue regeneration. (nature.com)
Tissue Engineering - Part A is a journal indexed in SJR in Biochemistry and Biomaterials with an H index of 128. (countryofpapers.com)
As a biomaterials scientist, his research focuses on developing biomaterials for implantable medical devices and tissue engineering templates and, specifically, on experimental and clinical work aimed at understanding biocompatibility phenomena [2], [3]. (embs.org)
Regenerative medicine is being driven by multiple factors: a shortage of organs available for transplantation, advances in biomaterials, a greater knowledge of the biological sciences, and a desire to produce functional replacement tissues. (embs.org)
Therefore, researchers have begun exploring combined therapies with biomaterials that promote cell growth and differentiation while localizing cells in the injured area. (karger.com)
She was awarded with a postdoc fellow from the Ministry of Science at the Technical University of Catalonia in Biomaterials for bone tissue regeneration and since 2010 has been professor at this university. (ibecbarcelona.eu)
Cruz Perez B, Tang J, Morris HJ, Palko JR, Pan X, Hart RT, Liu J. Scaffold download drawing made easy dragons fantasy unleash your creative beast as you conjure up biomaterials presence giving, hiringContact, and siehe coverage. (it-dresden.net)
Current research in tissue engineering is geared towards elucidating the appropriate compositional elements (biomaterials, biomolecules and cell sources) as well as methods of assembly. (biomedcentral.com)
By utilizing various strategies, such as stem cell therapy, tissue engineering, and biomaterials, regenerative medicine offers promising solutions for addressing a wide range of medical conditions that currently have limited treatment options. (regbiomed.com)
This includes bioactive scaffolds, bioresorbable implants, injectable hydrogels, "organ-on-a-chip" organ models, and self-healing biomaterials. (boydbiomedical.com)
Bioactive scaffolds designed for bone repair and regeneration have emerged as one of the fastest-growing applications in the field of biomaterials. (boydbiomedical.com)
Choosing the correct biomaterials for bone scaffolds is crucial to ensure compatibility and effectiveness. (boydbiomedical.com)
Hydrogel-based biomaterials have emerged as promising candidates for scaffold production in artificial cartilage construction, owing to their water-rich composition, biocompatibility, and tunable properties. (bvsalud.org)
Future studies should explore pre-implant seeding of polymer scaffolds, more advanced polymer fabrication methods able to more closely approximate native tissue structure and function, and other techniques to control and balance the degradation of biomaterials and new tissue formation by modulation of the host immune response. (bvsalud.org)

One promising regenerative technique is autologous chondrocyte implantation (ACI), which involves harvesting healthy cartilage cells from the patient, expanding them in the laboratory, and then implanting them into the defect. (surgeonsync.com)
Bone grafting is a common procedure in orthopaedic surgery and the implantation of autologous bone grafts supplying osteoinductive growth factors, osteogenic cells, and a structural scaffold, has become the gold standard for the surgical treatment of bone defects caused by trauma, tumor, infection or congenital abnormalities. (biomedcentral.com)
Because of their capacity for ex vivo proliferation and differentiation they provide a good source of osteoprogenitor cells within custom-shaped scaffolds for implantable autologous bone tissue thus allowing the generation of a large transplantable cell population from a small biopsy [ 5 - 11 ]. (biomedcentral.com)
Nasal chondrocyte-based engineered autologous cartilage tissue for repair of articular cartilage defects: an observational first-in-human trial. (unibas.ch)
Different types of autologous platelet concentrate (PCs) are used in bone and cartilage regeneration. (spandidos-publications.com)
Tissue engineering and function reconstruction through host remodeling and autologous cell recruitment effectively was shown to overcome the aforementioned limitations and represent a fundamental shift from cell-based approaches ( 6-8 ). (spandidos-publications.com)
Consequently, different types of platelet concentrates (PCs) should also be regarded as alternative sources of autologous growth factors for cartilage regeneration ( 11 ). (spandidos-publications.com)

Dynamic Compressive Loading Improves Cartilage Repair in an in vitro Model of Microfracture: Comparison of Two Mechanical Loading Regimens on Simulated Microfracture Based on Fibrin Gel Scaffolds Encapsulating Connective Tissue Progenitor Cells. (chop.edu)
Engineering in vitro vascularized bone models for drug screening and predictive toxicology. (chop.edu)
To evaluate the effects of applied cytomechanical forces towards cellular differentiation distinct parameters were measured (morphology, antigen and antigen expression) after a total cultivation period of 21 days in vitro. (biomedcentral.com)
During the last years tissue engineering based treatment concepts and cell therapeutics showed promising results in vitro . (biomedcentral.com)
Our approach is to develop in vivo and in vitro models of musculoskeletal injury and clinically-translatable outcome measures to assess the efficacy of regenerative and tissue engineering solutions. (rochester.edu)
Pathways of human organogenesis - how cells self-assemble into complex tissues in vitro and in vivo . (iqscloud.net)
Superior MCL-PHA/PCL scaffold performance has been demonstrated compared to MCL-PHA through experimental comparisons of a) morphological data using scanning electron microscopy and b) contact angle measurements attesting to improved CPC adhesion, c) in vitro confocal microscopy showing increased SC proliferative capacity, d) mechanical testing that elicited good overall responses. (westminster.ac.uk)
In vitro MRI results justify the increased seeding density, increased in vitro MRI signal, and improved MRI visibility in vivo, in the double-layered compared to the single-layered scaffolds. (westminster.ac.uk)
It is a highly biocompatible hydrogel whose physical properties can potentially be tailored to direct 3D cell growth and differentiation both in vitro and in vivo 10 , 11 , 12 . (nature.com)
This is accomplished through the integration of natural or synthetic scaffolds, cells, and biologically active molecules in concert with mechanical cues in in vitro preparations with subsequent implantation in vivo. (embs.org)
The cells can be employed alone or in conjunction with unique biologically-compatible scaffold structures to generate differentiated tissues and structures, both in vitro and in vivo. (epo.org)
In some cases, an increase in oligodendrocyte differentiation alongside neurons is further highlighted for functional improvements where the biomaterial was then tested for increased myelination both in vitro and in vivo. (karger.com)
Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)

Journal of Tissue Engineering and Regenerative Medicine. (chop.edu)
This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)
With advancements in surgical techniques, tissue engineering, and regenerative medicine, osteochondral defect repair has become a beacon of progress in the medical field. (surgeonsync.com)
Tissue engineering and regenerative medicine have revolutionized the field of osteochondral defect repair by providing innovative solutions to overcome the limitations of traditional surgical techniques. (surgeonsync.com)
Medical researchers increasingly regard tissue engineering and regenerative medicine as potential game changers when it comes to repairing damage from disease or injury and restoring function. (embs.org)
To understand the progress made and challenges ahead for this combined field, IEEE Pulse sought out two experts: David F. Williams, former global president (2012-2015) of the Tissue Engineering and Regenerative Medicine International Society, and Roderic I. Pettigrew, founding director of the National Institute of Biomedical Imaging and Bioengineering (NIBIB) at the National Institutes of Health (NIH) [1]. (embs.org)
How would you describe tissue engineering and regenerative medicine today? (embs.org)
Tissue engineering and regenerative medicine constitute a promising new field of medical research with the goal of generating or repairing human tissues, which in the coming years could transform clinical medicine. (embs.org)
The expanded concept of regenerative medicine includes tissue engineering but also incorporates research on the regeneration of tissue directly in vivo, where the body uses its own systems to repair, replace, or regenerate function in damaged or diseased tissue with the help of exogenous cells, scaffolds, or biological factors. (embs.org)
Their activities led to the creation of the Tissue Engineering Society, which evolved to include international efforts in tissue engineering and in 2005 became the Tissue Engineering and Regenerative Medicine International Society. (embs.org)
Since that time, the terms tissue engineering and regenerative medicine , which have historical and nuanced distinctions, have become largely interchangeable among the broader scientific community. (embs.org)
In: Encyclopedia of Tissue Engineering and Regenerative Medicine, 1. (unibas.ch)
Recent advances in regenerative medicine place us in a unique position to improve the quality of engineered tissue. (biomedcentral.com)
Combining biomimetic regenerative medicine strategies will allow us to improve tissue-engineered auricular cartilage with respect to biochemical composition and functionality, as well as microstructural organization and overall shape. (biomedcentral.com)
Due to recent advances in regenerative medicine and additive manufacturing we are entering into an age where we have the potential to replace 'like with like', by improving the quality of engineered tissue with respect to biochemical composition and functionality, as well as microstructural organization and overall shape. (biomedcentral.com)
We will use the lessons learnt from auricular cartilage tissue engineering to illustrate how combining additive manufacturing and regenerative medicine for tissue-engineering purposes can be used to create functional and durable tissue with potential to shift the paradigm in reconstructive surgery. (biomedcentral.com)

There are three types of cartilage in the human body: fibrocartilage, hyaline cartilage and elastic cartilage. (wikipedia.org)
Fibrocartilage is most often found in the intervertebral discs, elastic cartilage is found in the external ear and hyaline cartilage is found on many joint surfaces in the body. (wikipedia.org)
Replacement of hyaline cartilage (articular cartilage) is the most common application of synthetic cartilage. (wikipedia.org)
The repaired tissue is often fibrocartilage, which is structurally inferior to native hyaline cartilage. (surgeonsync.com)
Additionally, the repaired tissue may still not fully resemble native hyaline cartilage. (surgeonsync.com)
However, clinical efficacy of these treatments is not conclusive, as these treatments do not consistently regenerate hyaline cartilage. (biomedcentral.com)
The particular nature of hyaline articular cartilage, including the avascular nature and the low number of chondrocytes and stem cells in the surrounding cartilage lesions, results in limited potential to reconstruct osteochondral defects through a self-healing process ( 1 , 2 ). (spandidos-publications.com)

The field of tissue engineering is constantly evolving as it aims to develop bioengineered and functional tissues and organs for repair or replacement. (mdpi.com)
Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
Tissue engineering (TE) is a promising strategy for replacing, repairing or regenerating damaged tissues and organs. (nature.com)
You are ALIVE because you continuously renew (rejuvenate) various tissues/organs. (case.edu)
Broadly speaking, both terms refer to generating tissues, components of organs, and whole organs to repair or replace diseased, injured, or malfunctioning tissues. (embs.org)
Transplanting organs, tissues or cells from one person to another. (mayo.edu)
A disease that causes tissues or organs to deteriorate in structure or function over time. (mayo.edu)
Cells Tissues Organs (2016) 202 (1-2): 85-101. (karger.com)
Engineering Small-Scale and Scaffold-Based Bone Organs via Endochondral Ossification Using Adult Progenitor Cells. (unibas.ch)
Regenerative biomedicine holds tremendous potential for revolutionizing the field of medicine by harnessing the body's innate regenerative capabilities to restore damaged tissues and organs. (regbiomed.com)
Furthermore, we will examine various strategies employed in tissue engineering and biomaterial development to create functional replacements for damaged organs. (regbiomed.com)
By manipulating and modifying cells, researchers aim to harness their inherent ability to regenerate damaged tissues and organs. (regbiomed.com)
23.1 Specialized Tissues in Plants - 23.1 Specialized Tissues in Plants Essential Question What are plant tissues and organs? (powershow.com)

Artificial cartilage is a synthetic material made of hydrogels or polymers that aims to mimic the functional properties of natural cartilage in the human body. (wikipedia.org)
Efficacy of thermoresponsive, photocrosslinkable hydrogels derived from decellularized tendon and cartilage extracellular matrix for cartilage tissue engineering. (chop.edu)
We next explored if spatially modulating the stiffness of 3D bioprinted hydrogels could be used to direct MSC fate inside printed tissues. (nature.com)
8. Carroll, S.F., Buckley, C.T., Kelly, D.J. Measuring and modelling oxygen transport and consumption in 3D hydrogels containing chondrocytes and stem cells of different tissue origins. (buckleylab.eu)
Aizawa, Y., N.D. Leipzig, T. Zahira, M.S. Shoichet (2008) The effect of immobilized platelet derived growth factor AA on neural stem/progenitor cell differentiation on cell-adhesive hydrogels. (karger.com)
Aurand, E.R., K.J. Lampe, K.B. Bjugstad (2012) Defining and designing polymers and hydrogels for neural tissue engineering. (karger.com)
Nevertheless, conventional hydrogels typically lack the anisotropic structure inherent to natural cartilage, impeding their clinical and preclinical applications. (bvsalud.org)
Recent advancements in tissue engineering (TE) have introduced magnetically responsive hydrogels, a type of intelligent hydrogel that can be remotely controlled using an external magnetic field. (bvsalud.org)
In this review, we first explore conventional techniques employed for cartilage repair and subsequently delve into recent breakthroughs in the application and utilization of magnetic hydrogels across various aspects of articular cartilage TE. (bvsalud.org)

In the last two decades, the ECFCs from various vascular disease patients have been widely used to study the diseases' pathophysiology ex vivo and develop cell-based therapeutic approaches, including vascular regenerative therapy, tissue engineering, and gene therapy. (bvsalud.org)
In den letzten zwanzig Jahren wurden ECFCs von Patienten mit verschiedenen vaskulären Erkrankungen ex vivo verwendet, um die Pathophysiologie von Krankheiten zu studieren und Zell-basierte therapeutische Ansätze wie vaskuläre regenerative Therapie, tissue engineering, oder Gentherapie zu entwickeln. (bvsalud.org)
To date, however, stem cell-based tissue engineering remains far from clinical utility due in part to challenges associated with control of cell differentiation in vivo. (rochester.edu)
The challenge for existing stem cell-based techniques is that current therapies lack controlled environments that are crucial for regulating stem cell engraftment and differentiation in vivo , because stem cells are rather sensitive to even minute changes in their environment. (routledge.com)
Real-time, non-invasive modalities to track cells, and/or monitor tissue-function repair and integration process in vivo . (iqscloud.net)
Seeded scaffolds were implanted and studied in the post-mortem murine heart in situ, and in two additional C57BL/6 mice in vivo (using single-layered and double-layered scaffolds) and imaged immediately after and at 7 days post-implantation. (westminster.ac.uk)
One of the potential limitations associated with the use of alginate for in vivo tissue regeneration is that it is in general non-degradable by mammals, as they lack the enzymes needed to break down the polymer chains 21 . (nature.com)
Among these, adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cell isolated from adipose tissue, which has the advantages of abundant storage in vivo , easy acquisition, and expansion [ 13 - 15 ]. (hindawi.com)
An ex vivo study showed that IGF-1 in fetal bovine serum was responsible for maintaining articular cartilage proteoglycan synthesis. (biomedcentral.com)
In pursuit of a suitable scaffold material for cardiac valve tissue engineering applications, an acellular, electrospun, biodegradable polyester carbonate urethane urea (PECUU) scaffold was evaluated as a pulmonary valve leaflet replacement in vivo. (bvsalud.org)
The PECUU scaffolds had histologic evidence of scaffold degradation and an accumulation of pro-inflammatory/M1 and anti-inflammatory/M2 macrophages over time in vivo. (bvsalud.org)
2015) Functional genetic targeting of embryonic kidney progenitor cells ex vivo. (finmedtechnet.fi)

To address these limitations, we recently developed novel porous scaffolds derived from freeze-dried cartilage allograft tissue and demonstrated that these scaffolds can be efficiently seeded with stromal mesenchymal stem cells (MSCs) for cartilage tissue engineering. (rochester.edu)
For the quantification of cellular differentiation at the molecular level, osteogenic differentiation of MSCs is controlled by the interaction of hormones and transcription factors: runt-related transcription factor-2 (RUNX2) effectuates the expression of bone-specific genes, e.g. osterix (OSX), collagen type 1 alpha-1 (COL1A1), osteocalcin (OC), and bone sialoprotein (BSP) by binding to the promoters of these genes. (biomedcentral.com)
With the in-depth researches of MSCs, an increasing number of studies have indicated that the therapeutic effects of MSCs can be attributed not only to their differentiation capacity but also to their paracrine action [ 21 ]. (hindawi.com)
Besides stimulating ECM production, IGF-1 can stimulate the proliferation and chondrogenic differentiation of mesenchymal stem cells (MSCs) [ 7 ]. (biomedcentral.com)
When cartilage is damaged, MSCs derived from synovial fluid can partially move to the injured site and differentiate into chondrocytes to repair the defect, and IGF-1 induces chondrogenic differentiation [ 11 , 12 , 13 ]. (biomedcentral.com)
Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
However, MSCs have gained considerable attention for tissue regeneration owing to their differentiation ability with immunomodulatory effects. (techscience.com)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
These osteoconductive three dimensional constructs seeded with MSCs are highly porous, biodegradable and biomechanically stable scaffolds which do not evoke an immunogenic host cell response. (openorthopaedicsjournal.com)
Research has shown the importance of growth factors in guiding and modulating the differentiation of MSCs in order to obtain the required cell type. (openorthopaedicsjournal.com)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

Despite the abundance of surgical treatment options, current standards of care for cartilage defects repair have many complications that limit long-term benefits. (rochester.edu)
Osteochondral defects, also known as cartilage lesions, occur when there is damage to both the cartilage and underlying bone in a joint. (surgeonsync.com)
These conservative strategies can alleviate pain symptoms, but they cannot terminate the progression of cartilage deterioration and repair cartilage defects. (biomedcentral.com)
This method aims to regenerate rather than repair skeletal tissue defects. (openorthopaedicsjournal.com)
Regenerative Potential of Tissue-Engineered Nasal Chondrocytes in Goat Articular Cartilage Defects. (unibas.ch)
14.Steadman, J. R., Rodkey, W. G., Briggs, K. K. & Rodrigo, J. J. [The microfracture technic in the management of complete cartilage defects in the knee joint]. (howhelp.org)
Thereafter, these bioactive scaffolds were implanted into osteochondral defects surgically created in rabbits to assess their effects on tissue repair using micro‑CT scanning, histological observations and the evaluation scoring method for cartilage repair established by the International Cartilage Repair Society (ICRS). (spandidos-publications.com)
At 16 weeks after implantation surgery, full‑thickness osteochondral defects with a diameter of 5 mm and a depth of 4 mm were well‑filled with newly regenerated tissues, exhibiting the simultaneous regeneration of avascular articular cartilage and well‑vascularized subchondral bone, as proven through macroscopic and microscopic observations in PRF‑treated groups compared with that in the untreated group. (spandidos-publications.com)
The application of L‑PRF and F‑PRF for osteochondral defects in rabbits contributed to massive host remodeling and reconstruction of osteochondral tissues, thus offering a prospective bioactive scaffold for the simultaneous reconstruction of articular cartilage and subchondral bone tissue. (spandidos-publications.com)
Articular cartilage defects afflict millions of individuals worldwide, presenting a significant challenge due to the tissue's limited self-repair capability and anisotropic nature. (bvsalud.org)
2016) Bone healing in rabbit calvarial critical-sized defects filled with stem cells and growth factors combined with granular or solid scaffolds. (finmedtechnet.fi)

This has led to development of alternative tissue engineering approaches to enhance cartilage regeneration through combinations of cells (including stem cells) and three-dimensional biomaterial scaffolds. (rochester.edu)
The applications of biomaterial scaffolds for bone repair are wide-ranging. (boydbiomedical.com)
One of the critical advantages of biomaterial scaffolds for bone repair is their ability to guide and stimulate cell proliferation and differentiation. (boydbiomedical.com)
With advancements in materials science and fabrication techniques, these biomaterial scaffolds hold great promise for addressing the increasing prevalence of bone-related disorders and an increase in orthopedic interventions as the population ages. (boydbiomedical.com)

Micro- and nanotechnology hold great potential to fabricate biomimetic spatiotemporally controlled scaffolds as well as control stem cell behavior and fate by micro- and nanoscale cues. (routledge.com)
This program supports basic and translational research in the repair, regeneration, and restoration of function of injured and/or diseased musculoskeletal tissues using stem cell biology and engineering-based approaches. (iqscloud.net)
Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues. (nature.com)
Marrow-derived stem cell motility in 3D synthetic scaffold is governed by geometry along with adhesivity and stiffness. (nature.com)
Such a stem cell divides to produce like stem cells, some of which enter into a pathway of development and differentiation resulting in an end-phenotype which produces highly specialized molecules and/or functions and then, after a time, expires. (case.edu)
Center for Regenerative Biotherapeutics protocols have been developed to generate adult stem cell-derived bonelike, nerve-like and heart-like tissues. (mayo.edu)
Stem cell therapy is an exciting and upcoming branch of tissue engineering with application in the field of orthopaedics. (openorthopaedicsjournal.com)
Nevertheless, stem cell based tissue engineering in orthopaedics shows a promising future. (openorthopaedicsjournal.com)
2017-Present Istinye University Stem Cell and Tissue Engineering RD Manager. (livhospital.com)
Here we investigate the ability of resident skeletal stem-cell (SSC) populations to regenerate cartilage in relation to age, a possible contributor to the development of osteoarthritis5,6,7. (howhelp.org)
These data indicate that following MF, a resident stem-cell population can be induced to generate cartilage for treatment of localized chondral disease in OA. (howhelp.org)
Kuismanen K, Juntunen M, Narra Girish N, Tuominen H, Huhtala H, Nieminen K, Hyttinen J, Miettinen S. (2018) Functional Outcome of Human Adipose Stem Cell Injections in Rat Anal Sphincter Acute Injury Model. (finmedtechnet.fi)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)

My research focuses primarily on tissue engineering of load bearing connective tissues, including ligament and tendon, cartilage, and bone. (rochester.edu)
The MSC gives rise to bone-forming cells, cartilage-forming cells and cells of tendon, ligament fat, and dermis, as well as various connective tissues including the stroma of marrow. (case.edu)

In this study, we verified that exosomes derived from osteogenically differentiated ADSCs can promote osteogenic differentiation of ADSCs. (hindawi.com)
Moreover, we found that these differentially expressed exosomal miRNAs connect osteogenic differentiation to processes such as axon guidance, MAPK signaling, and Wnt signaling. (hindawi.com)
This study confirms that alterations in the expression of exosomal miRNAs can promote osteogenic differentiation of ADSCs, which also provides the foundation for further research on the regulatory functions of exosomal miRNAs in the context of ADSC osteogenesis. (hindawi.com)

By incorporating bioactive molecules and growth factors, these scaffolds can stimulate cell proliferation and differentiation, accelerate the healing process, and enhance bone regeneration. (boydbiomedical.com)
Platelet-rich fibrin (PRF), commonly known as a second-generation PC, was shown to have a high capacity to improve wound healing and tissue repair owing to the gradual release of growth factors during its slow degradation along with its intrinsic fibrin scaffolding, which offers a unique three-dimensional (3-D) microstructure for promoting proliferation and differentiation of recruited cells ( 12 , 13 ). (spandidos-publications.com)

Medium-chain length Polyhydroxyalkanoates (MCL-PHAs) have demonstrated exceptional properties for cardiac tissue engineering (CTE) applications. (westminster.ac.uk)
Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
The myocardial nicheplays a critical role in directing the function and fate of cardiomyocytes;therefore, engineering a biomimetic scaffold holds excellent promise.We produced an electroconductive cardiac patch of bacterial nanocellulose(BC) with polypyrrole nanoparticles (Ppy NPs) to mimic the naturalmyocardial microenvironment. (ibecbarcelona.eu)
Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
Our results highlight the remarkablepotential use of BC-Ppy scaffolds as a cardiac patch in tissue regenerativetherapies. (ibecbarcelona.eu)
However, regenerative biomedicine offers the possibility of repairing and restoring damaged cardiac tissue through innovative approaches like cellular reprogramming or transplantation of engineered heart tissues. (regbiomed.com)
To illustrate the power of cellular reprogramming, consider the case study of Jane, a 65-year-old woman suffering from heart failure due to damaged cardiac tissue. (regbiomed.com)
These iPSCs were then induced to differentiate into functional cardiac muscle cells known as cardiomyocytes. (regbiomed.com)
Once transplanted back into Jane's heart, these regenerated cardiomyocytes successfully integrated with existing tissue, leading to significant improvement in cardiac function. (regbiomed.com)

We developed a biomimetic nano-matrix with great promise to serve as a tissue engineering scaffold. (jove.com)
Newer techniques using tissue engineering to regenerate musculoskeletal tissue by using biomimetic materials are now being studied. (openorthopaedicsjournal.com)

Collagen: Collagen is a structural protein present in the extracellular matrix of cartilage. (wikipedia.org)
They can be synthetic or natural, and their properties can be tailored to mimic the native extracellular matrix of cartilage and bone. (surgeonsync.com)

Polymer characterization confirmed the chemical structure and composition of the synthesized scaffolds, while thermal, wettability, and mechanical properties were also investigated and compared in neat and porous counterparts. (westminster.ac.uk)
In this study, porous scaffold materials based on polyvinyl alcohol (PVA) and gelatin (Gel) were successfully fabricated and characterized. (mdpi.com)

Articular cartilage repair is a critical issue in osteoarthritis (OA) treatment. (biomedcentral.com)
The insulin-like growth factor (IGF) signaling pathway has been implicated in articular cartilage repair. (biomedcentral.com)

Tissue engineering principles include the use of cells, growth factors, and synthetic scaffolds in order to do this. (wikipedia.org)
Cells: Chondrocytes are an obvious choice to use in the regeneration of cartilage due to their ability to secrete collagen and other ECM components necessary for the functional properties of cartilage. (wikipedia.org)
Mesenchymal stem cells can also be used to create chondrocytes and make cartilage regeneration possible. (wikipedia.org)
Anatomical region-specific enhancement of 3-dimensional chondrogenic differentiation of human mesenchymal stem cells by soluble meniscus extracellular matrix. (chop.edu)
This clot contains cells that can differentiate into cartilage-like tissue, filling the defect. (surgeonsync.com)
A limitation of current TE strategies is their relatively poor spatial control of the distribution of cells, matrix components and bioactive cues within the engineered construct 1 . (nature.com)
Within the body, cells of the skin, gut, and blood, among others come to maturation, function for a time and then expire to be replaced by a continuous stream of cells which renew the tissue. (case.edu)
In every tissue in which this rejuvenation process occurs, a source exists which gives rise to these differentiated cells. (case.edu)
Following isolation and by mitotically expanding their numbers in culture, we can drive these cells down specific and different developmental pathways with emphasis on cartilage and bone. (case.edu)
Recently, a number of studies have confirmed that ADSCs possess the ability to differentiate into adipocytes, osteoblasts, and chondrocytes [ 16 - 18 ], suggesting that a broader source of stem cells is available for application in tissue engineering. (hindawi.com)
Thus, chondrocyte metabolism in the adjacent cartilage is relatively low, and these cells cannot easily migrate to the damaged site [ 2 ]. (biomedcentral.com)
Transplanting tissues or cells from one area of a person's own body to another. (mayo.edu)
Using specific types of stem cells to repair damaged tissues and treat disease. (mayo.edu)
The process of removing all the cells from a donor organ, such as a heart or lung, leaving behind just a tissue scaffold. (mayo.edu)
The presently disclosed subject matter generally relates to methods and systems for facilitating the growth and differentiation of adipose-derived stem cells for laboratory and therapeutic applications. (epo.org)
The presently disclosed subject matter further relates to methods of forming and using improved tissue engineered scaffolds that can be used as substrates to facilitate the growth and differentiation of cells. (epo.org)
The aim of this study is to evaluate the feasibility, safety, and efficacy of cell therapy based on mesenchymal stromal cells derived from adipose tissue intramuscular administration to patients with type 2 diabetes mellitus with critical limb ischemia and without possibility of revascularization. (unav.edu)
However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
This article reviews recent advancements in biomaterial strategies for the differentiation of neural stem cells into oligodendrocytes, and presents new data indicating appropriate properties for oligodendrocyte precursor cell growth. (karger.com)
Amidst this, tissue engineering using stem cells has emerged as a hot topic of research and is a promising alternative to the traditional techniques of bone grafting. (openorthopaedicsjournal.com)
Fat-Derived Stromal Vascular Fraction Cells Enhance the Bone-Forming Capacity of Devitalized Engineered Hypertrophic Cartilage Matrix. (unibas.ch)
We use auricular cartilage as an exemplar to illustrate how the use of tissue-specific adult stem cells, assembly through additive manufacturing and improved understanding of postnatal tissue maturation will allow us to more accurately replicate native tissue anisotropy. (biomedcentral.com)
The discussion will delve into key techniques used within this field, including stem cells derived from different sources (such as embryonic stem cells, induced pluripotent stem cells) and their applications in tissue regeneration. (regbiomed.com)
Incorporating bioactive molecules and growth factors within the scaffold provides a favorable microenvironment that promotes the recruitment and attachment of bone-forming cells, such as osteoblasts. (boydbiomedical.com)
Tissues Ch 4 - Tissues Ch 4 Tissues Groups of cells similar in structure and function The four types of tissues Epithelial Connective Muscle Nerve Epithelial Tissue 1. (powershow.com)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
All tissues originate from stem cells. (bvsalud.org)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)
These intradermal epithelial structures, such as sebaceous glands, sweat glands, and hair follicles, are lined with epithelial cells with the potential for division and differentiation. (medscape.com)

Clinical Applications of Bone Tissue Engineering in Orthopaedic Trauma. (chop.edu)
These bioactive cues can trigger specific cellular responses, including the production of extracellular matrix and the mineralization of new bone tissue. (boydbiomedical.com)
By mimicking the structure and composition of natural bone tissue and incorporating bioactive molecules, bioactive scaffolds provide a favorable environment for new bone growth and accelerate the healing process. (boydbiomedical.com)

Nature Biomedical Engineering. (chop.edu)
My Laboratory interfaces Biomedical Engineering and Orthopaedics. (rochester.edu)
In the Fall of 2004, I joined the faculty of the University of Rochester as an Assistant Professor in the Departments of Orthopaedics and Biomedical Engineering. (rochester.edu)
Nature Biomedical Engineering 2023 Jun 12. (boneandcancer.org)
Annals of biomedical engineering, 48(2), 595-605. (finmedtechnet.fi)

Lastly, in the case of creating synthetic cartilage to be used in joint spaces, high mechanical strength under compression needs to be an intrinsic property of the material. (wikipedia.org)
Additionally, the repaired tissue may not withstand the mechanical stresses of the joint, leading to a high risk of re-injury. (surgeonsync.com)
Lijie Grace Zhang is assistant professor in the Department of Mechanical and Aerospace Engineering and the Department of Medicine at the George Washington University, USA. (routledge.com)
Smart scaffolds for structural, mechanical, and functional support. (iqscloud.net)

These innovative materials offer a means to create the desired anisotropic architecture required for successful cartilage TE. (bvsalud.org)

Cytomechanical forces with rotational components strongly influence the osteogenic and chondrogenic differentiation. (biomedcentral.com)

Osteoarthritis (OA) is characterized by the progressive destruction of articular cartilage, which seriously restricts sports ability and impacts quality of life. (biomedcentral.com)
Loss of meniscal tissue is correlated with early osteoarthritis but few data exist regarding detailed biomechanical properties (e.g. viscoelastic behavior) of menisci in different species commonly used as animal models. (biomedcentral.com)
It is obvious that loss of meniscus tissue leads to focal overload of articular cartilage and finally to a higher incidence of osteoarthritis [ 1 , 3 ]. (biomedcentral.com)
Das Gupta S, Finnilä M, Karhula SS, Kauppinen S, Joukainen A, Kröger H, Korhonen RK, Thambyah A, Rieppo L, Saarakkala S. (2020) Raman microspectroscopic analysis of the tissue-specific composition of the human osteochondral junction in osteoarthritis: A pilot study. (finmedtechnet.fi)
Osteoarthritis and cartilage, 27(12), 1790-1799. (finmedtechnet.fi)
Automating three-dimensional osteoarthritis histopathological grading of human osteochondral tissue using machine learning on contrast-enhanced micro-computed tomography. (finmedtechnet.fi)

Initial studies indicate an immense potential for cell based strategies to enhance current orthopaedic approaches in skeletal tissue reconstruction. (openorthopaedicsjournal.com)
A number of traditional therapeutic approaches to repair skeletal tissue have been developed and hypothesised, but they have all presented with a variety of shortcomings. (openorthopaedicsjournal.com)
Although current treatment modalities offer high levels of success for many conditions, an ideal form of therapy might consist of regenerative approaches in which diseased or necrotic pulp tissues are removed and replaced with healthy pulp tissue to revitalize teeth. (bvsalud.org)

Growth factors are signaling molecules that regulate cell behavior and tissue development. (surgeonsync.com)

First, cartilage is an avascular structure in the body and therefore does not repair itself. (wikipedia.org)
Furthermore, man-made materials have a number of limitations that for the time-being limit their potential use for cartilage repair. (rochester.edu)
However, with the advent of osteochondral defect repair techniques, there is now a growing understanding of the importance of restoring the damaged cartilage and bone to achieve long-term relief and improved joint function. (surgeonsync.com)
Reparative techniques aim to promote the natural healing process of the body by creating an environment conducive to cartilage and bone repair. (surgeonsync.com)
ACI has shown promising results in terms of cartilage repair and pain reduction. (surgeonsync.com)
Therefore, it has the potential to serve as an injectable scaffold to repair bone fractures by promoting cell adhesion and function at the target location. (jove.com)
These studies integrate both basic scientists and clinicians to more effectively translate these basic science findings into new, innovative human health care protocols of using tissue engineering to repair skeletal tissues as in this accompanying figure. (case.edu)
Most of these paracrine secretions include soluble factors and exosomes, which regulate the repair and regeneration processes at sites of damage by affecting cell proliferation, migration, and differentiation [ 22 , 23 ]. (hindawi.com)
Optimization of IGF delivery systems will facilitate treatment application in cartilage repair and improve OA treatment efficacy. (biomedcentral.com)
Once a cartilage defect occurs, self-repair is not easy [ 3 ]. (biomedcentral.com)
Growth factors and their signaling pathways have recently attracted much attention in cartilage repair for OA treatment. (biomedcentral.com)
Insulin-like growth factor-1 (IGF-1), a member of a family of growth factors that are structurally closely related to pro-insulin, has shown profound effects on chondrocyte biological behavior and fundamentally regulates cartilage matrix metabolism during cartilage repair. (biomedcentral.com)
16. Buckley C.T., Hoyland J.A., Fujii K., Pandit A., Iatridis J.C. and Grad S. Critical aspects and challenges for intervertebral disc repair and regeneration - Harnessing advances in tissue engineering. (buckleylab.eu)
Learn, simplify and implement: developmental re-engineering strategies for cartilage repair. (unibas.ch)
In the future, will humans be able to bioprint living tissue to repair and replace ailing or diseased tissue? (advfn.com)
Chondroinduction is the main cartilage repair response to microfracture and microfracture with BST-CarGel: results as shown by ICRS-II histological scoring and a novel zonal collagen type scoring method of human clinical biopsy specimens. (howhelp.org)
Moreover, to produce acceptable structural and functional repair, all three kinds of tissues involved in osteochondral lesions, including subchondral bone, the osteochondral interface and articular cartilage, need to be reconstructed simultaneously ( 3 ). (spandidos-publications.com)
Bioactive agents, including cytokines and growth factors such as stromal cell-derived factor-1, platelet-derived growth factor, VEGF, and others, were shown to promote cell recruitment and have a helpful effect on the repair of articular cartilage injuries ( 8 , 10 , 11 ). (spandidos-publications.com)

Tissue engineering principles are used in order to create a non-degradable and biocompatible material that can replace cartilage. (wikipedia.org)
Its experimental origins date back to the 1970s with cell seeding of biocompatible materials to generate new tissues. (embs.org)
The first tissue-engineered, ear-shaped appendages made from bovine chondrocytes and biocompatible scaffolds by the Vacanti group were prone to deformation when xenografted onto immune-compromised mice, highlighting the lack of long-term stability [ 6 ]. (biomedcentral.com)

They were initially found in the bone marrow [ 11 ] but can also be found in other tissues, such as adipose, periosteum, muscle, placenta, and trabecular bone [ 12 ]. (hindawi.com)

Another example is articular cartilage damage as a result of trauma or chronic degenerative changes. (openorthopaedicsjournal.com)

She is also director of the Bioengineering Laboratory for Nanomedicine and Tissue Engineering at the university. (routledge.com)
A recent publication titled "Co-electrospun PCL/Zein membranes as scaffolds for articular cartilage engineering" by the ETH team was featured in MDPI Bioengineering. (biotrib.eu)

Connective Tissue Research, 59(4):381-392, 2018. (buckleylab.eu)
The papillary dermis is thinner, consisting of loose connective tissue that contains capillaries, elastic fibers, reticular fibers, and some collagen. (medscape.com)
The reticular dermis consists of a thicker layer of dense connective tissue containing larger blood vessels, closely interlaced elastic fibers, and coarse, branching collagen fibers arranged in layers parallel to the surface. (medscape.com)

Tissue Eng Part A. 2021 Jan 26. (case.edu)
Tissues & Wipes Market in India - India Tissues & Wipes Market Outlook, 2021", tissue paper market receives the maximum demand from institutional consumers such as hotels, restaurants, hospitals, and other commercial buildings. (powershow.com)

In a rat fracture model, IGF-1 combined with TGF-ß could stimulate chondrocyte proliferation and cartilage formation at the early stage of day 5 [ 8 ]. (biomedcentral.com)

While creating a useful synthetic cartilage material, certain challenges need to be overcome. (wikipedia.org)
Synthetic cartilage also needs to be stably attached to its underlying surface i.e. the bone. (wikipedia.org)
Synthetic cartilage can be composed of many different materials that mimic its functional properties. (wikipedia.org)

Biologically and mechanically driven design of an RGD-mimetic macroporous foam for adipose tissue engineering applications. (unibas.ch)

They provide a suitable environment for cell growth and can be loaded with growth factors to enhance tissue regeneration. (surgeonsync.com)
They play a crucial role in promoting cartilage and bone regeneration by stimulating cell proliferation, differentiation, and extracellular matrix synthesis. (surgeonsync.com)
After incubation, cell cultures were transfered to the different cutures systems: regular static tissue flasks (group I), spinner flasks (group II) and rotating wall vessels (group III). (biomedcentral.com)
The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
BC-Ppy scaffolds improved cell viability andattachment, promoting a desirable cardiomyoblast morphology. (ibecbarcelona.eu)
Scaffold Composition Determines the Angiogenic Outcome of Cell-Based Vascular Endothelial Growth Factor Expression by Modulating Its Microenvironmental Distribution. (unibas.ch)
Engineered mesenchymal cell-based patches as controlled VEGF delivery systems to induce extrinsic angiogenesis. (unibas.ch)
The combined efforts of cell biologists, material scientists, tissue engineers and reconstructive surgeons and associated converging technologies [ 1 ] in the 21st century have put us in an enviable position compared with our predecessors. (biomedcentral.com)
The extent of inflammatory cell accumulation correlated with tissue formation and polymer degradation but was also associated with leaflet thickening and decreased leaflet motion. (bvsalud.org)
Cellulose nanofibers (CNF) hydrogel has great potential as a cell-encapsulation delivery carrier for sustained release of paracrine factors and for tissue regeneration, with unique versatility for injection, scaffolding, and 3D bioprinting. (longdom.com)

Therefore, IGF-1 is the crucial factor in serum and synovial fluid that promotes cartilage matrix anabolism. (biomedcentral.com)
The presence of IGF-1 is crucial for maintaining cartilage integrity. (biomedcentral.com)

Alessio is investigating the Elucidation of Friction-Induced Failure Mechanisms in Fibrous Collagenous Tissues at ETH Zürich, Switzerland. (biotrib.eu)
Although MF-activated SSCs tended to form fibrous tissues, localized co-delivery of BMP2 and soluble VEGFR1 (sVEGFR1), a VEGF receptor antagonist, in a hydrogel skewed differentiation of MF-activated SSCs toward articular cartilage. (howhelp.org)

Langer and Vacanti 19 defined tissue engineering as an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function. (bvsalud.org)

My research focuses primarily on Musculoskeletal Tissue Engineering with an emphasis on challenging clinical problems and translational solutions. (rochester.edu)
Strategies for IGF delivery to chondrocytes and cartilage matrix are essential for its clinical application in OA treatment. (biomedcentral.com)
This is a multi-disciplinary approach that integrates molecular, biochemical and clinical techniques with developmental and engineering processes. (openorthopaedicsjournal.com)

Anatomy61

Organisms9

Diseases5

Chemicals and Drugs70

Analytical, Diagnostic and Therapeutic Techniques and Equipment50

Phenomena and Processes44

Disciplines and Occupations8

Technology, Industry, Agriculture30

Information Science3

Biomaterials24

Autologous7

Vitro14

Regenerative Medicine16

Hyaline7

Organs13

Hydrogels9

Vivo12

MSCs12

Defects11

Biomaterial scaffolds4

Stem cell13

Tendon2

Osteogenic differentiation3

Proliferation and differentiation2

Cardiac9

Biomimetic2

Extracellular matrix of cartilage2

Porous2

Articular cartilage repair2

Cells31

Bone tissue3

Biomedical Engineering5

Mechanical4

Anisotropic1

Chondrogenic1

Osteoarthritis6

Approaches3

Molecules1

Repair18

Biocompatible3

Marrow1

Degenerative1

Bioengineering2

Connective3

20212

Chondrocyte1

Synthetic3

Adipose tissue1

Cell10

Crucial2

Fibrous2

Vacanti1

Clinical3