• Less quantity of transplanted mesenchymal stem cells (MSCs) influences the therapeutic effects on radiation-induced lung injury (RILI). (hindawi.com)
  • Previous studies have demonstrated that MSCs overexpressing Chemokine (C-X-C motif) receptor 4 (CXCR4) could increase the quantity of transplanted cells to local tissues. (hindawi.com)
  • It has been observed that mesenchymal stem cells (MSCs) could home and engraft within the injured tissues and differentiate into vascular cells and alveolar cells to regenerate the damage in the irradiated mouse lung [ 10 ]. (hindawi.com)
  • Mesenchymal stem cells (MSCs) also known as mesenchymal stromal cells or medicinal signaling cells are multipotent stromal cells that can differentiate into a variety of cell types, including osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells) and adipocytes (fat cells which give rise to marrow adipose tissue). (wikipedia.org)
  • While the terms mesenchymal stem cell (MSC) and marrow stromal cell have been used interchangeably for many years, neither term is sufficiently descriptive: Mesenchyme is embryonic connective tissue that is derived from the mesoderm and that differentiates into hematopoietic and connective tissue, whereas MSCs do not differentiate into hematopoietic cells. (wikipedia.org)
  • Mesenchymal stem cells (MSCs), a term first coined by Arnold I. Caplan in 1991, are characterized morphologically by a small cell body with a few cell processes that are long and thin. (wikipedia.org)
  • However, MSCs are found in much higher concentration in the Wharton's jelly compared to cord blood, which is a rich source of hematopoietic stem cells. (wikipedia.org)
  • These cells have several advantages over bone-marrow-derived MSCs. (wikipedia.org)
  • MSCs have been seen to even differentiate into neuron-like cells, but doubt remains about whether the MSC-derived neurons are functional. (wikipedia.org)
  • Conclusions: The cells isolated from dental pulp exhibited characteristics compatible with those expected for mesenchymal stem cells (MSCs) and are good candidates for cell therapy applications and tissue bioengineering. (bvsalud.org)
  • The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
  • The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
  • MSCs have a lower ability to differentiate into osteoblasts but a higher ability to differentiate into adipocytes, which could be linked to myeloid malignancies ( Woods and Guezguez, 2021 ). (techscience.com)
  • Open up in another window Shape 1 Phase comparison microscopic images displaying major cultures of human being bone tissue marrow produced mesenchymal stem cells (hBMMSCs) at passages P0 Mcl1-IN-9 (A) and P1 (B). Non-adherent cells are indicated by dark arrows in P0 (A). The hBM-MSCs at P1 exhibited short and epitheloid spindle shaped and morphology. (mingsheng88.org)
  • Mesenchymal stem cells (MSCs) are the most commonly used cells in tissue engineering and regenerative medicine. (frontiersin.org)
  • MSCs can promote host tissue repair through several different mechanisms including donor cell engraftment, release of cell signaling factors, and the transfer of healthy organelles to the host. (frontiersin.org)
  • Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)
  • Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
  • MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
  • Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
  • The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) are the adult stem cells that have been studied in recent years and used to treat many diseases. (biomedcentral.com)
  • Thus, these properties of MSCs make these cells potentially ideal candidates for tissue technology. (stemcellsgroup.com)
  • Arnold Caplan (1991), coined the term "mesenchymal stem cell and stated that the cells as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies. (stemcellsgroup.com)
  • However, the first clinical trials of MSCs were completed in 1995 when a group of 15 patients were injected with cultured MSCs to test the safety of the treatment. (stemcellsgroup.com)
  • Mesenchymal stem/stromal cells (MSCs) can be isolated from neonatal tissues, most of which are discarded after birth, including placental tissues, fetal membranes, umbilical cord, and amniotic fluid. (stemcellsgroup.com)
  • Thus, neonatal tissues are easily available and they have biological advantages in comparison to adult sources that make them a useful source for stem cells including MSCs. (stemcellsgroup.com)
  • In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
  • Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)
  • iii) absence of several haematopoietic and endothelial markers (ie, CD45, CD34, CD11b or CD14, CD79 or CD19 and HLA-DR in human MSCs). (bmj.com)
  • 1 Unique MSC-specific markers have not yet been identified, and MSCs constitute a heterogeneous cell population, including both multipotent (stem) cells and progenitor cells and might even contain pluripotent cell fractions. (bmj.com)
  • 2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)
  • On infusion, culture-expanded MSCs regulate inflammatory and immune responses and tissue repair. (bmj.com)
  • Following early observations that MSCs inhibit T-cell proliferation, 9 MSCs were found to interact with the majority of innate and adaptive immune cells. (bmj.com)
  • Suture mesenchymal stem cells (SuSCs), a heterogeneous stem cell population, belong to mesenchymal stem cells (MSCs) or skeletal stem cells (SSCs), with the ability to self-renew and undergo multi-lineage differentiation. (encyclopedia.pub)
  • The MSCs and osteoprogenitors residing along the OFs keep proliferating, subsequently differentiate into osteoblasts, and contribute to the new bone formation through intramembranous ossification [ 4 ] [ 5 ] , which happens with a direct differentiation into osteoblasts from MSCs and/or osteogenic precursors without assuming a chondrogenic fate. (encyclopedia.pub)
  • Mesenchymal stem cells (MSCs) are under investigation to treat autoimmune and degenerative disorders due to their immunomodulatory and regenerative properties. (biomedcentral.com)
  • Autologous bone marrow MSCs (BMMSCs), isolated, cultured, and expanded, were administered by intrathecal (IT) injection in the cisterna magna intravenously (IV) and by intra-arterial (IA) injection in the right carotid artery. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) are multipotent cells with the potential to differentiate into bone, cartilage, fat and muscle cells and are being investigated for their utility in cell-based transplantation therapy. (biomedcentral.com)
  • In addition, functional integration of QD labeled MSCs in an in vitro cardiomyocyte co-culture was assessed. (biomedcentral.com)
  • Finally, in a cardiomyocyte co-culture QD labeled MSCs were easy to locate and formed functional cell-to-cell couplings, assessed by dye diffusion. (biomedcentral.com)
  • Transplanted MSCs are expected to engraft, differentiate and remodel in response to the surrounding cardiac microenvironment resulting in tissue regeneration and functional repair. (biomedcentral.com)
  • In part, this is due to limited methods to track MSCs in vivo , precluding long-term functional studies of transplanted cells. (biomedcentral.com)
  • We assessed the ability to track QD labeled MSCs in an in vitro cardiomyocyte co-culture. (biomedcentral.com)
  • Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (stemcellslab.net)
  • Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (stemcellslab.net)
  • These studies used various ways to label culture-expanded MSCs, and to track them in different tissues over time. (stemcellslab.net)
  • The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (stemcellslab.net)
  • and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (stemcellslab.net)
  • These stem cells are capable of differentiating into chondrocytes, cardiomyocytes, melanocytes, and hepatocyte‐like cells in vitro. (wikipedia.org)
  • Objective: To characterize pulp stem cells and evaluate their capacity for expansion and differentiation in vitro. (bvsalud.org)
  • Pulp stem cells (PSCs) are able to form ectopic dentin in vitro and in vivo and also to generate a dentine-pulp complex composed of a mineralized matrix, with dentinal tubules that are aligned and filled with odontoblastic prolongations, containing vascularized pulp tissue, in a similar arrangement to that observed in natural dental structures 3,6 . (bvsalud.org)
  • The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
  • Identifying causal variants and genes from human genetic studies of hematopoietic traits is important to enumerate basic regulatory mechanisms underlying these traits, and could ultimately augment translational efforts to generate platelets and/or red blood cells in vitro. (biomedcentral.com)
  • Cost-effective blood cell generation will require novel strategies based on better knowledge of underlying mechanisms driving in vitro development. (biomedcentral.com)
  • In vitro hematopoietic systems might be improved by identifying novel factors from human genetic studies. (biomedcentral.com)
  • Granulosa cells were isolated and treated with SiAmh interference and hPMSC supernatant to observe the effects of AMH expression on granulosa cell apoptosis in vitro. (biomedcentral.com)
  • In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
  • a) The cells should exhibits plastic adherence (b) The cell should possess specific set of cell surface markers, i.e. cluster of differentiation (CD) 73, D90, CD105 and lack expression of CD14, CD34, CD45 and human leucocyte antigen-DR (HLA-DR). (c) The cells should have the ability to differentiate in vitro into adipocyte, chondrocyte and osteoblast. (stemcellsgroup.com)
  • In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
  • An in vitro study on the effect of hyperglycemia on the proliferation and viability of the cultured EPCs was also performed. (biomedcentral.com)
  • When glucose was supplemented in the culture in vitro , there was a negative effect on the proliferation and viability of EPCs, in a dose-dependent manner, whereas the enhancement of apoptosis was observed. (biomedcentral.com)
  • In vitro hyperglycemic effect using various glucose concentrations on the viability, proliferation and apoptosis of cultured EPCs from both diabetic patients and healthy controls were also performed. (biomedcentral.com)
  • Methods This study used human peripheral blood mononuclear cells from healthy donors and in vitro co-culture of MM cells and BM stromal cells to recapitulate the resistant BM niche. (bmj.com)
  • Quantum Dots (QDs) offer an alternative to organic dyes and fluorescent proteins to label and track cells in vitro and in vivo . (biomedcentral.com)
  • Here, we investigate the cytotoxic effects of in vitro QD labeling on MSC proliferation and differentiation and use as a cell label in a cardiomyocyte co-culture. (biomedcentral.com)
  • Fluorescent QDs label MSC effectively in an in vitro co-culture model. (biomedcentral.com)
  • Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)
  • CXCR4 is a G protein-linked seven transmembrane spanning receptor that has been identified as a receptor of stromal cell-derived factor-1 (SDF-1) for stem cells [ 22 - 24 ]. (hindawi.com)
  • Stromal cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside. (wikipedia.org)
  • They are sometimes referred to as bone marrow stromal stem cells. (wikipedia.org)
  • The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
  • The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)
  • Mesenchymal stromal cells: a novel therapy for the treatment of chronic obstructive pulmonary disease? (bmj.com)
  • MM cells co-cultured with BM stromal cells displayed resistance to reovirus-induced oncolysis and bystander cytokine-killing but remained susceptible to killing by reovirus-activated NK cells and MM-specific cytotoxic T lymphocytes. (bmj.com)
  • Another statement showed the preadipocyte- and endothelial cell-derived stromal-derived element-1 (CXCL12), mediated early infiltration of CD4+ T lymphocytes in obesity, which preceded the increase of macrophages in adipose cells of mice on HFD (101). (exposed-skin-care.net)
  • Hu G, Liu P, Feng J, Jin Y. Transplantation with Bone Marrow Stromal Cells Promotes Wound Healing Under Chemotherapy through Altering Phenotypes. (ijbs.com)
  • Herein we characterized human fetal bone marrow stromal cells (hBMSCs) during wound healing in mice treated with cyclophosphamide (CTX). (ijbs.com)
  • 1, 2 Recently, Prolotherapists have begun to utilize the potential of autologous adipose (fat)-derived stem/stromal cells (AD-SC) within non-manipulated fat graft scaffolding, combined with high-density PRP concentrates (HD-PRP) to provide a potent biological therapeutic combination. (journalofprolotherapy.com)
  • Recognition of the vast number of undifferentiated cells associated with the stromal vascular fraction has resulted in extensive research demonstrating the heterogeneity of such cells, and their ability to participate in production of all mesodermal-derived tissues. (journalofprolotherapy.com)
  • 7 There has been some variation and question regarding the correct terminology for this population of stromal adipose cells. (journalofprolotherapy.com)
  • At first, the mesenchymal stem cell was thought to be the primary component of this undifferentiated cell type, however it is now evident that within the adipose extracellular matrix are also adipocytic precursors (known as progenitor cells) adherent to adipocytes, and in close approximation to a variety of additional undifferentiated multipotent and pluripotent cells, including pericytes and endothelial cells, all thought to play important roles in mesenchymal-stromal derived tissue regeneration. (journalofprolotherapy.com)
  • Therefore we have chosen to use the term "adipose-derived stem/stromal cells" (AD-SC's), rather than simply "mesenchymal stem cells. (journalofprolotherapy.com)
  • This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (stemcellslab.net)
  • Autologous CD34 + cells were transplanted in 15 patients with tibia nonunion and 10 patients with femur nonunion, who were followed up for 52 weeks post transplantation. (bvsalud.org)
  • CONCLUSIONS: Our findings strongly suggest that autologous CD34 + cell transplantation is a novel treatment option for fracture nonunion. (bvsalud.org)
  • Transplantation of hBMMSCs as pellets rather than as cell suspensions to the cartilage defect site would therefore support their viability and may aid enhanced cartilage regeneration. (mingsheng88.org)
  • Both cells have common applications in various cell-based therapies such as tissue regeneration and transplantation. (differencebetween.com)
  • This study was performed to determine the effects of human placenta mesenchymal stem cell (hPMSC) transplantation on granulosa cell apoptosis and anti-Müllerian hormone (AMH) and follicle-stimulating hormone receptor (FSHR) expression in autoimmune drug-induced premature ovarian failure (POF) mice. (biomedcentral.com)
  • granulosa cell apoptosis was decreased following hPMSC transplantation. (biomedcentral.com)
  • hPMSC transplantation can significantly improve the serum levels of high gonadotropin and low estrogen of POF mice, promote follicular development, inhibit excessive follicular atresia and granulosa cell apoptosis, and improve the ovarian reserve capacity. (biomedcentral.com)
  • Source material for hematopoietic (bone marrow) transplantation is in great demand as at least 20,000 allogeneic transplants are performed each year. (utoronto.ca)
  • Stem cell transplantation is a promising strategy for delayed wound healing caused by chemotherapy. (ijbs.com)
  • Nevertheless, myelosuppression can be reversed by autologous stem cell transplantation (SCT), even without additional toxicity ( 4 , 5 ). (ijbs.com)
  • In order to eliminate the interference from the transplanted cells themselves, fetal stem cells were used for transplantation because the proliferation and differentiation potentials of fetal stem cells are superior to the adult ones ( 8 ). (ijbs.com)
  • In these studies, however, transplanted cells were followed, if at all, at the site of transplantation, and biodistribution was not an issue. (stemcellslab.net)
  • The standard test to confirm multipotency is differentiation of the cells into osteoblasts, adipocytes and chondrocytes as well as myocytes. (wikipedia.org)
  • Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
  • Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
  • Results: It was observed that the pulp stem cells exhibited the capacity to adhere to plastic and a high rate of expansion and, after detection with specific stains, it was shown that the cells were capable of differentiation into osteoblasts and chondroblasts, but not into adipocytes. (bvsalud.org)
  • Stem cells are cells with a low degree of differentiation that have the capacity to reproduce and can generate differentiated cells of several different types of tissues 1 . (bvsalud.org)
  • The objective of this study is to isolate and characterize stem cells from the pulp of permanent third molars and analyze the cells obtained in terms of their morphology and chondrogenic, osteogenic and adipogenic cell differentiation. (bvsalud.org)
  • It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
  • The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)
  • The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
  • Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
  • We show here that these novel intrathymic DCs are truly myeloid, as they arise from CD34 + early thymic progenitors through CD34 lo intermediates which have lost the capacity to generate T cells, but display myelomonocytic differentiation potential. (ashpublications.org)
  • These cells evolve from the differentiation of neural progenitor cells (NPCs). (differencebetween.com)
  • They play a role in cell development and differentiation. (differencebetween.com)
  • Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
  • In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
  • Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)
  • pointed out that Mage-D1 may participate in the proliferation of bone marrow mesenchymal stem cells (BMSCs) and the differentiation of odontoblasts through the NF-κB signalling pathway 21 . (researchsquare.com)
  • Depending on the tissue of origin, they may have a higher preference for differentiation into one particular cell type and a lower preference for another one, but they all still retain the differentiation potential for the mesenchymal lineage. (promocell.com)
  • Which plates does PromoCell recommend as 96-well U bottom suspension plates for chondrogenic differentiation of Human Mesenchymal Stem Cells? (promocell.com)
  • For chrondrogenic differentiation it is important that the cells do not adhere to the wells. (promocell.com)
  • During differentiation, the cells form spheroids which float in the medium. (promocell.com)
  • mice could be achieved by reconstituting CD8+ T cells in deficient hosts suggesting that practical plasticity was still possible after thymic development differentiation and emigration. (siamtech.net)
  • Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
  • We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
  • Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
  • Promotes osteogenic, adipogenic, and chondrogenic differentiation of human mesenchymal cells (Jaiswal et al. (stemcell.com)
  • Promotes osteogenic, adipogenic, and chondrogenic differentiation of mouse mesenchymal cells (Tropel et al. (stemcell.com)
  • Promotes differentiation of mature hepatocytes from mouse and human embryonic stem (ES) cells (Cai et al. (stemcell.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells. (thermofisher.com)
  • CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. (thermofisher.com)
  • Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. (thermofisher.com)
  • They include endothelial cells, fibroblasts, and keratinocytes. (frontiersin.org)
  • Endothelial progenitor cells (EPCs) play an important role in vascular repair and a decrease in the number of EPCs is observed in type 2 diabetes. (biomedcentral.com)
  • Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. (lww.com)
  • 1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
  • GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
  • Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
  • studies indicated that RANTES is Purpureaside C an adipokine that can be produced by adipocytes and takes on an important part in T cell migration, suggesting a potential part of the RANTES/CCR5 axis in adipose T cell build up in obesity (24). (exposed-skin-care.net)
  • Indeed, the principal adipocytes isolated from obese mice could induce antigen-specific Compact disc4+ T cell activation (58). (exposed-skin-care.net)
  • further defined that mostly huge adipocytes from obese adipose tissues exhibited an increased expression degree of MHCII substances and acted as APCs to activate Compact disc4+ T cells to secrete IFN- (103). (exposed-skin-care.net)
  • Taken together, one important mechanism for obese adipose CD4+ T cell activation may be mediated through MHCII expressed on ATMs and adipocytes. (exposed-skin-care.net)
  • The term encompasses multipotent cells derived from other non-marrow tissues, such as placenta, umbilical cord blood, adipose tissue, adult muscle, corneal stroma, or the dental pulp of deciduous (baby) teeth. (wikipedia.org)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • Progenitor cells are an intermediary step in the development of mature cells in tissues, organs, blood, and the central nervous system. (differencebetween.com)
  • Therefore, progenitor cells are necessary for repairs and to maintain tissues after an injury. (differencebetween.com)
  • They can create new tissues and even whole organs from a few stem cells. (differencebetween.com)
  • The first source of Mesenchymal stem cells was in the bone marrow and considered to be the gold standard for clinical research, although various other sources have being discovered which include: Adipose tissue, Dental pulp, Mobilised Peripheral blood, Amniotic fluid, Joint synovium, synovial fluid, Endosteum, Periosteum, Menstrual blood and birth-derived tissues. (stemcellsgroup.com)
  • Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
  • How do mesenchymal stem cells from different tissues differ in terms of their biological function? (promocell.com)
  • In obese humans, adipocyte-secreted CCL20 may contribute to the deposition of Compact disc4+ helper and Compact disc8+ cytotoxic T lymphocytes within adipose tissues, possibly via connections with CCR6 which was upregulated on T cells in obese adipose tissues (100). (exposed-skin-care.net)
  • However, the main element substances that mediate T cell infiltration into adipose tissues in maturing remain to become discovered. (exposed-skin-care.net)
  • Activation of Typical T Cells in Adipose Tissues Compact disc4+ Purpureaside C T Cell Activation TCRs recognize the current presence of a particular antigen by binding to brief peptide sequences in the antigen that's shown on APCs. (exposed-skin-care.net)
  • Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)
  • In recent years, with increasing interest being directed toward cultured cell-based production of vaccines, and in vivo proteins such as enzymes, hormones, antibodies and cytokines, as well as cell grafts for use in regenerative medicine, greater focus has been placed on efficient and convenient methodologies for mass cell culturing. (justia.com)
  • Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
  • Methods: Pulp tissue was collected from permanent third molars and digested and then the cells were seeded onto plates containing HDMEM medium. (bvsalud.org)
  • An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
  • The original amount of cells in major monolayer cultures different from 1.4 0.4 106 to at least one 1.9 Mcl1-IN-9 0.6 106 cells (from 5 mL bone tissue marrow aspirate Mcl1-IN-9 cultured in three T175 cm2 flasks). (mingsheng88.org)
  • Open up in another window Shape 2 Representative Fluorescent triggered cell-sorting (FACS) evaluation showing the Compact disc marker expression design in human being bone tissue marrow mesenchymal stem cells (hBMMSCs). (mingsheng88.org)
  • However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
  • Progenitor cells are capable of differentiating into cells that belong to the same tissue or organ of each progenitor cell. (differencebetween.com)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • In our previous studies, EMSCs were obtained from rat embryonic facial process tissue by fluorescence p75 neurotrophin receptor (p75NTR) activated cell sorting, providing a good stem cell model for studies of dental mineralization. (researchsquare.com)
  • Fibroblasts are activated connective tissue cells characterized by synthesis of proteins of the fibrous matrix, particularly the collagens . (ipfs.io)
  • When tissue is injured, the predominant mesenchymal cells, the fibroblast, have been believed to be derived from the fibrocyte or possibly from smooth muscle cells lining vessels and glands. (ipfs.io)
  • Recently, the term "fibrocyte" has also been applied to a bloodborne cell able to leave the blood, enter tissue and become a fibroblast. (ipfs.io)
  • Worldwide, 50,000 hematopoietic stem cell transplant (HSCT) procedures are undertaken annually to replace a person's blood-forming cells - hematopoietic stem cells (HSCs) - to treat blood disorders and cancers, yet two-thirds of patients who need HSCT lack matched donor tissue. (utoronto.ca)
  • Pathogenic transformation of Tregs in addition has been defined in various other experimental versions (25 26 Furthermore both maintenance of suppressive actions in peripheral tissue and the legislation of endogenous creation of IL-6 by nTregs had been been shown to be dependent on the current presence of Compact disc8+ T cells (21). (siamtech.net)
  • One great advantage of MSC is that these cells may be directly obtained from individual patients, thereby eliminating the complications associated with immune rejection of allogenic tissue and infectious diseases. (biomedcentral.com)
  • Proteomics is a powerful approach to study the whole set of proteins expressed in an organism, organ, tissue or cell resulting in valuable information on physiological or pathological state of a biological system. (oaji.net)
  • Tissue from the upper or lower respiratory tract, sputum samples, and samples obtained by nasopharyngeal washing, bronchoalveolar lavage, and biopsy may be submitted for viral culture. (medscape.com)
  • indicated that ATMs colocalized with T cells in lymphoid clusters within adipose tissue and may act as APCs, which express high levels of MHCII and also costimulatory molecules and process and present antigens to induce CD4+ T-cell proliferation and activation in adipose tissue of obese mice (29, 68, 105). (exposed-skin-care.net)
  • However, its role in aging-related adipose tissue CD4+ T cell activation remains to be investigated. (exposed-skin-care.net)
  • CD8+ T Cell Activation Compared to CD4+ T cells, CD8+ T cells show a greater increase in adipose tissue in obesity and in aging (31, 43, 106). (exposed-skin-care.net)
  • Similar to CD4+ T cells, CD8+ T cells exhibit effector memory or effector phenotypes expressing elevated levels of IFN- in obese adipose tissue (31, 44). (exposed-skin-care.net)
  • The mechanism for CD8+ T cell activation in adipose tissue is not fully understood. (exposed-skin-care.net)
  • showed that adipose tissue from obese mice induced proliferation of splenic CD8+ T cells, indicating a CD8+ T cell-activating environment in obese adipose tissue (31). (exposed-skin-care.net)
  • Indeed, CD8+ T cells from mouse adipose tissue respond to cytokines and become activated and proliferate under stimulation of IL-12 and IL-18, which are mainly produced by APCs and are elevated in obese adipose tissue (44). (exposed-skin-care.net)
  • One explanation for deficient repair is when undifferentiated adult stem repair cells are inadequate in number or cannot be stimulated within the damaged tissue site. (journalofprolotherapy.com)
  • With improved understanding of tissue healing and regeneration, stem cell Prolotherapy is gaining significant clinical importance and potential. (journalofprolotherapy.com)
  • Hematopoietic stem cells (HSCs) are immature cells found in the peripheral blood and bone marrow. (differencebetween.com)
  • Peripheral blood fibrocytes from burn patients: identification and quantification of fibrocytes in adherent cells cultured from peripheral blood mononuclear cells. (ipfs.io)
  • RNA was prepared separately from peripheral blood mononuclear cells (PBMC) and granulocytes to UNC1215 identify differentially expressed genes using whole genome microarrays. (tech-strategy.org)
  • Here, a combination of different cell surface markers is used to separate the rare HSCs. (differencebetween.com)
  • Pre-GEPCOT cells could not form neurospheres but expressed the stem cell markers Slc1a3-CreER T , GFAP-CreER T2 , Sox2 CreERT2 , and Gli1 CreERT2 and were long-lived in vivo. (elifesciences.org)
  • term pre-GEPCOT cells (based on an acronym of the markers used to isolate the cells), were long-lived and quiescent, but they lacked the ability to form colonies in culture. (elifesciences.org)
  • These cells have been reported to express the hematopoietic cell surface markers CD34+, CD45+, as well as collagen . (ipfs.io)
  • The group plans to identify better markers to track and purify key cell intermediates as well as uncover key genes/pathways that lead to HSC development. (utoronto.ca)
  • Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
  • To validate this putative candidate gene and to explore its function, we used CRISPR/Cas9 genome editing to discover that cultured TPM1 -deficient induced pluripotent stem cells enhanced hematopoietic progenitor cell formation. (biomedcentral.com)
  • In this study, the finding that a significant proportion of all dendritic cells (DCs) resident in vivo in the human postnatal thymus displayed a myeloid-related phenotype prompted us to re-examine the developmental origin of thymic DCs, a cell type hitherto considered to represent a homogeneous lymphoid-derived population. (ashpublications.org)
  • We also demonstrate that phenotypically and functionally equivalent myeloid precursors devoid of T-cell potential do exist in vivo in the postnatal thymus. (ashpublications.org)
  • 8 As these latter DCs derive from an intrathymic lymphoid-restricted progenitor able to generate also T, B, and NK cells, but not myeloid cells, upon transfer in vivo, 5 , 9 , 10 they have hitherto been considered of lymphoid origin. (ashpublications.org)
  • 2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
  • In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
  • Through them, the cells modify their microenvironment and the behavior of neighboring cells. (frontiersin.org)
  • While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
  • As part of the more general topic of stem cell biology, a number of studies have suggested that the blood contains marrow derived cells that can differentiate into fibroblasts. (ipfs.io)
  • During the recovery phase, the flow of cells from the marrow decreases, with a resultant decrease in the number of neutrophils. (medscape.com)
  • This is followed rapidly by egress of cells from the marrow, resulting in an increase in the TBGP and blood neutrophilia. (medscape.com)
  • Multiple myeloma (MM) is a tumor of terminally differentiated plasma cells which expand in the bone marrow (BM). (bmj.com)
  • Treatment for this condition remains difficult and largely ineffective, because the growth of cells in the wound is inhibited ( 2 ) and chemotherapy usually leads to myelosuppression, the latter of which further compromises the migration of multipotent cells from the bone marrow ( 3 ). (ijbs.com)
  • In the 1970s, Friedenstein and colleagues [1] first reported that locally applied culture-expanded populations of bone marrow stroma-derived fibroblastic cells remained at their injection sites under the kidney capsule, where an ectopic hematopoiesis was initiated. (stemcellslab.net)
  • Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
  • Similarly, they are characterized by an extensive capacity for self-renewal, proliferation, potential to differentiate into multiple lineages and their immune-modulatory role on various cells. (stemcellsgroup.com)
  • Nevertheless, with following passages where standard monolayer cultures had been acquired, the cell amounts could be extended to 2.1 0.4 106 cells per T175 cm2 flask. (mingsheng88.org)
  • Cells generally exist as three-dimensional aggregates in the body, but in classical plate culturing, cells are cultured in a monolayer fashion with the cells attached to a vessel. (justia.com)
  • In the central nervous system of humans, there are three types of fully differentiated cells known as neurons, astrocytes, and oligodendrocytes. (differencebetween.com)
  • Neurons that arise in the adult nervous system originate from neural stem cells and neural progenitor cells. (elifesciences.org)
  • Neural stem cells can also give rise to neural progenitor cells, which proliferate rapidly during their short lives and then 'differentiate' into neurons or glia. (elifesciences.org)
  • Since neurosphere-forming cells can self-renew and differentiate into neurons and glia, the ability of cells to form neurospheres has generally been taken as evidence that they are stem cells. (elifesciences.org)
  • Particularly, NME is an idiopathic inflammatory disease of the CNS that is characterized by prominent necrosis and infiltration of inflammatory cells, including lymphocytes, plasma cells, and monocytes or histiocytes into the cerebral cortex and/or white matter, hippocampus, thalamus, and leptomeninges. (biomedcentral.com)
  • This was associated with increased infiltration of macrophages, lymphocytes, and mast cells. (oaji.net)
  • In addition to a role in adaptive immunity, memory CD8+ T cells are involved in innate immunity, being able to become activated and to proliferate under cytokine stimulation (107, 108). (exposed-skin-care.net)
  • MSC co-culture with skin fibroblasts from mitochondrial disease patients rescued aberrant mitochondrial morphology from a fission state to a more fused appearance indicating an effect of MSC co-culture on host cell mitochondrial network formation. (frontiersin.org)
  • Commonly, fibroblasts express smooth muscle actin , a form of actin first found in smooth muscle cells and not found in resting fibrocytes. (ipfs.io)
  • As many as 1 in 100 cells collected during amniocentesis are pluripotent mesenchymal stem cells. (wikipedia.org)
  • However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)
  • At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)
  • CRISPR/Cas9-mediated TPM1 knockout in human induced pluripotent stem cells (iPSCs) enhanced hematopoietic progenitor development, increasing total megakaryocyte and erythroid cell yields. (biomedcentral.com)
  • Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
  • 1-3 These nonoverlapping functions have been proposed to result from the actions of 2 major DC populations which have been characterized as myeloid and lymphoid DCs, respectively, on the basis of their anatomical localization and cell-surface phenotypes and, ultimately, of their distinct developmental origin. (ashpublications.org)
  • HSCs develop into different types of blood cells in lines called myeloid and lymphoid , which are involved in dendritic cells formation. (differencebetween.com)
  • Lymphoid cells include T cells, B cells, innate lymphoid cells, and natural killer cells. (differencebetween.com)
  • These multipotent progenitor cells differentiate into either common myeloid progenitor (CMP) or common lymphoid progenitor cells (CLP). (differencebetween.com)
  • we also demonstrated the safety and efficacy of this cell therapy in an earlier phase I/II clinical trial conducted on seven patients with fracture nonunion. (bvsalud.org)
  • This promising field may open new avenues to modulate inflammatory responses and to preserve blood cell homeostasis in the clinical setting. (haematologica.org)
  • These cell types are used in cell culture studies to analyze cellular responses in different clinical and physiological settings. (differencebetween.com)
  • In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
  • The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
  • ar4280-S8.PDF (1.7M) GUID:?3B4F6A60-6238-4C1B-845B-025DC69E0C96 Abstract Introduction The attainment of remission has become an important end point for clinical trials in juvenile idiopathic arthritis (JIA), although we do not yet have a full understanding of what remission is at the cell and molecular level. (tech-strategy.org)
  • In the present study, we conducted overexpressing CXCR4 human umbilical cord mesenchymal stem cell (HUMSC) therapy for RILI. (hindawi.com)
  • Despite advances in using umbilical cord blood, donor material remains restricted by limited stem cells and the lack of ethnic diversity to provide sufficiently matched material. (utoronto.ca)
  • Embryonic stem cells isolation primarily requires the consent of donors and can include the killing of fertilized eggs. (techscience.com)
  • Analysis of the cellular phenotype showed that the cells were negative for CD45, CD69, CD117 and HLADR, and positive for CD13, CD44, CD73, CD90 and CD105. (bvsalud.org)
  • We prospectively identified, and isolated by flow cytometry, adult mouse lateral ventricle subventricular zone (SVZ) NICs as Glast mid EGFR high PlexinB2 high CD24 −/low O4/PSA-NCAM −/low Ter119/CD45 − (GEPCOT) cells. (elifesciences.org)
  • Moreover, although interleukin 7 (IL-7) supports the generation of such myeloid intermediates, we show that their developmental branching from the main intrathymic T-cell pathway is linked to the up-regulation of the myelomonocytic granulocyte macrophage-colony-stimulating factor (GM-CSF) receptor, to the down-regulation of the IL-7 receptor and to the lack of pre-T-cell receptor α (pTα) gene transcriptional activation. (ashpublications.org)
  • In the lack of or disturbance with MHC I-CD8 connections the regulatory actions of nTregs had been altered not merely leading to the increased loss of suppression however in their transformation to pathogenic IL-13-making Compact disc4+ T effector cells improving lung allergic replies in receiver mice (5). (siamtech.net)
  • Previously the essential part of GITR in the conversion of naturally happening CD4+CD25+ T regulatory cells to pathogenic CD4+ T effector cells was implicated from the abrogation of enhancement of lung sensitive response following administration of anti-GITRL antibody (5). (siamtech.net)
  • Especially in drug discovery, most such assays have been geared toward a particular reaction-whether it is recognition of a cell surface marker, triggering endocytosis, or exciting or inhibiting a phosphorylation cascade. (genengnews.com)
  • He said there was a huge interest in label-free assays among the participants of the recent SMi Conference on "Cell Based Assays" in London, where he discussed SymCel's pending entry into the field. (genengnews.com)
  • Because the assays require no labels they are noninvasive and nondestructive to the cells, inexpensive, simple, and quick to set up and use. (genengnews.com)
  • SymCel calScreener sample vials in close up: calScreener offers calorimetry-based cell assays in a microtiter plate format. (genengnews.com)
  • With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
  • Conclusions: hBMMSC cell pellets are better protected from temperature alterations compared to cell suspensions. (mingsheng88.org)
  • The methods proposed are cutting-edge and no other team in the world has this combination of technologies, approaches and expertise to make advances in hematopoietic cell therapies. (utoronto.ca)
  • However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. (lww.com)
  • However, for cell therapies MSC have to be expanded and/or manipulated to obtain a sufficient amount of cells that can be subsequently used for treatment. (biomedcentral.com)
  • Dendritic cells (DCs) are hematopoietic-derived highly specialized antigen-presenting cells (APCs) that display potent ability to induce both specific immune responses and deletion of potentially autoreactive T cells. (ashpublications.org)
  • This team, which brings together expertise in stem cell biology, cell biology, developmental signalling pathways, and genetic screens, aims at improving methods to achieve generation of HSC. (utoronto.ca)
  • As a solution for the issue of oxygen supply, it has been attempted to increase the oxygen concentration by utilizing microbubbles (PTL 1) or to employ methods for uniformly supplying oxygen in microcarrier culturing (PTL 2). (justia.com)
  • Methods of culturing while exposing the cell culture support to a gas phase have also been attempted. (justia.com)
  • To identify putative causal genes from these data, we performed computational modeling using available genome-wide association datasets for platelet and red blood cell traits. (biomedcentral.com)
  • Under induction by mineralized culture medium, Mage-D1 promotes osteogenesis and tooth-forming ability. (researchsquare.com)